pycom16g05350

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0002060
Physical Location & Seq
Forward (+)
3470460 .. 3472264
1805 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g05350.6

Sequence Viewer

Length: 1170 bp
ATGTGTGGTGCACCAGCAAAGACAGTGGGGTGGCGAGATCCTGGATTTATACACACTGCTTTTCTGAAGGAATTGTGGCCCAACACAGTGTACACCTACAAGGTTGGACACAGATTGCCAAATGGTTCCCATATTTTGAGTCAAGAATACAAGTTCAGAGCATCTCCATATCCTGGTCAAAATTCTGTTCAACGCGTTGTCATCTTCGGTGACATGGGAAAGGATGAAGCTGATGGATCCAATGAATATAACCAATACCAGCGTGGCTCTCTCAACACAACTAAGCAGCTAATCCAAGACTTAGAGAAAATTGATATCGTTTTCCACATTGGGGATATATGTTACGCAAATGGATACCTTTCGCAGTGGGACCAATTCACGGCACAGGTTGAGCCGATAGCATCTGCTGTTCCTTACATGATCGCAAGCGGTAATCATGAGCGTGACTGGCCAGGCTCAGGATCCTTCTATGGGACCACGGATTCAGGCGGGGAATGCGGTGTCTTGGCCCAGACCATGTTTTATGTCCCTGCTGAAAACAGGGCTAAGTTCTGGTATTCCACTGACTATGGCATGTTCCGGTTCTGCATTGCGGACACAGAACACGATTGGAGGGAGGGAACAGAGCAATACAAGTTCATTGAGGAGTGCCTAGCATCAGTCGATAGACAAAAGCAACCATGGCTGATCTTTCTGGCTCATCGGGTGCTAGGTTATTCTTCTGCAAGCTTTTATGTCGCCGAAGGATCATTTGAGGAACCAATGGGAAGGGAAAGCCTTCAGAAATTATGGCAGAAGTACAAAGTTGACCTCGCTGTATACGGCCATGTGCACAACTACGAAAGGAGTTGCCCCATTTATCAGAATATCTGCACCAGTAAGGAGCAGCACAATTACAAGGGTAACCTGAATGGCACGATACATGTGGTTGCTGGTGGCGGAGGAGCGAGTCTTGCAACCTTTGCTCCTGTTCAAACGGAATGGAGTATCTTCAAGGACTATGATTACGGGTTTATCAAACTGACAGCGTTTGATCGCTCGAACCTAATGTTCGAGTACAAGAAGAGCAGGGACGGAAAGGTCTACGATTCGTTCAGAATATCAAGGGAGTACAAGGACATCTTGGCCTGCTCTGTTGATAGTTGCCCGAGTACAACTCTGGCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

390

Amino Acids

44.22

Weight (kDa)

5.9

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1079
AccB7I CCANNNNNTGG 1 cut(s) 173
AccI GTMKAC 2 cut(s) 819, 1083
AccII CGCG 1 cut(s) 195
AciI CCGC 5 cut(s) 429, 489, 498, 593, 939
AclWI GGATC 6 cut(s) 32, 231, 244, 456, 469, 754
AcoI YGGCCR 2 cut(s) 449, 823
AcsI RAATTY 1 cut(s) 181
AcuI CTGAAG 2 cut(s) 86, 764
AfaI GTAC 5 cut(s) 92, 800, 1058, 1112, 1153
AfiI CCNNNNNNNGG 5 cut(s) 173, 331, 379, 458, 471
AflIII ACRYGT 2 cut(s) 193, 922
AgsI TTSAA 3 cut(s) 191, 974, 994
AjnI CCWGG 3 cut(s) 40, 172, 451
AluBI AGCT 3 cut(s) 230, 289, 729
AluI AGCT 3 cut(s) 230, 289, 729
Alw21I GWGCWC 2 cut(s) 13, 834
Alw44I GTGCAC 2 cut(s) 9, 830
AlwI GGATC 6 cut(s) 32, 231, 244, 456, 469, 754
Ama87I CYCGRG 1 cut(s) 1147
AoxI GGCC 5 cut(s) 77, 449, 507, 823, 1125
ApaLI GTGCAC 2 cut(s) 9, 830
ApeKI GCWGC 2 cut(s) 286, 886
ApoI RAATTY 1 cut(s) 181
Asp700I GAANNNNTTC 1 cut(s) 777
AspS9I GGNCC 4 cut(s) 78, 370, 474, 508
AsuHPI GGTGA 1 cut(s) 221
AvaI CYCGRG 1 cut(s) 1147
AvaII GGWCC 2 cut(s) 370, 474
BaeGI GKGCMC 2 cut(s) 13, 834
BalI TGGCCA 1 cut(s) 451
BamHI GGATCC 2 cut(s) 236, 461
Bbv12I GWGCWC 2 cut(s) 13, 834
BbvI GCAGC 2 cut(s) 298, 898
BccI CCATC 1 cut(s) 227
BceAI ACGGC 2 cut(s) 396, 838
BciT130I CCWGG 3 cut(s) 42, 174, 453
BciVI GTATCC 1 cut(s) 347
BfaI CTAG 2 cut(s) 653, 710
BfuI GTATCC 1 cut(s) 347
BisI GCNGC 2 cut(s) 287, 887
BlsI GCNGC 2 cut(s) 288, 888
Bme1390I CCNGG 3 cut(s) 42, 174, 453
Bme18I GGWCC 2 cut(s) 370, 474
BmeT110I CYCGRG 1 cut(s) 1147
BmgT120I GGNCC 4 cut(s) 78, 370, 474, 508
BmiI GGNNCC 6 cut(s) 127, 238, 371, 463, 475, 759
BmrFI CCNGG 3 cut(s) 42, 174, 453
BmsI GCATC 3 cut(s) 170, 410, 665
BplI GAGNNNNNCTC 1 cut(s) 1141
Bpu10I CCTNAGC 1 cut(s) 457
BsaJI CCNNGG 2 cut(s) 477, 680
BsaWI WCCGGW 1 cut(s) 579
BsaXI ACNNNNNCTCC 2 cut(s) 949, 979
Bsc4I CCNNNNNNNGG 5 cut(s) 173, 331, 379, 458, 471
Bse1I ACTGG 2 cut(s) 452, 876
Bse3DI GCAATG 1 cut(s) 588
BseBI CCWGG 3 cut(s) 42, 174, 453
BseDI CCNNGG 2 cut(s) 477, 680
BseGI GGATG 1 cut(s) 229
BseLI CCNNNNNNNGG 5 cut(s) 173, 331, 379, 458, 471
BseMI GCAATG 1 cut(s) 588
BseMII CTCAG 1 cut(s) 471
BseNI ACTGG 2 cut(s) 452, 876
BseRI GAGGAG 2 cut(s) 659, 957
BseSI GKGCMC 2 cut(s) 13, 834
BseXI GCAGC 2 cut(s) 298, 898
BsgI GTGCAG 1 cut(s) 856
Bsh1236I CGCG 1 cut(s) 195
BshFI GGCC 5 cut(s) 79, 451, 509, 825, 1127
BsiHKAI GWGCWC 2 cut(s) 13, 834
BsiHKCI CYCGRG 1 cut(s) 1147
BsiSI CCGG 1 cut(s) 580
BslFI GGGAC 4 cut(s) 383, 487, 512, 1085
BslI CCNNNNNNNGG 5 cut(s) 173, 331, 379, 458, 471
BsmFI GGGAC 4 cut(s) 383, 487, 512, 1085
BsmI GAATGC 1 cut(s) 500
BsnI GGCC 5 cut(s) 79, 451, 509, 825, 1127
BsoBI CYCGRG 1 cut(s) 1147
Bsp1286I GDGCHC 2 cut(s) 13, 834
Bsp1407I TGTACA 1 cut(s) 90
Bsp143I GATC 7 cut(s) 37, 236, 420, 461, 687, 746, 1033
Bsp19I CCATGG 1 cut(s) 680
BspACI CCGC 5 cut(s) 429, 489, 498, 593, 939
BspANI GGCC 5 cut(s) 79, 451, 509, 825, 1127
BspCNI CTCAG 1 cut(s) 470
BspFNI CGCG 1 cut(s) 195
BspHI TCATGA 1 cut(s) 436
BspLI GGNNCC 6 cut(s) 127, 238, 371, 463, 475, 759
BspPI GGATC 6 cut(s) 32, 231, 244, 456, 469, 754
BspQI GCTCTTC 1 cut(s) 1058
BsrDI GCAATG 1 cut(s) 588
BsrGI TGTACA 1 cut(s) 90
BsrI ACTGG 2 cut(s) 452, 876
BssECI CCNNGG 2 cut(s) 477, 680
BssMI GATC 7 cut(s) 37, 236, 420, 461, 687, 746, 1033
BssNAI GTATAC 1 cut(s) 820
BssT1I CCWWGG 1 cut(s) 680
Bst1107I GTATAC 1 cut(s) 820
Bst2UI CCWGG 3 cut(s) 42, 174, 453
Bst4CI ACNGT 2 cut(s) 25, 88
Bst6I CTCTTC 1 cut(s) 1058
BstAPI GCANNNNNTGC 1 cut(s) 962
BstAUI TGTACA 1 cut(s) 90
BstC8I GCNNGC 3 cut(s) 427, 727, 1129
BstDEI CTNAG 4 cut(s) 282, 301, 457, 546
BstDSI CCRYGG 2 cut(s) 477, 680
BstEII GGTNACC 1 cut(s) 902
BstF5I GGATG 1 cut(s) 229
BstFNI CGCG 1 cut(s) 195
BstKTI GATC 7 cut(s) 40, 239, 423, 464, 690, 749, 1036
BstMBI GATC 7 cut(s) 37, 236, 420, 461, 687, 746, 1033
BstMWI GCNNNNNNNGC 5 cut(s) 448, 495, 682, 953, 962
BstNI CCWGG 3 cut(s) 42, 174, 453
BstNSI RCATGY 2 cut(s) 577, 926
BstPI GGTNACC 1 cut(s) 902
BstSCI CCNGG 3 cut(s) 40, 172, 451
BstSLI GKGCMC 2 cut(s) 13, 834
BstUI CGCG 1 cut(s) 195
BstV1I GCAGC 2 cut(s) 298, 898
BstX2I RGATCY 3 cut(s) 37, 236, 461
BstYI RGATCY 3 cut(s) 37, 236, 461
BstZ17I GTATAC 1 cut(s) 820
BsuI GTATCC 1 cut(s) 347
BsuRI GGCC 5 cut(s) 79, 451, 509, 825, 1127
BtgI CCRYGG 2 cut(s) 477, 680
BtsCI GGATG 1 cut(s) 229
BtsI GCAGTG 2 cut(s) 54, 371
BtsIMutI CAGTG 5 cut(s) 30, 54, 93, 371, 561
Cac8I GCNNGC 3 cut(s) 427, 727, 1129
CciI TCATGA 1 cut(s) 436
Cfr13I GGNCC 4 cut(s) 78, 370, 474, 508
Csp6I GTAC 5 cut(s) 91, 799, 1057, 1111, 1152
CspCI CAANNNNNGTGG 2 cut(s) 6, 41
CviAII CATG 8 cut(s) 214, 418, 437, 517, 574, 681, 827, 923
CviQI GTAC 5 cut(s) 91, 799, 1057, 1111, 1152
DdeI CTNAG 4 cut(s) 282, 301, 457, 546
DpnI GATC 7 cut(s) 39, 238, 422, 463, 689, 748, 1035
DpnII GATC 7 cut(s) 37, 236, 420, 461, 687, 746, 1033
DrdI GACNNNNNNGTC 1 cut(s) 1079
DseDI GACNNNNNNGTC 1 cut(s) 1079
EaeI YGGCCR 2 cut(s) 449, 823
Eam1104I CTCTTC 1 cut(s) 1058
EarI CTCTTC 1 cut(s) 1058
EciI GGCGGA 1 cut(s) 954
Eco130I CCWWGG 1 cut(s) 680
Eco32I GATATC 1 cut(s) 316
Eco47I GGWCC 2 cut(s) 370, 474
Eco57I CTGAAG 2 cut(s) 86, 764
Eco88I CYCGRG 1 cut(s) 1147
Eco91I GGTNACC 1 cut(s) 902
EcoO65I GGTNACC 1 cut(s) 902
EcoRII CCWGG 3 cut(s) 40, 172, 451
EcoRV GATATC 1 cut(s) 316
EcoT14I CCWWGG 1 cut(s) 680
ErhI CCWWGG 1 cut(s) 680
FaeI CATG 8 cut(s) 217, 421, 440, 520, 577, 684, 830, 926
FalI AAGNNNNNCTT 2 cut(s) 1106, 1138
FaqI GGGAC 4 cut(s) 383, 487, 512, 1085
FatI CATG 8 cut(s) 213, 417, 436, 516, 573, 680, 826, 922
FauI CCCGC 1 cut(s) 482
FblI GTMKAC 2 cut(s) 819, 1083
Fnu4HI GCNGC 2 cut(s) 287, 887
FokI GGATG 1 cut(s) 236
Fsp4HI GCNGC 2 cut(s) 287, 887
FspBI CTAG 2 cut(s) 653, 710
GluI GCNGC 2 cut(s) 287, 887
HaeIII GGCC 5 cut(s) 79, 451, 509, 825, 1127
HapII CCGG 1 cut(s) 580
Hin1II CATG 8 cut(s) 217, 421, 440, 520, 577, 684, 830, 926
HincII GTYRAC 1 cut(s) 808
HindII GTYRAC 1 cut(s) 808
HindIII AAGCTT 1 cut(s) 727
HinfI GANTC 4 cut(s) 139, 482, 949, 1088
HpaII CCGG 1 cut(s) 580
HphI GGTGA 1 cut(s) 221
Hpy166II GTNNAC 7 cut(s) 11, 91, 93, 808, 820, 832, 1084
Hpy188I TCNGA 5 cut(s) 66, 158, 783, 864, 1097
Hpy188III TCNNGA 4 cut(s) 143, 437, 459, 1167
Hpy8I GTNNAC 7 cut(s) 11, 91, 93, 808, 820, 832, 1084
HpyAV CCTTC 5 cut(s) 61, 475, 737, 762, 788
HpyCH4III ACNGT 2 cut(s) 25, 88
HpyCH4V TGCA 6 cut(s) 11, 588, 725, 832, 873, 956
HpyF10VI GCNNNNNNNGC 5 cut(s) 448, 495, 682, 953, 962
HpyF3I CTNAG 4 cut(s) 282, 301, 457, 546
Hsp92II CATG 8 cut(s) 217, 421, 440, 520, 577, 684, 830, 926
Kzo9I GATC 7 cut(s) 37, 236, 420, 461, 687, 746, 1033
LguI GCTCTTC 1 cut(s) 1058
LmnI GCTCC 3 cut(s) 883, 944, 970
Lsp1109I GCAGC 2 cut(s) 298, 898
LweI GCATC 3 cut(s) 170, 410, 665
MaeI CTAG 2 cut(s) 653, 710
MaeIII GTNAC 4 cut(s) 209, 341, 443, 902
MalI GATC 7 cut(s) 39, 238, 422, 463, 689, 748, 1035
MboI GATC 7 cut(s) 37, 236, 420, 461, 687, 746, 1033
MboII GAAGA 4 cut(s) 196, 711, 982, 1075
MflI RGATCY 3 cut(s) 37, 236, 461
MhlI GDGCHC 2 cut(s) 13, 834
MlsI TGGCCA 1 cut(s) 451
MluCI AATT 6 cut(s) 71, 181, 309, 374, 785, 892
MluI ACGCGT 1 cut(s) 193
MluNI TGGCCA 1 cut(s) 451
MlyI GAGTC 2 cut(s) 148, 958
MmeI TCCRAC 1 cut(s) 85
MnlI CCTC 6 cut(s) 606, 610, 637, 748, 821, 935
Mox20I TGGCCA 1 cut(s) 451
MroXI GAANNNNTTC 1 cut(s) 777
MscI TGGCCA 1 cut(s) 451
MslI CAYNNNNRTG 1 cut(s) 441
Msp20I TGGCCA 1 cut(s) 451
MspI CCGG 1 cut(s) 580
MspR9I CCNGG 3 cut(s) 42, 174, 453
Mva1269I GAATGC 1 cut(s) 500
MvaI CCWGG 3 cut(s) 42, 174, 453
MvnI CGCG 1 cut(s) 195
MwoI GCNNNNNNNGC 5 cut(s) 448, 495, 682, 953, 962
NcoI CCATGG 1 cut(s) 680
NdeII GATC 7 cut(s) 37, 236, 420, 461, 687, 746, 1033
NlaIII CATG 8 cut(s) 217, 421, 440, 520, 577, 684, 830, 926
NlaIV GGNNCC 6 cut(s) 127, 238, 371, 463, 475, 759
NmuCI GTSAC 2 cut(s) 209, 443
NspI RCATGY 2 cut(s) 577, 926
PagI TCATGA 1 cut(s) 436
PciI ACATGT 1 cut(s) 922
PciSI GCTCTTC 1 cut(s) 1058
PctI GAATGC 1 cut(s) 500
PdmI GAANNNNTTC 1 cut(s) 777
PfeI GAWTC 2 cut(s) 482, 1088
PflMI CCANNNNNTGG 1 cut(s) 173
PfoI TCCNGGA 1 cut(s) 40
PkrI GCNGC 2 cut(s) 288, 888
PleI GAGTC 2 cut(s) 147, 957
PpsI GAGTC 2 cut(s) 147, 957
PscI ACATGT 1 cut(s) 922
Psp6I CCWGG 3 cut(s) 40, 172, 451
PspEI GGTNACC 1 cut(s) 902
PspGI CCWGG 3 cut(s) 40, 172, 451
PspN4I GGNNCC 6 cut(s) 127, 238, 371, 463, 475, 759
PspPI GGNCC 4 cut(s) 78, 370, 474, 508
PsuI RGATCY 3 cut(s) 37, 236, 461
RsaI GTAC 5 cut(s) 92, 800, 1058, 1112, 1153
RsaNI GTAC 5 cut(s) 91, 799, 1057, 1111, 1152
RseI CAYNNNNRTG 1 cut(s) 441
SapI GCTCTTC 1 cut(s) 1058
SatI GCNGC 2 cut(s) 287, 887
Sau3AI GATC 7 cut(s) 37, 236, 420, 461, 687, 746, 1033
Sau96I GGNCC 4 cut(s) 78, 370, 474, 508
SchI GAGTC 2 cut(s) 148, 958
ScrFI CCNGG 3 cut(s) 42, 174, 453
SduI GDGCHC 2 cut(s) 13, 834
SfaNI GCATC 3 cut(s) 170, 410, 665
SinI GGWCC 2 cut(s) 370, 474
SmiMI CAYNNNNRTG 1 cut(s) 441
Sse9I AATT 6 cut(s) 71, 181, 309, 374, 785, 892
SsiI CCGC 5 cut(s) 429, 489, 498, 593, 939
SspMI CTAG 2 cut(s) 653, 710
StyD4I CCNGG 3 cut(s) 40, 172, 451
StyI CCWWGG 1 cut(s) 680
TaaI ACNGT 2 cut(s) 25, 88
TaqI TCGA 3 cut(s) 663, 1040, 1053
TasI AATT 6 cut(s) 71, 181, 309, 374, 785, 892
TatI WGTACW 5 cut(s) 90, 798, 1056, 1110, 1151
TfiI GAWTC 2 cut(s) 482, 1088
TscAI CASTG 5 cut(s) 30, 61, 93, 371, 568
TseFI GTSAC 2 cut(s) 209, 443
TseI GCWGC 2 cut(s) 286, 886
Tsp45I GTSAC 2 cut(s) 209, 443
TspDTI ATGAA 3 cut(s) 240, 258, 628
TspGWI ACGGA 3 cut(s) 494, 992, 1089
TspRI CASTG 5 cut(s) 30, 61, 93, 371, 568
Van91I CCANNNNNTGG 1 cut(s) 173
VneI GTGCAC 2 cut(s) 9, 830
VpaK11BI GGWCC 2 cut(s) 370, 474
XapI RAATTY 1 cut(s) 181
XceI RCATGY 2 cut(s) 577, 926
XcmI CCANNNNNNNNNTGG 1 cut(s) 260
XmiI GTMKAC 2 cut(s) 819, 1083
XmnI GAANNNNTTC 1 cut(s) 777
XspI CTAG 2 cut(s) 653, 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.