MD16G1061100.v1.1

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002060
Physical Location & Seq
Forward (+)
4365590 .. 4369718
4129 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1061100.v1.1.491

Sequence Viewer

Length: 1872 bp
ATGGGATTTAATACCCTAGGCAGGATGAGAAGGTTGAAATATTTCTGCTTGGCAGTTCATTTGGTTCTTCTTGCAACTCTTCAAGTTCAAGATGTGGAGTCTCATGGAGATCAGCCTTTGTCAAAGATTGCTGTTCACAAGGCAGTGTCTTCCCTGCATTCCCATGCTTATGTGAAGGCCTCCTCTGCAGTTCTTGGGCTCCAGGGGCAATATGTGGAATGGTTGACATTGGAGTTCAGTTCTCCACACCCTTCAAATGATGACTGGATTGGAGTGTTTTCTCCTGCCAATTTCAGTGCTTCGACCTGCCCTGCGGAAACTAAAAGCTCTTCTGCTCCATTTTTATGTTCAGCACCTATAAAGTACCAGTATGCGAACTACACTAGTCCTAGGTACAAAGATACCGGGAAAGGCTCCTTGAAACTGCAGTTGATTAACCAGAGATCAGATTTCGCTTTTGCACTATTCTCGGGTGGTTTATCAGAGCCGAAGCTGGTGGCAGTGTCAAATAAAGTTGCATTCGCAAATCCGAAGGCTCCAGTTTATCCACGCCTAGCGCAAGGAAAATTGTGGAATGAAATGACTGTAACATGGACAAGTGGATATGACATCGTGGAAGCAACCCCTTTTGTTGAGTGGGGTATAAAAGGAGAAGACCGTGTGAAATCCCCAGCTGGGACATTGAACTTCGACCGCAACACCATGTGTGGTGCACCGGCAAAGACCGTGGGGTGGCGAGATCCTGGATTTATACACACTGCTTTTCTGAAGGAATTGTGGCCCAACACAGTGTACACCTACAAGGTTGGACACAGATTGCCTAATGGTTCCCATATTTTGAGTCAAGAATACAAGTTCAGAGCATCTCCATATCCTGGTCAAAATTCTGTTCAACGCGTTGTCATCTTCGGTGACATGGGAAAGGATGAAGCTGATGGATCCAATGAATATAACCAATACCAGCGTGGCTCTCTCAACACTACTAAGCAGCTAATCCAAGACTTAGAGAAAATTGATATCGTTTTCCACATTGGAGATATATGTTACGCAAATGGATACCTTTCGCAGTGGGATCAATTCACAGCACAGGTTGAGCCGATAGCATCTGCTGTTCCTTACATGATCGCAAGCGGTAATCATGAGCGTGACTGGCCAGGCTCAGGATCCTTCTATGGGAACATGGATTCAGGCGGGGAATGCGGTGTCTTGGCCCAGACCATGTTTTATGTCCCTGCTGAAAACAGGGCTAAGTTCTGGTATTCCACTGACTATGGCATGTTCCGGTTCTGCATTGCGGACACAGAACATGATTGGAGAGAGGGAACAGAGCAATACAAGTTCATTGAGGAGTGCCTAGCATCAGTCGATAGACAAAAGCAACCATGGCTGATCTTTCTGGCTCATCGGGTGCTAGGTTATTCTTCTGCAAGCTTTTATGTCGCCGAAGGATCGTTTGAGGAACCAATGGGAAGGGAAAGCCTTCAGAAATTATGGCAGAAGTACAAAGTTGACCTCGCCGTATACGGCCATGTGCACAACTACGAAAGGAGTTGCCCCATTTATCAGAATATCTGCACCAGTAAGGAGAAGCACAATTACAAGGGTAACTTGAATGGCACGATACATGTGGTTGCTGGTGGCGGAGGAGCGAGTCTTGCAACCTTTGCTCCCGTTCAAACGGAATGGAGTATCTTCAAGGACTATGATTACGGGTTTGTCAAACTTACAGCGTTTGATCGCTCGAACCTATTGTTCGAGTACAAGAAGAGCAGGGACGGAAAGGTCTATGATTCGTTCAGAATATCAAGGGAGTACAAGGACATCTTGGCCTGCTCTGTTGATAGTTGCCCGAGTACAACTCTGGCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

624

Amino Acids

69.92

Weight (kDa)

6.98

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_PAP PF17808 58 - 176 3.8e-44 Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N PF16656 184 - 287 3e-12 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 299 - 514 2.7e-18 Calcineurin-like phosphoesterase
Metallophos_C PF14008 539 - 598 3.6e-16 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1781
Acc36I ACCTGC 1 cut(s) 314
AccB7I CCANNNNNTGG 1 cut(s) 875
AccI GTMKAC 1 cut(s) 1521
AccII CGCG 1 cut(s) 897
AciI CCGC 7 cut(s) 314, 694, 1131, 1191, 1200, 1295, 1641
AclWI GGATC 7 cut(s) 734, 933, 946, 1080, 1158, 1171, 1456
AcoI YGGCCR 2 cut(s) 1151, 1525
AcsI RAATTY 1 cut(s) 883
AcuI CTGAAG 2 cut(s) 788, 1466
AdeI CACNNNGTG 1 cut(s) 705
AfaI GTAC 7 cut(s) 365, 395, 794, 1502, 1760, 1814, 1855
AfiI CCNNNNNNNGG 6 cut(s) 21, 675, 732, 875, 1160, 1173
AflIII ACRYGT 2 cut(s) 895, 1624
AhlI ACTAGT 1 cut(s) 383
AjnI CCWGG 4 cut(s) 201, 742, 874, 1153
AluBI AGCT 6 cut(s) 327, 493, 674, 932, 991, 1431
AluI AGCT 6 cut(s) 327, 493, 674, 932, 991, 1431
Alw21I GWGCWC 2 cut(s) 715, 1536
Alw26I GTCTC 1 cut(s) 105
Alw44I GTGCAC 2 cut(s) 711, 1532
AlwI GGATC 7 cut(s) 734, 933, 946, 1080, 1158, 1171, 1456
Ama87I CYCGRG 2 cut(s) 469, 1849
AoxI GGCC 6 cut(s) 177, 779, 1151, 1209, 1525, 1827
ApaLI GTGCAC 2 cut(s) 711, 1532
ApeKI GCWGC 1 cut(s) 988
ApoI RAATTY 1 cut(s) 883
Asp700I GAANNNNTTC 2 cut(s) 41, 1479
AspA2I CCTAGG 2 cut(s) 16, 389
AspLEI GCGC 1 cut(s) 559
AspS9I GGNCC 2 cut(s) 780, 1210
AsuC2I CCSGG 1 cut(s) 406
AsuHPI GGTGA 1 cut(s) 923
AvaI CYCGRG 2 cut(s) 469, 1849
AvrII CCTAGG 2 cut(s) 16, 389
BaeGI GKGCMC 2 cut(s) 715, 1536
BalI TGGCCA 1 cut(s) 1153
BamHI GGATCC 2 cut(s) 938, 1163
BanII GRGCYC 1 cut(s) 201
BarI GAAGNNNNNNTAC 2 cut(s) 1580, 1612
BbsI GAAGAC 2 cut(s) 141, 660
Bbv12I GWGCWC 2 cut(s) 715, 1536
BbvI GCAGC 1 cut(s) 1000
BccI CCATC 1 cut(s) 929
BceAI ACGGC 2 cut(s) 1502, 1540
BcgI CGANNNNNNTGC 2 cut(s) 450, 484
BciT130I CCWGG 4 cut(s) 203, 744, 876, 1155
BciVI GTATCC 1 cut(s) 1049
BcnI CCSGG 1 cut(s) 406
BcoDI GTCTC 1 cut(s) 105
BcuI ACTAGT 1 cut(s) 383
BfaI CTAG 6 cut(s) 17, 384, 390, 554, 1355, 1412
BfmI CTRYAG 2 cut(s) 186, 425
BfuAI ACCTGC 1 cut(s) 314
BfuI GTATCC 1 cut(s) 1049
BisI GCNGC 1 cut(s) 989
BlnI CCTAGG 2 cut(s) 16, 389
BlsI GCNGC 1 cut(s) 990
Bme1390I CCNGG 5 cut(s) 203, 406, 744, 876, 1155
BmeT110I CYCGRG 2 cut(s) 469, 1849
BmgT120I GGNCC 2 cut(s) 780, 1210
BmiI GGNNCC 7 cut(s) 200, 415, 537, 829, 940, 1165, 1461
BmrFI CCNGG 5 cut(s) 203, 406, 744, 876, 1155
BmsI GCATC 3 cut(s) 872, 1112, 1367
BpiI GAAGAC 2 cut(s) 141, 660
BplI GAGNNNNNCTC 1 cut(s) 1843
BpmI CTGGAG 2 cut(s) 185, 522
Bpu10I CCTNAGC 1 cut(s) 1159
BpuMI CCSGG 1 cut(s) 406
BsaJI CCNNGG 5 cut(s) 16, 202, 389, 726, 1382
BsaWI WCCGGW 1 cut(s) 1281
BsaXI ACNNNNNCTCC 2 cut(s) 1651, 1681
Bsc4I CCNNNNNNNGG 6 cut(s) 21, 675, 732, 875, 1160, 1173
Bse118I RCCGGY 1 cut(s) 715
Bse1I ACTGG 5 cut(s) 269, 367, 539, 1154, 1578
Bse3DI GCAATG 1 cut(s) 1290
BseBI CCWGG 4 cut(s) 203, 744, 876, 1155
BseDI CCNNGG 5 cut(s) 16, 202, 389, 726, 1382
BseGI GGATG 2 cut(s) 30, 931
BseLI CCNNNNNNNGG 6 cut(s) 21, 675, 732, 875, 1160, 1173
BseMI GCAATG 1 cut(s) 1290
BseMII CTCAG 1 cut(s) 1173
BseNI ACTGG 5 cut(s) 269, 367, 539, 1154, 1578
BseRI GAGGAG 3 cut(s) 172, 1361, 1659
BseSI GKGCMC 2 cut(s) 715, 1536
BseXI GCAGC 1 cut(s) 1000
BseYI CCCAGC 2 cut(s) 670, 674
BsgI GTGCAG 1 cut(s) 1558
Bsh1236I CGCG 1 cut(s) 897
Bsh1285I CGRYCG 1 cut(s) 694
BshFI GGCC 6 cut(s) 179, 781, 1153, 1211, 1527, 1829
BsiEI CGRYCG 1 cut(s) 694
BsiHKAI GWGCWC 2 cut(s) 715, 1536
BsiHKCI CYCGRG 2 cut(s) 469, 1849
BsiSI CCGG 3 cut(s) 405, 716, 1282
BslFI GGGAC 3 cut(s) 691, 1214, 1787
BslI CCNNNNNNNGG 6 cut(s) 21, 675, 732, 875, 1160, 1173
BsmAI GTCTC 1 cut(s) 105
BsmFI GGGAC 3 cut(s) 691, 1214, 1787
BsmI GAATGC 3 cut(s) 157, 518, 1202
BsnI GGCC 6 cut(s) 179, 781, 1153, 1211, 1527, 1829
BsoBI CYCGRG 2 cut(s) 469, 1849
Bsp1286I GDGCHC 3 cut(s) 201, 715, 1536
Bsp1407I TGTACA 1 cut(s) 792
Bsp19I CCATGG 1 cut(s) 1382
BspACI CCGC 7 cut(s) 314, 694, 1131, 1191, 1200, 1295, 1641
BspANI GGCC 6 cut(s) 179, 781, 1153, 1211, 1527, 1829
BspCNI CTCAG 1 cut(s) 1172
BspFNI CGCG 1 cut(s) 897
BspHI TCATGA 1 cut(s) 1138
BspLI GGNNCC 7 cut(s) 200, 415, 537, 829, 940, 1165, 1461
BspMAI CTGCAG 2 cut(s) 190, 429
BspMI ACCTGC 1 cut(s) 314
BspPI GGATC 7 cut(s) 734, 933, 946, 1080, 1158, 1171, 1456
BspQI GCTCTTC 2 cut(s) 334, 1760
BsrDI GCAATG 1 cut(s) 1290
BsrFI RCCGGY 1 cut(s) 715
BsrGI TGTACA 1 cut(s) 792
BsrI ACTGG 5 cut(s) 269, 367, 539, 1154, 1578
BssAI RCCGGY 1 cut(s) 715
BssECI CCNNGG 5 cut(s) 16, 202, 389, 726, 1382
BssNAI GTATAC 1 cut(s) 1522
BssT1I CCWWGG 3 cut(s) 16, 389, 1382
Bst1107I GTATAC 1 cut(s) 1522
Bst2UI CCWGG 4 cut(s) 203, 744, 876, 1155
Bst4CI ACNGT 4 cut(s) 586, 659, 727, 790
Bst6I CTCTTC 3 cut(s) 84, 334, 1760
BstAPI GCANNNNNTGC 1 cut(s) 1664
BstAUI TGTACA 1 cut(s) 792
BstC8I GCNNGC 3 cut(s) 1129, 1429, 1831
BstDEI CTNAG 4 cut(s) 984, 1003, 1159, 1248
BstDSI CCRYGG 2 cut(s) 726, 1382
BstF5I GGATG 2 cut(s) 30, 931
BstFNI CGCG 1 cut(s) 897
BstHHI GCGC 1 cut(s) 559
BstMAI GTCTC 1 cut(s) 105
BstMCI CGRYCG 1 cut(s) 694
BstMWI GCNNNNNNNGC 7 cut(s) 185, 205, 1150, 1197, 1384, 1655, 1664
BstNI CCWGG 4 cut(s) 203, 744, 876, 1155
BstNSI RCATGY 2 cut(s) 1279, 1628
BstSCI CCNGG 5 cut(s) 201, 404, 742, 874, 1153
BstSFI CTRYAG 2 cut(s) 186, 425
BstSLI GKGCMC 2 cut(s) 715, 1536
BstUI CGCG 1 cut(s) 897
BstV1I GCAGC 1 cut(s) 1000
BstV2I GAAGAC 2 cut(s) 141, 660
BstX2I RGATCY 3 cut(s) 739, 938, 1163
BstYI RGATCY 3 cut(s) 739, 938, 1163
BstZ17I GTATAC 1 cut(s) 1522
BsuI GTATCC 1 cut(s) 1049
BsuRI GGCC 6 cut(s) 179, 781, 1153, 1211, 1527, 1829
BtgI CCRYGG 2 cut(s) 726, 1382
BtsCI GGATG 2 cut(s) 30, 931
BtsI GCAGTG 4 cut(s) 150, 507, 756, 1073
BtsIMutI CAGTG 7 cut(s) 150, 301, 507, 756, 795, 1073, 1263
BveI ACCTGC 1 cut(s) 314
Cac8I GCNNGC 3 cut(s) 1129, 1429, 1831
CciI TCATGA 1 cut(s) 1138
CfoI GCGC 1 cut(s) 559
Cfr10I RCCGGY 1 cut(s) 715
Cfr13I GGNCC 2 cut(s) 780, 1210
Csp6I GTAC 7 cut(s) 364, 394, 793, 1501, 1759, 1813, 1854
CspCI CAANNNNNGTGG 2 cut(s) 708, 743
CviQI GTAC 7 cut(s) 364, 394, 793, 1501, 1759, 1813, 1854
DdeI CTNAG 4 cut(s) 984, 1003, 1159, 1248
DraIII CACNNNGTG 1 cut(s) 705
DrdI GACNNNNNNGTC 1 cut(s) 1781
DseDI GACNNNNNNGTC 1 cut(s) 1781
EaeI YGGCCR 2 cut(s) 1151, 1525
Eam1104I CTCTTC 3 cut(s) 84, 334, 1760
EarI CTCTTC 3 cut(s) 84, 334, 1760
EciI GGCGGA 1 cut(s) 1656
Eco130I CCWWGG 3 cut(s) 16, 389, 1382
Eco147I AGGCCT 1 cut(s) 179
Eco24I GRGCYC 1 cut(s) 201
Eco32I GATATC 1 cut(s) 1018
Eco57I CTGAAG 2 cut(s) 788, 1466
Eco88I CYCGRG 2 cut(s) 469, 1849
EcoRII CCWGG 4 cut(s) 201, 742, 874, 1153
EcoRV GATATC 1 cut(s) 1018
EcoT14I CCWWGG 3 cut(s) 16, 389, 1382
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 3 cut(s) 16, 389, 1382
FalI AAGNNNNNCTT 4 cut(s) 1592, 1624, 1808, 1840
FaqI GGGAC 3 cut(s) 691, 1214, 1787
FauI CCCGC 1 cut(s) 1184
FblI GTMKAC 1 cut(s) 1521
Fnu4HI GCNGC 1 cut(s) 989
FokI GGATG 2 cut(s) 37, 938
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 1 cut(s) 989
FspBI CTAG 6 cut(s) 17, 384, 390, 554, 1355, 1412
GlaI GCGC 1 cut(s) 558
GluI GCNGC 1 cut(s) 989
GsaI CCCAGC 2 cut(s) 674, 678
GsuI CTGGAG 2 cut(s) 185, 522
HaeIII GGCC 6 cut(s) 179, 781, 1153, 1211, 1527, 1829
HapII CCGG 3 cut(s) 405, 716, 1282
HhaI GCGC 1 cut(s) 559
Hin6I GCGC 1 cut(s) 557
HinP1I GCGC 1 cut(s) 557
HincII GTYRAC 2 cut(s) 225, 1510
HindII GTYRAC 2 cut(s) 225, 1510
HindIII AAGCTT 1 cut(s) 1429
HinfI GANTC 5 cut(s) 98, 841, 1184, 1651, 1790
HpaII CCGG 3 cut(s) 405, 716, 1282
HphI GGTGA 1 cut(s) 923
Hpy166II GTNNAC 8 cut(s) 136, 225, 713, 793, 795, 1510, 1522, 1534
Hpy188I TCNGA 8 cut(s) 448, 484, 531, 768, 860, 1485, 1566, 1799
Hpy188III TCNNGA 5 cut(s) 89, 845, 1139, 1161, 1869
Hpy8I GTNNAC 8 cut(s) 136, 225, 713, 793, 795, 1510, 1522, 1534
HpyAV CCTTC 9 cut(s) 24, 169, 261, 526, 763, 1177, 1439, 1464, 1490
HpyCH4III ACNGT 4 cut(s) 586, 659, 727, 790
HpyF10VI GCNNNNNNNGC 7 cut(s) 185, 205, 1150, 1197, 1384, 1655, 1664
HpyF3I CTNAG 4 cut(s) 984, 1003, 1159, 1248
HspAI GCGC 1 cut(s) 557
LguI GCTCTTC 2 cut(s) 334, 1760
LmnI GCTCC 6 cut(s) 204, 340, 419, 541, 1646, 1672
Lsp1109I GCAGC 1 cut(s) 1000
LweI GCATC 3 cut(s) 872, 1112, 1367
MaeI CTAG 6 cut(s) 17, 384, 390, 554, 1355, 1412
MaeIII GTNAC 5 cut(s) 586, 911, 1043, 1145, 1604
MboII GAAGA 9 cut(s) 59, 71, 141, 321, 665, 898, 1413, 1684, 1777
MflI RGATCY 3 cut(s) 739, 938, 1163
MhlI GDGCHC 3 cut(s) 201, 715, 1536
MlsI TGGCCA 1 cut(s) 1153
MluCI AATT 8 cut(s) 289, 566, 773, 883, 1011, 1076, 1487, 1594
MluI ACGCGT 1 cut(s) 895
MluNI TGGCCA 1 cut(s) 1153
MlyI GAGTC 3 cut(s) 107, 850, 1660
MmeI TCCRAC 1 cut(s) 787
MnlI CCTC 7 cut(s) 190, 193, 1312, 1339, 1450, 1523, 1637
Mox20I TGGCCA 1 cut(s) 1153
MroXI GAANNNNTTC 2 cut(s) 41, 1479
MscI TGGCCA 1 cut(s) 1153
MseI TTAA 2 cut(s) 9, 435
MslI CAYNNNNRTG 4 cut(s) 162, 168, 343, 1143
Msp20I TGGCCA 1 cut(s) 1153
MspA1I CMGCKG 1 cut(s) 674
MspI CCGG 3 cut(s) 405, 716, 1282
MspR9I CCNGG 5 cut(s) 203, 406, 744, 876, 1155
Mva1269I GAATGC 3 cut(s) 157, 518, 1202
MvaI CCWGG 4 cut(s) 203, 744, 876, 1155
MvnI CGCG 1 cut(s) 897
MwoI GCNNNNNNNGC 7 cut(s) 185, 205, 1150, 1197, 1384, 1655, 1664
NciI CCSGG 1 cut(s) 406
NcoI CCATGG 1 cut(s) 1382
NlaIV GGNNCC 7 cut(s) 200, 415, 537, 829, 940, 1165, 1461
NmuCI GTSAC 2 cut(s) 911, 1145
NspI RCATGY 2 cut(s) 1279, 1628
PagI TCATGA 1 cut(s) 1138
PceI AGGCCT 1 cut(s) 179
PciI ACATGT 1 cut(s) 1624
PciSI GCTCTTC 2 cut(s) 334, 1760
PctI GAATGC 3 cut(s) 157, 518, 1202
PdmI GAANNNNTTC 2 cut(s) 41, 1479
PfeI GAWTC 2 cut(s) 1184, 1790
PflMI CCANNNNNTGG 1 cut(s) 875
PfoI TCCNGGA 1 cut(s) 742
PkrI GCNGC 1 cut(s) 990
PleI GAGTC 3 cut(s) 106, 849, 1659
PpsI GAGTC 3 cut(s) 106, 849, 1659
PscI ACATGT 1 cut(s) 1624
Psp6I CCWGG 4 cut(s) 201, 742, 874, 1153
PspFI CCCAGC 2 cut(s) 670, 674
PspGI CCWGG 4 cut(s) 201, 742, 874, 1153
PspN4I GGNNCC 7 cut(s) 200, 415, 537, 829, 940, 1165, 1461
PspPI GGNCC 2 cut(s) 780, 1210
PstI CTGCAG 2 cut(s) 190, 429
PsuI RGATCY 3 cut(s) 739, 938, 1163
PvuII CAGCTG 1 cut(s) 674
RsaI GTAC 7 cut(s) 365, 395, 794, 1502, 1760, 1814, 1855
RsaNI GTAC 7 cut(s) 364, 394, 793, 1501, 1759, 1813, 1854
RseI CAYNNNNRTG 4 cut(s) 162, 168, 343, 1143
SapI GCTCTTC 2 cut(s) 334, 1760
SaqAI TTAA 2 cut(s) 9, 435
SatI GCNGC 1 cut(s) 989
Sau96I GGNCC 2 cut(s) 780, 1210
SchI GAGTC 3 cut(s) 107, 850, 1660
ScrFI CCNGG 5 cut(s) 203, 406, 744, 876, 1155
SduI GDGCHC 3 cut(s) 201, 715, 1536
SfaNI GCATC 3 cut(s) 872, 1112, 1367
SfcI CTRYAG 2 cut(s) 186, 425
SmiMI CAYNNNNRTG 4 cut(s) 162, 168, 343, 1143
SpeI ACTAGT 1 cut(s) 383
Sse9I AATT 8 cut(s) 289, 566, 773, 883, 1011, 1076, 1487, 1594
SseBI AGGCCT 1 cut(s) 179
SsiI CCGC 7 cut(s) 314, 694, 1131, 1191, 1200, 1295, 1641
SspI AATATT 1 cut(s) 41
SspMI CTAG 6 cut(s) 17, 384, 390, 554, 1355, 1412
StuI AGGCCT 1 cut(s) 179
StyD4I CCNGG 5 cut(s) 201, 404, 742, 874, 1153
StyI CCWWGG 3 cut(s) 16, 389, 1382
TaaI ACNGT 4 cut(s) 586, 659, 727, 790
TaqI TCGA 5 cut(s) 302, 690, 1365, 1742, 1755
TasI AATT 8 cut(s) 289, 566, 773, 883, 1011, 1076, 1487, 1594
TatI WGTACW 5 cut(s) 792, 1500, 1758, 1812, 1853
TfiI GAWTC 2 cut(s) 1184, 1790
Tru1I TTAA 2 cut(s) 9, 435
Tru9I TTAA 2 cut(s) 9, 435
TscAI CASTG 7 cut(s) 150, 301, 507, 763, 795, 1073, 1270
TseFI GTSAC 2 cut(s) 911, 1145
TseI GCWGC 1 cut(s) 988
Tsp45I GTSAC 2 cut(s) 911, 1145
TspDTI ATGAA 5 cut(s) 47, 591, 942, 960, 1330
TspGWI ACGGA 2 cut(s) 1694, 1791
TspRI CASTG 7 cut(s) 150, 301, 507, 763, 795, 1073, 1270
Van91I CCANNNNNTGG 1 cut(s) 875
VneI GTGCAC 2 cut(s) 711, 1532
XapI RAATTY 1 cut(s) 883
XceI RCATGY 2 cut(s) 1279, 1628
XcmI CCANNNNNNNNNTGG 1 cut(s) 962
XmaJI CCTAGG 2 cut(s) 16, 389
XmiI GTMKAC 1 cut(s) 1521
XmnI GAANNNNTTC 2 cut(s) 41, 1479
XspI CTAG 6 cut(s) 17, 384, 390, 554, 1355, 1412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.