RchiOBHm_Chr4g0438291

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002060
Physical Location & Seq
Reverse (-)
61099566 .. 61103283
3718 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40643

Sequence Viewer

Length: 1887 bp
ATGTTAGCCTTCTTCTTCGGAGATTTAAAAATCTATAGATACTGTGTGGGGATGAAACAACTGAAAGTGATGAGGTTGTTAATTCTGTTGGGTCTTGTAATCATCCAAGAGGCAACTTCACATGGAGAGCACCCTCTTTCCAGAATTGCTATTCATGAAGCAACCTTTGCTCTTCATGACCTTGCTTATATCAAAGCCTCTCCTACTGTTCTAGGATTAAGGGGGCAAGACACTGAATGGGTGACTTTGGAATTTGGTTCTGAAAACCCTTCAACTGAGGATTGGATTGGAGTATTTTCTCCTGCTAATTTTAGTGCTTCTTCCTGCCCTGAGGAAAATCCAAGAGTTTATCCTCCATTTTTGTGCTCTGCACCTATTAAGTTTCAATATGCAAATTACTCCAGTCCCGAGTACAAGGACACTGGAAAAGGCTTCTTGAAGCTTCAGCTGATCAACCAGAGATCAGACTTCTCTTTCGCGCTGTTCTCTGGTGGTTTACTGAATCCAAAGGTCGTGGCTGTGTCAAATGTTGTAGCTTTCTCTAATCCAAATGCACCAGTTTATCCACGAGTAGCACAAGGGAAAGAATGGAATGAAATGACTGTAACATGGACTAGTGGATATGGGATTGATGTGGCAGAGCCTTTTGTTGATTGGGGTCCAAGAGGAGAACGTTTGCGTTCCCCAGCTGTGACACAAACTTTTGATCGTCATAGCATGTGTGGTGCACCTGCAAGAACAGTGGGATGGCGTGACCCTGGATTCATACATACTAGTTTTCTGAAGGAGTTGTGGCCAAATAAAGTGTATACGTACAGGCTGGGACATAGATTGTCTAATGGTACATATATATGGAGTCAAGACTATCAGTTTAAAGCATCTCCTTATCCTGGTCAAAATTCTTTACAACGTGTAGTCATTTTCGGTGACATGGGAAAGGATGAAGCTGATGGTTCCAATGAATATAACAATTTCCAGAGAGGCTCTCTCAACACTACTAAGCAGCTTATTCGAGACCTGAAAAACATTGATATTGTGTTCCATATTGGAGATATATGTTATGCAAATGGTTACCTTTCACAATGGGACCAATTTACTGCACAAGTTGAGCCAATAGCATCAACTGTTCCTTACATGATTGCAAGTGGCAACCATGAACGCGACTGGCCAGGATCAGGTTCTTTCTATGGGAACATGGATTCAGGAGGAGAATGTGGTGTGTTGGCTGAAAATATGTTCTATGTTCCCACCAAGAACAATGCTAAGTTCTGGTACTCCACTGACTATGGCATGTTTCACTTCTGCATAGCGGACACAGAACATGATTGGAGAGAGGGAACAGAGCAGTACCAATTCATTGAGCACTGCTTAGCATCAGTTGATAGACAGAAGCAACCATGGCTAATTTTTCTTGCACATCGGGTTCTCGGATATTCTTCTTGTGTCTCTTATGCAGAAGAAGGATCATTTAATGAACCAATGGGGAGGGAGAGCCTTCAGAAGCTATGGCAAAAGTACAAGGTTGACATTGCCATTTATGGTCATGTACATAACTATGAAAGAACATGTCCCATATACCAGAATATTTGCACAAATGAAGAGAAGCATTATTATAAGGGCAGCTTGAATGGAACAATACATGTGGTTGCTGGCGGTGCAGGAGCAAGTCTATCAACTTTTACATCCCTTCAAACAAAATGGAGTATTTTCAAAGACTATGACCATGGATTTGTCAAGCTCACAGCATTTGATCATTCAAATTTGTTGTTCGAGTACAAGAAGAGCAGGGACGGACAGGTTTATGACTATTTCAGAATATCCAGAGATTATAGGGACATCTTGGCCTGCACGGTTGATAGTTGTCCAAGCACGACACTAGCGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

628

Amino Acids

71.28

Weight (kDa)

6.11

Isoelectric Point (pI)

34.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_PAP PF17808 64 - 182 1.5e-42 Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N PF16656 190 - 292 2.7e-11 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 304 - 519 2.9e-18 Calcineurin-like phosphoesterase
Metallophos_C PF14008 544 - 603 6.5e-15 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1614
AarI CACCTGC 1 cut(s) 739
AasI GACNNNNNNGTC 1 cut(s) 1879
Acc36I ACCTGC 1 cut(s) 739
AccI GTMKAC 1 cut(s) 809
AccII CGCG 2 cut(s) 479, 1161
AciI CCGC 2 cut(s) 1310, 1653
AclI AACGTT 1 cut(s) 673
AclWI GGATC 2 cut(s) 1180, 1471
AcoI YGGCCR 2 cut(s) 794, 1166
AcsI RAATTY 3 cut(s) 251, 898, 1759
AcuI CTGAAG 3 cut(s) 428, 803, 1481
AfaI GTAC 8 cut(s) 413, 815, 844, 1274, 1349, 1517, 1548, 1775
AfiI CCNNNNNNNGG 2 cut(s) 890, 1175
AflIII ACRYGT 3 cut(s) 910, 1565, 1639
AgsI TTSAA 7 cut(s) 273, 386, 439, 1627, 1691, 1711, 1758
AhlI ACTAGT 2 cut(s) 614, 773
AjnI CCWGG 3 cut(s) 757, 889, 1168
AluBI AGCT 9 cut(s) 442, 448, 536, 689, 947, 1006, 1504, 1623, 1738
AluI AGCT 9 cut(s) 442, 448, 536, 689, 947, 1006, 1504, 1623, 1738
Alw21I GWGCWC 4 cut(s) 132, 368, 730, 1365
Alw26I GTCTC 2 cut(s) 1008, 1450
Alw44I GTGCAC 1 cut(s) 726
AlwI GGATC 2 cut(s) 1180, 1471
Ama87I CYCGRG 1 cut(s) 407
AoxI GGCC 3 cut(s) 794, 1166, 1842
ApaLI GTGCAC 1 cut(s) 726
ApeKI GCWGC 2 cut(s) 1003, 1620
ApoI RAATTY 3 cut(s) 251, 898, 1759
ArsI GACNNNNNNTTYG 4 cut(s) 458, 490, 1712, 1744
AspLEI GCGC 1 cut(s) 481
AspS9I GGNCC 2 cut(s) 659, 1087
AsuHPI GGTGA 2 cut(s) 253, 938
AvaI CYCGRG 1 cut(s) 407
AvaII GGWCC 2 cut(s) 659, 1087
AxyI CCTNAGG 1 cut(s) 330
BaeGI GKGCMC 1 cut(s) 730
BalI TGGCCA 2 cut(s) 796, 1168
BauI CACGAG 1 cut(s) 567
Bbv12I GWGCWC 4 cut(s) 132, 368, 730, 1365
BbvI GCAGC 2 cut(s) 1015, 1632
BccI CCATC 2 cut(s) 741, 944
BcgI CGANNNNNNTGC 2 cut(s) 992, 1026
BciT130I CCWGG 3 cut(s) 759, 891, 1170
BclI TGATCA 2 cut(s) 450, 1750
BcoDI GTCTC 2 cut(s) 1008, 1450
BcuI ACTAGT 2 cut(s) 614, 773
BfaI CTAG 4 cut(s) 212, 615, 774, 1877
BfmI CTRYAG 1 cut(s) 34
BfuAI ACCTGC 1 cut(s) 739
BisI GCNGC 2 cut(s) 1004, 1621
BlpI GCTNAGC 1 cut(s) 1369
BlsI GCNGC 2 cut(s) 1005, 1622
Bme1390I CCNGG 3 cut(s) 759, 891, 1170
Bme18I GGWCC 2 cut(s) 659, 1087
BmeT110I CYCGRG 1 cut(s) 407
BmgT120I GGNCC 2 cut(s) 659, 1087
BmiI GGNNCC 3 cut(s) 660, 955, 1088
BmrFI CCNGG 3 cut(s) 759, 891, 1170
BmsI GCATC 3 cut(s) 887, 1127, 1382
BplI GAGNNNNNCTC 4 cut(s) 970, 1002, 972, 1004
BpmI CTGGAG 1 cut(s) 385
Bpu1102I GCTNAGC 1 cut(s) 1369
BsaAI YACGTR 1 cut(s) 813
BsaI GGTCTC 1 cut(s) 1008
BsaJI CCNNGG 3 cut(s) 757, 1397, 1723
Bsc4I CCNNNNNNNGG 2 cut(s) 890, 1175
Bse1I ACTGG 4 cut(s) 402, 427, 557, 1169
Bse21I CCTNAGG 1 cut(s) 330
Bse3DI GCAATG 1 cut(s) 1527
BseBI CCWGG 3 cut(s) 759, 891, 1170
BseDI CCNNGG 3 cut(s) 757, 1397, 1723
BseGI GGATG 5 cut(s) 57, 102, 752, 946, 1682
BseLI CCNNNNNNNGG 2 cut(s) 890, 1175
BseMI GCAATG 1 cut(s) 1527
BseMII CTCAG 2 cut(s) 267, 321
BseNI ACTGG 4 cut(s) 402, 427, 557, 1169
BseRI GAGGAG 2 cut(s) 681, 1221
BseSI GKGCMC 1 cut(s) 730
BseXI GCAGC 2 cut(s) 1015, 1632
BseYI CCCAGC 2 cut(s) 685, 820
BsgI GTGCAG 4 cut(s) 354, 1083, 1677, 1831
Bsh1236I CGCG 2 cut(s) 479, 1161
BshFI GGCC 3 cut(s) 796, 1168, 1844
BsiHKAI GWGCWC 4 cut(s) 132, 368, 730, 1365
BsiHKCI CYCGRG 1 cut(s) 407
BslFI GGGAC 6 cut(s) 390, 837, 1100, 1554, 1802, 1847
BslI CCNNNNNNNGG 2 cut(s) 890, 1175
BsmAI GTCTC 2 cut(s) 1008, 1450
BsmFI GGGAC 6 cut(s) 390, 837, 1100, 1554, 1802, 1847
BsnI GGCC 3 cut(s) 796, 1168, 1844
Bso31I GGTCTC 1 cut(s) 1008
BsoBI CYCGRG 1 cut(s) 407
Bsp1286I GDGCHC 4 cut(s) 132, 368, 730, 1365
Bsp1407I TGTACA 1 cut(s) 1546
Bsp143I GATC 6 cut(s) 450, 461, 706, 1172, 1463, 1750
Bsp1720I GCTNAGC 1 cut(s) 1369
Bsp19I CCATGG 2 cut(s) 1397, 1723
BspACI CCGC 2 cut(s) 1310, 1653
BspANI GGCC 3 cut(s) 796, 1168, 1844
BspCNI CTCAG 2 cut(s) 268, 322
BspFNI CGCG 2 cut(s) 479, 1161
BspHI TCATGA 3 cut(s) 154, 175, 1883
BspLI GGNNCC 3 cut(s) 660, 955, 1088
BspMI ACCTGC 1 cut(s) 739
BspPI GGATC 2 cut(s) 1180, 1471
BspQI GCTCTTC 2 cut(s) 177, 1775
BspTNI GGTCTC 1 cut(s) 1008
BsrDI GCAATG 1 cut(s) 1527
BsrGI TGTACA 1 cut(s) 1546
BsrI ACTGG 4 cut(s) 402, 427, 557, 1169
BssECI CCNNGG 3 cut(s) 757, 1397, 1723
BssMI GATC 6 cut(s) 450, 461, 706, 1172, 1463, 1750
BssNAI GTATAC 1 cut(s) 810
BssSI CACGAG 1 cut(s) 567
BssT1I CCWWGG 2 cut(s) 1397, 1723
Bst1107I GTATAC 1 cut(s) 810
Bst2BI CACGAG 1 cut(s) 567
Bst2UI CCWGG 3 cut(s) 759, 891, 1170
Bst4CI ACNGT 6 cut(s) 44, 208, 604, 742, 1126, 1852
Bst6I CTCTTC 3 cut(s) 177, 1593, 1775
BstAPI GCANNNNNTGC 1 cut(s) 167
BstAUI TGTACA 1 cut(s) 1546
BstBAI YACGTR 1 cut(s) 813
BstC8I GCNNGC 2 cut(s) 1651, 1846
BstDEI CTNAG 5 cut(s) 276, 330, 999, 1263, 1369
BstDSI CCRYGG 2 cut(s) 1397, 1723
BstEII GGTNACC 1 cut(s) 1070
BstF5I GGATG 5 cut(s) 57, 102, 752, 946, 1682
BstFNI CGCG 2 cut(s) 479, 1161
BstHHI GCGC 1 cut(s) 481
BstKTI GATC 6 cut(s) 453, 464, 709, 1175, 1466, 1753
BstMAI GTCTC 2 cut(s) 1008, 1450
BstMBI GATC 6 cut(s) 450, 461, 706, 1172, 1463, 1750
BstMWI GCNNNNNNNGC 3 cut(s) 167, 1399, 1655
BstNI CCWGG 3 cut(s) 759, 891, 1170
BstNSI RCATGY 4 cut(s) 721, 1294, 1569, 1643
BstPI GGTNACC 1 cut(s) 1070
BstSCI CCNGG 3 cut(s) 757, 889, 1168
BstSFI CTRYAG 1 cut(s) 34
BstSLI GKGCMC 1 cut(s) 730
BstSNI TACGTA 1 cut(s) 813
BstUI CGCG 2 cut(s) 479, 1161
BstV1I GCAGC 2 cut(s) 1015, 1632
BstZ17I GTATAC 1 cut(s) 810
Bsu36I CCTNAGG 1 cut(s) 330
BsuRI GGCC 3 cut(s) 796, 1168, 1844
BtgI CCRYGG 2 cut(s) 1397, 1723
BtsCI GGATG 5 cut(s) 57, 102, 752, 946, 1682
BtsI GCAGTG 1 cut(s) 1363
BtsIMutI CAGTG 5 cut(s) 231, 420, 747, 1278, 1363
BveI ACCTGC 1 cut(s) 739
Cac8I GCNNGC 2 cut(s) 1651, 1846
CciI TCATGA 3 cut(s) 154, 175, 1883
CfoI GCGC 1 cut(s) 481
Cfr13I GGNCC 2 cut(s) 659, 1087
CseI GACGC 1 cut(s) 1869
Csp6I GTAC 8 cut(s) 412, 814, 843, 1273, 1348, 1516, 1547, 1774
CspCI CAANNNNNGTGG 6 cut(s) 495, 530, 723, 758, 1623, 1658
CviQI GTAC 8 cut(s) 412, 814, 843, 1273, 1348, 1516, 1547, 1774
DdeI CTNAG 5 cut(s) 276, 330, 999, 1263, 1369
DpnI GATC 6 cut(s) 452, 463, 708, 1174, 1465, 1752
DpnII GATC 6 cut(s) 450, 461, 706, 1172, 1463, 1750
DraI TTTAAA 2 cut(s) 27, 874
DrdI GACNNNNNNGTC 1 cut(s) 1879
DseDI GACNNNNNNGTC 1 cut(s) 1879
EaeI YGGCCR 2 cut(s) 794, 1166
Eam1104I CTCTTC 3 cut(s) 177, 1593, 1775
EarI CTCTTC 3 cut(s) 177, 1593, 1775
Eco105I TACGTA 1 cut(s) 813
Eco130I CCWWGG 2 cut(s) 1397, 1723
Eco31I GGTCTC 1 cut(s) 1008
Eco47I GGWCC 2 cut(s) 659, 1087
Eco57I CTGAAG 3 cut(s) 428, 803, 1481
Eco81I CCTNAGG 1 cut(s) 330
Eco88I CYCGRG 1 cut(s) 407
Eco91I GGTNACC 1 cut(s) 1070
EcoO65I GGTNACC 1 cut(s) 1070
EcoRII CCWGG 3 cut(s) 757, 889, 1168
EcoT14I CCWWGG 2 cut(s) 1397, 1723
ErhI CCWWGG 2 cut(s) 1397, 1723
FalI AAGNNNNNCTT 2 cut(s) 1607, 1639
FaqI GGGAC 6 cut(s) 390, 837, 1100, 1554, 1802, 1847
FbaI TGATCA 2 cut(s) 450, 1750
FblI GTMKAC 1 cut(s) 809
Fnu4HI GCNGC 2 cut(s) 1004, 1621
FokI GGATG 5 cut(s) 64, 89, 759, 953, 1669
Fsp4HI GCNGC 2 cut(s) 1004, 1621
FspBI CTAG 4 cut(s) 212, 615, 774, 1877
GlaI GCGC 1 cut(s) 480
GluI GCNGC 2 cut(s) 1004, 1621
GsaI CCCAGC 2 cut(s) 689, 824
GsuI CTGGAG 1 cut(s) 385
HaeIII GGCC 3 cut(s) 796, 1168, 1844
HgaI GACGC 1 cut(s) 1869
HhaI GCGC 1 cut(s) 481
Hin6I GCGC 1 cut(s) 479
HinP1I GCGC 1 cut(s) 479
HincII GTYRAC 1 cut(s) 1525
HindII GTYRAC 1 cut(s) 1525
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 4 cut(s) 502, 762, 856, 1199
HphI GGTGA 2 cut(s) 253, 938
Hpy166II GTNNAC 4 cut(s) 497, 728, 810, 1525
Hpy188I TCNGA 7 cut(s) 20, 262, 466, 783, 1430, 1500, 1814
Hpy8I GTNNAC 4 cut(s) 497, 728, 810, 1525
HpyAV CCTTC 6 cut(s) 19, 279, 778, 1454, 1505, 1697
HpyCH4III ACNGT 6 cut(s) 44, 208, 604, 742, 1126, 1852
HpyCH4IV ACGT 3 cut(s) 673, 812, 910
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 1399, 1655
HpyF3I CTNAG 5 cut(s) 276, 330, 999, 1263, 1369
HpySE526I ACGT 3 cut(s) 673, 812, 910
HspAI GCGC 1 cut(s) 479
Ksp22I TGATCA 2 cut(s) 450, 1750
Kzo9I GATC 6 cut(s) 450, 461, 706, 1172, 1463, 1750
LguI GCTCTTC 2 cut(s) 177, 1775
LmnI GCTCC 1 cut(s) 1661
Lsp1109I GCAGC 2 cut(s) 1015, 1632
LweI GCATC 3 cut(s) 887, 1127, 1382
MaeI CTAG 4 cut(s) 212, 615, 774, 1877
MaeII ACGT 3 cut(s) 673, 812, 910
MaeIII GTNAC 6 cut(s) 241, 604, 691, 752, 926, 1070
MalI GATC 6 cut(s) 452, 463, 708, 1174, 1465, 1752
MboI GATC 6 cut(s) 450, 461, 706, 1172, 1463, 1750
MboII GAAGA 8 cut(s) 4, 7, 164, 312, 1428, 1469, 1610, 1792
MhlI GDGCHC 4 cut(s) 132, 368, 730, 1365
MlsI TGGCCA 2 cut(s) 796, 1168
MluNI TGGCCA 2 cut(s) 796, 1168
MlyI GAGTC 1 cut(s) 865
Mox20I TGGCCA 2 cut(s) 796, 1168
MscI TGGCCA 2 cut(s) 796, 1168
MseI TTAA 6 cut(s) 26, 80, 218, 378, 873, 1470
MslI CAYNNNNRTG 3 cut(s) 361, 850, 1554
Msp20I TGGCCA 2 cut(s) 796, 1168
MspA1I CMGCKG 2 cut(s) 448, 689
MspR9I CCNGG 3 cut(s) 759, 891, 1170
MvaI CCWGG 3 cut(s) 759, 891, 1170
MvnI CGCG 2 cut(s) 479, 1161
MwoI GCNNNNNNNGC 3 cut(s) 167, 1399, 1655
NcoI CCATGG 2 cut(s) 1397, 1723
NdeII GATC 6 cut(s) 450, 461, 706, 1172, 1463, 1750
NlaIV GGNNCC 3 cut(s) 660, 955, 1088
NmuCI GTSAC 4 cut(s) 241, 691, 752, 926
NspI RCATGY 4 cut(s) 721, 1294, 1569, 1643
PagI TCATGA 3 cut(s) 154, 175, 1883
PaqCI CACCTGC 1 cut(s) 739
PciI ACATGT 2 cut(s) 1565, 1639
PciSI GCTCTTC 2 cut(s) 177, 1775
PcsI WCGNNNNNNNCGW 1 cut(s) 1877
PfeI GAWTC 3 cut(s) 502, 762, 1199
PkrI GCNGC 2 cut(s) 1005, 1622
PleI GAGTC 1 cut(s) 864
PpsI GAGTC 1 cut(s) 864
Ppu21I YACGTR 1 cut(s) 813
PscI ACATGT 2 cut(s) 1565, 1639
PsiI TTATAA 1 cut(s) 1614
Psp1406I AACGTT 1 cut(s) 673
Psp6I CCWGG 3 cut(s) 757, 889, 1168
PspEI GGTNACC 1 cut(s) 1070
PspFI CCCAGC 2 cut(s) 685, 820
PspGI CCWGG 3 cut(s) 757, 889, 1168
PspN4I GGNNCC 3 cut(s) 660, 955, 1088
PspPI GGNCC 2 cut(s) 659, 1087
PvuII CAGCTG 2 cut(s) 448, 689
RsaI GTAC 8 cut(s) 413, 815, 844, 1274, 1349, 1517, 1548, 1775
RsaNI GTAC 8 cut(s) 412, 814, 843, 1273, 1348, 1516, 1547, 1774
RseI CAYNNNNRTG 3 cut(s) 361, 850, 1554
SapI GCTCTTC 2 cut(s) 177, 1775
SaqAI TTAA 6 cut(s) 26, 80, 218, 378, 873, 1470
SatI GCNGC 2 cut(s) 1004, 1621
Sau3AI GATC 6 cut(s) 450, 461, 706, 1172, 1463, 1750
Sau96I GGNCC 2 cut(s) 659, 1087
SchI GAGTC 1 cut(s) 865
ScrFI CCNGG 3 cut(s) 759, 891, 1170
SduI GDGCHC 4 cut(s) 132, 368, 730, 1365
SfaNI GCATC 3 cut(s) 887, 1127, 1382
SfcI CTRYAG 1 cut(s) 34
SinI GGWCC 2 cut(s) 659, 1087
SmiMI CAYNNNNRTG 3 cut(s) 361, 850, 1554
SnaBI TACGTA 1 cut(s) 813
SpeI ACTAGT 2 cut(s) 614, 773
SsiI CCGC 2 cut(s) 1310, 1653
SspI AATATT 1 cut(s) 1585
SspMI CTAG 4 cut(s) 212, 615, 774, 1877
StyD4I CCNGG 3 cut(s) 757, 889, 1168
StyI CCWWGG 2 cut(s) 1397, 1723
TaaI ACNGT 6 cut(s) 44, 208, 604, 742, 1126, 1852
TaiI ACGT 3 cut(s) 676, 815, 913
TaqI TCGA 2 cut(s) 1012, 1770
TatI WGTACW 4 cut(s) 411, 1515, 1546, 1773
TfiI GAWTC 3 cut(s) 502, 762, 1199
Tru1I TTAA 6 cut(s) 26, 80, 218, 378, 873, 1470
Tru9I TTAA 6 cut(s) 26, 80, 218, 378, 873, 1470
TscAI CASTG 5 cut(s) 238, 427, 747, 1285, 1370
TseFI GTSAC 4 cut(s) 241, 691, 752, 926
TseI GCWGC 2 cut(s) 1003, 1620
Tsp45I GTSAC 4 cut(s) 241, 691, 752, 926
TspGWI ACGGA 1 cut(s) 1806
TspRI CASTG 5 cut(s) 238, 427, 747, 1285, 1370
VneI GTGCAC 1 cut(s) 726
VpaK11BI GGWCC 2 cut(s) 659, 1087
XapI RAATTY 3 cut(s) 251, 898, 1759
XceI RCATGY 4 cut(s) 721, 1294, 1569, 1643
XmiI GTMKAC 1 cut(s) 809
XspI CTAG 4 cut(s) 212, 615, 774, 1877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.