Rroxscaffold_5G00379390

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002060
Physical Location & Seq
Reverse (-)
59788636 .. 59793101
4466 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00379390.1

Sequence Viewer

Length: 1866 bp
ATGCTTCAGTTACTTCCCCACAGGTTGAGAACGATGAAAGCTGTTACCTTGTTTGCAATCACATTGGTGCTTGCAACAGTTCAGCATGTGAGGTCTGATGGAGATCAGCCCTTGTCGAAGATTGCGGTTCACCGGGCGTTATCTGCTCTTCATGACAAAGCTTATGTTAAAGCCTCCCCTGAGCTTCTTGGACTGAAGGGGGAAACTTCAGTGTGGGTAAATCTGCAGTTCAGTTCTCCTCACCCTTCAAGTGATGATTGGATTGGAGTATTTTCTCCTGCAAATTTCAGTGCTTCAACCTGCGCTGCAGAAAATTCTGTGATGACTGCTCCATTTTTATGTTCTGCACCAATTAAGTACCAGTATGCAAATTACTCTAGTCCGAAGTACCAAGAGACAGGGAGAGGCTCGTTGAAGTTACAGTTGATTAACCAGAGATCAGACTTCTCTTTTGCACTATTCTCCGGTGGATTGTCAAACCCAAAGCTGGTGTCAATCTCTGGTAGAGTATCATTCGCCAATCCAAAAGCTCCAGTTTATCCACGCTTAGCACAAGGGAAAATGTGGAATGAGATGACTGTAACATGGACAAGTGGATATGCTCTAGGGGAAGCAACACCTTTTGTTGAATGGGGTGAGAAAGGAGGAGACCTTGTGAGATCCCCAGCTGGGACTTTGACCTTCGATCGCAACAGCATGTGTGGTGCACCAGCAAGAACAGTGGGATGGCGAGATCCTGGATATATACACACTGCTTTTCTGAAGGAATTGTGGCCCAATACAGTGTATATCTACAAACTAGGACACAAATTGTCAAATGGTTCATATGTTTTCAGTCGACAATACCAGTTCAGAGCCTCTCCTTATCCTGGACAAAATTCTCCTCAAAGTATTGTTATATTTGGTGACATGGGAAAGGATGAAGAGGATGGTTCCAATGAATACAACAATTTCCAGAGAGGCTCTCTCAACACTACTAAGCAGCTTATTCGAGACCTGAAAAACATTGATATTGTGTTCCATATTGGAGATATATGTTATGCAAATGGTTACCTTTCACAATGGGACCAATTTACTGCACAGGTTGAGCCAATAGCATCTTCTGTTCCTTACATGGTTGCAAGTGGAAATCACGAGCGTGACTGGCCTAATTCAGGTTCCTTTTTTGGAAGGACAGATTCAGGTGGGGAATGTGGTGTCTTAGCCCAGAACATGTTTTATGTCCCTGCTGAGAACAGGGAAAAATTCTGGTATTCCACTGACTATGGCATGTTCCGGTTCTGCATAGGCGACACAGAACATGATTGGAGAGAAGGAACAGAGCAGTACAAGTTCATTGAGAACTGCTTTGCAACAGTTGATAGACAAAAGCAACCATGGCTGATATTTCTTGCACATCGGGTATTGGGTTACTCTTCGGCGAGCTTTTATGTAGCTGAAGGATCGTTTGAGGAACCAATGGGAAGGGAGAGCCTTCAGAAACTATGGCAGAAATACAAGGTTGACATTGCCGTATATGGCCATGTGCACAATTATGAAAGGACATGTCCCATTTACGAGAATATCTGCACTAGCAAGGAGAAGCAAAATTACGTAGGTAATCTGAATGGGACAATCCATGTGGTTGCTGGCGGTGGAGGAGCAAGTCTTGTAACCTTTGCTCCTATTAATACGACATGGAGTATCTTTAAGGACTATGACTATGGATTTGTCAAAATGACTGCATTTGATCACTCAAACCTGTTGTTCGAGTACAAGAAGAGCAGTGATGGAAAGGTTTATGACTCTTTCAGAATATCCAAGAATTACAGAGACATCTTGGCCTGCAGTGTTGATAGTTGCCCAAGTACAGCAATGGCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

621

Amino Acids

69.61

Weight (kDa)

7.21

Isoelectric Point (pI)

37.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_PAP PF17808 56 - 174 7.6e-44 Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N PF16656 181 - 285 8.3e-14 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 298 - 512 2.8e-17 Calcineurin-like phosphoesterase
Metallophos_C PF14008 537 - 596 4.7e-15 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 308
AccI GTMKAC 1 cut(s) 838
AciI CCGC 2 cut(s) 125, 1632
AclWI GGATC 3 cut(s) 654, 728, 1450
AcoI YGGCCR 1 cut(s) 1519
AcsI RAATTY 4 cut(s) 283, 313, 877, 1244
AcuI CTGAAG 5 cut(s) 192, 215, 782, 1458, 1460
AfaI GTAC 5 cut(s) 359, 389, 1328, 1754, 1849
AfiI CCNNNNNNNGG 4 cut(s) 487, 669, 869, 1154
AflIII ACRYGT 2 cut(s) 1212, 1544
AgsI TTSAA 4 cut(s) 249, 297, 415, 629
AjnI CCWGG 2 cut(s) 736, 868
AjuI GAANNNNNNNTTGG 2 cut(s) 1084, 1116
AleI CACNNNNGTG 2 cut(s) 65, 1137
AluBI AGCT 9 cut(s) 41, 161, 184, 487, 530, 668, 985, 1425, 1436
AluI AGCT 9 cut(s) 41, 161, 184, 487, 530, 668, 985, 1425, 1436
Alw21I GWGCWC 2 cut(s) 709, 1530
Alw26I GTCTC 4 cut(s) 389, 642, 987, 1806
Alw44I GTGCAC 2 cut(s) 705, 1526
AlwI GGATC 3 cut(s) 654, 728, 1450
AoxI GGCC 4 cut(s) 773, 1145, 1519, 1821
ApaLI GTGCAC 2 cut(s) 705, 1526
ApeKI GCWGC 2 cut(s) 305, 982
ApoI RAATTY 4 cut(s) 283, 313, 877, 1244
ArsI GACNNNNNNTTYG 6 cut(s) 434, 466, 476, 508, 1691, 1723
AseI ATTAAT 1 cut(s) 1668
AspLEI GCGC 1 cut(s) 305
AspS9I GGNCC 2 cut(s) 774, 1066
AsuC2I CCSGG 1 cut(s) 134
AsuHPI GGTGA 4 cut(s) 122, 233, 647, 917
AvaII GGWCC 1 cut(s) 1066
BaeGI GKGCMC 2 cut(s) 709, 1530
BalI TGGCCA 1 cut(s) 1521
BarI GAAGNNNNNNTAC 2 cut(s) 1574, 1606
BauI CACGAG 1 cut(s) 1133
Bbv12I GWGCWC 2 cut(s) 709, 1530
BbvI GCAGC 2 cut(s) 292, 994
BccI CCATC 4 cut(s) 92, 720, 923, 1763
BceAI ACGGC 1 cut(s) 1496
BcgI CGANNNNNNTGC 2 cut(s) 971, 1005
BciT130I CCWGG 2 cut(s) 738, 870
BclI TGATCA 1 cut(s) 1729
BcnI CCSGG 1 cut(s) 134
BcoDI GTCTC 4 cut(s) 389, 642, 987, 1806
BfaI CTAG 4 cut(s) 378, 605, 800, 1572
BfmI CTRYAG 3 cut(s) 224, 306, 1825
BfuAI ACCTGC 1 cut(s) 308
BisI GCNGC 2 cut(s) 306, 983
BlpI GCTNAGC 1 cut(s) 547
BlsI GCNGC 2 cut(s) 307, 984
Bme1390I CCNGG 3 cut(s) 134, 738, 870
Bme18I GGWCC 1 cut(s) 1066
BmgT120I GGNCC 2 cut(s) 774, 1066
BmiI GGNNCC 4 cut(s) 934, 1067, 1159, 1455
BmrFI CCNGG 3 cut(s) 134, 738, 870
BmsI GCATC 1 cut(s) 1106
BplI GAGNNNNNCTC 4 cut(s) 949, 981, 951, 983
BpmI CTGGAG 1 cut(s) 516
Bpu10I CCTNAGC 1 cut(s) 180
Bpu1102I GCTNAGC 1 cut(s) 547
BpuMI CCSGG 1 cut(s) 134
BsaAI YACGTR 1 cut(s) 1594
BsaBI GATNNNNATC 1 cut(s) 102
BsaI GGTCTC 2 cut(s) 642, 987
BsaJI CCNNGG 1 cut(s) 1376
BsaWI WCCGGW 2 cut(s) 464, 1275
BsaXI ACNNNNNCTCC 4 cut(s) 639, 669, 1645, 1675
Bsc4I CCNNNNNNNGG 4 cut(s) 487, 669, 869, 1154
Bse1I ACTGG 4 cut(s) 361, 533, 847, 1148
Bse3DI GCAATG 2 cut(s) 1506, 1860
Bse8I GATNNNNATC 1 cut(s) 102
BseBI CCWGG 2 cut(s) 738, 870
BseDI CCNNGG 1 cut(s) 1376
BseGI GGATG 3 cut(s) 731, 925, 934
BseJI GATNNNNATC 1 cut(s) 102
BseLI CCNNNNNNNGG 4 cut(s) 487, 669, 869, 1154
BseMI GCAATG 2 cut(s) 1506, 1860
BseMII CTCAG 2 cut(s) 171, 1221
BseNI ACTGG 4 cut(s) 361, 533, 847, 1148
BseRI GAGGAG 4 cut(s) 228, 660, 873, 1653
BseSI GKGCMC 2 cut(s) 709, 1530
BseXI GCAGC 2 cut(s) 292, 994
BseYI CCCAGC 2 cut(s) 664, 668
BsgI GTGCAG 3 cut(s) 330, 1062, 1552
Bsh1285I CGRYCG 1 cut(s) 688
BshFI GGCC 4 cut(s) 775, 1147, 1521, 1823
BsiEI CGRYCG 1 cut(s) 688
BsiHKAI GWGCWC 2 cut(s) 709, 1530
BsiSI CCGG 3 cut(s) 133, 465, 1276
BslFI GGGAC 5 cut(s) 685, 1079, 1208, 1533, 1624
BslI CCNNNNNNNGG 4 cut(s) 487, 669, 869, 1154
BsmAI GTCTC 4 cut(s) 389, 642, 987, 1806
BsmFI GGGAC 5 cut(s) 685, 1079, 1208, 1533, 1624
BsnI GGCC 4 cut(s) 775, 1147, 1521, 1823
Bso31I GGTCTC 2 cut(s) 642, 987
Bsp1286I GDGCHC 2 cut(s) 709, 1530
Bsp143I GATC 7 cut(s) 103, 437, 659, 685, 733, 1442, 1729
Bsp1720I GCTNAGC 1 cut(s) 547
Bsp19I CCATGG 1 cut(s) 1376
BspACI CCGC 2 cut(s) 125, 1632
BspANI GGCC 4 cut(s) 775, 1147, 1521, 1823
BspCNI CTCAG 2 cut(s) 172, 1222
BspHI TCATGA 1 cut(s) 151
BspLI GGNNCC 4 cut(s) 934, 1067, 1159, 1455
BspMAI CTGCAG 3 cut(s) 228, 310, 1829
BspMI ACCTGC 1 cut(s) 308
BspPI GGATC 3 cut(s) 654, 728, 1450
BspQI GCTCTTC 2 cut(s) 153, 1754
BspTNI GGTCTC 2 cut(s) 642, 987
BsrDI GCAATG 2 cut(s) 1506, 1860
BsrI ACTGG 4 cut(s) 361, 533, 847, 1148
BssECI CCNNGG 1 cut(s) 1376
BssMI GATC 7 cut(s) 103, 437, 659, 685, 733, 1442, 1729
BssSI CACGAG 1 cut(s) 1133
BssT1I CCWWGG 1 cut(s) 1376
Bst2BI CACGAG 1 cut(s) 1133
Bst2UI CCWGG 2 cut(s) 738, 870
Bst4CI ACNGT 6 cut(s) 79, 423, 580, 721, 784, 1357
Bst6I CTCTTC 4 cut(s) 153, 918, 1420, 1754
BstBAI YACGTR 1 cut(s) 1594
BstC8I GCNNGC 4 cut(s) 72, 1423, 1630, 1825
BstDEI CTNAG 5 cut(s) 180, 547, 978, 1201, 1230
BstDSI CCRYGG 1 cut(s) 1376
BstEII GGTNACC 1 cut(s) 1049
BstENI CCTNNNNNAGG 1 cut(s) 1152
BstF5I GGATG 3 cut(s) 731, 925, 934
BstHHI GCGC 1 cut(s) 305
BstKTI GATC 7 cut(s) 106, 440, 662, 688, 736, 1445, 1732
BstMAI GTCTC 4 cut(s) 389, 642, 987, 1806
BstMBI GATC 7 cut(s) 103, 437, 659, 685, 733, 1442, 1729
BstMCI CGRYCG 1 cut(s) 688
BstMWI GCNNNNNNNGC 3 cut(s) 143, 1144, 1378
BstNI CCWGG 2 cut(s) 738, 870
BstNSI RCATGY 5 cut(s) 89, 700, 1216, 1273, 1548
BstPI GGTNACC 1 cut(s) 1049
BstSCI CCNGG 3 cut(s) 132, 736, 868
BstSFI CTRYAG 3 cut(s) 224, 306, 1825
BstSLI GKGCMC 2 cut(s) 709, 1530
BstSNI TACGTA 1 cut(s) 1594
BstV1I GCAGC 2 cut(s) 292, 994
BstX2I RGATCY 2 cut(s) 659, 733
BstYI RGATCY 2 cut(s) 659, 733
BsuRI GGCC 4 cut(s) 775, 1147, 1521, 1823
BtgI CCRYGG 1 cut(s) 1376
BtsCI GGATG 3 cut(s) 731, 925, 934
BtsI GCAGTG 3 cut(s) 750, 1771, 1834
BtsIMutI CAGTG 8 cut(s) 216, 295, 726, 750, 789, 1257, 1771, 1834
BveI ACCTGC 1 cut(s) 308
Cac8I GCNNGC 4 cut(s) 72, 1423, 1630, 1825
CciI TCATGA 1 cut(s) 151
CfoI GCGC 1 cut(s) 305
Cfr13I GGNCC 2 cut(s) 774, 1066
Csp6I GTAC 5 cut(s) 358, 388, 1327, 1753, 1848
CspCI CAANNNNNGTGG 4 cut(s) 702, 737, 1602, 1637
CviQI GTAC 5 cut(s) 358, 388, 1327, 1753, 1848
DdeI CTNAG 5 cut(s) 180, 547, 978, 1201, 1230
DpnI GATC 7 cut(s) 105, 439, 661, 687, 735, 1444, 1731
DpnII GATC 7 cut(s) 103, 437, 659, 685, 733, 1442, 1729
EaeI YGGCCR 1 cut(s) 1519
Eam1104I CTCTTC 4 cut(s) 153, 918, 1420, 1754
EarI CTCTTC 4 cut(s) 153, 918, 1420, 1754
Eco105I TACGTA 1 cut(s) 1594
Eco130I CCWWGG 1 cut(s) 1376
Eco31I GGTCTC 2 cut(s) 642, 987
Eco47I GGWCC 1 cut(s) 1066
Eco57I CTGAAG 5 cut(s) 192, 215, 782, 1458, 1460
Eco91I GGTNACC 1 cut(s) 1049
EcoNI CCTNNNNNAGG 1 cut(s) 1152
EcoO65I GGTNACC 1 cut(s) 1049
EcoRII CCWGG 2 cut(s) 736, 868
EcoT14I CCWWGG 1 cut(s) 1376
ErhI CCWWGG 1 cut(s) 1376
FaqI GGGAC 5 cut(s) 685, 1079, 1208, 1533, 1624
FauNDI CATATG 1 cut(s) 826
FbaI TGATCA 1 cut(s) 1729
FblI GTMKAC 1 cut(s) 838
Fnu4HI GCNGC 2 cut(s) 306, 983
FokI GGATG 3 cut(s) 738, 932, 941
Fsp4HI GCNGC 2 cut(s) 306, 983
FspBI CTAG 4 cut(s) 378, 605, 800, 1572
GlaI GCGC 1 cut(s) 304
GluI GCNGC 2 cut(s) 306, 983
GsaI CCCAGC 2 cut(s) 668, 672
GsuI CTGGAG 1 cut(s) 516
HaeIII GGCC 4 cut(s) 775, 1147, 1521, 1823
HapII CCGG 3 cut(s) 133, 465, 1276
HhaI GCGC 1 cut(s) 305
Hin6I GCGC 1 cut(s) 303
HinP1I GCGC 1 cut(s) 303
HincII GTYRAC 2 cut(s) 839, 1504
HindII GTYRAC 2 cut(s) 839, 1504
HindIII AAGCTT 1 cut(s) 159
HinfI GANTC 2 cut(s) 1178, 1784
HpaII CCGG 3 cut(s) 133, 465, 1276
HphI GGTGA 4 cut(s) 122, 233, 647, 917
Hpy166II GTNNAC 5 cut(s) 130, 707, 839, 1504, 1528
Hpy188I TCNGA 8 cut(s) 97, 384, 442, 762, 854, 1479, 1605, 1793
Hpy188III TCNNGA 5 cut(s) 152, 955, 992, 1133, 1863
Hpy8I GTNNAC 5 cut(s) 130, 707, 839, 1504, 1528
HpyAV CCTTC 9 cut(s) 190, 255, 691, 757, 1164, 1307, 1433, 1458, 1484
HpyCH4III ACNGT 6 cut(s) 79, 423, 580, 721, 784, 1357
HpyCH4IV ACGT 1 cut(s) 1593
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 1144, 1378
HpyF3I CTNAG 5 cut(s) 180, 547, 978, 1201, 1230
HpySE526I ACGT 1 cut(s) 1593
HspAI GCGC 1 cut(s) 303
Ksp22I TGATCA 1 cut(s) 1729
Kzo9I GATC 7 cut(s) 103, 437, 659, 685, 733, 1442, 1729
LguI GCTCTTC 2 cut(s) 153, 1754
LmnI GCTCC 4 cut(s) 334, 535, 1640, 1666
Lsp1109I GCAGC 2 cut(s) 292, 994
LweI GCATC 1 cut(s) 1106
MaeI CTAG 4 cut(s) 378, 605, 800, 1572
MaeII ACGT 1 cut(s) 1593
MaeIII GTNAC 9 cut(s) 9, 43, 417, 580, 905, 1049, 1139, 1409, 1651
MalI GATC 7 cut(s) 105, 439, 661, 687, 735, 1444, 1731
MboI GATC 7 cut(s) 103, 437, 659, 685, 733, 1442, 1729
MboII GAAGA 6 cut(s) 130, 140, 935, 1092, 1407, 1771
MflI RGATCY 2 cut(s) 659, 733
MhlI GDGCHC 2 cut(s) 709, 1530
MlsI TGGCCA 1 cut(s) 1521
MluNI TGGCCA 1 cut(s) 1521
MlyI GAGTC 1 cut(s) 1778
Mox20I TGGCCA 1 cut(s) 1521
MscI TGGCCA 1 cut(s) 1521
MseI TTAA 5 cut(s) 168, 354, 429, 1668, 1689
MslI CAYNNNNRTG 4 cut(s) 65, 337, 1137, 1533
Msp20I TGGCCA 1 cut(s) 1521
MspA1I CMGCKG 1 cut(s) 668
MspI CCGG 3 cut(s) 133, 465, 1276
MspR9I CCNGG 3 cut(s) 134, 738, 870
MvaI CCWGG 2 cut(s) 738, 870
MwoI GCNNNNNNNGC 3 cut(s) 143, 1144, 1378
NciI CCSGG 1 cut(s) 134
NcoI CCATGG 1 cut(s) 1376
NdeI CATATG 1 cut(s) 826
NdeII GATC 7 cut(s) 103, 437, 659, 685, 733, 1442, 1729
NlaIV GGNNCC 4 cut(s) 934, 1067, 1159, 1455
NmuCI GTSAC 2 cut(s) 905, 1139
NspI RCATGY 5 cut(s) 89, 700, 1216, 1273, 1548
OliI CACNNNNGTG 2 cut(s) 65, 1137
PagI TCATGA 1 cut(s) 151
PciI ACATGT 2 cut(s) 1212, 1544
PciSI GCTCTTC 2 cut(s) 153, 1754
PfeI GAWTC 1 cut(s) 1178
PfoI TCCNGGA 2 cut(s) 736, 868
PkrI GCNGC 2 cut(s) 307, 984
Ple19I CGATCG 1 cut(s) 688
PleI GAGTC 1 cut(s) 1778
PpsI GAGTC 1 cut(s) 1778
Ppu21I YACGTR 1 cut(s) 1594
PscI ACATGT 2 cut(s) 1212, 1544
PshBI ATTAAT 1 cut(s) 1668
Psp6I CCWGG 2 cut(s) 736, 868
PspEI GGTNACC 1 cut(s) 1049
PspFI CCCAGC 2 cut(s) 664, 668
PspGI CCWGG 2 cut(s) 736, 868
PspN4I GGNNCC 4 cut(s) 934, 1067, 1159, 1455
PspPI GGNCC 2 cut(s) 774, 1066
PstI CTGCAG 3 cut(s) 228, 310, 1829
PsuI RGATCY 2 cut(s) 659, 733
PvuI CGATCG 1 cut(s) 688
PvuII CAGCTG 1 cut(s) 668
RsaI GTAC 5 cut(s) 359, 389, 1328, 1754, 1849
RsaNI GTAC 5 cut(s) 358, 388, 1327, 1753, 1848
RseI CAYNNNNRTG 4 cut(s) 65, 337, 1137, 1533
SalI GTCGAC 1 cut(s) 837
SapI GCTCTTC 2 cut(s) 153, 1754
SaqAI TTAA 5 cut(s) 168, 354, 429, 1668, 1689
SatI GCNGC 2 cut(s) 306, 983
Sau3AI GATC 7 cut(s) 103, 437, 659, 685, 733, 1442, 1729
Sau96I GGNCC 2 cut(s) 774, 1066
SchI GAGTC 1 cut(s) 1778
ScrFI CCNGG 3 cut(s) 134, 738, 870
SduI GDGCHC 2 cut(s) 709, 1530
SfaNI GCATC 1 cut(s) 1106
SfcI CTRYAG 3 cut(s) 224, 306, 1825
SinI GGWCC 1 cut(s) 1066
SmiMI CAYNNNNRTG 4 cut(s) 65, 337, 1137, 1533
SnaBI TACGTA 1 cut(s) 1594
SsiI CCGC 2 cut(s) 125, 1632
SspMI CTAG 4 cut(s) 378, 605, 800, 1572
StyD4I CCNGG 3 cut(s) 132, 736, 868
StyI CCWWGG 1 cut(s) 1376
TaaI ACNGT 6 cut(s) 79, 423, 580, 721, 784, 1357
TaiI ACGT 1 cut(s) 1596
TaqI TCGA 5 cut(s) 116, 684, 838, 991, 1749
TatI WGTACW 3 cut(s) 1326, 1752, 1847
TfiI GAWTC 1 cut(s) 1178
Tru1I TTAA 5 cut(s) 168, 354, 429, 1668, 1689
Tru9I TTAA 5 cut(s) 168, 354, 429, 1668, 1689
TscAI CASTG 8 cut(s) 216, 295, 726, 757, 789, 1264, 1771, 1834
TseFI GTSAC 2 cut(s) 905, 1139
TseI GCWGC 2 cut(s) 305, 982
Tsp45I GTSAC 2 cut(s) 905, 1139
TspDTI ATGAA 7 cut(s) 50, 140, 813, 936, 954, 1324, 1551
TspRI CASTG 8 cut(s) 216, 295, 726, 757, 789, 1264, 1771, 1834
VneI GTGCAC 2 cut(s) 705, 1526
VpaK11BI GGWCC 1 cut(s) 1066
VspI ATTAAT 1 cut(s) 1668
XagI CCTNNNNNAGG 1 cut(s) 1152
XapI RAATTY 4 cut(s) 283, 313, 877, 1244
XceI RCATGY 5 cut(s) 89, 700, 1216, 1273, 1548
XcmI CCANNNNNNNNNTGG 1 cut(s) 1625
XmiI GTMKAC 1 cut(s) 838
XspI CTAG 4 cut(s) 378, 605, 800, 1572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.