Rh4CG384900

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0002060
Physical Location & Seq
Reverse (-)
63727466 .. 63731021
3556 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG384900.1

Sequence Viewer

Length: 1836 bp
ATGAAACAACTGAAAGTGATGAGGTTGTTAATTCTGTTGGGTCTTGTAATCATCCAAGAGGCAACTTCACATGGAGAGCACCCTCTTTCCAGAATTTCTATTCATCAAGCAACCTTTGCTCTTCATGACCTTGCTTATATCAAAGCCTCTCCTACTGTTCTAGGATTAAGGGGGCAAGACACTGAATGGGTGACTTTGGAATTTGGTTCTGAAAACCCTTCAACTGAGGATTGGATTGGAGTATTTTCTCCTGCTAATTTTAGTGCTTCTTCCTGCCCTGAGGAAAATCCAAGAGTTTATCCTCCATTTTTGTGCTCTGCACCTATTAAGTTTCAATATGCAAATTACTCCAGTCCCGAGTACAAGGACACTGGAAAAGGCTTCTTGAAGCTTCAGCTGATCAACCAGAGATCGGACTTCTCTTTCGCGCTGTTTTCTGGTGGTTTACTGAATCCAAAGGTCGTGGCGGTGTCAAATGTTGTAGCTTTCTCTAATCCAAATGCACCAGTTTATCCACGAATAGCACAAGGGAAAGAATGGAATGAAATGACTGTAACATGGACTAGTGGATATGGGATTGATGAGGCAGAGCCTTTTGTTGATTGGGGTCCAAGAGGAGAACGTTTGCGTTCCCCAGCTGTGACACAAACTTTTGATCGTCATAGCATGTGTGGTGCACCTGCAAGAACAGTGGGATGGCGTGACCCTGGATTCATACACACTAGTTTTCTGAAGGAGTTGTGGCCAAATAAAGTGTATACGTACAGGCTGGGACATAGATTGTTTAATGGTACATATATATGGAGTCAAGACTATCAGTTTAAAGCATCTCCTTATCCTGGTCAAAATTCTTTACAACGTGTAGTCATTTTCGGTGACATGGGAAAGGATGAAGCTGATGGTTCCAATGAATATAACAATTTCCAGAGAGGCTCTCTCAACACTACTAAGCAGCTTATTCGAGACCTGAAAAACACTGATATTGTGTTCCATATTGGAGATATATGTTATGCAAATGGTTACCTTTCACAATGGGACCAATTTACTGCACAAGTTGAGCCAATAGCATCAACTGTTCCTTACATGATTGCAAGTGGCAACCATGAACGCGACTGGCCAGGATCAGGTTCTTTCTATGGGAACATGGATTCAGGAGGAGAATGTGGTGTGTTGGCTGAAAATATGTTCTATGTTCCCACCAAGAACAATGCTAAGTTCTGGTACTCCACTGACTATGGCATGTTTCACTTCTGCATAGCGGACACAGAACATGATTGGAGAGAGGGAACAGAGCAGTACAAATTCATTGAGCACTGTTTAGCATCAGTCAATAGACAGAAGCAACCATGGCTAATTTTTCTTGCACATCGGGTTCTCGGATATTCTTCTTGTATCTCTTATGCAGAAGAAGGATCATTTAATGAACCAATGGGGAGGGAGAGCCTTCAGAAGCTATGGCAAAAGTACAAGGTTGACATTGCCATTTATGGCCATGTACATAACTATGAAAGAACATGTCCCATATACCAGAATATTTGCACAAATGAAGAGAAGCACTATTATAAGGGCAGCTTGAATGGAACAATACATGTGGTTGCTGGTGGTGCAGGAGCAAGTCTATCAACTTTTACATCCCTTCAAACAAAATGGAGTATTTTCAAAGACTATGACCATGGATTTGTCAAGCTCACAGCATTTGATCATTCAAATTTGTTGTTCGAGTACAAGAAGAGCAGGGATGGACAGGTTTATGACTCTTTCAGAATATCCAGAGATTATAGGGACATCTTGGCCTGCACAGTTGATAGTTGTCCAAGCACGACACTAGCGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

611

Amino Acids

69.3

Weight (kDa)

6.18

Isoelectric Point (pI)

35.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_PAP PF17808 47 - 165 1.5e-42 Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N PF16656 173 - 275 4.2e-11 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 287 - 502 1.9e-18 Calcineurin-like phosphoesterase
Metallophos_C PF14008 527 - 586 3.2e-15 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1563
AarI CACCTGC 1 cut(s) 688
AasI GACNNNNNNGTC 1 cut(s) 1828
Acc36I ACCTGC 1 cut(s) 688
AccI GTMKAC 1 cut(s) 758
AccII CGCG 2 cut(s) 428, 1110
AciI CCGC 2 cut(s) 467, 1259
AclI AACGTT 1 cut(s) 622
AclWI GGATC 2 cut(s) 1129, 1420
AcoI YGGCCR 3 cut(s) 743, 1115, 1489
AcsI RAATTY 5 cut(s) 93, 200, 847, 1301, 1708
AcuI CTGAAG 3 cut(s) 377, 752, 1430
AfaI GTAC 8 cut(s) 362, 764, 793, 1223, 1298, 1466, 1497, 1724
AfiI CCNNNNNNNGG 3 cut(s) 412, 839, 1124
AflIII ACRYGT 3 cut(s) 859, 1514, 1588
AgsI TTSAA 7 cut(s) 222, 335, 388, 1576, 1640, 1660, 1707
AhlI ACTAGT 2 cut(s) 563, 722
AjnI CCWGG 3 cut(s) 706, 838, 1117
AluBI AGCT 9 cut(s) 391, 397, 485, 638, 896, 955, 1453, 1572, 1687
AluI AGCT 9 cut(s) 391, 397, 485, 638, 896, 955, 1453, 1572, 1687
Alw21I GWGCWC 4 cut(s) 81, 317, 679, 1314
Alw26I GTCTC 1 cut(s) 957
Alw44I GTGCAC 1 cut(s) 675
AlwI GGATC 2 cut(s) 1129, 1420
Ama87I CYCGRG 1 cut(s) 356
AoxI GGCC 4 cut(s) 743, 1115, 1489, 1791
ApaLI GTGCAC 1 cut(s) 675
ApeKI GCWGC 2 cut(s) 952, 1569
ApoI RAATTY 5 cut(s) 93, 200, 847, 1301, 1708
ArsI GACNNNNNNTTYG 4 cut(s) 407, 439, 1661, 1693
AspLEI GCGC 1 cut(s) 430
AspS9I GGNCC 2 cut(s) 608, 1036
AsuHPI GGTGA 2 cut(s) 202, 887
AvaI CYCGRG 1 cut(s) 356
AvaII GGWCC 2 cut(s) 608, 1036
AxyI CCTNAGG 1 cut(s) 279
BaeGI GKGCMC 1 cut(s) 679
BalI TGGCCA 3 cut(s) 745, 1117, 1491
Bbv12I GWGCWC 4 cut(s) 81, 317, 679, 1314
BbvI GCAGC 2 cut(s) 964, 1581
BccI CCATC 3 cut(s) 690, 893, 1733
BcgI CGANNNNNNTGC 2 cut(s) 941, 975
BciT130I CCWGG 3 cut(s) 708, 840, 1119
BclI TGATCA 2 cut(s) 399, 1699
BcoDI GTCTC 1 cut(s) 957
BcuI ACTAGT 2 cut(s) 563, 722
BfaI CTAG 4 cut(s) 161, 564, 723, 1826
BfuAI ACCTGC 1 cut(s) 688
BisI GCNGC 2 cut(s) 953, 1570
BlsI GCNGC 2 cut(s) 954, 1571
Bme1390I CCNGG 3 cut(s) 708, 840, 1119
Bme18I GGWCC 2 cut(s) 608, 1036
BmeT110I CYCGRG 1 cut(s) 356
BmgT120I GGNCC 2 cut(s) 608, 1036
BmiI GGNNCC 3 cut(s) 609, 904, 1037
BmrFI CCNGG 3 cut(s) 708, 840, 1119
BmsI GCATC 3 cut(s) 836, 1076, 1331
BplI GAGNNNNNCTC 4 cut(s) 919, 951, 921, 953
BpmI CTGGAG 1 cut(s) 334
BsaAI YACGTR 1 cut(s) 762
BsaI GGTCTC 1 cut(s) 957
BsaJI CCNNGG 3 cut(s) 706, 1346, 1672
Bsc4I CCNNNNNNNGG 3 cut(s) 412, 839, 1124
Bse1I ACTGG 4 cut(s) 351, 376, 506, 1118
Bse21I CCTNAGG 1 cut(s) 279
Bse3DI GCAATG 1 cut(s) 1476
BseBI CCWGG 3 cut(s) 708, 840, 1119
BseDI CCNNGG 3 cut(s) 706, 1346, 1672
BseGI GGATG 5 cut(s) 51, 701, 895, 1631, 1744
BseLI CCNNNNNNNGG 3 cut(s) 412, 839, 1124
BseMI GCAATG 1 cut(s) 1476
BseMII CTCAG 2 cut(s) 216, 270
BseNI ACTGG 4 cut(s) 351, 376, 506, 1118
BseRI GAGGAG 2 cut(s) 630, 1170
BseSI GKGCMC 1 cut(s) 679
BseXI GCAGC 2 cut(s) 964, 1581
BseYI CCCAGC 2 cut(s) 634, 769
BsgI GTGCAG 4 cut(s) 303, 1032, 1626, 1780
Bsh1236I CGCG 2 cut(s) 428, 1110
BshFI GGCC 4 cut(s) 745, 1117, 1491, 1793
BsiHKAI GWGCWC 4 cut(s) 81, 317, 679, 1314
BsiHKCI CYCGRG 1 cut(s) 356
BslFI GGGAC 5 cut(s) 339, 786, 1049, 1503, 1796
BslI CCNNNNNNNGG 3 cut(s) 412, 839, 1124
BsmAI GTCTC 1 cut(s) 957
BsmFI GGGAC 5 cut(s) 339, 786, 1049, 1503, 1796
BsnI GGCC 4 cut(s) 745, 1117, 1491, 1793
Bso31I GGTCTC 1 cut(s) 957
BsoBI CYCGRG 1 cut(s) 356
Bsp1286I GDGCHC 4 cut(s) 81, 317, 679, 1314
Bsp1407I TGTACA 1 cut(s) 1495
Bsp143I GATC 6 cut(s) 399, 410, 655, 1121, 1412, 1699
Bsp19I CCATGG 2 cut(s) 1346, 1672
BspACI CCGC 2 cut(s) 467, 1259
BspANI GGCC 4 cut(s) 745, 1117, 1491, 1793
BspCNI CTCAG 2 cut(s) 217, 271
BspFNI CGCG 2 cut(s) 428, 1110
BspHI TCATGA 2 cut(s) 124, 1832
BspLI GGNNCC 3 cut(s) 609, 904, 1037
BspMI ACCTGC 1 cut(s) 688
BspPI GGATC 2 cut(s) 1129, 1420
BspQI GCTCTTC 2 cut(s) 126, 1724
BspTNI GGTCTC 1 cut(s) 957
BsrDI GCAATG 1 cut(s) 1476
BsrGI TGTACA 1 cut(s) 1495
BsrI ACTGG 4 cut(s) 351, 376, 506, 1118
BssECI CCNNGG 3 cut(s) 706, 1346, 1672
BssMI GATC 6 cut(s) 399, 410, 655, 1121, 1412, 1699
BssNAI GTATAC 1 cut(s) 759
BssT1I CCWWGG 2 cut(s) 1346, 1672
Bst1107I GTATAC 1 cut(s) 759
Bst2UI CCWGG 3 cut(s) 708, 840, 1119
Bst4CI ACNGT 6 cut(s) 157, 553, 691, 1075, 1316, 1801
Bst6I CTCTTC 3 cut(s) 126, 1542, 1724
BstAPI GCANNNNNTGC 1 cut(s) 116
BstAUI TGTACA 1 cut(s) 1495
BstBAI YACGTR 1 cut(s) 762
BstC8I GCNNGC 1 cut(s) 1795
BstDEI CTNAG 4 cut(s) 225, 279, 948, 1212
BstDSI CCRYGG 2 cut(s) 1346, 1672
BstEII GGTNACC 1 cut(s) 1019
BstF5I GGATG 5 cut(s) 51, 701, 895, 1631, 1744
BstFNI CGCG 2 cut(s) 428, 1110
BstHHI GCGC 1 cut(s) 430
BstKTI GATC 6 cut(s) 402, 413, 658, 1124, 1415, 1702
BstMAI GTCTC 1 cut(s) 957
BstMBI GATC 6 cut(s) 399, 410, 655, 1121, 1412, 1699
BstMWI GCNNNNNNNGC 3 cut(s) 116, 1348, 1604
BstNI CCWGG 3 cut(s) 708, 840, 1119
BstNSI RCATGY 4 cut(s) 670, 1243, 1518, 1592
BstPI GGTNACC 1 cut(s) 1019
BstSCI CCNGG 3 cut(s) 706, 838, 1117
BstSLI GKGCMC 1 cut(s) 679
BstSNI TACGTA 1 cut(s) 762
BstUI CGCG 2 cut(s) 428, 1110
BstV1I GCAGC 2 cut(s) 964, 1581
BstZ17I GTATAC 1 cut(s) 759
Bsu36I CCTNAGG 1 cut(s) 279
BsuRI GGCC 4 cut(s) 745, 1117, 1491, 1793
BtgI CCRYGG 2 cut(s) 1346, 1672
BtsCI GGATG 5 cut(s) 51, 701, 895, 1631, 1744
BtsIMutI CAGTG 6 cut(s) 180, 369, 696, 975, 1227, 1312
BveI ACCTGC 1 cut(s) 688
Cac8I GCNNGC 1 cut(s) 1795
CciI TCATGA 2 cut(s) 124, 1832
CfoI GCGC 1 cut(s) 430
Cfr13I GGNCC 2 cut(s) 608, 1036
CseI GACGC 1 cut(s) 1818
Csp6I GTAC 8 cut(s) 361, 763, 792, 1222, 1297, 1465, 1496, 1723
CspCI CAANNNNNGTGG 6 cut(s) 444, 479, 672, 707, 1572, 1607
CviQI GTAC 8 cut(s) 361, 763, 792, 1222, 1297, 1465, 1496, 1723
DdeI CTNAG 4 cut(s) 225, 279, 948, 1212
DpnI GATC 6 cut(s) 401, 412, 657, 1123, 1414, 1701
DpnII GATC 6 cut(s) 399, 410, 655, 1121, 1412, 1699
DraI TTTAAA 1 cut(s) 823
DrdI GACNNNNNNGTC 1 cut(s) 1828
DseDI GACNNNNNNGTC 1 cut(s) 1828
EaeI YGGCCR 3 cut(s) 743, 1115, 1489
Eam1104I CTCTTC 3 cut(s) 126, 1542, 1724
EarI CTCTTC 3 cut(s) 126, 1542, 1724
Eco105I TACGTA 1 cut(s) 762
Eco130I CCWWGG 2 cut(s) 1346, 1672
Eco31I GGTCTC 1 cut(s) 957
Eco47I GGWCC 2 cut(s) 608, 1036
Eco57I CTGAAG 3 cut(s) 377, 752, 1430
Eco81I CCTNAGG 1 cut(s) 279
Eco88I CYCGRG 1 cut(s) 356
Eco91I GGTNACC 1 cut(s) 1019
EcoO65I GGTNACC 1 cut(s) 1019
EcoRII CCWGG 3 cut(s) 706, 838, 1117
EcoT14I CCWWGG 2 cut(s) 1346, 1672
ErhI CCWWGG 2 cut(s) 1346, 1672
FalI AAGNNNNNCTT 2 cut(s) 1556, 1588
FaqI GGGAC 5 cut(s) 339, 786, 1049, 1503, 1796
FbaI TGATCA 2 cut(s) 399, 1699
FblI GTMKAC 1 cut(s) 758
Fnu4HI GCNGC 2 cut(s) 953, 1570
FokI GGATG 5 cut(s) 38, 708, 902, 1618, 1751
Fsp4HI GCNGC 2 cut(s) 953, 1570
FspBI CTAG 4 cut(s) 161, 564, 723, 1826
GlaI GCGC 1 cut(s) 429
GluI GCNGC 2 cut(s) 953, 1570
GsaI CCCAGC 2 cut(s) 638, 773
GsuI CTGGAG 1 cut(s) 334
HaeIII GGCC 4 cut(s) 745, 1117, 1491, 1793
HgaI GACGC 1 cut(s) 1818
HhaI GCGC 1 cut(s) 430
Hin6I GCGC 1 cut(s) 428
HinP1I GCGC 1 cut(s) 428
HincII GTYRAC 1 cut(s) 1474
HindII GTYRAC 1 cut(s) 1474
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 5 cut(s) 451, 711, 805, 1148, 1754
HphI GGTGA 2 cut(s) 202, 887
Hpy166II GTNNAC 4 cut(s) 446, 677, 759, 1474
Hpy188I TCNGA 6 cut(s) 211, 415, 732, 1379, 1449, 1763
Hpy8I GTNNAC 4 cut(s) 446, 677, 759, 1474
HpyAV CCTTC 5 cut(s) 228, 727, 1403, 1454, 1646
HpyCH4III ACNGT 6 cut(s) 157, 553, 691, 1075, 1316, 1801
HpyCH4IV ACGT 3 cut(s) 622, 761, 859
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 1348, 1604
HpyF3I CTNAG 4 cut(s) 225, 279, 948, 1212
HpySE526I ACGT 3 cut(s) 622, 761, 859
HspAI GCGC 1 cut(s) 428
Ksp22I TGATCA 2 cut(s) 399, 1699
Kzo9I GATC 6 cut(s) 399, 410, 655, 1121, 1412, 1699
LguI GCTCTTC 2 cut(s) 126, 1724
LmnI GCTCC 1 cut(s) 1610
Lsp1109I GCAGC 2 cut(s) 964, 1581
LweI GCATC 3 cut(s) 836, 1076, 1331
MaeI CTAG 4 cut(s) 161, 564, 723, 1826
MaeII ACGT 3 cut(s) 622, 761, 859
MaeIII GTNAC 6 cut(s) 190, 553, 640, 701, 875, 1019
MalI GATC 6 cut(s) 401, 412, 657, 1123, 1414, 1701
MboI GATC 6 cut(s) 399, 410, 655, 1121, 1412, 1699
MboII GAAGA 6 cut(s) 113, 261, 1377, 1418, 1559, 1741
MhlI GDGCHC 4 cut(s) 81, 317, 679, 1314
MlsI TGGCCA 3 cut(s) 745, 1117, 1491
MluNI TGGCCA 3 cut(s) 745, 1117, 1491
MlyI GAGTC 2 cut(s) 814, 1748
Mox20I TGGCCA 3 cut(s) 745, 1117, 1491
MscI TGGCCA 3 cut(s) 745, 1117, 1491
MseI TTAA 6 cut(s) 29, 167, 327, 786, 822, 1419
MslI CAYNNNNRTG 3 cut(s) 310, 799, 1503
Msp20I TGGCCA 3 cut(s) 745, 1117, 1491
MspA1I CMGCKG 2 cut(s) 397, 638
MspR9I CCNGG 3 cut(s) 708, 840, 1119
MvaI CCWGG 3 cut(s) 708, 840, 1119
MvnI CGCG 2 cut(s) 428, 1110
MwoI GCNNNNNNNGC 3 cut(s) 116, 1348, 1604
NcoI CCATGG 2 cut(s) 1346, 1672
NdeII GATC 6 cut(s) 399, 410, 655, 1121, 1412, 1699
NlaIV GGNNCC 3 cut(s) 609, 904, 1037
NmuCI GTSAC 4 cut(s) 190, 640, 701, 875
NspI RCATGY 4 cut(s) 670, 1243, 1518, 1592
PagI TCATGA 2 cut(s) 124, 1832
PaqCI CACCTGC 1 cut(s) 688
PciI ACATGT 2 cut(s) 1514, 1588
PciSI GCTCTTC 2 cut(s) 126, 1724
PcsI WCGNNNNNNNCGW 1 cut(s) 1826
PfeI GAWTC 3 cut(s) 451, 711, 1148
PkrI GCNGC 2 cut(s) 954, 1571
PleI GAGTC 2 cut(s) 813, 1748
PpsI GAGTC 2 cut(s) 813, 1748
Ppu21I YACGTR 1 cut(s) 762
PscI ACATGT 2 cut(s) 1514, 1588
PsiI TTATAA 1 cut(s) 1563
Psp1406I AACGTT 1 cut(s) 622
Psp6I CCWGG 3 cut(s) 706, 838, 1117
PspEI GGTNACC 1 cut(s) 1019
PspFI CCCAGC 2 cut(s) 634, 769
PspGI CCWGG 3 cut(s) 706, 838, 1117
PspN4I GGNNCC 3 cut(s) 609, 904, 1037
PspPI GGNCC 2 cut(s) 608, 1036
PvuII CAGCTG 2 cut(s) 397, 638
RsaI GTAC 8 cut(s) 362, 764, 793, 1223, 1298, 1466, 1497, 1724
RsaNI GTAC 8 cut(s) 361, 763, 792, 1222, 1297, 1465, 1496, 1723
RseI CAYNNNNRTG 3 cut(s) 310, 799, 1503
SapI GCTCTTC 2 cut(s) 126, 1724
SaqAI TTAA 6 cut(s) 29, 167, 327, 786, 822, 1419
SatI GCNGC 2 cut(s) 953, 1570
Sau3AI GATC 6 cut(s) 399, 410, 655, 1121, 1412, 1699
Sau96I GGNCC 2 cut(s) 608, 1036
SchI GAGTC 2 cut(s) 814, 1748
ScrFI CCNGG 3 cut(s) 708, 840, 1119
SduI GDGCHC 4 cut(s) 81, 317, 679, 1314
SfaNI GCATC 3 cut(s) 836, 1076, 1331
SinI GGWCC 2 cut(s) 608, 1036
SmiMI CAYNNNNRTG 3 cut(s) 310, 799, 1503
SnaBI TACGTA 1 cut(s) 762
SpeI ACTAGT 2 cut(s) 563, 722
SsiI CCGC 2 cut(s) 467, 1259
SspI AATATT 1 cut(s) 1534
SspMI CTAG 4 cut(s) 161, 564, 723, 1826
StyD4I CCNGG 3 cut(s) 706, 838, 1117
StyI CCWWGG 2 cut(s) 1346, 1672
TaaI ACNGT 6 cut(s) 157, 553, 691, 1075, 1316, 1801
TaiI ACGT 3 cut(s) 625, 764, 862
TaqI TCGA 2 cut(s) 961, 1719
TatI WGTACW 5 cut(s) 360, 1296, 1464, 1495, 1722
TfiI GAWTC 3 cut(s) 451, 711, 1148
Tru1I TTAA 6 cut(s) 29, 167, 327, 786, 822, 1419
Tru9I TTAA 6 cut(s) 29, 167, 327, 786, 822, 1419
TscAI CASTG 6 cut(s) 187, 376, 696, 982, 1234, 1319
TseFI GTSAC 4 cut(s) 190, 640, 701, 875
TseI GCWGC 2 cut(s) 952, 1569
Tsp45I GTSAC 4 cut(s) 190, 640, 701, 875
TspRI CASTG 6 cut(s) 187, 376, 696, 982, 1234, 1319
VneI GTGCAC 1 cut(s) 675
VpaK11BI GGWCC 2 cut(s) 608, 1036
XapI RAATTY 5 cut(s) 93, 200, 847, 1301, 1708
XceI RCATGY 4 cut(s) 670, 1243, 1518, 1592
XmiI GTMKAC 1 cut(s) 758
XspI CTAG 4 cut(s) 161, 564, 723, 1826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.