Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0011918

Physical Location & Seq

Genomic Coordinates Forward (+)

6084709 .. 6086425

Gene Structure 1717 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1088200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1308 bp

ATGGCAAGCCCTTTCAAAGACCAATCTCATCACTCATTATCTCTGGCCAAAAAGCTCTTGCCTTATGCAATCTTTGCCCTCCTCCCCATTGCTGTAATCCGCTTGTACTTATACACTCCCTCTCTCCCTCTCACCTCCACAGATCACCTCCCTCACTCCAGAGAAATTACCATCTCAACCTCCTCTCCTACTCCATTACCCCGTATTTCCTCCTCTCAAGAAAAGGCTAAAGAAACTACATCTACATGTGACTACACCAGGGGCAAATGGGTCCATGAAAAGCTGGGTCCTTTGTACAATGGCACAACCTGTGGGACAATCAAGGAAGGTCAGAACTGCATCACTCATGGAAGAACTGATTTGGGTTATCTCTACTGGAGGTGGAAACCCAGCAAGTGCCAGCTTCCCAGGTTTGAACCCAGCACTTTTCTCCAACTCATTAGCAACAAGCACATAGCTTTTGTGGGGGACTCCATGGCCAGGAACCAGTTGGAGTCCCTCCTTTGCCTGCTTGCCACTGCCTCTCCTCCCAACCTTGTTTACACAGATGGGGAGGAGAGGAAGTTTAGGAGATGGAATTTCCCGTCCCACAATGCCAATGTGTCGGTTTATTGGTCACCATTTCTTGTGAAAGGGGTTGAGAAATCACCAAATGGTCCAAATTATAATAAATTGTATTTGGATCAAGTCGATGAGAGGTGGGCTGCAGATTTGGGGGGATTTGATATGTTTGTATTGTCAGTTGGGCATTGGTTTTTGCACCCAGCAGTGTATTTTGAGGGGGATGATGTTCTGGGGTGCCATTTTTGCCCTGGATTAAACCACACTGAGATTGGATTCTATGATGTGTTGAGGAAAGCTGTGAGGACTACTCTCAATACCATAATTGAGAGGAGAGGGAATGGGAATGGGATTGATGTGGTTCTGACGACATTTTCGCCTGCGCATTTTGAAGGGGAATGGGACAAGATTGGGGCTTGTTCAAAGAACAAACCCTACAAGGAAGGGGAGAAGCAGCTTGAAGGAATGGATGCCGAGATGAGGCAGCTTGAGGTGGAAGAAGTGGAAGTGGCTAGGGCAAATGGGAAGAAATTCAAAGGGTTTAGGCTGGAGGCATTGGATGTGACAAAATTGTCCTTGATGAGGCCAGATGGGCATCCAGGGCCTTACATGAATAAGTTTCCATTTGCTGATGGGGTTCCTGAGAGGGTGCAGAATGATTGTGTGCATTGGTGCTTGCCAGGGCCCATTGACACATGGAATGAGATTCTGCTGGAGGTTATGAAGAAATGGAAACAAAGGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005975 GO:0005976 GO:0006073 GO:0008150 GO:0008152 GO:0008374 GO:0009987 GO:0010383 GO:0010410 GO:0010411 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0071554 GO:0071704

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

436

Amino Acids

49.41

Weight (kDa)

8.22

Isoelectric Point (pI)

42.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	82 - 134	3.2e-20	PMR5 N terminal Domain
PC-Esterase	PF13839	135 - 426	1.6e-92	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011918)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70230
fragaria_vesca	FvH4_4g34920
malus_domestica	MD13G1087900.v1.1 MD16G1088200.v1.1
prunus_persica	Prupe.1G258400_v2.0.a1
pyrus_communis	pycom13g07560 pycom16g07550
rosa_chinensis	RchiOBHm_Chr4g0444361
rosa_laevigata	RLG00000005837
rosa_multiflora	Rmu_sc0002841.1_g000009
rosa_roxburghii	Rroxscaffold_5G00384900
rosa_rugosa	Rorug04G0352100 Rorug04G0352200 Rorug04G0352200
rosa_samantha	Rh4AG412600 Rh4BG424100 Rh4CG438400 Rh4DG419300
rosa_wichuraiana	Rw4G035440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	666
AasI	GACNNNNNNGTC	1 cut(s)	1132
Acc16I	TGCGCA	1 cut(s)	945
AccB1I	GGYRCC	1 cut(s)	798
AciI	CCGC	1 cut(s)	100
AclWI	GGATC	1 cut(s)	690
AcoI	YGGCCR	2 cut(s)	45, 477
AcsI	RAATTY	2 cut(s)	577, 1091
AfaI	GTAC	2 cut(s)	107, 296
AfiI	CCNNNNNNNGG	3 cut(s)	480, 1041, 1143
AflIII	ACRYGT	1 cut(s)	245
AgsI	TTSAA	6 cut(s)	16, 416, 953, 984, 1022, 1096
AjnI	CCWGG	6 cut(s)	257, 407, 479, 811, 1159, 1240
AluBI	AGCT	7 cut(s)	55, 283, 403, 458, 860, 1018, 1048
AluI	AGCT	7 cut(s)	55, 283, 403, 458, 860, 1018, 1048
AlwI	GGATC	1 cut(s)	690
AoxI	GGCC	5 cut(s)	45, 477, 1145, 1163, 1244
ApaI	GGGCCC	1 cut(s)	1248
ApeKI	GCWGC	3 cut(s)	704, 1015, 1045
ApoI	RAATTY	2 cut(s)	577, 1091
ArsI	GACNNNNNNTTYG	2 cut(s)	919, 951
Asp700I	GAANNNNTTC	1 cut(s)	1091
AspLEI	GCGC	1 cut(s)	946
AspS9I	GGNCC	6 cut(s)	271, 287, 656, 1163, 1244, 1245
AsuHPI	GGTGA	4 cut(s)	124, 137, 609, 639
AvaII	GGWCC	3 cut(s)	271, 287, 656
BaeGI	GKGCMC	1 cut(s)	1248
BalI	TGGCCA	2 cut(s)	47, 479
BanI	GGYRCC	1 cut(s)	798
BanII	GRGCYC	1 cut(s)	1248
BbvI	GCAGC	3 cut(s)	691, 1027, 1057
BccI	CCATC	5 cut(s)	179, 542, 567, 1145, 1187
BciT130I	CCWGG	6 cut(s)	259, 409, 481, 813, 1161, 1242
BfaI	CTAG	1 cut(s)	1074
BfmI	CTRYAG	1 cut(s)	705
BglI	GCCNNNNNGGC	1 cut(s)	1153
BisI	GCNGC	3 cut(s)	705, 1016, 1046
BlsI	GCNGC	3 cut(s)	706, 1017, 1047
Bme1390I	CCNGG	6 cut(s)	259, 409, 481, 813, 1161, 1242
Bme18I	GGWCC	3 cut(s)	271, 287, 656
BmgT120I	GGNCC	6 cut(s)	271, 287, 656, 1163, 1244, 1245
BmiI	GGNNCC	6 cut(s)	272, 288, 485, 800, 1200, 1246
BmrFI	CCNGG	6 cut(s)	259, 409, 481, 813, 1161, 1242
BmsI	GCATC	3 cut(s)	348, 1021, 1165
BplI	GAGNNNNNCTC	2 cut(s)	856, 888
BpmI	CTGGAG	4 cut(s)	142, 397, 1130, 1295
BpuEI	CTTGAG	2 cut(s)	201, 1070
BsaBI	GATNNNNATC	1 cut(s)	1155
BsaJI	CCNNGG	6 cut(s)	258, 407, 474, 811, 1160, 1241
BsaXI	ACNNNNNCTCC	6 cut(s)	169, 199, 508, 538, 548, 578
Bsc4I	CCNNNNNNNGG	3 cut(s)	480, 1041, 1143
Bse1I	ACTGG	2 cut(s)	380, 487
Bse3DI	GCAATG	1 cut(s)	87
Bse8I	GATNNNNATC	1 cut(s)	1155
BseBI	CCWGG	6 cut(s)	259, 409, 481, 813, 1161, 1242
BseDI	CCNNGG	6 cut(s)	258, 407, 474, 811, 1160, 1241
BseGI	GGATG	4 cut(s)	790, 1036, 1126, 1156
BseJI	GATNNNNATC	1 cut(s)	1155
BseLI	CCNNNNNNNGG	3 cut(s)	480, 1041, 1143
BseMI	GCAATG	1 cut(s)	87
BseMII	CTCAG	2 cut(s)	819, 1194
BseNI	ACTGG	2 cut(s)	380, 487
BseRI	GAGGAG	6 cut(s)	71, 172, 202, 516, 569, 907
BseSI	GKGCMC	1 cut(s)	1248
BseXI	GCAGC	3 cut(s)	691, 1027, 1057
BseYI	CCCAGC	4 cut(s)	283, 389, 419, 763
BsgI	GTGCAG	1 cut(s)	1232
BshFI	GGCC	5 cut(s)	47, 479, 1147, 1165, 1246
BshNI	GGYRCC	1 cut(s)	798
BslFI	GGGAC	5 cut(s)	328, 481, 482, 571, 977
BslI	CCNNNNNNNGG	3 cut(s)	480, 1041, 1143
BsmFI	GGGAC	5 cut(s)	328, 481, 482, 571, 977
BsnI	GGCC	5 cut(s)	47, 479, 1147, 1165, 1246
Bsp120I	GGGCCC	1 cut(s)	1244
Bsp1286I	GDGCHC	1 cut(s)	1248
Bsp1407I	TGTACA	1 cut(s)	294
Bsp143I	GATC	2 cut(s)	142, 682
Bsp19I	CCATGG	1 cut(s)	474
BspACI	CCGC	1 cut(s)	100
BspANI	GGCC	5 cut(s)	47, 479, 1147, 1165, 1246
BspCNI	CTCAG	2 cut(s)	820, 1195
BspLI	GGNNCC	6 cut(s)	272, 288, 485, 800, 1200, 1246
BspMAI	CTGCAG	1 cut(s)	709
BspPI	GGATC	1 cut(s)	690
BspT107I	GGYRCC	1 cut(s)	798
BsrDI	GCAATG	1 cut(s)	87
BsrGI	TGTACA	1 cut(s)	294
BsrI	ACTGG	2 cut(s)	380, 487
BssECI	CCNNGG	6 cut(s)	258, 407, 474, 811, 1160, 1241
BssMI	GATC	2 cut(s)	142, 682
BssT1I	CCWWGG	1 cut(s)	474
Bst2UI	CCWGG	6 cut(s)	259, 409, 481, 813, 1161, 1242
BstAPI	GCANNNNNTGC	1 cut(s)	74
BstAUI	TGTACA	1 cut(s)	294
BstC8I	GCNNGC	6 cut(s)	7, 401, 509, 513, 942, 1238
BstDEI	CTNAG	2 cut(s)	828, 1203
BstDSI	CCRYGG	1 cut(s)	474
BstEII	GGTNACC	1 cut(s)	615
BstENI	CCTNNNNNAGG	1 cut(s)	1141
BstF5I	GGATG	4 cut(s)	790, 1036, 1126, 1156
BstHHI	GCGC	1 cut(s)	946
BstKTI	GATC	2 cut(s)	145, 685
BstMBI	GATC	2 cut(s)	142, 682
BstMWI	GCNNNNNNNGC	4 cut(s)	74, 807, 1153, 1162
BstNI	CCWGG	6 cut(s)	259, 409, 481, 813, 1161, 1242
BstNSI	RCATGY	1 cut(s)	249
BstPI	GGTNACC	1 cut(s)	615
BstSCI	CCNGG	6 cut(s)	257, 407, 479, 811, 1159, 1240
BstSFI	CTRYAG	1 cut(s)	705
BstSLI	GKGCMC	1 cut(s)	1248
BstV1I	GCAGC	3 cut(s)	691, 1027, 1057
BsuRI	GGCC	5 cut(s)	47, 479, 1147, 1165, 1246
BtgI	CCRYGG	1 cut(s)	474
BtsCI	GGATG	4 cut(s)	790, 1036, 1126, 1156
BtsI	GCAGTG	2 cut(s)	516, 774
BtsIMutI	CAGTG	3 cut(s)	516, 774, 825
Cac8I	GCNNGC	6 cut(s)	7, 401, 509, 513, 942, 1238
CfoI	GCGC	1 cut(s)	946
Cfr13I	GGNCC	6 cut(s)	271, 287, 656, 1163, 1244, 1245
Csp6I	GTAC	2 cut(s)	106, 295
CviAII	CATG	6 cut(s)	246, 275, 347, 475, 1171, 1257
CviQI	GTAC	2 cut(s)	106, 295
DdeI	CTNAG	2 cut(s)	828, 1203
DpnI	GATC	2 cut(s)	144, 684
DpnII	GATC	2 cut(s)	142, 682
DrdI	GACNNNNNNGTC	1 cut(s)	1132
DseDI	GACNNNNNNGTC	1 cut(s)	1132
EaeI	YGGCCR	2 cut(s)	45, 477
Eco130I	CCWWGG	1 cut(s)	474
Eco24I	GRGCYC	1 cut(s)	1248
Eco47I	GGWCC	3 cut(s)	271, 287, 656
Eco91I	GGTNACC	1 cut(s)	615
EcoNI	CCTNNNNNAGG	1 cut(s)	1141
EcoO109I	RGGNCCY	3 cut(s)	287, 1163, 1244
EcoO65I	GGTNACC	1 cut(s)	615
EcoRII	CCWGG	6 cut(s)	257, 407, 479, 811, 1159, 1240
EcoT14I	CCWWGG	1 cut(s)	474
EcoT38I	GRGCYC	1 cut(s)	1248
ErhI	CCWWGG	1 cut(s)	474
FaeI	CATG	6 cut(s)	249, 278, 350, 478, 1174, 1260
FaqI	GGGAC	5 cut(s)	328, 481, 482, 571, 977
FatI	CATG	6 cut(s)	245, 274, 346, 474, 1170, 1256
Fnu4HI	GCNGC	3 cut(s)	705, 1016, 1046
FokI	GGATG	4 cut(s)	797, 1043, 1133, 1143
FriOI	GRGCYC	1 cut(s)	1248
Fsp4HI	GCNGC	3 cut(s)	705, 1016, 1046
FspBI	CTAG	1 cut(s)	1074
FspI	TGCGCA	1 cut(s)	945
GlaI	GCGC	1 cut(s)	945
GluI	GCNGC	3 cut(s)	705, 1016, 1046
GsaI	CCCAGC	4 cut(s)	287, 393, 423, 767
GsuI	CTGGAG	4 cut(s)	142, 397, 1130, 1295
HaeIII	GGCC	5 cut(s)	47, 479, 1147, 1165, 1246
HhaI	GCGC	1 cut(s)	946
Hin1II	CATG	6 cut(s)	249, 278, 350, 478, 1174, 1260
Hin6I	GCGC	1 cut(s)	944
HinP1I	GCGC	1 cut(s)	944
HinfI	GANTC	4 cut(s)	470, 494, 837, 1267
HphI	GGTGA	4 cut(s)	124, 137, 609, 639
Hpy166II	GTNNAC	1 cut(s)	541
Hpy188I	TCNGA	2 cut(s)	333, 927
Hpy188III	TCNNGA	3 cut(s)	159, 218, 1202
Hpy8I	GTNNAC	1 cut(s)	541
HpyAV	CCTTC	4 cut(s)	320, 947, 998, 1016
HpyCH4V	TGCA	6 cut(s)	68, 339, 707, 760, 1213, 1228
HpyF10VI	GCNNNNNNNGC	4 cut(s)	74, 807, 1153, 1162
HpyF3I	CTNAG	2 cut(s)	828, 1203
Hsp92II	CATG	6 cut(s)	249, 278, 350, 478, 1174, 1260
HspAI	GCGC	1 cut(s)	944
Kzo9I	GATC	2 cut(s)	142, 682
Lsp1109I	GCAGC	3 cut(s)	691, 1027, 1057
LweI	GCATC	3 cut(s)	348, 1021, 1165
MaeI	CTAG	1 cut(s)	1074
MaeIII	GTNAC	3 cut(s)	248, 615, 1123
MalI	GATC	2 cut(s)	144, 684
MboI	GATC	2 cut(s)	142, 682
MboII	GAAGA	4 cut(s)	363, 1070, 1099, 1297
MhlI	GDGCHC	1 cut(s)	1248
MlsI	TGGCCA	2 cut(s)	47, 479
MluCI	AATT	7 cut(s)	165, 577, 661, 671, 885, 1091, 1130
MluNI	TGGCCA	2 cut(s)	47, 479
MlyI	GAGTC	2 cut(s)	464, 503
MmeI	TCCRAC	2 cut(s)	457, 471
Mox20I	TGGCCA	2 cut(s)	47, 479
MroXI	GAANNNNTTC	1 cut(s)	1091
MscI	TGGCCA	2 cut(s)	47, 479
MseI	TTAA	1 cut(s)	818
MslI	CAYNNNNRTG	1 cut(s)	244
Msp20I	TGGCCA	2 cut(s)	47, 479
MspR9I	CCNGG	6 cut(s)	259, 409, 481, 813, 1161, 1242
MvaI	CCWGG	6 cut(s)	259, 409, 481, 813, 1161, 1242
MwoI	GCNNNNNNNGC	4 cut(s)	74, 807, 1153, 1162
NcoI	CCATGG	1 cut(s)	474
NdeII	GATC	2 cut(s)	142, 682
NlaIII	CATG	6 cut(s)	249, 278, 350, 478, 1174, 1260
NlaIV	GGNNCC	6 cut(s)	272, 288, 485, 800, 1200, 1246
NmeAIII	GCCGAG	1 cut(s)	1060
NmuCI	GTSAC	3 cut(s)	248, 615, 1123
NsbI	TGCGCA	1 cut(s)	945
NspI	RCATGY	1 cut(s)	249
PciI	ACATGT	1 cut(s)	245
PdmI	GAANNNNTTC	1 cut(s)	1091
PfeI	GAWTC	2 cut(s)	837, 1267
PkrI	GCNGC	3 cut(s)	706, 1017, 1047
PleI	GAGTC	2 cut(s)	464, 502
PpsI	GAGTC	2 cut(s)	464, 502
PpuMI	RGGWCCY	1 cut(s)	287
PscI	ACATGT	1 cut(s)	245
PsiI	TTATAA	1 cut(s)	666
Psp5II	RGGWCCY	1 cut(s)	287
Psp6I	CCWGG	6 cut(s)	257, 407, 479, 811, 1159, 1240
PspEI	GGTNACC	1 cut(s)	615
PspFI	CCCAGC	4 cut(s)	283, 389, 419, 763
PspGI	CCWGG	6 cut(s)	257, 407, 479, 811, 1159, 1240
PspN4I	GGNNCC	6 cut(s)	272, 288, 485, 800, 1200, 1246
PspOMI	GGGCCC	1 cut(s)	1244
PspPI	GGNCC	6 cut(s)	271, 287, 656, 1163, 1244, 1245
PspPPI	RGGWCCY	1 cut(s)	287
PsrI	GAACNNNNNNTAC	2 cut(s)	980, 1012
PstI	CTGCAG	1 cut(s)	709
RsaI	GTAC	2 cut(s)	107, 296
RsaNI	GTAC	2 cut(s)	106, 295
RseI	CAYNNNNRTG	1 cut(s)	244
SaqAI	TTAA	1 cut(s)	818
SatI	GCNGC	3 cut(s)	705, 1016, 1046
Sau3AI	GATC	2 cut(s)	142, 682
Sau96I	GGNCC	6 cut(s)	271, 287, 656, 1163, 1244, 1245
SchI	GAGTC	2 cut(s)	464, 503
ScrFI	CCNGG	6 cut(s)	259, 409, 481, 813, 1161, 1242
SduI	GDGCHC	1 cut(s)	1248
SfaNI	GCATC	3 cut(s)	348, 1021, 1165
SfcI	CTRYAG	1 cut(s)	705
SinI	GGWCC	3 cut(s)	271, 287, 656
SmiMI	CAYNNNNRTG	1 cut(s)	244
SmlI	CTYRAG	2 cut(s)	216, 1049
SmoI	CTYRAG	2 cut(s)	216, 1049
Sse9I	AATT	7 cut(s)	165, 577, 661, 671, 885, 1091, 1130
SsiI	CCGC	1 cut(s)	100
SspMI	CTAG	1 cut(s)	1074
StyD4I	CCNGG	6 cut(s)	257, 407, 479, 811, 1159, 1240
StyI	CCWWGG	1 cut(s)	474
TaqI	TCGA	1 cut(s)	690
TasI	AATT	7 cut(s)	165, 577, 661, 671, 885, 1091, 1130
TatI	WGTACW	2 cut(s)	105, 294
TfiI	GAWTC	2 cut(s)	837, 1267
Tru1I	TTAA	1 cut(s)	818
Tru9I	TTAA	1 cut(s)	818
TscAI	CASTG	3 cut(s)	523, 774, 832
TseFI	GTSAC	3 cut(s)	248, 615, 1123
TseI	GCWGC	3 cut(s)	704, 1015, 1045
Tsp45I	GTSAC	3 cut(s)	248, 615, 1123
TspDTI	ATGAA	3 cut(s)	291, 1187, 1298
TspRI	CASTG	3 cut(s)	523, 774, 832
VpaK11BI	GGWCC	3 cut(s)	271, 287, 656
XagI	CCTNNNNNAGG	1 cut(s)	1141
XapI	RAATTY	2 cut(s)	577, 1091
XceI	RCATGY	1 cut(s)	249
XcmI	CCANNNNNNNNNTGG	3 cut(s)	487, 809, 830
XmnI	GAANNNNTTC	1 cut(s)	1091
XspI	CTAG	1 cut(s)	1074

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.