RLG00000005837

Protein ALTERED XYLOGLUCAN

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0011918
Physical Location & Seq
Forward (+)
2271018 .. 2272532
1515 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005837

Sequence Viewer

Length: 1380 bp
ATGGTTAGTCCATTCAAAGACCACCAATCTCACTCTCCATCTTACACTCACTCCTTCATCAAGAAAATCTTACCTTATTTCATCTATGTCATACTTCCCATTGCTGTAATCCGCCTCTACCTTTACTCCCCCACTCTCCCTCAATCCTCCACAGTTCACTATCACCTCCCTCAACCCTCCACAGTTCATCACCTCCCTCACTCCACCACCCCCATTTCCATAACCACTCCATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCTTCACATGAAGAAGAAGAGAAGAAGCCAGAAGCTAAAGAAACTCAATGTGACTACACAGATGGGAACTGGGTCCCTGACAAAATGGGTCCTTTGTACAATGGCACAACCTGTGGGACAATTAAGGAAGGCCAGAATTGCATCACCCATGGGAGATCAGATTTGGGTTATCTCTACTGGAAATGGAAACCCAAGCAGTGCCCTTTACCAAGGTTTGAACCCAACACTTTTCTCCACCTCCTTAGAAACAAGCACATAGCTTTTGTCGGTGACTCCATGGCCAGGAACCAATTGGAGTCCCTTCTTTGCATGCTAGCCACTGCCTCTCCTCCCAAACTTGTTTACACTGATGGTGAGGACAACAAGTTTAGGAGGTGGAACTTTGCTTCTCACAATGTCAATGTGTCAGTGTATTGGTCACCCTTTCTTGTTAAAGGTGTTGAGAAATCAAAATCAGCTGATCACAATGAATTGTATTTGGATCAAATTGATGAGAGGTGGGCAGCTGATATGGGTAAGATGGACATGGTTGTGTTATCAGTTGGGCATTGGTTTTTGCATCCTGCCGTGTATTTTGAGGGTGGTTCAGCTCTTGGGTGTCATTTCTGTCCTGGTCTGAATCACACTGAGATTGGCTTCTATGATGTTCTGAGAAAGGCCGTAAGGACTACCCTAAAGACCATAACTGAAAGGAGAGGGACTAGTGAGAATGGGATCAATGTAATTGTGACTACATTTTCACCAGCTCATTTTGAAGGTGAGTGGGACAAGGCTGGGGCTTGTCCCAAGACCAAGCCTTACAACTATGGAGAGAAAAATCTTGAAGGAATGGATGCTGAGATGAGGCAAGTTGAGGTGGAAGAAGTGGAAGCTGCTAAGGTGAATGGTAAAGGGTTTTCAGGGTTTAAGGTTGAAGAATTGGATGTGAGCTATTTGTCATTGATGAGGCCAGATGGGCACCCTGGGCCTTATATGAACCCATTTCCATTTTTTAATGGGGTGAAAGAGAAGGTGCAAAATGATTGTGTACATTGGTGTTTGCCTGGGGCTATTGACACATGGAATGAGATATTGCTGGAGATCATGAAGAAATGGAATCGGGGTTGA

Protein Analysis

460

Amino Acids

51.86

Weight (kDa)

6.49

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 107 - 158 3.2e-20 PMR5 N terminal Domain
PC-Esterase PF13839 160 - 451 3.7e-92 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011918)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70230
fragaria_vesca FvH4_4g34920
malus_domestica MD13G1087900.v1.1 MD16G1088200.v1.1
prunus_persica Prupe.1G258400_v2.0.a1
pyrus_communis pycom13g07560 pycom16g07550
rosa_chinensis RchiOBHm_Chr4g0444361
rosa_laevigata RLG00000005837
rosa_multiflora Rmu_sc0002841.1_g000009
rosa_roxburghii Rroxscaffold_5G00384900
rosa_rugosa Rorug04G0352100 Rorug04G0352200 Rorug04G0352200
rosa_samantha Rh4AG412600 Rh4BG424100 Rh4CG438400 Rh4DG419300
rosa_wichuraiana Rw4G035440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1230
AciI CCGC 1 cut(s) 112
AclWI GGATC 2 cut(s) 762, 995
AcoI YGGCCR 1 cut(s) 552
AfaI GTAC 2 cut(s) 371, 1302
AfiI CCNNNNNNNGG 1 cut(s) 555
AgsI TTSAA 5 cut(s) 16, 491, 1028, 1097, 1187
AhlI ACTAGT 1 cut(s) 974
AjnI CCWGG 4 cut(s) 554, 883, 1234, 1315
AluBI AGCT 8 cut(s) 308, 533, 731, 779, 863, 1019, 1145, 1203
AluI AGCT 8 cut(s) 308, 533, 731, 779, 863, 1019, 1145, 1203
AlwI GGATC 2 cut(s) 762, 995
AoxI GGCC 5 cut(s) 403, 552, 930, 1220, 1238
ApeKI GCWGC 2 cut(s) 776, 1145
AspS9I GGNCC 3 cut(s) 346, 362, 1238
AsuNHI GCTAGC 1 cut(s) 586
AvaII GGWCC 2 cut(s) 346, 362
BaeGI GKGCMC 2 cut(s) 476, 1233
BalI TGGCCA 1 cut(s) 554
BanI GGYRCC 1 cut(s) 1230
BbvI GCAGC 2 cut(s) 788, 1132
BccI CCATC 6 cut(s) 46, 238, 329, 617, 787, 1220
BceAI ACGGC 2 cut(s) 824, 917
BciT130I CCWGG 4 cut(s) 556, 885, 1236, 1317
BclI TGATCA 1 cut(s) 733
BcuI ACTAGT 1 cut(s) 974
BfaI CTAG 2 cut(s) 587, 975
BglI GCCNNNNNGGC 1 cut(s) 1228
BisI GCNGC 2 cut(s) 777, 1146
BlsI GCNGC 2 cut(s) 778, 1147
Bme1390I CCNGG 4 cut(s) 556, 885, 1236, 1317
Bme18I GGWCC 2 cut(s) 346, 362
BmgT120I GGNCC 3 cut(s) 346, 362, 1238
BmiI GGNNCC 5 cut(s) 347, 348, 363, 560, 1232
BmrFI CCNGG 4 cut(s) 556, 885, 1236, 1317
BmrI ACTGGG 1 cut(s) 352
BmsI GCATC 3 cut(s) 423, 841, 1096
BmtI GCTAGC 1 cut(s) 590
BmuI ACTGGG 1 cut(s) 352
BpmI CTGGAG 1 cut(s) 1370
Bpu10I CCTNAGC 1 cut(s) 1149
BsaJI CCNNGG 6 cut(s) 421, 482, 549, 1234, 1235, 1316
BsaXI ACNNNNNCTCC 6 cut(s) 35, 65, 110, 140, 583, 613
Bsc4I CCNNNNNNNGG 1 cut(s) 555
Bse1I ACTGG 2 cut(s) 347, 455
Bse3DI GCAATG 1 cut(s) 99
BseBI CCWGG 4 cut(s) 556, 885, 1236, 1317
BseDI CCNNGG 6 cut(s) 421, 482, 549, 1234, 1235, 1316
BseGI GGATG 3 cut(s) 832, 1111, 1201
BseLI CCNNNNNNNGG 1 cut(s) 555
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 3 cut(s) 891, 914, 1101
BseNI ACTGG 2 cut(s) 347, 455
BseRI GAGGAG 1 cut(s) 591
BseSI GKGCMC 2 cut(s) 476, 1233
BseXI GCAGC 2 cut(s) 788, 1132
BseYI CCCAGC 1 cut(s) 1046
BshFI GGCC 5 cut(s) 405, 554, 932, 1222, 1240
BshNI GGYRCC 1 cut(s) 1230
BslFI GGGAC 6 cut(s) 332, 403, 556, 985, 1041, 1052
BslI CCNNNNNNNGG 1 cut(s) 555
BsmFI GGGAC 6 cut(s) 332, 403, 556, 985, 1041, 1052
BsnI GGCC 5 cut(s) 405, 554, 932, 1222, 1240
Bsp1286I GDGCHC 2 cut(s) 476, 1233
Bsp1407I TGTACA 2 cut(s) 369, 1300
Bsp143I GATC 5 cut(s) 428, 733, 754, 987, 1353
Bsp19I CCATGG 2 cut(s) 421, 549
BspACI CCGC 1 cut(s) 112
BspANI GGCC 5 cut(s) 405, 554, 932, 1222, 1240
BspCNI CTCAG 3 cut(s) 892, 915, 1102
BspHI TCATGA 1 cut(s) 1356
BspLI GGNNCC 5 cut(s) 347, 348, 363, 560, 1232
BspOI GCTAGC 1 cut(s) 590
BspPI GGATC 2 cut(s) 762, 995
BspT107I GGYRCC 1 cut(s) 1230
BsrDI GCAATG 1 cut(s) 99
BsrGI TGTACA 2 cut(s) 369, 1300
BsrI ACTGG 2 cut(s) 347, 455
BssECI CCNNGG 6 cut(s) 421, 482, 549, 1234, 1235, 1316
BssMI GATC 5 cut(s) 428, 733, 754, 987, 1353
BssT1I CCWWGG 3 cut(s) 421, 482, 549
Bst2UI CCWGG 4 cut(s) 556, 885, 1236, 1317
Bst4CI ACNGT 2 cut(s) 154, 184
Bst6I CTCTTC 2 cut(s) 280, 285
BstAUI TGTACA 2 cut(s) 369, 1300
BstC8I GCNNGC 2 cut(s) 584, 588
BstDEI CTNAG 5 cut(s) 515, 900, 923, 1110, 1149
BstDSI CCRYGG 2 cut(s) 421, 549
BstEII GGTNACC 1 cut(s) 690
BstF5I GGATG 3 cut(s) 832, 1111, 1201
BstKTI GATC 5 cut(s) 431, 736, 757, 990, 1356
BstMBI GATC 5 cut(s) 428, 733, 754, 987, 1353
BstMWI GCNNNNNNNGC 3 cut(s) 411, 1228, 1237
BstNI CCWGG 4 cut(s) 556, 885, 1236, 1317
BstNSI RCATGY 1 cut(s) 586
BstPI GGTNACC 1 cut(s) 690
BstSCI CCNGG 4 cut(s) 554, 883, 1234, 1315
BstSLI GKGCMC 2 cut(s) 476, 1233
BstV1I GCAGC 2 cut(s) 788, 1132
BsuRI GGCC 5 cut(s) 405, 554, 932, 1222, 1240
BtgI CCRYGG 2 cut(s) 421, 549
BtsCI GGATG 3 cut(s) 832, 1111, 1201
BtsI GCAGTG 2 cut(s) 476, 591
BtsIMutI CAGTG 5 cut(s) 476, 591, 618, 687, 897
Cac8I GCNNGC 2 cut(s) 584, 588
CciI TCATGA 1 cut(s) 1356
Cfr13I GGNCC 3 cut(s) 346, 362, 1238
Csp6I GTAC 2 cut(s) 370, 1301
CviAII CATG 7 cut(s) 281, 422, 550, 583, 799, 1332, 1357
CviQI GTAC 2 cut(s) 370, 1301
DdeI CTNAG 5 cut(s) 515, 900, 923, 1110, 1149
DpnI GATC 5 cut(s) 430, 735, 756, 989, 1355
DpnII GATC 5 cut(s) 428, 733, 754, 987, 1353
EaeI YGGCCR 1 cut(s) 552
Eam1104I CTCTTC 2 cut(s) 280, 285
EarI CTCTTC 2 cut(s) 280, 285
EciI GGCGGA 1 cut(s) 101
Eco130I CCWWGG 3 cut(s) 421, 482, 549
Eco47I GGWCC 2 cut(s) 346, 362
Eco91I GGTNACC 1 cut(s) 690
EcoO109I RGGNCCY 2 cut(s) 346, 362
EcoO65I GGTNACC 1 cut(s) 690
EcoRII CCWGG 4 cut(s) 554, 883, 1234, 1315
EcoT14I CCWWGG 3 cut(s) 421, 482, 549
ErhI CCWWGG 3 cut(s) 421, 482, 549
FaeI CATG 7 cut(s) 284, 425, 553, 586, 802, 1335, 1360
FalI AAGNNNNNCTT 2 cut(s) 53, 85
FaqI GGGAC 6 cut(s) 332, 403, 556, 985, 1041, 1052
FatI CATG 7 cut(s) 280, 421, 549, 582, 798, 1331, 1356
FbaI TGATCA 1 cut(s) 733
Fnu4HI GCNGC 2 cut(s) 777, 1146
FokI GGATG 3 cut(s) 819, 1118, 1208
Fsp4HI GCNGC 2 cut(s) 777, 1146
FspBI CTAG 2 cut(s) 587, 975
GluI GCNGC 2 cut(s) 777, 1146
GsaI CCCAGC 1 cut(s) 1050
GsuI CTGGAG 1 cut(s) 1370
HaeIII GGCC 5 cut(s) 405, 554, 932, 1222, 1240
Hin1II CATG 7 cut(s) 284, 425, 553, 586, 802, 1335, 1360
HinfI GANTC 4 cut(s) 545, 569, 892, 1369
Hpy166II GTNNAC 3 cut(s) 157, 616, 1301
Hpy188I TCNGA 3 cut(s) 433, 891, 924
Hpy188III TCNNGA 3 cut(s) 61, 1094, 1357
Hpy8I GTNNAC 3 cut(s) 157, 616, 1301
HpyAV CCTTC 6 cut(s) 64, 395, 584, 1022, 1091, 1276
HpyCH4III ACNGT 2 cut(s) 154, 184
HpyCH4V TGCA 4 cut(s) 414, 582, 832, 1288
HpyF10VI GCNNNNNNNGC 3 cut(s) 411, 1228, 1237
HpyF3I CTNAG 5 cut(s) 515, 900, 923, 1110, 1149
Hsp92II CATG 7 cut(s) 284, 425, 553, 586, 802, 1335, 1360
KflI GGGWCCC 1 cut(s) 346
Ksp22I TGATCA 1 cut(s) 733
Kzo9I GATC 5 cut(s) 428, 733, 754, 987, 1353
Lsp1109I GCAGC 2 cut(s) 788, 1132
LweI GCATC 3 cut(s) 423, 841, 1096
MaeI CTAG 2 cut(s) 587, 975
MaeIII GTNAC 4 cut(s) 323, 542, 690, 1000
MalI GATC 5 cut(s) 430, 735, 756, 989, 1355
MboI GATC 5 cut(s) 428, 733, 754, 987, 1353
MfeI CAATTG 1 cut(s) 563
MhlI GDGCHC 2 cut(s) 476, 1233
MlsI TGGCCA 1 cut(s) 554
MluCI AATT 7 cut(s) 393, 409, 563, 743, 759, 996, 1190
MluNI TGGCCA 1 cut(s) 554
MlyI GAGTC 2 cut(s) 539, 578
Mox20I TGGCCA 1 cut(s) 554
MscI TGGCCA 1 cut(s) 554
MseI TTAA 4 cut(s) 396, 705, 1179, 1266
MslI CAYNNNNRTG 1 cut(s) 803
Msp20I TGGCCA 1 cut(s) 554
MspA1I CMGCKG 2 cut(s) 731, 779
MspR9I CCNGG 4 cut(s) 556, 885, 1236, 1317
MunI CAATTG 1 cut(s) 563
MvaI CCWGG 4 cut(s) 556, 885, 1236, 1317
MwoI GCNNNNNNNGC 3 cut(s) 411, 1228, 1237
NcoI CCATGG 2 cut(s) 421, 549
NdeII GATC 5 cut(s) 428, 733, 754, 987, 1353
NheI GCTAGC 1 cut(s) 586
NlaIII CATG 7 cut(s) 284, 425, 553, 586, 802, 1335, 1360
NlaIV GGNNCC 5 cut(s) 347, 348, 363, 560, 1232
NmuCI GTSAC 4 cut(s) 323, 542, 690, 1000
NspI RCATGY 1 cut(s) 586
PaeI GCATGC 1 cut(s) 586
PagI TCATGA 1 cut(s) 1356
PasI CCCWGGG 1 cut(s) 1235
PfeI GAWTC 2 cut(s) 892, 1369
PkrI GCNGC 2 cut(s) 778, 1147
PleI GAGTC 2 cut(s) 539, 577
PpsI GAGTC 2 cut(s) 539, 577
PpuMI RGGWCCY 2 cut(s) 346, 362
Psp5II RGGWCCY 2 cut(s) 346, 362
Psp6I CCWGG 4 cut(s) 554, 883, 1234, 1315
PspEI GGTNACC 1 cut(s) 690
PspFI CCCAGC 1 cut(s) 1046
PspGI CCWGG 4 cut(s) 554, 883, 1234, 1315
PspN4I GGNNCC 5 cut(s) 347, 348, 363, 560, 1232
PspPI GGNCC 3 cut(s) 346, 362, 1238
PspPPI RGGWCCY 2 cut(s) 346, 362
PvuII CAGCTG 2 cut(s) 731, 779
RsaI GTAC 2 cut(s) 371, 1302
RsaNI GTAC 2 cut(s) 370, 1301
RseI CAYNNNNRTG 1 cut(s) 803
SaqAI TTAA 4 cut(s) 396, 705, 1179, 1266
SatI GCNGC 2 cut(s) 777, 1146
Sau3AI GATC 5 cut(s) 428, 733, 754, 987, 1353
Sau96I GGNCC 3 cut(s) 346, 362, 1238
SchI GAGTC 2 cut(s) 539, 578
ScrFI CCNGG 4 cut(s) 556, 885, 1236, 1317
SduI GDGCHC 2 cut(s) 476, 1233
SfaNI GCATC 3 cut(s) 423, 841, 1096
SinI GGWCC 2 cut(s) 346, 362
SmiMI CAYNNNNRTG 1 cut(s) 803
SpeI ACTAGT 1 cut(s) 974
SphI GCATGC 1 cut(s) 586
Sse9I AATT 7 cut(s) 393, 409, 563, 743, 759, 996, 1190
SsiI CCGC 1 cut(s) 112
SspMI CTAG 2 cut(s) 587, 975
StyD4I CCNGG 4 cut(s) 554, 883, 1234, 1315
StyI CCWWGG 3 cut(s) 421, 482, 549
TaaI ACNGT 2 cut(s) 154, 184
TasI AATT 7 cut(s) 393, 409, 563, 743, 759, 996, 1190
TatI WGTACW 2 cut(s) 369, 1300
TfiI GAWTC 2 cut(s) 892, 1369
Tru1I TTAA 4 cut(s) 396, 705, 1179, 1266
Tru9I TTAA 4 cut(s) 396, 705, 1179, 1266
TscAI CASTG 5 cut(s) 476, 598, 625, 687, 904
TseFI GTSAC 4 cut(s) 323, 542, 690, 1000
TseI GCWGC 2 cut(s) 776, 1145
Tsp45I GTSAC 4 cut(s) 323, 542, 690, 1000
TspDTI ATGAA 7 cut(s) 46, 70, 176, 297, 756, 1262, 1373
TspRI CASTG 5 cut(s) 476, 598, 625, 687, 904
VpaK11BI GGWCC 2 cut(s) 346, 362
XceI RCATGY 1 cut(s) 586
XcmI CCANNNNNNNNNTGG 1 cut(s) 562
XspI CTAG 2 cut(s) 587, 975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.