Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008191

Physical Location & Seq

Genomic Coordinates Forward (+)

6493881 .. 6497194

Gene Structure 3314 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1093800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2256 bp

ATGTCGGAATCAAATGAGCATGAATCTCACCCCCCTGACCCACATTCACCACCTCATCATGACTCACCAATCGCGGCAACGTTAAGTGACAGCAAGATCACCGCGCAGCCCCACGATAGTACCGAGGACAGCAAATCAACACCGTCACCGCCTTCCCCACCTCCGCCATCACAAGCGCCCCCTCCGAAATCAGAGTCTCCGCCTCCAAAATCAGACTCACCTGCCCCAAAGTCTTCACCATCCCCCAAACCTTCACCACGACCGCCATCTCCACCGCCATCTCCACCGCCATCATCACCTCCACCATCGTCACCACCTCCGTCTTCTCCATCCCCCTCTTCACCACCACCATCATCATCGCCACCAAAGTCACCTCCTCGTCCTCGCTCACCACCACCTTCACCCTCAAAAGACAAAGGATCGCCATCTGGTTCATCATCGCCTTCTCCTCCAGCTTCTCCACCGCCACCTAGTGGTTCTTCCAATGAGAATGCTCCTCAACCATCATCCTCTGGAGGCAGTTCATCATCTAAATCACCACCAACTGATTCATCACAAGGCGGAGGCTCCTCTCAATCCTCACCTAGTGACTCTCAGTCGCCTCCAGCCTCTCCACCTCCAGCTACACCTTCAACCAACCCCACCACCCCATCTCCTCCTACAGTACCTAGCCCTCCAGCGGTTCCAGCAGGTTCAGGAACTACAACTCAGCCTCTCCCTCGCCCTGCTAATTCCACCCCCACCCTCTCCCCTCCCTCAACCAGAAGTCAGTCTGCAGCTTCAGACGACTCAGGGGGTAGTAGTAGTAGGCATACAGGCACTGTCATAGGCTTGACGTTAGCTGGAGTTTTCCTCATTGCCTTGGTAGCCCTCATTGTTGTGTTTGTAAGGAAGAGAAAGAAGCAGGCACAACCATACCCCCCAAATTACATGCCTCCCACCCCCGGTCCCCCTGGCACTAACTTATCAACTCCGGGTGCATATTATCATGCGCAGCAACAGAGTTCTGCAGCTTCTGTTCCAACAGAAAGCTTCTATTCGAGTGGACCGCACGGACCTTCCCCGCAGAGTTCTGGAGATTCCGGTCTCATTGCTGCTAACAAGGTTCATTTCACCTACGAAGAATTGATGGAGATAACGAATGGATTTTCACGTCAAAACGTTCTCGGTGAGGGTGGTTTCGGATGCGTGTACAAGGGTTGGATGCCGGACGGAAGAGTTGTGGCAGTCAAGCAGCTCAAGGCTGGTAGTGGGCAGGGAGAGAAGGAATTTAGGGCTGAAGTTGAGATTATTAGTCGCGTGCATCATCGTTACTTGCTCTCTCTGGTGGGATACTGCATCTCTGAACAGCAAAGATTGCTTATCTATGAATTTGTTCCCAATAAAACTCTTGAGCACCATTTGCATGGTGCCGGAATGCCGGTGTTGGAATGGACCAAGAGACTCAAGGTTGCCTTGGGGTCTGCAAAAGGCTTGGCGTATTTACACGAAGACTGCCATCCAAAAATCATTCACAGAGACATTAAATCGGCAAACATTCTGCTGGATGATGCCTTTGAAGCACAGGTTGCAGACTTTGGGCTTGCCAAACTTACAAACGACATGAATACTCACGTATCCACTCGGGTCATGGGAACATTCGGGTACATGGCACCTGAGTATGCAAATAGTGGCAAATTGACGGACAGATCAGATGTATATTCGTTCGGAGTTGTGCTTCTAGAGCTTGTAACTGGGCGGAAACCTGTTGATCCAACTCGACCTTTAGGGGATGAGAGTTTGGTTGAATGGGCTCGTCCACTTCTTATTCAAGCCCTTGAAACTGGGGATTTGAGTGAATTAGTAGATCCTCGGCTTGAGAAACATTATGTGGAGGGTGAAATGTTCAGAATGATCGAGGCTGCTGCTGCTTGCGTGCGTCATTCTGCTCTAAAACGACCACGAATGGCGCAGGTGGTAAGAGCATTGGACTGTGAAGGCGAATTATCTGATCTAACGAATGGAGTGAAAGTTGGGCAGAGTACTGCATATGACTCTGGCCAGTACAATGAAGACATCAATAGATTCAGAAGGATGGCATTAGGAAGCGGAGGCAGCTCAGAGATATCCAGTGCAGATTTCACTTCCAGAGAGGTATCAGGACCCCAAAGTTTATGGACTCGCCAGGATCCTTCAAGTGATGAATCAGATACTCGAGCTTTTACCACGCGTGGTGGTGAGCAGATAATCAGCGGAAATCAAGGCAGACGGTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0032502 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

752

Amino Acids

79.09

Weight (kDa)

6.51

Isoelectric Point (pI)

82.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	384 - 584	1.9e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	386 - 656	8.7e-45	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008191)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10620 AT1G23540 AT1G23540 AT1G70450 AT1G70460
fragaria_vesca	FvH4_4g34330
malus_domestica	MD13G1092900.v1.1 MD16G1093800.v1.1
prunus_persica	Prupe.1G252700_v2.0.a1 Prupe.1G252700_v2.0.a1 Prupe.1G252700_v2.0.a1
pyrus_communis	pycom13g08090
rosa_chinensis	RchiOBHm_Chr4g0443751
rosa_laevigata	RLG00000005893
rosa_multiflora	Rmu_co8490185.1_g000001 Rmu_sc0001426.1_g000001
rosa_roxburghii	Rroxscaffold_5G00384320
rosa_rugosa	Rorug04G0347100
rosa_samantha	Rh4AG407200 Rh4BG418100 Rh4CG432300 Rh4DG413400
rosa_wichuraiana	Rw4G034970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	229, 1942
Acc16I	TGCGCA	1 cut(s)	993
Acc36I	ACCTGC	3 cut(s)	229, 680, 1942
AccB1I	GGYRCC	2 cut(s)	1409, 1651
AccB7I	CCANNNNNTGG	1 cut(s)	473
AccII	CGCG	4 cut(s)	74, 104, 1299, 2209
AclI	AACGTT	2 cut(s)	80, 1161
AclWI	GGATC	5 cut(s)	427, 1745, 1841, 2162, 2175
AcoI	YGGCCR	1 cut(s)	2038
AcsI	RAATTY	2 cut(s)	1268, 1370
AcuI	CTGAAG	2 cut(s)	765, 1299
AdeI	CACNNNGTG	2 cut(s)	473, 587
AfaI	GTAC	6 cut(s)	121, 666, 1193, 1646, 2023, 2045
AfiI	CCNNNNNNNGG	3 cut(s)	473, 679, 944
AflIII	ACRYGT	1 cut(s)	2207
AgsI	TTSAA	6 cut(s)	633, 1559, 1787, 1811, 1820, 2175
AhdI	GACNNNNNGTC	1 cut(s)	595
AjiI	CACGTC	1 cut(s)	1154
AjnI	CCWGG	2 cut(s)	952, 2163
Alw21I	GWGCWC	1 cut(s)	1398
Alw26I	GTCTC	4 cut(s)	201, 1091, 1435, 1512
AlwI	GGATC	5 cut(s)	427, 1745, 1841, 2162, 2175
AlwNI	CAGNNNCTG	2 cut(s)	821, 1016
Ama87I	CYCGRG	2 cut(s)	1623, 2193
AoxI	GGCC	1 cut(s)	2038
ApoI	RAATTY	2 cut(s)	1268, 1370
ArsI	GACNNNNNNTTYG	2 cut(s)	179, 211
Asp700I	GAANNNNTTC	1 cut(s)	1374
AspLEI	GCGC	4 cut(s)	106, 178, 994, 1951
AspS9I	GGNCC	5 cut(s)	947, 1046, 1055, 1434, 2141
AsuC2I	CCSGG	2 cut(s)	945, 975
AvaI	CYCGRG	2 cut(s)	1623, 2193
AvaII	GGWCC	5 cut(s)	947, 1046, 1055, 1434, 2141
BaeI	ACNNNNGTAYC	4 cut(s)	103, 136, 1599, 1632
BalI	TGGCCA	1 cut(s)	2040
BamHI	GGATCC	1 cut(s)	2167
BanI	GGYRCC	2 cut(s)	1409, 1651
BanII	GRGCYC	1 cut(s)	1795
BbsI	GAAGAC	4 cut(s)	225, 315, 1497, 2058
Bbv12I	GWGCWC	1 cut(s)	1398
BcgI	CGANNNNNNTGC	2 cut(s)	1886, 1920
BciT130I	CCWGG	2 cut(s)	954, 2165
BciVI	GTATCC	2 cut(s)	1325, 1627
BcnI	CCSGG	2 cut(s)	945, 975
BcoDI	GTCTC	4 cut(s)	201, 1091, 1435, 1512
BfaI	CTAG	4 cut(s)	471, 585, 669, 1721
BfmI	CTRYAG	3 cut(s)	660, 774, 1008
BfoI	RGCGCY	1 cut(s)	179
BfuAI	ACCTGC	3 cut(s)	229, 680, 1942
BfuI	GTATCC	2 cut(s)	1325, 1627
BmcAI	AGTACT	1 cut(s)	2023
Bme1390I	CCNGG	4 cut(s)	945, 954, 975, 2165
Bme18I	GGWCC	5 cut(s)	947, 1046, 1055, 1434, 2141
BmeRI	GACNNNNNGTC	1 cut(s)	595
BmeT110I	CYCGRG	2 cut(s)	1623, 2193
BmgBI	CACGTC	1 cut(s)	1154
BmgT120I	GGNCC	5 cut(s)	947, 1046, 1055, 1434, 2141
BmiI	GGNNCC	7 cut(s)	568, 684, 949, 1411, 1653, 2143, 2169
BmrFI	CCNGG	4 cut(s)	945, 954, 975, 2165
BmrI	ACTGGG	2 cut(s)	1743, 1833
BmsI	GCATC	5 cut(s)	1175, 1194, 1312, 1347, 1540
BmuI	ACTGGG	2 cut(s)	1743, 1833
BpiI	GAAGAC	4 cut(s)	225, 315, 1497, 2058
BplI	GAGNNNNNCTC	2 cut(s)	837, 869
BpmI	CTGGAG	7 cut(s)	435, 534, 588, 603, 660, 864, 1095
BpuEI	CTTGAG	4 cut(s)	1223, 1412, 1430, 1877
BpuMI	CCSGG	2 cut(s)	945, 975
BsaAI	YACGTR	1 cut(s)	1615
BsaBI	GATNNNNATC	1 cut(s)	424
BsaI	GGTCTC	1 cut(s)	1091
BsaJI	CCNNGG	6 cut(s)	123, 861, 943, 952, 1455, 1850
BsaWI	WCCGGW	1 cut(s)	1082
BsaXI	ACNNNNNCTCC	4 cut(s)	637, 667, 1995, 2025
Bsc4I	CCNNNNNNNGG	3 cut(s)	473, 679, 944
Bse118I	RCCGGY	1 cut(s)	1420
Bse1I	ACTGG	4 cut(s)	1738, 1828, 2041, 2109
Bse3DI	GCAATG	2 cut(s)	855, 1089
Bse8I	GATNNNNATC	1 cut(s)	424
BseBI	CCWGG	2 cut(s)	954, 2165
BseDI	CCNNGG	6 cut(s)	123, 861, 943, 952, 1455, 1850
BseGI	GGATG	9 cut(s)	239, 329, 506, 1190, 1209, 1498, 1552, 1777, 2079
BseJI	GATNNNNATC	1 cut(s)	424
BseLI	CCNNNNNNNGG	3 cut(s)	473, 679, 944
BseMI	GCAATG	2 cut(s)	855, 1089
BseMII	CTCAG	5 cut(s)	608, 722, 804, 1647, 2112
BseNI	ACTGG	4 cut(s)	1738, 1828, 2041, 2109
BseRI	GAGGAG	5 cut(s)	366, 438, 486, 559, 645
BsgI	GTGCAG	1 cut(s)	2133
Bsh1236I	CGCG	4 cut(s)	74, 104, 1299, 2209
Bsh1285I	CGRYCG	1 cut(s)	263
BshFI	GGCC	1 cut(s)	2040
BshNI	GGYRCC	2 cut(s)	1409, 1651
BsiEI	CGRYCG	1 cut(s)	263
BsiHKAI	GWGCWC	1 cut(s)	1398
BsiHKCI	CYCGRG	2 cut(s)	1623, 2193
BsiSI	CCGG	6 cut(s)	945, 974, 1083, 1208, 1413, 1421
BslFI	GGGAC	1 cut(s)	933
BslI	CCNNNNNNNGG	3 cut(s)	473, 679, 944
BsmAI	GTCTC	4 cut(s)	201, 1091, 1435, 1512
BsmFI	GGGAC	1 cut(s)	933
BsmI	GAATGC	2 cut(s)	496, 1422
BsnI	GGCC	1 cut(s)	2040
Bso31I	GGTCTC	1 cut(s)	1091
BsoBI	CYCGRG	2 cut(s)	1623, 2193
Bsp1286I	GDGCHC	2 cut(s)	1398, 1795
Bsp1407I	TGTACA	1 cut(s)	1191
Bsp143I	GATC	8 cut(s)	96, 419, 1688, 1750, 1846, 1893, 1990, 2167
BspANI	GGCC	1 cut(s)	2040
BspCNI	CTCAG	5 cut(s)	607, 721, 803, 1648, 2111
BspFNI	CGCG	4 cut(s)	74, 104, 1299, 2209
BspHI	TCATGA	1 cut(s)	58
BspLI	GGNNCC	7 cut(s)	568, 684, 949, 1411, 1653, 2143, 2169
BspMAI	CTGCAG	2 cut(s)	778, 1012
BspMI	ACCTGC	3 cut(s)	229, 680, 1942
BspPI	GGATC	5 cut(s)	427, 1745, 1841, 2162, 2175
BspT107I	GGYRCC	2 cut(s)	1409, 1651
BspTNI	GGTCTC	1 cut(s)	1091
BsrDI	GCAATG	2 cut(s)	855, 1089
BsrFI	RCCGGY	1 cut(s)	1420
BsrGI	TGTACA	1 cut(s)	1191
BsrI	ACTGG	4 cut(s)	1738, 1828, 2041, 2109
BssAI	RCCGGY	1 cut(s)	1420
BssECI	CCNNGG	6 cut(s)	123, 861, 943, 952, 1455, 1850
BssMI	GATC	8 cut(s)	96, 419, 1688, 1750, 1846, 1893, 1990, 2167
BssT1I	CCWWGG	2 cut(s)	861, 1455
Bst2UI	CCWGG	2 cut(s)	954, 2165
Bst4CI	ACNGT	5 cut(s)	144, 664, 823, 1973, 2250
Bst6I	CTCTTC	3 cut(s)	343, 887, 1210
BstAPI	GCANNNNNTGC	3 cut(s)	1357, 1402, 1568
BstAUI	TGTACA	1 cut(s)	1191
BstBAI	YACGTR	1 cut(s)	1615
BstC8I	GCNNGC	5 cut(s)	906, 1301, 1584, 1912, 1916
BstDEI	CTNAG	5 cut(s)	594, 708, 790, 1656, 2098
BstF5I	GGATG	9 cut(s)	239, 329, 506, 1190, 1209, 1498, 1552, 1777, 2079
BstFNI	CGCG	4 cut(s)	74, 104, 1299, 2209
BstH2I	RGCGCY	1 cut(s)	179
BstHHI	GCGC	4 cut(s)	106, 178, 994, 1951
BstKTI	GATC	8 cut(s)	99, 422, 1691, 1753, 1849, 1896, 1993, 2170
BstMAI	GTCTC	4 cut(s)	201, 1091, 1435, 1512
BstMBI	GATC	8 cut(s)	96, 419, 1688, 1750, 1846, 1893, 1990, 2167
BstMCI	CGRYCG	1 cut(s)	263
BstMWI	GCNNNNNNNGC	9 cut(s)	686, 866, 1357, 1402, 1559, 1568, 1723, 1907, 2094
BstNI	CCWGG	2 cut(s)	954, 2165
BstNSI	RCATGY	1 cut(s)	934
BstSCI	CCNGG	4 cut(s)	943, 952, 973, 2163
BstSFI	CTRYAG	3 cut(s)	660, 774, 1008
BstUI	CGCG	4 cut(s)	74, 104, 1299, 2209
BstV2I	GAAGAC	4 cut(s)	225, 315, 1497, 2058
BstX2I	RGATCY	2 cut(s)	1846, 2167
BstXI	CCANNNNNNTGG	1 cut(s)	1406
BstYI	RGATCY	2 cut(s)	1846, 2167
BsuI	GTATCC	2 cut(s)	1325, 1627
BsuRI	GGCC	1 cut(s)	2040
BtgZI	GCGATG	2 cut(s)	342, 423
BtrI	CACGTC	1 cut(s)	1154
BtsCI	GGATG	9 cut(s)	239, 329, 506, 1190, 1209, 1498, 1552, 1777, 2079
BtsIMutI	CAGTG	2 cut(s)	819, 2116
BveI	ACCTGC	3 cut(s)	229, 680, 1942
Cac8I	GCNNGC	5 cut(s)	906, 1301, 1584, 1912, 1916
CaiI	CAGNNNCTG	2 cut(s)	821, 1016
CciI	TCATGA	1 cut(s)	58
CfoI	GCGC	4 cut(s)	106, 178, 994, 1951
Cfr10I	RCCGGY	1 cut(s)	1420
Cfr13I	GGNCC	5 cut(s)	947, 1046, 1055, 1434, 2141
CseI	GACGC	1 cut(s)	1907
Csp6I	GTAC	6 cut(s)	120, 665, 1192, 1645, 2022, 2044
CviAII	CATG	8 cut(s)	20, 59, 931, 989, 1406, 1603, 1630, 1648
CviQI	GTAC	6 cut(s)	120, 665, 1192, 1645, 2022, 2044
DdeI	CTNAG	5 cut(s)	594, 708, 790, 1656, 2098
DpnI	GATC	8 cut(s)	98, 421, 1690, 1752, 1848, 1895, 1992, 2169
DpnII	GATC	8 cut(s)	96, 419, 1688, 1750, 1846, 1893, 1990, 2167
DraIII	CACNNNGTG	2 cut(s)	473, 587
DriI	GACNNNNNGTC	1 cut(s)	595
EaeI	YGGCCR	1 cut(s)	2038
Eam1104I	CTCTTC	3 cut(s)	343, 887, 1210
Eam1105I	GACNNNNNGTC	1 cut(s)	595
EarI	CTCTTC	3 cut(s)	343, 887, 1210
EciI	GGCGGA	4 cut(s)	153, 189, 576, 1753
Eco130I	CCWWGG	2 cut(s)	861, 1455
Eco24I	GRGCYC	1 cut(s)	1795
Eco31I	GGTCTC	1 cut(s)	1091
Eco32I	GATATC	1 cut(s)	2106
Eco47I	GGWCC	5 cut(s)	947, 1046, 1055, 1434, 2141
Eco57I	CTGAAG	2 cut(s)	765, 1299
Eco88I	CYCGRG	2 cut(s)	1623, 2193
EcoO109I	RGGNCCY	1 cut(s)	2141
EcoRII	CCWGG	2 cut(s)	952, 2163
EcoRV	GATATC	1 cut(s)	2106
EcoT14I	CCWWGG	2 cut(s)	861, 1455
EcoT38I	GRGCYC	1 cut(s)	1795
ErhI	CCWWGG	2 cut(s)	861, 1455
FaeI	CATG	8 cut(s)	23, 62, 934, 992, 1409, 1606, 1633, 1651
FalI	AAGNNNNNCTT	2 cut(s)	1439, 1471
FaqI	GGGAC	1 cut(s)	933
FatI	CATG	8 cut(s)	19, 58, 930, 988, 1405, 1602, 1629, 1647
FauI	CCCGC	1 cut(s)	1071
FauNDI	CATATG	1 cut(s)	2029
FokI	GGATG	9 cut(s)	226, 316, 493, 1197, 1216, 1485, 1559, 1784, 2086
FriOI	GRGCYC	1 cut(s)	1795
FspBI	CTAG	4 cut(s)	471, 585, 669, 1721
FspI	TGCGCA	1 cut(s)	993
GlaI	GCGC	4 cut(s)	105, 177, 993, 1950
GsuI	CTGGAG	7 cut(s)	435, 534, 588, 603, 660, 864, 1095
HaeII	RGCGCY	1 cut(s)	179
HaeIII	GGCC	1 cut(s)	2040
HapII	CCGG	6 cut(s)	945, 974, 1083, 1208, 1413, 1421
HgaI	GACGC	1 cut(s)	1907
HhaI	GCGC	4 cut(s)	106, 178, 994, 1951
Hin1II	CATG	8 cut(s)	23, 62, 934, 992, 1409, 1606, 1633, 1651
Hin6I	GCGC	4 cut(s)	104, 176, 992, 1949
HinP1I	GCGC	4 cut(s)	104, 176, 992, 1949
HindIII	AAGCTT	1 cut(s)	1030
HpaII	CCGG	6 cut(s)	945, 974, 1083, 1208, 1413, 1421
Hpy166II	GTNNAC	3 cut(s)	1046, 1192, 1799
Hpy188III	TCNNGA	8 cut(s)	59, 513, 696, 1074, 1391, 1721, 2127, 2139
Hpy8I	GTNNAC	3 cut(s)	1046, 1192, 1799
HpyCH4III	ACNGT	5 cut(s)	144, 664, 823, 1973, 2250
HpyCH4IV	ACGT	5 cut(s)	80, 836, 1153, 1161, 1614
HpyF10VI	GCNNNNNNNGC	9 cut(s)	686, 866, 1357, 1402, 1559, 1568, 1723, 1907, 2094
HpyF3I	CTNAG	5 cut(s)	594, 708, 790, 1656, 2098
HpySE526I	ACGT	5 cut(s)	80, 836, 1153, 1161, 1614
Hsp92II	CATG	8 cut(s)	23, 62, 934, 992, 1409, 1606, 1633, 1651
HspAI	GCGC	4 cut(s)	104, 176, 992, 1949
Kzo9I	GATC	8 cut(s)	96, 419, 1688, 1750, 1846, 1893, 1990, 2167
LmnI	GCTCC	2 cut(s)	499, 572
LweI	GCATC	5 cut(s)	1175, 1194, 1312, 1347, 1540
MaeI	CTAG	4 cut(s)	471, 585, 669, 1721
MaeII	ACGT	5 cut(s)	80, 836, 1153, 1161, 1614
MaeIII	GTNAC	7 cut(s)	86, 144, 309, 369, 587, 1310, 1729
MalI	GATC	8 cut(s)	98, 421, 1690, 1752, 1848, 1895, 1992, 2169
MboI	GATC	8 cut(s)	96, 419, 1688, 1750, 1846, 1893, 1990, 2167
MboII	GAAGA	9 cut(s)	225, 315, 330, 471, 904, 1133, 1227, 1502, 2063
MflI	RGATCY	2 cut(s)	1846, 2167
MhlI	GDGCHC	2 cut(s)	1398, 1795
MlsI	TGGCCA	1 cut(s)	2040
MluCI	AATT	8 cut(s)	730, 925, 1124, 1268, 1370, 1676, 1838, 1982
MluI	ACGCGT	1 cut(s)	2207
MluNI	TGGCCA	1 cut(s)	2040
MlyI	GAGTC	8 cut(s)	56, 203, 209, 584, 782, 1437, 2027, 2152
MmeI	TCCRAC	4 cut(s)	1046, 1181, 1407, 1778
Mox20I	TGGCCA	1 cut(s)	2040
MroXI	GAANNNNTTC	1 cut(s)	1374
MscI	TGGCCA	1 cut(s)	2040
MseI	TTAA	2 cut(s)	83, 1524
MslI	CAYNNNNRTG	2 cut(s)	878, 1404
Msp20I	TGGCCA	1 cut(s)	2040
MspA1I	CMGCKG	2 cut(s)	680, 2232
MspI	CCGG	6 cut(s)	945, 974, 1083, 1208, 1413, 1421
MspR9I	CCNGG	4 cut(s)	945, 954, 975, 2165
Mva1269I	GAATGC	2 cut(s)	496, 1422
MvaI	CCWGG	2 cut(s)	954, 2165
MvnI	CGCG	4 cut(s)	74, 104, 1299, 2209
MwoI	GCNNNNNNNGC	9 cut(s)	686, 866, 1357, 1402, 1559, 1568, 1723, 1907, 2094
NciI	CCSGG	2 cut(s)	945, 975
NdeI	CATATG	1 cut(s)	2029
NdeII	GATC	8 cut(s)	96, 419, 1688, 1750, 1846, 1893, 1990, 2167
NlaIII	CATG	8 cut(s)	23, 62, 934, 992, 1409, 1606, 1633, 1651
NlaIV	GGNNCC	7 cut(s)	568, 684, 949, 1411, 1653, 2143, 2169
NmeAIII	GCCGAG	1 cut(s)	1831
NmuCI	GTSAC	5 cut(s)	86, 144, 309, 369, 587
NsbI	TGCGCA	1 cut(s)	993
NspI	RCATGY	1 cut(s)	934
PaeR7I	CTCGAG	1 cut(s)	2193
PagI	TCATGA	1 cut(s)	58
PaqCI	CACCTGC	2 cut(s)	229, 1942
PcsI	WCGNNNNNNNCGW	1 cut(s)	120
PctI	GAATGC	2 cut(s)	496, 1422
PdmI	GAANNNNTTC	1 cut(s)	1374
PfeI	GAWTC	6 cut(s)	8, 23, 548, 1079, 2064, 2183
PflMI	CCANNNNNTGG	1 cut(s)	473
PleI	GAGTC	8 cut(s)	56, 202, 209, 584, 782, 1437, 2027, 2152
PpsI	GAGTC	8 cut(s)	56, 202, 209, 584, 782, 1437, 2027, 2152
Ppu21I	YACGTR	1 cut(s)	1615
PpuMI	RGGWCCY	1 cut(s)	2141
Psp1406I	AACGTT	2 cut(s)	80, 1161
Psp5II	RGGWCCY	1 cut(s)	2141
Psp6I	CCWGG	2 cut(s)	952, 2163
PspGI	CCWGG	2 cut(s)	952, 2163
PspN4I	GGNNCC	7 cut(s)	568, 684, 949, 1411, 1653, 2143, 2169
PspPI	GGNCC	5 cut(s)	947, 1046, 1055, 1434, 2141
PspPPI	RGGWCCY	1 cut(s)	2141
PspXI	VCTCGAGB	1 cut(s)	2193
PstI	CTGCAG	2 cut(s)	778, 1012
PstNI	CAGNNNCTG	2 cut(s)	821, 1016
PsuI	RGATCY	2 cut(s)	1846, 2167
RsaI	GTAC	6 cut(s)	121, 666, 1193, 1646, 2023, 2045
RsaNI	GTAC	6 cut(s)	120, 665, 1192, 1645, 2022, 2044
RseI	CAYNNNNRTG	2 cut(s)	878, 1404
SaqAI	TTAA	2 cut(s)	83, 1524
Sau3AI	GATC	8 cut(s)	96, 419, 1688, 1750, 1846, 1893, 1990, 2167
Sau96I	GGNCC	5 cut(s)	947, 1046, 1055, 1434, 2141
ScaI	AGTACT	1 cut(s)	2023
SchI	GAGTC	8 cut(s)	56, 203, 209, 584, 782, 1437, 2027, 2152
ScrFI	CCNGG	4 cut(s)	945, 954, 975, 2165
SduI	GDGCHC	2 cut(s)	1398, 1795
SfaNI	GCATC	5 cut(s)	1175, 1194, 1312, 1347, 1540
SfcI	CTRYAG	3 cut(s)	660, 774, 1008
Sfr274I	CTCGAG	1 cut(s)	2193
SinI	GGWCC	5 cut(s)	947, 1046, 1055, 1434, 2141
SlaI	CTCGAG	1 cut(s)	2193
SmiMI	CAYNNNNRTG	2 cut(s)	878, 1404
SmlI	CTYRAG	5 cut(s)	1238, 1391, 1445, 1856, 2193
SmoI	CTYRAG	5 cut(s)	1238, 1391, 1445, 1856, 2193
Sse9I	AATT	8 cut(s)	730, 925, 1124, 1268, 1370, 1676, 1838, 1982
SspMI	CTAG	4 cut(s)	471, 585, 669, 1721
StyD4I	CCNGG	4 cut(s)	943, 952, 973, 2163
StyI	CCWWGG	2 cut(s)	861, 1455
TaaI	ACNGT	5 cut(s)	144, 664, 823, 1973, 2250
TaiI	ACGT	5 cut(s)	83, 839, 1156, 1164, 1617
TaqI	TCGA	4 cut(s)	1040, 1759, 1896, 2194
TasI	AATT	8 cut(s)	730, 925, 1124, 1268, 1370, 1676, 1838, 1982
TatI	WGTACW	3 cut(s)	1191, 2021, 2043
TauI	GCSGC	1 cut(s)	77
TfiI	GAWTC	6 cut(s)	8, 23, 548, 1079, 2064, 2183
Tru1I	TTAA	2 cut(s)	83, 1524
Tru9I	TTAA	2 cut(s)	83, 1524
TscAI	CASTG	2 cut(s)	826, 2116
TseFI	GTSAC	5 cut(s)	86, 144, 309, 369, 587
Tsp45I	GTSAC	5 cut(s)	86, 144, 309, 369, 587
TspDTI	ATGAA	9 cut(s)	36, 423, 513, 540, 1097, 1383, 1619, 2064, 2196
TspGWI	ACGGA	4 cut(s)	309, 1068, 1227, 1697
TspRI	CASTG	2 cut(s)	826, 2116
Van91I	CCANNNNNTGG	1 cut(s)	473
VpaK11BI	GGWCC	5 cut(s)	947, 1046, 1055, 1434, 2141
XapI	RAATTY	2 cut(s)	1268, 1370
XbaI	TCTAGA	1 cut(s)	1720
XceI	RCATGY	1 cut(s)	934
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1627
XhoI	CTCGAG	1 cut(s)	2193
XmnI	GAANNNNTTC	1 cut(s)	1374
XspI	CTAG	4 cut(s)	471, 585, 669, 1721
ZrmI	AGTACT	1 cut(s)	2023

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.