MD16G1117200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016830
Physical Location & Seq
Forward (+)
8315717 .. 8317027
1311 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1117200.v1.1.491

Sequence Viewer

Length: 675 bp
ATGGTCGGACCCAAGGACTGCAAAAGCCAATGCTTAGCTTTCAAGACCAATAAACTTGTTCACCAGATTATAGTTGCGCCAAACAAACCTTGTGTCACTTCCTTAATGTACAAAACCAACCATGTAACAAACCCTCCACATCTTCTTGAAGAGATGGGAAATTGTGTTCCAGTTCATAAGACCCAAGACTCTGCTTCAAATCTCAAATGTTCAAGCAATTCCCTGACAGAATGCATCCTGATTGAACTCCCTGTTCGAGAAAACTCCCTTGGTACCGCCATCCCCCGCGCGAGCAGTTTTCAAGAACCAAGTGGGAAAGAGTTTGAATCTGATCGTGAAGATTTCTTCAGTGTCAATGGTGATCTTACCCCATCTGCAAGCAACACTCCGATCCATGAAATAAAAACTGATGCGAAAAAGCAGCTGATTGAATTGTTCCATGAGAGCGACGGTGATGGATCAGAAGACGAACCTTCCATTTTCGACCTGCCACAAAATTCAGCAAATAAAAGTTTATCGTATGAGTCTGTAGCGGACATAGTTTTCAGAAGTGTGACAGTTCCGAAAACGAATTCGAATCCCAGCAGGACAAGAAAAGCTACACTGTTTGCACAATATTGCTGTCTGCCTAAACTGGTGCGGAGCCTAAGCTGTAGTGAGAGGAAGAGTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

225

Amino Acids

24.73

Weight (kDa)

6.59

Isoelectric Point (pI)

47.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016830)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23980
malus_domestica MD16G1117200.v1.1
prunus_persica Prupe.1G229000_v2.0.a1
pyrus_communis pycom16g10040
rosa_chinensis RchiOBHm_Chr4g0430641
rosa_laevigata RLG00000006960
rosa_multiflora Rmu_sc0000131.1_g000014
rosa_roxburghii Rroxscaffold_5G00372390
rosa_rugosa Rorug04G0245000
rosa_samantha Rh4AG301000 Rh4BG308100 Rh4CG325200 Rh4DG305700
rosa_wichuraiana Rw4G026120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 495
Acc65I GGTACC 1 cut(s) 272
AccB1I GGYRCC 1 cut(s) 272
AccII CGCG 2 cut(s) 288, 290
AciI CCGC 4 cut(s) 276, 286, 533, 640
AclWI GGATC 2 cut(s) 385, 466
AcsI RAATTY 2 cut(s) 496, 571
AcuI CTGAAG 1 cut(s) 331
AfaI GTAC 2 cut(s) 110, 274
AgsI TTSAA 8 cut(s) 43, 149, 198, 213, 245, 302, 326, 431
AjuI GAANNNNNNNTTGG 2 cut(s) 252, 284
AloI GAACNNNNNNTCC 2 cut(s) 150, 182
AluBI AGCT 4 cut(s) 38, 424, 599, 651
AluI AGCT 4 cut(s) 38, 424, 599, 651
AlwI GGATC 2 cut(s) 385, 466
ApeKI GCWGC 1 cut(s) 421
ApoI RAATTY 2 cut(s) 496, 571
ArsI GACNNNNNNTTYG 2 cut(s) 78, 110
Asp718I GGTACC 1 cut(s) 272
AspLEI GCGC 2 cut(s) 79, 290
AspS9I GGNCC 1 cut(s) 8
AsuHPI GGTGA 3 cut(s) 53, 371, 464
AsuII TTCGAA 1 cut(s) 575
AvaII GGWCC 1 cut(s) 8
BanI GGYRCC 1 cut(s) 272
BbsI GAAGAC 1 cut(s) 471
BbvI GCAGC 1 cut(s) 433
BccI CCATC 4 cut(s) 148, 287, 379, 449
BfmI CTRYAG 2 cut(s) 528, 652
BfuAI ACCTGC 1 cut(s) 495
BisI GCNGC 1 cut(s) 422
BlpI GCTNAGC 1 cut(s) 34
BlsI GCNGC 1 cut(s) 423
Bme18I GGWCC 1 cut(s) 8
BmgT120I GGNCC 1 cut(s) 8
BmiI GGNNCC 3 cut(s) 10, 274, 644
BmsI GCATC 2 cut(s) 243, 400
BpiI GAAGAC 1 cut(s) 471
Bpu10I CCTNAGC 1 cut(s) 647
Bpu1102I GCTNAGC 1 cut(s) 34
Bpu14I TTCGAA 1 cut(s) 575
BsaJI CCNNGG 2 cut(s) 12, 268
BsaXI ACNNNNNCTCC 2 cut(s) 118, 148
Bse1I ACTGG 2 cut(s) 170, 639
BseDI CCNNGG 2 cut(s) 12, 268
BseGI GGATG 2 cut(s) 234, 279
BseNI ACTGG 2 cut(s) 170, 639
BseXI GCAGC 1 cut(s) 433
BseYI CCCAGC 1 cut(s) 581
Bsh1236I CGCG 2 cut(s) 288, 290
BshNI GGYRCC 1 cut(s) 272
BsmI GAATGC 1 cut(s) 236
Bsp119I TTCGAA 1 cut(s) 575
Bsp1407I TGTACA 1 cut(s) 108
Bsp143I GATC 4 cut(s) 331, 361, 390, 458
Bsp1720I GCTNAGC 1 cut(s) 34
BspACI CCGC 4 cut(s) 276, 286, 533, 640
BspFNI CGCG 2 cut(s) 288, 290
BspLI GGNNCC 3 cut(s) 10, 274, 644
BspMI ACCTGC 1 cut(s) 495
BspPI GGATC 2 cut(s) 385, 466
BspT104I TTCGAA 1 cut(s) 575
BspT107I GGYRCC 1 cut(s) 272
BsrGI TGTACA 1 cut(s) 108
BsrI ACTGG 2 cut(s) 170, 639
BssECI CCNNGG 2 cut(s) 12, 268
BssMI GATC 4 cut(s) 331, 361, 390, 458
BssT1I CCWWGG 2 cut(s) 12, 268
Bst4CI ACNGT 3 cut(s) 452, 559, 606
Bst6I CTCTTC 2 cut(s) 144, 659
BstAUI TGTACA 1 cut(s) 108
BstBI TTCGAA 1 cut(s) 575
BstC8I GCNNGC 2 cut(s) 292, 379
BstDEI CTNAG 2 cut(s) 34, 647
BstF5I GGATG 2 cut(s) 234, 279
BstFNI CGCG 2 cut(s) 288, 290
BstHHI GCGC 2 cut(s) 79, 290
BstKTI GATC 4 cut(s) 334, 364, 393, 461
BstMBI GATC 4 cut(s) 331, 361, 390, 458
BstSFI CTRYAG 2 cut(s) 528, 652
BstUI CGCG 2 cut(s) 288, 290
BstV1I GCAGC 1 cut(s) 433
BstV2I GAAGAC 1 cut(s) 471
BtsCI GGATG 2 cut(s) 234, 279
BtsIMutI CAGTG 2 cut(s) 355, 602
BveI ACCTGC 1 cut(s) 495
Cac8I GCNNGC 2 cut(s) 292, 379
CfoI GCGC 2 cut(s) 79, 290
Cfr13I GGNCC 1 cut(s) 8
Csp6I GTAC 2 cut(s) 109, 273
CviAII CATG 3 cut(s) 122, 395, 440
CviJI RGCY 6 cut(s) 27, 38, 424, 599, 645, 651
CviKI_1 RGCY 6 cut(s) 27, 38, 424, 599, 645, 651
CviQI GTAC 2 cut(s) 109, 273
DdeI CTNAG 2 cut(s) 34, 647
DpnI GATC 4 cut(s) 333, 363, 392, 460
DpnII GATC 4 cut(s) 331, 361, 390, 458
Eam1104I CTCTTC 2 cut(s) 144, 659
EarI CTCTTC 2 cut(s) 144, 659
Eco130I CCWWGG 2 cut(s) 12, 268
Eco47I GGWCC 1 cut(s) 8
Eco57I CTGAAG 1 cut(s) 331
EcoRI GAATTC 1 cut(s) 571
EcoT14I CCWWGG 2 cut(s) 12, 268
EcoT22I ATGCAT 1 cut(s) 236
ErhI CCWWGG 2 cut(s) 12, 268
FaeI CATG 3 cut(s) 125, 398, 443
FaiI YATR 7 cut(s) 71, 123, 177, 396, 441, 522, 539
FatI CATG 3 cut(s) 121, 394, 439
FauI CCCGC 1 cut(s) 293
Fnu4HI GCNGC 1 cut(s) 422
FokI GGATG 2 cut(s) 221, 266
Fsp4HI GCNGC 1 cut(s) 422
GlaI GCGC 2 cut(s) 78, 289
GluI GCNGC 1 cut(s) 422
GsaI CCCAGC 1 cut(s) 585
HhaI GCGC 2 cut(s) 79, 290
Hin1II CATG 3 cut(s) 125, 398, 443
Hin6I GCGC 2 cut(s) 77, 288
HinP1I GCGC 2 cut(s) 77, 288
HinfI GANTC 5 cut(s) 188, 326, 524, 577, 667
HphI GGTGA 3 cut(s) 53, 371, 464
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 6 cut(s) 8, 331, 390, 463, 548, 564
Hpy188III TCNNGA 6 cut(s) 43, 146, 238, 257, 302, 335
Hpy8I GTNNAC 1 cut(s) 61
Hpy99I CGWCG 1 cut(s) 452
HpyAV CCTTC 1 cut(s) 483
HpyCH4III ACNGT 3 cut(s) 452, 559, 606
HpyCH4V TGCA 4 cut(s) 21, 234, 377, 611
HpyF3I CTNAG 2 cut(s) 34, 647
Hsp92II CATG 3 cut(s) 125, 398, 443
HspAI GCGC 2 cut(s) 77, 288
KpnI GGTACC 1 cut(s) 276
Kzo9I GATC 4 cut(s) 331, 361, 390, 458
LmnI GCTCC 1 cut(s) 642
LpnPI CCDG 9 cut(s) 77, 183, 236, 251, 264, 500, 571, 595, 620
Lsp1109I GCAGC 1 cut(s) 433
LweI GCATC 2 cut(s) 243, 400
MaeIII GTNAC 3 cut(s) 94, 124, 553
MalI GATC 4 cut(s) 333, 363, 392, 460
MboI GATC 4 cut(s) 331, 361, 390, 458
MboII GAAGA 5 cut(s) 134, 161, 337, 350, 476
MluCI AATT 5 cut(s) 160, 217, 431, 496, 571
MlyI GAGTC 2 cut(s) 182, 533
MnlI CCTC 2 cut(s) 144, 654
Mph1103I ATGCAT 1 cut(s) 236
MseI TTAA 1 cut(s) 104
MspA1I CMGCKG 1 cut(s) 424
Mva1269I GAATGC 1 cut(s) 236
MvnI CGCG 2 cut(s) 288, 290
NdeII GATC 4 cut(s) 331, 361, 390, 458
NlaIII CATG 3 cut(s) 125, 398, 443
NlaIV GGNNCC 3 cut(s) 10, 274, 644
NmuCI GTSAC 2 cut(s) 94, 553
NsiI ATGCAT 1 cut(s) 236
NspV TTCGAA 1 cut(s) 575
PctI GAATGC 1 cut(s) 236
PfeI GAWTC 2 cut(s) 326, 577
PkrI GCNGC 1 cut(s) 423
PleI GAGTC 2 cut(s) 182, 532
PpsI GAGTC 2 cut(s) 182, 532
PspFI CCCAGC 1 cut(s) 581
PspN4I GGNNCC 3 cut(s) 10, 274, 644
PspPI GGNCC 1 cut(s) 8
PvuII CAGCTG 1 cut(s) 424
RsaI GTAC 2 cut(s) 110, 274
RsaNI GTAC 2 cut(s) 109, 273
SaqAI TTAA 1 cut(s) 104
SatI GCNGC 1 cut(s) 422
Sau3AI GATC 4 cut(s) 331, 361, 390, 458
Sau96I GGNCC 1 cut(s) 8
SchI GAGTC 2 cut(s) 182, 533
SetI ASST 7 cut(s) 40, 91, 426, 475, 489, 601, 653
SfaNI GCATC 2 cut(s) 243, 400
SfcI CTRYAG 2 cut(s) 528, 652
SfuI TTCGAA 1 cut(s) 575
SinI GGWCC 1 cut(s) 8
Sse9I AATT 5 cut(s) 160, 217, 431, 496, 571
SsiI CCGC 4 cut(s) 276, 286, 533, 640
SspI AATATT 1 cut(s) 617
StyI CCWWGG 2 cut(s) 12, 268
TaaI ACNGT 3 cut(s) 452, 559, 606
TaqI TCGA 3 cut(s) 256, 483, 575
TasI AATT 5 cut(s) 160, 217, 431, 496, 571
TatI WGTACW 1 cut(s) 108
TfiI GAWTC 2 cut(s) 326, 577
Tru1I TTAA 1 cut(s) 104
Tru9I TTAA 1 cut(s) 104
TscAI CASTG 2 cut(s) 355, 609
TseFI GTSAC 2 cut(s) 94, 553
TseI GCWGC 1 cut(s) 421
Tsp45I GTSAC 2 cut(s) 94, 553
TspDTI ATGAA 2 cut(s) 164, 411
TspRI CASTG 2 cut(s) 355, 609
VpaK11BI GGWCC 1 cut(s) 8
XapI RAATTY 2 cut(s) 496, 571
Zsp2I ATGCAT 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.