Rh4CG325200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0016830
Physical Location & Seq
Reverse (-)
58651245 .. 58652018
774 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG325200.1

Sequence Viewer

Length: 603 bp
ATGGGAAATTGTGTTCCGGTTCACAAGTCCAAAGACTCGGCCTCAAAACTCCGATGCTCAAGCTATTCAAGGACGGAATGCGTCCTGATTCAAAAGCCTATTCAAGAAAGCGCAGACTGTACACGGAGGCCTCGTGTGACTGGTTTTCCAGAACTAAGTGATGAAGAGGATGTACATTTTTGTTCTGACCCCTGGGTAGAATCTGATGTTGAAGATTTCTTCAGTGTTAACAACGATCTTACCCCATCTAGAAATGATAGTACTCCAATCCACAAAAACAGTGAAATTATAGAAGTGGAAACCCCCAACTTGATAAACCACAAAAACAAATCCAAGGTTCAACCGGTTAAGCAACTGATTGAATTGTTTCATGAAAGCTTTACCGCTGATGAGGCCGGTGGCCATCAACAGATGCATTATGGGTCAGAAGCTGAATCTTCCCTATCTGACCTACCACGAAGTTCAGCGAATGTAAGCCCCTATCATTATTCGGTGGCAAATTTAGTTTCGGGTGCCAGGACAAGAAAAGCTACACAGTTTGCGCAATGCTTCCTTCCAAAGTTGGTGCAGATCATAAGCTTCGGTCAGAGGAAGAGATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

200

Amino Acids

22.46

Weight (kDa)

6.24

Isoelectric Point (pI)

64.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016830)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23980
malus_domestica MD16G1117200.v1.1
prunus_persica Prupe.1G229000_v2.0.a1
pyrus_communis pycom16g10040
rosa_chinensis RchiOBHm_Chr4g0430641
rosa_laevigata RLG00000006960
rosa_multiflora Rmu_sc0000131.1_g000014
rosa_roxburghii Rroxscaffold_5G00372390
rosa_rugosa Rorug04G0245000
rosa_samantha Rh4AG301000 Rh4BG308100 Rh4CG325200 Rh4DG305700
rosa_wichuraiana Rw4G026120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 543
AccB1I GGYRCC 1 cut(s) 512
AciI CCGC 1 cut(s) 384
AcoI YGGCCR 1 cut(s) 400
AcsI RAATTY 1 cut(s) 499
AcuI CTGAAG 1 cut(s) 205
AfaI GTAC 3 cut(s) 121, 174, 262
AgeI ACCGGT 1 cut(s) 343
AgsI TTSAA 6 cut(s) 69, 92, 104, 212, 341, 362
AjnI CCWGG 2 cut(s) 191, 515
AloI GAACNNNNNNTCC 1 cut(s) 29
AluBI AGCT 5 cut(s) 63, 378, 431, 530, 579
AluI AGCT 5 cut(s) 63, 378, 431, 530, 579
AlwNI CAGNNNCTG 1 cut(s) 431
AoxI GGCC 4 cut(s) 39, 128, 393, 400
ApoI RAATTY 1 cut(s) 499
AsiGI ACCGGT 1 cut(s) 343
Asp700I GAANNNNTTC 1 cut(s) 366
AspLEI GCGC 2 cut(s) 113, 544
BalI TGGCCA 1 cut(s) 402
BanI GGYRCC 1 cut(s) 512
BarI GAAGNNNNNNTAC 2 cut(s) 156, 188
BauI CACGAG 1 cut(s) 132
BccI CCATC 2 cut(s) 253, 411
BciT130I CCWGG 2 cut(s) 193, 517
BfaI CTAG 1 cut(s) 249
BmcAI AGTACT 1 cut(s) 262
Bme1390I CCNGG 2 cut(s) 193, 517
BmiI GGNNCC 1 cut(s) 514
BmrFI CCNGG 2 cut(s) 193, 517
BmsI GCATC 3 cut(s) 44, 402, 587
BpuEI CTTGAG 1 cut(s) 43
BsaJI CCNNGG 3 cut(s) 191, 192, 333
BsaWI WCCGGW 2 cut(s) 16, 343
BsaXI ACNNNNNCTCC 2 cut(s) 118, 148
Bse118I RCCGGY 2 cut(s) 343, 395
Bse1I ACTGG 1 cut(s) 145
Bse3DI GCAATG 1 cut(s) 551
BseBI CCWGG 2 cut(s) 193, 517
BseDI CCNNGG 3 cut(s) 191, 192, 333
BseGI GGATG 1 cut(s) 175
BseMI GCAATG 1 cut(s) 551
BseNI ACTGG 1 cut(s) 145
BsgI GTGCAG 1 cut(s) 587
BshFI GGCC 4 cut(s) 41, 130, 395, 402
BshNI GGYRCC 1 cut(s) 512
BshTI ACCGGT 1 cut(s) 343
BsiSI CCGG 3 cut(s) 17, 344, 396
BsmI GAATGC 1 cut(s) 83
BsnI GGCC 4 cut(s) 41, 130, 395, 402
Bsp1407I TGTACA 2 cut(s) 119, 172
Bsp143I GATC 2 cut(s) 235, 570
BspACI CCGC 1 cut(s) 384
BspANI GGCC 4 cut(s) 41, 130, 395, 402
BspHI TCATGA 1 cut(s) 370
BspLI GGNNCC 1 cut(s) 514
BspT107I GGYRCC 1 cut(s) 512
BsrDI GCAATG 1 cut(s) 551
BsrFI RCCGGY 2 cut(s) 343, 395
BsrGI TGTACA 2 cut(s) 119, 172
BsrI ACTGG 1 cut(s) 145
BssAI RCCGGY 2 cut(s) 343, 395
BssECI CCNNGG 3 cut(s) 191, 192, 333
BssMI GATC 2 cut(s) 235, 570
BssSI CACGAG 1 cut(s) 132
BssT1I CCWWGG 1 cut(s) 333
Bst2BI CACGAG 1 cut(s) 132
Bst2UI CCWGG 2 cut(s) 193, 517
Bst4CI ACNGT 3 cut(s) 119, 281, 537
Bst6I CTCTTC 2 cut(s) 159, 587
BstAUI TGTACA 2 cut(s) 119, 172
BstDEI CTNAG 1 cut(s) 155
BstF5I GGATG 1 cut(s) 175
BstHHI GCGC 2 cut(s) 113, 544
BstKTI GATC 2 cut(s) 238, 573
BstMBI GATC 2 cut(s) 235, 570
BstMWI GCNNNNNNNGC 1 cut(s) 392
BstNI CCWGG 2 cut(s) 193, 517
BstSCI CCNGG 2 cut(s) 191, 515
BsuRI GGCC 4 cut(s) 41, 130, 395, 402
BtsCI GGATG 1 cut(s) 175
BtsIMutI CAGTG 2 cut(s) 229, 286
CaiI CAGNNNCTG 1 cut(s) 431
CciI TCATGA 1 cut(s) 370
CfoI GCGC 2 cut(s) 113, 544
Cfr10I RCCGGY 2 cut(s) 343, 395
CseI GACGC 1 cut(s) 70
Csp6I GTAC 3 cut(s) 120, 173, 261
CspAI ACCGGT 1 cut(s) 343
CviAII CATG 1 cut(s) 371
CviQI GTAC 3 cut(s) 120, 173, 261
DdeI CTNAG 1 cut(s) 155
DpnI GATC 2 cut(s) 237, 572
DpnII GATC 2 cut(s) 235, 570
EaeI YGGCCR 1 cut(s) 400
Eam1104I CTCTTC 2 cut(s) 159, 587
EarI CTCTTC 2 cut(s) 159, 587
Eco130I CCWWGG 1 cut(s) 333
Eco147I AGGCCT 1 cut(s) 130
Eco57I CTGAAG 1 cut(s) 205
EcoRII CCWGG 2 cut(s) 191, 515
EcoT14I CCWWGG 1 cut(s) 333
EcoT22I ATGCAT 1 cut(s) 417
ErhI CCWWGG 1 cut(s) 333
FaeI CATG 1 cut(s) 374
FaiI YATR 4 cut(s) 290, 372, 420, 575
FatI CATG 1 cut(s) 370
FokI GGATG 1 cut(s) 182
FspBI CTAG 1 cut(s) 249
FspI TGCGCA 1 cut(s) 543
GlaI GCGC 2 cut(s) 112, 543
HaeIII GGCC 4 cut(s) 41, 130, 395, 402
HapII CCGG 3 cut(s) 17, 344, 396
HgaI GACGC 1 cut(s) 70
HhaI GCGC 2 cut(s) 113, 544
Hin1II CATG 1 cut(s) 374
Hin6I GCGC 2 cut(s) 111, 542
HinP1I GCGC 2 cut(s) 111, 542
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HindIII AAGCTT 2 cut(s) 376, 577
HinfI GANTC 4 cut(s) 35, 88, 200, 434
HpaI GTTAAC 1 cut(s) 229
HpaII CCGG 3 cut(s) 17, 344, 396
Hpy166II GTNNAC 3 cut(s) 22, 122, 229
Hpy188I TCNGA 6 cut(s) 53, 187, 205, 427, 448, 588
Hpy188III TCNNGA 5 cut(s) 85, 104, 149, 249, 371
Hpy8I GTNNAC 3 cut(s) 22, 122, 229
HpyAV CCTTC 1 cut(s) 563
HpyCH4III ACNGT 3 cut(s) 119, 281, 537
HpyCH4V TGCA 2 cut(s) 415, 568
HpyF10VI GCNNNNNNNGC 1 cut(s) 392
HpyF3I CTNAG 1 cut(s) 155
Hsp92II CATG 1 cut(s) 374
HspAI GCGC 2 cut(s) 111, 542
KspAI GTTAAC 1 cut(s) 229
Kzo9I GATC 2 cut(s) 235, 570
LweI GCATC 3 cut(s) 44, 402, 587
MaeI CTAG 1 cut(s) 249
MaeIII GTNAC 1 cut(s) 136
MalI GATC 2 cut(s) 237, 572
MboI GATC 2 cut(s) 235, 570
MboII GAAGA 4 cut(s) 176, 211, 224, 429
MlsI TGGCCA 1 cut(s) 402
MluCI AATT 4 cut(s) 7, 285, 362, 499
MluNI TGGCCA 1 cut(s) 402
MlyI GAGTC 1 cut(s) 29
MnlI CCTC 6 cut(s) 52, 120, 141, 160, 385, 582
Mox20I TGGCCA 1 cut(s) 402
Mph1103I ATGCAT 1 cut(s) 417
MroXI GAANNNNTTC 1 cut(s) 366
MscI TGGCCA 1 cut(s) 402
MseI TTAA 2 cut(s) 228, 348
Msp20I TGGCCA 1 cut(s) 402
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 3 cut(s) 17, 344, 396
MspR9I CCNGG 2 cut(s) 193, 517
Mva1269I GAATGC 1 cut(s) 83
MvaI CCWGG 2 cut(s) 193, 517
MwoI GCNNNNNNNGC 1 cut(s) 392
NdeII GATC 2 cut(s) 235, 570
NlaIII CATG 1 cut(s) 374
NlaIV GGNNCC 1 cut(s) 514
NmeAIII GCCGAG 1 cut(s) 17
NmuCI GTSAC 1 cut(s) 136
NsbI TGCGCA 1 cut(s) 543
NsiI ATGCAT 1 cut(s) 417
PagI TCATGA 1 cut(s) 370
PasI CCCWGGG 1 cut(s) 192
PceI AGGCCT 1 cut(s) 130
PcsI WCGNNNNNNNCGW 1 cut(s) 130
PctI GAATGC 1 cut(s) 83
PdmI GAANNNNTTC 1 cut(s) 366
PfeI GAWTC 3 cut(s) 88, 200, 434
PinAI ACCGGT 1 cut(s) 343
PleI GAGTC 1 cut(s) 29
PpsI GAGTC 1 cut(s) 29
Psp6I CCWGG 2 cut(s) 191, 515
PspGI CCWGG 2 cut(s) 191, 515
PspN4I GGNNCC 1 cut(s) 514
PstNI CAGNNNCTG 1 cut(s) 431
RsaI GTAC 3 cut(s) 121, 174, 262
RsaNI GTAC 3 cut(s) 120, 173, 261
SaqAI TTAA 2 cut(s) 228, 348
Sau3AI GATC 2 cut(s) 235, 570
ScaI AGTACT 1 cut(s) 262
SchI GAGTC 1 cut(s) 29
ScrFI CCNGG 2 cut(s) 193, 517
SetI ASST 7 cut(s) 65, 339, 380, 433, 453, 532, 581
SfaNI GCATC 3 cut(s) 44, 402, 587
SmlI CTYRAG 1 cut(s) 58
SmoI CTYRAG 1 cut(s) 58
Sse9I AATT 4 cut(s) 7, 285, 362, 499
SseBI AGGCCT 1 cut(s) 130
SsiI CCGC 1 cut(s) 384
SspMI CTAG 1 cut(s) 249
StuI AGGCCT 1 cut(s) 130
StyD4I CCNGG 2 cut(s) 191, 515
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 3 cut(s) 119, 281, 537
TaqII GACCGA 1 cut(s) 572
TasI AATT 4 cut(s) 7, 285, 362, 499
TatI WGTACW 3 cut(s) 119, 172, 260
TfiI GAWTC 3 cut(s) 88, 200, 434
Tru1I TTAA 2 cut(s) 228, 348
Tru9I TTAA 2 cut(s) 228, 348
TscAI CASTG 2 cut(s) 229, 286
TseFI GTSAC 1 cut(s) 136
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 3 cut(s) 177, 359, 387
TspGWI ACGGA 2 cut(s) 89, 139
TspRI CASTG 2 cut(s) 229, 286
XapI RAATTY 1 cut(s) 499
XbaI TCTAGA 1 cut(s) 248
XmnI GAANNNNTTC 1 cut(s) 366
XspI CTAG 1 cut(s) 249
ZrmI AGTACT 1 cut(s) 262
Zsp2I ATGCAT 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.