MD16G1162300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014199
Physical Location & Seq
Forward (+)
13247142 .. 13249786
2645 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1162300.v1.1.491

Sequence Viewer

Length: 927 bp
ATGATCACCACCACTCTCCATCTCTCGCTCCCTCATCCTCCGTCTTTTCACTCCCTGGCGCGCGGCGCGTGCTCCCACCGCCAACCTCTCTTCCTATCCCACCTCACCTTCCCGACCTTACACATCTCAAAAAACACTCCACTTCATCGGCTTCGACGATCATCTCCGATCGCCATGTCCTCCGATTCCGGCCGATCAAGCTCGCCGGCGACCCAACCGCCGCCAATGGAACTCAACGACGAGTCGGATTTCGAAACCATCGTCTCACCCGACGGCTACATCTCCATCTGTGGCTTCGGTTCCCTCCTCTCCGAGAGAAGCGCGAGGAGTACGTTTCCGGAGCTGATCAACTTCAGAGAGTCGCGGTTGAACGGCTTCCGGCGAGTGTATGCTCACATGGCTCCCATTTTCTTCGAGCGTGGCATTGCCAAGCCTGAGACCAAGGAGATTGCGAGCTTGAGTGTAGAGCCATTTGAAGGGGAAAACCTTATAGTAACAGTGTTTGAGATTAAAAAATCAGAGATTCCAGCTTTTATCAAAAGGGAGCCGGAATTTCGATTCCTATCTGTTGTGCCTGAATCACTTGATGGGAAGCCCTATGATAAGCGAGCGGTTCTTTGTGCTCGTTATAGTGATGAAGAATTTTTCAGAGTTAGATGCAAAGGAAGTAAGGAGATGTATTATGAGCTATTTGGACGATATAATATTGATAAGATTTGGCGAGATGACATCTTACCTTGTCGCACTTACCTTCGGCATTGTGTGTTAGCAGCAAAGAATCTGAGCGAGGCAGCATACAACAACTTCTTGGATCATACTTTTCTTGGAGACCGTAAAACGACTATCCGTGAGTACTTGGCTACAACAGGTTCGGGCATCATGGAAGAAGAGCCTCCAGAATCCCTCAAGACCCGTTATGGCGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMD

Protein Analysis

309

Amino Acids

35.11

Weight (kDa)

7.68

Isoelectric Point (pI)

55.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014199)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16060
fragaria_vesca FvH4_4g18820 FvH4_4g18820
malus_domestica MD16G1162300.v1.1
prunus_persica Prupe.1G129900_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0423631
rosa_laevigata RLG00000007540
rosa_multiflora Rmu_sc0000464.1_g000016 Rmu_sc0038723.1_g000001
rosa_roxburghii Rroxscaffold_5G00365840
rosa_rugosa Rorug04G0189200 Rorug04G0189200
rosa_samantha Rh4AG248800 Rh4BG254100 Rh4CG262100 Rh4DG249100
rosa_wichuraiana Rw4G021310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 611
AccII CGCG 5 cut(s) 61, 63, 68, 323, 364
AccIII TCCGGA 1 cut(s) 337
AciI CCGC 7 cut(s) 63, 79, 218, 221, 364, 611, 921
AclWI GGATC 1 cut(s) 819
AcoI YGGCCR 1 cut(s) 190
AcsI RAATTY 2 cut(s) 551, 641
AcuI CTGAAG 1 cut(s) 337
AfaI GTAC 2 cut(s) 331, 854
AfiI CCNNNNNNNGG 1 cut(s) 476
AgsI TTSAA 2 cut(s) 370, 476
AjnI CCWGG 1 cut(s) 54
AluBI AGCT 5 cut(s) 201, 343, 456, 530, 688
AluI AGCT 5 cut(s) 201, 343, 456, 530, 688
Alw21I GWGCWC 2 cut(s) 74, 625
Alw26I GTCTC 3 cut(s) 268, 431, 822
AlwI GGATC 1 cut(s) 819
Aor13HI TCCGGA 1 cut(s) 337
AoxI GGCC 1 cut(s) 190
ApeKI GCWGC 2 cut(s) 770, 791
ApoI RAATTY 2 cut(s) 551, 641
ArsI GACNNNNNNTTYG 2 cut(s) 246, 278
Asp700I GAANNNNTTC 1 cut(s) 374
AspLEI GCGC 4 cut(s) 61, 63, 68, 323
AsuHPI GGTGA 2 cut(s) 97, 258
AsuII TTCGAA 1 cut(s) 252
Bbv12I GWGCWC 2 cut(s) 74, 625
BbvI GCAGC 2 cut(s) 782, 803
BccI CCATC 4 cut(s) 27, 266, 293, 581
BceAI ACGGC 2 cut(s) 289, 388
BciT130I CCWGG 1 cut(s) 56
BclI TGATCA 2 cut(s) 3, 345
BcoDI GTCTC 3 cut(s) 268, 431, 822
BisI GCNGC 4 cut(s) 64, 221, 771, 792
BlsI GCNGC 4 cut(s) 65, 222, 772, 793
BmcAI AGTACT 1 cut(s) 854
Bme1390I CCNGG 1 cut(s) 56
BmiI GGNNCC 3 cut(s) 301, 402, 546
BmrFI CCNGG 1 cut(s) 56
BmsI GCATC 2 cut(s) 647, 885
BpmI CTGGAG 1 cut(s) 879
Bpu14I TTCGAA 1 cut(s) 252
BpuEI CTTGAG 2 cut(s) 478, 890
BsaBI GATNNNNATC 1 cut(s) 562
BsaI GGTCTC 2 cut(s) 431, 822
BsaJI CCNNGG 2 cut(s) 54, 441
BsaWI WCCGGW 1 cut(s) 337
Bsc4I CCNNNNNNNGG 1 cut(s) 476
Bse118I RCCGGY 1 cut(s) 205
Bse3DI GCAATG 1 cut(s) 423
Bse8I GATNNNNATC 1 cut(s) 562
BseAI TCCGGA 1 cut(s) 337
BseBI CCWGG 1 cut(s) 56
BseDI CCNNGG 2 cut(s) 54, 441
BseGI GGATG 1 cut(s) 34
BseJI GATNNNNATC 1 cut(s) 562
BseLI CCNNNNNNNGG 1 cut(s) 476
BseMI GCAATG 1 cut(s) 423
BseMII CTCAG 2 cut(s) 426, 773
BsePI GCGCGC 1 cut(s) 59
BseRI GAGGAG 2 cut(s) 296, 340
BseX3I CGGCCG 1 cut(s) 190
BseXI GCAGC 2 cut(s) 782, 803
Bsh1236I CGCG 5 cut(s) 61, 63, 68, 323, 364
Bsh1285I CGRYCG 2 cut(s) 171, 193
BshFI GGCC 1 cut(s) 192
BsiEI CGRYCG 2 cut(s) 171, 193
BsiHKAI GWGCWC 2 cut(s) 74, 625
BsiSI CCGG 5 cut(s) 189, 206, 338, 379, 548
BslI CCNNNNNNNGG 1 cut(s) 476
BsmAI GTCTC 3 cut(s) 268, 431, 822
BsmBI CGTCTC 1 cut(s) 268
BsnI GGCC 1 cut(s) 192
Bso31I GGTCTC 2 cut(s) 431, 822
Bsp119I TTCGAA 1 cut(s) 252
Bsp1286I GDGCHC 2 cut(s) 74, 625
Bsp13I TCCGGA 1 cut(s) 337
Bsp143I GATC 6 cut(s) 3, 158, 168, 194, 345, 811
BspACI CCGC 7 cut(s) 63, 79, 218, 221, 364, 611, 921
BspANI GGCC 1 cut(s) 192
BspCNI CTCAG 2 cut(s) 427, 774
BspEI TCCGGA 1 cut(s) 337
BspFNI CGCG 5 cut(s) 61, 63, 68, 323, 364
BspLI GGNNCC 3 cut(s) 301, 402, 546
BspPI GGATC 1 cut(s) 819
BspQI GCTCTTC 1 cut(s) 882
BspT104I TTCGAA 1 cut(s) 252
BspTNI GGTCTC 2 cut(s) 431, 822
BsrBI CCGCTC 1 cut(s) 611
BsrDI GCAATG 1 cut(s) 423
BsrFI RCCGGY 1 cut(s) 205
BssAI RCCGGY 1 cut(s) 205
BssECI CCNNGG 2 cut(s) 54, 441
BssHII GCGCGC 1 cut(s) 59
BssMI GATC 6 cut(s) 3, 158, 168, 194, 345, 811
BssT1I CCWWGG 1 cut(s) 441
Bst2UI CCWGG 1 cut(s) 56
Bst4CI ACNGT 2 cut(s) 499, 833
Bst6I CTCTTC 2 cut(s) 95, 882
BstBI TTCGAA 1 cut(s) 252
BstC8I GCNNGC 6 cut(s) 61, 70, 203, 207, 454, 609
BstDEI CTNAG 2 cut(s) 435, 782
BstF5I GGATG 1 cut(s) 34
BstFNI CGCG 5 cut(s) 61, 63, 68, 323, 364
BstHHI GCGC 4 cut(s) 61, 63, 68, 323
BstKTI GATC 6 cut(s) 6, 161, 171, 197, 348, 814
BstMAI GTCTC 3 cut(s) 268, 431, 822
BstMBI GATC 6 cut(s) 3, 158, 168, 194, 345, 811
BstMCI CGRYCG 2 cut(s) 171, 193
BstMWI GCNNNNNNNGC 5 cut(s) 65, 69, 78, 198, 398
BstNI CCWGG 1 cut(s) 56
BstSCI CCNGG 1 cut(s) 54
BstUI CGCG 5 cut(s) 61, 63, 68, 323, 364
BstV1I GCAGC 2 cut(s) 782, 803
BstZI CGGCCG 1 cut(s) 190
BsuRI GGCC 1 cut(s) 192
BtsCI GGATG 1 cut(s) 34
BtsIMutI CAGTG 1 cut(s) 504
Cac8I GCNNGC 6 cut(s) 61, 70, 203, 207, 454, 609
CfoI GCGC 4 cut(s) 61, 63, 68, 323
Cfr10I RCCGGY 1 cut(s) 205
Csp6I GTAC 2 cut(s) 330, 853
CviAII CATG 3 cut(s) 175, 397, 880
CviQI GTAC 2 cut(s) 330, 853
DdeI CTNAG 2 cut(s) 435, 782
DpnI GATC 6 cut(s) 5, 160, 170, 196, 347, 813
DpnII GATC 6 cut(s) 3, 158, 168, 194, 345, 811
EaeI YGGCCR 1 cut(s) 190
EagI CGGCCG 1 cut(s) 190
Eam1104I CTCTTC 2 cut(s) 95, 882
EarI CTCTTC 2 cut(s) 95, 882
EclXI CGGCCG 1 cut(s) 190
Eco130I CCWWGG 1 cut(s) 441
Eco31I GGTCTC 2 cut(s) 431, 822
Eco52I CGGCCG 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 54
EcoT14I CCWWGG 1 cut(s) 441
ErhI CCWWGG 1 cut(s) 441
Esp3I CGTCTC 1 cut(s) 268
FaeI CATG 3 cut(s) 178, 400, 883
FatI CATG 3 cut(s) 174, 396, 879
FbaI TGATCA 2 cut(s) 3, 345
Fnu4HI GCNGC 4 cut(s) 64, 221, 771, 792
FokI GGATG 1 cut(s) 21
Fsp4HI GCNGC 4 cut(s) 64, 221, 771, 792
GlaI GCGC 4 cut(s) 60, 62, 67, 322
GluI GCNGC 4 cut(s) 64, 221, 771, 792
GsuI CTGGAG 1 cut(s) 879
HaeIII GGCC 1 cut(s) 192
HapII CCGG 5 cut(s) 189, 206, 338, 379, 548
HhaI GCGC 4 cut(s) 61, 63, 68, 323
Hin1II CATG 3 cut(s) 178, 400, 883
Hin6I GCGC 4 cut(s) 59, 61, 66, 321
HinP1I GCGC 4 cut(s) 59, 61, 66, 321
HinfI GANTC 8 cut(s) 185, 242, 359, 523, 558, 578, 778, 899
HpaII CCGG 5 cut(s) 189, 206, 338, 379, 548
HphI GGTGA 2 cut(s) 97, 258
Hpy188I TCNGA 8 cut(s) 168, 184, 247, 313, 356, 520, 650, 783
Hpy188III TCNNGA 4 cut(s) 112, 338, 896, 907
Hpy99I CGWCG 3 cut(s) 159, 242, 275
HpyAV CCTTC 3 cut(s) 118, 470, 761
HpyCH4III ACNGT 2 cut(s) 499, 833
HpyCH4IV ACGT 1 cut(s) 332
HpyCH4V TGCA 1 cut(s) 660
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 69, 78, 198, 398
HpyF3I CTNAG 2 cut(s) 435, 782
HpySE526I ACGT 1 cut(s) 332
Hsp92II CATG 3 cut(s) 178, 400, 883
HspAI GCGC 4 cut(s) 59, 61, 66, 321
Kpn2I TCCGGA 1 cut(s) 337
KroI GCCGGC 1 cut(s) 205
KroNI GCCGGC 1 cut(s) 207
Ksp22I TGATCA 2 cut(s) 3, 345
Kzo9I GATC 6 cut(s) 3, 158, 168, 194, 345, 811
LguI GCTCTTC 1 cut(s) 882
LmnI GCTCC 5 cut(s) 33, 77, 340, 406, 544
Lsp1109I GCAGC 2 cut(s) 782, 803
LweI GCATC 2 cut(s) 647, 885
MaeII ACGT 1 cut(s) 332
MaeIII GTNAC 1 cut(s) 493
MalI GATC 6 cut(s) 5, 160, 170, 196, 347, 813
MbiI CCGCTC 1 cut(s) 611
MboI GATC 6 cut(s) 3, 158, 168, 194, 345, 811
MboII GAAGA 5 cut(s) 82, 403, 650, 896, 899
MhlI GDGCHC 2 cut(s) 74, 625
MluCI AATT 2 cut(s) 551, 641
MlyI GAGTC 2 cut(s) 251, 368
MmeI TCCRAC 1 cut(s) 225
MreI CGCCGGCG 1 cut(s) 205
MroI TCCGGA 1 cut(s) 337
MroNI GCCGGC 1 cut(s) 205
MroXI GAANNNNTTC 1 cut(s) 374
MseI TTAA 2 cut(s) 510, 925
MspI CCGG 5 cut(s) 189, 206, 338, 379, 548
MspR9I CCNGG 1 cut(s) 56
MteI GCGCNGCGC 1 cut(s) 64
MvaI CCWGG 1 cut(s) 56
MvnI CGCG 5 cut(s) 61, 63, 68, 323, 364
MwoI GCNNNNNNNGC 5 cut(s) 65, 69, 78, 198, 398
NaeI GCCGGC 1 cut(s) 207
NdeII GATC 6 cut(s) 3, 158, 168, 194, 345, 811
NgoMIV GCCGGC 1 cut(s) 205
NlaIII CATG 3 cut(s) 178, 400, 883
NlaIV GGNNCC 3 cut(s) 301, 402, 546
NspV TTCGAA 1 cut(s) 252
PauI GCGCGC 1 cut(s) 59
PciSI GCTCTTC 1 cut(s) 882
PcsI WCGNNNNNNNCGW 3 cut(s) 154, 258, 267
PdiI GCCGGC 1 cut(s) 207
PdmI GAANNNNTTC 1 cut(s) 374
PfeI GAWTC 6 cut(s) 185, 523, 558, 578, 778, 899
PkrI GCNGC 4 cut(s) 65, 222, 772, 793
Ple19I CGATCG 1 cut(s) 171
PleI GAGTC 2 cut(s) 250, 367
PpsI GAGTC 2 cut(s) 250, 367
Psp6I CCWGG 1 cut(s) 54
PspGI CCWGG 1 cut(s) 54
PspN4I GGNNCC 3 cut(s) 301, 402, 546
PteI GCGCGC 1 cut(s) 59
PvuI CGATCG 1 cut(s) 171
RsaI GTAC 2 cut(s) 331, 854
RsaNI GTAC 2 cut(s) 330, 853
SapI GCTCTTC 1 cut(s) 882
SaqAI TTAA 2 cut(s) 510, 925
SatI GCNGC 4 cut(s) 64, 221, 771, 792
Sau3AI GATC 6 cut(s) 3, 158, 168, 194, 345, 811
ScaI AGTACT 1 cut(s) 854
SchI GAGTC 2 cut(s) 251, 368
ScrFI CCNGG 1 cut(s) 56
SduI GDGCHC 2 cut(s) 74, 625
SfaNI GCATC 2 cut(s) 647, 885
SfuI TTCGAA 1 cut(s) 252
SgrAI CRCCGGYG 1 cut(s) 205
SmlI CTYRAG 2 cut(s) 457, 905
SmoI CTYRAG 2 cut(s) 457, 905
Sse9I AATT 2 cut(s) 551, 641
SsiI CCGC 7 cut(s) 63, 79, 218, 221, 364, 611, 921
SspI AATATT 1 cut(s) 706
StyD4I CCNGG 1 cut(s) 54
StyI CCWWGG 1 cut(s) 441
TaaI ACNGT 2 cut(s) 499, 833
TaiI ACGT 1 cut(s) 335
TaqI TCGA 4 cut(s) 154, 252, 414, 556
TasI AATT 2 cut(s) 551, 641
TatI WGTACW 1 cut(s) 852
TauI GCSGC 2 cut(s) 66, 223
TfiI GAWTC 6 cut(s) 185, 523, 558, 578, 778, 899
Tru1I TTAA 2 cut(s) 510, 925
Tru9I TTAA 2 cut(s) 510, 925
TscAI CASTG 1 cut(s) 504
TseI GCWGC 2 cut(s) 770, 791
TspDTI ATGAA 2 cut(s) 134, 651
TspGWI ACGGA 2 cut(s) 30, 836
TspRI CASTG 1 cut(s) 504
XapI RAATTY 2 cut(s) 551, 641
XmnI GAANNNNTTC 1 cut(s) 374
ZrmI AGTACT 1 cut(s) 854
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.