Rw4G021310

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014199
Physical Location & Seq
Reverse (-)
45968204 .. 45970639
2436 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G021310.1

Sequence Viewer

Length: 939 bp
ATGACTACCACTGCTCTCCGTCTCTCCCTCCTTCCATCTCCTCAATCACTCGCTCCCGGCGCGTGTCCTCACCGCCAACCTCTCTCCCACTCCCACTGCCACCTCACCTTCCCAACCCTCCACAGCTCCACCCACACTCCACTCCTCCGCTCCTCCCATCACCGCCATCCATCTCCGGTCGCCATGTCCTCCGATTCCGGCCGATCAGTCTCTCCGGCGACTCAGCCACCCCAACCGATCGAGCTCAACGACGAGTCCGAATTCGAAACCATCGTCTCGCCCGACGGTCACATCTCCATCTGCGGCTTCGGCTCTCTCCTCTCCGAGAGAAGCGCGAGGAGTACATTTCCGGAGCTGATCAACTTCAGAGTGGCGCGGTTGAACGGATTCCGGCGAGTCTTCGCTCACATGGCTCCGATTTTCTTCGAGCGTGGCATTGCCAAGCCTGAAACCAAGGAGATTTCGAGTTTGAGTGTGGAGCCTTATGAAGGCGAAAGCCTTATAGTGACGGTGTTTGAGATTGAAAAATCGGAGATTCCGGCTTTTATCAAGAGGGAGCCTGAATTTCGATTCCTGGCGGTTCTGCCTGAAAGCCTTGATGGGAAGCCATTTGATAATCGAGCGGTGCTTTGTGCTCGTTATAGTGATGAAGAATTTTTCAGAGTCAGATGCAAAGGAAGTAAGGAAATGTATTTTGAGCTATTTGGGCGATATGACATAGATAAGATTTGGAGGGATGATATCTTACCTTGCCGAACTTACCTTCGTCACTGTGTGTTAGCAGCAAAGAATCTCAACGATATAGCATACAACAACTTCTTGGATCACACTTTACTTGGAGACCGTAAAACAACCATCCGCAAGTACTTGGCTACAACAGGTTCGGGAATCATGGAAGAAGAGCCTCCGGAATCCCTCAAGATCCGTTACGGTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMD

Protein Analysis

312

Amino Acids

35.26

Weight (kDa)

6.68

Isoelectric Point (pI)

61.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014199)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16060
fragaria_vesca FvH4_4g18820 FvH4_4g18820
malus_domestica MD16G1162300.v1.1
prunus_persica Prupe.1G129900_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0423631
rosa_laevigata RLG00000007540
rosa_multiflora Rmu_sc0000464.1_g000016 Rmu_sc0038723.1_g000001
rosa_roxburghii Rroxscaffold_5G00365840
rosa_rugosa Rorug04G0189200 Rorug04G0189200
rosa_samantha Rh4AG248800 Rh4BG254100 Rh4CG262100 Rh4DG249100
rosa_wichuraiana Rw4G021310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 150, 623
AccII CGCG 3 cut(s) 62, 335, 376
AccIII TCCGGA 2 cut(s) 349, 907
AciI CCGC 8 cut(s) 73, 148, 163, 303, 376, 578, 623, 859
AclWI GGATC 2 cut(s) 831, 916
AcoI YGGCCR 1 cut(s) 199
AcsI RAATTY 3 cut(s) 260, 563, 653
AcuI CTGAAG 1 cut(s) 349
AdeI CACNNNGTG 1 cut(s) 775
AfaI GTAC 2 cut(s) 343, 866
AfiI CCNNNNNNNGG 1 cut(s) 488
AgsI TTSAA 2 cut(s) 382, 524
AjnI CCWGG 1 cut(s) 573
AluBI AGCT 4 cut(s) 126, 244, 355, 700
AluI AGCT 4 cut(s) 126, 244, 355, 700
Alw21I GWGCWC 2 cut(s) 246, 637
Alw26I GTCTC 4 cut(s) 26, 214, 280, 834
AlwI GGATC 2 cut(s) 831, 916
Aor13HI TCCGGA 2 cut(s) 349, 907
AoxI GGCC 1 cut(s) 199
ApeKI GCWGC 1 cut(s) 782
ApoI RAATTY 3 cut(s) 260, 563, 653
ArsI GACNNNNNNTTYG 2 cut(s) 258, 290
Asp700I GAANNNNTTC 1 cut(s) 386
AspLEI GCGC 3 cut(s) 62, 335, 376
AsuC2I CCSGG 1 cut(s) 57
AsuHPI GGTGA 3 cut(s) 62, 97, 152
AsuII TTCGAA 1 cut(s) 264
BanII GRGCYC 1 cut(s) 246
BbsI GAAGAC 1 cut(s) 391
Bbv12I GWGCWC 2 cut(s) 246, 637
BbvI GCAGC 1 cut(s) 794
BccI CCATC 8 cut(s) 43, 165, 174, 178, 278, 305, 593, 863
BciT130I CCWGG 1 cut(s) 575
BclI TGATCA 1 cut(s) 357
BcnI CCSGG 1 cut(s) 57
BcoDI GTCTC 4 cut(s) 26, 214, 280, 834
BisI GCNGC 2 cut(s) 304, 783
BlsI GCNGC 2 cut(s) 305, 784
BmcAI AGTACT 1 cut(s) 866
Bme1390I CCNGG 2 cut(s) 57, 575
BmiI GGNNCC 3 cut(s) 414, 480, 558
BmrFI CCNGG 2 cut(s) 57, 575
BmsI GCATC 1 cut(s) 659
BpiI GAAGAC 1 cut(s) 391
Bpu14I TTCGAA 1 cut(s) 264
BpuEI CTTGAG 1 cut(s) 902
BpuMI CCSGG 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 834
BsaJI CCNNGG 1 cut(s) 453
BsaWI WCCGGW 3 cut(s) 175, 349, 907
BsaXI ACNNNNNCTCC 2 cut(s) 121, 151
Bsc4I CCNNNNNNNGG 1 cut(s) 488
Bse3DI GCAATG 1 cut(s) 435
BseAI TCCGGA 2 cut(s) 349, 907
BseBI CCWGG 1 cut(s) 575
BseDI CCNNGG 1 cut(s) 453
BseGI GGATG 3 cut(s) 166, 742, 855
BseLI CCNNNNNNNGG 1 cut(s) 488
BseMI GCAATG 1 cut(s) 435
BseMII CTCAG 1 cut(s) 236
BseRI GAGGAG 5 cut(s) 30, 134, 142, 308, 352
BseX3I CGGCCG 1 cut(s) 199
BseXI GCAGC 1 cut(s) 794
Bsh1236I CGCG 3 cut(s) 62, 335, 376
Bsh1285I CGRYCG 3 cut(s) 180, 202, 240
BshFI GGCC 1 cut(s) 201
BsiEI CGRYCG 3 cut(s) 180, 202, 240
BsiHKAI GWGCWC 2 cut(s) 246, 637
BsiSI CCGG 8 cut(s) 57, 176, 198, 215, 350, 391, 539, 908
BslI CCNNNNNNNGG 1 cut(s) 488
BsmAI GTCTC 4 cut(s) 26, 214, 280, 834
BsmBI CGTCTC 2 cut(s) 26, 280
BsnI GGCC 1 cut(s) 201
Bso31I GGTCTC 1 cut(s) 834
Bsp119I TTCGAA 1 cut(s) 264
Bsp1286I GDGCHC 2 cut(s) 246, 637
Bsp13I TCCGGA 2 cut(s) 349, 907
Bsp143I GATC 5 cut(s) 203, 237, 357, 823, 921
BspACI CCGC 8 cut(s) 73, 148, 163, 303, 376, 578, 623, 859
BspANI GGCC 1 cut(s) 201
BspCNI CTCAG 1 cut(s) 235
BspEI TCCGGA 2 cut(s) 349, 907
BspFNI CGCG 3 cut(s) 62, 335, 376
BspLI GGNNCC 3 cut(s) 414, 480, 558
BspPI GGATC 2 cut(s) 831, 916
BspQI GCTCTTC 1 cut(s) 894
BspT104I TTCGAA 1 cut(s) 264
BspTNI GGTCTC 1 cut(s) 834
BsrBI CCGCTC 2 cut(s) 150, 623
BsrDI GCAATG 1 cut(s) 435
BssECI CCNNGG 1 cut(s) 453
BssMI GATC 5 cut(s) 203, 237, 357, 823, 921
BssT1I CCWWGG 1 cut(s) 453
Bst2UI CCWGG 1 cut(s) 575
Bst4CI ACNGT 5 cut(s) 287, 511, 773, 845, 932
Bst6I CTCTTC 1 cut(s) 894
BstBI TTCGAA 1 cut(s) 264
BstDEI CTNAG 1 cut(s) 222
BstENI CCTNNNNNAGG 1 cut(s) 486
BstF5I GGATG 3 cut(s) 166, 742, 855
BstFNI CGCG 3 cut(s) 62, 335, 376
BstHHI GCGC 3 cut(s) 62, 335, 376
BstKTI GATC 5 cut(s) 206, 240, 360, 826, 924
BstMAI GTCTC 4 cut(s) 26, 214, 280, 834
BstMBI GATC 5 cut(s) 203, 237, 357, 823, 921
BstMCI CGRYCG 3 cut(s) 180, 202, 240
BstMWI GCNNNNNNNGC 4 cut(s) 59, 309, 410, 706
BstNI CCWGG 1 cut(s) 575
BstSCI CCNGG 2 cut(s) 55, 573
BstUI CGCG 3 cut(s) 62, 335, 376
BstV1I GCAGC 1 cut(s) 794
BstV2I GAAGAC 1 cut(s) 391
BstX2I RGATCY 1 cut(s) 921
BstYI RGATCY 1 cut(s) 921
BstZI CGGCCG 1 cut(s) 199
BsuRI GGCC 1 cut(s) 201
BtsCI GGATG 3 cut(s) 166, 742, 855
BtsI GCAGTG 2 cut(s) 9, 94
BtsIMutI CAGTG 3 cut(s) 9, 94, 769
CfoI GCGC 3 cut(s) 62, 335, 376
Csp6I GTAC 2 cut(s) 342, 865
CviAII CATG 3 cut(s) 184, 409, 892
CviQI GTAC 2 cut(s) 342, 865
DdeI CTNAG 1 cut(s) 222
DpnI GATC 5 cut(s) 205, 239, 359, 825, 923
DpnII GATC 5 cut(s) 203, 237, 357, 823, 921
DraIII CACNNNGTG 1 cut(s) 775
EaeI YGGCCR 1 cut(s) 199
EagI CGGCCG 1 cut(s) 199
Eam1104I CTCTTC 1 cut(s) 894
EarI CTCTTC 1 cut(s) 894
Ecl136II GAGCTC 1 cut(s) 244
EclXI CGGCCG 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 453
Eco24I GRGCYC 1 cut(s) 246
Eco31I GGTCTC 1 cut(s) 834
Eco32I GATATC 1 cut(s) 742
Eco52I CGGCCG 1 cut(s) 199
Eco53kI GAGCTC 1 cut(s) 244
Eco57I CTGAAG 1 cut(s) 349
EcoICRI GAGCTC 1 cut(s) 244
EcoNI CCTNNNNNAGG 1 cut(s) 486
EcoRI GAATTC 1 cut(s) 260
EcoRII CCWGG 1 cut(s) 573
EcoRV GATATC 1 cut(s) 742
EcoT14I CCWWGG 1 cut(s) 453
EcoT38I GRGCYC 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 453
Esp3I CGTCTC 2 cut(s) 26, 280
FaeI CATG 3 cut(s) 187, 412, 895
FatI CATG 3 cut(s) 183, 408, 891
FbaI TGATCA 1 cut(s) 357
Fnu4HI GCNGC 2 cut(s) 304, 783
FokI GGATG 3 cut(s) 153, 749, 842
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 2 cut(s) 304, 783
GlaI GCGC 3 cut(s) 61, 334, 375
GluI GCNGC 2 cut(s) 304, 783
HaeIII GGCC 1 cut(s) 201
HapII CCGG 8 cut(s) 57, 176, 198, 215, 350, 391, 539, 908
HhaI GCGC 3 cut(s) 62, 335, 376
Hin1II CATG 3 cut(s) 187, 412, 895
Hin6I GCGC 3 cut(s) 60, 333, 374
HinP1I GCGC 3 cut(s) 60, 333, 374
HpaII CCGG 8 cut(s) 57, 176, 198, 215, 350, 391, 539, 908
HphI GGTGA 3 cut(s) 62, 97, 152
Hpy188I TCNGA 8 cut(s) 193, 259, 325, 368, 417, 532, 662, 668
Hpy188III TCNNGA 5 cut(s) 350, 550, 885, 908, 919
Hpy99I CGWCG 2 cut(s) 254, 287
HpyAV CCTTC 4 cut(s) 41, 118, 482, 773
HpyCH4III ACNGT 5 cut(s) 287, 511, 773, 845, 932
HpyCH4V TGCA 1 cut(s) 672
HpyF10VI GCNNNNNNNGC 4 cut(s) 59, 309, 410, 706
HpyF3I CTNAG 1 cut(s) 222
Hsp92II CATG 3 cut(s) 187, 412, 895
HspAI GCGC 3 cut(s) 60, 333, 374
Kpn2I TCCGGA 2 cut(s) 349, 907
Ksp22I TGATCA 1 cut(s) 357
Kzo9I GATC 5 cut(s) 203, 237, 357, 823, 921
LguI GCTCTTC 1 cut(s) 894
LmnI GCTCC 7 cut(s) 58, 131, 155, 352, 418, 478, 556
Lsp1109I GCAGC 1 cut(s) 794
LweI GCATC 1 cut(s) 659
MaeIII GTNAC 4 cut(s) 287, 505, 767, 926
MalI GATC 5 cut(s) 205, 239, 359, 825, 923
MbiI CCGCTC 2 cut(s) 150, 623
MboI GATC 5 cut(s) 203, 237, 357, 823, 921
MboII GAAGA 5 cut(s) 391, 415, 662, 908, 911
MflI RGATCY 1 cut(s) 921
MhlI GDGCHC 2 cut(s) 246, 637
MluCI AATT 3 cut(s) 260, 563, 653
MlyI GAGTC 4 cut(s) 214, 263, 405, 672
MroI TCCGGA 2 cut(s) 349, 907
MroXI GAANNNNTTC 1 cut(s) 386
MseI TTAA 1 cut(s) 937
MspI CCGG 8 cut(s) 57, 176, 198, 215, 350, 391, 539, 908
MspR9I CCNGG 2 cut(s) 57, 575
MvaI CCWGG 1 cut(s) 575
MvnI CGCG 3 cut(s) 62, 335, 376
MwoI GCNNNNNNNGC 4 cut(s) 59, 309, 410, 706
NciI CCSGG 1 cut(s) 57
NdeII GATC 5 cut(s) 203, 237, 357, 823, 921
NlaIII CATG 3 cut(s) 187, 412, 895
NlaIV GGNNCC 3 cut(s) 414, 480, 558
NmuCI GTSAC 3 cut(s) 287, 505, 767
NspV TTCGAA 1 cut(s) 264
PciSI GCTCTTC 1 cut(s) 894
PcsI WCGNNNNNNNCGW 4 cut(s) 246, 255, 270, 279
PdmI GAANNNNTTC 1 cut(s) 386
PfeI GAWTC 7 cut(s) 194, 387, 535, 570, 790, 888, 911
PkrI GCNGC 2 cut(s) 305, 784
Ple19I CGATCG 1 cut(s) 240
PleI GAGTC 4 cut(s) 214, 262, 404, 671
PpsI GAGTC 4 cut(s) 214, 262, 404, 671
Psp124BI GAGCTC 1 cut(s) 246
Psp6I CCWGG 1 cut(s) 573
PspGI CCWGG 1 cut(s) 573
PspN4I GGNNCC 3 cut(s) 414, 480, 558
PsuI RGATCY 1 cut(s) 921
PvuI CGATCG 1 cut(s) 240
RsaI GTAC 2 cut(s) 343, 866
RsaNI GTAC 2 cut(s) 342, 865
SacI GAGCTC 1 cut(s) 246
SapI GCTCTTC 1 cut(s) 894
SaqAI TTAA 1 cut(s) 937
SatI GCNGC 2 cut(s) 304, 783
Sau3AI GATC 5 cut(s) 203, 237, 357, 823, 921
ScaI AGTACT 1 cut(s) 866
SchI GAGTC 4 cut(s) 214, 263, 405, 672
ScrFI CCNGG 2 cut(s) 57, 575
SduI GDGCHC 2 cut(s) 246, 637
SfaNI GCATC 1 cut(s) 659
SfuI TTCGAA 1 cut(s) 264
SmlI CTYRAG 1 cut(s) 917
SmoI CTYRAG 1 cut(s) 917
Sse9I AATT 3 cut(s) 260, 563, 653
SsiI CCGC 8 cut(s) 73, 148, 163, 303, 376, 578, 623, 859
SstI GAGCTC 1 cut(s) 246
StyD4I CCNGG 2 cut(s) 55, 573
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 5 cut(s) 287, 511, 773, 845, 932
TaqI TCGA 6 cut(s) 240, 264, 426, 464, 568, 619
TasI AATT 3 cut(s) 260, 563, 653
TatI WGTACW 2 cut(s) 341, 864
TauI GCSGC 1 cut(s) 306
TfiI GAWTC 7 cut(s) 194, 387, 535, 570, 790, 888, 911
Tru1I TTAA 1 cut(s) 937
Tru9I TTAA 1 cut(s) 937
TscAI CASTG 3 cut(s) 16, 101, 776
TseFI GTSAC 3 cut(s) 287, 505, 767
TseI GCWGC 1 cut(s) 782
Tsp45I GTSAC 3 cut(s) 287, 505, 767
TspDTI ATGAA 2 cut(s) 501, 663
TspGWI ACGGA 3 cut(s) 8, 399, 914
TspRI CASTG 3 cut(s) 16, 101, 776
XagI CCTNNNNNAGG 1 cut(s) 486
XapI RAATTY 3 cut(s) 260, 563, 653
XmnI GAANNNNTTC 1 cut(s) 386
ZrmI AGTACT 1 cut(s) 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.