MD16G1174300.v1.1

RING finger and CHY zinc finger domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009916
Physical Location & Seq
Forward (+)
14729724 .. 14732682
2959 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1174300.v1.1.491

Sequence Viewer

Length: 804 bp
ATGGAAGGCTCAGCCAACGAACGTCTTGATTTTGGGAAGATGGGTTACGGATGCAAGCATTACAGAAGAAGATGCCAGATTCGAGCTCCGTGCTGCAACGAGATCCATCCGTGCCGCCATTGTCACAACGAAGCCACGAGCATGTTGAGCAACCCCTTTGATCGGCATGAGCTTGTTCGCTACGATGTGAAACAAGTTGTCTGTTCAGTTTGTGACACAGAGCAGCCGGTTGCAAAAGTTTGTACAAACTGCGGCATCAGTATGGGGGAATACTTCTGTGACATTTGCAAATTCTATGATGATGATACCACGAAAGAACAGTTTCATTGTAATGATTGTGGGATCTGCAGAATCGGGGGTCGTGACAAATTCTTTCACTGCAAGAAATGTGGGTCTTGCTATTCAATTCGCTTACGTGATAATCACTTGTGTGTGGAGAACTCCATGCGTCATCACTGCCCTATTTGTTACGAGTTTCTTTTTGACTCTCTGAAAGACACTACTGTAATGAAATGCGGGCACACAATGCACCGCGAATGTTACAATGAAATGATGCAGCGTGACAAATATTGTTGTCCGATATGCTCCAAGTCAGTGATTGACATGTCCAGAACCTGGCAGAGAATTGACGAGGAGATAGAAGCAACCGTTATGCCTGAGGATTACCGGTATAAGAAGGTATGGATCCTTTGCAATGACTGCAATGACACTACCGAAGTCTACTTCCACATAATTGGGCAGAAATGCAACCACTGCAACTCATACAACACACGCACGATTGCCCCTCCAGTTCTTCCTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000731 GO:0002039 GO:0002831 GO:0002832 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006281 GO:0006301 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008134 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016604 GO:0016607 GO:0016740 GO:0018130 GO:0019222 GO:0019438 GO:0019538 GO:0019787 GO:0019941 GO:0019985 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031347 GO:0031348 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032101 GO:0032102 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0042176 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0043900 GO:0043901 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045732 GO:0045862 GO:0046483 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051716 GO:0051865 GO:0060255 GO:0061136 GO:0065007 GO:0070013 GO:0070647 GO:0070987 GO:0071704 GO:0071897 GO:0080090 GO:0080134 GO:0090304 GO:0140096 GO:1900424 GO:1900425 GO:1901360 GO:1901362 GO:1901483 GO:1901485 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901800 GO:1902494 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:1990234 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
ko04115 ko04120 ko05162 map04115 map04120 map05162
Pfam Domains
Protein Families
zf-C3HC4 zf-CHY zf-RING_2 zf-RING_UBOX zinc_ribbon_6

Protein Analysis

268

Amino Acids

31.31

Weight (kDa)

6.88

Isoelectric Point (pI)

45.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CHY PF05495 18 - 91 1.1e-19 CHY zinc finger
zf-RING_2 PF13639 152 - 195 1.9e-07 Ring finger domain
zf-RING_5 PF14634 152 - 197 2.2e-06 zinc-RING finger domain
zf-C3HC4 PF00097 153 - 195 1.4e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 153 - 195 1.5e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 153 - 193 1.7e-06 RING-type zinc-finger
zinc_ribbon_6 PF14599 200 - 258 5.4e-27 Zinc-ribbon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 615
AccI GTMKAC 1 cut(s) 720
AccII CGCG 1 cut(s) 534
AciI CCGC 4 cut(s) 115, 252, 516, 532
AclWI GGATC 4 cut(s) 97, 350, 679, 692
AcsI RAATTY 2 cut(s) 290, 368
AfaI GTAC 1 cut(s) 244
AfiI CCNNNNNNNGG 2 cut(s) 162, 615
AflIII ACRYGT 1 cut(s) 603
AgeI ACCGGT 1 cut(s) 666
AgsI TTSAA 1 cut(s) 405
AjnI CCWGG 1 cut(s) 614
AleI CACNNNNGTG 1 cut(s) 429
AluBI AGCT 2 cut(s) 86, 172
AluI AGCT 2 cut(s) 86, 172
Alw21I GWGCWC 1 cut(s) 88
AlwI GGATC 4 cut(s) 97, 350, 679, 692
AlwNI CAGNNNCTG 1 cut(s) 615
ApeKI GCWGC 3 cut(s) 93, 223, 556
ApoI RAATTY 2 cut(s) 290, 368
AsiGI ACCGGT 1 cut(s) 666
Asp700I GAANNNNTTC 1 cut(s) 321
AxyI CCTNAGG 1 cut(s) 657
BaeGI GKGCMC 1 cut(s) 522
BamHI GGATCC 1 cut(s) 684
BanII GRGCYC 1 cut(s) 88
BarI GAAGNNNNNNTAC 2 cut(s) 29, 61
BauI CACGAG 1 cut(s) 136
Bbv12I GWGCWC 1 cut(s) 88
BbvI GCAGC 3 cut(s) 80, 235, 568
BccI CCATC 2 cut(s) 34, 114
BcgI CGANNNNNNTGC 2 cut(s) 72, 106
BciT130I CCWGG 1 cut(s) 616
BfmI CTRYAG 1 cut(s) 346
BisI GCNGC 5 cut(s) 94, 115, 224, 253, 557
BlpI GCTNAGC 1 cut(s) 10
BlsI GCNGC 5 cut(s) 95, 116, 225, 254, 558
Bme1390I CCNGG 1 cut(s) 616
BmiI GGNNCC 1 cut(s) 686
BmrFI CCNGG 1 cut(s) 616
BmsI GCATC 4 cut(s) 41, 62, 264, 543
BpmI CTGGAG 1 cut(s) 771
Bpu1102I GCTNAGC 1 cut(s) 10
BsaAI YACGTR 1 cut(s) 416
BsaWI WCCGGW 1 cut(s) 666
Bsc4I CCNNNNNNNGG 2 cut(s) 162, 615
Bse118I RCCGGY 2 cut(s) 226, 666
Bse1I ACTGG 1 cut(s) 788
Bse21I CCTNAGG 1 cut(s) 657
Bse3DI GCAATG 2 cut(s) 700, 709
BseBI CCWGG 1 cut(s) 616
BseGI GGATG 2 cut(s) 56, 106
BseLI CCNNNNNNNGG 2 cut(s) 162, 615
BseMI GCAATG 2 cut(s) 700, 709
BseMII CTCAG 2 cut(s) 24, 648
BseNI ACTGG 1 cut(s) 788
BseRI GAGGAG 1 cut(s) 647
BseSI GKGCMC 1 cut(s) 522
BseXI GCAGC 3 cut(s) 80, 235, 568
Bsh1236I CGCG 1 cut(s) 534
BshTI ACCGGT 1 cut(s) 666
BsiHKAI GWGCWC 1 cut(s) 88
BsiSI CCGG 2 cut(s) 227, 667
BslI CCNNNNNNNGG 2 cut(s) 162, 615
Bsp1286I GDGCHC 2 cut(s) 88, 522
Bsp1407I TGTACA 1 cut(s) 242
Bsp143I GATC 4 cut(s) 102, 160, 342, 684
Bsp1720I GCTNAGC 1 cut(s) 10
BspACI CCGC 4 cut(s) 115, 252, 516, 532
BspCNI CTCAG 2 cut(s) 23, 649
BspFNI CGCG 1 cut(s) 534
BspLI GGNNCC 1 cut(s) 686
BspMAI CTGCAG 1 cut(s) 350
BspPI GGATC 4 cut(s) 97, 350, 679, 692
BsrDI GCAATG 2 cut(s) 700, 709
BsrFI RCCGGY 2 cut(s) 226, 666
BsrGI TGTACA 1 cut(s) 242
BsrI ACTGG 1 cut(s) 788
BssAI RCCGGY 2 cut(s) 226, 666
BssMI GATC 4 cut(s) 102, 160, 342, 684
BssSI CACGAG 1 cut(s) 136
Bst2BI CACGAG 1 cut(s) 136
Bst2UI CCWGG 1 cut(s) 616
Bst4CI ACNGT 3 cut(s) 321, 505, 649
BstAPI GCANNNNNTGC 3 cut(s) 526, 699, 753
BstAUI TGTACA 1 cut(s) 242
BstBAI YACGTR 1 cut(s) 416
BstC8I GCNNGC 2 cut(s) 56, 518
BstDEI CTNAG 2 cut(s) 10, 657
BstF5I GGATG 2 cut(s) 56, 106
BstFNI CGCG 1 cut(s) 534
BstKTI GATC 4 cut(s) 105, 163, 345, 687
BstMBI GATC 4 cut(s) 102, 160, 342, 684
BstMWI GCNNNNNNNGC 4 cut(s) 147, 526, 699, 753
BstNI CCWGG 1 cut(s) 616
BstNSI RCATGY 2 cut(s) 145, 607
BstSCI CCNGG 1 cut(s) 614
BstSFI CTRYAG 1 cut(s) 346
BstSLI GKGCMC 1 cut(s) 522
BstUI CGCG 1 cut(s) 534
BstV1I GCAGC 3 cut(s) 80, 235, 568
BstX2I RGATCY 3 cut(s) 102, 342, 684
BstXI CCANNNNNNTGG 1 cut(s) 734
BstYI RGATCY 3 cut(s) 102, 342, 684
Bsu36I CCTNAGG 1 cut(s) 657
BtsCI GGATG 2 cut(s) 56, 106
BtsI GCAGTG 3 cut(s) 376, 454, 751
BtsIMutI CAGTG 4 cut(s) 376, 454, 600, 751
Cac8I GCNNGC 2 cut(s) 56, 518
CaiI CAGNNNCTG 1 cut(s) 615
Cfr10I RCCGGY 2 cut(s) 226, 666
CseI GACGC 1 cut(s) 437
Csp6I GTAC 1 cut(s) 243
CspAI ACCGGT 1 cut(s) 666
CspCI CAANNNNNGTGG 2 cut(s) 370, 405
CviAII CATG 4 cut(s) 142, 167, 445, 604
CviJI RGCY 6 cut(s) 9, 14, 86, 134, 172, 226
CviKI_1 RGCY 6 cut(s) 9, 14, 86, 134, 172, 226
CviQI GTAC 1 cut(s) 243
DdeI CTNAG 2 cut(s) 10, 657
DpnI GATC 4 cut(s) 104, 162, 344, 686
DpnII GATC 4 cut(s) 102, 160, 342, 684
Ecl136II GAGCTC 1 cut(s) 86
Eco24I GRGCYC 1 cut(s) 88
Eco53kI GAGCTC 1 cut(s) 86
Eco81I CCTNAGG 1 cut(s) 657
EcoICRI GAGCTC 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 614
EcoT38I GRGCYC 1 cut(s) 88
FaeI CATG 4 cut(s) 145, 170, 448, 607
FatI CATG 4 cut(s) 141, 166, 444, 603
FauI CCCGC 1 cut(s) 509
FblI GTMKAC 1 cut(s) 720
Fnu4HI GCNGC 5 cut(s) 94, 115, 224, 253, 557
FokI GGATG 2 cut(s) 63, 93
FriOI GRGCYC 1 cut(s) 88
Fsp4HI GCNGC 5 cut(s) 94, 115, 224, 253, 557
GluI GCNGC 5 cut(s) 94, 115, 224, 253, 557
GsuI CTGGAG 1 cut(s) 771
HapII CCGG 2 cut(s) 227, 667
HgaI GACGC 1 cut(s) 437
Hin1II CATG 4 cut(s) 145, 170, 448, 607
HinfI GANTC 3 cut(s) 79, 351, 485
HpaII CCGG 2 cut(s) 227, 667
Hpy166II GTNNAC 1 cut(s) 721
Hpy188I TCNGA 2 cut(s) 492, 579
Hpy188III TCNNGA 3 cut(s) 26, 362, 609
Hpy8I GTNNAC 1 cut(s) 721
HpyAV CCTTC 1 cut(s) 670
HpyCH4III ACNGT 3 cut(s) 321, 505, 649
HpyCH4IV ACGT 2 cut(s) 22, 415
HpyF10VI GCNNNNNNNGC 4 cut(s) 147, 526, 699, 753
HpyF3I CTNAG 2 cut(s) 10, 657
HpySE526I ACGT 2 cut(s) 22, 415
Hsp92II CATG 4 cut(s) 145, 170, 448, 607
Kzo9I GATC 4 cut(s) 102, 160, 342, 684
LmnI GCTCC 2 cut(s) 91, 590
LpnPI CCDG 7 cut(s) 89, 240, 601, 622, 628, 669, 680
Lsp1109I GCAGC 3 cut(s) 80, 235, 568
LweI GCATC 4 cut(s) 41, 62, 264, 543
MaeII ACGT 2 cut(s) 22, 415
MaeIII GTNAC 8 cut(s) 44, 122, 212, 278, 362, 467, 539, 560
MalI GATC 4 cut(s) 104, 162, 344, 686
MboI GATC 4 cut(s) 102, 160, 342, 684
MboII GAAGA 4 cut(s) 49, 78, 81, 785
MflI RGATCY 3 cut(s) 102, 342, 684
MhlI GDGCHC 2 cut(s) 88, 522
MluCI AATT 5 cut(s) 290, 368, 405, 624, 732
MlyI GAGTC 1 cut(s) 479
MnlI CCTC 3 cut(s) 625, 652, 795
MroXI GAANNNNTTC 1 cut(s) 321
MslI CAYNNNNRTG 4 cut(s) 140, 260, 330, 429
MspI CCGG 2 cut(s) 227, 667
MspR9I CCNGG 1 cut(s) 616
MvaI CCWGG 1 cut(s) 616
MvnI CGCG 1 cut(s) 534
MwoI GCNNNNNNNGC 4 cut(s) 147, 526, 699, 753
NdeII GATC 4 cut(s) 102, 160, 342, 684
NlaIII CATG 4 cut(s) 145, 170, 448, 607
NlaIV GGNNCC 1 cut(s) 686
NmuCI GTSAC 5 cut(s) 122, 212, 278, 362, 560
NspI RCATGY 2 cut(s) 145, 607
OliI CACNNNNGTG 1 cut(s) 429
PciI ACATGT 1 cut(s) 603
PdmI GAANNNNTTC 1 cut(s) 321
PfeI GAWTC 2 cut(s) 79, 351
PflMI CCANNNNNTGG 1 cut(s) 615
PinAI ACCGGT 1 cut(s) 666
PkrI GCNGC 5 cut(s) 95, 116, 225, 254, 558
PleI GAGTC 1 cut(s) 479
PpsI GAGTC 1 cut(s) 479
Ppu21I YACGTR 1 cut(s) 416
PscI ACATGT 1 cut(s) 603
Psp124BI GAGCTC 1 cut(s) 88
Psp6I CCWGG 1 cut(s) 614
PspGI CCWGG 1 cut(s) 614
PspN4I GGNNCC 1 cut(s) 686
PstI CTGCAG 1 cut(s) 350
PstNI CAGNNNCTG 1 cut(s) 615
PsuI RGATCY 3 cut(s) 102, 342, 684
RsaI GTAC 1 cut(s) 244
RsaNI GTAC 1 cut(s) 243
RseI CAYNNNNRTG 4 cut(s) 140, 260, 330, 429
SacI GAGCTC 1 cut(s) 88
SatI GCNGC 5 cut(s) 94, 115, 224, 253, 557
Sau3AI GATC 4 cut(s) 102, 160, 342, 684
SchI GAGTC 1 cut(s) 479
ScrFI CCNGG 1 cut(s) 616
SduI GDGCHC 2 cut(s) 88, 522
SetI ASST 6 cut(s) 25, 88, 174, 418, 617, 681
SfaNI GCATC 4 cut(s) 41, 62, 264, 543
SfcI CTRYAG 1 cut(s) 346
SmiMI CAYNNNNRTG 4 cut(s) 140, 260, 330, 429
Sse9I AATT 5 cut(s) 290, 368, 405, 624, 732
SsiI CCGC 4 cut(s) 115, 252, 516, 532
SspI AATATT 1 cut(s) 569
SstI GAGCTC 1 cut(s) 88
StyD4I CCNGG 1 cut(s) 614
TaaI ACNGT 3 cut(s) 321, 505, 649
TaiI ACGT 2 cut(s) 25, 418
TaqI TCGA 1 cut(s) 82
TasI AATT 5 cut(s) 290, 368, 405, 624, 732
TatI WGTACW 1 cut(s) 242
TauI GCSGC 2 cut(s) 117, 255
TfiI GAWTC 2 cut(s) 79, 351
TscAI CASTG 4 cut(s) 383, 461, 600, 758
TseFI GTSAC 5 cut(s) 122, 212, 278, 362, 560
TseI GCWGC 3 cut(s) 93, 223, 556
Tsp45I GTSAC 5 cut(s) 122, 212, 278, 362, 560
TspDTI ATGAA 3 cut(s) 314, 524, 561
TspGWI ACGGA 3 cut(s) 63, 78, 99
TspRI CASTG 4 cut(s) 383, 461, 600, 758
Van91I CCANNNNNTGG 1 cut(s) 615
XapI RAATTY 2 cut(s) 290, 368
XceI RCATGY 2 cut(s) 145, 607
XmiI GTMKAC 1 cut(s) 720
XmnI GAANNNNTTC 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.