pycom16g14590

RING finger and CHY zinc finger domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009916
Physical Location & Seq
Forward (+)
10673607 .. 10675977
2371 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g14590.1

Sequence Viewer

Length: 828 bp
ATGGAAGGCTCAGCCAATGAACGTCTTGATTTTGGGAAGATGGGTTACGGATGCAAGCATTACAGAAGAAGATGCCAGATTCGAGCTCCGTGCTGCAACGAGATGTATCCGTGCCGCCATTGTCACAACGAAGCCACGAGCATGTTGAGCAACCCCTTTGATCGGCATGAGCTTGTTCGCTACGATGTGAAACAAGTTGTCTGTTCAGTTTGTGACACAGAGCAGCCGGTTTGTTTTCCTACTTTGTTGGTTGTTGCAAAAGTTTGTACAAACTGCGGCGTCAGTATGGGGGAATACTTCTGTGACATTTGCAAATTCTATGATGATGATACCACGAAAGAACAATTTCATTGTAATGATTGTGGGATCTGCAGAATCGGCGGTCGTGACAAATTCTATCACTGCAAGAAATGTGGGTCTTGCTATTCAATTGGCTTACGTGATAATCACTTGTGTGTGGAGAACTCCATGCGGCATCACTGCCCTATTTGTTACGAGTTTCTTTTTGACTCTCTGAAGGACACTACTGTAATGAAATGCGGGCACACAATGCACTGCGAATGTTACAATGAAATGATGAAGCGTGACAAATATTGTTGTCCGATATGCTCCAAGTCAGTGATTGACATGTCCAGAACCTGGAAGAGAATCGACGAGGAGATAGAAGCAACCGTTATGCCTGAGGATTACCGGTATAAGAAGGTATGGATCCTTTGCAATGACTGCAACGACACTACCGAAGTCTACTTCCACATAATTGGGCAGAAATGCAACCACTGCAACTCATACAACACACGCACGATTGCCCCTCCAGTTCTTCCTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000731 GO:0002039 GO:0002831 GO:0002832 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006281 GO:0006301 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008134 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016604 GO:0016607 GO:0016740 GO:0018130 GO:0019222 GO:0019438 GO:0019538 GO:0019787 GO:0019941 GO:0019985 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031347 GO:0031348 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032101 GO:0032102 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0042176 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0043900 GO:0043901 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045732 GO:0045862 GO:0046483 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051716 GO:0051865 GO:0060255 GO:0061136 GO:0065007 GO:0070013 GO:0070647 GO:0070987 GO:0071704 GO:0071897 GO:0080090 GO:0080134 GO:0090304 GO:0140096 GO:1900424 GO:1900425 GO:1901360 GO:1901362 GO:1901483 GO:1901485 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901800 GO:1902494 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:1990234 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
ko04115 ko04120 ko05162 map04115 map04120 map05162
Pfam Domains
Protein Families
zf-C3HC4 zf-CHY zf-RING_2 zf-RING_UBOX zinc_ribbon_6

Protein Analysis

276

Amino Acids

32.08

Weight (kDa)

6.85

Isoelectric Point (pI)

50.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CHY PF05495 18 - 99 1.2e-17 CHY zinc finger
zf-RING_2 PF13639 160 - 203 2.5e-06 Ring finger domain
zinc_ribbon_6 PF14599 208 - 266 4.9e-27 Zinc-ribbon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 639
AccI GTMKAC 1 cut(s) 744
AciI CCGC 5 cut(s) 115, 276, 381, 472, 540
AclWI GGATC 3 cut(s) 374, 703, 716
AcsI RAATTY 2 cut(s) 314, 392
AcuI CTGAAG 1 cut(s) 536
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 1 cut(s) 268
AfiI CCNNNNNNNGG 2 cut(s) 162, 639
AflIII ACRYGT 1 cut(s) 627
AgeI ACCGGT 1 cut(s) 690
AgsI TTSAA 1 cut(s) 429
AjnI CCWGG 1 cut(s) 638
AleI CACNNNNGTG 1 cut(s) 453
AluBI AGCT 2 cut(s) 86, 172
AluI AGCT 2 cut(s) 86, 172
Alw21I GWGCWC 1 cut(s) 88
AlwI GGATC 3 cut(s) 374, 703, 716
AlwNI CAGNNNCTG 1 cut(s) 639
ApeKI GCWGC 2 cut(s) 93, 223
ApoI RAATTY 2 cut(s) 314, 392
AsiGI ACCGGT 1 cut(s) 690
Asp700I GAANNNNTTC 1 cut(s) 345
AxyI CCTNAGG 1 cut(s) 681
BaeGI GKGCMC 1 cut(s) 546
BamHI GGATCC 1 cut(s) 708
BanII GRGCYC 1 cut(s) 88
BarI GAAGNNNNNNTAC 2 cut(s) 29, 61
BauI CACGAG 1 cut(s) 136
Bbv12I GWGCWC 1 cut(s) 88
BbvI GCAGC 2 cut(s) 80, 235
BccI CCATC 1 cut(s) 34
BcgI CGANNNNNNTGC 2 cut(s) 72, 106
BciT130I CCWGG 1 cut(s) 640
BciVI GTATCC 1 cut(s) 117
BfmI CTRYAG 1 cut(s) 370
BfuI GTATCC 1 cut(s) 117
BisI GCNGC 5 cut(s) 94, 115, 224, 277, 473
BlpI GCTNAGC 1 cut(s) 10
BlsI GCNGC 5 cut(s) 95, 116, 225, 278, 474
Bme1390I CCNGG 1 cut(s) 640
BmiI GGNNCC 1 cut(s) 710
BmrFI CCNGG 1 cut(s) 640
BmsI GCATC 3 cut(s) 41, 62, 484
BpmI CTGGAG 1 cut(s) 795
Bpu1102I GCTNAGC 1 cut(s) 10
BsaAI YACGTR 1 cut(s) 440
BsaHI GRCGYC 1 cut(s) 279
BsaWI WCCGGW 1 cut(s) 690
Bsc4I CCNNNNNNNGG 2 cut(s) 162, 639
Bse118I RCCGGY 2 cut(s) 226, 690
Bse1I ACTGG 1 cut(s) 812
Bse21I CCTNAGG 1 cut(s) 681
Bse3DI GCAATG 1 cut(s) 724
BseBI CCWGG 1 cut(s) 640
BseGI GGATG 1 cut(s) 56
BseLI CCNNNNNNNGG 2 cut(s) 162, 639
BseMI GCAATG 1 cut(s) 724
BseMII CTCAG 2 cut(s) 24, 672
BseNI ACTGG 1 cut(s) 812
BseRI GAGGAG 1 cut(s) 671
BseSI GKGCMC 1 cut(s) 546
BseXI GCAGC 2 cut(s) 80, 235
Bsh1285I CGRYCG 1 cut(s) 385
BshTI ACCGGT 1 cut(s) 690
BsiEI CGRYCG 1 cut(s) 385
BsiHKAI GWGCWC 1 cut(s) 88
BsiSI CCGG 2 cut(s) 227, 691
BslI CCNNNNNNNGG 2 cut(s) 162, 639
Bsp1286I GDGCHC 2 cut(s) 88, 546
Bsp1407I TGTACA 1 cut(s) 266
Bsp143I GATC 3 cut(s) 160, 366, 708
Bsp1720I GCTNAGC 1 cut(s) 10
BspACI CCGC 5 cut(s) 115, 276, 381, 472, 540
BspCNI CTCAG 2 cut(s) 23, 673
BspLI GGNNCC 1 cut(s) 710
BspMAI CTGCAG 1 cut(s) 374
BspPI GGATC 3 cut(s) 374, 703, 716
BsrDI GCAATG 1 cut(s) 724
BsrFI RCCGGY 2 cut(s) 226, 690
BsrGI TGTACA 1 cut(s) 266
BsrI ACTGG 1 cut(s) 812
BssAI RCCGGY 2 cut(s) 226, 690
BssMI GATC 3 cut(s) 160, 366, 708
BssNI GRCGYC 1 cut(s) 279
BssSI CACGAG 1 cut(s) 136
Bst2BI CACGAG 1 cut(s) 136
Bst2UI CCWGG 1 cut(s) 640
Bst4CI ACNGT 2 cut(s) 529, 673
Bst6I CTCTTC 1 cut(s) 638
BstACI GRCGYC 1 cut(s) 279
BstAPI GCANNNNNTGC 3 cut(s) 550, 723, 777
BstAUI TGTACA 1 cut(s) 266
BstBAI YACGTR 1 cut(s) 440
BstC8I GCNNGC 2 cut(s) 56, 542
BstDEI CTNAG 2 cut(s) 10, 681
BstF5I GGATG 1 cut(s) 56
BstKTI GATC 3 cut(s) 163, 369, 711
BstMBI GATC 3 cut(s) 160, 366, 708
BstMCI CGRYCG 1 cut(s) 385
BstMWI GCNNNNNNNGC 5 cut(s) 147, 378, 550, 723, 777
BstNI CCWGG 1 cut(s) 640
BstNSI RCATGY 2 cut(s) 145, 631
BstSCI CCNGG 1 cut(s) 638
BstSFI CTRYAG 1 cut(s) 370
BstSLI GKGCMC 1 cut(s) 546
BstV1I GCAGC 2 cut(s) 80, 235
BstX2I RGATCY 2 cut(s) 366, 708
BstXI CCANNNNNNTGG 1 cut(s) 758
BstYI RGATCY 2 cut(s) 366, 708
Bsu36I CCTNAGG 1 cut(s) 681
BsuI GTATCC 1 cut(s) 117
BtsCI GGATG 1 cut(s) 56
BtsI GCAGTG 4 cut(s) 400, 478, 553, 775
BtsIMutI CAGTG 5 cut(s) 400, 478, 553, 624, 775
Cac8I GCNNGC 2 cut(s) 56, 542
CaiI CAGNNNCTG 1 cut(s) 639
Cfr10I RCCGGY 2 cut(s) 226, 690
CseI GACGC 1 cut(s) 268
Csp6I GTAC 1 cut(s) 267
CspAI ACCGGT 1 cut(s) 690
CspCI CAANNNNNGTGG 2 cut(s) 394, 429
CviAII CATG 4 cut(s) 142, 167, 469, 628
CviJI RGCY 7 cut(s) 9, 14, 86, 134, 172, 226, 435
CviKI_1 RGCY 7 cut(s) 9, 14, 86, 134, 172, 226, 435
CviQI GTAC 1 cut(s) 267
DdeI CTNAG 2 cut(s) 10, 681
DpnI GATC 3 cut(s) 162, 368, 710
DpnII GATC 3 cut(s) 160, 366, 708
Eam1104I CTCTTC 1 cut(s) 638
EarI CTCTTC 1 cut(s) 638
Ecl136II GAGCTC 1 cut(s) 86
Eco24I GRGCYC 1 cut(s) 88
Eco53kI GAGCTC 1 cut(s) 86
Eco57I CTGAAG 1 cut(s) 536
Eco81I CCTNAGG 1 cut(s) 681
EcoICRI GAGCTC 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 638
EcoT38I GRGCYC 1 cut(s) 88
FaeI CATG 4 cut(s) 145, 170, 472, 631
FatI CATG 4 cut(s) 141, 166, 468, 627
FauI CCCGC 1 cut(s) 533
FblI GTMKAC 1 cut(s) 744
Fnu4HI GCNGC 5 cut(s) 94, 115, 224, 277, 473
FokI GGATG 1 cut(s) 63
FriOI GRGCYC 1 cut(s) 88
Fsp4HI GCNGC 5 cut(s) 94, 115, 224, 277, 473
GluI GCNGC 5 cut(s) 94, 115, 224, 277, 473
GsuI CTGGAG 1 cut(s) 795
HapII CCGG 2 cut(s) 227, 691
HgaI GACGC 1 cut(s) 268
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 4 cut(s) 145, 170, 472, 631
HinfI GANTC 4 cut(s) 79, 375, 509, 648
HpaII CCGG 2 cut(s) 227, 691
Hpy166II GTNNAC 1 cut(s) 745
Hpy188I TCNGA 2 cut(s) 516, 603
Hpy188III TCNNGA 3 cut(s) 26, 386, 633
Hpy8I GTNNAC 1 cut(s) 745
Hpy99I CGWCG 1 cut(s) 656
HpyAV CCTTC 2 cut(s) 511, 694
HpyCH4III ACNGT 2 cut(s) 529, 673
HpyCH4IV ACGT 2 cut(s) 22, 439
HpyF10VI GCNNNNNNNGC 5 cut(s) 147, 378, 550, 723, 777
HpyF3I CTNAG 2 cut(s) 10, 681
HpySE526I ACGT 2 cut(s) 22, 439
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 4 cut(s) 145, 170, 472, 631
Kzo9I GATC 3 cut(s) 160, 366, 708
LmnI GCTCC 2 cut(s) 91, 614
LpnPI CCDG 7 cut(s) 89, 240, 625, 646, 652, 693, 704
Lsp1109I GCAGC 2 cut(s) 80, 235
LweI GCATC 3 cut(s) 41, 62, 484
MaeII ACGT 2 cut(s) 22, 439
MaeIII GTNAC 8 cut(s) 44, 122, 212, 302, 386, 491, 563, 584
MalI GATC 3 cut(s) 162, 368, 710
MboI GATC 3 cut(s) 160, 366, 708
MboII GAAGA 5 cut(s) 49, 78, 81, 655, 809
MfeI CAATTG 1 cut(s) 429
MflI RGATCY 2 cut(s) 366, 708
MhlI GDGCHC 2 cut(s) 88, 546
MluCI AATT 5 cut(s) 314, 344, 392, 429, 756
MlyI GAGTC 1 cut(s) 503
MnlI CCTC 3 cut(s) 649, 676, 819
MroXI GAANNNNTTC 1 cut(s) 345
MslI CAYNNNNRTG 3 cut(s) 140, 354, 453
MspI CCGG 2 cut(s) 227, 691
MspR9I CCNGG 1 cut(s) 640
MunI CAATTG 1 cut(s) 429
MvaI CCWGG 1 cut(s) 640
MwoI GCNNNNNNNGC 5 cut(s) 147, 378, 550, 723, 777
NdeII GATC 3 cut(s) 160, 366, 708
NlaIII CATG 4 cut(s) 145, 170, 472, 631
NlaIV GGNNCC 1 cut(s) 710
NmuCI GTSAC 5 cut(s) 122, 212, 302, 386, 584
NspI RCATGY 2 cut(s) 145, 631
OliI CACNNNNGTG 1 cut(s) 453
PciI ACATGT 1 cut(s) 627
PcsI WCGNNNNNNNCGW 1 cut(s) 735
PdmI GAANNNNTTC 1 cut(s) 345
PfeI GAWTC 3 cut(s) 79, 375, 648
PflMI CCANNNNNTGG 1 cut(s) 639
PinAI ACCGGT 1 cut(s) 690
PkrI GCNGC 5 cut(s) 95, 116, 225, 278, 474
PleI GAGTC 1 cut(s) 503
PpsI GAGTC 1 cut(s) 503
Ppu21I YACGTR 1 cut(s) 440
PscI ACATGT 1 cut(s) 627
Psp124BI GAGCTC 1 cut(s) 88
Psp6I CCWGG 1 cut(s) 638
PspGI CCWGG 1 cut(s) 638
PspN4I GGNNCC 1 cut(s) 710
PstI CTGCAG 1 cut(s) 374
PstNI CAGNNNCTG 1 cut(s) 639
PsuI RGATCY 2 cut(s) 366, 708
RsaI GTAC 1 cut(s) 268
RsaNI GTAC 1 cut(s) 267
RseI CAYNNNNRTG 3 cut(s) 140, 354, 453
SacI GAGCTC 1 cut(s) 88
SatI GCNGC 5 cut(s) 94, 115, 224, 277, 473
Sau3AI GATC 3 cut(s) 160, 366, 708
SchI GAGTC 1 cut(s) 503
ScrFI CCNGG 1 cut(s) 640
SduI GDGCHC 2 cut(s) 88, 546
SetI ASST 6 cut(s) 25, 88, 174, 442, 641, 705
SfaNI GCATC 3 cut(s) 41, 62, 484
SfcI CTRYAG 1 cut(s) 370
SmiMI CAYNNNNRTG 3 cut(s) 140, 354, 453
Sse9I AATT 5 cut(s) 314, 344, 392, 429, 756
SsiI CCGC 5 cut(s) 115, 276, 381, 472, 540
SspI AATATT 1 cut(s) 593
SstI GAGCTC 1 cut(s) 88
StyD4I CCNGG 1 cut(s) 638
TaaI ACNGT 2 cut(s) 529, 673
TaiI ACGT 2 cut(s) 25, 442
TaqI TCGA 2 cut(s) 82, 651
TasI AATT 5 cut(s) 314, 344, 392, 429, 756
TatI WGTACW 1 cut(s) 266
TauI GCSGC 3 cut(s) 117, 279, 475
TfiI GAWTC 3 cut(s) 79, 375, 648
TscAI CASTG 5 cut(s) 407, 485, 560, 624, 782
TseFI GTSAC 5 cut(s) 122, 212, 302, 386, 584
TseI GCWGC 2 cut(s) 93, 223
Tsp45I GTSAC 5 cut(s) 122, 212, 302, 386, 584
TspDTI ATGAA 5 cut(s) 33, 338, 548, 585, 593
TspGWI ACGGA 3 cut(s) 63, 78, 99
TspRI CASTG 5 cut(s) 407, 485, 560, 624, 782
Van91I CCANNNNNTGG 1 cut(s) 639
XapI RAATTY 2 cut(s) 314, 392
XceI RCATGY 2 cut(s) 145, 631
XmiI GTMKAC 1 cut(s) 744
XmnI GAANNNNTTC 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.