pycom13g14840

RING finger and CHY zinc finger domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009916
Physical Location & Seq
Forward (+)
10743128 .. 10747674
4547 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g14840.1

Sequence Viewer

Length: 885 bp
ATGCAGACCGTGATCGCAAACTGTAATGACATACAACCGGTTAGATTTTCTGCGTACGATCTAATAGATGAAACCTCAAAGACTACAAGTCTCAGTATGCAAACATTACAGAAGAAGATGCCAGATTCGAGCTCCGTGCTGCAACGAGATCGATCCTTGTCGCCATTGTCACAACGAAGCCACGGTATTGGTCACAGCATGTTGAGCAACCCCTTTGATCGCCATGAGCTCGTTCGCTACGATGTGAAACAAGTTGTTTGTTCGGTTTGTGACACAGAGCAGCCGGTTTGTGTTCTTACTTCTTACTATGTTGCACGAGTTTGTACAAACTGCGGCGTCAGTATGGGGGAATATTTCTGTGACATTTGCAAATTCTATGATGATGATACCACGAAAGAACAATTTCATTGTAATGATTGTGGGATCTGCAGAATCGGGGGTCGTGACAAATTTTATCACTGCAAGAAATGTGGTTCTTGCTATTCAAATGGCTTACGTGATAATCACTTGTGTGTGGAGAACTCAATGCAGCATCACTGCCCTATTTGTTACGAGTTCCTTTTTGACTCACTGAAAGACACTACTGTAATGAAATGCGGGCACACAATGCACTGCGAATGTTATAATGAAATGATGAAGCGTGACAAATATTGTTGTCCGATATGCTCCAAGTCGGTGATTGACATGTCCAAAACCTGGAAGAGAATAGATGAGGAGATAGAAGCAACTGTTATGCCTGAGGATTACCGGTATAAGAAGGTATGGATCCTGTGCAATGACTGCAACGACACTACCGAAGTCTACTTCCACATTATTGGGCAGAAATGCAACCACTGCAACTCATACAACACACGCACGATTGCCCCACCAGTTCTTCCTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000731 GO:0002039 GO:0002831 GO:0002832 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006281 GO:0006301 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008134 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016604 GO:0016607 GO:0016740 GO:0018130 GO:0019222 GO:0019438 GO:0019538 GO:0019787 GO:0019941 GO:0019985 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031347 GO:0031348 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032101 GO:0032102 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0042176 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0043900 GO:0043901 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045732 GO:0045862 GO:0046483 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051716 GO:0051865 GO:0060255 GO:0061136 GO:0065007 GO:0070013 GO:0070647 GO:0070987 GO:0071704 GO:0071897 GO:0080090 GO:0080134 GO:0090304 GO:0140096 GO:1900424 GO:1900425 GO:1901360 GO:1901362 GO:1901483 GO:1901485 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901800 GO:1902494 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:1990234 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
ko04115 ko04120 ko05162 map04115 map04120 map05162
Pfam Domains
Protein Families
zf-C3HC4 zf-CHY zf-RING_2 zf-RING_UBOX zinc_ribbon_6

Protein Analysis

295

Amino Acids

33.93

Weight (kDa)

6.3

Isoelectric Point (pI)

49.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 179 - 222 5.7e-06 Ring finger domain
zinc_ribbon_6 PF14599 227 - 285 5.1e-27 Zinc-ribbon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 624
AccB7I CCANNNNNTGG 1 cut(s) 696
AccI GTMKAC 1 cut(s) 801
AciI CCGC 2 cut(s) 333, 597
AclWI GGATC 4 cut(s) 147, 431, 760, 773
AcsI RAATTY 2 cut(s) 371, 449
AcyI GRCGYC 1 cut(s) 336
AfaI GTAC 2 cut(s) 56, 325
AfiI CCNNNNNNNGG 1 cut(s) 696
AflIII ACRYGT 1 cut(s) 684
AgeI ACCGGT 2 cut(s) 37, 747
AgsI TTSAA 1 cut(s) 486
AhdI GACNNNNNGTC 1 cut(s) 87
AjnI CCWGG 1 cut(s) 695
AleI CACNNNNGTG 1 cut(s) 510
AluBI AGCT 2 cut(s) 132, 229
AluI AGCT 2 cut(s) 132, 229
Alw21I GWGCWC 2 cut(s) 134, 231
Alw26I GTCTC 1 cut(s) 95
AlwI GGATC 4 cut(s) 147, 431, 760, 773
ApeKI GCWGC 3 cut(s) 139, 280, 529
ApoI RAATTY 2 cut(s) 371, 449
AsiGI ACCGGT 2 cut(s) 37, 747
Asp700I GAANNNNTTC 1 cut(s) 402
AsuHPI GGTGA 1 cut(s) 688
AxyI CCTNAGG 1 cut(s) 738
BaeGI GKGCMC 1 cut(s) 603
BamHI GGATCC 1 cut(s) 765
BanII GRGCYC 2 cut(s) 134, 231
BauI CACGAG 1 cut(s) 315
Bbv12I GWGCWC 2 cut(s) 134, 231
BbvI GCAGC 3 cut(s) 126, 292, 541
BcgI CGANNNNNNTGC 4 cut(s) 118, 131, 152, 165
BciT130I CCWGG 1 cut(s) 697
BcoDI GTCTC 1 cut(s) 95
BfmI CTRYAG 1 cut(s) 427
BisI GCNGC 4 cut(s) 140, 281, 334, 530
BlsI GCNGC 4 cut(s) 141, 282, 335, 531
Bme1390I CCNGG 1 cut(s) 697
BmeRI GACNNNNNGTC 1 cut(s) 87
BmiI GGNNCC 1 cut(s) 767
BmrFI CCNGG 1 cut(s) 697
BmsI GCATC 2 cut(s) 108, 541
Bsa29I ATCGAT 1 cut(s) 151
BsaAI YACGTR 1 cut(s) 497
BsaHI GRCGYC 1 cut(s) 336
BsaJI CCNNGG 1 cut(s) 181
BsaWI WCCGGW 2 cut(s) 37, 747
Bsc4I CCNNNNNNNGG 1 cut(s) 696
Bse118I RCCGGY 3 cut(s) 37, 283, 747
Bse1I ACTGG 1 cut(s) 869
Bse21I CCTNAGG 1 cut(s) 738
Bse3DI GCAATG 1 cut(s) 781
BseBI CCWGG 1 cut(s) 697
BseCI ATCGAT 1 cut(s) 151
BseDI CCNNGG 1 cut(s) 181
BseLI CCNNNNNNNGG 1 cut(s) 696
BseMI GCAATG 1 cut(s) 781
BseMII CTCAG 2 cut(s) 106, 729
BseNI ACTGG 1 cut(s) 869
BseRI GAGGAG 1 cut(s) 728
BseSI GKGCMC 1 cut(s) 603
BseXI GCAGC 3 cut(s) 126, 292, 541
BshTI ACCGGT 2 cut(s) 37, 747
BshVI ATCGAT 1 cut(s) 151
BsiHKAI GWGCWC 2 cut(s) 134, 231
BsiSI CCGG 3 cut(s) 38, 284, 748
BsiWI CGTACG 1 cut(s) 54
BslI CCNNNNNNNGG 1 cut(s) 696
BsmAI GTCTC 1 cut(s) 95
Bsp1286I GDGCHC 3 cut(s) 134, 231, 603
Bsp1407I TGTACA 1 cut(s) 323
Bsp143I GATC 7 cut(s) 12, 58, 148, 152, 217, 423, 765
BspACI CCGC 2 cut(s) 333, 597
BspCNI CTCAG 2 cut(s) 105, 730
BspDI ATCGAT 1 cut(s) 151
BspLI GGNNCC 1 cut(s) 767
BspMAI CTGCAG 1 cut(s) 431
BspPI GGATC 4 cut(s) 147, 431, 760, 773
BsrDI GCAATG 1 cut(s) 781
BsrFI RCCGGY 3 cut(s) 37, 283, 747
BsrGI TGTACA 1 cut(s) 323
BsrI ACTGG 1 cut(s) 869
BssAI RCCGGY 3 cut(s) 37, 283, 747
BssECI CCNNGG 1 cut(s) 181
BssMI GATC 7 cut(s) 12, 58, 148, 152, 217, 423, 765
BssNI GRCGYC 1 cut(s) 336
BssSI CACGAG 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 315
Bst2UI CCWGG 1 cut(s) 697
Bst4CI ACNGT 5 cut(s) 10, 23, 185, 586, 730
Bst6I CTCTTC 1 cut(s) 695
BstACI GRCGYC 1 cut(s) 336
BstAPI GCANNNNNTGC 3 cut(s) 607, 780, 834
BstAUI TGTACA 1 cut(s) 323
BstBAI YACGTR 1 cut(s) 497
BstC8I GCNNGC 1 cut(s) 599
BstDEI CTNAG 2 cut(s) 92, 738
BstDSI CCRYGG 1 cut(s) 181
BstKTI GATC 7 cut(s) 15, 61, 151, 155, 220, 426, 768
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 7 cut(s) 12, 58, 148, 152, 217, 423, 765
BstMWI GCNNNNNNNGC 4 cut(s) 204, 607, 780, 834
BstNI CCWGG 1 cut(s) 697
BstNSI RCATGY 2 cut(s) 202, 688
BstSCI CCNGG 1 cut(s) 695
BstSFI CTRYAG 1 cut(s) 427
BstSLI GKGCMC 1 cut(s) 603
BstV1I GCAGC 3 cut(s) 126, 292, 541
BstX2I RGATCY 2 cut(s) 423, 765
BstXI CCANNNNNNTGG 2 cut(s) 188, 815
BstYI RGATCY 2 cut(s) 423, 765
Bsu15I ATCGAT 1 cut(s) 151
Bsu36I CCTNAGG 1 cut(s) 738
BsuTUI ATCGAT 1 cut(s) 151
BtgI CCRYGG 1 cut(s) 181
BtsI GCAGTG 4 cut(s) 457, 535, 610, 832
BtsIMutI CAGTG 5 cut(s) 457, 535, 569, 610, 832
Cac8I GCNNGC 1 cut(s) 599
Cfr10I RCCGGY 3 cut(s) 37, 283, 747
ClaI ATCGAT 1 cut(s) 151
CseI GACGC 1 cut(s) 325
Csp6I GTAC 2 cut(s) 55, 324
CspAI ACCGGT 2 cut(s) 37, 747
CspCI CAANNNNNGTGG 2 cut(s) 451, 486
CviAII CATG 3 cut(s) 199, 224, 685
CviJI RGCY 5 cut(s) 132, 180, 229, 283, 492
CviKI_1 RGCY 5 cut(s) 132, 180, 229, 283, 492
CviQI GTAC 2 cut(s) 55, 324
DdeI CTNAG 2 cut(s) 92, 738
DpnI GATC 7 cut(s) 14, 60, 150, 154, 219, 425, 767
DpnII GATC 7 cut(s) 12, 58, 148, 152, 217, 423, 765
DriI GACNNNNNGTC 1 cut(s) 87
Eam1104I CTCTTC 1 cut(s) 695
Eam1105I GACNNNNNGTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 695
Ecl136II GAGCTC 2 cut(s) 132, 229
Eco24I GRGCYC 2 cut(s) 134, 231
Eco53kI GAGCTC 2 cut(s) 132, 229
Eco81I CCTNAGG 1 cut(s) 738
EcoICRI GAGCTC 2 cut(s) 132, 229
EcoRII CCWGG 1 cut(s) 695
EcoT38I GRGCYC 2 cut(s) 134, 231
FaeI CATG 3 cut(s) 202, 227, 688
FatI CATG 3 cut(s) 198, 223, 684
FauI CCCGC 1 cut(s) 590
FblI GTMKAC 1 cut(s) 801
Fnu4HI GCNGC 4 cut(s) 140, 281, 334, 530
FriOI GRGCYC 2 cut(s) 134, 231
Fsp4HI GCNGC 4 cut(s) 140, 281, 334, 530
GluI GCNGC 4 cut(s) 140, 281, 334, 530
HapII CCGG 3 cut(s) 38, 284, 748
HgaI GACGC 1 cut(s) 325
Hin1I GRCGYC 1 cut(s) 336
Hin1II CATG 3 cut(s) 202, 227, 688
HinfI GANTC 3 cut(s) 125, 432, 566
HpaII CCGG 3 cut(s) 38, 284, 748
HphI GGTGA 1 cut(s) 688
Hpy166II GTNNAC 1 cut(s) 802
Hpy188I TCNGA 1 cut(s) 660
Hpy188III TCNNGA 1 cut(s) 443
Hpy8I GTNNAC 1 cut(s) 802
HpyAV CCTTC 1 cut(s) 751
HpyCH4III ACNGT 5 cut(s) 10, 23, 185, 586, 730
HpyCH4IV ACGT 1 cut(s) 496
HpyF10VI GCNNNNNNNGC 4 cut(s) 204, 607, 780, 834
HpyF3I CTNAG 2 cut(s) 92, 738
HpySE526I ACGT 1 cut(s) 496
Hsp92I GRCGYC 1 cut(s) 336
Hsp92II CATG 3 cut(s) 202, 227, 688
Kzo9I GATC 7 cut(s) 12, 58, 148, 152, 217, 423, 765
LmnI GCTCC 2 cut(s) 137, 671
LpnPI CCDG 8 cut(s) 51, 135, 297, 682, 709, 750, 761, 782
Lsp1109I GCAGC 3 cut(s) 126, 292, 541
LweI GCATC 2 cut(s) 108, 541
MaeII ACGT 1 cut(s) 496
MaeIII GTNAC 7 cut(s) 168, 191, 269, 359, 443, 548, 641
MalI GATC 7 cut(s) 14, 60, 150, 154, 219, 425, 767
MboI GATC 7 cut(s) 12, 58, 148, 152, 217, 423, 765
MboII GAAGA 4 cut(s) 124, 127, 712, 866
MflI RGATCY 2 cut(s) 423, 765
MhlI GDGCHC 3 cut(s) 134, 231, 603
MluCI AATT 3 cut(s) 371, 401, 449
MlyI GAGTC 1 cut(s) 560
MnlI CCTC 3 cut(s) 85, 706, 733
MroXI GAANNNNTTC 1 cut(s) 402
MslI CAYNNNNRTG 2 cut(s) 411, 510
MspI CCGG 3 cut(s) 38, 284, 748
MspR9I CCNGG 1 cut(s) 697
MvaI CCWGG 1 cut(s) 697
MwoI GCNNNNNNNGC 4 cut(s) 204, 607, 780, 834
NdeII GATC 7 cut(s) 12, 58, 148, 152, 217, 423, 765
NlaIII CATG 3 cut(s) 202, 227, 688
NlaIV GGNNCC 1 cut(s) 767
NmuCI GTSAC 6 cut(s) 168, 191, 269, 359, 443, 641
NspI RCATGY 2 cut(s) 202, 688
OliI CACNNNNGTG 1 cut(s) 510
PciI ACATGT 1 cut(s) 684
PcsI WCGNNNNNNNCGW 2 cut(s) 237, 792
PdmI GAANNNNTTC 1 cut(s) 402
PfeI GAWTC 2 cut(s) 125, 432
Pfl23II CGTACG 1 cut(s) 54
PflMI CCANNNNNTGG 1 cut(s) 696
PinAI ACCGGT 2 cut(s) 37, 747
PkrI GCNGC 4 cut(s) 141, 282, 335, 531
PleI GAGTC 1 cut(s) 560
PpsI GAGTC 1 cut(s) 560
Ppu21I YACGTR 1 cut(s) 497
PscI ACATGT 1 cut(s) 684
PsiI TTATAA 1 cut(s) 624
Psp124BI GAGCTC 2 cut(s) 134, 231
Psp6I CCWGG 1 cut(s) 695
PspGI CCWGG 1 cut(s) 695
PspLI CGTACG 1 cut(s) 54
PspN4I GGNNCC 1 cut(s) 767
PstI CTGCAG 1 cut(s) 431
PsuI RGATCY 2 cut(s) 423, 765
RsaI GTAC 2 cut(s) 56, 325
RsaNI GTAC 2 cut(s) 55, 324
RseI CAYNNNNRTG 2 cut(s) 411, 510
SacI GAGCTC 2 cut(s) 134, 231
SatI GCNGC 4 cut(s) 140, 281, 334, 530
Sau3AI GATC 7 cut(s) 12, 58, 148, 152, 217, 423, 765
SchI GAGTC 1 cut(s) 560
ScrFI CCNGG 1 cut(s) 697
SduI GDGCHC 3 cut(s) 134, 231, 603
SetI ASST 6 cut(s) 77, 134, 231, 499, 698, 762
SfaNI GCATC 2 cut(s) 108, 541
SfcI CTRYAG 1 cut(s) 427
SmiMI CAYNNNNRTG 2 cut(s) 411, 510
Sse9I AATT 3 cut(s) 371, 401, 449
SsiI CCGC 2 cut(s) 333, 597
SspI AATATT 2 cut(s) 353, 650
SstI GAGCTC 2 cut(s) 134, 231
StyD4I CCNGG 1 cut(s) 695
TaaI ACNGT 5 cut(s) 10, 23, 185, 586, 730
TaiI ACGT 1 cut(s) 499
TaqI TCGA 2 cut(s) 128, 151
TasI AATT 3 cut(s) 371, 401, 449
TatI WGTACW 1 cut(s) 323
TauI GCSGC 1 cut(s) 336
TfiI GAWTC 2 cut(s) 125, 432
TscAI CASTG 5 cut(s) 464, 542, 576, 617, 839
TseFI GTSAC 6 cut(s) 168, 191, 269, 359, 443, 641
TseI GCWGC 3 cut(s) 139, 280, 529
Tsp45I GTSAC 6 cut(s) 168, 191, 269, 359, 443, 641
TspDTI ATGAA 5 cut(s) 84, 395, 605, 642, 650
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 5 cut(s) 464, 542, 576, 617, 839
Van91I CCANNNNNTGG 1 cut(s) 696
XapI RAATTY 2 cut(s) 371, 449
XceI RCATGY 2 cut(s) 202, 688
XmiI GTMKAC 1 cut(s) 801
XmnI GAANNNNTTC 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.