Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008758

Physical Location & Seq

Genomic Coordinates Forward (+)

34355624 .. 34364755

Gene Structure 9132 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1267600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2667 bp

ATGGCGGAATCGGTGGAGCTTCCCACTCGCCTCGGCATCCTTCCTTTCCGAAACAAGGTCCTCCTACCGGGCGCCATCATTCGAATTCGCTGCACTTCACCCAGCAGTGTCAAATTGGTGGAGCAAGAGTTATGGCAGCGCGAAGAGAAGGGGTTAATTGGTATTCTTCCCGTTCGAGATGCTGCTGAGGCAGCAACTGTGGGCCCTGTGGTGTCTCAAGGTGTGGGAACTGATTCTGGTGAAAGAAGCTCTAGAGTTCAAGTTGGTGTATCCGATTCTCACAGGCTTGATGGAAAAAATCAGCAGGAAGTTATTCATTGGCATTCAAGGGGAGTTGCTGCACGGGCTTTACATCTCTCAAGAGGAGTTGAGAAGCCAAGTGGGAGGGTTACATATGTAGTTGTCCTTGAAGGTTTGTGTAGATTCAACATCCAGGAACTTAGCACAAGAGGAACATATTATACTGCACGAATTTCTCCACTTGAAATGACAAAGACTGAAATGGAGCAAGTGGAGCAAGATCCAGACTACATATCGTTGTCTCGCCAATTCAAAGCAACTGCTACGGAGCTGATTTCTGTTCTTGAGCAGAAACAAAAGACCGGAGGAAGAACAAAAGTTCTTCTGGAGACAGTTCCGGTTTACAAGTTGGCAGACATATTTGTTGCTAGCTTTGAGATAAGTTTTGAGGAGCAATTGTCTATGTTGGATTCAGTTGATCTGAAAGTAAGACTTTCCAAAGCTACTGAGCTAGTTGACAGGCATTTGCAGTCTATACATGTAGCAGAGAAGATTACACAAAAAGTTGAGGGTCAATTATCAAAATCGCAGAAAGAGTTTCTTTTGCGTCAGCAGATGAGAGCTATAAAAGAGGAGCTTGGCGACAATGATGATGACGAAGACGATGTAGTTGCCCTGGAAAGAAAGATGCAATGCTCAGGAATGCCTTCAAACATCTGGAAGCACGCACAGAGGGAGTTGAGGAGGCTGAAAAAGATGCAACCCCAACAACCTGGATATAATAGTTCACGTGTTTACCTAGACCTTCTTGCTGAACTTCCCTGGCAGAAGGCCAGTGAAGAATATGAATTGGATTTAAGGGCTGCAAAAGAACGTCTTGACAGTGATCACTATGGTTTAACTAAGGTCAAGCAGCGGATTATTGAGTATCTGGCTGTTCGCAAGCTCAAACCAGATGCCAGAGGTCCCGTGTTGTGTTTTGTTGGACCGCCCGGTGTTGGAAAAACGTCTTTGGCATCATCTATTGCTGCTTCTTTGGGCAGAAAATTTATACGCATATCCCTTGGTGGTGTTAAGGATGAGGCTGATATTCGAGGACACAGGAGGACATACATTGGAAGCATGCCGGGTAGACTCATTGATGGATTAAAGAGAGTAGCTGTTTGCAATCCAGTCATGCTGCTAGATGAAATTGACAAGACAGGTTCTGATGTGCGCGGTGATCCAGCTTCAGCTCTACTGGAGGTTCTTGATCCTGAACAAAATAAAACATTCAATGACCACTATTTGAATGTTCCCTTCGACCTTTCAAAGGTGATTTTTGTGGCAACTGCAAATAGGGTGCAGCCTATTCCGCCACCACTCTTGGACAGGATGGAAGTTATTGAGTTGCCTGGATATACACCTGAAGAAAAACTGAAGATAGCCATGCACCATTTAATTCCCCGAGTTCTTGATCAGCATGGTTTGAATTCTGAGTTTCTTAGAATCCCAGAGGCCATGGTGAAACTTGTCATTCAAGGGTACACTAGAGAGGCTGGTGTTCGGAATCTGGAGAGAAACTTGGCTGCCCTGGCTCGTGCAGCTGCAGTAAGAGTTGCCGAGCAAGAACAAGCTGTCTCTCTGAGCAAAGATGTGCAATCACTTGCTTCACCACTACTCGAAAACAGACTTGCTGATGGAGCTGAAGTGGAAATGGAGGTTATTCCGATGGGTGTGAATAATCATGAGATATCAAGCACTTTCAAGATTGCTTCACCATTGACTGTGGATGAGGATATGCTGGAGAAAGTATTAGGGCCTCCAAAGTTTGATGACAAAGAAGCTGCAGAACGAGTTGCGACACCTGGTGTATCTGTTGGGCTTGTTTGGACTGCTTTCGGTGGAGAGGTCCAATTTGTGGAGGCTACAGCAATGGGGGGAAAGGGTGAATTACATCTCACTGGTCAACTTGGAGATGTTATCAAAGAATCAGCACAAATAGCATTGACATGGGTGAGAGCCAGGGCAAGGGATCTTAACTTGTCAACAGCTGAGGCAACCAATCTTTTGGAAGGTCGTGATGTTCACATACACTTTCCTGCTGGGGCTGTACCCAAGGATGGACCCTCAGCGGGTGTTACTCTGGTAACTGCACTGGTTTCGTTGTTCAGTGAGAGAAGAGTAAGAGCAGATACAGCAATGACTGGAGAAATGACATTGAGGGGTCTAGTACTACCTGTTGGTGGTGTCAAGGATAAGGTATTGGCAGCCCACCGTTGTGGTATTAAAAGAGTCATTCTTCCGGAGAGGAATCTGAAGGACTTAACTGAAGTACCATCGGCGGTTCTTTCCGGTCTTGAGATAATACTGGCAAAGCGAATGGAAGATGTGTTAGAGCAGGCTTTTGAAGGCGGATGTCCCTGGAGACAGCACTCAAAGTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000002 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0006139 GO:0006259 GO:0006508 GO:0006605 GO:0006625 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0006996 GO:0007005 GO:0007031 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009628 GO:0009791 GO:0009987 GO:0010467 GO:0015031 GO:0015833 GO:0015919 GO:0016043 GO:0016485 GO:0016558 GO:0016560 GO:0017038 GO:0019538 GO:0022622 GO:0031907 GO:0031974 GO:0032042 GO:0032501 GO:0032502 GO:0033036 GO:0033365 GO:0034613 GO:0034641 GO:0040007 GO:0042579 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043574 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0044743 GO:0045184 GO:0046483 GO:0046907 GO:0048364 GO:0048527 GO:0048528 GO:0048731 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0051604 GO:0051641 GO:0051649 GO:0055085 GO:0065002 GO:0070013 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0090304 GO:0090696 GO:0099402 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04112 map04112

Pfam Domains

Protein Families

AAA LON_substr_bdg Lon_C

Protein Analysis

889

Amino Acids

98.09

Weight (kDa)

6.49

Isoelectric Point (pI)

39.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LON_substr_bdg	PF02190	11 - 254	2.7e-29	ATP-dependent protease La (LON) substrate-binding domain
AAA	PF00004	405 - 542	4.1e-25	ATPase family associated with various cellular activities (AAA)
AAA_5	PF07728	405 - 539	1.9e-07	AAA domain (dynein-related subfamily)
Lon_lid	PF22667	567 - 618	3.6e-17	Lon protease AAA+ ATPase lid domain
Lon_C	PF05362	670 - 876	1.7e-77	Lon protease (S16) C-terminal proteolytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008758)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G47040
fragaria_vesca	FvH4_5g20910 FvH4_5g20910
malus_domestica	MD06G1043200.v1.1 MD16G1267600.v1.1
prunus_persica	Prupe.5G050100_v2.0.a1 Prupe.5G050100_v2.0.a1
pyrus_communis	pycom06g03620 pycom16g23700 pycom16g23710
rosa_chinensis	RchiOBHm_Chr7g0207901
rosa_laevigata	RLG00000003277
rosa_multiflora	Rmu_co8377115.1_g000001 Rmu_sc0006577.1_g000003
rosa_roxburghii	Rroxscaffold_3G00251060
rosa_rugosa	Rorug07G0100200 Rorug07G0100200
rosa_samantha	Rh7AG233500 Rh7BG229100 Rh7CG248800 Rh7DG239500
rosa_wichuraiana	Rw0G001300 Rw7G020040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	71
AccB7I	CCANNNNNTGG	1 cut(s)	2140
AccI	GTMKAC	1 cut(s)	1372
AccII	CGCG	2 cut(s)	141, 1458
AccIII	TCCGGA	1 cut(s)	2524
AciI	CCGC	8 cut(s)	5, 1156, 1229, 1458, 1595, 2354, 2564, 2634
AclWI	GGATC	4 cut(s)	515, 1457, 1487, 2262
AcsI	RAATTY	4 cut(s)	84, 471, 1286, 1711
AcuI	CTGAAG	6 cut(s)	1455, 1668, 1679, 1947, 2558, 2571
AcvI	CACGTG	1 cut(s)	1031
AcyI	GRCGYC	1 cut(s)	72
AdeI	CACNNNGTG	1 cut(s)	2090
AfaI	GTAC	4 cut(s)	1766, 2334, 2454, 2556
AfiI	CCNNNNNNNGG	9 cut(s)	55, 67, 1238, 1552, 2140, 2250, 2342, 2354, 2465
AflIII	ACRYGT	2 cut(s)	778, 1030
AjnI	CCWGG	9 cut(s)	432, 915, 1012, 1061, 1633, 1812, 2086, 2243, 2642
AleI	CACNNNNGTG	1 cut(s)	2499
Alw26I	GTCTC	5 cut(s)	219, 546, 623, 1864, 2641
AlwI	GGATC	4 cut(s)	515, 1457, 1487, 2262
AlwNI	CAGNNNCTG	2 cut(s)	197, 1448
Ama87I	CYCGRG	1 cut(s)	1686
Aor13HI	TCCGGA	1 cut(s)	2524
AoxI	GGCC	4 cut(s)	202, 1071, 1737, 2039
ApaI	GGGCCC	1 cut(s)	206
ApoI	RAATTY	4 cut(s)	84, 471, 1286, 1711
Asp700I	GAANNNNTTC	3 cut(s)	232, 312, 946
AspLEI	GCGC	3 cut(s)	74, 141, 1458
AspS9I	GGNCC	8 cut(s)	58, 202, 203, 1205, 1226, 2039, 2131, 2345
AsuC2I	CCSGG	3 cut(s)	69, 1233, 1368
AsuHPI	GGTGA	9 cut(s)	90, 251, 1472, 1567, 1756, 1884, 1989, 2180, 2248
AsuII	TTCGAA	1 cut(s)	82
AsuNHI	GCTAGC	1 cut(s)	668
AvaI	CYCGRG	1 cut(s)	1686
AvaII	GGWCC	5 cut(s)	58, 1205, 1226, 2131, 2345
BaeGI	GKGCMC	1 cut(s)	206
BaeI	ACNNNNGTAYC	2 cut(s)	2538, 2571
BanI	GGYRCC	1 cut(s)	71
BanII	GRGCYC	1 cut(s)	206
BauI	CACGAG	1 cut(s)	1818
BbrPI	CACGTG	1 cut(s)	1031
BbsI	GAAGAC	1 cut(s)	906
BbvCI	CCTCAGC	3 cut(s)	186, 2274, 2350
BccI	CCATC	8 cut(s)	83, 284, 1376, 1609, 1913, 1945, 2336, 2566
BcgI	CGANNNNNNTGC	6 cut(s)	72, 106, 893, 927, 2364, 2398
BciT130I	CCWGG	9 cut(s)	434, 917, 1014, 1063, 1635, 1814, 2088, 2245, 2644
BciVI	GTATCC	1 cut(s)	280
BclI	TGATCA	2 cut(s)	1126, 1696
BcnI	CCSGG	3 cut(s)	69, 1233, 1368
BcoDI	GTCTC	5 cut(s)	219, 546, 623, 1864, 2641
BfaI	CTAG	7 cut(s)	252, 669, 752, 1040, 1424, 1770, 2450
BfmI	CTRYAG	3 cut(s)	1827, 2067, 2148
BfoI	RGCGCY	1 cut(s)	75
BfuI	GTATCC	1 cut(s)	280
BmcAI	AGTACT	1 cut(s)	2454
Bme18I	GGWCC	5 cut(s)	58, 1205, 1226, 2131, 2345
BmeT110I	CYCGRG	1 cut(s)	1686
BmgT120I	GGNCC	8 cut(s)	58, 202, 203, 1205, 1226, 2039, 2131, 2345
BmiI	GGNNCC	4 cut(s)	73, 204, 1207, 2347
BmsI	GCATC	6 cut(s)	45, 169, 918, 987, 1186, 1265
BmtI	GCTAGC	1 cut(s)	672
BpiI	GAAGAC	1 cut(s)	906
BpmI	CTGGAG	6 cut(s)	647, 1502, 1814, 2045, 2448, 2665
Bpu10I	CCTNAGC	4 cut(s)	186, 937, 2274, 2350
Bpu14I	TTCGAA	1 cut(s)	82
BpuEI	CTTGAG	4 cut(s)	201, 343, 605, 2600
BpuMI	CCSGG	3 cut(s)	69, 1233, 1368
BsaAI	YACGTR	1 cut(s)	1031
BsaHI	GRCGYC	1 cut(s)	72
BsaJI	CCNNGG	9 cut(s)	31, 915, 1061, 1303, 1740, 1812, 2244, 2337, 2642
BsaWI	WCCGGW	4 cut(s)	602, 637, 2524, 2573
BsaXI	ACNNNNNCTCC	6 cut(s)	113, 143, 683, 713, 1914, 1944
Bsc4I	CCNNNNNNNGG	9 cut(s)	55, 67, 1238, 1552, 2140, 2250, 2342, 2354, 2465
Bse1I	ACTGG	7 cut(s)	1074, 1412, 1485, 2188, 2382, 2431, 2595
Bse3DI	GCAATG	3 cut(s)	938, 2160, 2427
BseAI	TCCGGA	1 cut(s)	2524
BseBI	CCWGG	9 cut(s)	434, 917, 1014, 1063, 1635, 1814, 2088, 2245, 2644
BseDI	CCNNGG	9 cut(s)	31, 915, 1061, 1303, 1740, 1812, 2244, 2337, 2642
BseGI	GGATG	7 cut(s)	36, 429, 1324, 1620, 2017, 2347, 2642
BseLI	CCNNNNNNNGG	9 cut(s)	55, 67, 1238, 1552, 2140, 2250, 2342, 2354, 2465
BseMI	GCAATG	3 cut(s)	938, 2160, 2427
BseMII	CTCAG	7 cut(s)	177, 738, 951, 1707, 1856, 2265, 2364
BseNI	ACTGG	7 cut(s)	1074, 1412, 1485, 2188, 2382, 2431, 2595
BseRI	GAGGAG	4 cut(s)	378, 704, 887, 997
BseSI	GKGCMC	1 cut(s)	206
BseYI	CCCAGC	2 cut(s)	101, 2324
BsgI	GTGCAG	6 cut(s)	76, 324, 450, 1604, 1842, 2358
Bsh1236I	CGCG	2 cut(s)	141, 1458
BshFI	GGCC	4 cut(s)	204, 1073, 1739, 2041
BshNI	GGYRCC	1 cut(s)	71
BsiHKCI	CYCGRG	1 cut(s)	1686
BsiSI	CCGG	7 cut(s)	68, 603, 638, 1233, 1367, 2525, 2574
BslFI	GGGAC	2 cut(s)	1191, 2625
BslI	CCNNNNNNNGG	9 cut(s)	55, 67, 1238, 1552, 2140, 2250, 2342, 2354, 2465
BsmAI	GTCTC	5 cut(s)	219, 546, 623, 1864, 2641
BsmFI	GGGAC	2 cut(s)	1191, 2625
BsmI	GAATGC	2 cut(s)	322, 948
BsnI	GGCC	4 cut(s)	204, 1073, 1739, 2041
BsoBI	CYCGRG	1 cut(s)	1686
Bsp119I	TTCGAA	1 cut(s)	82
Bsp120I	GGGCCC	1 cut(s)	202
Bsp1286I	GDGCHC	1 cut(s)	206
Bsp13I	TCCGGA	1 cut(s)	2524
Bsp143I	GATC	7 cut(s)	520, 718, 1126, 1462, 1492, 1696, 2254
Bsp19I	CCATGG	1 cut(s)	1740
BspACI	CCGC	8 cut(s)	5, 1156, 1229, 1458, 1595, 2354, 2564, 2634
BspANI	GGCC	4 cut(s)	204, 1073, 1739, 2041
BspCNI	CTCAG	7 cut(s)	178, 739, 950, 1708, 1857, 2266, 2363
BspEI	TCCGGA	1 cut(s)	2524
BspFNI	CGCG	2 cut(s)	141, 1458
BspHI	TCATGA	1 cut(s)	1966
BspLI	GGNNCC	4 cut(s)	73, 204, 1207, 2347
BspMAI	CTGCAG	2 cut(s)	1831, 2071
BspOI	GCTAGC	1 cut(s)	672
BspPI	GGATC	4 cut(s)	515, 1457, 1487, 2262
BspT104I	TTCGAA	1 cut(s)	82
BspT107I	GGYRCC	1 cut(s)	71
BsrDI	GCAATG	3 cut(s)	938, 2160, 2427
BsrI	ACTGG	7 cut(s)	1074, 1412, 1485, 2188, 2382, 2431, 2595
BssECI	CCNNGG	9 cut(s)	31, 915, 1061, 1303, 1740, 1812, 2244, 2337, 2642
BssMI	GATC	7 cut(s)	520, 718, 1126, 1462, 1492, 1696, 2254
BssNI	GRCGYC	1 cut(s)	72
BssSI	CACGAG	1 cut(s)	1818
BssT1I	CCWWGG	3 cut(s)	1303, 1740, 2337
Bst2BI	CACGAG	1 cut(s)	1818
Bst2UI	CCWGG	9 cut(s)	434, 917, 1014, 1063, 1635, 1814, 2088, 2245, 2644
Bst4CI	ACNGT	5 cut(s)	199, 634, 1124, 2008, 2498
Bst6I	CTCTTC	2 cut(s)	138, 2395
BstACI	GRCGYC	1 cut(s)	72
BstBAI	YACGTR	1 cut(s)	1031
BstBI	TTCGAA	1 cut(s)	82
BstC8I	GCNNGC	5 cut(s)	670, 966, 1184, 1364, 2622
BstDSI	CCRYGG	1 cut(s)	1740
BstENI	CCTNNNNNAGG	1 cut(s)	1550
BstF5I	GGATG	7 cut(s)	36, 429, 1324, 1620, 2017, 2347, 2642
BstFNI	CGCG	2 cut(s)	141, 1458
BstH2I	RGCGCY	1 cut(s)	75
BstHHI	GCGC	3 cut(s)	74, 141, 1458
BstKTI	GATC	7 cut(s)	523, 721, 1129, 1465, 1495, 1699, 2257
BstMAI	GTCTC	5 cut(s)	219, 546, 623, 1864, 2641
BstMBI	GATC	7 cut(s)	520, 718, 1126, 1462, 1492, 1696, 2254
BstNI	CCWGG	9 cut(s)	434, 917, 1014, 1063, 1635, 1814, 2088, 2245, 2644
BstNSI	RCATGY	2 cut(s)	782, 1366
BstSFI	CTRYAG	3 cut(s)	1827, 2067, 2148
BstSLI	GKGCMC	1 cut(s)	206
BstUI	CGCG	2 cut(s)	141, 1458
BstV2I	GAAGAC	1 cut(s)	906
BstX2I	RGATCY	2 cut(s)	520, 2254
BstXI	CCANNNNNNTGG	3 cut(s)	1013, 2290, 2501
BstYI	RGATCY	2 cut(s)	520, 2254
BsuI	GTATCC	1 cut(s)	280
BsuRI	GGCC	4 cut(s)	204, 1073, 1739, 2041
BtgI	CCRYGG	1 cut(s)	1740
BtsCI	GGATG	7 cut(s)	36, 429, 1324, 1620, 2017, 2347, 2642
BtsI	GCAGTG	1 cut(s)	112
BtsIMutI	CAGTG	6 cut(s)	112, 1081, 1129, 2181, 2375, 2398
Cac8I	GCNNGC	5 cut(s)	670, 966, 1184, 1364, 2622
CaiI	CAGNNNCTG	2 cut(s)	197, 1448
CciI	TCATGA	1 cut(s)	1966
CfoI	GCGC	3 cut(s)	74, 141, 1458
Cfr13I	GGNCC	8 cut(s)	58, 202, 203, 1205, 1226, 2039, 2131, 2345
CseI	GACGC	1 cut(s)	836
CsiI	ACCWGGT	1 cut(s)	2086
Csp6I	GTAC	4 cut(s)	1765, 2333, 2453, 2555
CspCI	CAANNNNNGTGG	2 cut(s)	1587, 1622
CviAII	CATG	8 cut(s)	779, 1363, 1417, 1669, 1703, 1741, 1967, 2232
CviQI	GTAC	4 cut(s)	1765, 2333, 2453, 2555
DinI	GGCGCC	1 cut(s)	73
DpnI	GATC	7 cut(s)	522, 720, 1128, 1464, 1494, 1698, 2256
DpnII	GATC	7 cut(s)	520, 718, 1126, 1462, 1492, 1696, 2254
DraIII	CACNNNGTG	1 cut(s)	2090
Eam1104I	CTCTTC	2 cut(s)	138, 2395
EarI	CTCTTC	2 cut(s)	138, 2395
EciI	GGCGGA	3 cut(s)	20, 1584, 2649
Eco130I	CCWWGG	3 cut(s)	1303, 1740, 2337
Eco24I	GRGCYC	1 cut(s)	206
Eco32I	GATATC	1 cut(s)	1974
Eco47I	GGWCC	5 cut(s)	58, 1205, 1226, 2131, 2345
Eco57I	CTGAAG	6 cut(s)	1455, 1668, 1679, 1947, 2558, 2571
Eco72I	CACGTG	1 cut(s)	1031
Eco88I	CYCGRG	1 cut(s)	1686
EcoNI	CCTNNNNNAGG	1 cut(s)	1550
EcoO109I	RGGNCCY	4 cut(s)	58, 203, 1205, 2039
EcoRI	GAATTC	2 cut(s)	84, 1711
EcoRII	CCWGG	9 cut(s)	432, 915, 1012, 1061, 1633, 1812, 2086, 2243, 2642
EcoRV	GATATC	1 cut(s)	1974
EcoT14I	CCWWGG	3 cut(s)	1303, 1740, 2337
EcoT38I	GRGCYC	1 cut(s)	206
EgeI	GGCGCC	1 cut(s)	73
EheI	GGCGCC	1 cut(s)	73
ErhI	CCWWGG	3 cut(s)	1303, 1740, 2337
FaeI	CATG	8 cut(s)	782, 1366, 1420, 1672, 1706, 1744, 1970, 2235
FalI	AAGNNNNNCTT	8 cut(s)	717, 749, 825, 857, 861, 893, 1101, 1133
FaqI	GGGAC	2 cut(s)	1191, 2625
FatI	CATG	8 cut(s)	778, 1362, 1416, 1668, 1702, 1740, 1966, 2231
FauI	CCCGC	1 cut(s)	2347
FauNDI	CATATG	1 cut(s)	394
FbaI	TGATCA	2 cut(s)	1126, 1696
FblI	GTMKAC	1 cut(s)	1372
FokI	GGATG	7 cut(s)	23, 416, 1331, 1627, 2024, 2354, 2649
FriOI	GRGCYC	1 cut(s)	206
FspBI	CTAG	7 cut(s)	252, 669, 752, 1040, 1424, 1770, 2450
GlaI	GCGC	3 cut(s)	73, 140, 1457
GsaI	CCCAGC	2 cut(s)	105, 2328
GsuI	CTGGAG	6 cut(s)	647, 1502, 1814, 2045, 2448, 2665
HaeII	RGCGCY	1 cut(s)	75
HaeIII	GGCC	4 cut(s)	204, 1073, 1739, 2041
HapII	CCGG	7 cut(s)	68, 603, 638, 1233, 1367, 2525, 2574
HgaI	GACGC	1 cut(s)	836
HhaI	GCGC	3 cut(s)	74, 141, 1458
Hin1I	GRCGYC	1 cut(s)	72
Hin1II	CATG	8 cut(s)	782, 1366, 1420, 1672, 1706, 1744, 1970, 2235
Hin6I	GCGC	3 cut(s)	72, 139, 1456
HinP1I	GCGC	3 cut(s)	72, 139, 1456
HincII	GTYRAC	3 cut(s)	757, 2189, 2268
HindII	GTYRAC	3 cut(s)	757, 2189, 2268
HpaII	CCGG	7 cut(s)	68, 603, 638, 1233, 1367, 2525, 2574
HphI	GGTGA	9 cut(s)	90, 251, 1472, 1567, 1756, 1884, 1989, 2180, 2248
Hpy166II	GTNNAC	9 cut(s)	643, 757, 1028, 1036, 1373, 1767, 2189, 2268, 2308
Hpy188I	TCNGA	9 cut(s)	50, 274, 723, 1450, 1717, 1788, 1866, 1950, 2538
Hpy8I	GTNNAC	9 cut(s)	643, 757, 1028, 1036, 1373, 1767, 2189, 2268, 2308
HpyCH4III	ACNGT	5 cut(s)	199, 634, 1124, 2008, 2498
HpyCH4IV	ACGT	3 cut(s)	1030, 1114, 1247
HpySE526I	ACGT	3 cut(s)	1030, 1114, 1247
Hsp92I	GRCGYC	1 cut(s)	72
Hsp92II	CATG	8 cut(s)	782, 1366, 1420, 1672, 1706, 1744, 1970, 2235
HspAI	GCGC	3 cut(s)	72, 139, 1456
KasI	GGCGCC	1 cut(s)	71
Kpn2I	TCCGGA	1 cut(s)	2524
Ksp22I	TGATCA	2 cut(s)	1126, 1696
Kzo9I	GATC	7 cut(s)	520, 718, 1126, 1462, 1492, 1696, 2254
LmnI	GCTCC	8 cut(s)	16, 121, 505, 514, 568, 691, 874, 1922
LweI	GCATC	6 cut(s)	45, 169, 918, 987, 1186, 1265
MabI	ACCWGGT	1 cut(s)	2086
MaeI	CTAG	7 cut(s)	252, 669, 752, 1040, 1424, 1770, 2450
MaeII	ACGT	3 cut(s)	1030, 1114, 1247
MaeIII	GTNAC	3 cut(s)	388, 2359, 2368
MalI	GATC	7 cut(s)	522, 720, 1128, 1464, 1494, 1698, 2256
MboI	GATC	7 cut(s)	520, 718, 1126, 1462, 1492, 1696, 2254
MfeI	CAATTG	1 cut(s)	695
MflI	RGATCY	2 cut(s)	520, 2254
MhlI	GDGCHC	1 cut(s)	206
Mly113I	GGCGCC	1 cut(s)	72
MlyI	GAGTC	2 cut(s)	1368, 2523
MmeI	TCCRAC	3 cut(s)	687, 1204, 1219
MroI	TCCGGA	1 cut(s)	2524
MroXI	GAANNNNTTC	3 cut(s)	232, 312, 946
MseI	TTAA	9 cut(s)	155, 1097, 1139, 1314, 1388, 1679, 2259, 2508, 2546
MslI	CAYNNNNRTG	2 cut(s)	2230, 2499
MspA1I	CMGCKG	4 cut(s)	1156, 1826, 2273, 2354
MspI	CCGG	7 cut(s)	68, 603, 638, 1233, 1367, 2525, 2574
MunI	CAATTG	1 cut(s)	695
Mva1269I	GAATGC	2 cut(s)	322, 948
MvaI	CCWGG	9 cut(s)	434, 917, 1014, 1063, 1635, 1814, 2088, 2245, 2644
MvnI	CGCG	2 cut(s)	141, 1458
NarI	GGCGCC	1 cut(s)	72
NciI	CCSGG	3 cut(s)	69, 1233, 1368
NcoI	CCATGG	1 cut(s)	1740
NdeI	CATATG	1 cut(s)	394
NdeII	GATC	7 cut(s)	520, 718, 1126, 1462, 1492, 1696, 2254
NheI	GCTAGC	1 cut(s)	668
NlaIII	CATG	8 cut(s)	782, 1366, 1420, 1672, 1706, 1744, 1970, 2235
NlaIV	GGNNCC	4 cut(s)	73, 204, 1207, 2347
NmeAIII	GCCGAG	2 cut(s)	12, 1867
NspI	RCATGY	2 cut(s)	782, 1366
NspV	TTCGAA	1 cut(s)	82
OliI	CACNNNNGTG	1 cut(s)	2499
PaeI	GCATGC	1 cut(s)	1366
PagI	TCATGA	1 cut(s)	1966
PciI	ACATGT	1 cut(s)	778
PctI	GAATGC	2 cut(s)	322, 948
PdmI	GAANNNNTTC	3 cut(s)	232, 312, 946
PfeI	GAWTC	9 cut(s)	8, 233, 275, 423, 710, 1728, 1789, 2210, 2533
PflMI	CCANNNNNTGG	1 cut(s)	2140
PfoI	TCCNGGA	1 cut(s)	432
PleI	GAGTC	2 cut(s)	1368, 2522
PluTI	GGCGCC	1 cut(s)	75
PmaCI	CACGTG	1 cut(s)	1031
PmlI	CACGTG	1 cut(s)	1031
PpsI	GAGTC	2 cut(s)	1368, 2522
Ppu21I	YACGTR	1 cut(s)	1031
PpuMI	RGGWCCY	2 cut(s)	58, 1205
PscI	ACATGT	1 cut(s)	778
Psp5II	RGGWCCY	2 cut(s)	58, 1205
Psp6I	CCWGG	9 cut(s)	432, 915, 1012, 1061, 1633, 1812, 2086, 2243, 2642
PspCI	CACGTG	1 cut(s)	1031
PspFI	CCCAGC	2 cut(s)	101, 2324
PspGI	CCWGG	9 cut(s)	432, 915, 1012, 1061, 1633, 1812, 2086, 2243, 2642
PspN4I	GGNNCC	4 cut(s)	73, 204, 1207, 2347
PspOMI	GGGCCC	1 cut(s)	202
PspPI	GGNCC	8 cut(s)	58, 202, 203, 1205, 1226, 2039, 2131, 2345
PspPPI	RGGWCCY	2 cut(s)	58, 1205
PsrI	GAACNNNNNNTAC	2 cut(s)	445, 477
PstI	CTGCAG	2 cut(s)	1831, 2071
PstNI	CAGNNNCTG	2 cut(s)	197, 1448
PsuI	RGATCY	2 cut(s)	520, 2254
PvuII	CAGCTG	2 cut(s)	1826, 2273
RsaI	GTAC	4 cut(s)	1766, 2334, 2454, 2556
RsaNI	GTAC	4 cut(s)	1765, 2333, 2453, 2555
RseI	CAYNNNNRTG	2 cut(s)	2230, 2499
SaqAI	TTAA	9 cut(s)	155, 1097, 1139, 1314, 1388, 1679, 2259, 2508, 2546
Sau3AI	GATC	7 cut(s)	520, 718, 1126, 1462, 1492, 1696, 2254
Sau96I	GGNCC	8 cut(s)	58, 202, 203, 1205, 1226, 2039, 2131, 2345
ScaI	AGTACT	1 cut(s)	2454
SchI	GAGTC	2 cut(s)	1368, 2523
SduI	GDGCHC	1 cut(s)	206
SexAI	ACCWGGT	1 cut(s)	2086
SfaNI	GCATC	6 cut(s)	45, 169, 918, 987, 1186, 1265
SfcI	CTRYAG	3 cut(s)	1827, 2067, 2148
SfoI	GGCGCC	1 cut(s)	73
SfuI	TTCGAA	1 cut(s)	82
SinI	GGWCC	5 cut(s)	58, 1205, 1226, 2131, 2345
SmiMI	CAYNNNNRTG	2 cut(s)	2230, 2499
SmlI	CTYRAG	4 cut(s)	216, 358, 584, 2579
SmoI	CTYRAG	4 cut(s)	216, 358, 584, 2579
SphI	GCATGC	1 cut(s)	1366
SsiI	CCGC	8 cut(s)	5, 1156, 1229, 1458, 1595, 2354, 2564, 2634
SspDI	GGCGCC	1 cut(s)	71
SspMI	CTAG	7 cut(s)	252, 669, 752, 1040, 1424, 1770, 2450
StyI	CCWWGG	3 cut(s)	1303, 1740, 2337
TaaI	ACNGT	5 cut(s)	199, 634, 1124, 2008, 2498
TaiI	ACGT	3 cut(s)	1033, 1117, 1250
TaqI	TCGA	5 cut(s)	82, 175, 1333, 1542, 1902
TatI	WGTACW	1 cut(s)	2452
TfiI	GAWTC	9 cut(s)	8, 233, 275, 423, 710, 1728, 1789, 2210, 2533
Tru1I	TTAA	9 cut(s)	155, 1097, 1139, 1314, 1388, 1679, 2259, 2508, 2546
Tru9I	TTAA	9 cut(s)	155, 1097, 1139, 1314, 1388, 1679, 2259, 2508, 2546
TscAI	CASTG	6 cut(s)	112, 1081, 1129, 2188, 2382, 2398
TspDTI	ATGAA	3 cut(s)	305, 1101, 1443
TspGWI	ACGGA	1 cut(s)	581
TspRI	CASTG	6 cut(s)	112, 1081, 1129, 2188, 2382, 2398
Van91I	CCANNNNNTGG	1 cut(s)	2140
VpaK11BI	GGWCC	5 cut(s)	58, 1205, 1226, 2131, 2345
XagI	CCTNNNNNAGG	1 cut(s)	1550
XapI	RAATTY	4 cut(s)	84, 471, 1286, 1711
XbaI	TCTAGA	1 cut(s)	251
XceI	RCATGY	2 cut(s)	782, 1366
XmiI	GTMKAC	1 cut(s)	1372
XmnI	GAANNNNTTC	3 cut(s)	232, 312, 946
XspI	CTAG	7 cut(s)	252, 669, 752, 1040, 1424, 1770, 2450
ZrmI	AGTACT	1 cut(s)	2454

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.