MD17G1011000.v1.1

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015014
Physical Location & Seq
Reverse (-)
677846 .. 678988
1143 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1011000.v1.1.491

Sequence Viewer

Length: 543 bp
ATGAATCCAGTAGTTCTCGTGGCCATTTCATCTTTCTTCTTCCTCCAGTCAGTAGTGTTTCTGGGAGGAGTTGAAGCAGCAGCAAAAGTCACTAATAAGGCAGTAGCAAAAGGTAACTCCCAGATCATTGTAATGGGCCTTGTTTACTGTGACATCTGCTACAACAACACCTTCTCCAAACACAGCTACTTCCTACCAGGTGCTGAAGTAAAAATCGATTGCATCTTCCAAGCGGTTTCGCCAAGAACCTCAGAGCACATATCGTTCTCGGTGAATAGAACTACAAACAGATATGGGGTGTACAAGTTGGAAATCCCATCTGTTGAGGGGATCAAATGTGCAGAGGATTCAGCGATTGTATCTTCCTGCCAGGCAAGCTTGATGTGGAGTTCTTCTTCTGCATGCAATGTTCCTGGTTACAAATCCACATCAAATGAAATAGCAGTCAAATCGAAACAGGCCAATCTCTGCATCTACAGCCTAAACGCTTTGAATTTCAGACCTTCAAAGAGAGATGTTAAATTGTGTGGGAAGAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

181

Amino Acids

19.59

Weight (kDa)

9.21

Isoelectric Point (pI)

35.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 44 - 142 5.4e-21 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015014)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26960 AT5G41050
fragaria_vesca FvH4_6g53930
malus_domestica MD17G1011000.v1.1
prunus_persica Prupe.3G309900_v2.0.a1
pyrus_communis pycom17g00570
rosa_chinensis RchiOBHm_Chr2g0176091
rosa_laevigata RLG00000022380
rosa_multiflora Rmu_sc0009879.1_g000002
rosa_roxburghii Rroxscaffold_2G00076780
rosa_rugosa Rorug02G0590400
rosa_samantha Rh2AG669200 Rh2BG680800 Rh2CG643700 Rh2DG694500
rosa_wichuraiana Rw2G054890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 233
AclWI GGATC 1 cut(s) 338
AcoI YGGCCR 1 cut(s) 21
AcsI RAATTY 1 cut(s) 493
AcuI CTGAAG 1 cut(s) 225
AfaI GTAC 1 cut(s) 302
AgsI TTSAA 3 cut(s) 74, 493, 507
AjnI CCWGG 3 cut(s) 196, 369, 412
AluBI AGCT 2 cut(s) 186, 378
AluI AGCT 2 cut(s) 186, 378
Alw21I GWGCWC 1 cut(s) 258
AlwI GGATC 1 cut(s) 338
AlwNI CAGNNNCTG 1 cut(s) 203
AoxI GGCC 3 cut(s) 21, 136, 459
ApeKI GCWGC 2 cut(s) 77, 80
ApoI RAATTY 1 cut(s) 493
AspS9I GGNCC 1 cut(s) 136
AsuHPI GGTGA 1 cut(s) 283
BalI TGGCCA 1 cut(s) 23
BauI CACGAG 1 cut(s) 17
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 2 cut(s) 89, 92
BccI CCATC 1 cut(s) 325
BcgI CGANNNNNNTGC 2 cut(s) 432, 466
BciT130I CCWGG 3 cut(s) 198, 371, 414
BfmI CTRYAG 1 cut(s) 475
BisI GCNGC 2 cut(s) 78, 81
BlsI GCNGC 2 cut(s) 79, 82
Bme1390I CCNGG 3 cut(s) 198, 371, 414
BmgT120I GGNCC 1 cut(s) 136
BmrFI CCNGG 3 cut(s) 198, 371, 414
BmsI GCATC 2 cut(s) 231, 480
BpmI CTGGAG 1 cut(s) 29
Bsa29I ATCGAT 1 cut(s) 216
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bse1I ACTGG 2 cut(s) 8, 46
Bse3DI GCAATG 1 cut(s) 412
BseBI CCWGG 3 cut(s) 198, 371, 414
BseCI ATCGAT 1 cut(s) 216
BseMI GCAATG 1 cut(s) 412
BseMII CTCAG 1 cut(s) 264
BseNI ACTGG 2 cut(s) 8, 46
BseRI GAGGAG 1 cut(s) 81
BseXI GCAGC 2 cut(s) 89, 92
BsgI GTGCAG 1 cut(s) 360
BshFI GGCC 3 cut(s) 23, 138, 461
BshVI ATCGAT 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 258
BsnI GGCC 3 cut(s) 23, 138, 461
Bsp1286I GDGCHC 1 cut(s) 258
Bsp1407I TGTACA 1 cut(s) 300
Bsp143I GATC 2 cut(s) 123, 330
BspACI CCGC 1 cut(s) 233
BspANI GGCC 3 cut(s) 23, 138, 461
BspCNI CTCAG 1 cut(s) 263
BspDI ATCGAT 1 cut(s) 216
BspPI GGATC 1 cut(s) 338
BsrDI GCAATG 1 cut(s) 412
BsrGI TGTACA 1 cut(s) 300
BsrI ACTGG 2 cut(s) 8, 46
BssMI GATC 2 cut(s) 123, 330
BssSI CACGAG 1 cut(s) 17
Bst2BI CACGAG 1 cut(s) 17
Bst2UI CCWGG 3 cut(s) 198, 371, 414
Bst4CI ACNGT 1 cut(s) 149
BstAUI TGTACA 1 cut(s) 300
BstC8I GCNNGC 2 cut(s) 376, 403
BstDEI CTNAG 1 cut(s) 250
BstKTI GATC 2 cut(s) 126, 333
BstMBI GATC 2 cut(s) 123, 330
BstMWI GCNNNNNNNGC 2 cut(s) 375, 477
BstNI CCWGG 3 cut(s) 198, 371, 414
BstNSI RCATGY 1 cut(s) 405
BstSCI CCNGG 3 cut(s) 196, 369, 412
BstSFI CTRYAG 1 cut(s) 475
BstV1I GCAGC 2 cut(s) 89, 92
Bsu15I ATCGAT 1 cut(s) 216
BsuRI GGCC 3 cut(s) 23, 138, 461
BsuTUI ATCGAT 1 cut(s) 216
Cac8I GCNNGC 2 cut(s) 376, 403
CaiI CAGNNNCTG 1 cut(s) 203
Cfr13I GGNCC 1 cut(s) 136
ClaI ATCGAT 1 cut(s) 216
CsiI ACCWGGT 1 cut(s) 196
Csp6I GTAC 1 cut(s) 301
CviAII CATG 1 cut(s) 402
CviJI RGCY 6 cut(s) 23, 138, 186, 378, 461, 480
CviKI_1 RGCY 6 cut(s) 23, 138, 186, 378, 461, 480
CviQI GTAC 1 cut(s) 301
DdeI CTNAG 1 cut(s) 250
DpnI GATC 2 cut(s) 125, 332
DpnII GATC 2 cut(s) 123, 330
EaeI YGGCCR 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 225
EcoRII CCWGG 3 cut(s) 196, 369, 412
FaeI CATG 1 cut(s) 405
FaiI YATR 3 cut(s) 260, 294, 403
FatI CATG 1 cut(s) 401
Fnu4HI GCNGC 2 cut(s) 78, 81
Fsp4HI GCNGC 2 cut(s) 78, 81
GluI GCNGC 2 cut(s) 78, 81
GsuI CTGGAG 1 cut(s) 29
HaeIII GGCC 3 cut(s) 23, 138, 461
Hin1II CATG 1 cut(s) 405
HindIII AAGCTT 1 cut(s) 376
HinfI GANTC 2 cut(s) 4, 347
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 2 cut(s) 145, 301
Hpy188I TCNGA 2 cut(s) 253, 500
Hpy8I GTNNAC 2 cut(s) 145, 301
HpyAV CCTTC 2 cut(s) 181, 513
HpyCH4III ACNGT 1 cut(s) 149
HpyCH4V TGCA 5 cut(s) 222, 341, 401, 405, 471
HpyF10VI GCNNNNNNNGC 2 cut(s) 375, 477
HpyF3I CTNAG 1 cut(s) 250
Hsp92II CATG 1 cut(s) 405
Kzo9I GATC 2 cut(s) 123, 330
Lsp1109I GCAGC 2 cut(s) 89, 92
LweI GCATC 2 cut(s) 231, 480
MabI ACCWGGT 1 cut(s) 196
MaeIII GTNAC 4 cut(s) 88, 113, 149, 416
MalI GATC 2 cut(s) 125, 332
MboI GATC 2 cut(s) 123, 330
MboII GAAGA 6 cut(s) 28, 31, 217, 354, 384, 387
MhlI GDGCHC 1 cut(s) 258
MlsI TGGCCA 1 cut(s) 23
MluCI AATT 2 cut(s) 493, 521
MluNI TGGCCA 1 cut(s) 23
MmeI TCCRAC 1 cut(s) 288
MnlI CCTC 5 cut(s) 53, 59, 259, 319, 337
Mox20I TGGCCA 1 cut(s) 23
MscI TGGCCA 1 cut(s) 23
MseI TTAA 1 cut(s) 519
MslI CAYNNNNRTG 1 cut(s) 131
Msp20I TGGCCA 1 cut(s) 23
MspR9I CCNGG 3 cut(s) 198, 371, 414
MvaI CCWGG 3 cut(s) 198, 371, 414
MwoI GCNNNNNNNGC 2 cut(s) 375, 477
NdeII GATC 2 cut(s) 123, 330
NlaIII CATG 1 cut(s) 405
NmuCI GTSAC 2 cut(s) 88, 149
NspI RCATGY 1 cut(s) 405
PaeI GCATGC 1 cut(s) 405
PfeI GAWTC 2 cut(s) 4, 347
PkrI GCNGC 2 cut(s) 79, 82
Psp6I CCWGG 3 cut(s) 196, 369, 412
PspGI CCWGG 3 cut(s) 196, 369, 412
PspPI GGNCC 1 cut(s) 136
PstNI CAGNNNCTG 1 cut(s) 203
RsaI GTAC 1 cut(s) 302
RsaNI GTAC 1 cut(s) 301
RseI CAYNNNNRTG 1 cut(s) 131
SaqAI TTAA 1 cut(s) 519
SatI GCNGC 2 cut(s) 78, 81
Sau3AI GATC 2 cut(s) 123, 330
Sau96I GGNCC 1 cut(s) 136
ScrFI CCNGG 3 cut(s) 198, 371, 414
SduI GDGCHC 1 cut(s) 258
SetI ASST 7 cut(s) 115, 173, 188, 202, 251, 380, 505
SexAI ACCWGGT 1 cut(s) 196
SfaNI GCATC 2 cut(s) 231, 480
SfcI CTRYAG 1 cut(s) 475
SmiMI CAYNNNNRTG 1 cut(s) 131
SphI GCATGC 1 cut(s) 405
Sse9I AATT 2 cut(s) 493, 521
SsiI CCGC 1 cut(s) 233
StyD4I CCNGG 3 cut(s) 196, 369, 412
TaaI ACNGT 1 cut(s) 149
TaqI TCGA 2 cut(s) 216, 452
TasI AATT 2 cut(s) 493, 521
TatI WGTACW 1 cut(s) 300
TfiI GAWTC 2 cut(s) 4, 347
Tru1I TTAA 1 cut(s) 519
Tru9I TTAA 1 cut(s) 519
TseFI GTSAC 2 cut(s) 88, 149
TseI GCWGC 2 cut(s) 77, 80
Tsp45I GTSAC 2 cut(s) 88, 149
TspDTI ATGAA 3 cut(s) 17, 18, 450
XapI RAATTY 1 cut(s) 493
XceI RCATGY 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.