MD17G1016900.v1.1

lysine histidine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008192
Physical Location & Seq
Reverse (-)
1340853 .. 1343795
2943 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1016900.v1.1.491

Sequence Viewer

Length: 1380 bp
ATGGGAGATACAACCGACCAAAGTTCTTCTGTCCCAAGCAATGTGGACGAGAAATGGAACAAGCAATCACAGATTATTGGTGAAGATAAGCTACGGAGGCAGAAGGAAATAGATGAGTGGCTTCCAATCACTGGTTCAAGGAATGCAAAATGGTGGTACTCAACTTTCCACAATGTCACCGCCATGGTTGGAGCCGGCGTCTTAAGTCTTCCCTTTGCCATGTCCAATCTCGGATGGGGTCCTGGTTGTGTGATTCTTGTACTATCTTGGGTGATCACTCTATTCACTTTATGGCAAATGGTTGAGATGCATGAGATGGTTCCCGGAAAGAGGTTTGATAGGTACCATGAGCTAGGGCAACATGCCTTTGGTGAAAAGCTAGGGCTTTGGATTGTGGTGCCCCAACAGCTCATATGTGAAGTTGGTGTGGACATTGTGTATATGGTCACGGGGGGAAACTCGCTGCAGAAAGTTTACAAGACGGTGAAGAAAGACAAGGATCCGATCAAACTCACCTACTTCATAATGATCTTTGCCTCCTGTCATTTTGTCCTCGCCCATCTCCCCAACTTCAACTCCATCTCCGGCGTTTCGTTAGCTGCAGCAGTCATGTCATTAAGTTACTCTACTATTGCATGGACTGCTTCCCTCCACAAGGGTGTTCAGCCGGATGTAGCATACGGGTATAAAGCCAGGAGCACTCCAGGAACAGTATTCAACTTCTTCACTGCCTTGGGGGACGTAGCTTTTGCCTATGCAGGCCACAATGTGGTTTTGGAGATCCAAGCAACAATCCCTTCGACGCCCGAAAAGCCATCGAAGAAGCCTATGTGGAGAGGAGTTGTGTTCGCCTACCTCGTCGTGGCCTTGTGCTACTTTCCAGTGGCTCTCATTGGATATTACACTTTTGGAAACGCAGTGGAGGACAATATCCTCATCTCGTTAGAGAAACCTGAGTGGCTCATCATAACTGCAAACATGTTTGTTGTCGTCCATGTCATTGGAAGTTATCAGCTATATGCAATGCCAGTATTTGACATGATAGAGACCTTGTTGGTGAAGAAACTGCACTTCAGGCCTACCCTAATCCTCCGCTTTGTTTCCCGGAATATCTATGTTGCCTTCACAATGTTTGTCGCCATTACTTTCCCCTTCTTCGGTGGTCTTCTAGGATTCTTTGGAGGATTTGCATTTTCCCCGACAACATACTTTCTCCCTTGCGTTCTGTGGCTTGCCATCAAGAAACCGCGCAGGTGGAGCCTATCTTGGATTATTAACTGGATTTGCATTACTCTCGGCGTCATGCTGATGACTCTATCACCTATTGGAGGGTTGAGGAGTATCATAATTCAAGCCAGTACCTACAAATTCTACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

460

Amino Acids

51.69

Weight (kDa)

9.15

Isoelectric Point (pI)

44.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 48 - 443 7.1e-111 Transmembrane amino acid transporter protein
Trp_Tyr_perm PF03222 54 - 187 1.9e-09 Tryptophan/tyrosine permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1242
Acc36I ACCTGC 1 cut(s) 1242
Acc65I GGTACC 1 cut(s) 342
AccB1I GGYRCC 2 cut(s) 342, 397
AccB7I CCANNNNNTGG 2 cut(s) 131, 769
AccII CGCG 1 cut(s) 1249
AciI CCGC 3 cut(s) 180, 1093, 1247
AclWI GGATC 3 cut(s) 494, 507, 775
AcsI RAATTY 1 cut(s) 1367
AcuI CTGAAG 1 cut(s) 1057
AcyI GRCGYC 3 cut(s) 198, 803, 1299
AdeI CACNNNGTG 1 cut(s) 769
AfaI GTAC 4 cut(s) 158, 261, 344, 1360
AfiI CCNNNNNNNGG 7 cut(s) 131, 330, 655, 769, 862, 1157, 1328
AflII CTTAAG 1 cut(s) 202
AflIII ACRYGT 1 cut(s) 978
AgsI TTSAA 4 cut(s) 138, 574, 718, 1352
AjnI CCWGG 3 cut(s) 241, 692, 703
AjuI GAANNNNNNNTTGG 2 cut(s) 118, 150
AleI CACNNNNGTG 1 cut(s) 657
AluBI AGCT 7 cut(s) 91, 352, 379, 409, 599, 746, 1015
AluI AGCT 7 cut(s) 91, 352, 379, 409, 599, 746, 1015
Alw21I GWGCWC 1 cut(s) 701
Alw26I GTCTC 1 cut(s) 1040
AlwI GGATC 3 cut(s) 494, 507, 775
AoxI GGCC 3 cut(s) 760, 864, 1076
ApeKI GCWGC 3 cut(s) 463, 599, 602
ApoI RAATTY 1 cut(s) 1367
ArsI GACNNNNNNTTYG 2 cut(s) 731, 763
Asp718I GGTACC 1 cut(s) 342
AspLEI GCGC 1 cut(s) 1251
AspS9I GGNCC 1 cut(s) 239
AsuC2I CCSGG 2 cut(s) 324, 1105
AsuHPI GGTGA 8 cut(s) 92, 169, 283, 383, 496, 505, 1069, 1311
AvaII GGWCC 1 cut(s) 239
BaeGI GKGCMC 1 cut(s) 402
BamHI GGATCC 1 cut(s) 499
BanI GGYRCC 2 cut(s) 342, 397
BarI GAAGNNNNNNTAC 2 cut(s) 75, 107
BbsI GAAGAC 2 cut(s) 200, 1157
Bbv12I GWGCWC 1 cut(s) 701
BbvI GCAGC 3 cut(s) 450, 586, 614
BccI CCATC 6 cut(s) 228, 310, 567, 587, 823, 1244
BcgI CGANNNNNNTGC 2 cut(s) 1276, 1310
BciT130I CCWGG 3 cut(s) 243, 694, 705
BclI TGATCA 1 cut(s) 273
BcnI CCSGG 2 cut(s) 324, 1105
BcoDI GTCTC 1 cut(s) 1040
BfaI CTAG 3 cut(s) 353, 380, 1169
BfmI CTRYAG 2 cut(s) 464, 600
BfrI CTTAAG 1 cut(s) 202
BfuAI ACCTGC 1 cut(s) 1242
BisI GCNGC 3 cut(s) 464, 600, 603
BlsI GCNGC 3 cut(s) 465, 601, 604
Bme1390I CCNGG 5 cut(s) 243, 324, 694, 705, 1105
Bme18I GGWCC 1 cut(s) 239
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 7 cut(s) 193, 240, 321, 344, 399, 501, 1259
BmrFI CCNGG 5 cut(s) 243, 324, 694, 705, 1105
BmsI GCATC 1 cut(s) 297
BpiI GAAGAC 2 cut(s) 200, 1157
BpmI CTGGAG 1 cut(s) 687
BpuMI CCSGG 2 cut(s) 324, 1105
BsaHI GRCGYC 3 cut(s) 198, 803, 1299
BsaI GGTCTC 1 cut(s) 1040
BsaJI CCNNGG 2 cut(s) 183, 732
BsaXI ACNNNNNCTCC 6 cut(s) 183, 213, 560, 566, 590, 596
Bsc4I CCNNNNNNNGG 7 cut(s) 131, 330, 655, 769, 862, 1157, 1328
Bse118I RCCGGY 1 cut(s) 194
Bse1I ACTGG 5 cut(s) 136, 881, 1028, 1283, 1356
Bse3DI GCAATG 2 cut(s) 46, 1029
BseBI CCWGG 3 cut(s) 243, 694, 705
BseDI CCNNGG 2 cut(s) 183, 732
BseGI GGATG 2 cut(s) 239, 676
BseLI CCNNNNNNNGG 7 cut(s) 131, 330, 655, 769, 862, 1157, 1328
BseMI GCAATG 2 cut(s) 46, 1029
BseMII CTCAG 1 cut(s) 945
BseNI ACTGG 5 cut(s) 136, 881, 1028, 1283, 1356
BseRI GAGGAG 2 cut(s) 852, 1351
BseSI GKGCMC 1 cut(s) 402
BseXI GCAGC 3 cut(s) 450, 586, 614
BsgI GTGCAG 1 cut(s) 1052
Bsh1236I CGCG 1 cut(s) 1249
BshFI GGCC 3 cut(s) 762, 866, 1078
BshNI GGYRCC 2 cut(s) 342, 397
BsiHKAI GWGCWC 1 cut(s) 701
BsiSI CCGG 5 cut(s) 195, 324, 585, 668, 1105
BslFI GGGAC 2 cut(s) 17, 752
BslI CCNNNNNNNGG 7 cut(s) 131, 330, 655, 769, 862, 1157, 1328
BsmAI GTCTC 1 cut(s) 1040
BsmFI GGGAC 2 cut(s) 17, 752
BsmI GAATGC 1 cut(s) 148
BsnI GGCC 3 cut(s) 762, 866, 1078
Bso31I GGTCTC 1 cut(s) 1040
Bsp1286I GDGCHC 2 cut(s) 402, 701
Bsp143I GATC 5 cut(s) 273, 499, 504, 528, 780
Bsp19I CCATGG 1 cut(s) 183
BspACI CCGC 3 cut(s) 180, 1093, 1247
BspANI GGCC 3 cut(s) 762, 866, 1078
BspCNI CTCAG 1 cut(s) 946
BspFNI CGCG 1 cut(s) 1249
BspLI GGNNCC 7 cut(s) 193, 240, 321, 344, 399, 501, 1259
BspMAI CTGCAG 2 cut(s) 468, 604
BspMI ACCTGC 1 cut(s) 1242
BspPI GGATC 3 cut(s) 494, 507, 775
BspT107I GGYRCC 2 cut(s) 342, 397
BspTI CTTAAG 1 cut(s) 202
BspTNI GGTCTC 1 cut(s) 1040
BsrDI GCAATG 2 cut(s) 46, 1029
BsrFI RCCGGY 1 cut(s) 194
BsrI ACTGG 5 cut(s) 136, 881, 1028, 1283, 1356
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 2 cut(s) 183, 732
BssMI GATC 5 cut(s) 273, 499, 504, 528, 780
BssNI GRCGYC 3 cut(s) 198, 803, 1299
BssT1I CCWWGG 2 cut(s) 183, 732
Bst2UI CCWGG 3 cut(s) 243, 694, 705
Bst4CI ACNGT 2 cut(s) 484, 712
BstACI GRCGYC 3 cut(s) 198, 803, 1299
BstAFI CTTAAG 1 cut(s) 202
BstAPI GCANNNNNTGC 1 cut(s) 641
BstC8I GCNNGC 3 cut(s) 196, 760, 1233
BstDEI CTNAG 1 cut(s) 954
BstDSI CCRYGG 1 cut(s) 183
BstENI CCTNNNNNAGG 2 cut(s) 653, 1326
BstF5I GGATG 2 cut(s) 239, 676
BstFNI CGCG 1 cut(s) 1249
BstHHI GCGC 1 cut(s) 1251
BstKTI GATC 5 cut(s) 276, 502, 507, 531, 783
BstMAI GTCTC 1 cut(s) 1040
BstMBI GATC 5 cut(s) 273, 499, 504, 528, 780
BstMWI GCNNNNNNNGC 6 cut(s) 97, 406, 641, 811, 1075, 1257
BstNI CCWGG 3 cut(s) 243, 694, 705
BstNSI RCATGY 2 cut(s) 365, 982
BstSCI CCNGG 5 cut(s) 241, 322, 692, 703, 1103
BstSFI CTRYAG 2 cut(s) 464, 600
BstSLI GKGCMC 1 cut(s) 402
BstUI CGCG 1 cut(s) 1249
BstV1I GCAGC 3 cut(s) 450, 586, 614
BstV2I GAAGAC 2 cut(s) 200, 1157
BstX2I RGATCY 2 cut(s) 499, 780
BstXI CCANNNNNNTGG 1 cut(s) 1001
BstYI RGATCY 2 cut(s) 499, 780
BsuRI GGCC 3 cut(s) 762, 866, 1078
BtgI CCRYGG 1 cut(s) 183
BtsCI GGATG 2 cut(s) 239, 676
BtsI GCAGTG 2 cut(s) 726, 924
BtsIMutI CAGTG 4 cut(s) 129, 726, 888, 924
BveI ACCTGC 1 cut(s) 1242
Cac8I GCNNGC 3 cut(s) 196, 760, 1233
CfoI GCGC 1 cut(s) 1251
Cfr10I RCCGGY 1 cut(s) 194
Cfr13I GGNCC 1 cut(s) 239
CseI GACGC 3 cut(s) 187, 811, 1288
Csp6I GTAC 4 cut(s) 157, 260, 343, 1359
CspCI CAANNNNNGTGG 2 cut(s) 24, 59
CviQI GTAC 4 cut(s) 157, 260, 343, 1359
DdeI CTNAG 1 cut(s) 954
DpnI GATC 5 cut(s) 275, 501, 506, 530, 782
DpnII GATC 5 cut(s) 273, 499, 504, 528, 780
DraIII CACNNNGTG 1 cut(s) 769
Eco130I CCWWGG 2 cut(s) 183, 732
Eco147I AGGCCT 1 cut(s) 1078
Eco31I GGTCTC 1 cut(s) 1040
Eco47I GGWCC 1 cut(s) 239
Eco57I CTGAAG 1 cut(s) 1057
EcoNI CCTNNNNNAGG 2 cut(s) 653, 1326
EcoO109I RGGNCCY 1 cut(s) 239
EcoRII CCWGG 3 cut(s) 241, 692, 703
EcoT14I CCWWGG 2 cut(s) 183, 732
EcoT22I ATGCAT 1 cut(s) 312
ErhI CCWWGG 2 cut(s) 183, 732
FaqI GGGAC 2 cut(s) 17, 752
FauNDI CATATG 1 cut(s) 413
FbaI TGATCA 1 cut(s) 273
Fnu4HI GCNGC 3 cut(s) 464, 600, 603
FokI GGATG 2 cut(s) 246, 683
Fsp4HI GCNGC 3 cut(s) 464, 600, 603
FspBI CTAG 3 cut(s) 353, 380, 1169
GlaI GCGC 1 cut(s) 1250
GluI GCNGC 3 cut(s) 464, 600, 603
GsuI CTGGAG 1 cut(s) 687
HaeIII GGCC 3 cut(s) 762, 866, 1078
HapII CCGG 5 cut(s) 195, 324, 585, 668, 1105
HgaI GACGC 3 cut(s) 187, 811, 1288
HhaI GCGC 1 cut(s) 1251
Hin1I GRCGYC 3 cut(s) 198, 803, 1299
Hin6I GCGC 1 cut(s) 1249
HinP1I GCGC 1 cut(s) 1249
HinfI GANTC 3 cut(s) 253, 1173, 1312
HpaII CCGG 5 cut(s) 195, 324, 585, 668, 1105
HphI GGTGA 8 cut(s) 92, 169, 283, 383, 496, 505, 1069, 1311
Hpy166II GTNNAC 3 cut(s) 46, 430, 475
Hpy188I TCNGA 2 cut(s) 233, 504
Hpy188III TCNNGA 1 cut(s) 1240
Hpy8I GTNNAC 3 cut(s) 46, 430, 475
Hpy99I CGWCG 2 cut(s) 805, 863
HpyAV CCTTC 4 cut(s) 97, 807, 1132, 1162
HpyCH4III ACNGT 2 cut(s) 484, 712
HpyCH4IV ACGT 1 cut(s) 741
HpyF10VI GCNNNNNNNGC 6 cut(s) 97, 406, 641, 811, 1075, 1257
HpyF3I CTNAG 1 cut(s) 954
HpySE526I ACGT 1 cut(s) 741
Hsp92I GRCGYC 3 cut(s) 198, 803, 1299
HspAI GCGC 1 cut(s) 1249
KpnI GGTACC 1 cut(s) 346
KroI GCCGGC 1 cut(s) 194
KroNI GCCGGC 1 cut(s) 196
Ksp22I TGATCA 1 cut(s) 273
Kzo9I GATC 5 cut(s) 273, 499, 504, 528, 780
LmnI GCTCC 3 cut(s) 191, 696, 1257
Lsp1109I GCAGC 3 cut(s) 450, 586, 614
LweI GCATC 1 cut(s) 297
MaeI CTAG 3 cut(s) 353, 380, 1169
MaeII ACGT 1 cut(s) 741
MaeIII GTNAC 3 cut(s) 175, 445, 620
MalI GATC 5 cut(s) 275, 501, 506, 530, 782
MboI GATC 5 cut(s) 273, 499, 504, 528, 780
MboII GAAGA 9 cut(s) 18, 95, 200, 499, 715, 832, 1072, 1147, 1157
MflI RGATCY 2 cut(s) 499, 780
MhlI GDGCHC 2 cut(s) 402, 701
MluCI AATT 2 cut(s) 1347, 1367
MlyI GAGTC 1 cut(s) 1306
MmeI TCCRAC 1 cut(s) 169
Mph1103I ATGCAT 1 cut(s) 312
MroNI GCCGGC 1 cut(s) 194
MseI TTAA 3 cut(s) 203, 617, 1275
MslI CAYNNNNRTG 3 cut(s) 182, 657, 1307
MspCI CTTAAG 1 cut(s) 202
MspI CCGG 5 cut(s) 195, 324, 585, 668, 1105
MspR9I CCNGG 5 cut(s) 243, 324, 694, 705, 1105
Mva1269I GAATGC 1 cut(s) 148
MvaI CCWGG 3 cut(s) 243, 694, 705
MvnI CGCG 1 cut(s) 1249
MwoI GCNNNNNNNGC 6 cut(s) 97, 406, 641, 811, 1075, 1257
NaeI GCCGGC 1 cut(s) 196
NciI CCSGG 2 cut(s) 324, 1105
NcoI CCATGG 1 cut(s) 183
NdeI CATATG 1 cut(s) 413
NdeII GATC 5 cut(s) 273, 499, 504, 528, 780
NgoMIV GCCGGC 1 cut(s) 194
NlaIV GGNNCC 7 cut(s) 193, 240, 321, 344, 399, 501, 1259
NmeAIII GCCGAG 1 cut(s) 1275
NmuCI GTSAC 2 cut(s) 175, 445
NsiI ATGCAT 1 cut(s) 312
NspI RCATGY 2 cut(s) 365, 982
OliI CACNNNNGTG 1 cut(s) 657
PaqCI CACCTGC 1 cut(s) 1242
PceI AGGCCT 1 cut(s) 1078
PciI ACATGT 1 cut(s) 978
PcsI WCGNNNNNNNCGW 1 cut(s) 855
PctI GAATGC 1 cut(s) 148
PdiI GCCGGC 1 cut(s) 196
PfeI GAWTC 2 cut(s) 253, 1173
PflMI CCANNNNNTGG 2 cut(s) 131, 769
PfoI TCCNGGA 3 cut(s) 322, 703, 1103
PkrI GCNGC 3 cut(s) 465, 601, 604
PleI GAGTC 1 cut(s) 1306
PpsI GAGTC 1 cut(s) 1306
PpuMI RGGWCCY 1 cut(s) 239
PscI ACATGT 1 cut(s) 978
Psp5II RGGWCCY 1 cut(s) 239
Psp6I CCWGG 3 cut(s) 241, 692, 703
PspGI CCWGG 3 cut(s) 241, 692, 703
PspN4I GGNNCC 7 cut(s) 193, 240, 321, 344, 399, 501, 1259
PspPI GGNCC 1 cut(s) 239
PspPPI RGGWCCY 1 cut(s) 239
PstI CTGCAG 2 cut(s) 468, 604
PsuI RGATCY 2 cut(s) 499, 780
RsaI GTAC 4 cut(s) 158, 261, 344, 1360
RsaNI GTAC 4 cut(s) 157, 260, 343, 1359
RseI CAYNNNNRTG 3 cut(s) 182, 657, 1307
SaqAI TTAA 3 cut(s) 203, 617, 1275
SatI GCNGC 3 cut(s) 464, 600, 603
Sau3AI GATC 5 cut(s) 273, 499, 504, 528, 780
Sau96I GGNCC 1 cut(s) 239
SchI GAGTC 1 cut(s) 1306
ScrFI CCNGG 5 cut(s) 243, 324, 694, 705, 1105
SduI GDGCHC 2 cut(s) 402, 701
SfaNI GCATC 1 cut(s) 297
SfcI CTRYAG 2 cut(s) 464, 600
SinI GGWCC 1 cut(s) 239
SmiMI CAYNNNNRTG 3 cut(s) 182, 657, 1307
SmlI CTYRAG 1 cut(s) 202
SmoI CTYRAG 1 cut(s) 202
Sse9I AATT 2 cut(s) 1347, 1367
SseBI AGGCCT 1 cut(s) 1078
SsiI CCGC 3 cut(s) 180, 1093, 1247
SspMI CTAG 3 cut(s) 353, 380, 1169
StuI AGGCCT 1 cut(s) 1078
StyD4I CCNGG 5 cut(s) 241, 322, 692, 703, 1103
StyI CCWWGG 2 cut(s) 183, 732
TaaI ACNGT 2 cut(s) 484, 712
TaiI ACGT 1 cut(s) 744
TaqI TCGA 2 cut(s) 800, 818
TasI AATT 2 cut(s) 1347, 1367
TatI WGTACW 1 cut(s) 259
TfiI GAWTC 2 cut(s) 253, 1173
Tru1I TTAA 3 cut(s) 203, 617, 1275
Tru9I TTAA 3 cut(s) 203, 617, 1275
TscAI CASTG 4 cut(s) 136, 733, 888, 924
TseFI GTSAC 2 cut(s) 175, 445
TseI GCWGC 3 cut(s) 463, 599, 602
Tsp45I GTSAC 2 cut(s) 175, 445
TspDTI ATGAA 1 cut(s) 511
TspGWI ACGGA 1 cut(s) 109
TspRI CASTG 4 cut(s) 136, 733, 888, 924
Van91I CCANNNNNTGG 2 cut(s) 131, 769
Vha464I CTTAAG 1 cut(s) 202
VpaK11BI GGWCC 1 cut(s) 239
XagI CCTNNNNNAGG 2 cut(s) 653, 1326
XapI RAATTY 1 cut(s) 1367
XceI RCATGY 2 cut(s) 365, 982
XspI CTAG 3 cut(s) 353, 380, 1169
Zsp2I ATGCAT 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.