pycom111g01320

Lysine histidine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0008192
Physical Location & Seq
Reverse (-)
843250 .. 845320
2071 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g01320.8

Sequence Viewer

Length: 1386 bp
ATGGTAGCTCAAGCTCCGACTGACGGAAATTCTTCCAACCCCAACAATGTGGACGAGACACCGGAGATGCGGCGGCAGATCAGTGATGATGAAAAGGTAAGGAAGCAGAAGGAAATAAATGATTGGCTTCCAATTACTTCATCAAGGAATGCAAATTGGTGGTACGCAGCTTTCCACAATGTCAATTCCATGGTTGGAGCTGGTGTCCTAGGTCTTCCCTTTGCAATGTCCAATCTCGGATGGGGTCCAGGATGTGTTGTAATGGTGCTTTCATGGGTGATCACCTTGTTCACGTTGTGGCAAATGGTTGAGATGCATGAAATGGTTCCCGGCAAGCGGTTCGATAGGTACCATGAGCTAGGGCAACACGCCTTCGGCGAAAAGCTTGGCCTCTGGATTGTGGTGCCTCAGCAGCTCGTATGCGAAGTTGGTGTTAACATTTTGTACATGGTCACAGGTGGACAATCCCTGCAGAAGATCCATAAGATAGTGAAGAAAGACAAGGATCCCATCAAGCTCACCTACTTCATCATGATCTTTGCCTCCGTGGAATTTGTCCTCTCCCATCTCCCCAACTTCAACTCCATCTCCGGCATCTCATTAGCCGCGGCGGTGATGTCACTAAGTTACTCTACCATAGCATGGACTACTTCAATCCACAAGGGTGTTCAGCCAGACGTAGAGTACGGGTACAAAGCTCGAAGCACGTCGGGGACAGTCTTCAACTTCTTCAACGCCTTGGGGCAGGTAGCTTTTGCCTATGCAGGCCACAACGTGGTTTTGGAGATCCAAGCTACACTCCCCACTTCTCCAGAAAAGCCATCGAAGTATGCTATGTGGAGAGGGGTGGTGATCGCCTACATAGTGGTGGCCTTGTGCTATTTTCCGGTGGCTTTCATCGGATATTACACTTTTGGGAACACGGTGGAGGACAACATCCTAATCTCGTTAGAGAAACCCGGATGGCTCATTGTAACAGCAAACATGTTTGTTATCATCCATATCATTGGGGGTTATCAGCTATTTGCAATGCCAGTATTTGACATGATAGAAACTGTGTTGGTGAAGAAACTGCATTTTCGCCCCACAACGAAGCTTCGCTTTATTACTAGGAACATATACGTTGCGTTCACAATGGTCGTCGGAATGACCTTTCCCTTCTTCAGTGGTATCATGGGATTCCTCGGAGGATTTGCTTATTCCCCAACATCATACTATCTCCCGTGCATTATATGGCTAGCCATCAAGAAACCGCGCAAGTTTAGCCTATCGTGGTTTATTAACTGGATTTGTATCATACTCGGCGTCCTGCTGATGATCGTCTCGCCTATCGGAGGTTTCCGGAATATCATACTTCAGGCCAAGGAATACCACTTCTACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

462

Amino Acids

52.01

Weight (kDa)

9.05

Isoelectric Point (pI)

43.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 736
Acc65I GGTACC 1 cut(s) 348
AccB1I GGYRCC 2 cut(s) 348, 403
AccB7I CCANNNNNTGG 2 cut(s) 642, 775
AccII CGCG 2 cut(s) 608, 1255
AccIII TCCGGA 1 cut(s) 1341
AciI CCGC 7 cut(s) 70, 73, 337, 606, 608, 611, 1253
AclWI GGATC 4 cut(s) 472, 500, 513, 781
AcsI RAATTY 2 cut(s) 28, 551
AcuI CTGAAG 2 cut(s) 1147, 1340
AcyI GRCGYC 1 cut(s) 1305
AdeI CACNNNGTG 2 cut(s) 297, 775
AfaI GTAC 5 cut(s) 164, 350, 446, 686, 692
AfiI CCNNNNNNNGG 5 cut(s) 23, 336, 642, 775, 1334
AflIII ACRYGT 1 cut(s) 984
AgsI TTSAA 4 cut(s) 580, 654, 724, 733
AjiI CACGTC 1 cut(s) 708
AjnI CCWGG 1 cut(s) 247
AleI CACNNNNGTG 1 cut(s) 663
Alw26I GTCTC 2 cut(s) 50, 1327
AlwI GGATC 4 cut(s) 472, 500, 513, 781
Aor13HI TCCGGA 1 cut(s) 1341
AoxI GGCC 4 cut(s) 388, 766, 870, 1359
ApeKI GCWGC 2 cut(s) 167, 412
ApoI RAATTY 2 cut(s) 28, 551
Asp700I GAANNNNTTC 2 cut(s) 31, 324
Asp718I GGTACC 1 cut(s) 348
AspA2I CCTAGG 1 cut(s) 208
AspLEI GCGC 1 cut(s) 1257
AspS9I GGNCC 1 cut(s) 245
AsuC2I CCSGG 2 cut(s) 330, 960
AsuHPI GGTGA 6 cut(s) 274, 289, 511, 625, 862, 1075
AsuNHI GCTAGC 1 cut(s) 1237
AvaII GGWCC 1 cut(s) 245
AvrII CCTAGG 1 cut(s) 208
BamHI GGATCC 1 cut(s) 505
BanI GGYRCC 2 cut(s) 348, 403
BbsI GAAGAC 2 cut(s) 206, 712
BbvCI CCTCAGC 1 cut(s) 408
BbvI GCAGC 2 cut(s) 179, 424
BccI CCATC 7 cut(s) 234, 518, 573, 593, 829, 957, 1250
BcgI CGANNNNNNTGC 2 cut(s) 322, 356
BciT130I CCWGG 1 cut(s) 249
BclI TGATCA 1 cut(s) 279
BcnI CCSGG 2 cut(s) 330, 960
BcoDI GTCTC 2 cut(s) 50, 1327
BfaI CTAG 4 cut(s) 209, 359, 1110, 1238
BfmI CTRYAG 1 cut(s) 470
BfuAI ACCTGC 1 cut(s) 736
BisI GCNGC 6 cut(s) 71, 74, 168, 413, 606, 609
BlnI CCTAGG 1 cut(s) 208
BlsI GCNGC 6 cut(s) 72, 75, 169, 414, 607, 610
Bme1390I CCNGG 3 cut(s) 249, 330, 960
Bme18I GGWCC 1 cut(s) 245
BmgBI CACGTC 1 cut(s) 708
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 5 cut(s) 246, 327, 350, 405, 507
BmrFI CCNGG 3 cut(s) 249, 330, 960
BmsI GCATC 3 cut(s) 57, 303, 603
BmtI GCTAGC 1 cut(s) 1241
BpiI GAAGAC 2 cut(s) 206, 712
BpmI CTGGAG 1 cut(s) 795
Bpu10I CCTNAGC 1 cut(s) 408
BpuMI CCSGG 2 cut(s) 330, 960
BsaBI GATNNNNATC 1 cut(s) 1292
BsaHI GRCGYC 1 cut(s) 1305
BsaJI CCNNGG 7 cut(s) 189, 208, 546, 606, 738, 1183, 1362
BsaWI WCCGGW 3 cut(s) 61, 886, 1341
BsaXI ACNNNNNCTCC 6 cut(s) 189, 219, 566, 572, 596, 602
Bsc4I CCNNNNNNNGG 5 cut(s) 23, 336, 642, 775, 1334
Bse1I ACTGG 2 cut(s) 1034, 1289
Bse3DI GCAATG 2 cut(s) 231, 1035
Bse8I GATNNNNATC 1 cut(s) 1292
BseAI TCCGGA 1 cut(s) 1341
BseBI CCWGG 1 cut(s) 249
BseDI CCNNGG 7 cut(s) 189, 208, 546, 606, 738, 1183, 1362
BseGI GGATG 5 cut(s) 245, 257, 936, 968, 996
BseJI GATNNNNATC 1 cut(s) 1292
BseLI CCNNNNNNNGG 5 cut(s) 23, 336, 642, 775, 1334
BseMI GCAATG 2 cut(s) 231, 1035
BseMII CTCAG 1 cut(s) 422
BseNI ACTGG 2 cut(s) 1034, 1289
BseXI GCAGC 2 cut(s) 179, 424
Bsh1236I CGCG 2 cut(s) 608, 1255
BshFI GGCC 4 cut(s) 390, 768, 872, 1361
BshNI GGYRCC 2 cut(s) 348, 403
BsiSI CCGG 6 cut(s) 62, 330, 591, 887, 960, 1342
BslFI GGGAC 1 cut(s) 727
BslI CCNNNNNNNGG 5 cut(s) 23, 336, 642, 775, 1334
BsmAI GTCTC 2 cut(s) 50, 1327
BsmBI CGTCTC 1 cut(s) 1327
BsmFI GGGAC 1 cut(s) 727
BsmI GAATGC 1 cut(s) 154
BsnI GGCC 4 cut(s) 390, 768, 872, 1361
Bsp13I TCCGGA 1 cut(s) 1341
Bsp1407I TGTACA 1 cut(s) 444
Bsp143I GATC 8 cut(s) 78, 279, 477, 505, 534, 786, 852, 1317
Bsp19I CCATGG 1 cut(s) 189
BspACI CCGC 7 cut(s) 70, 73, 337, 606, 608, 611, 1253
BspANI GGCC 4 cut(s) 390, 768, 872, 1361
BspCNI CTCAG 1 cut(s) 421
BspEI TCCGGA 1 cut(s) 1341
BspFNI CGCG 2 cut(s) 608, 1255
BspHI TCATGA 1 cut(s) 531
BspLI GGNNCC 5 cut(s) 246, 327, 350, 405, 507
BspMAI CTGCAG 1 cut(s) 474
BspMI ACCTGC 1 cut(s) 736
BspOI GCTAGC 1 cut(s) 1241
BspPI GGATC 4 cut(s) 472, 500, 513, 781
BspT107I GGYRCC 2 cut(s) 348, 403
BsrDI GCAATG 2 cut(s) 231, 1035
BsrGI TGTACA 1 cut(s) 444
BsrI ACTGG 2 cut(s) 1034, 1289
BssECI CCNNGG 7 cut(s) 189, 208, 546, 606, 738, 1183, 1362
BssMI GATC 8 cut(s) 78, 279, 477, 505, 534, 786, 852, 1317
BssNI GRCGYC 1 cut(s) 1305
BssT1I CCWWGG 4 cut(s) 189, 208, 738, 1362
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 3 cut(s) 718, 925, 1057
BstACI GRCGYC 1 cut(s) 1305
BstAUI TGTACA 1 cut(s) 444
BstC8I GCNNGC 3 cut(s) 335, 766, 1239
BstDEI CTNAG 2 cut(s) 408, 623
BstDSI CCRYGG 3 cut(s) 189, 546, 606
BstENI CCTNNNNNAGG 1 cut(s) 1332
BstF5I GGATG 5 cut(s) 245, 257, 936, 968, 996
BstFNI CGCG 2 cut(s) 608, 1255
BstHHI GCGC 1 cut(s) 1257
BstKTI GATC 8 cut(s) 81, 282, 480, 508, 537, 789, 855, 1320
BstMAI GTCTC 2 cut(s) 50, 1327
BstMBI GATC 8 cut(s) 78, 279, 477, 505, 534, 786, 852, 1317
BstMWI GCNNNNNNNGC 2 cut(s) 412, 1263
BstNI CCWGG 1 cut(s) 249
BstNSI RCATGY 1 cut(s) 988
BstSCI CCNGG 3 cut(s) 247, 328, 958
BstSFI CTRYAG 1 cut(s) 470
BstUI CGCG 2 cut(s) 608, 1255
BstV1I GCAGC 2 cut(s) 179, 424
BstV2I GAAGAC 2 cut(s) 206, 712
BstX2I RGATCY 3 cut(s) 477, 505, 786
BstXI CCANNNNNNTGG 2 cut(s) 49, 1007
BstYI RGATCY 3 cut(s) 477, 505, 786
BsuRI GGCC 4 cut(s) 390, 768, 872, 1361
BtgI CCRYGG 3 cut(s) 189, 546, 606
BtrI CACGTC 1 cut(s) 708
BtsCI GGATG 5 cut(s) 245, 257, 936, 968, 996
BtsIMutI CAGTG 2 cut(s) 88, 1171
BveI ACCTGC 1 cut(s) 736
Cac8I GCNNGC 3 cut(s) 335, 766, 1239
CciI TCATGA 1 cut(s) 531
CfoI GCGC 1 cut(s) 1257
Cfr13I GGNCC 1 cut(s) 245
Cfr42I CCGCGG 1 cut(s) 609
CseI GACGC 1 cut(s) 1294
Csp6I GTAC 5 cut(s) 163, 349, 445, 685, 691
CviQI GTAC 5 cut(s) 163, 349, 445, 685, 691
DdeI CTNAG 2 cut(s) 408, 623
DpnI GATC 8 cut(s) 80, 281, 479, 507, 536, 788, 854, 1319
DpnII GATC 8 cut(s) 78, 279, 477, 505, 534, 786, 852, 1317
DraIII CACNNNGTG 2 cut(s) 297, 775
Eco130I CCWWGG 4 cut(s) 189, 208, 738, 1362
Eco47I GGWCC 1 cut(s) 245
Eco57I CTGAAG 2 cut(s) 1147, 1340
EcoNI CCTNNNNNAGG 1 cut(s) 1332
EcoRII CCWGG 1 cut(s) 247
EcoT14I CCWWGG 4 cut(s) 189, 208, 738, 1362
EcoT22I ATGCAT 1 cut(s) 318
ErhI CCWWGG 4 cut(s) 189, 208, 738, 1362
Esp3I CGTCTC 1 cut(s) 1327
FalI AAGNNNNNCTT 2 cut(s) 1085, 1117
FaqI GGGAC 1 cut(s) 727
FbaI TGATCA 1 cut(s) 279
Fnu4HI GCNGC 6 cut(s) 71, 74, 168, 413, 606, 609
FokI GGATG 5 cut(s) 252, 264, 923, 975, 983
Fsp4HI GCNGC 6 cut(s) 71, 74, 168, 413, 606, 609
FspBI CTAG 4 cut(s) 209, 359, 1110, 1238
GlaI GCGC 1 cut(s) 1256
GluI GCNGC 6 cut(s) 71, 74, 168, 413, 606, 609
GsuI CTGGAG 1 cut(s) 795
HaeIII GGCC 4 cut(s) 390, 768, 872, 1361
HapII CCGG 6 cut(s) 62, 330, 591, 887, 960, 1342
HgaI GACGC 1 cut(s) 1294
HhaI GCGC 1 cut(s) 1257
Hin1I GRCGYC 1 cut(s) 1305
Hin6I GCGC 1 cut(s) 1255
HinP1I GCGC 1 cut(s) 1255
HincII GTYRAC 1 cut(s) 436
HindII GTYRAC 1 cut(s) 436
HindIII AAGCTT 2 cut(s) 383, 1094
HinfI GANTC 1 cut(s) 1179
HpaI GTTAAC 1 cut(s) 436
HpaII CCGG 6 cut(s) 62, 330, 591, 887, 960, 1342
HphI GGTGA 6 cut(s) 274, 289, 511, 625, 862, 1075
Hpy166II GTNNAC 5 cut(s) 52, 291, 436, 461, 1131
Hpy188I TCNGA 6 cut(s) 18, 239, 902, 1145, 1187, 1334
Hpy188III TCNNGA 5 cut(s) 394, 532, 812, 1246, 1342
Hpy8I GTNNAC 5 cut(s) 52, 291, 436, 461, 1131
Hpy99I CGWCG 2 cut(s) 712, 1145
HpyAV CCTTC 3 cut(s) 103, 382, 1168
HpyCH4III ACNGT 3 cut(s) 718, 925, 1057
HpyCH4IV ACGT 5 cut(s) 293, 678, 707, 774, 1122
HpyCH4V TGCA 8 cut(s) 152, 224, 316, 472, 764, 1028, 1075, 1227
HpyF10VI GCNNNNNNNGC 2 cut(s) 412, 1263
HpyF3I CTNAG 2 cut(s) 408, 623
HpySE526I ACGT 5 cut(s) 293, 678, 707, 774, 1122
Hsp92I GRCGYC 1 cut(s) 1305
HspAI GCGC 1 cut(s) 1255
Kpn2I TCCGGA 1 cut(s) 1341
KpnI GGTACC 1 cut(s) 352
Ksp22I TGATCA 1 cut(s) 279
KspAI GTTAAC 1 cut(s) 436
KspI CCGCGG 1 cut(s) 609
Kzo9I GATC 8 cut(s) 78, 279, 477, 505, 534, 786, 852, 1317
LmnI GCTCC 2 cut(s) 19, 197
Lsp1109I GCAGC 2 cut(s) 179, 424
LweI GCATC 3 cut(s) 57, 303, 603
MaeI CTAG 4 cut(s) 209, 359, 1110, 1238
MaeII ACGT 5 cut(s) 293, 678, 707, 774, 1122
MaeIII GTNAC 4 cut(s) 451, 618, 626, 973
MalI GATC 8 cut(s) 80, 281, 479, 507, 536, 788, 854, 1319
MboI GATC 8 cut(s) 78, 279, 477, 505, 534, 786, 852, 1317
MboII GAAGA 8 cut(s) 24, 206, 487, 505, 712, 721, 1078, 1153
MflI RGATCY 3 cut(s) 477, 505, 786
MluCI AATT 6 cut(s) 28, 132, 154, 184, 551, 1381
MmeI TCCRAC 4 cut(s) 41, 60, 175, 1123
MnlI CCTC 9 cut(s) 401, 417, 553, 569, 836, 922, 1181, 1193, 1328
Mph1103I ATGCAT 1 cut(s) 318
MroI TCCGGA 1 cut(s) 1341
MroXI GAANNNNTTC 2 cut(s) 31, 324
MseI TTAA 3 cut(s) 435, 1281, 1384
MslI CAYNNNNRTG 2 cut(s) 663, 866
MspA1I CMGCKG 1 cut(s) 608
MspI CCGG 6 cut(s) 62, 330, 591, 887, 960, 1342
MspR9I CCNGG 3 cut(s) 249, 330, 960
Mva1269I GAATGC 1 cut(s) 154
MvaI CCWGG 1 cut(s) 249
MvnI CGCG 2 cut(s) 608, 1255
MwoI GCNNNNNNNGC 2 cut(s) 412, 1263
NciI CCSGG 2 cut(s) 330, 960
NcoI CCATGG 1 cut(s) 189
NdeII GATC 8 cut(s) 78, 279, 477, 505, 534, 786, 852, 1317
NheI GCTAGC 1 cut(s) 1237
NlaIV GGNNCC 5 cut(s) 246, 327, 350, 405, 507
NmeAIII GCCGAG 1 cut(s) 1281
NmuCI GTSAC 2 cut(s) 451, 618
NsiI ATGCAT 1 cut(s) 318
NspI RCATGY 1 cut(s) 988
OliI CACNNNNGTG 1 cut(s) 663
PagI TCATGA 1 cut(s) 531
PciI ACATGT 1 cut(s) 984
PcsI WCGNNNNNNNCGW 1 cut(s) 375
PctI GAATGC 1 cut(s) 154
PdmI GAANNNNTTC 2 cut(s) 31, 324
PfeI GAWTC 1 cut(s) 1179
PflMI CCANNNNNTGG 2 cut(s) 642, 775
PfoI TCCNGGA 1 cut(s) 247
PkrI GCNGC 6 cut(s) 72, 75, 169, 414, 607, 610
PscI ACATGT 1 cut(s) 984
Psp6I CCWGG 1 cut(s) 247
PspGI CCWGG 1 cut(s) 247
PspN4I GGNNCC 5 cut(s) 246, 327, 350, 405, 507
PspPI GGNCC 1 cut(s) 245
PstI CTGCAG 1 cut(s) 474
PsuI RGATCY 3 cut(s) 477, 505, 786
RsaI GTAC 5 cut(s) 164, 350, 446, 686, 692
RsaNI GTAC 5 cut(s) 163, 349, 445, 685, 691
RseI CAYNNNNRTG 2 cut(s) 663, 866
SacII CCGCGG 1 cut(s) 609
SaqAI TTAA 3 cut(s) 435, 1281, 1384
SatI GCNGC 6 cut(s) 71, 74, 168, 413, 606, 609
Sau3AI GATC 8 cut(s) 78, 279, 477, 505, 534, 786, 852, 1317
Sau96I GGNCC 1 cut(s) 245
ScrFI CCNGG 3 cut(s) 249, 330, 960
SfaNI GCATC 3 cut(s) 57, 303, 603
SfcI CTRYAG 1 cut(s) 470
Sfr303I CCGCGG 1 cut(s) 609
SgrBI CCGCGG 1 cut(s) 609
SinI GGWCC 1 cut(s) 245
SmiMI CAYNNNNRTG 2 cut(s) 663, 866
SmlI CTYRAG 1 cut(s) 9
SmoI CTYRAG 1 cut(s) 9
Sse9I AATT 6 cut(s) 28, 132, 154, 184, 551, 1381
SsiI CCGC 7 cut(s) 70, 73, 337, 606, 608, 611, 1253
SspMI CTAG 4 cut(s) 209, 359, 1110, 1238
StyD4I CCNGG 3 cut(s) 247, 328, 958
StyI CCWWGG 4 cut(s) 189, 208, 738, 1362
TaaI ACNGT 3 cut(s) 718, 925, 1057
TaiI ACGT 5 cut(s) 296, 681, 710, 777, 1125
TaqI TCGA 3 cut(s) 342, 700, 824
TasI AATT 6 cut(s) 28, 132, 154, 184, 551, 1381
TatI WGTACW 1 cut(s) 444
TauI GCSGC 4 cut(s) 73, 76, 608, 611
TfiI GAWTC 1 cut(s) 1179
Tru1I TTAA 3 cut(s) 435, 1281, 1384
Tru9I TTAA 3 cut(s) 435, 1281, 1384
TscAI CASTG 2 cut(s) 88, 1171
TseFI GTSAC 2 cut(s) 451, 618
TseI GCWGC 2 cut(s) 167, 412
Tsp45I GTSAC 2 cut(s) 451, 618
TspDTI ATGAA 6 cut(s) 105, 129, 261, 333, 517, 886
TspGWI ACGGA 2 cut(s) 39, 535
TspRI CASTG 2 cut(s) 88, 1171
Van91I CCANNNNNTGG 2 cut(s) 642, 775
VpaK11BI GGWCC 1 cut(s) 245
XagI CCTNNNNNAGG 1 cut(s) 1332
XapI RAATTY 2 cut(s) 28, 551
XceI RCATGY 1 cut(s) 988
XmaJI CCTAGG 1 cut(s) 208
XmnI GAANNNNTTC 2 cut(s) 31, 324
XspI CTAG 4 cut(s) 209, 359, 1110, 1238
Zsp2I ATGCAT 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.