Rroxscaffold_2G00078020

lysine histidine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008192
Physical Location & Seq
Reverse (-)
1424690 .. 1427278
2589 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00078020.1

Sequence Viewer

Length: 738 bp
ATGGTTCCGGGAAAGCGGTTTGATAGGTACCATGAACTGGGTCAACATGCCTTTGGTGAAAAGCTTGGTCTTTGGATTGTGGTGCCCCAACAGCTGATCTGCGAAGTTGGCGTGGATATTGTGTACATGGTCACTGGAGGAAAATCGCTGCAGAAGGTCCATAACATAGTTCGTAAAGACGAGAAAGACTCAATCAAACTCAGTTACTTCATCATGATATTTGCCTCTGCTCATTTTGTACTTGCTCATCTCCCCAACTTCAACTCCATATCTGGGGTGTCTTTGGCAGCAGCAGTCATGTCATTAAGTTACTCTACTATTGCATGGACGGCTTCAATCCATAAGGGTGTTCAGGCAGATGTAGATTATGGTTATTTATCCCCCAGCACATCAGGAACAGTCTTTAACTTCTTCAGTGCCTTGGGCGACGTTGCTTTTGCCTATGCAGGCCACAATGTGGTTCTGGAGATTCAAGCAACAATCCCTTCCACACCTGAAACCCCATCGAAGAAGCCTATGTGGAGGGGTGTTGTGATAGCCTACATAGTTGTGGCTTTGTGCTATTTTCCAGTGGCTCTCATTGGATACTACACTTTTGGCAACAAAGTTGAGGACAACATCTTAATATCATTGGAGAAACCTGAATGGCTCATCATAATGGCAAACATGTTTGTTGTTGTCCATGTTATTGGGAGTTATCAGCTGGCCGAATTTCAAGATTCCCCTACAGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

245

Amino Acids

27.09

Weight (kDa)

5.88

Isoelectric Point (pI)

34.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 4 - 236 2.3e-59 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 27
AccB1I GGYRCC 2 cut(s) 27, 82
AccB7I CCANNNNNTGG 2 cut(s) 37, 457
AciI CCGC 1 cut(s) 16
AcoI YGGCCR 1 cut(s) 705
AcsI RAATTY 1 cut(s) 710
AcuI CTGAAG 1 cut(s) 397
AdeI CACNNNGTG 1 cut(s) 457
AfaI GTAC 3 cut(s) 29, 125, 240
AfiI CCNNNNNNNGG 3 cut(s) 37, 273, 457
AflIII ACRYGT 1 cut(s) 666
AgsI TTSAA 4 cut(s) 262, 336, 473, 716
AluBI AGCT 3 cut(s) 64, 94, 703
AluI AGCT 3 cut(s) 64, 94, 703
AoxI GGCC 2 cut(s) 448, 705
ApeKI GCWGC 3 cut(s) 148, 287, 290
ApoI RAATTY 1 cut(s) 710
ArsI GACNNNNNNTTYG 2 cut(s) 419, 451
Asp718I GGTACC 1 cut(s) 27
AspS9I GGNCC 1 cut(s) 157
AsuC2I CCSGG 1 cut(s) 9
AsuHPI GGTGA 1 cut(s) 68
AvaII GGWCC 1 cut(s) 157
BaeGI GKGCMC 1 cut(s) 87
BanI GGYRCC 2 cut(s) 27, 82
BbvI GCAGC 3 cut(s) 135, 299, 302
BccI CCATC 1 cut(s) 511
BceAI ACGGC 1 cut(s) 345
BciVI GTATCC 1 cut(s) 578
BcnI CCSGG 1 cut(s) 9
BfmI CTRYAG 2 cut(s) 149, 726
BfuI GTATCC 1 cut(s) 578
BisI GCNGC 3 cut(s) 149, 288, 291
BlsI GCNGC 3 cut(s) 150, 289, 292
Bme1390I CCNGG 1 cut(s) 9
Bme18I GGWCC 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 157
BmiI GGNNCC 3 cut(s) 6, 29, 84
BmrFI CCNGG 1 cut(s) 9
BmrI ACTGGG 1 cut(s) 47
BmuI ACTGGG 1 cut(s) 47
BplI GAGNNNNNCTC 2 cut(s) 173, 205
BpmI CTGGAG 2 cut(s) 156, 485
BpuMI CCSGG 1 cut(s) 9
BsaJI CCNNGG 1 cut(s) 420
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 3 cut(s) 37, 273, 457
Bse1I ACTGG 3 cut(s) 42, 139, 569
BseDI CCNNGG 1 cut(s) 420
BseLI CCNNNNNNNGG 3 cut(s) 37, 273, 457
BseMII CTCAG 1 cut(s) 214
BseNI ACTGG 3 cut(s) 42, 139, 569
BseSI GKGCMC 1 cut(s) 87
BseXI GCAGC 3 cut(s) 135, 299, 302
BseYI CCCAGC 1 cut(s) 383
BshFI GGCC 2 cut(s) 450, 707
BshNI GGYRCC 2 cut(s) 27, 82
BsiSI CCGG 1 cut(s) 8
BslI CCNNNNNNNGG 3 cut(s) 37, 273, 457
BsnI GGCC 2 cut(s) 450, 707
Bsp1286I GDGCHC 1 cut(s) 87
Bsp1407I TGTACA 1 cut(s) 123
Bsp143I GATC 1 cut(s) 96
BspACI CCGC 1 cut(s) 16
BspANI GGCC 2 cut(s) 450, 707
BspCNI CTCAG 1 cut(s) 213
BspHI TCATGA 1 cut(s) 213
BspLI GGNNCC 3 cut(s) 6, 29, 84
BspMAI CTGCAG 1 cut(s) 153
BspT107I GGYRCC 2 cut(s) 27, 82
BsrGI TGTACA 1 cut(s) 123
BsrI ACTGG 3 cut(s) 42, 139, 569
BssECI CCNNGG 1 cut(s) 420
BssMI GATC 1 cut(s) 96
BssT1I CCWWGG 1 cut(s) 420
Bst4CI ACNGT 1 cut(s) 400
Bst6I CTCTTC 1 cut(s) 726
BstAUI TGTACA 1 cut(s) 123
BstC8I GCNNGC 2 cut(s) 448, 705
BstDEI CTNAG 1 cut(s) 200
BstKTI GATC 1 cut(s) 99
BstMBI GATC 1 cut(s) 96
BstMWI GCNNNNNNNGC 3 cut(s) 91, 108, 329
BstNSI RCATGY 2 cut(s) 50, 670
BstSCI CCNGG 1 cut(s) 7
BstSFI CTRYAG 2 cut(s) 149, 726
BstSLI GKGCMC 1 cut(s) 87
BstV1I GCAGC 3 cut(s) 135, 299, 302
BstXI CCANNNNNNTGG 1 cut(s) 689
BsuI GTATCC 1 cut(s) 578
BsuRI GGCC 2 cut(s) 450, 707
BtsIMutI CAGTG 3 cut(s) 132, 421, 576
Cac8I GCNNGC 2 cut(s) 448, 705
CciI TCATGA 1 cut(s) 213
Cfr13I GGNCC 1 cut(s) 157
Csp6I GTAC 3 cut(s) 28, 124, 239
CviAII CATG 8 cut(s) 32, 47, 127, 214, 298, 324, 667, 683
CviQI GTAC 3 cut(s) 28, 124, 239
DdeI CTNAG 1 cut(s) 200
DpnI GATC 1 cut(s) 98
DpnII GATC 1 cut(s) 96
DraIII CACNNNGTG 1 cut(s) 457
EaeI YGGCCR 1 cut(s) 705
Eam1104I CTCTTC 1 cut(s) 726
EarI CTCTTC 1 cut(s) 726
Eco130I CCWWGG 1 cut(s) 420
Eco47I GGWCC 1 cut(s) 157
Eco57I CTGAAG 1 cut(s) 397
EcoT14I CCWWGG 1 cut(s) 420
ErhI CCWWGG 1 cut(s) 420
FaeI CATG 8 cut(s) 35, 50, 130, 217, 301, 327, 670, 686
FatI CATG 8 cut(s) 31, 46, 126, 213, 297, 323, 666, 682
Fnu4HI GCNGC 3 cut(s) 149, 288, 291
Fsp4HI GCNGC 3 cut(s) 149, 288, 291
GluI GCNGC 3 cut(s) 149, 288, 291
GsaI CCCAGC 1 cut(s) 387
GsuI CTGGAG 2 cut(s) 156, 485
HaeIII GGCC 2 cut(s) 450, 707
HapII CCGG 1 cut(s) 8
Hin1II CATG 8 cut(s) 35, 50, 130, 217, 301, 327, 670, 686
HincII GTYRAC 1 cut(s) 44
HindII GTYRAC 1 cut(s) 44
HindIII AAGCTT 1 cut(s) 62
HinfI GANTC 3 cut(s) 188, 469, 719
HpaII CCGG 1 cut(s) 8
HphI GGTGA 1 cut(s) 68
Hpy166II GTNNAC 2 cut(s) 44, 124
Hpy188III TCNNGA 4 cut(s) 214, 393, 464, 716
Hpy8I GTNNAC 2 cut(s) 44, 124
Hpy99I CGWCG 1 cut(s) 431
HpyAV CCTTC 2 cut(s) 148, 495
HpyCH4III ACNGT 1 cut(s) 400
HpyCH4IV ACGT 1 cut(s) 429
HpyCH4V TGCA 3 cut(s) 151, 323, 446
HpyF10VI GCNNNNNNNGC 3 cut(s) 91, 108, 329
HpyF3I CTNAG 1 cut(s) 200
HpySE526I ACGT 1 cut(s) 429
Hsp92II CATG 8 cut(s) 35, 50, 130, 217, 301, 327, 670, 686
KpnI GGTACC 1 cut(s) 31
Kzo9I GATC 1 cut(s) 96
Lsp1109I GCAGC 3 cut(s) 135, 299, 302
MaeII ACGT 1 cut(s) 429
MaeIII GTNAC 3 cut(s) 130, 203, 308
MalI GATC 1 cut(s) 98
MboI GATC 1 cut(s) 96
MboII GAAGA 2 cut(s) 403, 520
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 1 cut(s) 710
MlyI GAGTC 1 cut(s) 182
MnlI CCTC 4 cut(s) 131, 235, 516, 604
MseI TTAA 3 cut(s) 305, 405, 623
MslI CAYNNNNRTG 3 cut(s) 345, 548, 656
MspA1I CMGCKG 2 cut(s) 94, 703
MspI CCGG 1 cut(s) 8
MspR9I CCNGG 1 cut(s) 9
MwoI GCNNNNNNNGC 3 cut(s) 91, 108, 329
NciI CCSGG 1 cut(s) 9
NdeII GATC 1 cut(s) 96
NlaIII CATG 8 cut(s) 35, 50, 130, 217, 301, 327, 670, 686
NlaIV GGNNCC 3 cut(s) 6, 29, 84
NmuCI GTSAC 1 cut(s) 130
NspI RCATGY 2 cut(s) 50, 670
PagI TCATGA 1 cut(s) 213
PciI ACATGT 1 cut(s) 666
PfeI GAWTC 2 cut(s) 469, 719
PflMI CCANNNNNTGG 2 cut(s) 37, 457
PfoI TCCNGGA 1 cut(s) 7
PkrI GCNGC 3 cut(s) 150, 289, 292
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
PscI ACATGT 1 cut(s) 666
PspFI CCCAGC 1 cut(s) 383
PspN4I GGNNCC 3 cut(s) 6, 29, 84
PspPI GGNCC 1 cut(s) 157
PstI CTGCAG 1 cut(s) 153
PvuII CAGCTG 2 cut(s) 94, 703
RsaI GTAC 3 cut(s) 29, 125, 240
RsaNI GTAC 3 cut(s) 28, 124, 239
RseI CAYNNNNRTG 3 cut(s) 345, 548, 656
SaqAI TTAA 3 cut(s) 305, 405, 623
SatI GCNGC 3 cut(s) 149, 288, 291
Sau3AI GATC 1 cut(s) 96
Sau96I GGNCC 1 cut(s) 157
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 1 cut(s) 9
SduI GDGCHC 1 cut(s) 87
SetI ASST 8 cut(s) 29, 66, 96, 159, 432, 496, 643, 705
SfcI CTRYAG 2 cut(s) 149, 726
SinI GGWCC 1 cut(s) 157
SmiMI CAYNNNNRTG 3 cut(s) 345, 548, 656
Sse9I AATT 1 cut(s) 710
SsiI CCGC 1 cut(s) 16
StyD4I CCNGG 1 cut(s) 7
StyI CCWWGG 1 cut(s) 420
TaaI ACNGT 1 cut(s) 400
TaiI ACGT 1 cut(s) 432
TaqI TCGA 1 cut(s) 506
TasI AATT 1 cut(s) 710
TatI WGTACW 2 cut(s) 123, 238
TfiI GAWTC 2 cut(s) 469, 719
Tru1I TTAA 3 cut(s) 305, 405, 623
Tru9I TTAA 3 cut(s) 305, 405, 623
TscAI CASTG 3 cut(s) 139, 421, 576
TseFI GTSAC 1 cut(s) 130
TseI GCWGC 3 cut(s) 148, 287, 290
Tsp45I GTSAC 1 cut(s) 130
TspDTI ATGAA 2 cut(s) 48, 199
TspRI CASTG 3 cut(s) 139, 421, 576
Van91I CCANNNNNTGG 2 cut(s) 37, 457
VpaK11BI GGWCC 1 cut(s) 157
XapI RAATTY 1 cut(s) 710
XceI RCATGY 2 cut(s) 50, 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.