MD17G1040500.v1.1

Catalytic subunit of the ferredoxin-thioredoxin reductase (FTR), which catalyzes the two-electron reduction of thioredoxins by the electrons provided by reduced ferredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015222
Physical Location & Seq
Reverse (-)
2945852 .. 2948437
2586 bp
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UTR
Exon/CDS
Intron
MD17G1040500.v1.1.491

Sequence Viewer

Length: 441 bp
ATGACTCTGCAACTCCAAGCTCTGTCGTTCGGCGCCAGCATTGCGTCCTTCGTTTCTCCTCTCAACCGCTCCACGCGCCGCCCTGTAATCATAGCCAATGCGGAGCCGTCAGATAAGTCGGTTGAAATCATGAGGAAGTTCTCTGAGCAGTATGCTCGCAAGTCCGGAACATATTTCTGCGTCGATAAGGGAGTTACTTCTGTTGTTATCAAGGGATTGGCAGACCATAAAGATTCATTGGGAGCTCCGCTCTGCCCTTGTCGGCATTATGATGATAAACCTGCCGAGGCAGGCCAAGGCTTTTGGAACTGCCCATGTGTTCCTATGAGAGAGAGGAAGGAATGCCACTGCATGCTCTTTCTCACTCCGGACAATGATTTTGCCGGACCGGAACAGGCAATCTCCCTGGAAGAGATCAAAGAATCAACAGCAAACATGTAA

Protein Analysis

147

Amino Acids

16.15

Weight (kDa)

6.29

Isoelectric Point (pI)

52.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FeThRed_B PF02943 41 - 141 2.1e-42 Ferredoxin thioredoxin reductase catalytic beta chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015222)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04700 AT2G04700
fragaria_vesca FvH4_6g50060
malus_domestica MD09G1039900.v1.1 MD17G1040500.v1.1
prunus_persica Prupe.3G278500_v2.0.a1
pyrus_communis pycom111g03120 pycom17g03630
rosa_chinensis RchiOBHm_Chr2g0170331
rosa_laevigata RLG00000021946
rosa_multiflora Rmu_sc0012445.1_g000004
rosa_roxburghii Rroxscaffold_2G00081430
rosa_rugosa Rorug02G0548200
rosa_samantha Rh2AG619500 Rh2BG630400
rosa_wichuraiana Rw2G051320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 289
AccB1I GGYRCC 1 cut(s) 32
AccBSI CCGCTC 2 cut(s) 69, 250
AccII CGCG 1 cut(s) 76
AccIII TCCGGA 2 cut(s) 164, 367
AciI CCGC 4 cut(s) 67, 79, 101, 248
AcyI GRCGYC 1 cut(s) 33
AflIII ACRYGT 1 cut(s) 435
AgsI TTSAA 1 cut(s) 125
AjnI CCWGG 1 cut(s) 405
AluBI AGCT 2 cut(s) 20, 245
AluI AGCT 2 cut(s) 20, 245
Alw21I GWGCWC 1 cut(s) 247
Aor13HI TCCGGA 2 cut(s) 164, 367
AoxI GGCC 1 cut(s) 292
ArsI GACNNNNNNTTYG 2 cut(s) 362, 394
AspLEI GCGC 2 cut(s) 35, 78
AspS9I GGNCC 1 cut(s) 386
AvaII GGWCC 1 cut(s) 386
BanI GGYRCC 1 cut(s) 32
BanII GRGCYC 1 cut(s) 247
Bbv12I GWGCWC 1 cut(s) 247
BceAI ACGGC 1 cut(s) 91
BcgI CGANNNNNNTGC 2 cut(s) 137, 171
BciT130I CCWGG 1 cut(s) 407
BfoI RGCGCY 1 cut(s) 36
BfuAI ACCTGC 1 cut(s) 289
BisI GCNGC 1 cut(s) 79
BlsI GCNGC 1 cut(s) 80
Bme1390I CCNGG 1 cut(s) 407
Bme18I GGWCC 1 cut(s) 386
BmgT120I GGNCC 1 cut(s) 386
BmiI GGNNCC 2 cut(s) 34, 105
BmrFI CCNGG 1 cut(s) 407
BplI GAGNNNNNCTC 2 cut(s) 234, 266
BsaHI GRCGYC 1 cut(s) 33
BsaJI CCNNGG 3 cut(s) 285, 295, 405
BsaWI WCCGGW 3 cut(s) 164, 367, 388
Bse3DI GCAATG 1 cut(s) 39
BseAI TCCGGA 2 cut(s) 164, 367
BseBI CCWGG 1 cut(s) 407
BseDI CCNNGG 3 cut(s) 285, 295, 405
BseMI GCAATG 1 cut(s) 39
BseMII CTCAG 1 cut(s) 135
BseRI GAGGAG 1 cut(s) 48
Bsh1236I CGCG 1 cut(s) 76
BshFI GGCC 1 cut(s) 294
BshNI GGYRCC 1 cut(s) 32
BsiHKAI GWGCWC 1 cut(s) 247
BsiSI CCGG 4 cut(s) 165, 368, 384, 389
BsmI GAATGC 1 cut(s) 347
BsnI GGCC 1 cut(s) 294
Bsp1286I GDGCHC 1 cut(s) 247
Bsp13I TCCGGA 2 cut(s) 164, 367
Bsp143I GATC 1 cut(s) 414
BspACI CCGC 4 cut(s) 67, 79, 101, 248
BspANI GGCC 1 cut(s) 294
BspCNI CTCAG 1 cut(s) 136
BspEI TCCGGA 2 cut(s) 164, 367
BspFNI CGCG 1 cut(s) 76
BspHI TCATGA 1 cut(s) 129
BspLI GGNNCC 2 cut(s) 34, 105
BspMI ACCTGC 1 cut(s) 289
BspT107I GGYRCC 1 cut(s) 32
BsrBI CCGCTC 2 cut(s) 69, 250
BsrDI GCAATG 1 cut(s) 39
BssECI CCNNGG 3 cut(s) 285, 295, 405
BssMI GATC 1 cut(s) 414
BssNI GRCGYC 1 cut(s) 33
BssT1I CCWWGG 1 cut(s) 295
Bst2UI CCWGG 1 cut(s) 407
Bst6I CTCTTC 1 cut(s) 405
BstACI GRCGYC 1 cut(s) 33
BstC8I GCNNGC 4 cut(s) 37, 157, 292, 353
BstDEI CTNAG 1 cut(s) 144
BstFNI CGCG 1 cut(s) 76
BstH2I RGCGCY 1 cut(s) 36
BstHHI GCGC 2 cut(s) 35, 78
BstKTI GATC 1 cut(s) 417
BstMBI GATC 1 cut(s) 414
BstMWI GCNNNNNNNGC 2 cut(s) 41, 75
BstNI CCWGG 1 cut(s) 407
BstNSI RCATGY 2 cut(s) 355, 439
BstSCI CCNGG 1 cut(s) 405
BstUI CGCG 1 cut(s) 76
BsuRI GGCC 1 cut(s) 294
BtsI GCAGTG 1 cut(s) 346
BtsIMutI CAGTG 1 cut(s) 346
BveI ACCTGC 1 cut(s) 289
Cac8I GCNNGC 4 cut(s) 37, 157, 292, 353
CciI TCATGA 1 cut(s) 129
CfoI GCGC 2 cut(s) 35, 78
Cfr13I GGNCC 1 cut(s) 386
CpoI CGGWCCG 1 cut(s) 386
CseI GACGC 2 cut(s) 33, 169
CspI CGGWCCG 1 cut(s) 386
CviAII CATG 4 cut(s) 130, 315, 352, 436
CviJI RGCY 6 cut(s) 20, 95, 106, 245, 294, 300
CviKI_1 RGCY 6 cut(s) 20, 95, 106, 245, 294, 300
DdeI CTNAG 1 cut(s) 144
DinI GGCGCC 1 cut(s) 34
DpnI GATC 1 cut(s) 416
DpnII GATC 1 cut(s) 414
Eam1104I CTCTTC 1 cut(s) 405
EarI CTCTTC 1 cut(s) 405
Ecl136II GAGCTC 1 cut(s) 245
Eco130I CCWWGG 1 cut(s) 295
Eco24I GRGCYC 1 cut(s) 247
Eco47I GGWCC 1 cut(s) 386
Eco53kI GAGCTC 1 cut(s) 245
EcoICRI GAGCTC 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 405
EcoT14I CCWWGG 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 247
EgeI GGCGCC 1 cut(s) 34
EheI GGCGCC 1 cut(s) 34
ErhI CCWWGG 1 cut(s) 295
FaeI CATG 4 cut(s) 133, 318, 355, 439
FatI CATG 4 cut(s) 129, 314, 351, 435
Fnu4HI GCNGC 1 cut(s) 79
FriOI GRGCYC 1 cut(s) 247
Fsp4HI GCNGC 1 cut(s) 79
GlaI GCGC 2 cut(s) 34, 77
GluI GCNGC 1 cut(s) 79
HaeII RGCGCY 1 cut(s) 36
HaeIII GGCC 1 cut(s) 294
HapII CCGG 4 cut(s) 165, 368, 384, 389
HgaI GACGC 2 cut(s) 33, 169
HhaI GCGC 2 cut(s) 35, 78
Hin1I GRCGYC 1 cut(s) 33
Hin1II CATG 4 cut(s) 133, 318, 355, 439
Hin6I GCGC 2 cut(s) 33, 76
HinP1I GCGC 2 cut(s) 33, 76
HinfI GANTC 3 cut(s) 4, 233, 422
HpaII CCGG 4 cut(s) 165, 368, 384, 389
Hpy188I TCNGA 2 cut(s) 112, 145
Hpy188III TCNNGA 3 cut(s) 130, 165, 368
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 2 cut(s) 58, 331
HpyCH4V TGCA 2 cut(s) 10, 351
HpyF10VI GCNNNNNNNGC 2 cut(s) 41, 75
HpyF3I CTNAG 1 cut(s) 144
Hsp92I GRCGYC 1 cut(s) 33
Hsp92II CATG 4 cut(s) 133, 318, 355, 439
HspAI GCGC 2 cut(s) 33, 76
KasI GGCGCC 1 cut(s) 32
Kpn2I TCCGGA 2 cut(s) 164, 367
Kzo9I GATC 1 cut(s) 414
LmnI GCTCC 4 cut(s) 74, 103, 242, 250
MaeIII GTNAC 1 cut(s) 193
MalI GATC 1 cut(s) 416
MbiI CCGCTC 2 cut(s) 69, 250
MboI GATC 1 cut(s) 414
MboII GAAGA 1 cut(s) 422
MhlI GDGCHC 1 cut(s) 247
Mly113I GGCGCC 1 cut(s) 33
MnlI CCTC 4 cut(s) 69, 126, 280, 327
MroI TCCGGA 2 cut(s) 164, 367
MslI CAYNNNNRTG 1 cut(s) 270
MspI CCGG 4 cut(s) 165, 368, 384, 389
MspR9I CCNGG 1 cut(s) 407
Mva1269I GAATGC 1 cut(s) 347
MvaI CCWGG 1 cut(s) 407
MvnI CGCG 1 cut(s) 76
MwoI GCNNNNNNNGC 2 cut(s) 41, 75
NarI GGCGCC 1 cut(s) 33
NdeII GATC 1 cut(s) 414
NlaIII CATG 4 cut(s) 133, 318, 355, 439
NlaIV GGNNCC 2 cut(s) 34, 105
NmeAIII GCCGAG 1 cut(s) 310
NspI RCATGY 2 cut(s) 355, 439
PaeI GCATGC 1 cut(s) 355
PagI TCATGA 1 cut(s) 129
PciI ACATGT 1 cut(s) 435
PctI GAATGC 1 cut(s) 347
PfeI GAWTC 2 cut(s) 233, 422
PkrI GCNGC 1 cut(s) 80
PluTI GGCGCC 1 cut(s) 36
PscI ACATGT 1 cut(s) 435
Psp124BI GAGCTC 1 cut(s) 247
Psp6I CCWGG 1 cut(s) 405
PspGI CCWGG 1 cut(s) 405
PspN4I GGNNCC 2 cut(s) 34, 105
PspPI GGNCC 1 cut(s) 386
RseI CAYNNNNRTG 1 cut(s) 270
Rsr2I CGGWCCG 1 cut(s) 386
RsrII CGGWCCG 1 cut(s) 386
SacI GAGCTC 1 cut(s) 247
SatI GCNGC 1 cut(s) 79
Sau3AI GATC 1 cut(s) 414
Sau96I GGNCC 1 cut(s) 386
ScrFI CCNGG 1 cut(s) 407
SduI GDGCHC 1 cut(s) 247
SetI ASST 3 cut(s) 22, 247, 283
SfoI GGCGCC 1 cut(s) 34
SinI GGWCC 1 cut(s) 386
SmiMI CAYNNNNRTG 1 cut(s) 270
SphI GCATGC 1 cut(s) 355
SsiI CCGC 4 cut(s) 67, 79, 101, 248
SspDI GGCGCC 1 cut(s) 32
SstI GAGCTC 1 cut(s) 247
StyD4I CCNGG 1 cut(s) 405
StyI CCWWGG 1 cut(s) 295
TaqI TCGA 1 cut(s) 183
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 2 cut(s) 233, 422
TscAI CASTG 1 cut(s) 353
TspDTI ATGAA 1 cut(s) 225
TspRI CASTG 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 386
XceI RCATGY 2 cut(s) 355, 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.