Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0015222

Physical Location & Seq

Genomic Coordinates Reverse (-)

67599243 .. 67602305

Gene Structure 3063 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0548200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3063 bp

ATGCTTCACTGCCAGAGCTACAATTCCAGAGAATTAGGGCAAGAAAGTCTCCACAGCTCAATGCAAGGCCACACCTTTTCACCAAGTAAGCTTCAAAACCCCCAATCTAATTTCACTACTAGGCCTCTTCTAGGGTTTCGTTTCACTCCCCACAACCATAGCTTAGCTAAAACCCAACTATGCCCTGTTCCAATTTCTCAAAAGGGTAGCTGTATTGTGGGTTCACGACCTCAGAAGCAGGGTTCAAGAGGGTCTAGGGTTTATGTAGGGTTTAAGCTTCAGTGTCATTCGAAAGCATTGGTTTTACCCACGAAGGTTTCTTTAGTTAATGGTAAGAAGAAGAGGTATGGAGGCGTGTTGCCTTCGATTTTGAGGTCTTTGGAGTCTGAAAACGATGTTGAAAAGGCACTCAAGTCGTTTGGGGAGAGTCTGAGTGCGAAAGAACAGACTGTGATTCTCAAAGAACAGAGGAGCTGGGAGAGAGTTCTTCGTGTTTTCGAGTGGTTCAAGTCACAGAAAGAGTATGTGCCCAATGTGATTCACTACAATGTGGTGCTTCGGGTGCTTGGTAGAGCTCAGAGATGGGATGAGCTGAGGCTTTGTTGGGTTGAAATGGCGAAAAAGGGTGTCCTGCCGACTAACAATACGTATTCCATGCTTGTTGATGTGTATGGGAAAGCGGGACTTGTGAAGGAGGCCCTGTTGTGGATTAAGCACATGAAGCTAAGGGGACTTTTCCCGGATGAGGTTACAATGAATACGGTTGTTCGGGCATTGAAGAATGCAGAAGAGTTTGATAGGGCGGATAAGTTTTATAAGGACTGGTGTACTGGGCGGATTGAGCTCGATGACCTTGAGTTGGATTCTATGGCTGATTCTGTGATTGGTTCTGTTTCGGAACCTATTAGTTTCAAGCATTTCTTGTCCACTGAGCTATTCAGGACTGGTGGGAGAGTTTCCACTTCAAAAATTATGACCTCAATGGATACAGAGAACTCTATTCAGAAACCACGACTTACATCTACGTATAATAGTTTGATTGATTTGTATGGAAAGGCAGGGCGTCTGAATGATGCGGCTAATGTGTTTGGAGATATGATGAAGTCTGGAGTGGCAATGGATGTTATTACTTTTAATACTATGATCTTTACTTGTGGAAGTCATGGGCATTTGTTGGAGGCGGAAGCTTTGCTTAGTAAGATGGAAGAAAGGGGTATATCTCCTGATACAAGAACTTATAACATTTTTCTATCTCTGTATGCTGATGTGGGGAACATTGATGCTGCTCTGAACTGCTACAGGAAGATCAGAGAGGTAGGTCTTTACCCGGATATTGTATCTCACCGGACTATTCTTCATGTATTATGTGAGAGAAACATGGTCAGAGATGTGGAGATTGTGATTGAGGATATGGAGAAATCTGGTGTCTCTATTAATGAGCATTCTCTTCCTGGTATCATCAAGATGTATATTAATGAAGGACGGCTTGATCAGGCAAACTTACTTTATGAGAAATGTCAGTTAAACCGTGGGATTTCATCAAAGACGCATGCAGCAATTATAGATGCATATGCTGAAAAGGGACTTTGGACTGAAGCCGAGGTCGTATTCTATAGGAAGGGAGATTTGGTTGGACAGACGAAGGATATTGTGGAATACAATGTTATGATCAAAGCGTATGGTAAGGCAAGGCTTTATGATAAAGCTTTCTGTCTCTTCAGGGGCATGAAAAAGCATGGGACTTGGCCCGATGAGTGCACTTACAACTCTCTCATCCAAATGTTCTCCGGAGGTGATTTAGTGGACCAAGCAAGGGATCTTCTAACAGAAATGCAGGAAACTGGTCTGAAGCCACAATCTCTAACCTTTTCTGCTCTTATTGCCTGCTATGCTCGCCTTGGCCAGCTTTCAGATGCAGTTGATGTGTACCAAGACTTTGTAAAGTCAGGAACAAAGCCAAATGAGTTTGTGTACGGTTCTTTAATCAATGGATTCGCAGAAACTGGCAGAGTTGAAGAAGCACTGAAGTATTTTCACCACATGGAAAAATCAGGGATAACTGCAAATCAAATAGTTCTGACATCTCTGATAAAGGCGTATAGTAAGGCGGGAAGCCGTGATGGAGCAGAAGTGTTGTATGAGAGGTTGAAAGGTTTTGATGGTGGCCCAGATGTTGTTGCATCTAACAGTATGATAAATCTGTATGCAGATCTTGGTTTGGTATCTGAAGCCAAATTGATTTTTGAAAATTTGAGAGCGAAAGGTTGGGCTGATGAGATAGCGTTTGCAACCATGATGTATCTGTACAAGAGCATGGGCATGCTTGATGAGGCCATTGATGTAGCAGAGGAAATGAAAGAATCGGGTTTAATAAGGGACTGCGCTTCATTTAACAAGGTGATGTCATGCTATGCCATTAACGGGCAGCTACGTGAATGCGGTGAATTATTGCACGAGATGGTAACTCGGAAACTGTTGCTTGACAGTGGAACTTGTAATGTATTATTCACAGTATTGAGAAAAGGAGGTATTCCCATTGAAGCTGTAACCCAGCTAGAGTCATCTTACCAGGAAGGAAAACCTTACTCTAGACAAGCTATCATCACCTCTGTATTGTCTCTGGTTGGTATGCATGGTTTGGCACTCGAGTCCTGTGAAACATTCACAAAAGCTGACATAAATCTTGATACTTCTCTCTACAATGTTGCGATATATGCTTATGGTGCAGCTGGGGAGATTGACAAGGCTCTGAACATATTTATGAGAATGCAGGATGAAGGTGTGGAACCTGACCTTGTAACGCATATTTTCCTAGTAGGTTGTTATGGAAAAGCAGGTATGGTTGAAGGAGTGAAGCGAATTTACAGCCAGCTGAAATATGAAGAGATAGAGCCTAATGCGTCCTTGTTCAGAGCTGTTATAGATGCCTATACAGATGCCAATAGGCATGACCTTGCCAAATTGGTTAAGCAAGACAGGAAATATGCATTTGACTCGGAACATCATGTAGATTCCGAAACCAAAGATGAGTTTGATGGCACTACTTCAGAGTTTGAGGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0008150 GO:0008152 GO:0009055 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0009987 GO:0016491 GO:0016730 GO:0022900 GO:0030385 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048037 GO:0051536 GO:0051539 GO:0051540 GO:0055114

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FeThRed_B

Protein Analysis

1020

Amino Acids

114.48

Weight (kDa)

6.12

Isoelectric Point (pI)

32.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	177 - 224	1.1e-07	PPR repeat family
PPR_long	PF17177	340 - 506	4.8e-09	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	341 - 385	9.9e-12	PPR repeat family
PPR	PF01535	341 - 371	4.7e-07	PPR repeat
PPR_1	PF12854	341 - 366	9.5e-06	PPR repeat
PPR_1	PF12854	370 - 402	8.3e-06	PPR repeat
PPR_2	PF13041	374 - 420	7.7e-12	PPR repeat family
PPR_3	PF13812	397 - 455	9.4e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	409 - 456	5e-07	PPR repeat family
PPR_2	PF13041	548 - 595	1.6e-12	PPR repeat family
PPR_3	PF13812	549 - 596	6.8e-07	Pentatricopeptide repeat domain
TPR_24	PF23276	551 - 646	5.5e-09	Fungal tetratrico peptide repeats
PPR	PF01535	553 - 580	3.1e-06	PPR repeat
PPR_long	PF17177	566 - 679	1.5e-10	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	583 - 632	5e-09	PPR repeat family
PPR_2	PF13041	618 - 666	3.8e-09	PPR repeat family
PPR_1	PF12854	650 - 682	3.4e-06	PPR repeat
PPR_2	PF13041	654 - 702	2.3e-08	PPR repeat family
PPR	PF01535	657 - 686	1.1e-06	PPR repeat
TPR_24	PF23276	867 - 982	3.1e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	890 - 944	5e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	896 - 939	4.8e-07	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04700 AT2G04700
fragaria_vesca	FvH4_6g50060
malus_domestica	MD09G1039900.v1.1 MD17G1040500.v1.1
prunus_persica	Prupe.3G278500_v2.0.a1
pyrus_communis	pycom111g03120 pycom17g03630
rosa_chinensis	RchiOBHm_Chr2g0170331
rosa_laevigata	RLG00000021946
rosa_multiflora	Rmu_sc0012445.1_g000004
rosa_roxburghii	Rroxscaffold_2G00081430
rosa_rugosa	Rorug02G0548200
rosa_samantha	Rh2AG619500 Rh2BG630400
rosa_wichuraiana	Rw2G051320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	816, 1239
Acc36I	ACCTGC	1 cut(s)	2825
AccIII	TCCGGA	1 cut(s)	1787
AciI	CCGC	7 cut(s)	680, 803, 835, 1076, 1181, 2108, 2439
AclWI	GGATC	1 cut(s)	1824
AcoI	YGGCCR	1 cut(s)	1900
AcsI	RAATTY	2 cut(s)	2248, 2859
AcuI	CTGAAG	7 cut(s)	263, 1614, 1702, 1868, 2045, 2247, 3030
AcyI	GRCGYC	1 cut(s)	1063
AfaI	GTAC	4 cut(s)	829, 1928, 1973, 2306
AfiI	CCNNNNNNNGG	6 cut(s)	131, 705, 745, 859, 1813, 2421
AjnI	CCWGG	2 cut(s)	1450, 2568
AjuI	GAANNNNNNNTTGG	2 cut(s)	1610, 1642
Alw21I	GWGCWC	3 cut(s)	577, 846, 1760
Alw26I	GTCTC	4 cut(s)	53, 1432, 1718, 2622
Alw44I	GTGCAC	1 cut(s)	1756
AlwI	GGATC	1 cut(s)	1824
AlwNI	CAGNNNCTG	1 cut(s)	2003
Ama87I	CYCGRG	1 cut(s)	2645
Aor13HI	TCCGGA	1 cut(s)	1787
AoxI	GGCC	7 cut(s)	67, 122, 696, 1745, 1900, 2164, 2331
ApaLI	GTGCAC	1 cut(s)	1756
ApeKI	GCWGC	4 cut(s)	1283, 1553, 2425, 2726
ApoI	RAATTY	2 cut(s)	2248, 2859
AseI	ATTAAT	2 cut(s)	1434, 1473
AspLEI	GCGC	1 cut(s)	2384
AspS9I	GGNCC	4 cut(s)	697, 1746, 1804, 2165
AsuC2I	CCSGG	2 cut(s)	740, 1328
AsuHPI	GGTGA	7 cut(s)	72, 1334, 1805, 2027, 2410, 2453, 2596
AsuII	TTCGAA	1 cut(s)	290
AvaI	CYCGRG	1 cut(s)	2645
AvaII	GGWCC	1 cut(s)	1804
BaeGI	GKGCMC	2 cut(s)	531, 1760
BalI	TGGCCA	1 cut(s)	1902
BanII	GRGCYC	2 cut(s)	577, 846
BarI	GAAGNNNNNNTAC	2 cut(s)	2846, 2878
BauI	CACGAG	1 cut(s)	2453
Bbv12I	GWGCWC	3 cut(s)	577, 846, 1760
BbvCI	CCTCAGC	1 cut(s)	593
BbvI	GCAGC	4 cut(s)	1270, 1565, 2437, 2738
BccI	CCATC	6 cut(s)	576, 1195, 2114, 2153, 2452, 3029
BceAI	ACGGC	2 cut(s)	1499, 2100
BcgI	CGANNNNNNTGC	4 cut(s)	396, 430, 2976, 3010
BciT130I	CCWGG	2 cut(s)	1452, 2570
BciVI	GTATCC	1 cut(s)	979
BclI	TGATCA	2 cut(s)	1489, 1668
BcnI	CCSGG	2 cut(s)	740, 1328
BcoDI	GTCTC	4 cut(s)	53, 1432, 1718, 2622
BfaI	CTAG	6 cut(s)	120, 131, 255, 2555, 2589, 2813
BfmI	CTRYAG	2 cut(s)	1297, 1612
BfuAI	ACCTGC	1 cut(s)	2825
BfuI	GTATCC	1 cut(s)	979
BglII	AGATCT	1 cut(s)	2209
BisI	GCNGC	5 cut(s)	1077, 1284, 1554, 2426, 2727
BlpI	GCTNAGC	1 cut(s)	163
BlsI	GCNGC	5 cut(s)	1078, 1285, 1555, 2427, 2728
Bme1390I	CCNGG	4 cut(s)	740, 1328, 1452, 2570
Bme18I	GGWCC	1 cut(s)	1804
BmeT110I	CYCGRG	1 cut(s)	2645
BmgT120I	GGNCC	4 cut(s)	697, 1746, 1804, 2165
BmiI	GGNNCC	2 cut(s)	900, 2787
BmrFI	CCNGG	4 cut(s)	740, 1328, 1452, 2570
BmrI	ACTGGG	1 cut(s)	840
BmsI	GCATC	7 cut(s)	1063, 1270, 1555, 1903, 2189, 2914, 2926
BmuI	ACTGGG	1 cut(s)	840
BpmI	CTGGAG	1 cut(s)	1128
Bpu10I	CCTNAGC	2 cut(s)	593, 725
Bpu1102I	GCTNAGC	1 cut(s)	163
Bpu14I	TTCGAA	1 cut(s)	290
BpuEI	CTTGAG	2 cut(s)	395, 875
BpuMI	CCSGG	2 cut(s)	740, 1328
BsaAI	YACGTR	3 cut(s)	648, 1026, 2432
BsaHI	GRCGYC	1 cut(s)	1063
BsaJI	CCNNGG	3 cut(s)	1528, 1599, 1897
BsaWI	WCCGGW	2 cut(s)	1344, 1787
BsaXI	ACNNNNNCTCC	6 cut(s)	686, 716, 1614, 1644, 2115, 2145
Bsc4I	CCNNNNNNNGG	6 cut(s)	131, 705, 745, 859, 1813, 2421
Bse1I	ACTGG	5 cut(s)	827, 835, 949, 1846, 2008
Bse3DI	GCAATG	1 cut(s)	1122
BseAI	TCCGGA	1 cut(s)	1787
BseBI	CCWGG	2 cut(s)	1452, 2570
BseDI	CCNNGG	3 cut(s)	1528, 1599, 1897
BseGI	GGATG	5 cut(s)	592, 748, 1126, 1773, 2779
BseLI	CCNNNNNNNGG	6 cut(s)	131, 705, 745, 859, 1813, 2421
BseMI	GCAATG	1 cut(s)	1122
BseMII	CTCAG	5 cut(s)	245, 422, 584, 590, 921
BseNI	ACTGG	5 cut(s)	827, 835, 949, 1846, 2008
BseRI	GAGGAG	1 cut(s)	484
BseSI	GKGCMC	2 cut(s)	531, 1760
BseXI	GCAGC	4 cut(s)	1270, 1565, 2437, 2738
BseYI	CCCAGC	3 cut(s)	474, 2550, 2729
BsgI	GTGCAG	1 cut(s)	2745
BshFI	GGCC	7 cut(s)	69, 124, 698, 1747, 1902, 2166, 2333
BsiHKAI	GWGCWC	3 cut(s)	577, 846, 1760
BsiHKCI	CYCGRG	1 cut(s)	2645
BsiSI	CCGG	4 cut(s)	740, 1328, 1345, 1788
BslFI	GGGAC	5 cut(s)	696, 744, 1596, 1753, 2390
BslI	CCNNNNNNNGG	6 cut(s)	131, 705, 745, 859, 1813, 2421
BsmAI	GTCTC	4 cut(s)	53, 1432, 1718, 2622
BsmFI	GGGAC	5 cut(s)	696, 744, 1596, 1753, 2390
BsmI	GAATGC	4 cut(s)	787, 1441, 2441, 2772
BsnI	GGCC	7 cut(s)	69, 124, 698, 1747, 1902, 2166, 2333
BsoBI	CYCGRG	1 cut(s)	2645
Bsp119I	TTCGAA	1 cut(s)	290
Bsp1286I	GDGCHC	4 cut(s)	531, 577, 846, 1760
Bsp13I	TCCGGA	1 cut(s)	1787
Bsp1407I	TGTACA	1 cut(s)	2304
Bsp143I	GATC	6 cut(s)	1143, 1305, 1489, 1668, 1816, 2209
Bsp1720I	GCTNAGC	1 cut(s)	163
BspACI	CCGC	7 cut(s)	680, 803, 835, 1076, 1181, 2108, 2439
BspANI	GGCC	7 cut(s)	69, 124, 698, 1747, 1902, 2166, 2333
BspCNI	CTCAG	5 cut(s)	244, 423, 585, 589, 922
BspEI	TCCGGA	1 cut(s)	1787
BspLI	GGNNCC	2 cut(s)	900, 2787
BspMI	ACCTGC	1 cut(s)	2825
BspPI	GGATC	1 cut(s)	1824
BspT104I	TTCGAA	1 cut(s)	290
BsrDI	GCAATG	1 cut(s)	1122
BsrGI	TGTACA	1 cut(s)	2304
BsrI	ACTGG	5 cut(s)	827, 835, 949, 1846, 2008
BssECI	CCNNGG	3 cut(s)	1528, 1599, 1897
BssMI	GATC	6 cut(s)	1143, 1305, 1489, 1668, 1816, 2209
BssNI	GRCGYC	1 cut(s)	1063
BssSI	CACGAG	1 cut(s)	2453
BssT1I	CCWWGG	1 cut(s)	1897
Bst2BI	CACGAG	1 cut(s)	2453
Bst2UI	CCWGG	2 cut(s)	1452, 2570
Bst4CI	ACNGT	8 cut(s)	451, 763, 1529, 1976, 2189, 2475, 2486, 2512
Bst6I	CTCTTC	6 cut(s)	132, 335, 783, 1452, 1721, 2877
BstACI	GRCGYC	1 cut(s)	1063
BstAUI	TGTACA	1 cut(s)	2304
BstBAI	YACGTR	3 cut(s)	648, 1026, 2432
BstBI	TTCGAA	1 cut(s)	290
BstC8I	GCNNGC	6 cut(s)	1551, 1885, 1894, 1904, 2321, 2870
BstDEI	CTNAG	8 cut(s)	163, 231, 431, 576, 593, 725, 930, 1193
BstDSI	CCRYGG	1 cut(s)	1528
BstENI	CCTNNNNNAGG	1 cut(s)	129
BstF5I	GGATG	5 cut(s)	592, 748, 1126, 1773, 2779
BstHHI	GCGC	1 cut(s)	2384
BstKTI	GATC	6 cut(s)	1146, 1308, 1492, 1671, 1819, 2212
BstMAI	GTCTC	4 cut(s)	53, 1432, 1718, 2622
BstMBI	GATC	6 cut(s)	1143, 1305, 1489, 1668, 1816, 2209
BstMWI	GCNNNNNNNGC	8 cut(s)	562, 721, 841, 1880, 1889, 1893, 2714, 2723
BstNI	CCWGG	2 cut(s)	1452, 2570
BstNSI	RCATGY	2 cut(s)	1553, 2323
BstSCI	CCNGG	4 cut(s)	738, 1326, 1450, 2568
BstSFI	CTRYAG	2 cut(s)	1297, 1612
BstSLI	GKGCMC	2 cut(s)	531, 1760
BstSNI	TACGTA	2 cut(s)	648, 1026
BstV1I	GCAGC	4 cut(s)	1270, 1565, 2437, 2738
BstX2I	RGATCY	2 cut(s)	1816, 2209
BstYI	RGATCY	2 cut(s)	1816, 2209
BsuI	GTATCC	1 cut(s)	979
BsuRI	GGCC	7 cut(s)	69, 124, 698, 1747, 1902, 2166, 2333
BtgI	CCRYGG	1 cut(s)	1528
BtsCI	GGATG	5 cut(s)	592, 748, 1126, 1773, 2779
BtsI	GCAGTG	1 cut(s)	7
BtsIMutI	CAGTG	5 cut(s)	7, 287, 927, 2021, 2491
BveI	ACCTGC	1 cut(s)	2825
Cac8I	GCNNGC	6 cut(s)	1551, 1885, 1894, 1904, 2321, 2870
CaiI	CAGNNNCTG	1 cut(s)	2003
CfoI	GCGC	1 cut(s)	2384
Cfr13I	GGNCC	4 cut(s)	697, 1746, 1804, 2165
CseI	GACGC	3 cut(s)	1052, 1555, 2889
Csp6I	GTAC	4 cut(s)	828, 1927, 1972, 2305
CviQI	GTAC	4 cut(s)	828, 1927, 1972, 2305
DdeI	CTNAG	8 cut(s)	163, 231, 431, 576, 593, 725, 930, 1193
DpnI	GATC	6 cut(s)	1145, 1307, 1491, 1670, 1818, 2211
DpnII	GATC	6 cut(s)	1143, 1305, 1489, 1668, 1816, 2209
EaeI	YGGCCR	1 cut(s)	1900
Eam1104I	CTCTTC	6 cut(s)	132, 335, 783, 1452, 1721, 2877
EarI	CTCTTC	6 cut(s)	132, 335, 783, 1452, 1721, 2877
EciI	GGCGGA	3 cut(s)	818, 850, 1196
Ecl136II	GAGCTC	2 cut(s)	575, 844
Eco105I	TACGTA	2 cut(s)	648, 1026
Eco130I	CCWWGG	1 cut(s)	1897
Eco147I	AGGCCT	1 cut(s)	124
Eco24I	GRGCYC	2 cut(s)	577, 846
Eco47I	GGWCC	1 cut(s)	1804
Eco53kI	GAGCTC	2 cut(s)	575, 844
Eco57I	CTGAAG	7 cut(s)	263, 1614, 1702, 1868, 2045, 2247, 3030
Eco88I	CYCGRG	1 cut(s)	2645
EcoICRI	GAGCTC	2 cut(s)	575, 844
EcoNI	CCTNNNNNAGG	1 cut(s)	129
EcoO109I	RGGNCCY	1 cut(s)	697
EcoRII	CCWGG	2 cut(s)	1450, 2568
EcoT14I	CCWWGG	1 cut(s)	1897
EcoT22I	ATGCAT	3 cut(s)	1570, 2634, 2989
EcoT38I	GRGCYC	2 cut(s)	577, 846
ErhI	CCWWGG	1 cut(s)	1897
FalI	AAGNNNNNCTT	8 cut(s)	669, 701, 905, 937, 1176, 1208, 1470, 1502
FaqI	GGGAC	5 cut(s)	696, 744, 1596, 1753, 2390
FauI	CCCGC	2 cut(s)	673, 2101
FauNDI	CATATG	1 cut(s)	1570
FbaI	TGATCA	2 cut(s)	1489, 1668
Fnu4HI	GCNGC	5 cut(s)	1077, 1284, 1554, 2426, 2727
FokI	GGATG	5 cut(s)	599, 755, 1133, 1760, 2786
FriOI	GRGCYC	2 cut(s)	577, 846
Fsp4HI	GCNGC	5 cut(s)	1077, 1284, 1554, 2426, 2727
FspBI	CTAG	6 cut(s)	120, 131, 255, 2555, 2589, 2813
GlaI	GCGC	1 cut(s)	2383
GluI	GCNGC	5 cut(s)	1077, 1284, 1554, 2426, 2727
GsaI	CCCAGC	3 cut(s)	478, 2554, 2733
GsuI	CTGGAG	1 cut(s)	1128
HaeIII	GGCC	7 cut(s)	69, 124, 698, 1747, 1902, 2166, 2333
HapII	CCGG	4 cut(s)	740, 1328, 1345, 1788
HgaI	GACGC	3 cut(s)	1052, 1555, 2889
HhaI	GCGC	1 cut(s)	2384
Hin1I	GRCGYC	1 cut(s)	1063
Hin6I	GCGC	1 cut(s)	2382
HinP1I	GCGC	1 cut(s)	2382
HindIII	AAGCTT	4 cut(s)	89, 275, 1185, 1704
HpaII	CCGG	4 cut(s)	740, 1328, 1345, 1788
HphI	GGTGA	7 cut(s)	72, 1334, 1805, 2027, 2410, 2453, 2596
Hpy166II	GTNNAC	7 cut(s)	224, 828, 927, 1758, 1804, 1927, 1972
Hpy8I	GTNNAC	7 cut(s)	224, 828, 927, 1758, 1804, 1927, 1972
HpyAV	CCTTC	9 cut(s)	307, 372, 685, 1472, 1612, 1636, 2567, 2771, 2840
HpyCH4III	ACNGT	8 cut(s)	451, 763, 1529, 1976, 2189, 2475, 2486, 2512
HpyCH4IV	ACGT	3 cut(s)	647, 1025, 2431
HpyF10VI	GCNNNNNNNGC	8 cut(s)	562, 721, 841, 1880, 1889, 1893, 2714, 2723
HpyF3I	CTNAG	8 cut(s)	163, 231, 431, 576, 593, 725, 930, 1193
HpySE526I	ACGT	3 cut(s)	647, 1025, 2431
Hsp92I	GRCGYC	1 cut(s)	1063
HspAI	GCGC	1 cut(s)	2382
Kpn2I	TCCGGA	1 cut(s)	1787
Ksp22I	TGATCA	2 cut(s)	1489, 1668
Kzo9I	GATC	6 cut(s)	1143, 1305, 1489, 1668, 1816, 2209
LmnI	GCTCC	2 cut(s)	471, 2123
Lsp1109I	GCAGC	4 cut(s)	1270, 1565, 2437, 2738
LweI	GCATC	7 cut(s)	1063, 1270, 1555, 1903, 2189, 2914, 2926
MaeI	CTAG	6 cut(s)	120, 131, 255, 2555, 2589, 2813
MaeII	ACGT	3 cut(s)	647, 1025, 2431
MaeIII	GTNAC	5 cut(s)	510, 748, 2461, 2545, 2797
MalI	GATC	6 cut(s)	1145, 1307, 1491, 1670, 1818, 2211
MboI	GATC	6 cut(s)	1143, 1305, 1489, 1668, 1816, 2209
MflI	RGATCY	2 cut(s)	1816, 2209
MhlI	GDGCHC	4 cut(s)	531, 577, 846, 1760
MlsI	TGGCCA	1 cut(s)	1902
MluNI	TGGCCA	1 cut(s)	1902
MlyI	GAGTC	5 cut(s)	392, 436, 2567, 2657, 2987
MmeI	TCCRAC	3 cut(s)	840, 1155, 1612
Mox20I	TGGCCA	1 cut(s)	1902
Mph1103I	ATGCAT	3 cut(s)	1570, 2634, 2989
MroI	TCCGGA	1 cut(s)	1787
MscI	TGGCCA	1 cut(s)	1902
MslI	CAYNNNNRTG	5 cut(s)	546, 1463, 1778, 2318, 2759
Msp20I	TGGCCA	1 cut(s)	1902
MspA1I	CMGCKG	2 cut(s)	2729, 2872
MspI	CCGG	4 cut(s)	740, 1328, 1345, 1788
MspR9I	CCNGG	4 cut(s)	740, 1328, 1452, 2570
Mva1269I	GAATGC	4 cut(s)	787, 1441, 2441, 2772
MvaI	CCWGG	2 cut(s)	1452, 2570
MwoI	GCNNNNNNNGC	8 cut(s)	562, 721, 841, 1880, 1889, 1893, 2714, 2723
NciI	CCSGG	2 cut(s)	740, 1328
NdeI	CATATG	1 cut(s)	1570
NdeII	GATC	6 cut(s)	1143, 1305, 1489, 1668, 1816, 2209
NlaIV	GGNNCC	2 cut(s)	900, 2787
NmeAIII	GCCGAG	1 cut(s)	1624
NmuCI	GTSAC	1 cut(s)	510
NsiI	ATGCAT	3 cut(s)	1570, 2634, 2989
NspI	RCATGY	2 cut(s)	1553, 2323
NspV	TTCGAA	1 cut(s)	290
PaeI	GCATGC	2 cut(s)	1553, 2323
PaeR7I	CTCGAG	1 cut(s)	2645
PceI	AGGCCT	1 cut(s)	124
PctI	GAATGC	4 cut(s)	787, 1441, 2441, 2772
PfeI	GAWTC	7 cut(s)	454, 538, 863, 875, 1992, 2360, 3011
PfoI	TCCNGGA	1 cut(s)	738
PkrI	GCNGC	5 cut(s)	1078, 1285, 1555, 2427, 2728
PleI	GAGTC	5 cut(s)	391, 435, 2566, 2656, 2987
PpsI	GAGTC	5 cut(s)	391, 435, 2566, 2656, 2987
Ppu21I	YACGTR	3 cut(s)	648, 1026, 2432
PshBI	ATTAAT	2 cut(s)	1434, 1473
PsiI	TTATAA	2 cut(s)	816, 1239
Psp124BI	GAGCTC	2 cut(s)	577, 846
Psp6I	CCWGG	2 cut(s)	1450, 2568
PspFI	CCCAGC	3 cut(s)	474, 2550, 2729
PspGI	CCWGG	2 cut(s)	1450, 2568
PspN4I	GGNNCC	2 cut(s)	900, 2787
PspPI	GGNCC	4 cut(s)	697, 1746, 1804, 2165
PspXI	VCTCGAGB	1 cut(s)	2645
PsrI	GAACNNNNNNTAC	2 cut(s)	205, 237
PstNI	CAGNNNCTG	1 cut(s)	2003
PsuI	RGATCY	2 cut(s)	1816, 2209
PvuII	CAGCTG	2 cut(s)	2729, 2872
RsaI	GTAC	4 cut(s)	829, 1928, 1973, 2306
RsaNI	GTAC	4 cut(s)	828, 1927, 1972, 2305
RseI	CAYNNNNRTG	5 cut(s)	546, 1463, 1778, 2318, 2759
SacI	GAGCTC	2 cut(s)	577, 846
SatI	GCNGC	5 cut(s)	1077, 1284, 1554, 2426, 2727
Sau3AI	GATC	6 cut(s)	1143, 1305, 1489, 1668, 1816, 2209
Sau96I	GGNCC	4 cut(s)	697, 1746, 1804, 2165
SchI	GAGTC	5 cut(s)	392, 436, 2567, 2657, 2987
ScrFI	CCNGG	4 cut(s)	740, 1328, 1452, 2570
SduI	GDGCHC	4 cut(s)	531, 577, 846, 1760
SfaNI	GCATC	7 cut(s)	1063, 1270, 1555, 1903, 2189, 2914, 2926
SfcI	CTRYAG	2 cut(s)	1297, 1612
Sfr274I	CTCGAG	1 cut(s)	2645
SfuI	TTCGAA	1 cut(s)	290
SinI	GGWCC	1 cut(s)	1804
SlaI	CTCGAG	1 cut(s)	2645
SmiMI	CAYNNNNRTG	5 cut(s)	546, 1463, 1778, 2318, 2759
SmlI	CTYRAG	3 cut(s)	410, 854, 2645
SmoI	CTYRAG	3 cut(s)	410, 854, 2645
SnaBI	TACGTA	2 cut(s)	648, 1026
SphI	GCATGC	2 cut(s)	1553, 2323
SseBI	AGGCCT	1 cut(s)	124
SsiI	CCGC	7 cut(s)	680, 803, 835, 1076, 1181, 2108, 2439
SspMI	CTAG	6 cut(s)	120, 131, 255, 2555, 2589, 2813
SstI	GAGCTC	2 cut(s)	577, 846
StuI	AGGCCT	1 cut(s)	124
StyD4I	CCNGG	4 cut(s)	738, 1326, 1450, 2568
StyI	CCWWGG	1 cut(s)	1897
TaaI	ACNGT	8 cut(s)	451, 763, 1529, 1976, 2189, 2475, 2486, 2512
TaiI	ACGT	3 cut(s)	650, 1028, 2434
TaqI	TCGA	5 cut(s)	290, 365, 498, 846, 2646
TatI	WGTACW	2 cut(s)	827, 2304
TauI	GCSGC	1 cut(s)	1079
TfiI	GAWTC	7 cut(s)	454, 538, 863, 875, 1992, 2360, 3011
TscAI	CASTG	5 cut(s)	14, 287, 934, 2028, 2491
TseFI	GTSAC	1 cut(s)	510
TseI	GCWGC	4 cut(s)	1283, 1553, 2425, 2726
Tsp45I	GTSAC	1 cut(s)	510
TspRI	CASTG	5 cut(s)	14, 287, 934, 2028, 2491
VneI	GTGCAC	1 cut(s)	1756
VpaK11BI	GGWCC	1 cut(s)	1804
VspI	ATTAAT	2 cut(s)	1434, 1473
XagI	CCTNNNNNAGG	1 cut(s)	129
XapI	RAATTY	2 cut(s)	2248, 2859
XbaI	TCTAGA	1 cut(s)	2588
XceI	RCATGY	2 cut(s)	1553, 2323
XhoI	CTCGAG	1 cut(s)	2645
XspI	CTAG	6 cut(s)	120, 131, 255, 2555, 2589, 2813
Zsp2I	ATGCAT	3 cut(s)	1570, 2634, 2989

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.