Rw2G051320

Catalytic subunit of the ferredoxin-thioredoxin reductase (FTR), which catalyzes the two-electron reduction of thioredoxins by the electrons provided by reduced ferredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0015222
Physical Location & Seq
Forward (+)
79054223 .. 79057094
2872 bp
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UTR
Exon/CDS
Intron
Rw2G051320.1

Sequence Viewer

Length: 441 bp
ATGACTCTCCAGCTCCAAGCTCCGTCGTTCGGCGCCACCATTTCCTCGCTCCCTTCTCCTCTCACCCGCTCCACCCGCCGCTTCGTAATTCGAGCTCAAGTTGAACCAACTGAGAAGTCTGTTGAGATTATGAGGAAGTTTTCGGAGCAGTATGCTCGCAAGTCCGGGACATATTTCTGTGTTGATAAGGGTGTCACTTCTGTTGTTATCAAGGGATTGGCAGACCACAAAGATTCATTGGGTGCTCCACTTTGCCCTTGTCGGCATTACGATGACAAACCGGCAGAGGCTGGCCAAGGCTTTTGGAACTGCCCCTGTGTTCCCATGAGGGAAAGGAAGGAATGCCACTGCATGCTCTTTCTCACCCCTGAAAATGATTTTGCTGGACAAGATCAGACCATCACCTTGGAGGAGATCCGAGAATCGACCGCAAACATCTAA

Protein Analysis

146

Amino Acids

16.36

Weight (kDa)

6.81

Isoelectric Point (pI)

60.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FeThRed_B PF02943 41 - 140 2.8e-42 Ferredoxin thioredoxin reductase catalytic beta chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015222)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04700 AT2G04700
fragaria_vesca FvH4_6g50060
malus_domestica MD09G1039900.v1.1 MD17G1040500.v1.1
prunus_persica Prupe.3G278500_v2.0.a1
pyrus_communis pycom111g03120 pycom17g03630
rosa_chinensis RchiOBHm_Chr2g0170331
rosa_laevigata RLG00000021946
rosa_multiflora Rmu_sc0012445.1_g000004
rosa_roxburghii Rroxscaffold_2G00081430
rosa_rugosa Rorug02G0548200
rosa_samantha Rh2AG619500 Rh2BG630400
rosa_wichuraiana Rw2G051320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 32
AccBSI CCGCTC 1 cut(s) 69
AciI CCGC 4 cut(s) 67, 76, 79, 429
AclWI GGATC 1 cut(s) 409
AcoI YGGCCR 1 cut(s) 292
AcyI GRCGYC 1 cut(s) 33
AfiI CCNNNNNNNGG 1 cut(s) 29
AgsI TTSAA 1 cut(s) 104
AluBI AGCT 3 cut(s) 13, 20, 95
AluI AGCT 3 cut(s) 13, 20, 95
Alw21I GWGCWC 2 cut(s) 97, 247
AlwI GGATC 1 cut(s) 409
AlwNI CAGNNNCTG 1 cut(s) 290
AoxI GGCC 1 cut(s) 292
AspLEI GCGC 1 cut(s) 35
AsuC2I CCSGG 1 cut(s) 166
AsuHPI GGTGA 3 cut(s) 55, 355, 394
BalI TGGCCA 1 cut(s) 294
BanI GGYRCC 1 cut(s) 32
BanII GRGCYC 1 cut(s) 97
Bbv12I GWGCWC 2 cut(s) 97, 247
BccI CCATC 1 cut(s) 407
BcgI CGANNNNNNTGC 2 cut(s) 137, 171
BcnI CCSGG 1 cut(s) 166
BfoI RGCGCY 1 cut(s) 36
BisI GCNGC 1 cut(s) 79
BlsI GCNGC 1 cut(s) 80
Bme1390I CCNGG 1 cut(s) 166
BmiI GGNNCC 1 cut(s) 34
BmrFI CCNGG 1 cut(s) 166
BpuEI CTTGAG 1 cut(s) 81
BpuMI CCSGG 1 cut(s) 166
BsaHI GRCGYC 1 cut(s) 33
BsaJI CCNNGG 2 cut(s) 295, 405
Bsc4I CCNNNNNNNGG 1 cut(s) 29
Bse118I RCCGGY 1 cut(s) 280
BseDI CCNNGG 2 cut(s) 295, 405
BseLI CCNNNNNNNGG 1 cut(s) 29
BseMII CTCAG 1 cut(s) 102
BseRI GAGGAG 2 cut(s) 48, 425
Bsh1285I CGRYCG 1 cut(s) 429
BshFI GGCC 1 cut(s) 294
BshNI GGYRCC 1 cut(s) 32
BsiEI CGRYCG 1 cut(s) 429
BsiHKAI GWGCWC 2 cut(s) 97, 247
BsiSI CCGG 2 cut(s) 165, 281
BslFI GGGAC 1 cut(s) 181
BslI CCNNNNNNNGG 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 181
BsmI GAATGC 1 cut(s) 347
BsnI GGCC 1 cut(s) 294
Bsp1286I GDGCHC 2 cut(s) 97, 247
Bsp143I GATC 2 cut(s) 391, 414
BspACI CCGC 4 cut(s) 67, 76, 79, 429
BspANI GGCC 1 cut(s) 294
BspCNI CTCAG 1 cut(s) 103
BspLI GGNNCC 1 cut(s) 34
BspPI GGATC 1 cut(s) 409
BspT107I GGYRCC 1 cut(s) 32
BsrBI CCGCTC 1 cut(s) 69
BsrFI RCCGGY 1 cut(s) 280
BssAI RCCGGY 1 cut(s) 280
BssECI CCNNGG 2 cut(s) 295, 405
BssMI GATC 2 cut(s) 391, 414
BssNI GRCGYC 1 cut(s) 33
BssT1I CCWWGG 2 cut(s) 295, 405
BstACI GRCGYC 1 cut(s) 33
BstC8I GCNNGC 3 cut(s) 157, 292, 353
BstDEI CTNAG 1 cut(s) 111
BstH2I RGCGCY 1 cut(s) 36
BstHHI GCGC 1 cut(s) 35
BstKTI GATC 2 cut(s) 394, 417
BstMBI GATC 2 cut(s) 391, 414
BstMCI CGRYCG 1 cut(s) 429
BstMWI GCNNNNNNNGC 1 cut(s) 75
BstNSI RCATGY 1 cut(s) 355
BstSCI CCNGG 1 cut(s) 164
BstX2I RGATCY 1 cut(s) 414
BstXI CCANNNNNNTGG 1 cut(s) 406
BstYI RGATCY 1 cut(s) 414
BsuRI GGCC 1 cut(s) 294
BtsI GCAGTG 1 cut(s) 346
BtsIMutI CAGTG 1 cut(s) 346
Cac8I GCNNGC 3 cut(s) 157, 292, 353
CaiI CAGNNNCTG 1 cut(s) 290
CfoI GCGC 1 cut(s) 35
Cfr10I RCCGGY 1 cut(s) 280
CviAII CATG 2 cut(s) 325, 352
CviJI RGCY 6 cut(s) 13, 20, 95, 290, 294, 300
CviKI_1 RGCY 6 cut(s) 13, 20, 95, 290, 294, 300
DdeI CTNAG 1 cut(s) 111
DinI GGCGCC 1 cut(s) 34
DpnI GATC 2 cut(s) 393, 416
DpnII GATC 2 cut(s) 391, 414
EaeI YGGCCR 1 cut(s) 292
Ecl136II GAGCTC 1 cut(s) 95
Eco130I CCWWGG 2 cut(s) 295, 405
Eco24I GRGCYC 1 cut(s) 97
Eco53kI GAGCTC 1 cut(s) 95
EcoICRI GAGCTC 1 cut(s) 95
EcoT14I CCWWGG 2 cut(s) 295, 405
EcoT38I GRGCYC 1 cut(s) 97
EgeI GGCGCC 1 cut(s) 34
EheI GGCGCC 1 cut(s) 34
ErhI CCWWGG 2 cut(s) 295, 405
FaeI CATG 2 cut(s) 328, 355
FaiI YATR 5 cut(s) 131, 153, 172, 326, 353
FaqI GGGAC 1 cut(s) 181
FatI CATG 2 cut(s) 324, 351
FauI CCCGC 2 cut(s) 74, 83
Fnu4HI GCNGC 1 cut(s) 79
FriOI GRGCYC 1 cut(s) 97
Fsp4HI GCNGC 1 cut(s) 79
GlaI GCGC 1 cut(s) 34
GluI GCNGC 1 cut(s) 79
HaeII RGCGCY 1 cut(s) 36
HaeIII GGCC 1 cut(s) 294
HapII CCGG 2 cut(s) 165, 281
HhaI GCGC 1 cut(s) 35
Hin1I GRCGYC 1 cut(s) 33
Hin1II CATG 2 cut(s) 328, 355
Hin6I GCGC 1 cut(s) 33
HinP1I GCGC 1 cut(s) 33
HinfI GANTC 3 cut(s) 4, 233, 422
HpaII CCGG 2 cut(s) 165, 281
HphI GGTGA 3 cut(s) 55, 355, 394
Hpy188I TCNGA 3 cut(s) 145, 396, 419
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 2 cut(s) 63, 331
HpyCH4V TGCA 1 cut(s) 351
HpyF10VI GCNNNNNNNGC 1 cut(s) 75
HpyF3I CTNAG 1 cut(s) 111
Hsp92I GRCGYC 1 cut(s) 33
Hsp92II CATG 2 cut(s) 328, 355
HspAI GCGC 1 cut(s) 33
KasI GGCGCC 1 cut(s) 32
Kzo9I GATC 2 cut(s) 391, 414
LmnI GCTCC 6 cut(s) 18, 25, 54, 74, 145, 250
LpnPI CCDG 7 cut(s) 23, 178, 276, 294, 328, 369, 381
MaeIII GTNAC 1 cut(s) 193
MalI GATC 2 cut(s) 393, 416
MbiI CCGCTC 1 cut(s) 69
MboI GATC 2 cut(s) 391, 414
MflI RGATCY 1 cut(s) 414
MhlI GDGCHC 2 cut(s) 97, 247
MlsI TGGCCA 1 cut(s) 294
MluCI AATT 1 cut(s) 87
MluNI TGGCCA 1 cut(s) 294
Mly113I GGCGCC 1 cut(s) 33
MnlI CCTC 6 cut(s) 55, 69, 126, 280, 321, 403
Mox20I TGGCCA 1 cut(s) 294
MscI TGGCCA 1 cut(s) 294
MslI CAYNNNNRTG 1 cut(s) 270
Msp20I TGGCCA 1 cut(s) 294
MspI CCGG 2 cut(s) 165, 281
MspR9I CCNGG 1 cut(s) 166
Mva1269I GAATGC 1 cut(s) 347
MwoI GCNNNNNNNGC 1 cut(s) 75
NarI GGCGCC 1 cut(s) 33
NciI CCSGG 1 cut(s) 166
NdeII GATC 2 cut(s) 391, 414
NlaIII CATG 2 cut(s) 328, 355
NlaIV GGNNCC 1 cut(s) 34
NmuCI GTSAC 1 cut(s) 193
NspI RCATGY 1 cut(s) 355
PaeI GCATGC 1 cut(s) 355
PctI GAATGC 1 cut(s) 347
PfeI GAWTC 2 cut(s) 233, 422
PfoI TCCNGGA 1 cut(s) 164
PkrI GCNGC 1 cut(s) 80
PluTI GGCGCC 1 cut(s) 36
Psp124BI GAGCTC 1 cut(s) 97
PspN4I GGNNCC 1 cut(s) 34
PstNI CAGNNNCTG 1 cut(s) 290
PsuI RGATCY 1 cut(s) 414
RseI CAYNNNNRTG 1 cut(s) 270
SacI GAGCTC 1 cut(s) 97
SatI GCNGC 1 cut(s) 79
Sau3AI GATC 2 cut(s) 391, 414
ScrFI CCNGG 1 cut(s) 166
SduI GDGCHC 2 cut(s) 97, 247
SetI ASST 4 cut(s) 15, 22, 97, 407
SfoI GGCGCC 1 cut(s) 34
SmiMI CAYNNNNRTG 1 cut(s) 270
SmlI CTYRAG 1 cut(s) 96
SmoI CTYRAG 1 cut(s) 96
SphI GCATGC 1 cut(s) 355
Sse9I AATT 1 cut(s) 87
SsiI CCGC 4 cut(s) 67, 76, 79, 429
SspDI GGCGCC 1 cut(s) 32
SstI GAGCTC 1 cut(s) 97
StyD4I CCNGG 1 cut(s) 164
StyI CCWWGG 2 cut(s) 295, 405
TaqI TCGA 2 cut(s) 91, 425
TasI AATT 1 cut(s) 87
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 2 cut(s) 233, 422
TscAI CASTG 1 cut(s) 353
TseFI GTSAC 1 cut(s) 193
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 1 cut(s) 225
TspGWI ACGGA 1 cut(s) 12
TspRI CASTG 1 cut(s) 353
XceI RCATGY 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.