MD17G1052000.v1.1

SPX domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010469
Physical Location & Seq
Forward (+)
4091091 .. 4093213
2123 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1052000.v1.1.491

Sequence Viewer

Length: 1101 bp
ATGAAATTCGGGAAGGAGTTCAGGACCCACCTGGAAGAGACACTCCCGGAGTGGAGGGACAAGTTCCTTTGCTACAAGCTATTGAAGAAGCTCCTCAAGGGCTTGCCCGCCACCGCGGCCACTATCGCCGCGGTGGATTCCCTCCCTCGCCACCTCGAGTTTCAGCTGGCATATGCGTATCCGGCTGCTGGTGATGGAGGTGGCGGTGGCGGGTTCCATCCTTTAGCGGGACTCCAATATTGGTTCGTTGCGATTTTGAATGAGGAGCTGTATAAGTTAAACGATTTCTATATGGATAAGGAGGAGGATTTCGTTATACGATTCCAGGAGCTGAAGGGAAGAATCGAGATCGTCAGGGAAAGGAGCAGCAGGGGTGGAGTTTTTACATCAGAGACCGAGTTCAGCAAAGAAGTGATGGACATCCGTAAGGACTTTGTCACCATTCACGGGGAGATGGTGCTTCTCAAAAGTTATAGCTCGTTGAATTTTACGGGTACAGCAGCCACTTTTCACCTTCCATATTTAACCTGTATTGTACAACTGTTAGCATCAGTGAATTTCTTCATTCTATATCTGCGTTATCTATTGCTAAAATCAGCTGTGTCTTATGTCTCAACAGGTCTTGTCAAAATTCTAAAGAAGTACGATAAACGAACTGGAGGAGTATTGCGTTTATGTTTCACGCAACTAGCTCACCAGCCTTTCTTTACAACAGAACTTCTGACGAGGCTAGTCAGTGAATGCGAGGCAAATCTCGAGCTTCTCTTCCCATTGGAAGCAGAAGTCATTGAAGGTACACCAACCACACCAGATGAAACCACCCCAGACAGAAGTCATTCAAACCTACAATCGAATCATTCGGCAAATATTGCATCTGAGACACCTTCAAACATGGAGGCAAACATGGATATATATCGTGGAACCGTTGCTGCAATGAAGGCCATTCAAGGCCTTCAAAAACCAAGCTCCACTTCCGGTCCATGGTCGCTTTCAAATCACTTTAGAAGCCAGGACGAGGAGAGCACTGGTGCTGTCACTGAAAACTCTACTTCAAACTCTGTAGCGAGCTGGCAAAGCGAAGATGAGGATGACCAGTTATAG

Protein Analysis

367

Amino Acids

41.08

Weight (kDa)

5.39

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPX PF03105 1 - 35 9.8e-09 SPX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 116, 131
AciI CCGC 8 cut(s) 108, 114, 116, 129, 131, 204, 210, 227
AcoI YGGCCR 1 cut(s) 117
AcsI RAATTY 4 cut(s) 5, 484, 556, 630
AcuI CTGAAG 1 cut(s) 353
AfaI GTAC 4 cut(s) 496, 537, 644, 796
AfiI CCNNNNNNNGG 5 cut(s) 188, 227, 447, 981, 1015
AjnI CCWGG 3 cut(s) 30, 324, 1008
AjuI GAANNNNNNNTTGG 2 cut(s) 955, 987
Alw21I GWGCWC 1 cut(s) 1025
Alw26I GTCTC 4 cut(s) 32, 386, 616, 872
AlwNI CAGNNNCTG 1 cut(s) 331
Ama87I CYCGRG 2 cut(s) 155, 755
AoxI GGCC 3 cut(s) 117, 939, 949
ApeKI GCWGC 4 cut(s) 185, 366, 500, 929
ApoI RAATTY 4 cut(s) 5, 484, 556, 630
Asp700I GAANNNNTTC 3 cut(s) 17, 560, 835
AspS9I GGNCC 2 cut(s) 24, 977
AsuC2I CCSGG 1 cut(s) 47
AsuHPI GGTGA 4 cut(s) 203, 430, 503, 686
AvaI CYCGRG 2 cut(s) 155, 755
AvaII GGWCC 2 cut(s) 24, 977
Bbv12I GWGCWC 1 cut(s) 1025
BbvI GCAGC 4 cut(s) 172, 378, 512, 916
BccI CCATC 4 cut(s) 188, 225, 409, 448
BciT130I CCWGG 3 cut(s) 32, 326, 1010
BciVI GTATCC 1 cut(s) 189
BcnI CCSGG 1 cut(s) 47
BcoDI GTCTC 4 cut(s) 32, 386, 616, 872
BfaI CTAG 2 cut(s) 689, 731
BfmI CTRYAG 1 cut(s) 1059
BfuI GTATCC 1 cut(s) 189
BglI GCCNNNNNGGC 1 cut(s) 116
BisI GCNGC 6 cut(s) 117, 129, 186, 367, 501, 930
BlsI GCNGC 6 cut(s) 118, 130, 187, 368, 502, 931
Bme1390I CCNGG 4 cut(s) 32, 47, 326, 1010
Bme18I GGWCC 2 cut(s) 24, 977
BmeT110I CYCGRG 2 cut(s) 155, 755
BmgT120I GGNCC 2 cut(s) 24, 977
BmiI GGNNCC 3 cut(s) 26, 215, 922
BmrFI CCNGG 4 cut(s) 32, 47, 326, 1010
BmsI GCATC 2 cut(s) 557, 881
BoxI GACNNNNGTC 1 cut(s) 831
BpmI CTGGAG 1 cut(s) 678
BpuEI CTTGAG 1 cut(s) 80
BpuMI CCSGG 1 cut(s) 47
BsaBI GATNNNNATC 2 cut(s) 419, 912
BsaI GGTCTC 1 cut(s) 386
BsaJI CCNNGG 3 cut(s) 114, 129, 980
BsaWI WCCGGW 1 cut(s) 974
Bsc4I CCNNNNNNNGG 5 cut(s) 188, 227, 447, 981, 1015
Bse1I ACTGG 3 cut(s) 661, 1030, 1093
Bse3DI GCAATG 1 cut(s) 939
Bse8I GATNNNNATC 2 cut(s) 419, 912
BseBI CCWGG 3 cut(s) 32, 326, 1010
BseDI CCNNGG 3 cut(s) 114, 129, 980
BseGI GGATG 3 cut(s) 217, 420, 1093
BseJI GATNNNNATC 2 cut(s) 419, 912
BseLI CCNNNNNNNGG 5 cut(s) 188, 227, 447, 981, 1015
BseMI GCAATG 1 cut(s) 939
BseMII CTCAG 1 cut(s) 867
BseNI ACTGG 3 cut(s) 661, 1030, 1093
BseRI GAGGAG 5 cut(s) 83, 278, 317, 675, 1031
BseXI GCAGC 4 cut(s) 172, 378, 512, 916
Bsh1236I CGCG 2 cut(s) 116, 131
BshFI GGCC 3 cut(s) 119, 941, 951
BsiHKAI GWGCWC 1 cut(s) 1025
BsiHKCI CYCGRG 2 cut(s) 155, 755
BsiSI CCGG 3 cut(s) 47, 182, 975
BslFI GGGAC 2 cut(s) 71, 243
BslI CCNNNNNNNGG 5 cut(s) 188, 227, 447, 981, 1015
BsmAI GTCTC 4 cut(s) 32, 386, 616, 872
BsmFI GGGAC 2 cut(s) 71, 243
BsmI GAATGC 1 cut(s) 746
BsnI GGCC 3 cut(s) 119, 941, 951
Bso31I GGTCTC 1 cut(s) 386
BsoBI CYCGRG 2 cut(s) 155, 755
Bsp1286I GDGCHC 1 cut(s) 1025
Bsp1407I TGTACA 1 cut(s) 535
Bsp143I GATC 1 cut(s) 348
Bsp19I CCATGG 1 cut(s) 980
BspACI CCGC 8 cut(s) 108, 114, 116, 129, 131, 204, 210, 227
BspANI GGCC 3 cut(s) 119, 941, 951
BspCNI CTCAG 1 cut(s) 868
BspFNI CGCG 2 cut(s) 116, 131
BspLI GGNNCC 3 cut(s) 26, 215, 922
BspTNI GGTCTC 1 cut(s) 386
BsrDI GCAATG 1 cut(s) 939
BsrGI TGTACA 1 cut(s) 535
BsrI ACTGG 3 cut(s) 661, 1030, 1093
BssECI CCNNGG 3 cut(s) 114, 129, 980
BssMI GATC 1 cut(s) 348
BssT1I CCWWGG 1 cut(s) 980
Bst2UI CCWGG 3 cut(s) 32, 326, 1010
Bst4CI ACNGT 2 cut(s) 543, 925
Bst6I CTCTTC 2 cut(s) 30, 770
BstAPI GCANNNNNTGC 1 cut(s) 869
BstAUI TGTACA 1 cut(s) 535
BstC8I GCNNGC 5 cut(s) 104, 108, 168, 1066, 1070
BstDEI CTNAG 1 cut(s) 876
BstDSI CCRYGG 3 cut(s) 114, 129, 980
BstF5I GGATG 3 cut(s) 217, 420, 1093
BstFNI CGCG 2 cut(s) 116, 131
BstKTI GATC 1 cut(s) 351
BstMAI GTCTC 4 cut(s) 32, 386, 616, 872
BstMBI GATC 1 cut(s) 348
BstMWI GCNNNNNNNGC 6 cut(s) 116, 125, 182, 869, 938, 1074
BstNI CCWGG 3 cut(s) 32, 326, 1010
BstPAI GACNNNNGTC 1 cut(s) 831
BstSCI CCNGG 4 cut(s) 30, 45, 324, 1008
BstSFI CTRYAG 1 cut(s) 1059
BstUI CGCG 2 cut(s) 116, 131
BstV1I GCAGC 4 cut(s) 172, 378, 512, 916
BsuI GTATCC 1 cut(s) 189
BsuRI GGCC 3 cut(s) 119, 941, 951
BtgI CCRYGG 3 cut(s) 114, 129, 980
BtsCI GGATG 3 cut(s) 217, 420, 1093
BtsIMutI CAGTG 4 cut(s) 558, 742, 1023, 1035
Cac8I GCNNGC 5 cut(s) 104, 108, 168, 1066, 1070
CaiI CAGNNNCTG 1 cut(s) 331
Cfr13I GGNCC 2 cut(s) 24, 977
Cfr42I CCGCGG 2 cut(s) 117, 132
Csp6I GTAC 4 cut(s) 495, 536, 643, 795
CviAII CATG 3 cut(s) 892, 904, 981
CviQI GTAC 4 cut(s) 495, 536, 643, 795
DdeI CTNAG 1 cut(s) 876
DpnI GATC 1 cut(s) 350
DpnII GATC 1 cut(s) 348
EaeI YGGCCR 1 cut(s) 117
Eam1104I CTCTTC 2 cut(s) 30, 770
EarI CTCTTC 2 cut(s) 30, 770
Eco130I CCWWGG 1 cut(s) 980
Eco147I AGGCCT 1 cut(s) 951
Eco31I GGTCTC 1 cut(s) 386
Eco47I GGWCC 2 cut(s) 24, 977
Eco57I CTGAAG 1 cut(s) 353
Eco88I CYCGRG 2 cut(s) 155, 755
EcoO109I RGGNCCY 1 cut(s) 24
EcoRII CCWGG 3 cut(s) 30, 324, 1008
EcoT14I CCWWGG 1 cut(s) 980
ErhI CCWWGG 1 cut(s) 980
FaeI CATG 3 cut(s) 895, 907, 984
FalI AAGNNNNNCTT 2 cut(s) 955, 987
FaqI GGGAC 2 cut(s) 71, 243
FatI CATG 3 cut(s) 891, 903, 980
FauI CCCGC 3 cut(s) 115, 203, 220
FauNDI CATATG 1 cut(s) 172
Fnu4HI GCNGC 6 cut(s) 117, 129, 186, 367, 501, 930
FokI GGATG 2 cut(s) 204, 407
Fsp4HI GCNGC 6 cut(s) 117, 129, 186, 367, 501, 930
FspBI CTAG 2 cut(s) 689, 731
GluI GCNGC 6 cut(s) 117, 129, 186, 367, 501, 930
GsuI CTGGAG 1 cut(s) 678
HaeIII GGCC 3 cut(s) 119, 941, 951
HapII CCGG 3 cut(s) 47, 182, 975
Hin1II CATG 3 cut(s) 895, 907, 984
HinfI GANTC 5 cut(s) 137, 231, 321, 342, 853
HpaII CCGG 3 cut(s) 47, 182, 975
HphI GGTGA 4 cut(s) 203, 430, 503, 686
Hpy166II GTNNAC 1 cut(s) 797
Hpy188I TCNGA 3 cut(s) 391, 723, 877
Hpy188III TCNNGA 4 cut(s) 10, 22, 346, 755
Hpy8I GTNNAC 1 cut(s) 797
HpyAV CCTTC 7 cut(s) 7, 328, 524, 785, 894, 931, 962
HpyCH4III ACNGT 2 cut(s) 543, 925
HpyCH4V TGCA 2 cut(s) 872, 932
HpyF10VI GCNNNNNNNGC 6 cut(s) 116, 125, 182, 869, 938, 1074
HpyF3I CTNAG 1 cut(s) 876
Hsp92II CATG 3 cut(s) 895, 907, 984
KspI CCGCGG 2 cut(s) 117, 132
Kzo9I GATC 1 cut(s) 348
LmnI GCTCC 5 cut(s) 96, 265, 328, 363, 971
Lsp1109I GCAGC 4 cut(s) 172, 378, 512, 916
LweI GCATC 2 cut(s) 557, 881
MaeI CTAG 2 cut(s) 689, 731
MaeIII GTNAC 2 cut(s) 436, 1033
MalI GATC 1 cut(s) 350
MboI GATC 1 cut(s) 348
MboII GAAGA 6 cut(s) 47, 97, 351, 553, 757, 1091
MhlI GDGCHC 1 cut(s) 1025
MluCI AATT 4 cut(s) 5, 484, 556, 630
MlyI GAGTC 1 cut(s) 225
MroXI GAANNNNTTC 3 cut(s) 17, 560, 835
MseI TTAA 2 cut(s) 278, 524
MspA1I CMGCKG 4 cut(s) 116, 131, 166, 599
MspI CCGG 3 cut(s) 47, 182, 975
MspR9I CCNGG 4 cut(s) 32, 47, 326, 1010
Mva1269I GAATGC 1 cut(s) 746
MvaI CCWGG 3 cut(s) 32, 326, 1010
MvnI CGCG 2 cut(s) 116, 131
MwoI GCNNNNNNNGC 6 cut(s) 116, 125, 182, 869, 938, 1074
NciI CCSGG 1 cut(s) 47
NcoI CCATGG 1 cut(s) 980
NdeI CATATG 1 cut(s) 172
NdeII GATC 1 cut(s) 348
NlaIII CATG 3 cut(s) 895, 907, 984
NlaIV GGNNCC 3 cut(s) 26, 215, 922
NmuCI GTSAC 2 cut(s) 436, 1033
PaeR7I CTCGAG 2 cut(s) 155, 755
PceI AGGCCT 1 cut(s) 951
PctI GAATGC 1 cut(s) 746
PdmI GAANNNNTTC 3 cut(s) 17, 560, 835
PfeI GAWTC 4 cut(s) 137, 321, 342, 853
PflFI GACNNNGTC 1 cut(s) 434
PfoI TCCNGGA 2 cut(s) 45, 324
PkrI GCNGC 6 cut(s) 118, 130, 187, 368, 502, 931
PleI GAGTC 1 cut(s) 225
PpsI GAGTC 1 cut(s) 225
PpuMI RGGWCCY 1 cut(s) 24
PshAI GACNNNNGTC 1 cut(s) 831
Psp5II RGGWCCY 1 cut(s) 24
Psp6I CCWGG 3 cut(s) 30, 324, 1008
PspGI CCWGG 3 cut(s) 30, 324, 1008
PspN4I GGNNCC 3 cut(s) 26, 215, 922
PspPI GGNCC 2 cut(s) 24, 977
PspPPI RGGWCCY 1 cut(s) 24
PspXI VCTCGAGB 1 cut(s) 155
PstNI CAGNNNCTG 1 cut(s) 331
PsyI GACNNNGTC 1 cut(s) 434
PvuII CAGCTG 2 cut(s) 166, 599
RsaI GTAC 4 cut(s) 496, 537, 644, 796
RsaNI GTAC 4 cut(s) 495, 536, 643, 795
SacII CCGCGG 2 cut(s) 117, 132
SaqAI TTAA 2 cut(s) 278, 524
SatI GCNGC 6 cut(s) 117, 129, 186, 367, 501, 930
Sau3AI GATC 1 cut(s) 348
Sau96I GGNCC 2 cut(s) 24, 977
SchI GAGTC 1 cut(s) 225
ScrFI CCNGG 4 cut(s) 32, 47, 326, 1010
SduI GDGCHC 1 cut(s) 1025
SfaNI GCATC 2 cut(s) 557, 881
SfcI CTRYAG 1 cut(s) 1059
Sfr274I CTCGAG 2 cut(s) 155, 755
Sfr303I CCGCGG 2 cut(s) 117, 132
SgrBI CCGCGG 2 cut(s) 117, 132
SinI GGWCC 2 cut(s) 24, 977
SlaI CTCGAG 2 cut(s) 155, 755
SmlI CTYRAG 3 cut(s) 95, 155, 755
SmoI CTYRAG 3 cut(s) 95, 155, 755
Sse9I AATT 4 cut(s) 5, 484, 556, 630
SseBI AGGCCT 1 cut(s) 951
SsiI CCGC 8 cut(s) 108, 114, 116, 129, 131, 204, 210, 227
SspI AATATT 2 cut(s) 239, 868
SspMI CTAG 2 cut(s) 689, 731
StuI AGGCCT 1 cut(s) 951
StyD4I CCNGG 4 cut(s) 30, 45, 324, 1008
StyI CCWWGG 1 cut(s) 980
TaaI ACNGT 2 cut(s) 543, 925
TaqI TCGA 4 cut(s) 156, 345, 756, 851
TaqII GACCGA 1 cut(s) 410
TasI AATT 4 cut(s) 5, 484, 556, 630
TatI WGTACW 1 cut(s) 535
TauI GCSGC 2 cut(s) 119, 131
TfiI GAWTC 4 cut(s) 137, 321, 342, 853
Tru1I TTAA 2 cut(s) 278, 524
Tru9I TTAA 2 cut(s) 278, 524
TscAI CASTG 4 cut(s) 558, 742, 1030, 1042
TseFI GTSAC 2 cut(s) 436, 1033
TseI GCWGC 4 cut(s) 185, 366, 500, 929
Tsp45I GTSAC 2 cut(s) 436, 1033
TspDTI ATGAA 4 cut(s) 17, 553, 828, 950
TspGWI ACGGA 1 cut(s) 413
TspRI CASTG 4 cut(s) 558, 742, 1030, 1042
Tth111I GACNNNGTC 1 cut(s) 434
VpaK11BI GGWCC 2 cut(s) 24, 977
XapI RAATTY 4 cut(s) 5, 484, 556, 630
XhoI CTCGAG 2 cut(s) 155, 755
XmnI GAANNNNTTC 3 cut(s) 17, 560, 835
XspI CTAG 2 cut(s) 689, 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.