Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0006551

Physical Location & Seq

Genomic Coordinates Forward (+)

6877476 .. 6881284

Gene Structure 3809 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1082300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1302 bp

ATGAGGAAGTATGTGAACCATCACTCAGCAAGGGTGATTGGTGATAGGAATTGGGTTATTCCATTCATTACTAGCTTTATCATATTCATTACCCTCTATTTCTCTGCCATTTCCGGGCTATTTACCTCCCCACACGATGGAGAGAATTTGTCATTCGACATTATTCCCTTCGCAAAATCAGATGACACAGGTGGGTATTATGTCGAATCTGATTTGAAAACATCATTGGACACCAACGGGAGTTCCAAAATTGGGGCACCTAGATTTGCGTATCTTATATCGGGCACAAGGGGTGATAGTCATAGGATGATGAGGACTTTAAGCGCGGTGTATCATCCAAGAAATCAATATATCCTGCATTTGGATCTTGAGGCTCCGCCGCGTGAAAGGTTGGAATTAGCAAGTTTAGTGAGGGCTGATCTTACTTTTCGCGAAGTGGAGAATGTGCGTGTTATGTCTCAATCCAATTTGGTGACTTATAAGGGCCCTACGATGATTGCTTGTACTCTGCAAGCCATTTCCATTCTATTAAAGGAGAGCTCAGAGTGGGACTGGTTTATAAACCTCAGTGCATCAGATTATCCTCTCATGACACAAGATGATTTGCTTCATGCTTTCTCCAATCTTTCCAGAAATATCAACTTTATTGAACATATGCAGATCACTGGATGGAAACTGAACCAAAGAGCAAAACCAATCATAATTGATCCAGGCCTGTACTTATCAAAAAAGTCTGACCTTGCACTAACTACTCAGCGCCGATCACTTCCTACATCATTCAAGTTGTTTACAGGTTCAGCGTGGGTAATGCTAACGCGATCATTTCTTGAGTATTGTATATGGGGATGGGATAACCTCCCACGCACGATCCTTATGTACTACACAAATTTTGTTTCTTCTCCGGAAGGCTATTTTCATACTGTAATTTGCAACAACGAGGAATATCGCCACACCGCAATAAGTCACGATCTCCACTACATTGCTTGGGACAGACCACCTAAGCAGCATCCCATCTCTTTATCAATGAAGGACTTTGACAAAATGGTAAAGAGCAACGCTCCCTTTGCTCGAAAGTTTGCAAAGGATGACCCAGTGTTGGACAAAATTGATAAAGAGCTTCTAGGTCGCACAAGCCGGTTTGCGCCTGGTGGGTGGTGCATAGGCAGCCCTGAAGGCGGGGCTGACCCATGCTCCGTGCGTGGTAATGATTCAGTGTTTAAGCCAGGTCCTGGTTCGGAGAGGTTCCAAGAGCTGCTTCGTACACTATTATCCGAAGATTTTCAAAGAAAGCAATGTACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0006029 GO:0006807 GO:0007275 GO:0007399 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010033 GO:0010721 GO:0010975 GO:0010977 GO:0012505 GO:0016020 GO:0019538 GO:0022008 GO:0030154 GO:0030166 GO:0031090 GO:0031344 GO:0031345 GO:0031984 GO:0032101 GO:0032102 GO:0032501 GO:0032502 GO:0034097 GO:0034645 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045595 GO:0045596 GO:0045664 GO:0045665 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048679 GO:0048681 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050768 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051128 GO:0051129 GO:0051239 GO:0051241 GO:0051960 GO:0051961 GO:0060284 GO:0065007 GO:0070555 GO:0070570 GO:0070571 GO:0071704 GO:0080134 GO:0080135 GO:0098588 GO:0098791 GO:0120035 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1903034 GO:1903035 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Branch

Protein Analysis

434

Amino Acids

49.45

Weight (kDa)

8.12

Isoelectric Point (pI)

33.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Branch	PF02485	89 - 348	6.4e-78	Core-2/I-Branching enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006551)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03520 AT1G03520 AT4G03340 AT4G03340
fragaria_vesca	FvH4_6g44880
malus_domestica	MD09G1092300.v1.1 MD17G1082300.v1.1
prunus_persica	Prupe.3G232800_v2.0.a1 Prupe.3G232800_v2.0.a1 Prupe.3G232800_v2.0.a1 Prupe.3G232800_v2.0.a1 Prupe.3G232800_v2.0.a1 Prupe.3G232800_v2.0.a1
pyrus_communis	pycom17g08040 pycom17g27370
rosa_chinensis	RchiOBHm_Chr2g0162071 RchiOBHm_Chr3g0466861
rosa_laevigata	RLG00000021320
rosa_multiflora	Rmu_sc0005601.1_g000006
rosa_roxburghii	Rroxscaffold_2G00088330
rosa_rugosa	Rorug02G0494400
rosa_samantha	Rh2AG560600 Rh2BG573900 Rh2CG544200 Rh2DG583900
rosa_wichuraiana	Rw2G046430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	480, 560
AccB1I	GGYRCC	1 cut(s)	256
AccB7I	CCANNNNNTGG	2 cut(s)	137, 1229
AccII	CGCG	4 cut(s)	326, 382, 432, 817
AccIII	TCCGGA	1 cut(s)	901
AciI	CCGC	5 cut(s)	326, 377, 380, 954, 1176
AclWI	GGATC	3 cut(s)	372, 701, 862
AcsI	RAATTY	2 cut(s)	145, 886
AcuI	CTGAAG	1 cut(s)	1191
AfaI	GTAC	5 cut(s)	505, 719, 878, 1261, 1297
AfiI	CCNNNNNNNGG	7 cut(s)	114, 137, 252, 361, 1096, 1175, 1229
AgsI	TTSAA	4 cut(s)	217, 650, 781, 1283
AjnI	CCWGG	4 cut(s)	709, 1144, 1222, 1228
AjuI	GAANNNNNNNTTGG	4 cut(s)	209, 241, 1239, 1271
AluBI	AGCT	4 cut(s)	75, 540, 1117, 1252
AluI	AGCT	4 cut(s)	75, 540, 1117, 1252
Alw21I	GWGCWC	1 cut(s)	542
Alw26I	GTCTC	1 cut(s)	462
AlwI	GGATC	3 cut(s)	372, 701, 862
AlwNI	CAGNNNCTG	1 cut(s)	1229
Aor13HI	TCCGGA	1 cut(s)	901
AoxI	GGCC	2 cut(s)	484, 712
ApaI	GGGCCC	1 cut(s)	488
ApeKI	GCWGC	3 cut(s)	1003, 1164, 1252
ApoI	RAATTY	2 cut(s)	145, 886
Asp700I	GAANNNNTTC	1 cut(s)	1278
AspLEI	GCGC	3 cut(s)	326, 759, 1144
AspS9I	GGNCC	3 cut(s)	484, 485, 1226
AsuC2I	CCSGG	1 cut(s)	115
AsuHPI	GGTGA	4 cut(s)	46, 53, 305, 484
AvaII	GGWCC	1 cut(s)	1226
BaeGI	GKGCMC	3 cut(s)	259, 287, 488
BanI	GGYRCC	1 cut(s)	256
BanII	GRGCYC	2 cut(s)	488, 542
Bbv12I	GWGCWC	1 cut(s)	542
BbvI	GCAGC	3 cut(s)	1015, 1176, 1239
BccI	CCATC	5 cut(s)	27, 131, 663, 840, 1019
BciT130I	CCWGG	4 cut(s)	711, 1146, 1224, 1230
BcnI	CCSGG	1 cut(s)	115
BcoDI	GTCTC	1 cut(s)	462
BfaI	CTAG	3 cut(s)	72, 261, 1121
BfoI	RGCGCY	1 cut(s)	760
BglI	GCCNNNNNGGC	1 cut(s)	1173
BisI	GCNGC	4 cut(s)	380, 1004, 1165, 1253
BlsI	GCNGC	4 cut(s)	381, 1005, 1166, 1254
Bme1390I	CCNGG	5 cut(s)	115, 711, 1146, 1224, 1230
Bme18I	GGWCC	1 cut(s)	1226
BmgT120I	GGNCC	3 cut(s)	484, 485, 1226
BmiI	GGNNCC	4 cut(s)	258, 375, 486, 1244
BmrFI	CCNGG	5 cut(s)	115, 711, 1146, 1224, 1230
BmrI	ACTGGG	1 cut(s)	1085
BmsI	GCATC	2 cut(s)	581, 1015
BmuI	ACTGGG	1 cut(s)	1085
BplI	GAGNNNNNCTC	2 cut(s)	1042, 1074
Bpu10I	CCTNAGC	1 cut(s)	999
BpuEI	CTTGAG	2 cut(s)	389, 848
BpuMI	CCSGG	1 cut(s)	115
BsaWI	WCCGGW	1 cut(s)	901
BsaXI	ACNNNNNCTCC	2 cut(s)	1175, 1205
Bsc4I	CCNNNNNNNGG	7 cut(s)	114, 137, 252, 361, 1096, 1175, 1229
Bse118I	RCCGGY	1 cut(s)	1134
Bse1I	ACTGG	3 cut(s)	557, 670, 1091
Bse3DI	GCAATG	2 cut(s)	978, 1298
BseAI	TCCGGA	1 cut(s)	901
BseBI	CCWGG	4 cut(s)	711, 1146, 1224, 1230
BseGI	GGATG	6 cut(s)	312, 334, 674, 851, 1006, 1090
BseLI	CCNNNNNNNGG	7 cut(s)	114, 137, 252, 361, 1096, 1175, 1229
BseMI	GCAATG	2 cut(s)	978, 1298
BseMII	CTCAG	4 cut(s)	39, 555, 580, 767
BseNI	ACTGG	3 cut(s)	557, 670, 1091
BseSI	GKGCMC	3 cut(s)	259, 287, 488
BseXI	GCAGC	3 cut(s)	1015, 1176, 1239
Bsh1236I	CGCG	4 cut(s)	326, 382, 432, 817
BshFI	GGCC	2 cut(s)	486, 714
BshNI	GGYRCC	1 cut(s)	256
BsiHKAI	GWGCWC	1 cut(s)	542
BsiSI	CCGG	3 cut(s)	114, 902, 1135
BslFI	GGGAC	2 cut(s)	563, 1001
BslI	CCNNNNNNNGG	7 cut(s)	114, 137, 252, 361, 1096, 1175, 1229
BsmAI	GTCTC	1 cut(s)	462
BsmFI	GGGAC	2 cut(s)	563, 1001
BsnI	GGCC	2 cut(s)	486, 714
Bsp120I	GGGCCC	1 cut(s)	484
Bsp1286I	GDGCHC	4 cut(s)	259, 287, 488, 542
Bsp13I	TCCGGA	1 cut(s)	901
Bsp1407I	TGTACA	1 cut(s)	1295
Bsp143I	GATC	8 cut(s)	364, 418, 660, 706, 761, 818, 867, 967
Bsp68I	TCGCGA	1 cut(s)	432
BspACI	CCGC	5 cut(s)	326, 377, 380, 954, 1176
BspANI	GGCC	2 cut(s)	486, 714
BspCNI	CTCAG	4 cut(s)	38, 554, 579, 766
BspEI	TCCGGA	1 cut(s)	901
BspFNI	CGCG	4 cut(s)	326, 382, 432, 817
BspHI	TCATGA	1 cut(s)	588
BspLI	GGNNCC	4 cut(s)	258, 375, 486, 1244
BspPI	GGATC	3 cut(s)	372, 701, 862
BspT107I	GGYRCC	1 cut(s)	256
BsrDI	GCAATG	2 cut(s)	978, 1298
BsrFI	RCCGGY	1 cut(s)	1134
BsrGI	TGTACA	1 cut(s)	1295
BsrI	ACTGG	3 cut(s)	557, 670, 1091
BssAI	RCCGGY	1 cut(s)	1134
BssMI	GATC	8 cut(s)	364, 418, 660, 706, 761, 818, 867, 967
Bst2UI	CCWGG	4 cut(s)	711, 1146, 1224, 1230
Bst4CI	ACNGT	1 cut(s)	922
BstAUI	TGTACA	1 cut(s)	1295
BstC8I	GCNNGC	1 cut(s)	513
BstDEI	CTNAG	5 cut(s)	25, 541, 566, 753, 999
BstF5I	GGATG	6 cut(s)	312, 334, 674, 851, 1006, 1090
BstFNI	CGCG	4 cut(s)	326, 382, 432, 817
BstH2I	RGCGCY	1 cut(s)	760
BstHHI	GCGC	3 cut(s)	326, 759, 1144
BstKTI	GATC	8 cut(s)	367, 421, 663, 709, 764, 821, 870, 970
BstMAI	GTCTC	1 cut(s)	462
BstMBI	GATC	8 cut(s)	364, 418, 660, 706, 761, 818, 867, 967
BstMWI	GCNNNNNNNGC	3 cut(s)	1064, 1164, 1173
BstNI	CCWGG	4 cut(s)	711, 1146, 1224, 1230
BstSCI	CCNGG	5 cut(s)	113, 709, 1144, 1222, 1228
BstSLI	GKGCMC	3 cut(s)	259, 287, 488
BstUI	CGCG	4 cut(s)	326, 382, 432, 817
BstV1I	GCAGC	3 cut(s)	1015, 1176, 1239
BstX2I	RGATCY	1 cut(s)	364
BstYI	RGATCY	1 cut(s)	364
BsuRI	GGCC	2 cut(s)	486, 714
BtsCI	GGATG	6 cut(s)	312, 334, 674, 851, 1006, 1090
BtsIMutI	CAGTG	4 cut(s)	574, 663, 1098, 1218
BtuMI	TCGCGA	1 cut(s)	432
Cac8I	GCNNGC	1 cut(s)	513
CaiI	CAGNNNCTG	1 cut(s)	1229
CciI	TCATGA	1 cut(s)	588
CfoI	GCGC	3 cut(s)	326, 759, 1144
Cfr10I	RCCGGY	1 cut(s)	1134
Cfr13I	GGNCC	3 cut(s)	484, 485, 1226
Csp6I	GTAC	5 cut(s)	504, 718, 877, 1260, 1296
CviAII	CATG	4 cut(s)	589, 611, 1188, 1299
CviQI	GTAC	5 cut(s)	504, 718, 877, 1260, 1296
DdeI	CTNAG	5 cut(s)	25, 541, 566, 753, 999
DpnI	GATC	8 cut(s)	366, 420, 662, 708, 763, 820, 869, 969
DpnII	GATC	8 cut(s)	364, 418, 660, 706, 761, 818, 867, 967
EciI	GGCGGA	1 cut(s)	366
Ecl136II	GAGCTC	1 cut(s)	540
Eco147I	AGGCCT	1 cut(s)	714
Eco24I	GRGCYC	2 cut(s)	488, 542
Eco47I	GGWCC	1 cut(s)	1226
Eco53kI	GAGCTC	1 cut(s)	540
Eco57I	CTGAAG	1 cut(s)	1191
EcoICRI	GAGCTC	1 cut(s)	540
EcoO109I	RGGNCCY	3 cut(s)	484, 485, 1226
EcoRII	CCWGG	4 cut(s)	709, 1144, 1222, 1228
EcoT38I	GRGCYC	2 cut(s)	488, 542
FaeI	CATG	4 cut(s)	592, 614, 1191, 1302
FalI	AAGNNNNNCTT	2 cut(s)	1239, 1271
FaqI	GGGAC	2 cut(s)	563, 1001
FatI	CATG	4 cut(s)	588, 610, 1187, 1298
FauI	CCCGC	1 cut(s)	1169
FauNDI	CATATG	1 cut(s)	654
Fnu4HI	GCNGC	4 cut(s)	380, 1004, 1165, 1253
FokI	GGATG	6 cut(s)	319, 321, 681, 858, 993, 1097
FriOI	GRGCYC	2 cut(s)	488, 542
Fsp4HI	GCNGC	4 cut(s)	380, 1004, 1165, 1253
FspBI	CTAG	3 cut(s)	72, 261, 1121
GlaI	GCGC	3 cut(s)	325, 758, 1143
GluI	GCNGC	4 cut(s)	380, 1004, 1165, 1253
HaeII	RGCGCY	1 cut(s)	760
HaeIII	GGCC	2 cut(s)	486, 714
HapII	CCGG	3 cut(s)	114, 902, 1135
HhaI	GCGC	3 cut(s)	326, 759, 1144
Hin1II	CATG	4 cut(s)	592, 614, 1191, 1302
Hin6I	GCGC	3 cut(s)	324, 757, 1142
HinP1I	GCGC	3 cut(s)	324, 757, 1142
HinfI	GANTC	2 cut(s)	206, 1208
HpaII	CCGG	3 cut(s)	114, 902, 1135
HphI	GGTGA	4 cut(s)	46, 53, 305, 484
Hpy166II	GTNNAC	3 cut(s)	16, 789, 1262
Hpy188I	TCNGA	7 cut(s)	181, 211, 544, 577, 736, 1237, 1273
Hpy188III	TCNNGA	7 cut(s)	368, 431, 589, 630, 827, 902, 965
Hpy8I	GTNNAC	3 cut(s)	16, 789, 1262
HpyAV	CCTTC	4 cut(s)	178, 899, 1021, 1166
HpyCH4III	ACNGT	1 cut(s)	922
HpyCH4V	TGCA	8 cut(s)	358, 511, 572, 658, 743, 930, 1079, 1158
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1064, 1164, 1173
HpyF3I	CTNAG	5 cut(s)	25, 541, 566, 753, 999
Hsp92II	CATG	4 cut(s)	592, 614, 1191, 1302
HspAI	GCGC	3 cut(s)	324, 757, 1142
Kpn2I	TCCGGA	1 cut(s)	901
Kzo9I	GATC	8 cut(s)	364, 418, 660, 706, 761, 818, 867, 967
LmnI	GCTCC	3 cut(s)	379, 1063, 1196
Lsp1109I	GCAGC	3 cut(s)	1015, 1176, 1239
LweI	GCATC	2 cut(s)	581, 1015
MaeI	CTAG	3 cut(s)	72, 261, 1121
MaeIII	GTNAC	2 cut(s)	472, 962
MalI	GATC	8 cut(s)	366, 420, 662, 708, 763, 820, 869, 969
MboI	GATC	8 cut(s)	364, 418, 660, 706, 761, 818, 867, 967
MboII	GAAGA	2 cut(s)	888, 1286
MflI	RGATCY	1 cut(s)	364
MhlI	GDGCHC	4 cut(s)	259, 287, 488, 542
MluCI	AATT	9 cut(s)	49, 145, 249, 395, 466, 702, 886, 924, 1104
MmeI	TCCRAC	2 cut(s)	372, 1077
MroI	TCCGGA	1 cut(s)	901
MroXI	GAANNNNTTC	1 cut(s)	1278
MseI	TTAA	3 cut(s)	320, 530, 1218
MspI	CCGG	3 cut(s)	114, 902, 1135
MspR9I	CCNGG	5 cut(s)	115, 711, 1146, 1224, 1230
MvaI	CCWGG	4 cut(s)	711, 1146, 1224, 1230
MvnI	CGCG	4 cut(s)	326, 382, 432, 817
MwoI	GCNNNNNNNGC	3 cut(s)	1064, 1164, 1173
NciI	CCSGG	1 cut(s)	115
NdeI	CATATG	1 cut(s)	654
NdeII	GATC	8 cut(s)	364, 418, 660, 706, 761, 818, 867, 967
NlaIII	CATG	4 cut(s)	592, 614, 1191, 1302
NlaIV	GGNNCC	4 cut(s)	258, 375, 486, 1244
NmuCI	GTSAC	2 cut(s)	472, 962
NruI	TCGCGA	1 cut(s)	432
PagI	TCATGA	1 cut(s)	588
PceI	AGGCCT	1 cut(s)	714
PdmI	GAANNNNTTC	1 cut(s)	1278
PfeI	GAWTC	2 cut(s)	206, 1208
PflMI	CCANNNNNTGG	2 cut(s)	137, 1229
PkrI	GCNGC	4 cut(s)	381, 1005, 1166, 1254
PpuMI	RGGWCCY	1 cut(s)	1226
PsiI	TTATAA	2 cut(s)	480, 560
Psp124BI	GAGCTC	1 cut(s)	542
Psp5II	RGGWCCY	1 cut(s)	1226
Psp6I	CCWGG	4 cut(s)	709, 1144, 1222, 1228
PspGI	CCWGG	4 cut(s)	709, 1144, 1222, 1228
PspN4I	GGNNCC	4 cut(s)	258, 375, 486, 1244
PspOMI	GGGCCC	1 cut(s)	484
PspPI	GGNCC	3 cut(s)	484, 485, 1226
PspPPI	RGGWCCY	1 cut(s)	1226
PstNI	CAGNNNCTG	1 cut(s)	1229
PsuI	RGATCY	1 cut(s)	364
RruI	TCGCGA	1 cut(s)	432
RsaI	GTAC	5 cut(s)	505, 719, 878, 1261, 1297
RsaNI	GTAC	5 cut(s)	504, 718, 877, 1260, 1296
SacI	GAGCTC	1 cut(s)	542
SaqAI	TTAA	3 cut(s)	320, 530, 1218
SatI	GCNGC	4 cut(s)	380, 1004, 1165, 1253
Sau3AI	GATC	8 cut(s)	364, 418, 660, 706, 761, 818, 867, 967
Sau96I	GGNCC	3 cut(s)	484, 485, 1226
ScrFI	CCNGG	5 cut(s)	115, 711, 1146, 1224, 1230
SduI	GDGCHC	4 cut(s)	259, 287, 488, 542
SfaNI	GCATC	2 cut(s)	581, 1015
SinI	GGWCC	1 cut(s)	1226
SmlI	CTYRAG	2 cut(s)	368, 827
SmoI	CTYRAG	2 cut(s)	368, 827
Sse9I	AATT	9 cut(s)	49, 145, 249, 395, 466, 702, 886, 924, 1104
SseBI	AGGCCT	1 cut(s)	714
SsiI	CCGC	5 cut(s)	326, 377, 380, 954, 1176
SspMI	CTAG	3 cut(s)	72, 261, 1121
SstI	GAGCTC	1 cut(s)	542
StuI	AGGCCT	1 cut(s)	714
StyD4I	CCNGG	5 cut(s)	113, 709, 1144, 1222, 1228
TaaI	ACNGT	1 cut(s)	922
TaqI	TCGA	3 cut(s)	156, 204, 1069
TasI	AATT	9 cut(s)	49, 145, 249, 395, 466, 702, 886, 924, 1104
TatI	WGTACW	4 cut(s)	503, 717, 876, 1295
TauI	GCSGC	1 cut(s)	382
TfiI	GAWTC	2 cut(s)	206, 1208
Tru1I	TTAA	3 cut(s)	320, 530, 1218
Tru9I	TTAA	3 cut(s)	320, 530, 1218
TscAI	CASTG	4 cut(s)	574, 670, 1098, 1218
TseFI	GTSAC	2 cut(s)	472, 962
TseI	GCWGC	3 cut(s)	1003, 1164, 1252
Tsp45I	GTSAC	2 cut(s)	472, 962
TspDTI	ATGAA	5 cut(s)	55, 76, 599, 905, 1040
TspGWI	ACGGA	1 cut(s)	1183
TspRI	CASTG	4 cut(s)	574, 670, 1098, 1218
Van91I	CCANNNNNTGG	2 cut(s)	137, 1229
VpaK11BI	GGWCC	1 cut(s)	1226
XapI	RAATTY	2 cut(s)	145, 886
XmnI	GAANNNNTTC	1 cut(s)	1278
XspI	CTAG	3 cut(s)	72, 261, 1121

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.