Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0006551

Physical Location & Seq

Genomic Coordinates Reverse (-)

74113355 .. 74115789

Gene Structure 2435 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000021320

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1302 bp

ATGAGGAAGTACGTGAATCACCATTCTGGAAGGGTTTTCGGTGATAGGGTTTGGGTCCTTCCATTCATTGCTAGCTTTGTCATATTCATTACCCTCTTTTTCTCAGTCATTTCTGGGCTGTTTACTTTGCCGCATGGTAAAGAACAGTTGCCAACCGACATTGGTTCCTTTTTGAAATCCGATGACTCAACTGGGTATTTTGTTGAGTCTGATTTGAAAAGATCATTGGAACAGAATAGACGTTTGAGAACAGGAGCACCTAGATTGGCGTATCTTATATCAGGTACAAAGGGGGATAGTCATAGAATGATGAGGACTTTGAGTGCTGTATATCATCCAAGAAATCAATATGTTCTGCATTTGGATCTCGAGGCTCCTCCTCGAGAAAGATTGGAACTTGCAAGTTTGGTTAAGGCTGATCCTACATTTAGTGAAGTGGAGAATGTGCGTGTTATGGCTCAATCCAATTTGGTGACGTATAAGGGCCCTACTATGATTGCTTGTACCCTGCAAGCGATTTCCATTTTGTTAAAGGAGAGCTCAGAGTGGGATTGGTTTATAAACCTCAGTGCGTCTGATTATCCTCTCATGACACAAGATGATTTGCTTCATTCCTTCTCTAATTTGTCTAGAGATATCAACTTCATCGAAAATATGCAGATTACTGGTTGGAAACTGAATCAAAGAGCAAAACCAATCATAATTGATCCAGGCCTGTATTTATCAAAAAAATCTGATCTTGCATGGACCACTCAGCGTCGTTCACTTCCTACATCATTCAAGTTGTTCACAGGTTCAGCATGGGTAATGCTAACACGGCCCTTCGTTGAGTACTGTATATGGGGATGGGATAACTTCCCTCGTACGATCCTTATGTACTATACGAATTTTGTTTCTTCTCCAGAAGGCTATTTTCATACTGTGATTTGTAACAATGAGGAATATCGCCACACTGCAATAAGCCATGATCTCCACTACATTGCTTGGGACAGCCCTCCGAAGCAGCATCCAGTCTCATTGTCAATGAAGGACTTTGATAAAATGGTAAAGAGCAATGCTCCTTTTGCACGAAAATTTGCAAAGAATGACCCAGTCTTGGATAAAATAGATAAAGAACTTCTAGGTCGAACAAGCCGGTTTTCTCCCGGAGCGTGGTGTATAGGTAGCTCAGAAGGCGGGGCTGACCCGTGTTCTATACGTGGTAATGATTCAATTTTTAGGCCAGGTCCTGGTGCTGAGAGGTTTCAAGTGTTGCTTCAGACATTGTTGTCTGAAGACTTTAGGAAAAAGCAATGTTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0006029 GO:0006807 GO:0007275 GO:0007399 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010033 GO:0010721 GO:0010975 GO:0010977 GO:0012505 GO:0016020 GO:0019538 GO:0022008 GO:0030154 GO:0030166 GO:0031090 GO:0031344 GO:0031345 GO:0031984 GO:0032101 GO:0032102 GO:0032501 GO:0032502 GO:0034097 GO:0034645 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045595 GO:0045596 GO:0045664 GO:0045665 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048679 GO:0048681 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050768 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051128 GO:0051129 GO:0051239 GO:0051241 GO:0051960 GO:0051961 GO:0060284 GO:0065007 GO:0070555 GO:0070570 GO:0070571 GO:0071704 GO:0080134 GO:0080135 GO:0098588 GO:0098791 GO:0120035 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1903034 GO:1903035 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Branch

Protein Analysis

434

Amino Acids

49.53

Weight (kDa)

8.86

Isoelectric Point (pI)

35.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Branch	PF02485	89 - 348	4.1e-78	Core-2/I-Branching enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006551)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03520 AT1G03520 AT4G03340 AT4G03340
fragaria_vesca	FvH4_6g44880
malus_domestica	MD09G1092300.v1.1 MD17G1082300.v1.1
prunus_persica	Prupe.3G232800_v2.0.a1 Prupe.3G232800_v2.0.a1 Prupe.3G232800_v2.0.a1 Prupe.3G232800_v2.0.a1 Prupe.3G232800_v2.0.a1 Prupe.3G232800_v2.0.a1
pyrus_communis	pycom17g08040 pycom17g27370
rosa_chinensis	RchiOBHm_Chr2g0162071 RchiOBHm_Chr3g0466861
rosa_laevigata	RLG00000021320
rosa_multiflora	Rmu_sc0005601.1_g000006
rosa_roxburghii	Rroxscaffold_2G00088330
rosa_rugosa	Rorug02G0494400
rosa_samantha	Rh2AG560600 Rh2BG573900 Rh2CG544200 Rh2DG583900
rosa_wichuraiana	Rw2G046430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	560
AasI	GACNNNNNNGTC	1 cut(s)	1267
AccB7I	CCANNNNNTGG	1 cut(s)	1229
AciI	CCGC	2 cut(s)	131, 1176
AclWI	GGATC	4 cut(s)	372, 413, 701, 862
AcsI	RAATTY	2 cut(s)	886, 1073
AcuI	CTGAAG	2 cut(s)	1241, 1293
AfaI	GTAC	6 cut(s)	11, 286, 505, 833, 865, 878
AfiI	CCNNNNNNNGG	3 cut(s)	1096, 1152, 1229
AgsI	TTSAA	5 cut(s)	175, 217, 781, 1212, 1247
AjnI	CCWGG	3 cut(s)	709, 1222, 1228
AjuI	GAANNNNNNNTTGG	2 cut(s)	209, 241
AluBI	AGCT	3 cut(s)	75, 540, 1167
AluI	AGCT	3 cut(s)	75, 540, 1167
Alw21I	GWGCWC	2 cut(s)	259, 542
Alw26I	GTCTC	1 cut(s)	1018
AlwI	GGATC	4 cut(s)	372, 413, 701, 862
AlwNI	CAGNNNCTG	1 cut(s)	1229
Ama87I	CYCGRG	2 cut(s)	368, 381
AoxI	GGCC	4 cut(s)	484, 712, 818, 1220
ApaI	GGGCCC	1 cut(s)	488
ApeKI	GCWGC	1 cut(s)	1003
ApoI	RAATTY	2 cut(s)	886, 1073
AspS9I	GGNCC	6 cut(s)	55, 484, 485, 747, 819, 1226
AsuC2I	CCSGG	1 cut(s)	1146
AsuHPI	GGTGA	3 cut(s)	11, 53, 484
AsuNHI	GCTAGC	1 cut(s)	71
AvaI	CYCGRG	2 cut(s)	368, 381
AvaII	GGWCC	3 cut(s)	55, 747, 1226
BaeGI	GKGCMC	1 cut(s)	488
BanII	GRGCYC	2 cut(s)	488, 542
BbsI	GAAGAC	1 cut(s)	1281
Bbv12I	GWGCWC	2 cut(s)	259, 542
BbvI	GCAGC	1 cut(s)	1015
BccI	CCATC	1 cut(s)	840
BceAI	ACGGC	1 cut(s)	833
BciT130I	CCWGG	3 cut(s)	711, 1224, 1230
BcnI	CCSGG	1 cut(s)	1146
BcoDI	GTCTC	1 cut(s)	1018
BfaI	CTAG	4 cut(s)	72, 261, 630, 1121
BisI	GCNGC	2 cut(s)	131, 1004
BlsI	GCNGC	2 cut(s)	132, 1005
BmcAI	AGTACT	1 cut(s)	833
Bme1390I	CCNGG	4 cut(s)	711, 1146, 1224, 1230
Bme18I	GGWCC	3 cut(s)	55, 747, 1226
BmeT110I	CYCGRG	2 cut(s)	368, 381
BmgT120I	GGNCC	6 cut(s)	55, 484, 485, 747, 819, 1226
BmiI	GGNNCC	4 cut(s)	56, 166, 375, 486
BmrFI	CCNGG	4 cut(s)	711, 1146, 1224, 1230
BmrI	ACTGGG	2 cut(s)	201, 1085
BmsI	GCATC	1 cut(s)	1015
BmtI	GCTAGC	1 cut(s)	75
BmuI	ACTGGG	2 cut(s)	201, 1085
BpiI	GAAGAC	1 cut(s)	1281
BplI	GAGNNNNNCTC	2 cut(s)	1042, 1074
BpmI	CTGGAG	1 cut(s)	885
BpuMI	CCSGG	1 cut(s)	1146
BsaAI	YACGTR	2 cut(s)	13, 1199
Bsc4I	CCNNNNNNNGG	3 cut(s)	1096, 1152, 1229
Bse118I	RCCGGY	1 cut(s)	1134
Bse1I	ACTGG	4 cut(s)	196, 670, 1010, 1091
Bse3DI	GCAATG	4 cut(s)	66, 978, 1060, 1298
BseBI	CCWGG	3 cut(s)	711, 1224, 1230
BseGI	GGATG	3 cut(s)	334, 851, 1006
BseLI	CCNNNNNNNGG	3 cut(s)	1096, 1152, 1229
BseMI	GCAATG	4 cut(s)	66, 978, 1060, 1298
BseMII	CTCAG	6 cut(s)	117, 555, 580, 767, 1182, 1227
BseNI	ACTGG	4 cut(s)	196, 670, 1010, 1091
BseRI	GAGGAG	2 cut(s)	366, 369
BseSI	GKGCMC	1 cut(s)	488
BseXI	GCAGC	1 cut(s)	1015
BshFI	GGCC	4 cut(s)	486, 714, 820, 1222
BsiHKAI	GWGCWC	2 cut(s)	259, 542
BsiHKCI	CYCGRG	2 cut(s)	368, 381
BsiSI	CCGG	2 cut(s)	1135, 1146
BsiWI	CGTACG	1 cut(s)	863
BslFI	GGGAC	1 cut(s)	1001
BslI	CCNNNNNNNGG	3 cut(s)	1096, 1152, 1229
BsmAI	GTCTC	1 cut(s)	1018
BsmFI	GGGAC	1 cut(s)	1001
BsnI	GGCC	4 cut(s)	486, 714, 820, 1222
BsoBI	CYCGRG	2 cut(s)	368, 381
Bsp120I	GGGCCC	1 cut(s)	484
Bsp1286I	GDGCHC	3 cut(s)	259, 488, 542
Bsp143I	GATC	7 cut(s)	221, 364, 418, 706, 736, 867, 967
BspACI	CCGC	2 cut(s)	131, 1176
BspANI	GGCC	4 cut(s)	486, 714, 820, 1222
BspCNI	CTCAG	6 cut(s)	116, 554, 579, 766, 1181, 1228
BspHI	TCATGA	2 cut(s)	588, 1298
BspLI	GGNNCC	4 cut(s)	56, 166, 375, 486
BspOI	GCTAGC	1 cut(s)	75
BspPI	GGATC	4 cut(s)	372, 413, 701, 862
BsrDI	GCAATG	4 cut(s)	66, 978, 1060, 1298
BsrFI	RCCGGY	1 cut(s)	1134
BsrI	ACTGG	4 cut(s)	196, 670, 1010, 1091
BssAI	RCCGGY	1 cut(s)	1134
BssMI	GATC	7 cut(s)	221, 364, 418, 706, 736, 867, 967
Bst2UI	CCWGG	3 cut(s)	711, 1224, 1230
Bst4CI	ACNGT	3 cut(s)	147, 836, 922
BstBAI	YACGTR	2 cut(s)	13, 1199
BstC8I	GCNNGC	2 cut(s)	73, 513
BstDEI	CTNAG	6 cut(s)	103, 541, 566, 753, 1168, 1236
BstF5I	GGATG	3 cut(s)	334, 851, 1006
BstKTI	GATC	7 cut(s)	224, 367, 421, 709, 739, 870, 970
BstMAI	GTCTC	1 cut(s)	1018
BstMBI	GATC	7 cut(s)	221, 364, 418, 706, 736, 867, 967
BstMWI	GCNNNNNNNGC	3 cut(s)	817, 1064, 1173
BstNI	CCWGG	3 cut(s)	711, 1224, 1230
BstSCI	CCNGG	4 cut(s)	709, 1144, 1222, 1228
BstSLI	GKGCMC	1 cut(s)	488
BstV1I	GCAGC	1 cut(s)	1015
BstV2I	GAAGAC	1 cut(s)	1281
BstX2I	RGATCY	1 cut(s)	364
BstYI	RGATCY	1 cut(s)	364
BsuRI	GGCC	4 cut(s)	486, 714, 820, 1222
BtsCI	GGATG	3 cut(s)	334, 851, 1006
BtsI	GCAGTG	1 cut(s)	951
BtsIMutI	CAGTG	2 cut(s)	574, 951
Cac8I	GCNNGC	2 cut(s)	73, 513
CaiI	CAGNNNCTG	1 cut(s)	1229
CciI	TCATGA	2 cut(s)	588, 1298
Cfr10I	RCCGGY	1 cut(s)	1134
Cfr13I	GGNCC	6 cut(s)	55, 484, 485, 747, 819, 1226
CseI	GACGC	2 cut(s)	561, 746
Csp6I	GTAC	6 cut(s)	10, 285, 504, 832, 864, 877
CviAII	CATG	6 cut(s)	134, 589, 744, 801, 965, 1299
CviQI	GTAC	6 cut(s)	10, 285, 504, 832, 864, 877
DdeI	CTNAG	6 cut(s)	103, 541, 566, 753, 1168, 1236
DpnI	GATC	7 cut(s)	223, 366, 420, 708, 738, 869, 969
DpnII	GATC	7 cut(s)	221, 364, 418, 706, 736, 867, 967
DrdI	GACNNNNNNGTC	1 cut(s)	1267
DseDI	GACNNNNNNGTC	1 cut(s)	1267
Ecl136II	GAGCTC	1 cut(s)	540
Eco147I	AGGCCT	1 cut(s)	714
Eco24I	GRGCYC	2 cut(s)	488, 542
Eco32I	GATATC	1 cut(s)	637
Eco47I	GGWCC	3 cut(s)	55, 747, 1226
Eco53kI	GAGCTC	1 cut(s)	540
Eco57I	CTGAAG	2 cut(s)	1241, 1293
Eco88I	CYCGRG	2 cut(s)	368, 381
EcoICRI	GAGCTC	1 cut(s)	540
EcoO109I	RGGNCCY	4 cut(s)	55, 484, 485, 1226
EcoRII	CCWGG	3 cut(s)	709, 1222, 1228
EcoRV	GATATC	1 cut(s)	637
EcoT38I	GRGCYC	2 cut(s)	488, 542
FaeI	CATG	6 cut(s)	137, 592, 747, 804, 968, 1302
FalI	AAGNNNNNCTT	2 cut(s)	1239, 1271
FaqI	GGGAC	1 cut(s)	1001
FatI	CATG	6 cut(s)	133, 588, 743, 800, 964, 1298
FauI	CCCGC	1 cut(s)	1169
Fnu4HI	GCNGC	2 cut(s)	131, 1004
FokI	GGATG	3 cut(s)	321, 858, 993
FriOI	GRGCYC	2 cut(s)	488, 542
Fsp4HI	GCNGC	2 cut(s)	131, 1004
FspBI	CTAG	4 cut(s)	72, 261, 630, 1121
GluI	GCNGC	2 cut(s)	131, 1004
GsuI	CTGGAG	1 cut(s)	885
HaeIII	GGCC	4 cut(s)	486, 714, 820, 1222
HapII	CCGG	2 cut(s)	1135, 1146
HgaI	GACGC	2 cut(s)	561, 746
Hin1II	CATG	6 cut(s)	137, 592, 747, 804, 968, 1302
HinfI	GANTC	5 cut(s)	16, 185, 206, 679, 1208
HpaII	CCGG	2 cut(s)	1135, 1146
HphI	GGTGA	3 cut(s)	11, 53, 484
Hpy166II	GTNNAC	3 cut(s)	123, 764, 789
Hpy188I	TCNGA	9 cut(s)	181, 211, 544, 577, 736, 999, 1171, 1260, 1273
Hpy188III	TCNNGA	7 cut(s)	27, 368, 383, 589, 630, 902, 1299
Hpy8I	GTNNAC	3 cut(s)	123, 764, 789
Hpy99I	CGWCG	1 cut(s)	762
HpyAV	CCTTC	7 cut(s)	24, 68, 625, 832, 899, 1021, 1166
HpyCH4III	ACNGT	3 cut(s)	147, 836, 922
HpyCH4IV	ACGT	4 cut(s)	12, 241, 476, 1198
HpyCH4V	TGCA	8 cut(s)	358, 401, 511, 658, 743, 956, 1067, 1079
HpyF10VI	GCNNNNNNNGC	3 cut(s)	817, 1064, 1173
HpyF3I	CTNAG	6 cut(s)	103, 541, 566, 753, 1168, 1236
HpySE526I	ACGT	4 cut(s)	12, 241, 476, 1198
Hsp92II	CATG	6 cut(s)	137, 592, 747, 804, 968, 1302
Kzo9I	GATC	7 cut(s)	221, 364, 418, 706, 736, 867, 967
LmnI	GCTCC	4 cut(s)	254, 379, 1063, 1148
Lsp1109I	GCAGC	1 cut(s)	1015
LweI	GCATC	1 cut(s)	1015
MaeI	CTAG	4 cut(s)	72, 261, 630, 1121
MaeII	ACGT	4 cut(s)	12, 241, 476, 1198
MaeIII	GTNAC	2 cut(s)	472, 929
MalI	GATC	7 cut(s)	223, 366, 420, 708, 738, 869, 969
MboI	GATC	7 cut(s)	221, 364, 418, 706, 736, 867, 967
MboII	GAAGA	2 cut(s)	888, 1286
MflI	RGATCY	1 cut(s)	364
MhlI	GDGCHC	3 cut(s)	259, 488, 542
MluCI	AATT	6 cut(s)	466, 622, 702, 886, 1073, 1212
MlyI	GAGTC	2 cut(s)	179, 215
MmeI	TCCRAC	1 cut(s)	650
MseI	TTAA	2 cut(s)	411, 530
MspI	CCGG	2 cut(s)	1135, 1146
MspR9I	CCNGG	4 cut(s)	711, 1146, 1224, 1230
MvaI	CCWGG	3 cut(s)	711, 1224, 1230
MwoI	GCNNNNNNNGC	3 cut(s)	817, 1064, 1173
NciI	CCSGG	1 cut(s)	1146
NdeII	GATC	7 cut(s)	221, 364, 418, 706, 736, 867, 967
NheI	GCTAGC	1 cut(s)	71
NlaIII	CATG	6 cut(s)	137, 592, 747, 804, 968, 1302
NlaIV	GGNNCC	4 cut(s)	56, 166, 375, 486
NmuCI	GTSAC	1 cut(s)	472
PaeR7I	CTCGAG	2 cut(s)	368, 381
PagI	TCATGA	2 cut(s)	588, 1298
PceI	AGGCCT	1 cut(s)	714
PfeI	GAWTC	3 cut(s)	16, 679, 1208
Pfl23II	CGTACG	1 cut(s)	863
PflFI	GACNNNGTC	1 cut(s)	1091
PflMI	CCANNNNNTGG	1 cut(s)	1229
PfoI	TCCNGGA	1 cut(s)	1144
PkrI	GCNGC	2 cut(s)	132, 1005
PleI	GAGTC	2 cut(s)	179, 214
PpsI	GAGTC	2 cut(s)	179, 214
Ppu21I	YACGTR	2 cut(s)	13, 1199
PpuMI	RGGWCCY	2 cut(s)	55, 1226
PsiI	TTATAA	1 cut(s)	560
Psp124BI	GAGCTC	1 cut(s)	542
Psp5II	RGGWCCY	2 cut(s)	55, 1226
Psp6I	CCWGG	3 cut(s)	709, 1222, 1228
PspGI	CCWGG	3 cut(s)	709, 1222, 1228
PspLI	CGTACG	1 cut(s)	863
PspN4I	GGNNCC	4 cut(s)	56, 166, 375, 486
PspOMI	GGGCCC	1 cut(s)	484
PspPI	GGNCC	6 cut(s)	55, 484, 485, 747, 819, 1226
PspPPI	RGGWCCY	2 cut(s)	55, 1226
PstNI	CAGNNNCTG	1 cut(s)	1229
PsuI	RGATCY	1 cut(s)	364
PsyI	GACNNNGTC	1 cut(s)	1091
RsaI	GTAC	6 cut(s)	11, 286, 505, 833, 865, 878
RsaNI	GTAC	6 cut(s)	10, 285, 504, 832, 864, 877
SacI	GAGCTC	1 cut(s)	542
SaqAI	TTAA	2 cut(s)	411, 530
SatI	GCNGC	2 cut(s)	131, 1004
Sau3AI	GATC	7 cut(s)	221, 364, 418, 706, 736, 867, 967
Sau96I	GGNCC	6 cut(s)	55, 484, 485, 747, 819, 1226
ScaI	AGTACT	1 cut(s)	833
SchI	GAGTC	2 cut(s)	179, 215
ScrFI	CCNGG	4 cut(s)	711, 1146, 1224, 1230
SduI	GDGCHC	3 cut(s)	259, 488, 542
SfaNI	GCATC	1 cut(s)	1015
Sfr274I	CTCGAG	2 cut(s)	368, 381
SinI	GGWCC	3 cut(s)	55, 747, 1226
SlaI	CTCGAG	2 cut(s)	368, 381
SmlI	CTYRAG	2 cut(s)	368, 381
SmoI	CTYRAG	2 cut(s)	368, 381
Sse9I	AATT	6 cut(s)	466, 622, 702, 886, 1073, 1212
SseBI	AGGCCT	1 cut(s)	714
SsiI	CCGC	2 cut(s)	131, 1176
SspMI	CTAG	4 cut(s)	72, 261, 630, 1121
SstI	GAGCTC	1 cut(s)	542
StuI	AGGCCT	1 cut(s)	714
StyD4I	CCNGG	4 cut(s)	709, 1144, 1222, 1228
TaaI	ACNGT	3 cut(s)	147, 836, 922
TaiI	ACGT	4 cut(s)	15, 244, 479, 1201
TaqI	TCGA	4 cut(s)	369, 382, 648, 1126
TasI	AATT	6 cut(s)	466, 622, 702, 886, 1073, 1212
TatI	WGTACW	2 cut(s)	831, 876
TauI	GCSGC	1 cut(s)	133
TfiI	GAWTC	3 cut(s)	16, 679, 1208
Tru1I	TTAA	2 cut(s)	411, 530
Tru9I	TTAA	2 cut(s)	411, 530
TscAI	CASTG	2 cut(s)	574, 958
TseFI	GTSAC	1 cut(s)	472
TseI	GCWGC	1 cut(s)	1003
Tsp45I	GTSAC	1 cut(s)	472
TspDTI	ATGAA	7 cut(s)	55, 76, 599, 634, 905, 1040, 1287
TspRI	CASTG	2 cut(s)	574, 958
Tth111I	GACNNNGTC	1 cut(s)	1091
Van91I	CCANNNNNTGG	1 cut(s)	1229
VpaK11BI	GGWCC	3 cut(s)	55, 747, 1226
XapI	RAATTY	2 cut(s)	886, 1073
XbaI	TCTAGA	1 cut(s)	629
XhoI	CTCGAG	2 cut(s)	368, 381
XspI	CTAG	4 cut(s)	72, 261, 630, 1121
ZrmI	AGTACT	1 cut(s)	833

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.