RchiOBHm_Chr3g0466861

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0006551
Physical Location & Seq
Reverse (-)
13200332 .. 13202835
2504 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43288

Sequence Viewer

Length: 1266 bp
ATGAAACGAACCAGCCACAATCAGCCTCAATGGCTCGTCATTCTAACCGCGACCTCCGCCCTCCTCCTCCTCGCTCTCCTCGCCCTCACCACCACCCACGCCAAGTACTCCTCCTCCTCCTCCTCCGGCCCCGACTTCACCTACGACCACCGCCGATTCCCTAATCCCGACCAGTATTTCGTCGACAGCGACGACGACGACGAGAACTACGGCCTAGGGCTCCCGAGATTCGCTTACCTGATTACCGGTTCTGAAGGTGACGGCCCTCAGCTGAAGCGGCTGCTTCAGGCGGCGTACCACCCGAGAAATTACTACTTGCTCCACCTCGATCTGGAAGCTTCCGACGCCGAGAGGCTCGAGCTCGCCAAGTACGTCAAGTCCGAGAGTGTGATGAACGCGTTTCGGAATGCGATGGTGGTTGGAACCGCGGATTTGGTGACCGCGAAAGGTCCGACGGTGTTGGCGGCGACGCTGCACGCGGTTGCGATTTTGCTCAAGCGGGCCGAGGATTGGGATTGGTTTATTAATCTCAGTGCTTCGGATTATCCGCTCATGTCCCAAGATGATTTGCTGCATATATTTTCATACTTGCCAAGGGACCTCAACTTTCTGGAGCACACTAGCAATATTGGGTGGAAAGAAAATCAAAGAGCGAGGCCTATTATAATTGATCCGGGTTTGTACCACACTAAGAAATCCGGTGTGTTTTGGGCTAAAGAGAGAAGGTCACTGCCAGCTTCCTTCAAGTTATTCATGGGGTCTACAGGGGTGGTGCTGACAAGATCATTCCTTGAATTCTGTGTTTTGGGATGGGACAATCTTCCCCGCACTCTTCTCATGTATTATACCAATTTCCTGTCATCCACAGAGGGCTACTTCCACACTGTTATCTGCAACCATAAGGATTACCAGAACACGACTGTGAATCATGACTTGCATTACATAAGGTGGGATAGTCCTCCAAAGCTAAATCCAGTTCATCTAACACTGGAACATTACAAAGACATCATCCAAGCGGGAGCTCCCTTCGCCAGTCCATTTGTGAGGGATGATCCGGTTCTTGACAAAATTGATAAGGAGCTCTTGAGGAGACCAAGCGGGCATGTTACACCCGGTGGTTGGTGTTTAGGAAGTACAGGTAAAGATAGATGTTCAGTTTATGGGAATCCAGTGGCTGTAAAACCTACTGTTGTTTCAAAGAGACTAGAGAAACTGATTGTGAAACTTCTCGATTCTGAAAATTTCAGGTCCAAGCAGTGTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008194 GO:0015020 GO:0016740 GO:0016757 GO:0016758
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

421

Amino Acids

47.75

Weight (kDa)

7.71

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 77 - 336 4.8e-71 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 665
AccBSI CCGCTC 1 cut(s) 550
AccI GTMKAC 2 cut(s) 183, 761
AccII CGCG 5 cut(s) 50, 398, 428, 443, 479
AclWI GGATC 2 cut(s) 665, 1046
AcsI RAATTY 2 cut(s) 794, 1240
AcuI CTGAAG 3 cut(s) 269, 273, 293
AcyI GRCGYC 1 cut(s) 345
AdeI CACNNNGTG 1 cut(s) 1115
AfaI GTAC 5 cut(s) 107, 296, 371, 683, 1135
AfiI CCNNNNNNNGG 3 cut(s) 331, 510, 1119
AflIII ACRYGT 1 cut(s) 396
AgeI ACCGGT 1 cut(s) 245
AgsI TTSAA 3 cut(s) 745, 794, 1197
AleI CACNNNNGTG 1 cut(s) 920
AluBI AGCT 7 cut(s) 271, 338, 361, 737, 967, 1022, 1081
AluI AGCT 7 cut(s) 271, 338, 361, 737, 967, 1022, 1081
Alw21I GWGCWC 4 cut(s) 363, 618, 1024, 1083
Alw26I GTCTC 2 cut(s) 1084, 1195
AlwI GGATC 2 cut(s) 665, 1046
AlwNI CAGNNNCTG 1 cut(s) 1175
Ama87I CYCGRG 3 cut(s) 223, 301, 356
AoxI GGCC 5 cut(s) 127, 211, 262, 501, 656
ApeKI GCWGC 3 cut(s) 280, 472, 571
ApoI RAATTY 2 cut(s) 794, 1240
ArsI GACNNNNNNTTYG 2 cut(s) 161, 193
AseI ATTAAT 1 cut(s) 525
AsiGI ACCGGT 1 cut(s) 245
AspA2I CCTAGG 1 cut(s) 214
AspS9I GGNCC 6 cut(s) 128, 263, 449, 501, 598, 1248
AsuC2I CCSGG 2 cut(s) 675, 1113
AsuHPI GGTGA 4 cut(s) 79, 130, 269, 448
AvaI CYCGRG 3 cut(s) 223, 301, 356
AvaII GGWCC 3 cut(s) 449, 598, 1248
AvrII CCTAGG 1 cut(s) 214
BanII GRGCYC 4 cut(s) 222, 363, 1024, 1083
Bbv12I GWGCWC 4 cut(s) 363, 618, 1024, 1083
BbvCI CCTCAGC 1 cut(s) 267
BbvI GCAGC 3 cut(s) 267, 459, 558
BccI CCATC 2 cut(s) 406, 804
BceAI ACGGC 2 cut(s) 226, 277
BcnI CCSGG 2 cut(s) 675, 1113
BcoDI GTCTC 2 cut(s) 1084, 1195
BfaI CTAG 3 cut(s) 215, 621, 1205
BfmI CTRYAG 1 cut(s) 762
BglI GCCNNNNNGGC 1 cut(s) 31
BisI GCNGC 6 cut(s) 278, 281, 291, 465, 473, 572
BlnI CCTAGG 1 cut(s) 214
BlsI GCNGC 6 cut(s) 279, 282, 292, 466, 474, 573
BmcAI AGTACT 1 cut(s) 107
Bme1390I CCNGG 2 cut(s) 675, 1113
Bme18I GGWCC 3 cut(s) 449, 598, 1248
BmeT110I CYCGRG 3 cut(s) 223, 301, 356
BmgT120I GGNCC 6 cut(s) 128, 263, 449, 501, 598, 1248
BmiI GGNNCC 4 cut(s) 130, 221, 424, 599
BmrFI CCNGG 2 cut(s) 675, 1113
BpmI CTGGAG 1 cut(s) 632
Bpu10I CCTNAGC 1 cut(s) 267
BpuEI CTTGAG 2 cut(s) 479, 1105
BpuMI CCSGG 2 cut(s) 675, 1113
BsaHI GRCGYC 1 cut(s) 345
BsaI GGTCTC 1 cut(s) 1084
BsaJI CCNNGG 4 cut(s) 214, 426, 504, 593
BsaWI WCCGGW 3 cut(s) 245, 698, 1054
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 3 cut(s) 331, 510, 1119
Bse118I RCCGGY 1 cut(s) 245
Bse1I ACTGG 5 cut(s) 172, 974, 993, 1032, 1169
BseDI CCNNGG 4 cut(s) 214, 426, 504, 593
BseGI GGATG 4 cut(s) 815, 860, 1008, 1054
BseLI CCNNNNNNNGG 3 cut(s) 331, 510, 1119
BseMII CTCAG 2 cut(s) 281, 544
BseNI ACTGG 5 cut(s) 172, 974, 993, 1032, 1169
BseXI GCAGC 3 cut(s) 267, 459, 558
BsgI GTGCAG 1 cut(s) 458
Bsh1236I CGCG 5 cut(s) 50, 398, 428, 443, 479
BshFI GGCC 5 cut(s) 129, 213, 264, 503, 658
BshTI ACCGGT 1 cut(s) 245
BsiHKAI GWGCWC 4 cut(s) 363, 618, 1024, 1083
BsiHKCI CYCGRG 3 cut(s) 223, 301, 356
BsiSI CCGG 6 cut(s) 126, 246, 674, 699, 1055, 1113
BslFI GGGAC 3 cut(s) 541, 611, 827
BslI CCNNNNNNNGG 3 cut(s) 331, 510, 1119
BsmAI GTCTC 2 cut(s) 1084, 1195
BsmFI GGGAC 3 cut(s) 541, 611, 827
BsmI GAATGC 1 cut(s) 412
BsnI GGCC 5 cut(s) 129, 213, 264, 503, 658
Bso31I GGTCTC 1 cut(s) 1084
BsoBI CYCGRG 3 cut(s) 223, 301, 356
Bsp1286I GDGCHC 5 cut(s) 222, 363, 618, 1024, 1083
Bsp143I GATC 4 cut(s) 328, 670, 782, 1051
BspANI GGCC 5 cut(s) 129, 213, 264, 503, 658
BspCNI CTCAG 2 cut(s) 280, 543
BspFNI CGCG 5 cut(s) 50, 398, 428, 443, 479
BspHI TCATGA 1 cut(s) 928
BspLI GGNNCC 4 cut(s) 130, 221, 424, 599
BspPI GGATC 2 cut(s) 665, 1046
BspTNI GGTCTC 1 cut(s) 1084
BsrBI CCGCTC 1 cut(s) 550
BsrFI RCCGGY 1 cut(s) 245
BsrI ACTGG 5 cut(s) 172, 974, 993, 1032, 1169
BssAI RCCGGY 1 cut(s) 245
BssECI CCNNGG 4 cut(s) 214, 426, 504, 593
BssMI GATC 4 cut(s) 328, 670, 782, 1051
BssNI GRCGYC 1 cut(s) 345
BssT1I CCWWGG 2 cut(s) 214, 593
Bst4CI ACNGT 4 cut(s) 457, 886, 922, 1189
Bst6I CTCTTC 1 cut(s) 837
BstACI GRCGYC 1 cut(s) 345
BstC8I GCNNGC 5 cut(s) 363, 477, 501, 735, 1100
BstDEI CTNAG 3 cut(s) 267, 530, 690
BstDSI CCRYGG 1 cut(s) 426
BstEII GGTNACC 1 cut(s) 436
BstF5I GGATG 4 cut(s) 815, 860, 1008, 1054
BstFNI CGCG 5 cut(s) 50, 398, 428, 443, 479
BstKTI GATC 4 cut(s) 331, 673, 785, 1054
BstMAI GTCTC 2 cut(s) 1084, 1195
BstMBI GATC 4 cut(s) 328, 670, 782, 1051
BstMWI GCNNNNNNNGC 6 cut(s) 31, 56, 80, 277, 344, 1028
BstNSI RCATGY 1 cut(s) 1106
BstPI GGTNACC 1 cut(s) 436
BstSCI CCNGG 2 cut(s) 673, 1111
BstSFI CTRYAG 1 cut(s) 762
BstUI CGCG 5 cut(s) 50, 398, 428, 443, 479
BstV1I GCAGC 3 cut(s) 267, 459, 558
BsuRI GGCC 5 cut(s) 129, 213, 264, 503, 658
BtgI CCRYGG 1 cut(s) 426
BtgZI GCGATG 1 cut(s) 425
BtsCI GGATG 4 cut(s) 815, 860, 1008, 1054
BtsI GCAGTG 2 cut(s) 728, 1262
BtsIMutI CAGTG 6 cut(s) 538, 728, 882, 986, 1176, 1262
Cac8I GCNNGC 5 cut(s) 363, 477, 501, 735, 1100
CaiI CAGNNNCTG 1 cut(s) 1175
CciI TCATGA 1 cut(s) 928
Cfr10I RCCGGY 1 cut(s) 245
Cfr13I GGNCC 6 cut(s) 128, 263, 449, 501, 598, 1248
Cfr42I CCGCGG 1 cut(s) 429
CseI GACGC 2 cut(s) 353, 478
Csp6I GTAC 5 cut(s) 106, 295, 370, 682, 1134
CspAI ACCGGT 1 cut(s) 245
CviAII CATG 5 cut(s) 553, 754, 838, 929, 1103
CviQI GTAC 5 cut(s) 106, 295, 370, 682, 1134
DdeI CTNAG 3 cut(s) 267, 530, 690
DpnI GATC 4 cut(s) 330, 672, 784, 1053
DpnII GATC 4 cut(s) 328, 670, 782, 1051
DraIII CACNNNGTG 1 cut(s) 1115
Eam1104I CTCTTC 1 cut(s) 837
EarI CTCTTC 1 cut(s) 837
EciI GGCGGA 1 cut(s) 46
Ecl136II GAGCTC 3 cut(s) 361, 1022, 1081
Eco130I CCWWGG 2 cut(s) 214, 593
Eco147I AGGCCT 1 cut(s) 658
Eco24I GRGCYC 4 cut(s) 222, 363, 1024, 1083
Eco31I GGTCTC 1 cut(s) 1084
Eco47I GGWCC 3 cut(s) 449, 598, 1248
Eco53kI GAGCTC 3 cut(s) 361, 1022, 1081
Eco57I CTGAAG 3 cut(s) 269, 273, 293
Eco88I CYCGRG 3 cut(s) 223, 301, 356
Eco91I GGTNACC 1 cut(s) 436
EcoICRI GAGCTC 3 cut(s) 361, 1022, 1081
EcoO109I RGGNCCY 1 cut(s) 598
EcoO65I GGTNACC 1 cut(s) 436
EcoRI GAATTC 1 cut(s) 794
EcoT14I CCWWGG 2 cut(s) 214, 593
EcoT38I GRGCYC 4 cut(s) 222, 363, 1024, 1083
ErhI CCWWGG 2 cut(s) 214, 593
FaeI CATG 5 cut(s) 556, 757, 841, 932, 1106
FalI AAGNNNNNCTT 2 cut(s) 1067, 1099
FaqI GGGAC 3 cut(s) 541, 611, 827
FatI CATG 5 cut(s) 552, 753, 837, 928, 1102
FauI CCCGC 4 cut(s) 492, 833, 1009, 1091
FblI GTMKAC 2 cut(s) 183, 761
Fnu4HI GCNGC 6 cut(s) 278, 281, 291, 465, 473, 572
FokI GGATG 4 cut(s) 822, 847, 995, 1061
FriOI GRGCYC 4 cut(s) 222, 363, 1024, 1083
Fsp4HI GCNGC 6 cut(s) 278, 281, 291, 465, 473, 572
FspBI CTAG 3 cut(s) 215, 621, 1205
GluI GCNGC 6 cut(s) 278, 281, 291, 465, 473, 572
GsuI CTGGAG 1 cut(s) 632
HaeIII GGCC 5 cut(s) 129, 213, 264, 503, 658
HapII CCGG 6 cut(s) 126, 246, 674, 699, 1055, 1113
HgaI GACGC 2 cut(s) 353, 478
Hin1I GRCGYC 1 cut(s) 345
Hin1II CATG 5 cut(s) 556, 757, 841, 932, 1106
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HindIII AAGCTT 1 cut(s) 336
HinfI GANTC 5 cut(s) 156, 228, 925, 1165, 1232
HpaII CCGG 6 cut(s) 126, 246, 674, 699, 1055, 1113
HphI GGTGA 4 cut(s) 79, 130, 269, 448
Hpy166II GTNNAC 2 cut(s) 184, 762
Hpy188I TCNGA 7 cut(s) 253, 343, 382, 405, 453, 541, 1237
Hpy188III TCNNGA 8 cut(s) 167, 223, 332, 611, 929, 1061, 1084, 1229
Hpy8I GTNNAC 2 cut(s) 184, 762
Hpy99I CGWCG 8 cut(s) 185, 194, 197, 200, 203, 347, 457, 472
HpyAV CCTTC 4 cut(s) 248, 717, 751, 1036
HpyCH4III ACNGT 4 cut(s) 457, 886, 922, 1189
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 4 cut(s) 475, 574, 894, 937
HpyF10VI GCNNNNNNNGC 6 cut(s) 31, 56, 80, 277, 344, 1028
HpyF3I CTNAG 3 cut(s) 267, 530, 690
HpySE526I ACGT 1 cut(s) 372
Hsp92I GRCGYC 1 cut(s) 345
Hsp92II CATG 5 cut(s) 556, 757, 841, 932, 1106
KspI CCGCGG 1 cut(s) 429
Kzo9I GATC 4 cut(s) 328, 670, 782, 1051
LmnI GCTCC 6 cut(s) 225, 324, 613, 1019, 1027, 1078
Lsp1109I GCAGC 3 cut(s) 267, 459, 558
MaeI CTAG 3 cut(s) 215, 621, 1205
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 4 cut(s) 257, 436, 726, 1105
MalI GATC 4 cut(s) 330, 672, 784, 1053
MbiI CCGCTC 1 cut(s) 550
MboI GATC 4 cut(s) 328, 670, 782, 1051
MboII GAAGA 2 cut(s) 812, 824
MhlI GDGCHC 5 cut(s) 222, 363, 618, 1024, 1083
MluCI AATT 6 cut(s) 307, 666, 794, 850, 1068, 1240
MluI ACGCGT 1 cut(s) 396
MmeI TCCRAC 3 cut(s) 366, 400, 476
MseI TTAA 1 cut(s) 525
MslI CAYNNNNRTG 1 cut(s) 920
MspA1I CMGCKG 2 cut(s) 271, 428
MspI CCGG 6 cut(s) 126, 246, 674, 699, 1055, 1113
MspR9I CCNGG 2 cut(s) 675, 1113
Mva1269I GAATGC 1 cut(s) 412
MvnI CGCG 5 cut(s) 50, 398, 428, 443, 479
MwoI GCNNNNNNNGC 6 cut(s) 31, 56, 80, 277, 344, 1028
NciI CCSGG 2 cut(s) 675, 1113
NdeII GATC 4 cut(s) 328, 670, 782, 1051
NlaIII CATG 5 cut(s) 556, 757, 841, 932, 1106
NlaIV GGNNCC 4 cut(s) 130, 221, 424, 599
NmeAIII GCCGAG 2 cut(s) 373, 529
NmuCI GTSAC 3 cut(s) 257, 436, 726
NspI RCATGY 1 cut(s) 1106
OliI CACNNNNGTG 1 cut(s) 920
PaeR7I CTCGAG 1 cut(s) 356
PagI TCATGA 1 cut(s) 928
PceI AGGCCT 1 cut(s) 658
PcsI WCGNNNNNNNCGW 5 cut(s) 186, 189, 198, 369, 378
PctI GAATGC 1 cut(s) 412
PfeI GAWTC 5 cut(s) 156, 228, 925, 1165, 1232
PinAI ACCGGT 1 cut(s) 245
PkrI GCNGC 6 cut(s) 279, 282, 292, 466, 474, 573
PpuMI RGGWCCY 1 cut(s) 598
PshBI ATTAAT 1 cut(s) 525
PsiI TTATAA 1 cut(s) 665
Psp124BI GAGCTC 3 cut(s) 363, 1024, 1083
Psp5II RGGWCCY 1 cut(s) 598
PspEI GGTNACC 1 cut(s) 436
PspN4I GGNNCC 4 cut(s) 130, 221, 424, 599
PspPI GGNCC 6 cut(s) 128, 263, 449, 501, 598, 1248
PspPPI RGGWCCY 1 cut(s) 598
PspXI VCTCGAGB 1 cut(s) 356
PstNI CAGNNNCTG 1 cut(s) 1175
PvuII CAGCTG 1 cut(s) 271
RsaI GTAC 5 cut(s) 107, 296, 371, 683, 1135
RsaNI GTAC 5 cut(s) 106, 295, 370, 682, 1134
RseI CAYNNNNRTG 1 cut(s) 920
SacI GAGCTC 3 cut(s) 363, 1024, 1083
SacII CCGCGG 1 cut(s) 429
SalI GTCGAC 1 cut(s) 182
SaqAI TTAA 1 cut(s) 525
SatI GCNGC 6 cut(s) 278, 281, 291, 465, 473, 572
Sau3AI GATC 4 cut(s) 328, 670, 782, 1051
Sau96I GGNCC 6 cut(s) 128, 263, 449, 501, 598, 1248
ScaI AGTACT 1 cut(s) 107
ScrFI CCNGG 2 cut(s) 675, 1113
SduI GDGCHC 5 cut(s) 222, 363, 618, 1024, 1083
SfcI CTRYAG 1 cut(s) 762
Sfr274I CTCGAG 1 cut(s) 356
Sfr303I CCGCGG 1 cut(s) 429
SgrBI CCGCGG 1 cut(s) 429
SinI GGWCC 3 cut(s) 449, 598, 1248
SlaI CTCGAG 1 cut(s) 356
SmiMI CAYNNNNRTG 1 cut(s) 920
SmlI CTYRAG 3 cut(s) 356, 494, 1084
SmoI CTYRAG 3 cut(s) 356, 494, 1084
Sse9I AATT 6 cut(s) 307, 666, 794, 850, 1068, 1240
SseBI AGGCCT 1 cut(s) 658
SspI AATATT 1 cut(s) 628
SspMI CTAG 3 cut(s) 215, 621, 1205
SstI GAGCTC 3 cut(s) 363, 1024, 1083
StuI AGGCCT 1 cut(s) 658
StyD4I CCNGG 2 cut(s) 673, 1111
StyI CCWWGG 2 cut(s) 214, 593
TaaI ACNGT 4 cut(s) 457, 886, 922, 1189
TaiI ACGT 1 cut(s) 375
TaqI TCGA 4 cut(s) 183, 327, 357, 1230
TasI AATT 6 cut(s) 307, 666, 794, 850, 1068, 1240
TatI WGTACW 2 cut(s) 105, 1133
TauI GCSGC 3 cut(s) 280, 293, 467
TfiI GAWTC 5 cut(s) 156, 228, 925, 1165, 1232
Tru1I TTAA 1 cut(s) 525
Tru9I TTAA 1 cut(s) 525
TscAI CASTG 6 cut(s) 538, 735, 889, 993, 1176, 1262
TseFI GTSAC 3 cut(s) 257, 436, 726
TseI GCWGC 3 cut(s) 280, 472, 571
Tsp45I GTSAC 3 cut(s) 257, 436, 726
TspDTI ATGAA 5 cut(s) 17, 407, 573, 742, 968
TspRI CASTG 6 cut(s) 538, 735, 889, 993, 1176, 1262
VpaK11BI GGWCC 3 cut(s) 449, 598, 1248
VspI ATTAAT 1 cut(s) 525
XapI RAATTY 2 cut(s) 794, 1240
XceI RCATGY 1 cut(s) 1106
XhoI CTCGAG 1 cut(s) 356
XmaJI CCTAGG 1 cut(s) 214
XmiI GTMKAC 2 cut(s) 183, 761
XspI CTAG 3 cut(s) 215, 621, 1205
ZrmI AGTACT 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.