MD17G1089900.v1.1

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015387
Physical Location & Seq
Reverse (-)
7373850 .. 7377238
3389 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1089900.v1.1.491

Sequence Viewer

Length: 891 bp
ATGGCTGTTCCAGCTTTTGGAATAATGGCTTTTTCTCTGAGTGCTTTTGTGATTTTTGCATTGCTGGGTTTGGTCAGCTCAGCAAAATTTGAAGAGCTTTTCCAGCCAACCTGGGCTCTCGATCACTTCAACTATGAGGGAGAGCAAGTTCACATGAAACTCGACAACTTTTCCGGAGCTGGGTTTCAATCCAAGAACAAGTACTTGTTTGGAAAAGTGAGCATTCAGATTAAGCTCATCGAGGGCGACTCCGCCGGAACTGTCACTGCTTTCTATATGTCATCGGACGGTCCACTACACGATGAGTTCGACTTCGAGTTTCTGGGGAACACTACAGGGGAACCTTATTCAGTGCAGACCAATATATATGTCAATGGTGTGGGAAACAGAGAGCAAAGGTTGAACCTTTGGTTCGATCCCACCACGGAATTCCACTCCTACTCCATCTTCTGGAATCAGCGCCAAGTAGTGTTCCTAGTCGATGAGACGCCCATTAGAGTCCACACCAACATGGAAAACAAAGGGGTGCCCTTCCCCAAGGACCAAGCCATGGGTGTCTACAGTTCAATTTGGAATGCAGATGACTGGGCCACACAGGGTGGCAGGGTCAAGACAGATTGGTCCCATGCACCCTTCGTCGCAACCTACAAGGGCTTTGACATCAATGCCTGTGAGTGCCCAGTTTCTCTTGCAGCCGCAGATAAGGCAAAGAAGTGCAGCAGCAGTGGTGACCAGAAGTACTGGTGGGATGAACCTACTTTGTCCGAGCTCAATCTCCACCAGAACCACCAGCTCGTGTGGGTTAAGGCTCACCACATGGTCTATGACTACTGCACCGATTCCGCTAGGTTTCCGGTGACTCCACTCGAGTGCGTGCACCACCGCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016740 GO:0016757 GO:0016758 GO:0016762 GO:0046527
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

297

Amino Acids

33.59

Weight (kDa)

5.47

Isoelectric Point (pI)

34.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 31 - 208 1.4e-62 Glycosyl hydrolases family 16
XET_C PF06955 245 - 291 6.7e-17 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03210 AT4G03210
fragaria_vesca FvH4_6g43860 FvH4_6g43860
malus_domestica MD09G1102600.v1.1 MD17G1089900.v1.1
prunus_persica Prupe.3G223200_v2.0.a1
pyrus_communis pycom09g02590 pycom17g08700
rosa_chinensis RchiOBHm_Chr2g0160611
rosa_laevigata RLG00000021226
rosa_multiflora Rmu_co8450919.1_g000001
rosa_roxburghii Rroxscaffold_2G00089560
rosa_rugosa Rorug02G0484200
rosa_samantha Rh2DG572700
rosa_wichuraiana Rw2G045500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 619
AccB1I GGYRCC 1 cut(s) 526
AccB7I CCANNNNNTGG 3 cut(s) 17, 450, 550
AccI GTMKAC 1 cut(s) 558
AccIII TCCGGA 1 cut(s) 173
AciI CCGC 4 cut(s) 252, 696, 843, 883
AclWI GGATC 1 cut(s) 410
AcsI RAATTY 2 cut(s) 86, 428
AcyI GRCGYC 1 cut(s) 488
AdeI CACNNNGTG 1 cut(s) 599
AfaI GTAC 2 cut(s) 203, 740
AfiI CCNNNNNNNGG 4 cut(s) 17, 180, 450, 550
AgsI TTSAA 5 cut(s) 92, 130, 188, 403, 567
AjnI CCWGG 1 cut(s) 110
AleI CACNNNNGTG 1 cut(s) 868
AloI GAACNNNNNNTCC 2 cut(s) 132, 164
AluBI AGCT 7 cut(s) 14, 78, 97, 179, 235, 769, 793
AluI AGCT 7 cut(s) 14, 78, 97, 179, 235, 769, 793
Alw21I GWGCWC 2 cut(s) 771, 879
Alw26I GTCTC 1 cut(s) 479
Alw44I GTGCAC 1 cut(s) 875
AlwI GGATC 1 cut(s) 410
Ama87I CYCGRG 1 cut(s) 866
Aor13HI TCCGGA 1 cut(s) 173
AoxI GGCC 1 cut(s) 588
ApaLI GTGCAC 1 cut(s) 875
ApeKI GCWGC 3 cut(s) 692, 717, 720
ApoI RAATTY 2 cut(s) 86, 428
AspLEI GCGC 1 cut(s) 462
AspS9I GGNCC 4 cut(s) 290, 541, 588, 621
AsuHPI GGTGA 3 cut(s) 740, 803, 868
AvaI CYCGRG 1 cut(s) 866
AvaII GGWCC 3 cut(s) 290, 541, 621
BaeGI GKGCMC 3 cut(s) 531, 680, 879
BanI GGYRCC 1 cut(s) 526
BanII GRGCYC 2 cut(s) 118, 771
BauI CACGAG 1 cut(s) 794
Bbv12I GWGCWC 2 cut(s) 771, 879
BbvI GCAGC 3 cut(s) 704, 729, 732
BccI CCATC 1 cut(s) 452
BciT130I CCWGG 1 cut(s) 112
BcoDI GTCTC 1 cut(s) 479
BfaI CTAG 3 cut(s) 476, 846, 889
BfmI CTRYAG 2 cut(s) 333, 559
BfoI RGCGCY 1 cut(s) 463
BisI GCNGC 4 cut(s) 693, 696, 718, 721
BlpI GCTNAGC 1 cut(s) 79
BlsI GCNGC 4 cut(s) 694, 697, 719, 722
BmcAI AGTACT 2 cut(s) 203, 740
Bme1390I CCNGG 1 cut(s) 112
Bme18I GGWCC 3 cut(s) 290, 541, 621
BmeT110I CYCGRG 1 cut(s) 866
BmgT120I GGNCC 4 cut(s) 290, 541, 588, 621
BmiI GGNNCC 3 cut(s) 342, 528, 623
BmrFI CCNGG 1 cut(s) 112
BmrI ACTGGG 2 cut(s) 595, 674
BmuI ACTGGG 2 cut(s) 595, 674
BplI GAGNNNNNCTC 2 cut(s) 233, 265
Bpu1102I GCTNAGC 1 cut(s) 79
BsaHI GRCGYC 1 cut(s) 488
BsaJI CCNNGG 4 cut(s) 111, 423, 537, 549
BsaWI WCCGGW 2 cut(s) 173, 853
BsaXI ACNNNNNCTCC 4 cut(s) 132, 162, 425, 455
Bsc4I CCNNNNNNNGG 4 cut(s) 17, 180, 450, 550
Bse1I ACTGG 3 cut(s) 590, 680, 746
Bse3DI GCAATG 1 cut(s) 59
BseAI TCCGGA 1 cut(s) 173
BseBI CCWGG 1 cut(s) 112
BseDI CCNNGG 4 cut(s) 111, 423, 537, 549
BseGI GGATG 1 cut(s) 754
BseLI CCNNNNNNNGG 4 cut(s) 17, 180, 450, 550
BseMI GCAATG 1 cut(s) 59
BseMII CTCAG 2 cut(s) 29, 93
BseNI ACTGG 3 cut(s) 590, 680, 746
BseSI GKGCMC 3 cut(s) 531, 680, 879
BseXI GCAGC 3 cut(s) 704, 729, 732
BseYI CCCAGC 2 cut(s) 64, 179
BsgI GTGCAG 3 cut(s) 374, 736, 817
BshFI GGCC 1 cut(s) 590
BshNI GGYRCC 1 cut(s) 526
BsiHKAI GWGCWC 2 cut(s) 771, 879
BsiHKCI CYCGRG 1 cut(s) 866
BsiSI CCGG 3 cut(s) 174, 255, 854
BslFI GGGAC 1 cut(s) 607
BslI CCNNNNNNNGG 4 cut(s) 17, 180, 450, 550
BsmAI GTCTC 1 cut(s) 479
BsmBI CGTCTC 1 cut(s) 479
BsmFI GGGAC 1 cut(s) 607
BsmI GAATGC 2 cut(s) 222, 580
BsnI GGCC 1 cut(s) 590
BsoBI CYCGRG 1 cut(s) 866
Bsp1286I GDGCHC 5 cut(s) 118, 531, 680, 771, 879
Bsp13I TCCGGA 1 cut(s) 173
Bsp143I GATC 2 cut(s) 121, 415
Bsp1720I GCTNAGC 1 cut(s) 79
Bsp19I CCATGG 1 cut(s) 549
BspACI CCGC 4 cut(s) 252, 696, 843, 883
BspANI GGCC 1 cut(s) 590
BspCNI CTCAG 2 cut(s) 30, 92
BspEI TCCGGA 1 cut(s) 173
BspLI GGNNCC 3 cut(s) 342, 528, 623
BspPI GGATC 1 cut(s) 410
BspQI GCTCTTC 1 cut(s) 87
BspT107I GGYRCC 1 cut(s) 526
BsrDI GCAATG 1 cut(s) 59
BsrI ACTGG 3 cut(s) 590, 680, 746
BssECI CCNNGG 4 cut(s) 111, 423, 537, 549
BssMI GATC 2 cut(s) 121, 415
BssNI GRCGYC 1 cut(s) 488
BssSI CACGAG 1 cut(s) 794
BssT1I CCWWGG 2 cut(s) 537, 549
Bst2BI CACGAG 1 cut(s) 794
Bst2UI CCWGG 1 cut(s) 112
Bst4CI ACNGT 3 cut(s) 262, 290, 563
Bst6I CTCTTC 1 cut(s) 87
BstACI GRCGYC 1 cut(s) 488
BstC8I GCNNGC 1 cut(s) 875
BstDEI CTNAG 2 cut(s) 38, 79
BstDSI CCRYGG 2 cut(s) 423, 549
BstEII GGTNACC 1 cut(s) 728
BstF5I GGATG 1 cut(s) 754
BstH2I RGCGCY 1 cut(s) 463
BstHHI GCGC 1 cut(s) 462
BstKTI GATC 2 cut(s) 124, 418
BstMAI GTCTC 1 cut(s) 479
BstMBI GATC 2 cut(s) 121, 415
BstMWI GCNNNNNNNGC 3 cut(s) 11, 103, 704
BstNI CCWGG 1 cut(s) 112
BstPI GGTNACC 1 cut(s) 728
BstSCI CCNGG 1 cut(s) 110
BstSFI CTRYAG 2 cut(s) 333, 559
BstSLI GKGCMC 3 cut(s) 531, 680, 879
BstV1I GCAGC 3 cut(s) 704, 729, 732
BsuRI GGCC 1 cut(s) 590
BtgI CCRYGG 2 cut(s) 423, 549
BtsCI GGATG 1 cut(s) 754
BtsI GCAGTG 2 cut(s) 264, 730
BtsIMutI CAGTG 3 cut(s) 264, 357, 730
Cac8I GCNNGC 1 cut(s) 875
CfoI GCGC 1 cut(s) 462
Cfr13I GGNCC 4 cut(s) 290, 541, 588, 621
CseI GACGC 1 cut(s) 496
Csp6I GTAC 2 cut(s) 202, 739
CviAII CATG 5 cut(s) 154, 511, 550, 626, 817
CviQI GTAC 2 cut(s) 202, 739
DdeI CTNAG 2 cut(s) 38, 79
DpnI GATC 2 cut(s) 123, 417
DpnII GATC 2 cut(s) 121, 415
DraIII CACNNNGTG 1 cut(s) 599
DrdI GACNNNNNNGTC 1 cut(s) 619
DseDI GACNNNNNNGTC 1 cut(s) 619
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
EciI GGCGGA 1 cut(s) 241
Ecl136II GAGCTC 1 cut(s) 769
Eco130I CCWWGG 2 cut(s) 537, 549
Eco24I GRGCYC 2 cut(s) 118, 771
Eco47I GGWCC 3 cut(s) 290, 541, 621
Eco53kI GAGCTC 1 cut(s) 769
Eco88I CYCGRG 1 cut(s) 866
Eco91I GGTNACC 1 cut(s) 728
EcoICRI GAGCTC 1 cut(s) 769
EcoO65I GGTNACC 1 cut(s) 728
EcoRI GAATTC 1 cut(s) 428
EcoRII CCWGG 1 cut(s) 110
EcoT14I CCWWGG 2 cut(s) 537, 549
EcoT38I GRGCYC 2 cut(s) 118, 771
ErhI CCWWGG 2 cut(s) 537, 549
Esp3I CGTCTC 1 cut(s) 479
FaeI CATG 5 cut(s) 157, 514, 553, 629, 820
FaqI GGGAC 1 cut(s) 607
FatI CATG 5 cut(s) 153, 510, 549, 625, 816
FblI GTMKAC 1 cut(s) 558
Fnu4HI GCNGC 4 cut(s) 693, 696, 718, 721
FokI GGATG 1 cut(s) 761
FriOI GRGCYC 2 cut(s) 118, 771
Fsp4HI GCNGC 4 cut(s) 693, 696, 718, 721
FspBI CTAG 3 cut(s) 476, 846, 889
GlaI GCGC 1 cut(s) 461
GluI GCNGC 4 cut(s) 693, 696, 718, 721
GsaI CCCAGC 2 cut(s) 68, 183
HaeII RGCGCY 1 cut(s) 463
HaeIII GGCC 1 cut(s) 590
HapII CCGG 3 cut(s) 174, 255, 854
HgaI GACGC 1 cut(s) 496
HhaI GCGC 1 cut(s) 462
Hin1I GRCGYC 1 cut(s) 488
Hin1II CATG 5 cut(s) 157, 514, 553, 629, 820
Hin6I GCGC 1 cut(s) 460
HinP1I GCGC 1 cut(s) 460
HinfI GANTC 5 cut(s) 248, 454, 498, 839, 859
HpaII CCGG 3 cut(s) 174, 255, 854
HphI GGTGA 3 cut(s) 740, 803, 868
Hpy166II GTNNAC 5 cut(s) 151, 293, 502, 559, 877
Hpy188I TCNGA 4 cut(s) 39, 228, 286, 766
Hpy188III TCNNGA 4 cut(s) 119, 174, 451, 610
Hpy8I GTNNAC 5 cut(s) 151, 293, 502, 559, 877
Hpy99I CGWCG 1 cut(s) 641
HpyAV CCTTC 2 cut(s) 541, 643
HpyCH4III ACNGT 3 cut(s) 262, 290, 563
HpyCH4V TGCA 8 cut(s) 59, 355, 578, 629, 692, 717, 834, 877
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 103, 704
HpyF3I CTNAG 2 cut(s) 38, 79
Hsp92I GRCGYC 1 cut(s) 488
Hsp92II CATG 5 cut(s) 157, 514, 553, 629, 820
HspAI GCGC 1 cut(s) 460
Kpn2I TCCGGA 1 cut(s) 173
Kzo9I GATC 2 cut(s) 121, 415
LguI GCTCTTC 1 cut(s) 87
LmnI GCTCC 1 cut(s) 176
Lsp1109I GCAGC 3 cut(s) 704, 729, 732
MaeI CTAG 3 cut(s) 476, 846, 889
MaeIII GTNAC 3 cut(s) 262, 728, 856
MalI GATC 2 cut(s) 123, 417
MboI GATC 2 cut(s) 121, 415
MboII GAAGA 2 cut(s) 104, 439
MhlI GDGCHC 5 cut(s) 118, 531, 680, 771, 879
MluCI AATT 3 cut(s) 86, 428, 567
MlyI GAGTC 3 cut(s) 242, 507, 853
MnlI CCTC 2 cut(s) 130, 235
MroI TCCGGA 1 cut(s) 173
MseI TTAA 2 cut(s) 231, 804
MslI CAYNNNNRTG 2 cut(s) 509, 868
MspI CCGG 3 cut(s) 174, 255, 854
MspR9I CCNGG 1 cut(s) 112
Mva1269I GAATGC 2 cut(s) 222, 580
MvaI CCWGG 1 cut(s) 112
MwoI GCNNNNNNNGC 3 cut(s) 11, 103, 704
NcoI CCATGG 1 cut(s) 549
NdeII GATC 2 cut(s) 121, 415
NlaIII CATG 5 cut(s) 157, 514, 553, 629, 820
NlaIV GGNNCC 3 cut(s) 342, 528, 623
NmuCI GTSAC 3 cut(s) 262, 728, 856
OliI CACNNNNGTG 1 cut(s) 868
PaeR7I CTCGAG 1 cut(s) 866
PciSI GCTCTTC 1 cut(s) 87
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PctI GAATGC 2 cut(s) 222, 580
PfeI GAWTC 2 cut(s) 454, 839
PflMI CCANNNNNTGG 3 cut(s) 17, 450, 550
PkrI GCNGC 4 cut(s) 694, 697, 719, 722
PleI GAGTC 3 cut(s) 242, 506, 853
PpsI GAGTC 3 cut(s) 242, 506, 853
Psp124BI GAGCTC 1 cut(s) 771
Psp6I CCWGG 1 cut(s) 110
PspEI GGTNACC 1 cut(s) 728
PspFI CCCAGC 2 cut(s) 64, 179
PspGI CCWGG 1 cut(s) 110
PspN4I GGNNCC 3 cut(s) 342, 528, 623
PspPI GGNCC 4 cut(s) 290, 541, 588, 621
PspXI VCTCGAGB 1 cut(s) 866
RsaI GTAC 2 cut(s) 203, 740
RsaNI GTAC 2 cut(s) 202, 739
RseI CAYNNNNRTG 2 cut(s) 509, 868
SacI GAGCTC 1 cut(s) 771
SapI GCTCTTC 1 cut(s) 87
SaqAI TTAA 2 cut(s) 231, 804
SatI GCNGC 4 cut(s) 693, 696, 718, 721
Sau3AI GATC 2 cut(s) 121, 415
Sau96I GGNCC 4 cut(s) 290, 541, 588, 621
ScaI AGTACT 2 cut(s) 203, 740
SchI GAGTC 3 cut(s) 242, 507, 853
ScrFI CCNGG 1 cut(s) 112
SduI GDGCHC 5 cut(s) 118, 531, 680, 771, 879
SfcI CTRYAG 2 cut(s) 333, 559
Sfr274I CTCGAG 1 cut(s) 866
SinI GGWCC 3 cut(s) 290, 541, 621
SlaI CTCGAG 1 cut(s) 866
SmiMI CAYNNNNRTG 2 cut(s) 509, 868
SmlI CTYRAG 1 cut(s) 866
SmoI CTYRAG 1 cut(s) 866
Sse9I AATT 3 cut(s) 86, 428, 567
SsiI CCGC 4 cut(s) 252, 696, 843, 883
SspMI CTAG 3 cut(s) 476, 846, 889
SstI GAGCTC 1 cut(s) 771
StyD4I CCNGG 1 cut(s) 110
StyI CCWWGG 2 cut(s) 537, 549
TaaI ACNGT 3 cut(s) 262, 290, 563
TaqI TCGA 8 cut(s) 120, 162, 240, 309, 315, 414, 480, 867
TasI AATT 3 cut(s) 86, 428, 567
TatI WGTACW 2 cut(s) 201, 738
TauI GCSGC 1 cut(s) 698
TfiI GAWTC 2 cut(s) 454, 839
Tru1I TTAA 2 cut(s) 231, 804
Tru9I TTAA 2 cut(s) 231, 804
TscAI CASTG 3 cut(s) 271, 357, 730
TseFI GTSAC 3 cut(s) 262, 728, 856
TseI GCWGC 3 cut(s) 692, 717, 720
Tsp45I GTSAC 3 cut(s) 262, 728, 856
TspDTI ATGAA 2 cut(s) 170, 765
TspGWI ACGGA 1 cut(s) 440
TspRI CASTG 3 cut(s) 271, 357, 730
Van91I CCANNNNNTGG 3 cut(s) 17, 450, 550
VneI GTGCAC 1 cut(s) 875
VpaK11BI GGWCC 3 cut(s) 290, 541, 621
XapI RAATTY 2 cut(s) 86, 428
XhoI CTCGAG 1 cut(s) 866
XmiI GTMKAC 1 cut(s) 558
XspI CTAG 3 cut(s) 476, 846, 889
ZrmI AGTACT 2 cut(s) 203, 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.