Rh2DG572700

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0015387
Physical Location & Seq
Forward (+)
80296572 .. 80298170
1599 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG572700.1

Sequence Viewer

Length: 903 bp
ATGGCTACTACAGCCTCGAAAATCATGTCTCTGTCTCTCAGTGTGATTTTGGGTCTCTCATTGTTCTTGGGTCTGGTCAGCTCGGCCAAATTCGACCAGCTTTTCCAGCCCTACTGGGCCTCCGACCATTTCACATACGAAGGCGAGCTTCTTCATATGAAGCTCGACAATTTTTCCGGAGCTGGGTTTTCATCCAAGAACAAGTACATGTTTGGCAAAGTGACCGTACAGATTAAGCTCATAGAGGGTGACTCTGCTGGAACTGTCACTGCTTTCTATATGGCATCGGACGGTCCACTGCACAACGAGTTTGATTTCGAGTTCCTGGGCAACACCACAGGGGAGCCTTACTCTGTTCAGACCAACCTCTATATCAATGGCGTCGGTAATAGAGAGCAAAGGCTCGACCTTTGGTTTGACCCAACCACGGACTTCCACTCCTACTCCATTTTCTGGAACCAGCGCCAAGTAGTGTTTCTAGTGGATGAGACCCCAATTAGGGTACACACAAACATGGAAAGCAAGGGTTTGCCCTTCCCCAAGGACCAGGCTATGGGGGTATACAGCTCAATCTGGAATGCCGATGACTGGGCCACACAGGGCGGGCGGGTCAAGACCGATTGGTCCCATGGACCCTTTGTTGCATCTTATAAGGGCTTTGACATCAATGCCTGTGAGTGCCCGGTCTCCGTGGCCGGAGCCAATAACGCCAAGAAGTGCACCAGCAGCGCAGACAAGAAGTACTGGTGGGATGAGCCTGTGCTGTCCGAGCTGAACGTCCACCAGAACCACCAGCTGGTGTGGGTTAAGAACCACCACATGGTCTATGACTACTGCACTGATACTGCTAGGTTCCCAGTCACTCCAGTTGAGTGTGTGCACCACGGTCACCACCGCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016740 GO:0016757 GO:0016758 GO:0016762 GO:0046527
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

300

Amino Acids

33.94

Weight (kDa)

5.88

Isoelectric Point (pI)

30.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 33 - 209 1.3e-60 Glycosyl hydrolases family 16
XET_C PF06955 245 - 292 1.2e-17 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03210 AT4G03210
fragaria_vesca FvH4_6g43860 FvH4_6g43860
malus_domestica MD09G1102600.v1.1 MD17G1089900.v1.1
prunus_persica Prupe.3G223200_v2.0.a1
pyrus_communis pycom09g02590 pycom17g08700
rosa_chinensis RchiOBHm_Chr2g0160611
rosa_laevigata RLG00000021226
rosa_multiflora Rmu_co8450919.1_g000001
rosa_roxburghii Rroxscaffold_2G00089560
rosa_rugosa Rorug02G0484200
rosa_samantha Rh2DG572700
rosa_wichuraiana Rw2G045500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 651
AasI GACNNNNNNGTC 1 cut(s) 622
AccB7I CCANNNNNTGG 4 cut(s) 453, 553, 796, 820
AccI GTMKAC 1 cut(s) 561
AccIII TCCGGA 1 cut(s) 176
AciI CCGC 3 cut(s) 603, 607, 895
AcoI YGGCCR 2 cut(s) 84, 693
AcsI RAATTY 1 cut(s) 89
AcyI GRCGYC 1 cut(s) 381
AfaI GTAC 4 cut(s) 206, 228, 504, 743
AfiI CCNNNNNNNGG 9 cut(s) 183, 427, 453, 498, 499, 553, 588, 796, 820
AflIII ACRYGT 1 cut(s) 207
AjnI CCWGG 2 cut(s) 324, 546
AjuI GAANNNNNNNTTGG 2 cut(s) 459, 491
AluBI AGCT 9 cut(s) 81, 100, 148, 163, 182, 238, 567, 772, 796
AluI AGCT 9 cut(s) 81, 100, 148, 163, 182, 238, 567, 772, 796
Alw21I GWGCWC 2 cut(s) 722, 882
Alw26I GTCTC 5 cut(s) 33, 39, 59, 482, 691
Alw44I GTGCAC 2 cut(s) 718, 878
Aor13HI TCCGGA 1 cut(s) 176
AoxI GGCC 4 cut(s) 84, 117, 591, 693
ApaLI GTGCAC 2 cut(s) 718, 878
ApeKI GCWGC 1 cut(s) 726
ApoI RAATTY 1 cut(s) 89
ArsI GACNNNNNNTTYG 4 cut(s) 11, 43, 398, 430
AspLEI GCGC 2 cut(s) 465, 731
AspS9I GGNCC 6 cut(s) 117, 293, 544, 591, 624, 632
AsuC2I CCSGG 1 cut(s) 683
AsuHPI GGTGA 2 cut(s) 260, 881
AvaII GGWCC 4 cut(s) 293, 544, 624, 632
BaeGI GKGCMC 3 cut(s) 683, 722, 882
Bbv12I GWGCWC 2 cut(s) 722, 882
BbvI GCAGC 1 cut(s) 738
BciT130I CCWGG 2 cut(s) 326, 548
BcnI CCSGG 1 cut(s) 683
BcoDI GTCTC 5 cut(s) 33, 39, 59, 482, 691
BfaI CTAG 3 cut(s) 479, 849, 901
BfmI CTRYAG 1 cut(s) 9
BfoI RGCGCY 1 cut(s) 466
BisI GCNGC 1 cut(s) 727
BlsI GCNGC 1 cut(s) 728
BmcAI AGTACT 1 cut(s) 743
Bme1390I CCNGG 3 cut(s) 326, 548, 683
Bme18I GGWCC 4 cut(s) 293, 544, 624, 632
BmgT120I GGNCC 6 cut(s) 117, 293, 544, 591, 624, 632
BmiI GGNNCC 6 cut(s) 345, 458, 626, 634, 700, 854
BmrFI CCNGG 3 cut(s) 326, 548, 683
BmrI ACTGGG 3 cut(s) 124, 598, 851
BmsI GCATC 2 cut(s) 293, 653
BmuI ACTGGG 3 cut(s) 124, 598, 851
BplI GAGNNNNNCTC 4 cut(s) 236, 268, 335, 367
BpmI CTGGAG 1 cut(s) 849
BpuMI CCSGG 1 cut(s) 683
BsaHI GRCGYC 1 cut(s) 381
BsaI GGTCTC 3 cut(s) 59, 482, 691
BsaJI CCNNGG 6 cut(s) 325, 426, 540, 628, 690, 883
BsaWI WCCGGW 1 cut(s) 176
BsaXI ACNNNNNCTCC 6 cut(s) 104, 134, 422, 428, 452, 458
Bsc4I CCNNNNNNNGG 9 cut(s) 183, 427, 453, 498, 499, 553, 588, 796, 820
Bse1I ACTGG 5 cut(s) 119, 593, 749, 857, 866
BseAI TCCGGA 1 cut(s) 176
BseBI CCWGG 2 cut(s) 326, 548
BseDI CCNNGG 6 cut(s) 325, 426, 540, 628, 690, 883
BseGI GGATG 3 cut(s) 191, 490, 757
BseLI CCNNNNNNNGG 9 cut(s) 183, 427, 453, 498, 499, 553, 588, 796, 820
BseMII CTCAG 1 cut(s) 52
BseNI ACTGG 5 cut(s) 119, 593, 749, 857, 866
BseSI GKGCMC 3 cut(s) 683, 722, 882
BseXI GCAGC 1 cut(s) 738
BseYI CCCAGC 1 cut(s) 182
BsgI GTGCAG 2 cut(s) 284, 820
BshFI GGCC 4 cut(s) 86, 119, 593, 695
BsiHKAI GWGCWC 2 cut(s) 722, 882
BsiSI CCGG 3 cut(s) 177, 683, 696
BslFI GGGAC 1 cut(s) 610
BslI CCNNNNNNNGG 9 cut(s) 183, 427, 453, 498, 499, 553, 588, 796, 820
BsmAI GTCTC 5 cut(s) 33, 39, 59, 482, 691
BsmFI GGGAC 1 cut(s) 610
BsmI GAATGC 1 cut(s) 583
BsnI GGCC 4 cut(s) 86, 119, 593, 695
Bso31I GGTCTC 3 cut(s) 59, 482, 691
Bsp1286I GDGCHC 3 cut(s) 683, 722, 882
Bsp13I TCCGGA 1 cut(s) 176
Bsp19I CCATGG 1 cut(s) 628
BspACI CCGC 3 cut(s) 603, 607, 895
BspANI GGCC 4 cut(s) 86, 119, 593, 695
BspCNI CTCAG 1 cut(s) 51
BspEI TCCGGA 1 cut(s) 176
BspLI GGNNCC 6 cut(s) 345, 458, 626, 634, 700, 854
BspTNI GGTCTC 3 cut(s) 59, 482, 691
BsrI ACTGG 5 cut(s) 119, 593, 749, 857, 866
BssECI CCNNGG 6 cut(s) 325, 426, 540, 628, 690, 883
BssNAI GTATAC 1 cut(s) 562
BssNI GRCGYC 1 cut(s) 381
BssT1I CCWWGG 2 cut(s) 540, 628
Bst1107I GTATAC 1 cut(s) 562
Bst2UI CCWGG 2 cut(s) 326, 548
Bst4CI ACNGT 4 cut(s) 226, 265, 293, 887
BstACI GRCGYC 1 cut(s) 381
BstC8I GCNNGC 2 cut(s) 146, 605
BstDEI CTNAG 1 cut(s) 38
BstDSI CCRYGG 4 cut(s) 426, 628, 690, 883
BstEII GGTNACC 1 cut(s) 887
BstF5I GGATG 3 cut(s) 191, 490, 757
BstH2I RGCGCY 1 cut(s) 466
BstHHI GCGC 2 cut(s) 465, 731
BstMAI GTCTC 5 cut(s) 33, 39, 59, 482, 691
BstMWI GCNNNNNNNGC 5 cut(s) 11, 106, 707, 726, 769
BstNI CCWGG 2 cut(s) 326, 548
BstNSI RCATGY 1 cut(s) 211
BstPI GGTNACC 1 cut(s) 887
BstSCI CCNGG 3 cut(s) 324, 546, 681
BstSFI CTRYAG 1 cut(s) 9
BstSLI GKGCMC 3 cut(s) 683, 722, 882
BstV1I GCAGC 1 cut(s) 738
BstZ17I GTATAC 1 cut(s) 562
BsuRI GGCC 4 cut(s) 86, 119, 593, 695
BtgI CCRYGG 4 cut(s) 426, 628, 690, 883
BtsCI GGATG 3 cut(s) 191, 490, 757
BtsI GCAGTG 2 cut(s) 267, 296
BtsIMutI CAGTG 4 cut(s) 46, 267, 296, 837
Cac8I GCNNGC 2 cut(s) 146, 605
CfoI GCGC 2 cut(s) 465, 731
Cfr13I GGNCC 6 cut(s) 117, 293, 544, 591, 624, 632
CseI GACGC 1 cut(s) 370
Csp6I GTAC 4 cut(s) 205, 227, 503, 742
CviAII CATG 5 cut(s) 25, 208, 514, 629, 820
CviQI GTAC 4 cut(s) 205, 227, 503, 742
DdeI CTNAG 1 cut(s) 38
DrdI GACNNNNNNGTC 1 cut(s) 622
DseDI GACNNNNNNGTC 1 cut(s) 622
EaeI YGGCCR 2 cut(s) 84, 693
Eco130I CCWWGG 2 cut(s) 540, 628
Eco31I GGTCTC 3 cut(s) 59, 482, 691
Eco47I GGWCC 4 cut(s) 293, 544, 624, 632
Eco91I GGTNACC 1 cut(s) 887
EcoO65I GGTNACC 1 cut(s) 887
EcoRII CCWGG 2 cut(s) 324, 546
EcoT14I CCWWGG 2 cut(s) 540, 628
ErhI CCWWGG 2 cut(s) 540, 628
FaeI CATG 5 cut(s) 28, 211, 517, 632, 823
FalI AAGNNNNNCTT 2 cut(s) 132, 164
FaqI GGGAC 1 cut(s) 610
FatI CATG 5 cut(s) 24, 207, 513, 628, 819
FauI CCCGC 2 cut(s) 596, 600
FauNDI CATATG 1 cut(s) 156
FblI GTMKAC 1 cut(s) 561
Fnu4HI GCNGC 1 cut(s) 727
FokI GGATG 3 cut(s) 178, 497, 764
Fsp4HI GCNGC 1 cut(s) 727
FspBI CTAG 3 cut(s) 479, 849, 901
GlaI GCGC 2 cut(s) 464, 730
GluI GCNGC 1 cut(s) 727
GsaI CCCAGC 1 cut(s) 186
GsuI CTGGAG 1 cut(s) 849
HaeII RGCGCY 1 cut(s) 466
HaeIII GGCC 4 cut(s) 86, 119, 593, 695
HapII CCGG 3 cut(s) 177, 683, 696
HgaI GACGC 1 cut(s) 370
HhaI GCGC 2 cut(s) 465, 731
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 5 cut(s) 28, 211, 517, 632, 823
Hin6I GCGC 2 cut(s) 463, 729
HinP1I GCGC 2 cut(s) 463, 729
HinfI GANTC 1 cut(s) 251
HpaII CCGG 3 cut(s) 177, 683, 696
HphI GGTGA 2 cut(s) 260, 881
Hpy166II GTNNAC 6 cut(s) 296, 505, 562, 720, 781, 880
Hpy188I TCNGA 4 cut(s) 124, 289, 360, 769
Hpy188III TCNNGA 4 cut(s) 177, 454, 574, 613
Hpy8I GTNNAC 6 cut(s) 296, 505, 562, 720, 781, 880
Hpy99I CGWCG 1 cut(s) 386
HpyAV CCTTC 2 cut(s) 134, 544
HpyCH4III ACNGT 4 cut(s) 226, 265, 293, 887
HpyCH4IV ACGT 1 cut(s) 777
HpyCH4V TGCA 5 cut(s) 301, 644, 720, 837, 880
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 106, 707, 726, 769
HpyF3I CTNAG 1 cut(s) 38
HpySE526I ACGT 1 cut(s) 777
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 5 cut(s) 28, 211, 517, 632, 823
HspAI GCGC 2 cut(s) 463, 729
Kpn2I TCCGGA 1 cut(s) 176
LmnI GCTCC 3 cut(s) 179, 343, 698
Lsp1109I GCAGC 1 cut(s) 738
LweI GCATC 2 cut(s) 293, 653
MaeI CTAG 3 cut(s) 479, 849, 901
MaeII ACGT 1 cut(s) 777
MaeIII GTNAC 5 cut(s) 220, 248, 265, 859, 887
MboII GAAGA 1 cut(s) 143
MhlI GDGCHC 3 cut(s) 683, 722, 882
MluCI AATT 3 cut(s) 89, 169, 495
MlyI GAGTC 1 cut(s) 245
MmeI TCCRAC 1 cut(s) 147
MnlI CCTC 4 cut(s) 25, 130, 238, 377
MroI TCCGGA 1 cut(s) 176
MseI TTAA 2 cut(s) 234, 807
MslI CAYNNNNRTG 1 cut(s) 512
MspA1I CMGCKG 1 cut(s) 796
MspI CCGG 3 cut(s) 177, 683, 696
MspR9I CCNGG 3 cut(s) 326, 548, 683
Mva1269I GAATGC 1 cut(s) 583
MvaI CCWGG 2 cut(s) 326, 548
MwoI GCNNNNNNNGC 5 cut(s) 11, 106, 707, 726, 769
NciI CCSGG 1 cut(s) 683
NcoI CCATGG 1 cut(s) 628
NdeI CATATG 1 cut(s) 156
NlaIII CATG 5 cut(s) 28, 211, 517, 632, 823
NlaIV GGNNCC 6 cut(s) 345, 458, 626, 634, 700, 854
NmeAIII GCCGAG 1 cut(s) 62
NmuCI GTSAC 5 cut(s) 220, 248, 265, 859, 887
NspI RCATGY 1 cut(s) 211
PciI ACATGT 1 cut(s) 207
PctI GAATGC 1 cut(s) 583
PflMI CCANNNNNTGG 4 cut(s) 453, 553, 796, 820
PkrI GCNGC 1 cut(s) 728
PleI GAGTC 1 cut(s) 245
PpsI GAGTC 1 cut(s) 245
PscI ACATGT 1 cut(s) 207
PsiI TTATAA 1 cut(s) 651
Psp6I CCWGG 2 cut(s) 324, 546
PspEI GGTNACC 1 cut(s) 887
PspFI CCCAGC 1 cut(s) 182
PspGI CCWGG 2 cut(s) 324, 546
PspN4I GGNNCC 6 cut(s) 345, 458, 626, 634, 700, 854
PspPI GGNCC 6 cut(s) 117, 293, 544, 591, 624, 632
PvuII CAGCTG 1 cut(s) 796
RsaI GTAC 4 cut(s) 206, 228, 504, 743
RsaNI GTAC 4 cut(s) 205, 227, 503, 742
RseI CAYNNNNRTG 1 cut(s) 512
SaqAI TTAA 2 cut(s) 234, 807
SatI GCNGC 1 cut(s) 727
Sau96I GGNCC 6 cut(s) 117, 293, 544, 591, 624, 632
ScaI AGTACT 1 cut(s) 743
SchI GAGTC 1 cut(s) 245
ScrFI CCNGG 3 cut(s) 326, 548, 683
SduI GDGCHC 3 cut(s) 683, 722, 882
SfaNI GCATC 2 cut(s) 293, 653
SfcI CTRYAG 1 cut(s) 9
SinI GGWCC 4 cut(s) 293, 544, 624, 632
SmiMI CAYNNNNRTG 1 cut(s) 512
Sse9I AATT 3 cut(s) 89, 169, 495
SsiI CCGC 3 cut(s) 603, 607, 895
SspMI CTAG 3 cut(s) 479, 849, 901
StyD4I CCNGG 3 cut(s) 324, 546, 681
StyI CCWWGG 2 cut(s) 540, 628
TaaI ACNGT 4 cut(s) 226, 265, 293, 887
TaiI ACGT 1 cut(s) 780
TaqI TCGA 5 cut(s) 17, 93, 165, 318, 405
TaqII GACCGA 1 cut(s) 632
TasI AATT 3 cut(s) 89, 169, 495
TatI WGTACW 2 cut(s) 204, 741
Tru1I TTAA 2 cut(s) 234, 807
Tru9I TTAA 2 cut(s) 234, 807
TscAI CASTG 4 cut(s) 46, 274, 303, 844
TseFI GTSAC 5 cut(s) 220, 248, 265, 859, 887
TseI GCWGC 1 cut(s) 726
Tsp45I GTSAC 5 cut(s) 220, 248, 265, 859, 887
TspDTI ATGAA 3 cut(s) 143, 173, 180
TspGWI ACGGA 2 cut(s) 443, 679
TspRI CASTG 4 cut(s) 46, 274, 303, 844
Van91I CCANNNNNTGG 4 cut(s) 453, 553, 796, 820
VneI GTGCAC 2 cut(s) 718, 878
VpaK11BI GGWCC 4 cut(s) 293, 544, 624, 632
XapI RAATTY 1 cut(s) 89
XceI RCATGY 1 cut(s) 211
XmiI GTMKAC 1 cut(s) 561
XspI CTAG 3 cut(s) 479, 849, 901
ZrmI AGTACT 1 cut(s) 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.