Rw2G045500

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0015387
Physical Location & Seq
Forward (+)
72478937 .. 72481327
2391 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G045500.1

Sequence Viewer

Length: 909 bp
ATGGCTACTACTACTTCAGCCTCGAAAGTCATGTCTCTGTCTCTCAGTGTGATTTTGGGTCTCTCATTGTTCTTGGGTCTGGTCAGCTCGGCCAAATTCGACCAGCTTTTCCAGCCCTACTGGGCCTCCGACCATTTCACATACGAAGGCGAGCTTCTTCATATGAAGCTCGACAATTTTTCCGGAGCTGGGTTTTCATCCAAGAACAAGTACATGTTTGGCAAAGTGACCGTACAGATTAAGCTCATAGAGGGTGACTCTGCTGGAACTGTCACTGCTTTCTATATGTCATCGGACGGTCCACTGCACAACGAATTTGATTTCGAGTTCCTGGGCAACACCACAGGGGAGCCTTACTCTGTTCAGACCAACCTCTATATCAATGGCGTCGGCAATAGAGAGCAAAGGCTCGACCTTTGGTTTGACCCAACCACGGACTTCCATTCCTACTCCATTTTCTGGAACCAGCGCCAAGTAGTGTTTCTAGTGGATGAGACCCCAATTAGGGTACACACAAACATGGAAAGCAAGGGTTTGCCCTTCCCCAAGGACCAGGCTATGGGGGTATACAGCTCAATCTGGAATGCCGATGACTGGGCCACACAGGGCGGGCGGGTCAAGACCGATTGGACCCATGGACCCTTTGTTGCATCTTATAAGGGCTTTGACATCAATGCCTGTGAGTGCCCGGTCTCCGTGGCCGGAGCCGATAACGCCAAGAAGTGCACCAGCAGCGCAGACAAGAAGTACTGGTGGGATGAGCCTGTGCTGTCCGAGCTGAACGTCCACCAGAACCACCAGCTGGTGTGGGTTAAGAACCACCACATGGTCTATGACTACTGCACTGATACTGCTAGGTTCCCAGTCACTCCAGTTGAGTGTGTGCACCACGGTCACCACCGCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016740 GO:0016757 GO:0016758 GO:0016762 GO:0046527
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

302

Amino Acids

34.15

Weight (kDa)

5.79

Isoelectric Point (pI)

31.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 35 - 211 7.7e-61 Glycosyl hydrolases family 16
XET_C PF06955 247 - 294 1.2e-17 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03210 AT4G03210
fragaria_vesca FvH4_6g43860 FvH4_6g43860
malus_domestica MD09G1102600.v1.1 MD17G1089900.v1.1
prunus_persica Prupe.3G223200_v2.0.a1
pyrus_communis pycom09g02590 pycom17g08700
rosa_chinensis RchiOBHm_Chr2g0160611
rosa_laevigata RLG00000021226
rosa_multiflora Rmu_co8450919.1_g000001
rosa_roxburghii Rroxscaffold_2G00089560
rosa_rugosa Rorug02G0484200
rosa_samantha Rh2DG572700
rosa_wichuraiana Rw2G045500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 657
AccB7I CCANNNNNTGG 4 cut(s) 459, 559, 802, 826
AccI GTMKAC 1 cut(s) 567
AccIII TCCGGA 1 cut(s) 182
AciI CCGC 3 cut(s) 609, 613, 901
AcoI YGGCCR 2 cut(s) 90, 699
AcsI RAATTY 2 cut(s) 95, 314
AcyI GRCGYC 1 cut(s) 387
AfaI GTAC 4 cut(s) 212, 234, 510, 749
AfiI CCNNNNNNNGG 9 cut(s) 189, 433, 459, 504, 505, 559, 594, 802, 826
AflIII ACRYGT 1 cut(s) 213
AjnI CCWGG 2 cut(s) 330, 552
AjuI GAANNNNNNNTTGG 2 cut(s) 465, 497
AluBI AGCT 9 cut(s) 87, 106, 154, 169, 188, 244, 573, 778, 802
AluI AGCT 9 cut(s) 87, 106, 154, 169, 188, 244, 573, 778, 802
Alw21I GWGCWC 2 cut(s) 728, 888
Alw26I GTCTC 5 cut(s) 39, 45, 65, 488, 697
Alw44I GTGCAC 2 cut(s) 724, 884
Aor13HI TCCGGA 1 cut(s) 182
AoxI GGCC 4 cut(s) 90, 123, 597, 699
ApaLI GTGCAC 2 cut(s) 724, 884
ApeKI GCWGC 1 cut(s) 732
ApoI RAATTY 2 cut(s) 95, 314
ArsI GACNNNNNNTTYG 4 cut(s) 17, 49, 404, 436
AspLEI GCGC 2 cut(s) 471, 737
AspS9I GGNCC 6 cut(s) 123, 299, 550, 597, 630, 638
AsuC2I CCSGG 1 cut(s) 689
AsuHPI GGTGA 2 cut(s) 266, 887
AvaII GGWCC 4 cut(s) 299, 550, 630, 638
BaeGI GKGCMC 3 cut(s) 689, 728, 888
Bbv12I GWGCWC 2 cut(s) 728, 888
BbvI GCAGC 1 cut(s) 744
BciT130I CCWGG 2 cut(s) 332, 554
BcnI CCSGG 1 cut(s) 689
BcoDI GTCTC 5 cut(s) 39, 45, 65, 488, 697
BfaI CTAG 3 cut(s) 485, 855, 907
BfoI RGCGCY 1 cut(s) 472
BisI GCNGC 1 cut(s) 733
BlsI GCNGC 1 cut(s) 734
BmcAI AGTACT 1 cut(s) 749
Bme1390I CCNGG 3 cut(s) 332, 554, 689
Bme18I GGWCC 4 cut(s) 299, 550, 630, 638
BmgT120I GGNCC 6 cut(s) 123, 299, 550, 597, 630, 638
BmiI GGNNCC 6 cut(s) 351, 464, 632, 640, 706, 860
BmrFI CCNGG 3 cut(s) 332, 554, 689
BmrI ACTGGG 3 cut(s) 130, 604, 857
BmsI GCATC 1 cut(s) 659
BmuI ACTGGG 3 cut(s) 130, 604, 857
BplI GAGNNNNNCTC 4 cut(s) 242, 274, 341, 373
BpmI CTGGAG 1 cut(s) 855
BpuMI CCSGG 1 cut(s) 689
BsaHI GRCGYC 1 cut(s) 387
BsaI GGTCTC 3 cut(s) 65, 488, 697
BsaJI CCNNGG 6 cut(s) 331, 432, 546, 634, 696, 889
BsaWI WCCGGW 1 cut(s) 182
BsaXI ACNNNNNCTCC 2 cut(s) 110, 140
Bsc4I CCNNNNNNNGG 9 cut(s) 189, 433, 459, 504, 505, 559, 594, 802, 826
Bse1I ACTGG 5 cut(s) 125, 599, 755, 863, 872
BseAI TCCGGA 1 cut(s) 182
BseBI CCWGG 2 cut(s) 332, 554
BseDI CCNNGG 6 cut(s) 331, 432, 546, 634, 696, 889
BseGI GGATG 3 cut(s) 197, 496, 763
BseLI CCNNNNNNNGG 9 cut(s) 189, 433, 459, 504, 505, 559, 594, 802, 826
BseMII CTCAG 1 cut(s) 58
BseNI ACTGG 5 cut(s) 125, 599, 755, 863, 872
BseSI GKGCMC 3 cut(s) 689, 728, 888
BseXI GCAGC 1 cut(s) 744
BseYI CCCAGC 1 cut(s) 188
BsgI GTGCAG 2 cut(s) 290, 826
BshFI GGCC 4 cut(s) 92, 125, 599, 701
BsiHKAI GWGCWC 2 cut(s) 728, 888
BsiSI CCGG 3 cut(s) 183, 689, 702
BslI CCNNNNNNNGG 9 cut(s) 189, 433, 459, 504, 505, 559, 594, 802, 826
BsmAI GTCTC 5 cut(s) 39, 45, 65, 488, 697
BsmI GAATGC 1 cut(s) 589
BsnI GGCC 4 cut(s) 92, 125, 599, 701
Bso31I GGTCTC 3 cut(s) 65, 488, 697
Bsp1286I GDGCHC 3 cut(s) 689, 728, 888
Bsp13I TCCGGA 1 cut(s) 182
Bsp19I CCATGG 1 cut(s) 634
BspACI CCGC 3 cut(s) 609, 613, 901
BspANI GGCC 4 cut(s) 92, 125, 599, 701
BspCNI CTCAG 1 cut(s) 57
BspEI TCCGGA 1 cut(s) 182
BspLI GGNNCC 6 cut(s) 351, 464, 632, 640, 706, 860
BspTNI GGTCTC 3 cut(s) 65, 488, 697
BsrI ACTGG 5 cut(s) 125, 599, 755, 863, 872
BssECI CCNNGG 6 cut(s) 331, 432, 546, 634, 696, 889
BssNAI GTATAC 1 cut(s) 568
BssNI GRCGYC 1 cut(s) 387
BssT1I CCWWGG 2 cut(s) 546, 634
Bst1107I GTATAC 1 cut(s) 568
Bst2UI CCWGG 2 cut(s) 332, 554
Bst4CI ACNGT 4 cut(s) 232, 271, 299, 893
BstACI GRCGYC 1 cut(s) 387
BstC8I GCNNGC 2 cut(s) 152, 611
BstDEI CTNAG 1 cut(s) 44
BstDSI CCRYGG 4 cut(s) 432, 634, 696, 889
BstEII GGTNACC 1 cut(s) 893
BstF5I GGATG 3 cut(s) 197, 496, 763
BstH2I RGCGCY 1 cut(s) 472
BstHHI GCGC 2 cut(s) 471, 737
BstMAI GTCTC 5 cut(s) 39, 45, 65, 488, 697
BstMWI GCNNNNNNNGC 4 cut(s) 112, 713, 732, 775
BstNI CCWGG 2 cut(s) 332, 554
BstNSI RCATGY 1 cut(s) 217
BstPI GGTNACC 1 cut(s) 893
BstSCI CCNGG 3 cut(s) 330, 552, 687
BstSLI GKGCMC 3 cut(s) 689, 728, 888
BstV1I GCAGC 1 cut(s) 744
BstZ17I GTATAC 1 cut(s) 568
BsuRI GGCC 4 cut(s) 92, 125, 599, 701
BtgI CCRYGG 4 cut(s) 432, 634, 696, 889
BtsCI GGATG 3 cut(s) 197, 496, 763
BtsI GCAGTG 2 cut(s) 273, 302
BtsIMutI CAGTG 4 cut(s) 52, 273, 302, 843
Cac8I GCNNGC 2 cut(s) 152, 611
CfoI GCGC 2 cut(s) 471, 737
Cfr13I GGNCC 6 cut(s) 123, 299, 550, 597, 630, 638
CseI GACGC 1 cut(s) 376
Csp6I GTAC 4 cut(s) 211, 233, 509, 748
CviAII CATG 5 cut(s) 31, 214, 520, 635, 826
CviQI GTAC 4 cut(s) 211, 233, 509, 748
DdeI CTNAG 1 cut(s) 44
EaeI YGGCCR 2 cut(s) 90, 699
Eco130I CCWWGG 2 cut(s) 546, 634
Eco31I GGTCTC 3 cut(s) 65, 488, 697
Eco47I GGWCC 4 cut(s) 299, 550, 630, 638
Eco91I GGTNACC 1 cut(s) 893
EcoO65I GGTNACC 1 cut(s) 893
EcoRII CCWGG 2 cut(s) 330, 552
EcoT14I CCWWGG 2 cut(s) 546, 634
ErhI CCWWGG 2 cut(s) 546, 634
FaeI CATG 5 cut(s) 34, 217, 523, 638, 829
FalI AAGNNNNNCTT 2 cut(s) 138, 170
FatI CATG 5 cut(s) 30, 213, 519, 634, 825
FauI CCCGC 2 cut(s) 602, 606
FauNDI CATATG 1 cut(s) 162
FblI GTMKAC 1 cut(s) 567
Fnu4HI GCNGC 1 cut(s) 733
FokI GGATG 3 cut(s) 184, 503, 770
Fsp4HI GCNGC 1 cut(s) 733
FspBI CTAG 3 cut(s) 485, 855, 907
GlaI GCGC 2 cut(s) 470, 736
GluI GCNGC 1 cut(s) 733
GsaI CCCAGC 1 cut(s) 192
GsuI CTGGAG 1 cut(s) 855
HaeII RGCGCY 1 cut(s) 472
HaeIII GGCC 4 cut(s) 92, 125, 599, 701
HapII CCGG 3 cut(s) 183, 689, 702
HgaI GACGC 1 cut(s) 376
HhaI GCGC 2 cut(s) 471, 737
Hin1I GRCGYC 1 cut(s) 387
Hin1II CATG 5 cut(s) 34, 217, 523, 638, 829
Hin6I GCGC 2 cut(s) 469, 735
HinP1I GCGC 2 cut(s) 469, 735
HinfI GANTC 1 cut(s) 257
HpaII CCGG 3 cut(s) 183, 689, 702
HphI GGTGA 2 cut(s) 266, 887
Hpy166II GTNNAC 6 cut(s) 302, 511, 568, 726, 787, 886
Hpy188I TCNGA 4 cut(s) 130, 295, 366, 775
Hpy188III TCNNGA 4 cut(s) 183, 460, 580, 619
Hpy8I GTNNAC 6 cut(s) 302, 511, 568, 726, 787, 886
Hpy99I CGWCG 1 cut(s) 392
HpyAV CCTTC 2 cut(s) 140, 550
HpyCH4III ACNGT 4 cut(s) 232, 271, 299, 893
HpyCH4IV ACGT 1 cut(s) 783
HpyCH4V TGCA 5 cut(s) 307, 650, 726, 843, 886
HpyF10VI GCNNNNNNNGC 4 cut(s) 112, 713, 732, 775
HpyF3I CTNAG 1 cut(s) 44
HpySE526I ACGT 1 cut(s) 783
Hsp92I GRCGYC 1 cut(s) 387
Hsp92II CATG 5 cut(s) 34, 217, 523, 638, 829
HspAI GCGC 2 cut(s) 469, 735
Kpn2I TCCGGA 1 cut(s) 182
LmnI GCTCC 3 cut(s) 185, 349, 704
Lsp1109I GCAGC 1 cut(s) 744
LweI GCATC 1 cut(s) 659
MaeI CTAG 3 cut(s) 485, 855, 907
MaeII ACGT 1 cut(s) 783
MaeIII GTNAC 5 cut(s) 226, 254, 271, 865, 893
MboII GAAGA 1 cut(s) 149
MhlI GDGCHC 3 cut(s) 689, 728, 888
MluCI AATT 4 cut(s) 95, 175, 314, 501
MlyI GAGTC 1 cut(s) 251
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 4 cut(s) 31, 136, 244, 383
MroI TCCGGA 1 cut(s) 182
MseI TTAA 2 cut(s) 240, 813
MslI CAYNNNNRTG 1 cut(s) 518
MspA1I CMGCKG 1 cut(s) 802
MspI CCGG 3 cut(s) 183, 689, 702
MspR9I CCNGG 3 cut(s) 332, 554, 689
Mva1269I GAATGC 1 cut(s) 589
MvaI CCWGG 2 cut(s) 332, 554
MwoI GCNNNNNNNGC 4 cut(s) 112, 713, 732, 775
NciI CCSGG 1 cut(s) 689
NcoI CCATGG 1 cut(s) 634
NdeI CATATG 1 cut(s) 162
NlaIII CATG 5 cut(s) 34, 217, 523, 638, 829
NlaIV GGNNCC 6 cut(s) 351, 464, 632, 640, 706, 860
NmeAIII GCCGAG 1 cut(s) 68
NmuCI GTSAC 5 cut(s) 226, 254, 271, 865, 893
NspI RCATGY 1 cut(s) 217
PciI ACATGT 1 cut(s) 213
PctI GAATGC 1 cut(s) 589
PflMI CCANNNNNTGG 4 cut(s) 459, 559, 802, 826
PkrI GCNGC 1 cut(s) 734
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
PscI ACATGT 1 cut(s) 213
PsiI TTATAA 1 cut(s) 657
Psp6I CCWGG 2 cut(s) 330, 552
PspEI GGTNACC 1 cut(s) 893
PspFI CCCAGC 1 cut(s) 188
PspGI CCWGG 2 cut(s) 330, 552
PspN4I GGNNCC 6 cut(s) 351, 464, 632, 640, 706, 860
PspPI GGNCC 6 cut(s) 123, 299, 550, 597, 630, 638
PvuII CAGCTG 1 cut(s) 802
RsaI GTAC 4 cut(s) 212, 234, 510, 749
RsaNI GTAC 4 cut(s) 211, 233, 509, 748
RseI CAYNNNNRTG 1 cut(s) 518
SaqAI TTAA 2 cut(s) 240, 813
SatI GCNGC 1 cut(s) 733
Sau96I GGNCC 6 cut(s) 123, 299, 550, 597, 630, 638
ScaI AGTACT 1 cut(s) 749
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 3 cut(s) 332, 554, 689
SduI GDGCHC 3 cut(s) 689, 728, 888
SfaNI GCATC 1 cut(s) 659
SinI GGWCC 4 cut(s) 299, 550, 630, 638
SmiMI CAYNNNNRTG 1 cut(s) 518
Sse9I AATT 4 cut(s) 95, 175, 314, 501
SsiI CCGC 3 cut(s) 609, 613, 901
SspMI CTAG 3 cut(s) 485, 855, 907
StyD4I CCNGG 3 cut(s) 330, 552, 687
StyI CCWWGG 2 cut(s) 546, 634
TaaI ACNGT 4 cut(s) 232, 271, 299, 893
TaiI ACGT 1 cut(s) 786
TaqI TCGA 5 cut(s) 23, 99, 171, 324, 411
TaqII GACCGA 1 cut(s) 638
TasI AATT 4 cut(s) 95, 175, 314, 501
TatI WGTACW 2 cut(s) 210, 747
Tru1I TTAA 2 cut(s) 240, 813
Tru9I TTAA 2 cut(s) 240, 813
TscAI CASTG 4 cut(s) 52, 280, 309, 850
TseFI GTSAC 5 cut(s) 226, 254, 271, 865, 893
TseI GCWGC 1 cut(s) 732
Tsp45I GTSAC 5 cut(s) 226, 254, 271, 865, 893
TspDTI ATGAA 3 cut(s) 149, 179, 186
TspGWI ACGGA 2 cut(s) 449, 685
TspRI CASTG 4 cut(s) 52, 280, 309, 850
Van91I CCANNNNNTGG 4 cut(s) 459, 559, 802, 826
VneI GTGCAC 2 cut(s) 724, 884
VpaK11BI GGWCC 4 cut(s) 299, 550, 630, 638
XapI RAATTY 2 cut(s) 95, 314
XceI RCATGY 1 cut(s) 217
XmiI GTMKAC 1 cut(s) 567
XspI CTAG 3 cut(s) 485, 855, 907
ZrmI AGTACT 1 cut(s) 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.