pycom09g02590

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0015387
Physical Location & Seq
Reverse (-)
1936336 .. 1938707
2372 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g02590.2

Sequence Viewer

Length: 891 bp
ATGGCTGGTTCAGCTTTTGGAATAATGGCTTTTTCTCTGAGTGCTTTTCTGAGTCTTGCATTGCTGGGTTTGGTCAGCTCAGCAAAATTTGATGAGCTTTTCCAGCCAACCTGGGCTTTCGATCATTTCACCTACGAGGGAGAGCAAATTCACATGAAACTCGACAACTTTTCCGGAGCTGGGTTTCAATCCAAGAACAAGTACCTGTTTGGAAAAGTGAGCCTTCAGATTAAGCTCATCGAGGGCGACTCTGCTGGAACCGTCACTGCTTTCTATATGTCATCGGACGGTCCACAACACAATGAGTTCGACTTTGAGTTTCTGGGGAACACGACAGGGGAACCTTATTCGGTGCAGACCAATTTATACGTGAATGGCGTGGGAAACAGAGAACAAAGATTGAACCTTTGGTTCGATCCCACCACGGAATTCCATTCCTACTCCATCTTCTGGAATCAGCGCCAAGTAGTGTTCCTAGTGGATGAGACGCCCATTAGAGTCCACACCAACATGGAAAACAAAGGGGTGCCCTTTCCCAAGGACCAAGCCATGGGTGTGTACAGTTCAATTTGGAATGCAGATGACTGGGCCACACAGGGTGGCAGGGTCAAGACAGATTGGTCCCATGCACCCTTCATTGCAACCTACAAGGGCTTTGAAATCAATGCCTGTGAGTACCCAGTTTCCATTGCAGCTGCAGATAAGGCAAAGAAGTGCATCAGCAATGGTGGCCAGAAGTACTGGTGGGATGAGCCTACTTTGTCAGAGCTAAGTGTCCACCAGAACCACCAGCTCGTTTGGGTGAAGGCTCACCACATGGTCTACGACTACTGCACCGATTCCGCTAGGTTTCCGGTGACTCCACTCGAGTGCGTGCATCACCGCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016740 GO:0016757 GO:0016758 GO:0016762 GO:0046527
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

297

Amino Acids

33.57

Weight (kDa)

5.67

Isoelectric Point (pI)

32.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03210 AT4G03210
fragaria_vesca FvH4_6g43860 FvH4_6g43860
malus_domestica MD09G1102600.v1.1 MD17G1089900.v1.1
prunus_persica Prupe.3G223200_v2.0.a1
pyrus_communis pycom09g02590 pycom17g08700
rosa_chinensis RchiOBHm_Chr2g0160611
rosa_laevigata RLG00000021226
rosa_multiflora Rmu_co8450919.1_g000001
rosa_roxburghii Rroxscaffold_2G00089560
rosa_rugosa Rorug02G0484200
rosa_samantha Rh2DG572700
rosa_wichuraiana Rw2G045500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 619
AccB1I GGYRCC 1 cut(s) 526
AccB7I CCANNNNNTGG 2 cut(s) 450, 550
AccI GTMKAC 1 cut(s) 822
AccIII TCCGGA 1 cut(s) 173
AciI CCGC 2 cut(s) 843, 883
AclWI GGATC 1 cut(s) 410
AcoI YGGCCR 1 cut(s) 730
AcsI RAATTY 3 cut(s) 86, 147, 428
AcuI CTGAAG 1 cut(s) 209
AcyI GRCGYC 1 cut(s) 488
AdeI CACNNNGTG 1 cut(s) 599
AfaI GTAC 4 cut(s) 203, 560, 677, 740
AfiI CCNNNNNNNGG 3 cut(s) 180, 450, 550
AgsI TTSAA 4 cut(s) 188, 403, 567, 659
AjnI CCWGG 1 cut(s) 110
AleI CACNNNNGTG 1 cut(s) 868
AluBI AGCT 8 cut(s) 14, 78, 97, 179, 235, 695, 769, 793
AluI AGCT 8 cut(s) 14, 78, 97, 179, 235, 695, 769, 793
Alw26I GTCTC 1 cut(s) 479
AlwI GGATC 1 cut(s) 410
Ama87I CYCGRG 1 cut(s) 866
Aor13HI TCCGGA 1 cut(s) 173
AoxI GGCC 2 cut(s) 588, 730
ApeKI GCWGC 2 cut(s) 692, 695
ApoI RAATTY 3 cut(s) 86, 147, 428
AspLEI GCGC 1 cut(s) 462
AspS9I GGNCC 4 cut(s) 290, 541, 588, 621
AsuHPI GGTGA 5 cut(s) 121, 803, 814, 868, 872
AvaI CYCGRG 1 cut(s) 866
AvaII GGWCC 3 cut(s) 290, 541, 621
BaeGI GKGCMC 1 cut(s) 531
BalI TGGCCA 1 cut(s) 732
BanI GGYRCC 1 cut(s) 526
BbvI GCAGC 2 cut(s) 682, 704
BccI CCATC 1 cut(s) 452
BciT130I CCWGG 1 cut(s) 112
BcoDI GTCTC 1 cut(s) 479
BfaI CTAG 3 cut(s) 476, 846, 889
BfmI CTRYAG 1 cut(s) 696
BfoI RGCGCY 1 cut(s) 463
BisI GCNGC 2 cut(s) 693, 696
BlpI GCTNAGC 1 cut(s) 79
BlsI GCNGC 2 cut(s) 694, 697
BmcAI AGTACT 1 cut(s) 740
Bme1390I CCNGG 1 cut(s) 112
Bme18I GGWCC 3 cut(s) 290, 541, 621
BmeT110I CYCGRG 1 cut(s) 866
BmgT120I GGNCC 4 cut(s) 290, 541, 588, 621
BmiI GGNNCC 4 cut(s) 259, 342, 528, 623
BmrFI CCNGG 1 cut(s) 112
BmrI ACTGGG 2 cut(s) 595, 674
BmsI GCATC 2 cut(s) 726, 886
BmuI ACTGGG 2 cut(s) 595, 674
BplI GAGNNNNNCTC 2 cut(s) 233, 265
Bpu1102I GCTNAGC 1 cut(s) 79
BsaAI YACGTR 1 cut(s) 370
BsaHI GRCGYC 1 cut(s) 488
BsaJI CCNNGG 4 cut(s) 111, 423, 537, 549
BsaWI WCCGGW 2 cut(s) 173, 853
Bsc4I CCNNNNNNNGG 3 cut(s) 180, 450, 550
Bse1I ACTGG 3 cut(s) 590, 680, 746
Bse3DI GCAATG 4 cut(s) 59, 636, 687, 730
BseAI TCCGGA 1 cut(s) 173
BseBI CCWGG 1 cut(s) 112
BseDI CCNNGG 4 cut(s) 111, 423, 537, 549
BseGI GGATG 2 cut(s) 487, 754
BseLI CCNNNNNNNGG 3 cut(s) 180, 450, 550
BseMI GCAATG 4 cut(s) 59, 636, 687, 730
BseMII CTCAG 3 cut(s) 29, 41, 93
BseNI ACTGG 3 cut(s) 590, 680, 746
BseSI GKGCMC 1 cut(s) 531
BseXI GCAGC 2 cut(s) 682, 704
BseYI CCCAGC 2 cut(s) 64, 179
BsgI GTGCAG 2 cut(s) 374, 817
BshFI GGCC 2 cut(s) 590, 732
BshNI GGYRCC 1 cut(s) 526
BsiHKCI CYCGRG 1 cut(s) 866
BsiSI CCGG 2 cut(s) 174, 854
BslFI GGGAC 1 cut(s) 607
BslI CCNNNNNNNGG 3 cut(s) 180, 450, 550
BsmAI GTCTC 1 cut(s) 479
BsmBI CGTCTC 1 cut(s) 479
BsmFI GGGAC 1 cut(s) 607
BsmI GAATGC 1 cut(s) 580
BsnI GGCC 2 cut(s) 590, 732
BsoBI CYCGRG 1 cut(s) 866
Bsp1286I GDGCHC 1 cut(s) 531
Bsp13I TCCGGA 1 cut(s) 173
Bsp1407I TGTACA 1 cut(s) 558
Bsp143I GATC 2 cut(s) 121, 415
Bsp1720I GCTNAGC 1 cut(s) 79
Bsp19I CCATGG 1 cut(s) 549
BspACI CCGC 2 cut(s) 843, 883
BspANI GGCC 2 cut(s) 590, 732
BspCNI CTCAG 3 cut(s) 30, 42, 92
BspEI TCCGGA 1 cut(s) 173
BspLI GGNNCC 4 cut(s) 259, 342, 528, 623
BspMAI CTGCAG 1 cut(s) 700
BspPI GGATC 1 cut(s) 410
BspT107I GGYRCC 1 cut(s) 526
BsrDI GCAATG 4 cut(s) 59, 636, 687, 730
BsrGI TGTACA 1 cut(s) 558
BsrI ACTGG 3 cut(s) 590, 680, 746
BssECI CCNNGG 4 cut(s) 111, 423, 537, 549
BssMI GATC 2 cut(s) 121, 415
BssNI GRCGYC 1 cut(s) 488
BssT1I CCWWGG 2 cut(s) 537, 549
Bst2UI CCWGG 1 cut(s) 112
Bst4CI ACNGT 3 cut(s) 262, 290, 563
BstACI GRCGYC 1 cut(s) 488
BstAUI TGTACA 1 cut(s) 558
BstBAI YACGTR 1 cut(s) 370
BstC8I GCNNGC 1 cut(s) 875
BstDEI CTNAG 4 cut(s) 38, 50, 79, 770
BstDSI CCRYGG 2 cut(s) 423, 549
BstF5I GGATG 2 cut(s) 487, 754
BstH2I RGCGCY 1 cut(s) 463
BstHHI GCGC 1 cut(s) 462
BstKTI GATC 2 cut(s) 124, 418
BstMAI GTCTC 1 cut(s) 479
BstMBI GATC 2 cut(s) 121, 415
BstMWI GCNNNNNNNGC 4 cut(s) 11, 103, 704, 729
BstNI CCWGG 1 cut(s) 112
BstSCI CCNGG 1 cut(s) 110
BstSFI CTRYAG 1 cut(s) 696
BstSLI GKGCMC 1 cut(s) 531
BstV1I GCAGC 2 cut(s) 682, 704
BsuRI GGCC 2 cut(s) 590, 732
BtgI CCRYGG 2 cut(s) 423, 549
BtsCI GGATG 2 cut(s) 487, 754
BtsI GCAGTG 1 cut(s) 264
BtsIMutI CAGTG 1 cut(s) 264
Cac8I GCNNGC 1 cut(s) 875
CfoI GCGC 1 cut(s) 462
Cfr13I GGNCC 4 cut(s) 290, 541, 588, 621
CseI GACGC 1 cut(s) 496
Csp6I GTAC 4 cut(s) 202, 559, 676, 739
CviAII CATG 5 cut(s) 154, 511, 550, 626, 817
CviQI GTAC 4 cut(s) 202, 559, 676, 739
DdeI CTNAG 4 cut(s) 38, 50, 79, 770
DpnI GATC 2 cut(s) 123, 417
DpnII GATC 2 cut(s) 121, 415
DraIII CACNNNGTG 1 cut(s) 599
DrdI GACNNNNNNGTC 1 cut(s) 619
DseDI GACNNNNNNGTC 1 cut(s) 619
EaeI YGGCCR 1 cut(s) 730
Eco130I CCWWGG 2 cut(s) 537, 549
Eco47I GGWCC 3 cut(s) 290, 541, 621
Eco57I CTGAAG 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 866
EcoRI GAATTC 1 cut(s) 428
EcoRII CCWGG 1 cut(s) 110
EcoT14I CCWWGG 2 cut(s) 537, 549
ErhI CCWWGG 2 cut(s) 537, 549
Esp3I CGTCTC 1 cut(s) 479
FaeI CATG 5 cut(s) 157, 514, 553, 629, 820
FaiI YATR 8 cut(s) 155, 276, 278, 367, 512, 551, 627, 818
FalI AAGNNNNNCTT 2 cut(s) 207, 239
FaqI GGGAC 1 cut(s) 607
FatI CATG 5 cut(s) 153, 510, 549, 625, 816
FblI GTMKAC 1 cut(s) 822
Fnu4HI GCNGC 2 cut(s) 693, 696
FokI GGATG 2 cut(s) 494, 761
Fsp4HI GCNGC 2 cut(s) 693, 696
FspBI CTAG 3 cut(s) 476, 846, 889
GlaI GCGC 1 cut(s) 461
GluI GCNGC 2 cut(s) 693, 696
GsaI CCCAGC 2 cut(s) 68, 183
HaeII RGCGCY 1 cut(s) 463
HaeIII GGCC 2 cut(s) 590, 732
HapII CCGG 2 cut(s) 174, 854
HgaI GACGC 1 cut(s) 496
HhaI GCGC 1 cut(s) 462
Hin1I GRCGYC 1 cut(s) 488
Hin1II CATG 5 cut(s) 157, 514, 553, 629, 820
Hin6I GCGC 1 cut(s) 460
HinP1I GCGC 1 cut(s) 460
HinfI GANTC 6 cut(s) 52, 248, 454, 498, 839, 859
HpaII CCGG 2 cut(s) 174, 854
HphI GGTGA 5 cut(s) 121, 803, 814, 868, 872
Hpy166II GTNNAC 5 cut(s) 293, 502, 559, 778, 823
Hpy188I TCNGA 5 cut(s) 39, 51, 228, 286, 766
Hpy188III TCNNGA 3 cut(s) 174, 451, 610
Hpy8I GTNNAC 5 cut(s) 293, 502, 559, 778, 823
HpyAV CCTTC 3 cut(s) 233, 643, 799
HpyCH4III ACNGT 3 cut(s) 262, 290, 563
HpyCH4IV ACGT 1 cut(s) 369
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 103, 704, 729
HpyF3I CTNAG 4 cut(s) 38, 50, 79, 770
HpySE526I ACGT 1 cut(s) 369
Hsp92I GRCGYC 1 cut(s) 488
Hsp92II CATG 5 cut(s) 157, 514, 553, 629, 820
HspAI GCGC 1 cut(s) 460
Kpn2I TCCGGA 1 cut(s) 173
Kzo9I GATC 2 cut(s) 121, 415
LmnI GCTCC 1 cut(s) 176
Lsp1109I GCAGC 2 cut(s) 682, 704
LweI GCATC 2 cut(s) 726, 886
MaeI CTAG 3 cut(s) 476, 846, 889
MaeII ACGT 1 cut(s) 369
MaeIII GTNAC 2 cut(s) 262, 856
MalI GATC 2 cut(s) 123, 417
MboI GATC 2 cut(s) 121, 415
MboII GAAGA 1 cut(s) 439
MhlI GDGCHC 1 cut(s) 531
MlsI TGGCCA 1 cut(s) 732
MluCI AATT 5 cut(s) 86, 147, 361, 428, 567
MluNI TGGCCA 1 cut(s) 732
MlyI GAGTC 4 cut(s) 61, 242, 507, 853
MnlI CCTC 2 cut(s) 130, 235
Mox20I TGGCCA 1 cut(s) 732
MroI TCCGGA 1 cut(s) 173
MscI TGGCCA 1 cut(s) 732
MseI TTAA 1 cut(s) 231
MslI CAYNNNNRTG 3 cut(s) 509, 554, 868
Msp20I TGGCCA 1 cut(s) 732
MspA1I CMGCKG 1 cut(s) 695
MspI CCGG 2 cut(s) 174, 854
MspR9I CCNGG 1 cut(s) 112
Mva1269I GAATGC 1 cut(s) 580
MvaI CCWGG 1 cut(s) 112
MwoI GCNNNNNNNGC 4 cut(s) 11, 103, 704, 729
NcoI CCATGG 1 cut(s) 549
NdeII GATC 2 cut(s) 121, 415
NlaIII CATG 5 cut(s) 157, 514, 553, 629, 820
NlaIV GGNNCC 4 cut(s) 259, 342, 528, 623
NmuCI GTSAC 2 cut(s) 262, 856
OliI CACNNNNGTG 1 cut(s) 868
PaeR7I CTCGAG 1 cut(s) 866
PcsI WCGNNNNNNNCGW 1 cut(s) 375
PctI GAATGC 1 cut(s) 580
PfeI GAWTC 2 cut(s) 454, 839
PflMI CCANNNNNTGG 2 cut(s) 450, 550
PkrI GCNGC 2 cut(s) 694, 697
PleI GAGTC 4 cut(s) 60, 242, 506, 853
PpsI GAGTC 4 cut(s) 60, 242, 506, 853
Ppu21I YACGTR 1 cut(s) 370
Psp6I CCWGG 1 cut(s) 110
PspFI CCCAGC 2 cut(s) 64, 179
PspGI CCWGG 1 cut(s) 110
PspN4I GGNNCC 4 cut(s) 259, 342, 528, 623
PspPI GGNCC 4 cut(s) 290, 541, 588, 621
PspXI VCTCGAGB 1 cut(s) 866
PstI CTGCAG 1 cut(s) 700
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 4 cut(s) 203, 560, 677, 740
RsaNI GTAC 4 cut(s) 202, 559, 676, 739
RseI CAYNNNNRTG 3 cut(s) 509, 554, 868
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 2 cut(s) 693, 696
Sau3AI GATC 2 cut(s) 121, 415
Sau96I GGNCC 4 cut(s) 290, 541, 588, 621
ScaI AGTACT 1 cut(s) 740
SchI GAGTC 4 cut(s) 61, 242, 507, 853
ScrFI CCNGG 1 cut(s) 112
SduI GDGCHC 1 cut(s) 531
SfaNI GCATC 2 cut(s) 726, 886
SfcI CTRYAG 1 cut(s) 696
Sfr274I CTCGAG 1 cut(s) 866
SinI GGWCC 3 cut(s) 290, 541, 621
SlaI CTCGAG 1 cut(s) 866
SmiMI CAYNNNNRTG 3 cut(s) 509, 554, 868
SmlI CTYRAG 1 cut(s) 866
SmoI CTYRAG 1 cut(s) 866
Sse9I AATT 5 cut(s) 86, 147, 361, 428, 567
SsiI CCGC 2 cut(s) 843, 883
SspMI CTAG 3 cut(s) 476, 846, 889
StyD4I CCNGG 1 cut(s) 110
StyI CCWWGG 2 cut(s) 537, 549
TaaI ACNGT 3 cut(s) 262, 290, 563
TaiI ACGT 1 cut(s) 372
TaqI TCGA 6 cut(s) 120, 162, 240, 309, 414, 867
TasI AATT 5 cut(s) 86, 147, 361, 428, 567
TatI WGTACW 2 cut(s) 558, 738
TfiI GAWTC 2 cut(s) 454, 839
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 1 cut(s) 271
TseFI GTSAC 2 cut(s) 262, 856
TseI GCWGC 2 cut(s) 692, 695
Tsp45I GTSAC 2 cut(s) 262, 856
TspDTI ATGAA 2 cut(s) 170, 625
TspGWI ACGGA 1 cut(s) 440
TspRI CASTG 1 cut(s) 271
Van91I CCANNNNNTGG 2 cut(s) 450, 550
VpaK11BI GGWCC 3 cut(s) 290, 541, 621
XapI RAATTY 3 cut(s) 86, 147, 428
XhoI CTCGAG 1 cut(s) 866
XmiI GTMKAC 1 cut(s) 822
XspI CTAG 3 cut(s) 476, 846, 889
ZrmI AGTACT 1 cut(s) 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.