pycom17g08700

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0015387
Physical Location & Seq
Reverse (-)
6526147 .. 6528425
2279 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g08700.1

Sequence Viewer

Length: 867 bp
ATGGCTTTTTCTCTGAGTGTTTTTGTGAGTCTTGCATTGCTGGGTTTGGTCAGCTCAGTAAAATTTGATGACCTTTTCCAGCCAACCTGGGCTCTCGATCACTTCACCTATGAGGGAGAGCAAGTTCACATGAAACTCGACAACTTTTCCGGAGCTGGGTTTCAATCCAAGAACAAGTACTTGTTTGGAAAAGTGAGCATTCAGATTAAGCTCATCGAGGGTGACTCTGCCGGAACTGTCACTGCTTTCTATATGTCATCGGACGGTCCACAACACGATGAGTTCGACTTCGAGTTTCTGGGGAACACTACAGGGGAACCTTATTCAGTGCAGACCAACATATATGTCAATGGTGTGGGAAACAGAGAGCAAAGGTTGAACCTTTGGTTCGATCCCACCACGGAATTCCACTCCTACTCCATCTTCTGGAATCAGCGCCAAGTAGTGTTCCTAGTGGATGAGACGCCCATTAGAGTCCACACCAACATGGAGAACAAGGGGGTGCCCTTCCCCAAGGACCAAGCCATGGGTGTCTACAGTTCAATTTGGAATGCAGATGACTGGGCCACACAGGGTGGTAGGGTCAAGACAGATTGGTCCCATGCACCCTTCGTCGCAACCTACAAGGGCTTTGACATCAATGCCTGTGAGTGCCCAGTTTCTTTTGCAGCCGCAGATAAGGCAAAGAAGTGCAGCAGCAGTGGTGACCAGAAGTACTGGTGGGATGAGCCTACTTTGTCCGAGCTCAATCTCCACCAGAACCACCAGCTCGTGTGGGTTAAGGCTCACCACATGGTCTATGACTACTGCACCGATTCCGCTAGGTTTCCGGTGACTCCACTTGAGTGCGTGCACCACCGCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016740 GO:0016757 GO:0016758 GO:0016762 GO:0046527
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

289

Amino Acids

32.81

Weight (kDa)

5.46

Isoelectric Point (pI)

34.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 24 - 200 4.3e-63 Glycosyl hydrolases family 16
XET_C PF06955 237 - 283 6.4e-17 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03210 AT4G03210
fragaria_vesca FvH4_6g43860 FvH4_6g43860
malus_domestica MD09G1102600.v1.1 MD17G1089900.v1.1
prunus_persica Prupe.3G223200_v2.0.a1
pyrus_communis pycom09g02590 pycom17g08700
rosa_chinensis RchiOBHm_Chr2g0160611
rosa_laevigata RLG00000021226
rosa_multiflora Rmu_co8450919.1_g000001
rosa_roxburghii Rroxscaffold_2G00089560
rosa_rugosa Rorug02G0484200
rosa_samantha Rh2DG572700
rosa_wichuraiana Rw2G045500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 595
AccB1I GGYRCC 1 cut(s) 502
AccB7I CCANNNNNTGG 2 cut(s) 426, 526
AccI GTMKAC 1 cut(s) 534
AccIII TCCGGA 1 cut(s) 149
AciI CCGC 3 cut(s) 672, 819, 859
AclWI GGATC 1 cut(s) 386
AcsI RAATTY 2 cut(s) 62, 404
AcyI GRCGYC 1 cut(s) 464
AdeI CACNNNGTG 1 cut(s) 575
AfaI GTAC 2 cut(s) 179, 716
AfiI CCNNNNNNNGG 3 cut(s) 156, 426, 526
AgsI TTSAA 3 cut(s) 164, 379, 543
AjnI CCWGG 1 cut(s) 86
AleI CACNNNNGTG 1 cut(s) 844
AloI GAACNNNNNNTCC 2 cut(s) 108, 140
AluBI AGCT 5 cut(s) 54, 155, 211, 745, 769
AluI AGCT 5 cut(s) 54, 155, 211, 745, 769
Alw21I GWGCWC 2 cut(s) 747, 855
Alw26I GTCTC 1 cut(s) 455
Alw44I GTGCAC 1 cut(s) 851
AlwI GGATC 1 cut(s) 386
Aor13HI TCCGGA 1 cut(s) 149
AoxI GGCC 1 cut(s) 564
ApaLI GTGCAC 1 cut(s) 851
ApeKI GCWGC 3 cut(s) 668, 693, 696
ApoI RAATTY 2 cut(s) 62, 404
AspLEI GCGC 1 cut(s) 438
AspS9I GGNCC 4 cut(s) 266, 517, 564, 597
AsuHPI GGTGA 5 cut(s) 97, 233, 716, 779, 844
AvaII GGWCC 3 cut(s) 266, 517, 597
BaeGI GKGCMC 3 cut(s) 507, 656, 855
BanI GGYRCC 1 cut(s) 502
BanII GRGCYC 2 cut(s) 94, 747
BauI CACGAG 1 cut(s) 770
Bbv12I GWGCWC 2 cut(s) 747, 855
BbvI GCAGC 3 cut(s) 680, 705, 708
BccI CCATC 1 cut(s) 428
BciT130I CCWGG 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 455
BfaI CTAG 3 cut(s) 452, 822, 865
BfmI CTRYAG 2 cut(s) 309, 535
BfoI RGCGCY 1 cut(s) 439
BisI GCNGC 4 cut(s) 669, 672, 694, 697
BlsI GCNGC 4 cut(s) 670, 673, 695, 698
BmcAI AGTACT 2 cut(s) 179, 716
Bme1390I CCNGG 1 cut(s) 88
Bme18I GGWCC 3 cut(s) 266, 517, 597
BmgT120I GGNCC 4 cut(s) 266, 517, 564, 597
BmiI GGNNCC 3 cut(s) 318, 504, 599
BmrFI CCNGG 1 cut(s) 88
BmrI ACTGGG 2 cut(s) 571, 650
BmuI ACTGGG 2 cut(s) 571, 650
BplI GAGNNNNNCTC 2 cut(s) 209, 241
BpuEI CTTGAG 1 cut(s) 863
BsaHI GRCGYC 1 cut(s) 464
BsaJI CCNNGG 4 cut(s) 87, 399, 513, 525
BsaWI WCCGGW 2 cut(s) 149, 829
BsaXI ACNNNNNCTCC 4 cut(s) 108, 138, 401, 431
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 426, 526
Bse1I ACTGG 3 cut(s) 566, 656, 722
Bse3DI GCAATG 1 cut(s) 35
BseAI TCCGGA 1 cut(s) 149
BseBI CCWGG 1 cut(s) 88
BseDI CCNNGG 4 cut(s) 87, 399, 513, 525
BseGI GGATG 2 cut(s) 463, 730
BseLI CCNNNNNNNGG 3 cut(s) 156, 426, 526
BseMI GCAATG 1 cut(s) 35
BseMII CTCAG 2 cut(s) 5, 69
BseNI ACTGG 3 cut(s) 566, 656, 722
BseSI GKGCMC 3 cut(s) 507, 656, 855
BseXI GCAGC 3 cut(s) 680, 705, 708
BseYI CCCAGC 2 cut(s) 40, 155
BsgI GTGCAG 3 cut(s) 350, 712, 793
BshFI GGCC 1 cut(s) 566
BshNI GGYRCC 1 cut(s) 502
BsiHKAI GWGCWC 2 cut(s) 747, 855
BsiSI CCGG 3 cut(s) 150, 231, 830
BslFI GGGAC 1 cut(s) 583
BslI CCNNNNNNNGG 3 cut(s) 156, 426, 526
BsmAI GTCTC 1 cut(s) 455
BsmBI CGTCTC 1 cut(s) 455
BsmFI GGGAC 1 cut(s) 583
BsmI GAATGC 2 cut(s) 198, 556
BsnI GGCC 1 cut(s) 566
Bsp1286I GDGCHC 5 cut(s) 94, 507, 656, 747, 855
Bsp13I TCCGGA 1 cut(s) 149
Bsp143I GATC 2 cut(s) 97, 391
Bsp19I CCATGG 1 cut(s) 525
BspACI CCGC 3 cut(s) 672, 819, 859
BspANI GGCC 1 cut(s) 566
BspCNI CTCAG 2 cut(s) 6, 68
BspEI TCCGGA 1 cut(s) 149
BspLI GGNNCC 3 cut(s) 318, 504, 599
BspPI GGATC 1 cut(s) 386
BspT107I GGYRCC 1 cut(s) 502
BsrDI GCAATG 1 cut(s) 35
BsrI ACTGG 3 cut(s) 566, 656, 722
BssECI CCNNGG 4 cut(s) 87, 399, 513, 525
BssMI GATC 2 cut(s) 97, 391
BssNI GRCGYC 1 cut(s) 464
BssSI CACGAG 1 cut(s) 770
BssT1I CCWWGG 2 cut(s) 513, 525
Bst2BI CACGAG 1 cut(s) 770
Bst2UI CCWGG 1 cut(s) 88
Bst4CI ACNGT 3 cut(s) 238, 266, 539
BstACI GRCGYC 1 cut(s) 464
BstC8I GCNNGC 1 cut(s) 851
BstDEI CTNAG 2 cut(s) 14, 55
BstDSI CCRYGG 2 cut(s) 399, 525
BstEII GGTNACC 1 cut(s) 704
BstF5I GGATG 2 cut(s) 463, 730
BstH2I RGCGCY 1 cut(s) 439
BstHHI GCGC 1 cut(s) 438
BstKTI GATC 2 cut(s) 100, 394
BstMAI GTCTC 1 cut(s) 455
BstMBI GATC 2 cut(s) 97, 391
BstMWI GCNNNNNNNGC 1 cut(s) 680
BstNI CCWGG 1 cut(s) 88
BstPI GGTNACC 1 cut(s) 704
BstSCI CCNGG 1 cut(s) 86
BstSFI CTRYAG 2 cut(s) 309, 535
BstSLI GKGCMC 3 cut(s) 507, 656, 855
BstV1I GCAGC 3 cut(s) 680, 705, 708
BsuRI GGCC 1 cut(s) 566
BtgI CCRYGG 2 cut(s) 399, 525
BtsCI GGATG 2 cut(s) 463, 730
BtsI GCAGTG 2 cut(s) 240, 706
BtsIMutI CAGTG 3 cut(s) 240, 333, 706
Cac8I GCNNGC 1 cut(s) 851
CfoI GCGC 1 cut(s) 438
Cfr13I GGNCC 4 cut(s) 266, 517, 564, 597
CseI GACGC 1 cut(s) 472
Csp6I GTAC 2 cut(s) 178, 715
CviAII CATG 5 cut(s) 130, 487, 526, 602, 793
CviQI GTAC 2 cut(s) 178, 715
DdeI CTNAG 2 cut(s) 14, 55
DpnI GATC 2 cut(s) 99, 393
DpnII GATC 2 cut(s) 97, 391
DraIII CACNNNGTG 1 cut(s) 575
DrdI GACNNNNNNGTC 1 cut(s) 595
DseDI GACNNNNNNGTC 1 cut(s) 595
Ecl136II GAGCTC 1 cut(s) 745
Eco130I CCWWGG 2 cut(s) 513, 525
Eco24I GRGCYC 2 cut(s) 94, 747
Eco47I GGWCC 3 cut(s) 266, 517, 597
Eco53kI GAGCTC 1 cut(s) 745
Eco91I GGTNACC 1 cut(s) 704
EcoICRI GAGCTC 1 cut(s) 745
EcoO65I GGTNACC 1 cut(s) 704
EcoRI GAATTC 1 cut(s) 404
EcoRII CCWGG 1 cut(s) 86
EcoT14I CCWWGG 2 cut(s) 513, 525
EcoT38I GRGCYC 2 cut(s) 94, 747
ErhI CCWWGG 2 cut(s) 513, 525
Esp3I CGTCTC 1 cut(s) 455
FaeI CATG 5 cut(s) 133, 490, 529, 605, 796
FaqI GGGAC 1 cut(s) 583
FatI CATG 5 cut(s) 129, 486, 525, 601, 792
FblI GTMKAC 1 cut(s) 534
Fnu4HI GCNGC 4 cut(s) 669, 672, 694, 697
FokI GGATG 2 cut(s) 470, 737
FriOI GRGCYC 2 cut(s) 94, 747
Fsp4HI GCNGC 4 cut(s) 669, 672, 694, 697
FspBI CTAG 3 cut(s) 452, 822, 865
GlaI GCGC 1 cut(s) 437
GluI GCNGC 4 cut(s) 669, 672, 694, 697
GsaI CCCAGC 2 cut(s) 44, 159
HaeII RGCGCY 1 cut(s) 439
HaeIII GGCC 1 cut(s) 566
HapII CCGG 3 cut(s) 150, 231, 830
HgaI GACGC 1 cut(s) 472
HhaI GCGC 1 cut(s) 438
Hin1I GRCGYC 1 cut(s) 464
Hin1II CATG 5 cut(s) 133, 490, 529, 605, 796
Hin6I GCGC 1 cut(s) 436
HinP1I GCGC 1 cut(s) 436
HinfI GANTC 6 cut(s) 28, 224, 430, 474, 815, 835
HpaII CCGG 3 cut(s) 150, 231, 830
HphI GGTGA 5 cut(s) 97, 233, 716, 779, 844
Hpy166II GTNNAC 5 cut(s) 127, 269, 478, 535, 853
Hpy188I TCNGA 4 cut(s) 15, 204, 262, 742
Hpy188III TCNNGA 4 cut(s) 95, 150, 427, 586
Hpy8I GTNNAC 5 cut(s) 127, 269, 478, 535, 853
Hpy99I CGWCG 1 cut(s) 617
HpyAV CCTTC 2 cut(s) 517, 619
HpyCH4III ACNGT 3 cut(s) 238, 266, 539
HpyCH4V TGCA 8 cut(s) 35, 331, 554, 605, 668, 693, 810, 853
HpyF10VI GCNNNNNNNGC 1 cut(s) 680
HpyF3I CTNAG 2 cut(s) 14, 55
Hsp92I GRCGYC 1 cut(s) 464
Hsp92II CATG 5 cut(s) 133, 490, 529, 605, 796
HspAI GCGC 1 cut(s) 436
Kpn2I TCCGGA 1 cut(s) 149
Kzo9I GATC 2 cut(s) 97, 391
LmnI GCTCC 1 cut(s) 152
Lsp1109I GCAGC 3 cut(s) 680, 705, 708
MaeI CTAG 3 cut(s) 452, 822, 865
MaeIII GTNAC 4 cut(s) 221, 238, 704, 832
MalI GATC 2 cut(s) 99, 393
MboI GATC 2 cut(s) 97, 391
MboII GAAGA 1 cut(s) 415
MhlI GDGCHC 5 cut(s) 94, 507, 656, 747, 855
MluCI AATT 3 cut(s) 62, 404, 543
MlyI GAGTC 4 cut(s) 37, 218, 483, 829
MnlI CCTC 2 cut(s) 106, 211
MroI TCCGGA 1 cut(s) 149
MseI TTAA 2 cut(s) 207, 780
MslI CAYNNNNRTG 2 cut(s) 485, 844
MspI CCGG 3 cut(s) 150, 231, 830
MspR9I CCNGG 1 cut(s) 88
Mva1269I GAATGC 2 cut(s) 198, 556
MvaI CCWGG 1 cut(s) 88
MwoI GCNNNNNNNGC 1 cut(s) 680
NcoI CCATGG 1 cut(s) 525
NdeII GATC 2 cut(s) 97, 391
NlaIII CATG 5 cut(s) 133, 490, 529, 605, 796
NlaIV GGNNCC 3 cut(s) 318, 504, 599
NmuCI GTSAC 4 cut(s) 221, 238, 704, 832
OliI CACNNNNGTG 1 cut(s) 844
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PctI GAATGC 2 cut(s) 198, 556
PfeI GAWTC 2 cut(s) 430, 815
PflMI CCANNNNNTGG 2 cut(s) 426, 526
PkrI GCNGC 4 cut(s) 670, 673, 695, 698
PleI GAGTC 4 cut(s) 36, 218, 482, 829
PpsI GAGTC 4 cut(s) 36, 218, 482, 829
Psp124BI GAGCTC 1 cut(s) 747
Psp6I CCWGG 1 cut(s) 86
PspEI GGTNACC 1 cut(s) 704
PspFI CCCAGC 2 cut(s) 40, 155
PspGI CCWGG 1 cut(s) 86
PspN4I GGNNCC 3 cut(s) 318, 504, 599
PspPI GGNCC 4 cut(s) 266, 517, 564, 597
RsaI GTAC 2 cut(s) 179, 716
RsaNI GTAC 2 cut(s) 178, 715
RseI CAYNNNNRTG 2 cut(s) 485, 844
SacI GAGCTC 1 cut(s) 747
SaqAI TTAA 2 cut(s) 207, 780
SatI GCNGC 4 cut(s) 669, 672, 694, 697
Sau3AI GATC 2 cut(s) 97, 391
Sau96I GGNCC 4 cut(s) 266, 517, 564, 597
ScaI AGTACT 2 cut(s) 179, 716
SchI GAGTC 4 cut(s) 37, 218, 483, 829
ScrFI CCNGG 1 cut(s) 88
SduI GDGCHC 5 cut(s) 94, 507, 656, 747, 855
SfcI CTRYAG 2 cut(s) 309, 535
SinI GGWCC 3 cut(s) 266, 517, 597
SmiMI CAYNNNNRTG 2 cut(s) 485, 844
SmlI CTYRAG 1 cut(s) 842
SmoI CTYRAG 1 cut(s) 842
Sse9I AATT 3 cut(s) 62, 404, 543
SsiI CCGC 3 cut(s) 672, 819, 859
SspMI CTAG 3 cut(s) 452, 822, 865
SstI GAGCTC 1 cut(s) 747
StyD4I CCNGG 1 cut(s) 86
StyI CCWWGG 2 cut(s) 513, 525
TaaI ACNGT 3 cut(s) 238, 266, 539
TaqI TCGA 6 cut(s) 96, 138, 216, 285, 291, 390
TasI AATT 3 cut(s) 62, 404, 543
TatI WGTACW 2 cut(s) 177, 714
TauI GCSGC 1 cut(s) 674
TfiI GAWTC 2 cut(s) 430, 815
Tru1I TTAA 2 cut(s) 207, 780
Tru9I TTAA 2 cut(s) 207, 780
TscAI CASTG 3 cut(s) 247, 333, 706
TseFI GTSAC 4 cut(s) 221, 238, 704, 832
TseI GCWGC 3 cut(s) 668, 693, 696
Tsp45I GTSAC 4 cut(s) 221, 238, 704, 832
TspDTI ATGAA 1 cut(s) 146
TspGWI ACGGA 1 cut(s) 416
TspRI CASTG 3 cut(s) 247, 333, 706
Van91I CCANNNNNTGG 2 cut(s) 426, 526
VneI GTGCAC 1 cut(s) 851
VpaK11BI GGWCC 3 cut(s) 266, 517, 597
XapI RAATTY 2 cut(s) 62, 404
XmiI GTMKAC 1 cut(s) 534
XspI CTAG 3 cut(s) 452, 822, 865
ZrmI AGTACT 2 cut(s) 179, 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.