MD17G1150200.v1.1

Auxin-repressed 12.5 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005839
Physical Location & Seq
Reverse (-)
13789622 .. 13791418
1797 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1150200.v1.1.491

Sequence Viewer

Length: 375 bp
ATGGGTTTCCTTCACAAGCTTTGGGATGAAACACTCGCTGGACCGGCACCCGAATCCGGCCTCGGCAAGCTCCGGAAGTACGACTCTATGTCTATCCCGTCCTCTCCGCCAATGGCTGCGGATGAGGTGCCGGTCACTCGCTCCATTACTATTCTCAGGACTAGTTCGTCCACTCTTGGAAACGTTTCACCTGATTCCGGCTCCCCCTCGGAGTCCCCGACCACACCAGGAACTCCGAGAACCCCTGGAACACCAGGTGCATTGAACTTCAAGAAATTAACAAGGAGGAAATCGTCTAATGATGCTTTGACACGCGCCGAACCCAGAAGTCCGACTGGTTACGATTGGATGGTAATTACTGCTTTGGATCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_repressed

Protein Analysis

125

Amino Acids

13.23

Weight (kDa)

9.52

Isoelectric Point (pI)

71.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_repressed PF05564 7 - 117 3.1e-09 Dormancy/auxin associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 166
AccB1I GGYRCC 2 cut(s) 46, 127
AccII CGCG 1 cut(s) 315
AccIII TCCGGA 1 cut(s) 72
AciI CCGC 2 cut(s) 107, 119
AclI AACGTT 1 cut(s) 183
AdeI CACNNNGTG 1 cut(s) 257
AfaI GTAC 1 cut(s) 80
AfiI CCNNNNNNNGG 2 cut(s) 56, 197
AgsI TTSAA 2 cut(s) 265, 271
AhdI GACNNNNNGTC 1 cut(s) 88
AhlI ACTAGT 1 cut(s) 161
AjnI CCWGG 3 cut(s) 226, 244, 253
AluBI AGCT 2 cut(s) 19, 70
AluI AGCT 2 cut(s) 19, 70
Aor13HI TCCGGA 1 cut(s) 72
AoxI GGCC 1 cut(s) 58
ApeKI GCWGC 1 cut(s) 116
Asp700I GAANNNNTTC 1 cut(s) 184
AspLEI GCGC 1 cut(s) 317
AspS9I GGNCC 1 cut(s) 41
AsuHPI GGTGA 1 cut(s) 180
AvaII GGWCC 1 cut(s) 41
BanI GGYRCC 2 cut(s) 46, 127
BbvI GCAGC 1 cut(s) 103
BccI CCATC 1 cut(s) 343
BciT130I CCWGG 3 cut(s) 228, 246, 255
BcuI ACTAGT 1 cut(s) 161
BfaI CTAG 1 cut(s) 162
BisI GCNGC 1 cut(s) 117
BlsI GCNGC 1 cut(s) 118
Bme1390I CCNGG 3 cut(s) 228, 246, 255
Bme18I GGWCC 1 cut(s) 41
BmeRI GACNNNNNGTC 1 cut(s) 88
BmgT120I GGNCC 1 cut(s) 41
BmiI GGNNCC 3 cut(s) 48, 129, 202
BmrFI CCNGG 3 cut(s) 228, 246, 255
BmsI GCATC 1 cut(s) 292
BsaJI CCNNGG 3 cut(s) 61, 207, 244
BsaWI WCCGGW 1 cut(s) 72
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 197
Bse118I RCCGGY 2 cut(s) 43, 130
Bse1I ACTGG 1 cut(s) 340
BseAI TCCGGA 1 cut(s) 72
BseBI CCWGG 3 cut(s) 228, 246, 255
BseDI CCNNGG 3 cut(s) 61, 207, 244
BseGI GGATG 3 cut(s) 31, 127, 354
BseLI CCNNNNNNNGG 2 cut(s) 56, 197
BseMII CTCAG 1 cut(s) 169
BseNI ACTGG 1 cut(s) 340
BseXI GCAGC 1 cut(s) 103
Bsh1236I CGCG 1 cut(s) 315
BshFI GGCC 1 cut(s) 60
BshNI GGYRCC 2 cut(s) 46, 127
BsiSI CCGG 5 cut(s) 44, 57, 73, 131, 198
BslFI GGGAC 1 cut(s) 199
BslI CCNNNNNNNGG 2 cut(s) 56, 197
BsmFI GGGAC 1 cut(s) 199
BsnI GGCC 1 cut(s) 60
Bsp13I TCCGGA 1 cut(s) 72
Bsp143I GATC 1 cut(s) 367
BspACI CCGC 2 cut(s) 107, 119
BspANI GGCC 1 cut(s) 60
BspCNI CTCAG 1 cut(s) 168
BspEI TCCGGA 1 cut(s) 72
BspFNI CGCG 1 cut(s) 315
BspLI GGNNCC 3 cut(s) 48, 129, 202
BspT107I GGYRCC 2 cut(s) 46, 127
BsrFI RCCGGY 2 cut(s) 43, 130
BsrI ACTGG 1 cut(s) 340
BssAI RCCGGY 2 cut(s) 43, 130
BssECI CCNNGG 3 cut(s) 61, 207, 244
BssMI GATC 1 cut(s) 367
Bst2UI CCWGG 3 cut(s) 228, 246, 255
BstC8I GCNNGC 1 cut(s) 68
BstDEI CTNAG 1 cut(s) 155
BstF5I GGATG 3 cut(s) 31, 127, 354
BstFNI CGCG 1 cut(s) 315
BstHHI GCGC 1 cut(s) 317
BstKTI GATC 1 cut(s) 370
BstMBI GATC 1 cut(s) 367
BstMWI GCNNNNNNNGC 1 cut(s) 44
BstNI CCWGG 3 cut(s) 228, 246, 255
BstSCI CCNGG 3 cut(s) 226, 244, 253
BstUI CGCG 1 cut(s) 315
BstV1I GCAGC 1 cut(s) 103
BsuRI GGCC 1 cut(s) 60
BtsCI GGATG 3 cut(s) 31, 127, 354
Cac8I GCNNGC 1 cut(s) 68
CfoI GCGC 1 cut(s) 317
Cfr10I RCCGGY 2 cut(s) 43, 130
Cfr13I GGNCC 1 cut(s) 41
CsiI ACCWGGT 1 cut(s) 253
Csp6I GTAC 1 cut(s) 79
CviJI RGCY 5 cut(s) 19, 60, 70, 116, 201
CviKI_1 RGCY 5 cut(s) 19, 60, 70, 116, 201
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 1 cut(s) 155
DpnI GATC 1 cut(s) 369
DpnII GATC 1 cut(s) 367
DraIII CACNNNGTG 1 cut(s) 257
DrdI GACNNNNNNGTC 1 cut(s) 166
DriI GACNNNNNGTC 1 cut(s) 88
DseDI GACNNNNNNGTC 1 cut(s) 166
Eam1105I GACNNNNNGTC 1 cut(s) 88
EciI GGCGGA 1 cut(s) 96
Eco47I GGWCC 1 cut(s) 41
EcoRII CCWGG 3 cut(s) 226, 244, 253
FaiI YATR 1 cut(s) 89
FaqI GGGAC 1 cut(s) 199
Fnu4HI GCNGC 1 cut(s) 117
FokI GGATG 3 cut(s) 38, 134, 361
Fsp4HI GCNGC 1 cut(s) 117
FspBI CTAG 1 cut(s) 162
GlaI GCGC 1 cut(s) 316
GluI GCNGC 1 cut(s) 117
HaeIII GGCC 1 cut(s) 60
HapII CCGG 5 cut(s) 44, 57, 73, 131, 198
HhaI GCGC 1 cut(s) 317
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HindIII AAGCTT 1 cut(s) 17
HinfI GANTC 4 cut(s) 53, 83, 194, 212
HpaII CCGG 5 cut(s) 44, 57, 73, 131, 198
HphI GGTGA 1 cut(s) 180
Hpy166II GTNNAC 1 cut(s) 171
Hpy188I TCNGA 3 cut(s) 211, 237, 333
Hpy188III TCNNGA 3 cut(s) 73, 157, 271
Hpy8I GTNNAC 1 cut(s) 171
HpyAV CCTTC 1 cut(s) 20
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 1 cut(s) 260
HpyF10VI GCNNNNNNNGC 1 cut(s) 44
HpyF3I CTNAG 1 cut(s) 155
HpySE526I ACGT 1 cut(s) 183
HspAI GCGC 1 cut(s) 315
Kpn2I TCCGGA 1 cut(s) 72
Kzo9I GATC 1 cut(s) 367
LmnI GCTCC 3 cut(s) 75, 146, 206
Lsp1109I GCAGC 1 cut(s) 103
LweI GCATC 1 cut(s) 292
MabI ACCWGGT 1 cut(s) 253
MaeI CTAG 1 cut(s) 162
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 2 cut(s) 133, 338
MalI GATC 1 cut(s) 369
MboI GATC 1 cut(s) 367
MluCI AATT 2 cut(s) 275, 354
MlyI GAGTC 2 cut(s) 77, 221
MmeI TCCRAC 1 cut(s) 356
MnlI CCTC 5 cut(s) 71, 112, 118, 217, 279
MroI TCCGGA 1 cut(s) 72
MroXI GAANNNNTTC 1 cut(s) 184
MseI TTAA 1 cut(s) 278
MspI CCGG 5 cut(s) 44, 57, 73, 131, 198
MspR9I CCNGG 3 cut(s) 228, 246, 255
MvaI CCWGG 3 cut(s) 228, 246, 255
MvnI CGCG 1 cut(s) 315
MwoI GCNNNNNNNGC 1 cut(s) 44
NdeII GATC 1 cut(s) 367
NlaIV GGNNCC 3 cut(s) 48, 129, 202
NmeAIII GCCGAG 1 cut(s) 42
NmuCI GTSAC 1 cut(s) 133
PcsI WCGNNNNNNNCGW 1 cut(s) 215
PdmI GAANNNNTTC 1 cut(s) 184
PfeI GAWTC 2 cut(s) 53, 194
PkrI GCNGC 1 cut(s) 118
PleI GAGTC 2 cut(s) 77, 220
PpsI GAGTC 2 cut(s) 77, 220
Psp1406I AACGTT 1 cut(s) 183
Psp6I CCWGG 3 cut(s) 226, 244, 253
PspGI CCWGG 3 cut(s) 226, 244, 253
PspN4I GGNNCC 3 cut(s) 48, 129, 202
PspPI GGNCC 1 cut(s) 41
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
SaqAI TTAA 1 cut(s) 278
SatI GCNGC 1 cut(s) 117
Sau3AI GATC 1 cut(s) 367
Sau96I GGNCC 1 cut(s) 41
SchI GAGTC 2 cut(s) 77, 221
ScrFI CCNGG 3 cut(s) 228, 246, 255
SetI ASST 6 cut(s) 21, 72, 129, 186, 193, 259
SexAI ACCWGGT 1 cut(s) 253
SfaNI GCATC 1 cut(s) 292
SinI GGWCC 1 cut(s) 41
SpeI ACTAGT 1 cut(s) 161
Sse9I AATT 2 cut(s) 275, 354
SsiI CCGC 2 cut(s) 107, 119
SspMI CTAG 1 cut(s) 162
StyD4I CCNGG 3 cut(s) 226, 244, 253
TaiI ACGT 1 cut(s) 186
TasI AATT 2 cut(s) 275, 354
TfiI GAWTC 2 cut(s) 53, 194
Tru1I TTAA 1 cut(s) 278
Tru9I TTAA 1 cut(s) 278
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 1 cut(s) 116
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 1 cut(s) 42
VpaK11BI GGWCC 1 cut(s) 41
XmnI GAANNNNTTC 1 cut(s) 184
XspI CTAG 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.