Rorug02G0396700

Auxin-repressed 12.5 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005839
Physical Location & Seq
Reverse (-)
50716762 .. 50720764
4003 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0396700.1

Sequence Viewer

Length: 588 bp
ATGATGAAACTGCAGAGAAACGTTTCTTTCGTAGATTGCCTGACCCACGATGCGCTGATGGCAGCTATGCGCAATGGTGTCTCCATTATGGATGGAGCATTGCTTCTCGTAGCAGCTAATGAAGAACTGCCCCCAGCACAAACCAAAGAGCATTTGGCTACTCTTGCAATTATGCGTCTTAAAGACATTATTTTCCTCCAAAAGAAAGTAGATCTTGAAGTTCAAGATGAGGATCGGGCCATTGAGCAACAACAAAAAAAATGGAGGAGGAGTTCAACGAATCAGCCAACTGCTCAACCTGAGCACAACATGGATGTAGTTGATGAGCACATCGTCAAAAATATCCCAGTACCACAAAGGGACTTGGCCTCACCTCCTTATATTACAATTATTCATTCTTTTGACGTCAACAAATCTGGGTATGAGATTGATCGCTTGAGAGGCGGTGTGGTGGGTGGAACCAGGGTCACGGGTGTTGTGAAACTGAACCACTTGATTGAGGTTTGCCCGGGGAAGGTTGAAAAGGATGAGAACGACAACTTCAGATGCACGCCATTATATTCTAGACTGGTTTTCTTGTTTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_repressed

Protein Analysis

195

Amino Acids

22.06

Weight (kDa)

6.3

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 5 - 88 2.1e-09 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 408
Acc16I TGCGCA 1 cut(s) 71
AciI CCGC 1 cut(s) 444
AclI AACGTT 1 cut(s) 21
AclWI GGATC 1 cut(s) 240
AcuI CTGAAG 1 cut(s) 526
AcyI GRCGYC 1 cut(s) 405
AfaI GTAC 1 cut(s) 351
AfiI CCNNNNNNNGG 1 cut(s) 514
AgsI TTSAA 4 cut(s) 218, 224, 276, 521
AjnI CCWGG 1 cut(s) 461
AloI GAACNNNNNNTCC 2 cut(s) 256, 288
AluBI AGCT 2 cut(s) 65, 116
AluI AGCT 2 cut(s) 65, 116
Alw21I GWGCWC 2 cut(s) 306, 330
Alw26I GTCTC 1 cut(s) 85
AlwI GGATC 1 cut(s) 240
Ama87I CYCGRG 1 cut(s) 508
AoxI GGCC 2 cut(s) 237, 366
ApeKI GCWGC 2 cut(s) 62, 113
Asp700I GAANNNNTTC 1 cut(s) 22
AspLEI GCGC 2 cut(s) 55, 72
AspS9I GGNCC 1 cut(s) 237
AsuC2I CCSGG 2 cut(s) 509, 510
AsuHPI GGTGA 1 cut(s) 363
AvaI CYCGRG 1 cut(s) 508
Bbv12I GWGCWC 2 cut(s) 306, 330
BbvI GCAGC 2 cut(s) 74, 125
BccI CCATC 2 cut(s) 52, 86
BciT130I CCWGG 1 cut(s) 463
BcnI CCSGG 2 cut(s) 509, 510
BcoDI GTCTC 1 cut(s) 85
BfaI CTAG 1 cut(s) 564
BfmI CTRYAG 1 cut(s) 11
BglII AGATCT 1 cut(s) 211
BisI GCNGC 2 cut(s) 63, 114
BlsI GCNGC 2 cut(s) 64, 115
Bme1390I CCNGG 3 cut(s) 463, 509, 510
BmeT110I CYCGRG 1 cut(s) 508
BmgT120I GGNCC 1 cut(s) 237
BmiI GGNNCC 1 cut(s) 460
BmrFI CCNGG 3 cut(s) 463, 509, 510
BmrI ACTGGG 1 cut(s) 341
BmsI GCATC 2 cut(s) 40, 536
BmuI ACTGGG 1 cut(s) 341
Bpu10I CCTNAGC 1 cut(s) 300
BpuEI CTTGAG 1 cut(s) 457
BpuMI CCSGG 2 cut(s) 509, 510
BsaBI GATNNNNATC 1 cut(s) 231
BsaHI GRCGYC 1 cut(s) 405
BsaJI CCNNGG 3 cut(s) 462, 508, 509
BsaXI ACNNNNNCTCC 2 cut(s) 256, 286
Bsc4I CCNNNNNNNGG 1 cut(s) 514
Bse1I ACTGG 2 cut(s) 347, 573
Bse3DI GCAATG 2 cut(s) 79, 98
Bse8I GATNNNNATC 1 cut(s) 231
BseBI CCWGG 1 cut(s) 463
BseDI CCNNGG 3 cut(s) 462, 508, 509
BseGI GGATG 3 cut(s) 97, 319, 532
BseJI GATNNNNATC 1 cut(s) 231
BseLI CCNNNNNNNGG 1 cut(s) 514
BseMI GCAATG 2 cut(s) 79, 98
BseMII CTCAG 1 cut(s) 291
BseNI ACTGG 2 cut(s) 347, 573
BseRI GAGGAG 2 cut(s) 280, 283
BseXI GCAGC 2 cut(s) 74, 125
BseYI CCCAGC 1 cut(s) 133
BshFI GGCC 2 cut(s) 239, 368
BsiHKAI GWGCWC 2 cut(s) 306, 330
BsiHKCI CYCGRG 1 cut(s) 508
BsiSI CCGG 1 cut(s) 509
BslFI GGGAC 1 cut(s) 374
BslI CCNNNNNNNGG 1 cut(s) 514
BsmAI GTCTC 1 cut(s) 85
BsmFI GGGAC 1 cut(s) 374
BsnI GGCC 2 cut(s) 239, 368
BsoBI CYCGRG 1 cut(s) 508
Bsp1286I GDGCHC 2 cut(s) 306, 330
Bsp143I GATC 3 cut(s) 211, 232, 430
BspACI CCGC 1 cut(s) 444
BspANI GGCC 2 cut(s) 239, 368
BspCNI CTCAG 1 cut(s) 292
BspLI GGNNCC 1 cut(s) 460
BspMAI CTGCAG 1 cut(s) 15
BspPI GGATC 1 cut(s) 240
BsrDI GCAATG 2 cut(s) 79, 98
BsrI ACTGG 2 cut(s) 347, 573
BssECI CCNNGG 3 cut(s) 462, 508, 509
BssMI GATC 3 cut(s) 211, 232, 430
BssNI GRCGYC 1 cut(s) 405
Bst2UI CCWGG 1 cut(s) 463
BstACI GRCGYC 1 cut(s) 405
BstC8I GCNNGC 1 cut(s) 551
BstDEI CTNAG 1 cut(s) 300
BstF5I GGATG 3 cut(s) 97, 319, 532
BstHHI GCGC 2 cut(s) 55, 72
BstKTI GATC 3 cut(s) 214, 235, 433
BstMAI GTCTC 1 cut(s) 85
BstMBI GATC 3 cut(s) 211, 232, 430
BstMWI GCNNNNNNNGC 3 cut(s) 59, 164, 441
BstNI CCWGG 1 cut(s) 463
BstSCI CCNGG 3 cut(s) 461, 507, 508
BstSFI CTRYAG 1 cut(s) 11
BstV1I GCAGC 2 cut(s) 74, 125
BstX2I RGATCY 1 cut(s) 211
BstYI RGATCY 1 cut(s) 211
BsuRI GGCC 2 cut(s) 239, 368
BtsCI GGATG 3 cut(s) 97, 319, 532
Cac8I GCNNGC 1 cut(s) 551
CfoI GCGC 2 cut(s) 55, 72
Cfr13I GGNCC 1 cut(s) 237
Cfr9I CCCGGG 1 cut(s) 508
CseI GACGC 1 cut(s) 164
Csp6I GTAC 1 cut(s) 350
CviAII CATG 1 cut(s) 310
CviJI RGCY 6 cut(s) 65, 116, 158, 239, 286, 368
CviKI_1 RGCY 6 cut(s) 65, 116, 158, 239, 286, 368
CviQI GTAC 1 cut(s) 350
DdeI CTNAG 1 cut(s) 300
DpnI GATC 3 cut(s) 213, 234, 432
DpnII GATC 3 cut(s) 211, 232, 430
Eco57I CTGAAG 1 cut(s) 526
Eco88I CYCGRG 1 cut(s) 508
EcoRII CCWGG 1 cut(s) 461
FaeI CATG 1 cut(s) 313
FaiI YATR 7 cut(s) 68, 89, 173, 311, 381, 423, 559
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FaqI GGGAC 1 cut(s) 374
FatI CATG 1 cut(s) 309
Fnu4HI GCNGC 2 cut(s) 63, 114
FokI GGATG 3 cut(s) 104, 326, 539
Fsp4HI GCNGC 2 cut(s) 63, 114
FspBI CTAG 1 cut(s) 564
FspI TGCGCA 1 cut(s) 71
GlaI GCGC 2 cut(s) 54, 71
GluI GCNGC 2 cut(s) 63, 114
GsaI CCCAGC 1 cut(s) 137
HaeIII GGCC 2 cut(s) 239, 368
HapII CCGG 1 cut(s) 509
HgaI GACGC 1 cut(s) 164
HhaI GCGC 2 cut(s) 55, 72
Hin1I GRCGYC 1 cut(s) 405
Hin1II CATG 1 cut(s) 313
Hin6I GCGC 2 cut(s) 53, 70
HinP1I GCGC 2 cut(s) 53, 70
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HinfI GANTC 1 cut(s) 280
HpaII CCGG 1 cut(s) 509
HphI GGTGA 1 cut(s) 363
Hpy166II GTNNAC 1 cut(s) 409
Hpy188I TCNGA 1 cut(s) 545
Hpy188III TCNNGA 3 cut(s) 215, 224, 564
Hpy8I GTNNAC 1 cut(s) 409
HpyAV CCTTC 1 cut(s) 508
HpyCH4IV ACGT 2 cut(s) 21, 405
HpyCH4V TGCA 3 cut(s) 13, 167, 549
HpyF10VI GCNNNNNNNGC 3 cut(s) 59, 164, 441
HpyF3I CTNAG 1 cut(s) 300
HpySE526I ACGT 2 cut(s) 21, 405
Hsp92I GRCGYC 1 cut(s) 405
Hsp92II CATG 1 cut(s) 313
HspAI GCGC 2 cut(s) 53, 70
Kzo9I GATC 3 cut(s) 211, 232, 430
LmnI GCTCC 1 cut(s) 95
LpnPI CCDG 9 cut(s) 53, 147, 312, 360, 402, 448, 475, 522, 554
Lsp1109I GCAGC 2 cut(s) 74, 125
LweI GCATC 2 cut(s) 40, 536
MaeI CTAG 1 cut(s) 564
MaeII ACGT 2 cut(s) 21, 405
MaeIII GTNAC 1 cut(s) 466
MalI GATC 3 cut(s) 213, 234, 432
MboI GATC 3 cut(s) 211, 232, 430
MboII GAAGA 1 cut(s) 134
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 2 cut(s) 306, 330
MluCI AATT 2 cut(s) 168, 387
MnlI CCTC 8 cut(s) 206, 223, 258, 261, 379, 384, 434, 493
MroXI GAANNNNTTC 1 cut(s) 22
MseI TTAA 1 cut(s) 180
MspI CCGG 1 cut(s) 509
MspR9I CCNGG 3 cut(s) 463, 509, 510
MvaI CCWGG 1 cut(s) 463
MwoI GCNNNNNNNGC 3 cut(s) 59, 164, 441
NciI CCSGG 2 cut(s) 509, 510
NdeII GATC 3 cut(s) 211, 232, 430
NlaIII CATG 1 cut(s) 313
NlaIV GGNNCC 1 cut(s) 460
NmuCI GTSAC 1 cut(s) 466
NsbI TGCGCA 1 cut(s) 71
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PdmI GAANNNNTTC 1 cut(s) 22
PfeI GAWTC 1 cut(s) 280
PkrI GCNGC 2 cut(s) 64, 115
Psp1406I AACGTT 1 cut(s) 21
Psp6I CCWGG 1 cut(s) 461
PspFI CCCAGC 1 cut(s) 133
PspGI CCWGG 1 cut(s) 461
PspN4I GGNNCC 1 cut(s) 460
PspPI GGNCC 1 cut(s) 237
PstI CTGCAG 1 cut(s) 15
PsuI RGATCY 1 cut(s) 211
RsaI GTAC 1 cut(s) 351
RsaNI GTAC 1 cut(s) 350
SaqAI TTAA 1 cut(s) 180
SatI GCNGC 2 cut(s) 63, 114
Sau3AI GATC 3 cut(s) 211, 232, 430
Sau96I GGNCC 1 cut(s) 237
ScrFI CCNGG 3 cut(s) 463, 509, 510
SduI GDGCHC 2 cut(s) 306, 330
SetI ASST 8 cut(s) 24, 67, 118, 301, 376, 408, 504, 519
SfaNI GCATC 2 cut(s) 40, 536
SfcI CTRYAG 1 cut(s) 11
SmaI CCCGGG 1 cut(s) 510
SmlI CTYRAG 1 cut(s) 436
SmoI CTYRAG 1 cut(s) 436
Sse9I AATT 2 cut(s) 168, 387
SsiI CCGC 1 cut(s) 444
SspMI CTAG 1 cut(s) 564
StyD4I CCNGG 3 cut(s) 461, 507, 508
TaiI ACGT 2 cut(s) 24, 408
TasI AATT 2 cut(s) 168, 387
TfiI GAWTC 1 cut(s) 280
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TseFI GTSAC 1 cut(s) 466
TseI GCWGC 2 cut(s) 62, 113
Tsp45I GTSAC 1 cut(s) 466
TspDTI ATGAA 3 cut(s) 20, 135, 383
TspMI CCCGGG 1 cut(s) 508
XbaI TCTAGA 1 cut(s) 563
XcmI CCANNNNNNNNNTGG 1 cut(s) 151
XmaI CCCGGG 1 cut(s) 508
XmnI GAANNNNTTC 1 cut(s) 22
XspI CTAG 1 cut(s) 564
ZraI GACGTC 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.