RchiOBHm_Chr2g0146781

Auxin-repressed 12.5 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005839
Physical Location & Seq
Reverse (-)
64428086 .. 64429975
1890 bp
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UTR
Exon/CDS
Intron
PRQ51648

Sequence Viewer

Length: 378 bp
ATGGGTTTTCTTCACAAGCTTTGGGATGAAACACTCGCCGGCCCCGCACCGGAGTCCGGCCTCGGCAAGCTCCGCAAGTACGACTCCCTCTCCGCCCCGCGCACTGCAACTATGGTCGCTGCAGATGAGGCGCCAGTTGTCACTCGGAGCATTACTATTCTTAGGACTCATTCTTCCAACCTTGGAAACTTTCCGGCTGATTCTGGCTCGCCCTCCGAGTCTCCCACCACGCCGGGAACGCCGGTCTCACCGGGAACACCAACAAAAAATTTCAGGAAACTAACAAGGAGAAAATCATCCACGGACGCATTGCGACGCGCCGAGCCTAGAAGTCCGACTGGTTACGATTGGATGGTGATTACTGCATTGGATCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_repressed

Protein Analysis

125

Amino Acids

13.48

Weight (kDa)

9.98

Isoelectric Point (pI)

71.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_repressed PF05564 7 - 119 1.1e-10 Dormancy/auxin associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 130
AccII CGCG 2 cut(s) 100, 318
AciI CCGC 4 cut(s) 45, 73, 93, 98
AcsI RAATTY 1 cut(s) 268
AcyI GRCGYC 1 cut(s) 131
AfaI GTAC 1 cut(s) 80
AfiI CCNNNNNNNGG 2 cut(s) 49, 56
AluBI AGCT 2 cut(s) 19, 70
AluI AGCT 2 cut(s) 19, 70
Alw26I GTCTC 2 cut(s) 225, 250
AoxI GGCC 2 cut(s) 40, 58
ApeKI GCWGC 1 cut(s) 119
ApoI RAATTY 1 cut(s) 268
AspLEI GCGC 3 cut(s) 102, 133, 320
AspS9I GGNCC 1 cut(s) 41
AsuC2I CCSGG 2 cut(s) 234, 252
AsuHPI GGTGA 2 cut(s) 240, 367
BanI GGYRCC 1 cut(s) 130
BbvI GCAGC 1 cut(s) 106
BccI CCATC 1 cut(s) 346
BcnI CCSGG 2 cut(s) 234, 252
BcoDI GTCTC 2 cut(s) 225, 250
BfaI CTAG 1 cut(s) 327
BfmI CTRYAG 1 cut(s) 120
BfoI RGCGCY 1 cut(s) 134
BisI GCNGC 1 cut(s) 120
BlsI GCNGC 1 cut(s) 121
Bme1390I CCNGG 2 cut(s) 234, 252
BmgT120I GGNCC 1 cut(s) 41
BmiI GGNNCC 2 cut(s) 43, 132
BmrFI CCNGG 2 cut(s) 234, 252
BpuMI CCSGG 2 cut(s) 234, 252
BsaHI GRCGYC 1 cut(s) 131
BsaI GGTCTC 1 cut(s) 250
BsaJI CCNNGG 3 cut(s) 61, 181, 300
BsaWI WCCGGW 1 cut(s) 49
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 2 cut(s) 49, 56
Bse118I RCCGGY 2 cut(s) 38, 241
Bse1I ACTGG 2 cut(s) 134, 343
Bse3DI GCAATG 1 cut(s) 308
BseDI CCNNGG 3 cut(s) 61, 181, 300
BseGI GGATG 3 cut(s) 31, 296, 357
BseLI CCNNNNNNNGG 2 cut(s) 49, 56
BseMI GCAATG 1 cut(s) 308
BseNI ACTGG 2 cut(s) 134, 343
BseXI GCAGC 1 cut(s) 106
Bsh1236I CGCG 2 cut(s) 100, 318
BshFI GGCC 2 cut(s) 42, 60
BshNI GGYRCC 1 cut(s) 130
BsiSI CCGG 7 cut(s) 39, 50, 57, 194, 233, 242, 251
BslI CCNNNNNNNGG 2 cut(s) 49, 56
BsmAI GTCTC 2 cut(s) 225, 250
BsnI GGCC 2 cut(s) 42, 60
Bso31I GGTCTC 1 cut(s) 250
Bsp143I GATC 1 cut(s) 370
BspACI CCGC 4 cut(s) 45, 73, 93, 98
BspANI GGCC 2 cut(s) 42, 60
BspFNI CGCG 2 cut(s) 100, 318
BspLI GGNNCC 2 cut(s) 43, 132
BspMAI CTGCAG 1 cut(s) 124
BspT107I GGYRCC 1 cut(s) 130
BspTNI GGTCTC 1 cut(s) 250
BsrDI GCAATG 1 cut(s) 308
BsrFI RCCGGY 2 cut(s) 38, 241
BsrI ACTGG 2 cut(s) 134, 343
BssAI RCCGGY 2 cut(s) 38, 241
BssECI CCNNGG 3 cut(s) 61, 181, 300
BssMI GATC 1 cut(s) 370
BssNI GRCGYC 1 cut(s) 131
BssT1I CCWWGG 1 cut(s) 181
BstACI GRCGYC 1 cut(s) 131
BstC8I GCNNGC 3 cut(s) 40, 68, 209
BstDEI CTNAG 1 cut(s) 161
BstDSI CCRYGG 1 cut(s) 300
BstF5I GGATG 3 cut(s) 31, 296, 357
BstFNI CGCG 2 cut(s) 100, 318
BstH2I RGCGCY 1 cut(s) 134
BstHHI GCGC 3 cut(s) 102, 133, 320
BstKTI GATC 1 cut(s) 373
BstMAI GTCTC 2 cut(s) 225, 250
BstMBI GATC 1 cut(s) 370
BstMWI GCNNNNNNNGC 4 cut(s) 44, 72, 128, 238
BstSCI CCNGG 2 cut(s) 232, 250
BstSFI CTRYAG 1 cut(s) 120
BstUI CGCG 2 cut(s) 100, 318
BstV1I GCAGC 1 cut(s) 106
BsuRI GGCC 2 cut(s) 42, 60
BtgI CCRYGG 1 cut(s) 300
BtsCI GGATG 3 cut(s) 31, 296, 357
BtsI GCAGTG 1 cut(s) 102
BtsIMutI CAGTG 1 cut(s) 102
Cac8I GCNNGC 3 cut(s) 40, 68, 209
CfoI GCGC 3 cut(s) 102, 133, 320
Cfr10I RCCGGY 2 cut(s) 38, 241
Cfr13I GGNCC 1 cut(s) 41
CseI GACGC 2 cut(s) 314, 324
Csp6I GTAC 1 cut(s) 79
CviJI RGCY 7 cut(s) 19, 42, 60, 70, 197, 207, 325
CviKI_1 RGCY 7 cut(s) 19, 42, 60, 70, 197, 207, 325
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 1 cut(s) 161
DinI GGCGCC 1 cut(s) 132
DpnI GATC 1 cut(s) 372
DpnII GATC 1 cut(s) 370
EciI GGCGGA 1 cut(s) 82
Eco130I CCWWGG 1 cut(s) 181
Eco31I GGTCTC 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 181
EgeI GGCGCC 1 cut(s) 132
EheI GGCGCC 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 181
FaiI YATR 1 cut(s) 113
FauI CCCGC 2 cut(s) 52, 105
Fnu4HI GCNGC 1 cut(s) 120
FokI GGATG 3 cut(s) 38, 283, 364
Fsp4HI GCNGC 1 cut(s) 120
FspBI CTAG 1 cut(s) 327
GlaI GCGC 3 cut(s) 101, 132, 319
GluI GCNGC 1 cut(s) 120
HaeII RGCGCY 1 cut(s) 134
HaeIII GGCC 2 cut(s) 42, 60
HapII CCGG 7 cut(s) 39, 50, 57, 194, 233, 242, 251
HgaI GACGC 2 cut(s) 314, 324
HhaI GCGC 3 cut(s) 102, 133, 320
Hin1I GRCGYC 1 cut(s) 131
Hin6I GCGC 3 cut(s) 100, 131, 318
HinP1I GCGC 3 cut(s) 100, 131, 318
HindIII AAGCTT 1 cut(s) 17
HinfI GANTC 5 cut(s) 53, 83, 166, 200, 218
HpaII CCGG 7 cut(s) 39, 50, 57, 194, 233, 242, 251
HphI GGTGA 2 cut(s) 240, 367
Hpy188I TCNGA 3 cut(s) 147, 217, 336
Hpy188III TCNNGA 1 cut(s) 274
Hpy99I CGWCG 1 cut(s) 318
HpyCH4V TGCA 3 cut(s) 107, 122, 365
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 72, 128, 238
HpyF3I CTNAG 1 cut(s) 161
Hsp92I GRCGYC 1 cut(s) 131
HspAI GCGC 3 cut(s) 100, 131, 318
KasI GGCGCC 1 cut(s) 130
KroI GCCGGC 1 cut(s) 38
KroNI GCCGGC 1 cut(s) 40
Kzo9I GATC 1 cut(s) 370
LmnI GCTCC 2 cut(s) 75, 147
Lsp1109I GCAGC 1 cut(s) 106
MaeI CTAG 1 cut(s) 327
MaeIII GTNAC 2 cut(s) 139, 341
MalI GATC 1 cut(s) 372
MboI GATC 1 cut(s) 370
MboII GAAGA 1 cut(s) 165
MluCI AATT 1 cut(s) 268
Mly113I GGCGCC 1 cut(s) 131
MlyI GAGTC 4 cut(s) 62, 77, 160, 227
MmeI TCCRAC 2 cut(s) 201, 359
MnlI CCTC 4 cut(s) 71, 98, 121, 223
MroNI GCCGGC 1 cut(s) 38
MspI CCGG 7 cut(s) 39, 50, 57, 194, 233, 242, 251
MspR9I CCNGG 2 cut(s) 234, 252
MvnI CGCG 2 cut(s) 100, 318
MwoI GCNNNNNNNGC 4 cut(s) 44, 72, 128, 238
NaeI GCCGGC 1 cut(s) 40
NarI GGCGCC 1 cut(s) 131
NciI CCSGG 2 cut(s) 234, 252
NdeII GATC 1 cut(s) 370
NgoMIV GCCGGC 1 cut(s) 38
NlaIV GGNNCC 2 cut(s) 43, 132
NmeAIII GCCGAG 2 cut(s) 42, 346
NmuCI GTSAC 1 cut(s) 139
PdiI GCCGGC 1 cut(s) 40
PfeI GAWTC 1 cut(s) 200
PkrI GCNGC 1 cut(s) 121
PleI GAGTC 4 cut(s) 61, 77, 160, 226
PluTI GGCGCC 1 cut(s) 134
PpsI GAGTC 4 cut(s) 61, 77, 160, 226
PspN4I GGNNCC 2 cut(s) 43, 132
PspPI GGNCC 1 cut(s) 41
PstI CTGCAG 1 cut(s) 124
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
SatI GCNGC 1 cut(s) 120
Sau3AI GATC 1 cut(s) 370
Sau96I GGNCC 1 cut(s) 41
SchI GAGTC 4 cut(s) 62, 77, 160, 227
ScrFI CCNGG 2 cut(s) 234, 252
SetI ASST 3 cut(s) 21, 72, 183
SfcI CTRYAG 1 cut(s) 120
SfoI GGCGCC 1 cut(s) 132
Sse9I AATT 1 cut(s) 268
SsiI CCGC 4 cut(s) 45, 73, 93, 98
SspDI GGCGCC 1 cut(s) 130
SspMI CTAG 1 cut(s) 327
StyD4I CCNGG 2 cut(s) 232, 250
StyI CCWWGG 1 cut(s) 181
TasI AATT 1 cut(s) 268
TfiI GAWTC 1 cut(s) 200
TscAI CASTG 1 cut(s) 109
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 1 cut(s) 119
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 1 cut(s) 42
TspGWI ACGGA 1 cut(s) 317
TspRI CASTG 1 cut(s) 109
XapI RAATTY 1 cut(s) 268
XspI CTAG 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.