pycom17g14350

Auxin-repressed 12.5 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0005839
Physical Location & Seq
Reverse (-)
11855971 .. 11856549
579 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g14350.1

Sequence Viewer

Length: 399 bp
ATGGGTTTCCTTCACAAGCTTTGGGATGAAACACTCGCCGGACCGGCACCCGAATCCGGCCTCGGCAAGCTCCGGAAGTACGACTCTATGTCTATCCCGTCCTCTCCGCCAATGGCTTCGGATGAGGTGCCGGTCACTCGGTGCATTACTATTCTCAGGACTAGTTCGTCCACTCTTGGAAACCTTTCACCTGATTCCGGCTCCCCCTCGGAGTCCCCAACCACACCAGGAACTCCGAGAACCCCTGGAACACCAGGAGCATTGAACTTCAAGAAATTAACAAGGAGGAAATCGTCTGCTGATGCTTTGACACGCGCCGAACCTAGAAGTCCGACTGGTTACGATTGGTTGTTAGCTATGTTTAGAATGATATATGCATATATGTCCTTGCTTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_repressed

Protein Analysis

133

Amino Acids

14.26

Weight (kDa)

9.46

Isoelectric Point (pI)

73.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_repressed PF05564 7 - 118 2.4e-09 Dormancy/auxin associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 166
AccB1I GGYRCC 2 cut(s) 46, 127
AccII CGCG 1 cut(s) 315
AccIII TCCGGA 1 cut(s) 72
AciI CCGC 1 cut(s) 107
AdeI CACNNNGTG 1 cut(s) 141
AfaI GTAC 1 cut(s) 80
AfiI CCNNNNNNNGG 2 cut(s) 56, 197
AgsI TTSAA 2 cut(s) 265, 271
AhdI GACNNNNNGTC 1 cut(s) 88
AhlI ACTAGT 1 cut(s) 161
AjnI CCWGG 3 cut(s) 226, 244, 253
AluBI AGCT 3 cut(s) 19, 70, 356
AluI AGCT 3 cut(s) 19, 70, 356
Aor13HI TCCGGA 1 cut(s) 72
AoxI GGCC 1 cut(s) 58
Asp700I GAANNNNTTC 1 cut(s) 184
AspLEI GCGC 1 cut(s) 317
AspS9I GGNCC 1 cut(s) 41
AsuHPI GGTGA 1 cut(s) 180
AvaII GGWCC 1 cut(s) 41
BanI GGYRCC 2 cut(s) 46, 127
BciT130I CCWGG 3 cut(s) 228, 246, 255
BcuI ACTAGT 1 cut(s) 161
BfaI CTAG 3 cut(s) 162, 324, 397
BglI GCCNNNNNGGC 1 cut(s) 44
Bme1390I CCNGG 3 cut(s) 228, 246, 255
Bme18I GGWCC 1 cut(s) 41
BmeRI GACNNNNNGTC 1 cut(s) 88
BmgT120I GGNCC 1 cut(s) 41
BmiI GGNNCC 3 cut(s) 48, 129, 202
BmrFI CCNGG 3 cut(s) 228, 246, 255
BmsI GCATC 1 cut(s) 292
BsaJI CCNNGG 3 cut(s) 61, 207, 244
BsaWI WCCGGW 1 cut(s) 72
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 197
Bse118I RCCGGY 2 cut(s) 43, 130
Bse1I ACTGG 1 cut(s) 340
BseAI TCCGGA 1 cut(s) 72
BseBI CCWGG 3 cut(s) 228, 246, 255
BseDI CCNNGG 3 cut(s) 61, 207, 244
BseGI GGATG 2 cut(s) 31, 127
BseLI CCNNNNNNNGG 2 cut(s) 56, 197
BseMII CTCAG 1 cut(s) 169
BseNI ACTGG 1 cut(s) 340
Bsh1236I CGCG 1 cut(s) 315
BshFI GGCC 1 cut(s) 60
BshNI GGYRCC 2 cut(s) 46, 127
BsiSI CCGG 6 cut(s) 39, 44, 57, 73, 131, 198
BslFI GGGAC 1 cut(s) 199
BslI CCNNNNNNNGG 2 cut(s) 56, 197
BsmFI GGGAC 1 cut(s) 199
BsnI GGCC 1 cut(s) 60
Bsp13I TCCGGA 1 cut(s) 72
BspACI CCGC 1 cut(s) 107
BspANI GGCC 1 cut(s) 60
BspCNI CTCAG 1 cut(s) 168
BspEI TCCGGA 1 cut(s) 72
BspFNI CGCG 1 cut(s) 315
BspLI GGNNCC 3 cut(s) 48, 129, 202
BspT107I GGYRCC 2 cut(s) 46, 127
BsrFI RCCGGY 2 cut(s) 43, 130
BsrI ACTGG 1 cut(s) 340
BssAI RCCGGY 2 cut(s) 43, 130
BssECI CCNNGG 3 cut(s) 61, 207, 244
Bst2UI CCWGG 3 cut(s) 228, 246, 255
BstC8I GCNNGC 1 cut(s) 68
BstDEI CTNAG 1 cut(s) 155
BstF5I GGATG 2 cut(s) 31, 127
BstFNI CGCG 1 cut(s) 315
BstHHI GCGC 1 cut(s) 317
BstMWI GCNNNNNNNGC 1 cut(s) 44
BstNI CCWGG 3 cut(s) 228, 246, 255
BstSCI CCNGG 3 cut(s) 226, 244, 253
BstUI CGCG 1 cut(s) 315
BsuRI GGCC 1 cut(s) 60
BtsCI GGATG 2 cut(s) 31, 127
Cac8I GCNNGC 1 cut(s) 68
CfoI GCGC 1 cut(s) 317
Cfr10I RCCGGY 2 cut(s) 43, 130
Cfr13I GGNCC 1 cut(s) 41
CpoI CGGWCCG 1 cut(s) 41
Csp6I GTAC 1 cut(s) 79
CspI CGGWCCG 1 cut(s) 41
CviJI RGCY 6 cut(s) 19, 60, 70, 116, 201, 356
CviKI_1 RGCY 6 cut(s) 19, 60, 70, 116, 201, 356
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 1 cut(s) 155
DraIII CACNNNGTG 1 cut(s) 141
DrdI GACNNNNNNGTC 1 cut(s) 166
DriI GACNNNNNGTC 1 cut(s) 88
DseDI GACNNNNNNGTC 1 cut(s) 166
Eam1105I GACNNNNNGTC 1 cut(s) 88
EciI GGCGGA 1 cut(s) 96
Eco47I GGWCC 1 cut(s) 41
EcoRII CCWGG 3 cut(s) 226, 244, 253
EcoT22I ATGCAT 1 cut(s) 379
FaiI YATR 7 cut(s) 89, 359, 373, 375, 379, 381, 383
FaqI GGGAC 1 cut(s) 199
FokI GGATG 2 cut(s) 38, 134
FspBI CTAG 3 cut(s) 162, 324, 397
GlaI GCGC 1 cut(s) 316
HaeIII GGCC 1 cut(s) 60
HapII CCGG 6 cut(s) 39, 44, 57, 73, 131, 198
HhaI GCGC 1 cut(s) 317
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HindIII AAGCTT 1 cut(s) 17
HinfI GANTC 4 cut(s) 53, 83, 194, 212
HpaII CCGG 6 cut(s) 39, 44, 57, 73, 131, 198
HphI GGTGA 1 cut(s) 180
Hpy166II GTNNAC 1 cut(s) 171
Hpy188I TCNGA 4 cut(s) 121, 211, 237, 333
Hpy188III TCNNGA 3 cut(s) 73, 157, 271
Hpy8I GTNNAC 1 cut(s) 171
HpyAV CCTTC 1 cut(s) 20
HpyCH4V TGCA 2 cut(s) 144, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 44
HpyF3I CTNAG 1 cut(s) 155
HspAI GCGC 1 cut(s) 315
Kpn2I TCCGGA 1 cut(s) 72
LmnI GCTCC 3 cut(s) 75, 206, 257
LweI GCATC 1 cut(s) 292
MaeI CTAG 3 cut(s) 162, 324, 397
MaeIII GTNAC 2 cut(s) 133, 338
MluCI AATT 1 cut(s) 275
MlyI GAGTC 2 cut(s) 77, 221
MmeI TCCRAC 1 cut(s) 356
MnlI CCTC 5 cut(s) 71, 112, 118, 217, 279
Mph1103I ATGCAT 1 cut(s) 379
MroI TCCGGA 1 cut(s) 72
MroXI GAANNNNTTC 1 cut(s) 184
MseI TTAA 1 cut(s) 278
MspI CCGG 6 cut(s) 39, 44, 57, 73, 131, 198
MspR9I CCNGG 3 cut(s) 228, 246, 255
MvaI CCWGG 3 cut(s) 228, 246, 255
MvnI CGCG 1 cut(s) 315
MwoI GCNNNNNNNGC 1 cut(s) 44
NlaIV GGNNCC 3 cut(s) 48, 129, 202
NmeAIII GCCGAG 1 cut(s) 42
NmuCI GTSAC 1 cut(s) 133
NsiI ATGCAT 1 cut(s) 379
PdmI GAANNNNTTC 1 cut(s) 184
PfeI GAWTC 2 cut(s) 53, 194
PleI GAGTC 2 cut(s) 77, 220
PpsI GAGTC 2 cut(s) 77, 220
Psp6I CCWGG 3 cut(s) 226, 244, 253
PspGI CCWGG 3 cut(s) 226, 244, 253
PspN4I GGNNCC 3 cut(s) 48, 129, 202
PspPI GGNCC 1 cut(s) 41
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
Rsr2I CGGWCCG 1 cut(s) 41
RsrII CGGWCCG 1 cut(s) 41
SaqAI TTAA 1 cut(s) 278
Sau96I GGNCC 1 cut(s) 41
SchI GAGTC 2 cut(s) 77, 221
ScrFI CCNGG 3 cut(s) 228, 246, 255
SetI ASST 7 cut(s) 21, 72, 129, 186, 193, 325, 358
SfaNI GCATC 1 cut(s) 292
SinI GGWCC 1 cut(s) 41
SpeI ACTAGT 1 cut(s) 161
Sse9I AATT 1 cut(s) 275
SsiI CCGC 1 cut(s) 107
SspMI CTAG 3 cut(s) 162, 324, 397
StyD4I CCNGG 3 cut(s) 226, 244, 253
TasI AATT 1 cut(s) 275
TfiI GAWTC 2 cut(s) 53, 194
Tru1I TTAA 1 cut(s) 278
Tru9I TTAA 1 cut(s) 278
TseFI GTSAC 1 cut(s) 133
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 1 cut(s) 42
VpaK11BI GGWCC 1 cut(s) 41
XmnI GAANNNNTTC 1 cut(s) 184
XspI CTAG 3 cut(s) 162, 324, 397
Zsp2I ATGCAT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.