Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0007573

Physical Location & Seq

Genomic Coordinates Forward (+)

15809032 .. 15813278

Gene Structure 4247 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1161000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2376 bp

ATGTGGTCTGTAAAAGGAGGAAATGGAGCAAAGAAGGGAGCAGGAGGAAATGGATTGGTGGCAGTTGCCATAGACAATCAGAAAGGGAGCCAAAATGCACTCCGATGGGCTGCTGAGCATCTTCTCACCAAGGGCCAAACTGTCATTTTACTACATGTTGTTCAGAAGACATCATCAGTTTGTTCTTCAATAGCTGGAAACAATGCCATTGTATGCAATATAAACAATCTGTCTCAATCACCGCGCAAACAGCAGCTCGAAAAGATGACCAAGGATCTGTTCCTTACCTTCCATTGCTATTGTGCTCGTAAGGATATAAACTGCATTGATATCATACTTGAAGACTCAGATATAGCAAAAGCAGTAACAGAATATGCTTCTTACGCTGCAATTGAGAATTTGGTTCTGGGAGCTCCAACAAAGCATGGATTTATAAGATTCAAATCATCAAGTATTCCAAGCAGTGTATCAAAAGGGGCACCTGATTTCTGCACCGTATATGTCATCTCCAAAGGGAAAATCCACTCTGAGCGACATGCTTCTCGTCCAGCAGCTTACAGTTCTCCACTACTTGCCCAAATAGAGGCATTAAATCAGCAAAGTGCTAAAGCAGCTGAAACACCTAGACAGAATATGTACTTGAAAGCAGCCAGACCATCGTTCAGGCCTCGTAACTTGCCAGATGAAGCTTCCAGATATGCATATACAAGAGGAGGAGGCTTTAGTAATGGAAGGATTAGTGGAGGGTTCTCAGAATCGGAAAGTGAGAGATCATTTATAAGCTCCGACAGGGCAAGCACTGACCGAGCTTCATCTGTGATGTATGAATATATGGACCGAGGGCGGCTATCTACCAGCTCAGACCAAAGCTTTGGATCAATGCGCCTAGGACCTAAGTTCGCAGAACTCAATTTTCCGCAAGACTTCTCGTCAATTTCACATGAAAGCAACCAAACATCATCTTCATGGTCATCAGAGAACCTGGAAGAAGTAGAATCGGAGATGAGGAGGCTAAAGCTAGAGCTGAAGCAAACAATGGACATGTACAGCACGGCATGCAGAGAAGCACTTACAGCTAAACAGAAAGAAATGGAGCTGCACAGCTGGAGGGCTGAAGAGGAACAAAAACTTGAGGAAGCACGATTGGCTCAAGAAGCAGCACAGGCAGTTGCGGAGAGAGAAAAAGCTAGGTGCAAGGCAGCCATAGAAGCAGCTGATGCAGCTAAAAGGATTGCAGAGTTGGAGTCAAATAAACGGGCAAACGCAGAAATTAAGGCTCTTAGAGAAGCAGAGGACATGAGGAAGCTATTATCAAATCTAGCCCACACTGATGACAAGTATAGGAGGTATTCCATTGAGGAGATTGAAGAAGCAACAGAACACTTTTTGCCGTCTCGAAAGATTGGGGAAGGAGGCTATGGTCCTGTCTATAAGTGTTACCTCGATCACACACCGGTTGCAGTCAAGGTCTTGCGTCCGGATGCTGCTCAAGGAAGGTCACAGTTTCAGAAAGAGATCGACATACTTAGCTGCATTCGACATCCCAACATGGTGTTACTTCTAGGAGCCTGTCCAGAGTATGGCATTCTAGTATATGAATACATGGCCAATGGAAGCTTAGATGACTGTCTCTCAAGGAAAGGGAACACCCCAGCTCTGTCTTGGCAACTGAGGTTCCAAATAGCAGCTGAAGTTGCCACAGGCCTACTTTTTCTTCACCAGACCAAGCCGGAACCCTTGGTGCACCGTGACCTCAAGCCGGGCAACATTTTGCTAGATCAAAACTACGTGAGCAAGATTAGTGATGTCGGATTAGCTAGACTTGTCCCAGCTGTTGCTGAAAACGTGACACAGTGCCTCATGACAGCTACGGCCGGAACATTCTGCTATATTGACCCTGAGTATCAGCAGACAGGAATGCTTGGTGTAAAATCTGATGTGTACTCCTTAGGCATTATGCTTCTTCAATTGGTAACAAGTAGGCCACCTATGGGGTTGACTCACCAGGTCGAAACTGCAATTGAAAAGGGCACATTCGCGGGGATGCTAGACCCAGCTGTGCCTGATTGGCCATATGAAGAAGCATTGAGCTTTGCAAAGCTAGCAATCCAATGTGCAGAACTTAGACGAAAGGACAGGCCAGATCTTGGCACTGTGATACTGCCGGCACTGAATAAACTGAGAGAATTTGCTGACGAAAAGATGAACCACAAGTTATTAGGAGGCAATTATAGCCCTTCCCCTAATCACAGTCACGCTGCCGATCAACAAGAAGTAATAAGCGATCCGCAGCTCAAGAACTTGGAAACCTCGAAGAGCCAATCGAGCACATCTTCACAGCCAGAAAATCAAGCAGAAGAAGCAGGTCTATTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr Usp

Protein Analysis

792

Amino Acids

87.32

Weight (kDa)

6.45

Isoelectric Point (pI)

49.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Usp	PF00582	20 - 161	2.1e-08	Universal stress protein family
Pkinase	PF00069	465 - 714	2.8e-40	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	466 - 717	3.4e-38	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007573)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16760 AT1G16760 AT1G78940 AT1G78940 AT1G78940 AT3G20200 AT3G20200 AT3G20200 AT3G20200
fragaria_vesca	FvH4_6g36560
malus_domestica	MD09G1167400.v1.1 MD17G1161000.v1.1
prunus_persica	Prupe.3G025400_v2.0.a1 Prupe.3G025400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0149031
rosa_laevigata	RLG00000020364
rosa_multiflora	Rmu_sc0007360.1_g000003
rosa_roxburghii	Rroxscaffold_2G00098520
rosa_rugosa	Rorug02G0408600 Rorug02G0408700
rosa_samantha	Rh2BG480900 Rh2CG454400 Rh2DG489700
rosa_wichuraiana	Rw2G038130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	434, 779
Acc36I	ACCTGC	1 cut(s)	2354
AccB1I	GGYRCC	1 cut(s)	478
AccB7I	CCANNNNNTGG	2 cut(s)	1580, 2147
AccII	CGCG	2 cut(s)	244, 2039
AccIII	TCCGGA	1 cut(s)	1477
AciI	CCGC	6 cut(s)	242, 844, 917, 1172, 2039, 2288
AclWI	GGATC	3 cut(s)	282, 883, 2279
AcoI	YGGCCR	3 cut(s)	1605, 1872, 2069
AcsI	RAATTY	2 cut(s)	397, 2186
AcuI	CTGAAG	3 cut(s)	1046, 1134, 1710
AfaI	GTAC	3 cut(s)	638, 1046, 1943
AfiI	CCNNNNNNNGG	5 cut(s)	583, 1580, 1759, 1991, 2147
AflIII	ACRYGT	2 cut(s)	154, 1041
AgeI	ACCGGT	1 cut(s)	1453
AgsI	TTSAA	7 cut(s)	189, 341, 442, 643, 1367, 1967, 2024
AhdI	GACNNNNNGTC	1 cut(s)	928
AjnI	CCWGG	2 cut(s)	981, 2004
AjuI	GAANNNNNNNTTGG	2 cut(s)	263, 295
Alw21I	GWGCWC	4 cut(s)	307, 415, 1746, 2330
Alw26I	GTCTC	3 cut(s)	237, 1398, 1634
Alw44I	GTGCAC	1 cut(s)	1742
AlwI	GGATC	3 cut(s)	282, 883, 2279
Aor13HI	TCCGGA	1 cut(s)	1477
AoxI	GGCC	8 cut(s)	133, 665, 1605, 1702, 1872, 1982, 2069, 2138
ApaLI	GTGCAC	1 cut(s)	1742
ApoI	RAATTY	2 cut(s)	397, 2186
ArsI	GACNNNNNNTTYG	2 cut(s)	854, 886
AsiGI	ACCGGT	1 cut(s)	1453
AspA2I	CCTAGG	1 cut(s)	886
AspLEI	GCGC	2 cut(s)	246, 885
AspS9I	GGNCC	4 cut(s)	133, 835, 890, 1421
AsuC2I	CCSGG	1 cut(s)	1761
AsuHPI	GGTGA	4 cut(s)	118, 231, 1709, 1994
AsuNHI	GCTAGC	1 cut(s)	2101
AvaII	GGWCC	3 cut(s)	835, 890, 1421
AvrII	CCTAGG	1 cut(s)	886
AxyI	CCTNAGG	1 cut(s)	1948
BaeGI	GKGCMC	3 cut(s)	481, 1746, 2033
BalI	TGGCCA	2 cut(s)	1607, 2071
BanI	GGYRCC	1 cut(s)	478
BanII	GRGCYC	1 cut(s)	415
BbsI	GAAGAC	2 cut(s)	173, 348
Bbv12I	GWGCWC	4 cut(s)	307, 415, 1746, 2330
BccI	CCATC	2 cut(s)	99, 664
BceAI	ACGGC	3 cut(s)	1068, 1375, 1887
BciT130I	CCWGG	2 cut(s)	983, 2006
BcnI	CCSGG	1 cut(s)	1761
BcoDI	GTCTC	3 cut(s)	237, 1398, 1634
BfuAI	ACCTGC	1 cut(s)	2354
BglI	GCCNNNNNGGC	1 cut(s)	2068
BglII	AGATCT	1 cut(s)	2143
BlnI	CCTAGG	1 cut(s)	886
BlpI	GCTNAGC	1 cut(s)	114
Bme1390I	CCNGG	3 cut(s)	983, 1761, 2006
Bme18I	GGWCC	3 cut(s)	835, 890, 1421
BmeRI	GACNNNNNGTC	1 cut(s)	928
BmgT120I	GGNCC	4 cut(s)	133, 835, 890, 1421
BmiI	GGNNCC	5 cut(s)	89, 480, 1567, 1676, 1734
BmrFI	CCNGG	3 cut(s)	983, 1761, 2006
BmsI	GCATC	4 cut(s)	127, 1207, 1471, 2034
BmtI	GCTAGC	1 cut(s)	2105
BpiI	GAAGAC	2 cut(s)	173, 348
BpmI	CTGGAG	1 cut(s)	1126
Bpu1102I	GCTNAGC	1 cut(s)	114
BpuEI	CTTGAG	6 cut(s)	1134, 1151, 1473, 1618, 1739, 2279
BpuMI	CCSGG	1 cut(s)	1761
BsaAI	YACGTR	1 cut(s)	1789
BsaBI	GATNNNNATC	1 cut(s)	442
BsaJI	CCNNGG	5 cut(s)	129, 270, 838, 886, 1737
BsaWI	WCCGGW	2 cut(s)	1453, 1477
Bsc4I	CCNNNNNNNGG	5 cut(s)	583, 1580, 1759, 1991, 2147
Bse118I	RCCGGY	2 cut(s)	1453, 2164
Bse21I	CCTNAGG	1 cut(s)	1948
Bse3DI	GCAATG	1 cut(s)	292
Bse8I	GATNNNNATC	1 cut(s)	442
BseAI	TCCGGA	1 cut(s)	1477
BseBI	CCWGG	2 cut(s)	983, 2006
BseDI	CCNNGG	5 cut(s)	129, 270, 838, 886, 1737
BseGI	GGATG	3 cut(s)	1486, 1540, 2049
BseJI	GATNNNNATC	1 cut(s)	442
BseLI	CCNNNNNNNGG	5 cut(s)	583, 1580, 1759, 1991, 2147
BseMI	GCAATG	1 cut(s)	292
BseMII	CTCAG	8 cut(s)	105, 360, 519, 765, 873, 1661, 1890, 2171
BseRI	GAGGAG	4 cut(s)	726, 729, 1021, 1373
BseSI	GKGCMC	3 cut(s)	481, 1746, 2033
BseX3I	CGGCCG	1 cut(s)	1872
BseYI	CCCAGC	3 cut(s)	1651, 1828, 2053
BsgI	GTGCAG	3 cut(s)	475, 1082, 2136
Bsh1236I	CGCG	2 cut(s)	244, 2039
Bsh1285I	CGRYCG	1 cut(s)	1875
BshFI	GGCC	8 cut(s)	135, 667, 1607, 1704, 1874, 1984, 2071, 2140
BshNI	GGYRCC	1 cut(s)	478
BshTI	ACCGGT	1 cut(s)	1453
BsiEI	CGRYCG	1 cut(s)	1875
BsiHKAI	GWGCWC	4 cut(s)	307, 415, 1746, 2330
BsiSI	CCGG	6 cut(s)	1454, 1478, 1730, 1760, 1875, 2165
BslFI	GGGAC	1 cut(s)	1811
BslI	CCNNNNNNNGG	5 cut(s)	583, 1580, 1759, 1991, 2147
BsmAI	GTCTC	3 cut(s)	237, 1398, 1634
BsmBI	CGTCTC	1 cut(s)	1398
BsmFI	GGGAC	1 cut(s)	1811
BsmI	GAATGC	3 cut(s)	1533, 1584, 1923
BsnI	GGCC	8 cut(s)	135, 667, 1607, 1704, 1874, 1984, 2071, 2140
Bsp1286I	GDGCHC	6 cut(s)	307, 415, 481, 1746, 2033, 2330
Bsp13I	TCCGGA	1 cut(s)	1477
Bsp1407I	TGTACA	1 cut(s)	1044
Bsp143I	GATC	9 cut(s)	274, 770, 875, 1444, 1515, 1777, 2143, 2263, 2284
Bsp1720I	GCTNAGC	1 cut(s)	114
BspACI	CCGC	6 cut(s)	242, 844, 917, 1172, 2039, 2288
BspANI	GGCC	8 cut(s)	135, 667, 1607, 1704, 1874, 1984, 2071, 2140
BspCNI	CTCAG	8 cut(s)	106, 359, 520, 764, 872, 1662, 1891, 2172
BspEI	TCCGGA	1 cut(s)	1477
BspFNI	CGCG	2 cut(s)	244, 2039
BspHI	TCATGA	1 cut(s)	1860
BspLI	GGNNCC	5 cut(s)	89, 480, 1567, 1676, 1734
BspMI	ACCTGC	1 cut(s)	2354
BspOI	GCTAGC	1 cut(s)	2105
BspPI	GGATC	3 cut(s)	282, 883, 2279
BspQI	GCTCTTC	1 cut(s)	2309
BspT107I	GGYRCC	1 cut(s)	478
BsrDI	GCAATG	1 cut(s)	292
BsrFI	RCCGGY	2 cut(s)	1453, 2164
BsrGI	TGTACA	1 cut(s)	1044
BssAI	RCCGGY	2 cut(s)	1453, 2164
BssECI	CCNNGG	5 cut(s)	129, 270, 838, 886, 1737
BssMI	GATC	9 cut(s)	274, 770, 875, 1444, 1515, 1777, 2143, 2263, 2284
BssT1I	CCWWGG	4 cut(s)	129, 270, 886, 1737
Bst2UI	CCWGG	2 cut(s)	983, 2006
Bst4CI	ACNGT	9 cut(s)	142, 496, 560, 1503, 1628, 1748, 1854, 2155, 2252
Bst6I	CTCTTC	2 cut(s)	1110, 2309
BstAPI	GCANNNNNTGC	2 cut(s)	1056, 1217
BstAUI	TGTACA	1 cut(s)	1044
BstBAI	YACGTR	1 cut(s)	1789
BstC8I	GCNNGC	4 cut(s)	796, 1057, 2103, 2166
BstF5I	GGATG	3 cut(s)	1486, 1540, 2049
BstFNI	CGCG	2 cut(s)	244, 2039
BstHHI	GCGC	2 cut(s)	246, 885
BstKTI	GATC	9 cut(s)	277, 773, 878, 1447, 1518, 1780, 2146, 2266, 2287
BstMAI	GTCTC	3 cut(s)	237, 1398, 1634
BstMBI	GATC	9 cut(s)	274, 770, 875, 1444, 1515, 1777, 2143, 2263, 2284
BstMCI	CGRYCG	1 cut(s)	1875
BstNI	CCWGG	2 cut(s)	983, 2006
BstNSI	RCATGY	4 cut(s)	158, 539, 1045, 1059
BstSCI	CCNGG	3 cut(s)	981, 1759, 2004
BstSLI	GKGCMC	3 cut(s)	481, 1746, 2033
BstUI	CGCG	2 cut(s)	244, 2039
BstV2I	GAAGAC	2 cut(s)	173, 348
BstX2I	RGATCY	2 cut(s)	274, 2143
BstXI	CCANNNNNNTGG	1 cut(s)	872
BstYI	RGATCY	2 cut(s)	274, 2143
BstZI	CGGCCG	1 cut(s)	1872
Bsu36I	CCTNAGG	1 cut(s)	1948
BsuRI	GGCC	8 cut(s)	135, 667, 1607, 1704, 1874, 1984, 2071, 2140
BtsCI	GGATG	3 cut(s)	1486, 1540, 2049
BtsI	GCAGTG	1 cut(s)	469
BtsIMutI	CAGTG	6 cut(s)	469, 798, 1326, 1859, 2151, 2168
BveI	ACCTGC	1 cut(s)	2354
Cac8I	GCNNGC	4 cut(s)	796, 1057, 2103, 2166
CciI	TCATGA	1 cut(s)	1860
CfoI	GCGC	2 cut(s)	246, 885
Cfr10I	RCCGGY	2 cut(s)	1453, 2164
Cfr13I	GGNCC	4 cut(s)	133, 835, 890, 1421
CseI	GACGC	1 cut(s)	1463
CsiI	ACCWGGT	1 cut(s)	2004
Csp6I	GTAC	3 cut(s)	637, 1045, 1942
CspAI	ACCGGT	1 cut(s)	1453
CviQI	GTAC	3 cut(s)	637, 1045, 1942
DpnI	GATC	9 cut(s)	276, 772, 877, 1446, 1517, 1779, 2145, 2265, 2286
DpnII	GATC	9 cut(s)	274, 770, 875, 1444, 1515, 1777, 2143, 2263, 2284
DriI	GACNNNNNGTC	1 cut(s)	928
EaeI	YGGCCR	3 cut(s)	1605, 1872, 2069
EagI	CGGCCG	1 cut(s)	1872
Eam1104I	CTCTTC	2 cut(s)	1110, 2309
Eam1105I	GACNNNNNGTC	1 cut(s)	928
EarI	CTCTTC	2 cut(s)	1110, 2309
Ecl136II	GAGCTC	1 cut(s)	413
EclXI	CGGCCG	1 cut(s)	1872
Eco130I	CCWWGG	4 cut(s)	129, 270, 886, 1737
Eco147I	AGGCCT	2 cut(s)	667, 1704
Eco24I	GRGCYC	1 cut(s)	415
Eco32I	GATATC	1 cut(s)	331
Eco47I	GGWCC	3 cut(s)	835, 890, 1421
Eco52I	CGGCCG	1 cut(s)	1872
Eco53kI	GAGCTC	1 cut(s)	413
Eco57I	CTGAAG	3 cut(s)	1046, 1134, 1710
Eco81I	CCTNAGG	1 cut(s)	1948
EcoICRI	GAGCTC	1 cut(s)	413
EcoO109I	RGGNCCY	1 cut(s)	890
EcoRII	CCWGG	2 cut(s)	981, 2004
EcoRV	GATATC	1 cut(s)	331
EcoT14I	CCWWGG	4 cut(s)	129, 270, 886, 1737
EcoT22I	ATGCAT	1 cut(s)	703
EcoT38I	GRGCYC	1 cut(s)	415
ErhI	CCWWGG	4 cut(s)	129, 270, 886, 1737
Esp3I	CGTCTC	1 cut(s)	1398
FaqI	GGGAC	1 cut(s)	1811
FauI	CCCGC	1 cut(s)	2032
FauNDI	CATATG	1 cut(s)	2074
FokI	GGATG	3 cut(s)	1493, 1527, 2056
FriOI	GRGCYC	1 cut(s)	415
GlaI	GCGC	2 cut(s)	245, 884
GsaI	CCCAGC	3 cut(s)	1655, 1832, 2057
GsuI	CTGGAG	1 cut(s)	1126
HaeIII	GGCC	8 cut(s)	135, 667, 1607, 1704, 1874, 1984, 2071, 2140
HapII	CCGG	6 cut(s)	1454, 1478, 1730, 1760, 1875, 2165
HgaI	GACGC	1 cut(s)	1463
HhaI	GCGC	2 cut(s)	246, 885
Hin6I	GCGC	2 cut(s)	244, 883
HinP1I	GCGC	2 cut(s)	244, 883
HincII	GTYRAC	1 cut(s)	1998
HindII	GTYRAC	1 cut(s)	1998
HindIII	AAGCTT	3 cut(s)	687, 868, 1615
HinfI	GANTC	6 cut(s)	344, 438, 755, 995, 1244, 1999
HpaII	CCGG	6 cut(s)	1454, 1478, 1730, 1760, 1875, 2165
HphI	GGTGA	4 cut(s)	118, 231, 1709, 1994
Hpy166II	GTNNAC	3 cut(s)	1744, 1942, 1998
Hpy188III	TCNNGA	7 cut(s)	693, 1151, 1395, 1478, 1574, 1861, 2296
Hpy8I	GTNNAC	3 cut(s)	1744, 1942, 1998
HpyAV	CCTTC	6 cut(s)	28, 298, 726, 1403, 1488, 2247
HpyCH4III	ACNGT	9 cut(s)	142, 496, 560, 1503, 1628, 1748, 1854, 2155, 2252
HpyCH4IV	ACGT	2 cut(s)	1788, 1845
HpySE526I	ACGT	2 cut(s)	1788, 1845
HspAI	GCGC	2 cut(s)	244, 883
Kpn2I	TCCGGA	1 cut(s)	1477
KroI	GCCGGC	1 cut(s)	2164
KroNI	GCCGGC	1 cut(s)	2166
Kzo9I	GATC	9 cut(s)	274, 770, 875, 1444, 1515, 1777, 2143, 2263, 2284
LguI	GCTCTTC	1 cut(s)	2309
LmnI	GCTCC	8 cut(s)	26, 38, 87, 410, 418, 788, 1093, 1565
LweI	GCATC	4 cut(s)	127, 1207, 1471, 2034
MabI	ACCWGGT	1 cut(s)	2004
MaeII	ACGT	2 cut(s)	1788, 1845
MaeIII	GTNAC	9 cut(s)	364, 671, 1436, 1497, 1554, 1748, 1846, 1972, 2252
MalI	GATC	9 cut(s)	276, 772, 877, 1446, 1517, 1779, 2145, 2265, 2286
MboI	GATC	9 cut(s)	274, 770, 875, 1444, 1515, 1777, 2143, 2263, 2284
MfeI	CAATTG	3 cut(s)	390, 1967, 2019
MflI	RGATCY	2 cut(s)	274, 2143
MhlI	GDGCHC	6 cut(s)	307, 415, 481, 1746, 2033, 2330
MlsI	TGGCCA	2 cut(s)	1607, 2071
MluCI	AATT	9 cut(s)	390, 397, 910, 933, 1269, 1967, 2019, 2186, 2227
MluNI	TGGCCA	2 cut(s)	1607, 2071
MlyI	GAGTC	3 cut(s)	338, 1253, 1993
MmeI	TCCRAC	4 cut(s)	440, 810, 1221, 1789
Mox20I	TGGCCA	2 cut(s)	1607, 2071
Mph1103I	ATGCAT	1 cut(s)	703
MroI	TCCGGA	1 cut(s)	1477
MroNI	GCCGGC	1 cut(s)	2164
MscI	TGGCCA	2 cut(s)	1607, 2071
MseI	TTAA	2 cut(s)	590, 1272
MslI	CAYNNNNRTG	3 cut(s)	103, 964, 1329
Msp20I	TGGCCA	2 cut(s)	1607, 2071
MspA1I	CMGCKG	6 cut(s)	614, 1104, 1214, 1688, 1832, 2057
MspI	CCGG	6 cut(s)	1454, 1478, 1730, 1760, 1875, 2165
MspR9I	CCNGG	3 cut(s)	983, 1761, 2006
MunI	CAATTG	3 cut(s)	390, 1967, 2019
Mva1269I	GAATGC	3 cut(s)	1533, 1584, 1923
MvaI	CCWGG	2 cut(s)	983, 2006
MvnI	CGCG	2 cut(s)	244, 2039
NaeI	GCCGGC	1 cut(s)	2166
NciI	CCSGG	1 cut(s)	1761
NdeI	CATATG	1 cut(s)	2074
NdeII	GATC	9 cut(s)	274, 770, 875, 1444, 1515, 1777, 2143, 2263, 2284
NgoMIV	GCCGGC	1 cut(s)	2164
NheI	GCTAGC	1 cut(s)	2101
NlaIV	GGNNCC	5 cut(s)	89, 480, 1567, 1676, 1734
NmuCI	GTSAC	4 cut(s)	1497, 1748, 1846, 2252
NsiI	ATGCAT	1 cut(s)	703
NspI	RCATGY	4 cut(s)	158, 539, 1045, 1059
PaeI	GCATGC	1 cut(s)	1059
PagI	TCATGA	1 cut(s)	1860
PceI	AGGCCT	2 cut(s)	667, 1704
PciI	ACATGT	2 cut(s)	154, 1041
PciSI	GCTCTTC	1 cut(s)	2309
PctI	GAATGC	3 cut(s)	1533, 1584, 1923
PdiI	GCCGGC	1 cut(s)	2166
PfeI	GAWTC	3 cut(s)	438, 755, 995
PflMI	CCANNNNNTGG	2 cut(s)	1580, 2147
PinAI	ACCGGT	1 cut(s)	1453
PleI	GAGTC	3 cut(s)	338, 1252, 1993
PpsI	GAGTC	3 cut(s)	338, 1252, 1993
Ppu21I	YACGTR	1 cut(s)	1789
PpuMI	RGGWCCY	1 cut(s)	890
PscI	ACATGT	2 cut(s)	154, 1041
PsiI	TTATAA	2 cut(s)	434, 779
Psp124BI	GAGCTC	1 cut(s)	415
Psp5II	RGGWCCY	1 cut(s)	890
Psp6I	CCWGG	2 cut(s)	981, 2004
PspFI	CCCAGC	3 cut(s)	1651, 1828, 2053
PspGI	CCWGG	2 cut(s)	981, 2004
PspN4I	GGNNCC	5 cut(s)	89, 480, 1567, 1676, 1734
PspPI	GGNCC	4 cut(s)	133, 835, 890, 1421
PspPPI	RGGWCCY	1 cut(s)	890
PsuI	RGATCY	2 cut(s)	274, 2143
PvuII	CAGCTG	6 cut(s)	614, 1104, 1214, 1688, 1832, 2057
RsaI	GTAC	3 cut(s)	638, 1046, 1943
RsaNI	GTAC	3 cut(s)	637, 1045, 1942
RseI	CAYNNNNRTG	3 cut(s)	103, 964, 1329
SacI	GAGCTC	1 cut(s)	415
SapI	GCTCTTC	1 cut(s)	2309
SaqAI	TTAA	2 cut(s)	590, 1272
Sau3AI	GATC	9 cut(s)	274, 770, 875, 1444, 1515, 1777, 2143, 2263, 2284
Sau96I	GGNCC	4 cut(s)	133, 835, 890, 1421
SchI	GAGTC	3 cut(s)	338, 1253, 1993
ScrFI	CCNGG	3 cut(s)	983, 1761, 2006
SduI	GDGCHC	6 cut(s)	307, 415, 481, 1746, 2033, 2330
SexAI	ACCWGGT	1 cut(s)	2004
SfaNI	GCATC	4 cut(s)	127, 1207, 1471, 2034
SinI	GGWCC	3 cut(s)	835, 890, 1421
SmiMI	CAYNNNNRTG	3 cut(s)	103, 964, 1329
SmlI	CTYRAG	6 cut(s)	1130, 1149, 1488, 1633, 1754, 2294
SmoI	CTYRAG	6 cut(s)	1130, 1149, 1488, 1633, 1754, 2294
SphI	GCATGC	1 cut(s)	1059
Sse9I	AATT	9 cut(s)	390, 397, 910, 933, 1269, 1967, 2019, 2186, 2227
SseBI	AGGCCT	2 cut(s)	667, 1704
SsiI	CCGC	6 cut(s)	242, 844, 917, 1172, 2039, 2288
SstI	GAGCTC	1 cut(s)	415
StuI	AGGCCT	2 cut(s)	667, 1704
StyD4I	CCNGG	3 cut(s)	981, 1759, 2004
StyI	CCWWGG	4 cut(s)	129, 270, 886, 1737
TaaI	ACNGT	9 cut(s)	142, 496, 560, 1503, 1628, 1748, 1854, 2155, 2252
TaiI	ACGT	2 cut(s)	1791, 1848
TaqI	TCGA	8 cut(s)	258, 1396, 1443, 1518, 1537, 2010, 2312, 2324
TaqII	GACCGA	2 cut(s)	819, 852
TasI	AATT	9 cut(s)	390, 397, 910, 933, 1269, 1967, 2019, 2186, 2227
TatI	WGTACW	3 cut(s)	636, 1044, 1941
TauI	GCSGC	1 cut(s)	847
TfiI	GAWTC	3 cut(s)	438, 755, 995
Tru1I	TTAA	2 cut(s)	590, 1272
Tru9I	TTAA	2 cut(s)	590, 1272
TscAI	CASTG	6 cut(s)	469, 805, 1333, 1859, 2158, 2175
TseFI	GTSAC	4 cut(s)	1497, 1748, 1846, 2252
Tsp45I	GTSAC	4 cut(s)	1497, 1748, 1846, 2252
TspDTI	ATGAA	8 cut(s)	699, 801, 840, 954, 957, 1611, 2091, 2219
TspRI	CASTG	6 cut(s)	469, 805, 1333, 1859, 2158, 2175
Van91I	CCANNNNNTGG	2 cut(s)	1580, 2147
VneI	GTGCAC	1 cut(s)	1742
VpaK11BI	GGWCC	3 cut(s)	835, 890, 1421
XapI	RAATTY	2 cut(s)	397, 2186
XceI	RCATGY	4 cut(s)	158, 539, 1045, 1059
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1659
XmaJI	CCTAGG	1 cut(s)	886
Zsp2I	ATGCAT	1 cut(s)	703

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.