MD17G1162500.v1.1

plastid-lipid-associated protein 10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008453
Physical Location & Seq
Reverse (-)
16073584 .. 16076908
3325 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1162500.v1.1.491

Sequence Viewer

Length: 840 bp
ATGGAACTAGCACTTTGTTCTCCATTGTTCCCTTCAAATGCAACAAGAGGTGTCAATAAGATTAAGCCTTATAGCTCCATGCTCATTGCCTCACAAAAGTTGTCCGCCCAGAAATCGTTTCCATGTCTCGCAGCTGTTGCTACCCAAGCAGCCCAGACGGTGGAGCTTGAGGTAGAGAGCAAGAAACATGAGCTGTTGAGAGCTATACAAGACACCGAAAGGGGCCTTGTTACAACCGATGATCAACGCTCATTTATAGAGGAGGCTCTGGTGAGTGTGGAGGGCTATAACATGGGTGCACCAATAGACTTGGTGAAATTGGATGGGACGTGGCGTTTGCAATATACTTCCGCTTCTGACGTTCTCATTCTTCTGGAAGCGGCTGAAAGGCTACCATTTTTTCAGGTTGGGCAAATTTTTCAGAAATTTGAATGCAAAGACCAGACCAACGGGGGAGTGATCCGTAATGTTGTCAGATGGAGCATTCCACCTTTGTTAGAGGAACAAGAAGGTGCGACTCTGCTAGTTTCTGCAAAATTTTCGGTTGTTTCTGTGCGTAACATCTACCTTCAGTTCAAAGAGATTAATGTTCAAGATATTAAAATCAGTGAAGAGCTGCAGGCTCTAATAGCTCCAGCAATATTGCCACGATCGTTTTTAAGTTTACAGATCTTGCAGTACCTCCGGACTTTCAAAGCGCAAATTCCCGTTAGAGATCCAGGAAGGCAATCGGTCGGAGGACTATATTACCTTTCTTACTTGGACGCTAATATGCTTTTGGGGCGCGCGGTTGGTGGCGGAGGAGTTTTTGTTTTCACGAAAGCTCAACCTCTGAAGTAA

Protein Analysis

280

Amino Acids

30.9

Weight (kDa)

7.64

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 61 - 272 1.1e-23 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 160
AccII CGCG 2 cut(s) 786, 788
AccIII TCCGGA 1 cut(s) 684
AciI CCGC 5 cut(s) 105, 351, 380, 788, 798
AclWI GGATC 2 cut(s) 454, 710
AcsI RAATTY 4 cut(s) 414, 425, 536, 702
AcuI CTGAAG 1 cut(s) 554
AfaI GTAC 1 cut(s) 680
AfiI CCNNNNNNNGG 1 cut(s) 160
AgsI TTSAA 5 cut(s) 36, 431, 577, 593, 694
AjiI CACGTC 1 cut(s) 330
AjnI CCWGG 1 cut(s) 718
AluBI AGCT 8 cut(s) 75, 134, 166, 193, 203, 616, 632, 824
AluI AGCT 8 cut(s) 75, 134, 166, 193, 203, 616, 632, 824
Alw21I GWGCWC 1 cut(s) 301
Alw26I GTCTC 1 cut(s) 131
Alw44I GTGCAC 1 cut(s) 297
AlwI GGATC 2 cut(s) 454, 710
Aor13HI TCCGGA 1 cut(s) 684
AoxI GGCC 1 cut(s) 223
ApaLI GTGCAC 1 cut(s) 297
ApeKI GCWGC 3 cut(s) 131, 149, 616
ApoI RAATTY 4 cut(s) 414, 425, 536, 702
ArsI GACNNNNNNTTYG 2 cut(s) 319, 351
AseI ATTAAT 1 cut(s) 585
AspLEI GCGC 3 cut(s) 700, 786, 788
AspS9I GGNCC 1 cut(s) 223
AsuHPI GGTGA 2 cut(s) 283, 325
BaeGI GKGCMC 1 cut(s) 301
Bbv12I GWGCWC 1 cut(s) 301
BbvI GCAGC 3 cut(s) 143, 161, 603
BccI CCATC 2 cut(s) 317, 471
BcgI CGANNNNNNTGC 2 cut(s) 522, 556
BciT130I CCWGG 1 cut(s) 720
BclI TGATCA 1 cut(s) 241
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 2 cut(s) 8, 524
BfmI CTRYAG 1 cut(s) 617
BglII AGATCT 1 cut(s) 669
BisI GCNGC 4 cut(s) 132, 150, 381, 617
BlsI GCNGC 4 cut(s) 133, 151, 382, 618
Bme1390I CCNGG 1 cut(s) 720
BmgBI CACGTC 1 cut(s) 330
BmgT120I GGNCC 1 cut(s) 223
BmiI GGNNCC 1 cut(s) 224
BmrFI CCNGG 1 cut(s) 720
BpmI CTGGAG 1 cut(s) 618
BpuEI CTTGAG 1 cut(s) 188
BsaWI WCCGGW 1 cut(s) 684
BsaXI ACNNNNNCTCC 2 cut(s) 795, 825
Bsc4I CCNNNNNNNGG 1 cut(s) 160
Bse3DI GCAATG 1 cut(s) 84
BseAI TCCGGA 1 cut(s) 684
BseBI CCWGG 1 cut(s) 720
BseGI GGATG 1 cut(s) 328
BseLI CCNNNNNNNGG 1 cut(s) 160
BseMI GCAATG 1 cut(s) 84
BsePI GCGCGC 1 cut(s) 784
BseRI GAGGAG 2 cut(s) 275, 816
BseSI GKGCMC 1 cut(s) 301
BseXI GCAGC 3 cut(s) 143, 161, 603
Bsh1236I CGCG 2 cut(s) 786, 788
Bsh1285I CGRYCG 2 cut(s) 653, 735
BshFI GGCC 1 cut(s) 225
BsiEI CGRYCG 2 cut(s) 653, 735
BsiHKAI GWGCWC 1 cut(s) 301
BsiSI CCGG 1 cut(s) 685
BslFI GGGAC 1 cut(s) 340
BslI CCNNNNNNNGG 1 cut(s) 160
BsmAI GTCTC 1 cut(s) 131
BsmFI GGGAC 1 cut(s) 340
BsmI GAATGC 2 cut(s) 437, 483
BsnI GGCC 1 cut(s) 225
Bsp1286I GDGCHC 1 cut(s) 301
Bsp13I TCCGGA 1 cut(s) 684
Bsp143I GATC 5 cut(s) 241, 459, 650, 669, 715
BspACI CCGC 5 cut(s) 105, 351, 380, 788, 798
BspANI GGCC 1 cut(s) 225
BspEI TCCGGA 1 cut(s) 684
BspFNI CGCG 2 cut(s) 786, 788
BspLI GGNNCC 1 cut(s) 224
BspMAI CTGCAG 1 cut(s) 621
BspPI GGATC 2 cut(s) 454, 710
BspQI GCTCTTC 1 cut(s) 606
BsrDI GCAATG 1 cut(s) 84
BssHII GCGCGC 1 cut(s) 784
BssMI GATC 5 cut(s) 241, 459, 650, 669, 715
Bst2UI CCWGG 1 cut(s) 720
Bst4CI ACNGT 1 cut(s) 160
Bst6I CTCTTC 1 cut(s) 606
BstAPI GCANNNNNTGC 1 cut(s) 137
BstC8I GCNNGC 2 cut(s) 621, 786
BstF5I GGATG 1 cut(s) 328
BstFNI CGCG 2 cut(s) 786, 788
BstHHI GCGC 3 cut(s) 700, 786, 788
BstKTI GATC 5 cut(s) 244, 462, 653, 672, 718
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 5 cut(s) 241, 459, 650, 669, 715
BstMCI CGRYCG 2 cut(s) 653, 735
BstMWI GCNNNNNNNGC 4 cut(s) 137, 146, 629, 781
BstNI CCWGG 1 cut(s) 720
BstSCI CCNGG 1 cut(s) 718
BstSFI CTRYAG 1 cut(s) 617
BstSLI GKGCMC 1 cut(s) 301
BstUI CGCG 2 cut(s) 786, 788
BstV1I GCAGC 3 cut(s) 143, 161, 603
BstX2I RGATCY 2 cut(s) 669, 715
BstYI RGATCY 2 cut(s) 669, 715
BsuRI GGCC 1 cut(s) 225
BtrI CACGTC 1 cut(s) 330
BtsCI GGATG 1 cut(s) 328
BtsIMutI CAGTG 1 cut(s) 613
Cac8I GCNNGC 2 cut(s) 621, 786
CfoI GCGC 3 cut(s) 700, 786, 788
Cfr13I GGNCC 1 cut(s) 223
CseI GACGC 1 cut(s) 773
Csp6I GTAC 1 cut(s) 679
CviAII CATG 4 cut(s) 79, 123, 188, 292
CviQI GTAC 1 cut(s) 679
DpnI GATC 5 cut(s) 243, 461, 652, 671, 717
DpnII GATC 5 cut(s) 241, 459, 650, 669, 715
Eam1104I CTCTTC 1 cut(s) 606
EarI CTCTTC 1 cut(s) 606
EciI GGCGGA 2 cut(s) 94, 813
Eco57I CTGAAG 1 cut(s) 554
EcoO109I RGGNCCY 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 718
FaeI CATG 4 cut(s) 82, 126, 191, 295
FaqI GGGAC 1 cut(s) 340
FatI CATG 4 cut(s) 78, 122, 187, 291
FbaI TGATCA 1 cut(s) 241
Fnu4HI GCNGC 4 cut(s) 132, 150, 381, 617
FokI GGATG 1 cut(s) 335
Fsp4HI GCNGC 4 cut(s) 132, 150, 381, 617
FspBI CTAG 2 cut(s) 8, 524
GlaI GCGC 3 cut(s) 699, 785, 787
GluI GCNGC 4 cut(s) 132, 150, 381, 617
GsuI CTGGAG 1 cut(s) 618
HaeIII GGCC 1 cut(s) 225
HapII CCGG 1 cut(s) 685
HgaI GACGC 1 cut(s) 773
HhaI GCGC 3 cut(s) 700, 786, 788
Hin1II CATG 4 cut(s) 82, 126, 191, 295
Hin6I GCGC 3 cut(s) 698, 784, 786
HinP1I GCGC 3 cut(s) 698, 784, 786
HinfI GANTC 1 cut(s) 517
HpaII CCGG 1 cut(s) 685
HphI GGTGA 2 cut(s) 283, 325
Hpy166II GTNNAC 2 cut(s) 299, 665
Hpy188I TCNGA 5 cut(s) 358, 423, 476, 737, 834
Hpy188III TCNNGA 4 cut(s) 374, 593, 685, 817
Hpy8I GTNNAC 2 cut(s) 299, 665
HpyAV CCTTC 4 cut(s) 42, 503, 578, 717
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4IV ACGT 2 cut(s) 329, 360
HpyCH4V TGCA 7 cut(s) 41, 299, 340, 435, 533, 619, 676
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 146, 629, 781
HpySE526I ACGT 2 cut(s) 329, 360
Hsp92II CATG 4 cut(s) 82, 126, 191, 295
HspAI GCGC 3 cut(s) 698, 784, 786
Kpn2I TCCGGA 1 cut(s) 684
Ksp22I TGATCA 1 cut(s) 241
Kzo9I GATC 5 cut(s) 241, 459, 650, 669, 715
LguI GCTCTTC 1 cut(s) 606
LmnI GCTCC 4 cut(s) 80, 163, 480, 637
Lsp1109I GCAGC 3 cut(s) 143, 161, 603
MaeI CTAG 2 cut(s) 8, 524
MaeII ACGT 2 cut(s) 329, 360
MaeIII GTNAC 2 cut(s) 229, 557
MalI GATC 5 cut(s) 243, 461, 652, 671, 717
MboI GATC 5 cut(s) 241, 459, 650, 669, 715
MboII GAAGA 2 cut(s) 362, 623
MflI RGATCY 2 cut(s) 669, 715
MhlI GDGCHC 1 cut(s) 301
MluCI AATT 5 cut(s) 317, 414, 425, 536, 702
MlyI GAGTC 1 cut(s) 511
MmeI TCCRAC 1 cut(s) 715
MroI TCCGGA 1 cut(s) 684
MseI TTAA 4 cut(s) 63, 585, 600, 659
MspA1I CMGCKG 1 cut(s) 134
MspI CCGG 1 cut(s) 685
MspR9I CCNGG 1 cut(s) 720
Mva1269I GAATGC 2 cut(s) 437, 483
MvaI CCWGG 1 cut(s) 720
MvnI CGCG 2 cut(s) 786, 788
MwoI GCNNNNNNNGC 4 cut(s) 137, 146, 629, 781
NdeII GATC 5 cut(s) 241, 459, 650, 669, 715
NlaIII CATG 4 cut(s) 82, 126, 191, 295
NlaIV GGNNCC 1 cut(s) 224
PauI GCGCGC 1 cut(s) 784
PciSI GCTCTTC 1 cut(s) 606
PctI GAATGC 2 cut(s) 437, 483
PflMI CCANNNNNTGG 1 cut(s) 160
PfoI TCCNGGA 1 cut(s) 718
PkrI GCNGC 4 cut(s) 133, 151, 382, 618
Ple19I CGATCG 1 cut(s) 653
PleI GAGTC 1 cut(s) 511
PpsI GAGTC 1 cut(s) 511
PshBI ATTAAT 1 cut(s) 585
Psp6I CCWGG 1 cut(s) 718
PspGI CCWGG 1 cut(s) 718
PspN4I GGNNCC 1 cut(s) 224
PspPI GGNCC 1 cut(s) 223
PstI CTGCAG 1 cut(s) 621
PsuI RGATCY 2 cut(s) 669, 715
PteI GCGCGC 1 cut(s) 784
PvuI CGATCG 1 cut(s) 653
PvuII CAGCTG 1 cut(s) 134
RsaI GTAC 1 cut(s) 680
RsaNI GTAC 1 cut(s) 679
SapI GCTCTTC 1 cut(s) 606
SaqAI TTAA 4 cut(s) 63, 585, 600, 659
SatI GCNGC 4 cut(s) 132, 150, 381, 617
Sau3AI GATC 5 cut(s) 241, 459, 650, 669, 715
Sau96I GGNCC 1 cut(s) 223
SchI GAGTC 1 cut(s) 511
ScrFI CCNGG 1 cut(s) 720
SduI GDGCHC 1 cut(s) 301
SfcI CTRYAG 1 cut(s) 617
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
Sse9I AATT 5 cut(s) 317, 414, 425, 536, 702
SsiI CCGC 5 cut(s) 105, 351, 380, 788, 798
SspI AATATT 1 cut(s) 642
SspMI CTAG 2 cut(s) 8, 524
StyD4I CCNGG 1 cut(s) 718
TaaI ACNGT 1 cut(s) 160
TaiI ACGT 2 cut(s) 332, 363
TaqII GACCGA 1 cut(s) 721
TasI AATT 5 cut(s) 317, 414, 425, 536, 702
TauI GCSGC 1 cut(s) 383
Tru1I TTAA 4 cut(s) 63, 585, 600, 659
Tru9I TTAA 4 cut(s) 63, 585, 600, 659
TscAI CASTG 1 cut(s) 613
TseI GCWGC 3 cut(s) 131, 149, 616
TspGWI ACGGA 1 cut(s) 452
TspRI CASTG 1 cut(s) 613
Van91I CCANNNNNTGG 1 cut(s) 160
VneI GTGCAC 1 cut(s) 297
VspI ATTAAT 1 cut(s) 585
XapI RAATTY 4 cut(s) 414, 425, 536, 702
XspI CTAG 2 cut(s) 8, 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.