MD17G1194500.v1.1

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014770
Physical Location & Seq
Reverse (-)
23303154 .. 23306481
3328 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1194500.v1.1.491

Sequence Viewer

Length: 603 bp
ATGGCGAGGCCTGGCAACAAGATCATACAAGCCAAACTGGTGCTTCTAGGGGACATGGGAACTGGGAAAACCAGCTTGGTGTTAAGATTTGTCAAAGGCCAGTTCTTCGATAACCAGGAACCAACGATAGGAGCAGCTTTTTTCACTCAAATACTGTCTTTAAGTGAAGCCACTGTGAAGTTTGATATATGGGACACCGCAGGACAAGAACGATACCATAGCTTGGCTCCTATGTACTATCGTGGTGCAGCAGCAGCTGTAGTCGTGTATGACATCTCAAGCACGGAGACATTTACTCGAGCCAAAAAATGGGTTCAAGAATTGCAGAGGCAAGGGAATTTAAACTTGGTGTTGGCATTGGTTGCAAATAAGTGCGACTTGGAGGCAAAAAGGGAGGTCGAGAGTGAGGAAGGGGAGCAATTTGCTCAAGAGAACGGGATGTTTTTCTTGGAAACATCAGCCAAAACCGCGCAGAACATTAACGAGCTTTTCCATGAAATAGCGAAGAGATTGGCAAAAGCTGCTCCCGCAAAGCCTGCCAGAGTGAATCTAAACAGCGAAACACGCGACAGAAGAAGAATGGGATTTTGCTGCTTGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 ko04144 ko04145 ko04962 ko05146 ko05152 map04014 map04144 map04145 map04962 map05146 map05152
Pfam Domains
Protein Families
Ras

Protein Analysis

201

Amino Acids

22.34

Weight (kDa)

8.76

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 10 - 169 5.4e-16 ADP-ribosylation factor family
Ras PF00071 12 - 171 8.2e-60 Ras family
Roc PF08477 12 - 126 9.5e-35 Ras of Complex, Roc, domain of DAPkinase
GTP_EFTU PF00009 38 - 171 9.1e-06 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014770)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g31240 FvH4_6g31240
malus_domestica MD09G1212500.v1.1 MD17G1194500.v1.1
prunus_persica Prupe.3G070500_v2.0.a1
pyrus_communis pycom09g12980 pycom17g20030
rosa_chinensis RchiOBHm_Chr2g0137961
rosa_laevigata RLG00000019694
rosa_multiflora Rmu_sc0002170.1_g000053
rosa_roxburghii Rroxscaffold_2G00106620
rosa_rugosa Rorug02G0348300 Rorug02G0348400 Rorug02G0348500
rosa_samantha Rh2AG394800 Rh2CG381400
rosa_wichuraiana Rw2G032290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 223, 309
AccII CGCG 2 cut(s) 470, 567
AciI CCGC 3 cut(s) 198, 468, 528
AcsI RAATTY 1 cut(s) 337
AfaI GTAC 1 cut(s) 236
AfiI CCNNNNNNNGG 3 cut(s) 128, 223, 309
AgsI TTSAA 1 cut(s) 317
AjnI CCWGG 2 cut(s) 10, 114
AjuI GAANNNNNNNTTGG 4 cut(s) 59, 91, 329, 361
AluBI AGCT 6 cut(s) 75, 137, 222, 257, 487, 521
AluI AGCT 6 cut(s) 75, 137, 222, 257, 487, 521
Alw26I GTCTC 1 cut(s) 281
AlwNI CAGNNNCTG 1 cut(s) 257
Ama87I CYCGRG 1 cut(s) 297
AoxI GGCC 2 cut(s) 8, 97
ApeKI GCWGC 6 cut(s) 134, 248, 251, 254, 521, 591
ApoI RAATTY 1 cut(s) 337
AspLEI GCGC 1 cut(s) 472
AvaI CYCGRG 1 cut(s) 297
BbvI GCAGC 6 cut(s) 146, 260, 263, 266, 508, 578
BciT130I CCWGG 2 cut(s) 12, 116
BcoDI GTCTC 1 cut(s) 281
BfaI CTAG 1 cut(s) 47
BfmI CTRYAG 1 cut(s) 258
BisI GCNGC 6 cut(s) 135, 249, 252, 255, 522, 592
BlsI GCNGC 6 cut(s) 136, 250, 253, 256, 523, 593
Bme1390I CCNGG 2 cut(s) 12, 116
BmeT110I CYCGRG 1 cut(s) 297
BmiI GGNNCC 2 cut(s) 120, 228
BmrFI CCNGG 2 cut(s) 12, 116
BmrI ACTGGG 1 cut(s) 72
BmuI ACTGGG 1 cut(s) 72
BpuEI CTTGAG 2 cut(s) 262, 411
Bsc4I CCNNNNNNNGG 3 cut(s) 128, 223, 309
Bse1I ACTGG 3 cut(s) 42, 67, 100
BseBI CCWGG 2 cut(s) 12, 116
BseGI GGATG 1 cut(s) 444
BseLI CCNNNNNNNGG 3 cut(s) 128, 223, 309
BseNI ACTGG 3 cut(s) 42, 67, 100
BseXI GCAGC 6 cut(s) 146, 260, 263, 266, 508, 578
BsgI GTGCAG 1 cut(s) 267
Bsh1236I CGCG 2 cut(s) 470, 567
BshFI GGCC 2 cut(s) 10, 99
BsiHKCI CYCGRG 1 cut(s) 297
BslFI GGGAC 2 cut(s) 65, 206
BslI CCNNNNNNNGG 3 cut(s) 128, 223, 309
BsmAI GTCTC 1 cut(s) 281
BsmFI GGGAC 2 cut(s) 65, 206
BsnI GGCC 2 cut(s) 10, 99
BsoBI CYCGRG 1 cut(s) 297
Bsp143I GATC 1 cut(s) 21
BspACI CCGC 3 cut(s) 198, 468, 528
BspANI GGCC 2 cut(s) 10, 99
BspFNI CGCG 2 cut(s) 470, 567
BspLI GGNNCC 2 cut(s) 120, 228
BsrI ACTGG 3 cut(s) 42, 67, 100
BssMI GATC 1 cut(s) 21
Bst2UI CCWGG 2 cut(s) 12, 116
Bst4CI ACNGT 2 cut(s) 156, 175
Bst6I CTCTTC 1 cut(s) 500
BstAPI GCANNNNNTGC 3 cut(s) 362, 521, 536
BstC8I GCNNGC 1 cut(s) 537
BstF5I GGATG 1 cut(s) 444
BstFNI CGCG 2 cut(s) 470, 567
BstHHI GCGC 1 cut(s) 472
BstKTI GATC 1 cut(s) 24
BstMAI GTCTC 1 cut(s) 281
BstMBI GATC 1 cut(s) 21
BstMWI GCNNNNNNNGC 7 cut(s) 254, 362, 467, 521, 527, 536, 564
BstNI CCWGG 2 cut(s) 12, 116
BstSCI CCNGG 2 cut(s) 10, 114
BstSFI CTRYAG 1 cut(s) 258
BstUI CGCG 2 cut(s) 470, 567
BstV1I GCAGC 6 cut(s) 146, 260, 263, 266, 508, 578
BsuRI GGCC 2 cut(s) 10, 99
BtsCI GGATG 1 cut(s) 444
BtsIMutI CAGTG 1 cut(s) 171
Cac8I GCNNGC 1 cut(s) 537
CaiI CAGNNNCTG 1 cut(s) 257
CfoI GCGC 1 cut(s) 472
Csp6I GTAC 1 cut(s) 235
CviAII CATG 2 cut(s) 55, 494
CviQI GTAC 1 cut(s) 235
DpnI GATC 1 cut(s) 23
DpnII GATC 1 cut(s) 21
DraI TTTAAA 1 cut(s) 342
Eam1104I CTCTTC 1 cut(s) 500
EarI CTCTTC 1 cut(s) 500
Eco147I AGGCCT 1 cut(s) 10
Eco88I CYCGRG 1 cut(s) 297
EcoRII CCWGG 2 cut(s) 10, 114
FaeI CATG 2 cut(s) 58, 497
FaiI YATR 8 cut(s) 26, 56, 188, 190, 219, 233, 270, 495
FalI AAGNNNNNCTT 2 cut(s) 362, 394
FaqI GGGAC 2 cut(s) 65, 206
FatI CATG 2 cut(s) 54, 493
FauI CCCGC 1 cut(s) 535
Fnu4HI GCNGC 6 cut(s) 135, 249, 252, 255, 522, 592
FokI GGATG 1 cut(s) 451
Fsp4HI GCNGC 6 cut(s) 135, 249, 252, 255, 522, 592
FspBI CTAG 1 cut(s) 47
GlaI GCGC 1 cut(s) 471
GluI GCNGC 6 cut(s) 135, 249, 252, 255, 522, 592
HaeIII GGCC 2 cut(s) 10, 99
HhaI GCGC 1 cut(s) 472
Hin1II CATG 2 cut(s) 58, 497
Hin6I GCGC 1 cut(s) 470
HinP1I GCGC 1 cut(s) 470
HinfI GANTC 1 cut(s) 547
Hpy188III TCNNGA 3 cut(s) 317, 400, 428
HpyAV CCTTC 1 cut(s) 404
HpyCH4III ACNGT 2 cut(s) 156, 175
HpyCH4V TGCA 3 cut(s) 248, 325, 365
HpyF10VI GCNNNNNNNGC 7 cut(s) 254, 362, 467, 521, 527, 536, 564
Hsp92II CATG 2 cut(s) 58, 497
HspAI GCGC 1 cut(s) 470
Kzo9I GATC 1 cut(s) 21
LmnI GCTCC 4 cut(s) 131, 232, 415, 529
Lsp1109I GCAGC 6 cut(s) 146, 260, 263, 266, 508, 578
MaeI CTAG 1 cut(s) 47
MalI GATC 1 cut(s) 23
MboI GATC 1 cut(s) 21
MboII GAAGA 4 cut(s) 97, 517, 585, 588
MluCI AATT 3 cut(s) 320, 337, 419
MnlI CCTC 4 cut(s) 321, 376, 388, 400
MseI TTAA 4 cut(s) 83, 161, 341, 480
MspA1I CMGCKG 1 cut(s) 257
MspR9I CCNGG 2 cut(s) 12, 116
MvaI CCWGG 2 cut(s) 12, 116
MvnI CGCG 2 cut(s) 470, 567
MwoI GCNNNNNNNGC 7 cut(s) 254, 362, 467, 521, 527, 536, 564
NdeII GATC 1 cut(s) 21
NlaIII CATG 2 cut(s) 58, 497
NlaIV GGNNCC 2 cut(s) 120, 228
PaeR7I CTCGAG 1 cut(s) 297
PceI AGGCCT 1 cut(s) 10
PfeI GAWTC 1 cut(s) 547
PflMI CCANNNNNTGG 2 cut(s) 223, 309
PkrI GCNGC 6 cut(s) 136, 250, 253, 256, 523, 593
Psp6I CCWGG 2 cut(s) 10, 114
PspGI CCWGG 2 cut(s) 10, 114
PspN4I GGNNCC 2 cut(s) 120, 228
PspXI VCTCGAGB 1 cut(s) 297
PstNI CAGNNNCTG 1 cut(s) 257
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
SaqAI TTAA 4 cut(s) 83, 161, 341, 480
SatI GCNGC 6 cut(s) 135, 249, 252, 255, 522, 592
Sau3AI GATC 1 cut(s) 21
ScrFI CCNGG 2 cut(s) 12, 116
SetI ASST 7 cut(s) 77, 139, 224, 259, 399, 489, 523
SfcI CTRYAG 1 cut(s) 258
Sfr274I CTCGAG 1 cut(s) 297
SlaI CTCGAG 1 cut(s) 297
SmlI CTYRAG 3 cut(s) 277, 297, 426
SmoI CTYRAG 3 cut(s) 277, 297, 426
Sse9I AATT 3 cut(s) 320, 337, 419
SseBI AGGCCT 1 cut(s) 10
SsiI CCGC 3 cut(s) 198, 468, 528
SspMI CTAG 1 cut(s) 47
StuI AGGCCT 1 cut(s) 10
StyD4I CCNGG 2 cut(s) 10, 114
TaaI ACNGT 2 cut(s) 156, 175
TaqI TCGA 3 cut(s) 108, 298, 399
TasI AATT 3 cut(s) 320, 337, 419
TatI WGTACW 1 cut(s) 234
TfiI GAWTC 1 cut(s) 547
Tru1I TTAA 4 cut(s) 83, 161, 341, 480
Tru9I TTAA 4 cut(s) 83, 161, 341, 480
TscAI CASTG 1 cut(s) 178
TseI GCWGC 6 cut(s) 134, 248, 251, 254, 521, 591
TspDTI ATGAA 1 cut(s) 510
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 1 cut(s) 178
Van91I CCANNNNNTGG 2 cut(s) 223, 309
XapI RAATTY 1 cut(s) 337
XhoI CTCGAG 1 cut(s) 297
XspI CTAG 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.