Rroxscaffold_2G00106620

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014770
Physical Location & Seq
Reverse (-)
30208952 .. 30212083
3132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00106620.1

Sequence Viewer

Length: 552 bp
ATGCTTTTCTTACAGGTGCTTCTAGGAGACATGGGAACTGGGAAAACAAGTTTGGTACTTAGATTTGTCAAAGGCCAGTTTTTCGATCACCAGGAGCCAACCATAGGAGCAGCTTTTTTCACTCAGATACTATCTTTAAGTGAAGCCACTGTGAAATTTGATATATGGGACACAGCAGGACAAGAACGATACCATAGCTTGGCTCCTATGTACTATCGCGGTGCAGCAGCAGCTGTAGTCGTCTATGACATCTCAAGCACGGATACATTCACTCGAGCCAAAAAATGGGTTCAAGAATTGCAAAGGCAAGGGAATTTAAATTTGGTGATGGCATTGGTTGCAAATAAGTCTGACTTGGAGGCAAAAAGGGAGGTGGACAATGAGGAAGGGGAGCAATTTGCCCAAGATAATGGGATGTTTTACATGGAAACTTCAGCCAAAACCTCGCAGAATATTAATGAACTTTTCCACGAAATAGACCTCAGACTTCTGAATATCCATGTTGCCTTGACTATCCAAGCCAAGGTGACTGATGTTAAAGAAAGCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 ko04144 ko04145 ko04962 ko05146 ko05152 map04014 map04144 map04145 map04962 map05146 map05152
Pfam Domains
Protein Families
Ras

Protein Analysis

183

Amino Acids

20.65

Weight (kDa)

5.08

Isoelectric Point (pI)

38.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ras PF00071 6 - 159 1.9e-57 Ras family
Roc PF08477 6 - 118 2.7e-33 Ras of Complex, Roc, domain of DAPkinase
Arf PF00025 6 - 158 1.4e-14 ADP-ribosylation factor family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014770)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g31240 FvH4_6g31240
malus_domestica MD09G1212500.v1.1 MD17G1194500.v1.1
prunus_persica Prupe.3G070500_v2.0.a1
pyrus_communis pycom09g12980 pycom17g20030
rosa_chinensis RchiOBHm_Chr2g0137961
rosa_laevigata RLG00000019694
rosa_multiflora Rmu_sc0002170.1_g000053
rosa_roxburghii Rroxscaffold_2G00106620
rosa_rugosa Rorug02G0348300 Rorug02G0348400 Rorug02G0348500
rosa_samantha Rh2AG394800 Rh2CG381400
rosa_wichuraiana Rw2G032290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 199, 285
AccII CGCG 1 cut(s) 219
AciI CCGC 1 cut(s) 219
AcsI RAATTY 3 cut(s) 155, 313, 319
AcuI CTGAAG 1 cut(s) 417
AfaI GTAC 2 cut(s) 57, 212
AfiI CCNNNNNNNGG 4 cut(s) 104, 199, 285, 523
AgsI TTSAA 1 cut(s) 293
AjnI CCWGG 1 cut(s) 90
AjuI GAANNNNNNNTTGG 4 cut(s) 35, 67, 305, 337
AluBI AGCT 3 cut(s) 113, 198, 233
AluI AGCT 3 cut(s) 113, 198, 233
Alw26I GTCTC 1 cut(s) 21
AlwNI CAGNNNCTG 1 cut(s) 233
Ama87I CYCGRG 1 cut(s) 273
AoxI GGCC 1 cut(s) 73
ApeKI GCWGC 4 cut(s) 110, 224, 227, 230
ApoI RAATTY 3 cut(s) 155, 313, 319
AseI ATTAAT 1 cut(s) 456
AsuHPI GGTGA 3 cut(s) 80, 337, 538
AvaI CYCGRG 1 cut(s) 273
BbvI GCAGC 4 cut(s) 122, 236, 239, 242
BccI CCATC 1 cut(s) 322
BciT130I CCWGG 1 cut(s) 92
BciVI GTATCC 1 cut(s) 256
BcoDI GTCTC 1 cut(s) 21
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 234
BfuI GTATCC 1 cut(s) 256
BisI GCNGC 4 cut(s) 111, 225, 228, 231
BlsI GCNGC 4 cut(s) 112, 226, 229, 232
Bme1390I CCNGG 1 cut(s) 92
BmeT110I CYCGRG 1 cut(s) 273
BmiI GGNNCC 2 cut(s) 96, 204
BmrFI CCNGG 1 cut(s) 92
BmrI ACTGGG 1 cut(s) 48
BmuI ACTGGG 1 cut(s) 48
BpuEI CTTGAG 1 cut(s) 238
BsaJI CCNNGG 1 cut(s) 522
Bsc4I CCNNNNNNNGG 4 cut(s) 104, 199, 285, 523
Bse1I ACTGG 2 cut(s) 43, 76
BseBI CCWGG 1 cut(s) 92
BseDI CCNNGG 1 cut(s) 522
BseGI GGATG 1 cut(s) 420
BseLI CCNNNNNNNGG 4 cut(s) 104, 199, 285, 523
BseMII CTCAG 2 cut(s) 137, 496
BseNI ACTGG 2 cut(s) 43, 76
BseXI GCAGC 4 cut(s) 122, 236, 239, 242
BsgI GTGCAG 1 cut(s) 243
Bsh1236I CGCG 1 cut(s) 219
BshFI GGCC 1 cut(s) 75
BsiHKCI CYCGRG 1 cut(s) 273
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 4 cut(s) 104, 199, 285, 523
BsmAI GTCTC 1 cut(s) 21
BsmFI GGGAC 1 cut(s) 182
BsnI GGCC 1 cut(s) 75
BsoBI CYCGRG 1 cut(s) 273
Bsp143I GATC 1 cut(s) 85
BspACI CCGC 1 cut(s) 219
BspANI GGCC 1 cut(s) 75
BspCNI CTCAG 2 cut(s) 136, 495
BspFNI CGCG 1 cut(s) 219
BspLI GGNNCC 2 cut(s) 96, 204
BsrI ACTGG 2 cut(s) 43, 76
BssECI CCNNGG 1 cut(s) 522
BssMI GATC 1 cut(s) 85
BssT1I CCWWGG 1 cut(s) 522
Bst2UI CCWGG 1 cut(s) 92
Bst4CI ACNGT 1 cut(s) 151
BstAPI GCANNNNNTGC 1 cut(s) 338
BstDEI CTNAG 3 cut(s) 59, 123, 482
BstF5I GGATG 1 cut(s) 420
BstFNI CGCG 1 cut(s) 219
BstKTI GATC 1 cut(s) 88
BstMAI GTCTC 1 cut(s) 21
BstMBI GATC 1 cut(s) 85
BstMWI GCNNNNNNNGC 2 cut(s) 230, 338
BstNI CCWGG 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 90
BstSFI CTRYAG 1 cut(s) 234
BstUI CGCG 1 cut(s) 219
BstV1I GCAGC 4 cut(s) 122, 236, 239, 242
BstXI CCANNNNNNTGG 1 cut(s) 410
BsuI GTATCC 1 cut(s) 256
BsuRI GGCC 1 cut(s) 75
BtsCI GGATG 1 cut(s) 420
BtsIMutI CAGTG 1 cut(s) 147
CaiI CAGNNNCTG 1 cut(s) 233
Csp6I GTAC 2 cut(s) 56, 211
CviAII CATG 3 cut(s) 31, 424, 500
CviQI GTAC 2 cut(s) 56, 211
DdeI CTNAG 3 cut(s) 59, 123, 482
DpnI GATC 1 cut(s) 87
DpnII GATC 1 cut(s) 85
DraI TTTAAA 1 cut(s) 318
Eco130I CCWWGG 1 cut(s) 522
Eco57I CTGAAG 1 cut(s) 417
Eco88I CYCGRG 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 90
EcoT14I CCWWGG 1 cut(s) 522
ErhI CCWWGG 1 cut(s) 522
FaeI CATG 3 cut(s) 34, 427, 503
FaiI YATR 9 cut(s) 32, 104, 164, 166, 195, 209, 246, 425, 501
FalI AAGNNNNNCTT 2 cut(s) 338, 370
FaqI GGGAC 1 cut(s) 182
FatI CATG 3 cut(s) 30, 423, 499
Fnu4HI GCNGC 4 cut(s) 111, 225, 228, 231
FokI GGATG 1 cut(s) 427
Fsp4HI GCNGC 4 cut(s) 111, 225, 228, 231
FspBI CTAG 1 cut(s) 23
GluI GCNGC 4 cut(s) 111, 225, 228, 231
HaeIII GGCC 1 cut(s) 75
Hin1II CATG 3 cut(s) 34, 427, 503
HphI GGTGA 3 cut(s) 80, 337, 538
Hpy166II GTNNAC 1 cut(s) 376
Hpy188I TCNGA 4 cut(s) 126, 352, 485, 492
Hpy188III TCNNGA 1 cut(s) 293
Hpy8I GTNNAC 1 cut(s) 376
HpyAV CCTTC 1 cut(s) 380
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4V TGCA 3 cut(s) 224, 301, 341
HpyF10VI GCNNNNNNNGC 2 cut(s) 230, 338
HpyF3I CTNAG 3 cut(s) 59, 123, 482
Hsp92II CATG 3 cut(s) 34, 427, 503
Kzo9I GATC 1 cut(s) 85
LmnI GCTCC 4 cut(s) 94, 107, 208, 391
LpnPI CCDG 5 cut(s) 24, 77, 89, 104, 162
Lsp1109I GCAGC 4 cut(s) 122, 236, 239, 242
MaeI CTAG 1 cut(s) 23
MaeIII GTNAC 1 cut(s) 526
MalI GATC 1 cut(s) 87
MboI GATC 1 cut(s) 85
MluCI AATT 5 cut(s) 155, 296, 313, 319, 395
MnlI CCTC 5 cut(s) 352, 364, 376, 454, 491
MseI TTAA 4 cut(s) 137, 317, 456, 537
MspA1I CMGCKG 1 cut(s) 233
MspR9I CCNGG 1 cut(s) 92
MvaI CCWGG 1 cut(s) 92
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 2 cut(s) 230, 338
NdeII GATC 1 cut(s) 85
NlaIII CATG 3 cut(s) 34, 427, 503
NlaIV GGNNCC 2 cut(s) 96, 204
NmuCI GTSAC 1 cut(s) 526
PaeR7I CTCGAG 1 cut(s) 273
PflMI CCANNNNNTGG 2 cut(s) 199, 285
PkrI GCNGC 4 cut(s) 112, 226, 229, 232
PshBI ATTAAT 1 cut(s) 456
Psp6I CCWGG 1 cut(s) 90
PspGI CCWGG 1 cut(s) 90
PspN4I GGNNCC 2 cut(s) 96, 204
PspXI VCTCGAGB 1 cut(s) 273
PstNI CAGNNNCTG 1 cut(s) 233
PvuII CAGCTG 1 cut(s) 233
RsaI GTAC 2 cut(s) 57, 212
RsaNI GTAC 2 cut(s) 56, 211
SaqAI TTAA 4 cut(s) 137, 317, 456, 537
SatI GCNGC 4 cut(s) 111, 225, 228, 231
Sau3AI GATC 1 cut(s) 85
ScrFI CCNGG 1 cut(s) 92
SetI ASST 8 cut(s) 18, 115, 200, 235, 375, 446, 483, 528
SfcI CTRYAG 1 cut(s) 234
Sfr274I CTCGAG 1 cut(s) 273
SlaI CTCGAG 1 cut(s) 273
SmiI ATTTAAAT 1 cut(s) 318
SmlI CTYRAG 2 cut(s) 253, 273
SmoI CTYRAG 2 cut(s) 253, 273
Sse9I AATT 5 cut(s) 155, 296, 313, 319, 395
SsiI CCGC 1 cut(s) 219
SspI AATATT 1 cut(s) 454
SspMI CTAG 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 90
StyI CCWWGG 1 cut(s) 522
SwaI ATTTAAAT 1 cut(s) 318
TaaI ACNGT 1 cut(s) 151
TaqI TCGA 2 cut(s) 84, 274
TasI AATT 5 cut(s) 155, 296, 313, 319, 395
TatI WGTACW 1 cut(s) 210
Tru1I TTAA 4 cut(s) 137, 317, 456, 537
Tru9I TTAA 4 cut(s) 137, 317, 456, 537
TscAI CASTG 1 cut(s) 154
TseFI GTSAC 1 cut(s) 526
TseI GCWGC 4 cut(s) 110, 224, 227, 230
Tsp45I GTSAC 1 cut(s) 526
TspDTI ATGAA 1 cut(s) 474
TspGWI ACGGA 1 cut(s) 275
TspRI CASTG 1 cut(s) 154
Van91I CCANNNNNTGG 2 cut(s) 199, 285
VspI ATTAAT 1 cut(s) 456
XapI RAATTY 3 cut(s) 155, 313, 319
XhoI CTCGAG 1 cut(s) 273
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.