Rorug02G0348500

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0014770
Physical Location & Seq
Reverse (-)
43692517 .. 43693674
1158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0348500.1

Sequence Viewer

Length: 1158 bp
ATGAGCATGGAATTCTCATCATGCTACCTTCCTGAAGAAATGATGATCGAAATTCTTCTGAGGCTACCCATCAAGTCTCTCCTTCAGTTCATCTCTGTTTCCAAATATTGGAACTCCCTTATTAAACACAATACCTTCATCAAAGACCACCTCAAACTCACTTCAAACAGAATCACCAGAGATGGCCCTTTGCTCCTTCTCAGGCATTGCCCCAGAGAGCCCAATGTAGAGCAGTATTCTCTACATTTAGACAACCACACCTTCCAAGAGTACTCAAAACCACAACTTCCAGCTAAGAGCTTCAATGAGTGTTTCCGAATTGTGGGTTCCTGCAATGGATTAATCCTCCTCTCAGACGATTACCTGACCGAGACCAATACCTTCATTCTATGGAACCCCTCAATTCGAAAGTCCATCACTCTTCCTAAACCTCATATCCGACACTCTCAATACTATACAGTTTATGGGTTTGGGTTTGATGCTAAGAAAGATGATTACAAGATAGTGCAGCTGGTGTATGAAGGTCACAACAGAGCTGGCCCTGAAGTTGAGCTCTACTCACTCAATTCAGGGTCTTGGAAAGCCATTACTGCAGCTTCTCCCAAGCATGAGATTGCTCCGGCAATGTGGTCTCAGGTTTTTGTTAATGGGTTTATCCATTTCATAGGGTATTCTAAGAAAGGAGAAGCTTTTCACAATGTTGTTTTGGGATTTGATGTGTGTGAAGAGGTTTTTCATGAGATTAAAATGCCAGAGGACTTGGCTTCTGAGGCGCCAAACATGTTCATTTCAGTCATTGGCAAGTCCCTTTCTGTGCAACACTCTGATATTATACATAACTGTTGGAATGTATGGGTTATGAGAGAGTATGGGGTGGTGGAGTCATGGACAAAGCAATTCACCATTGATATTCTCACCCCGAACTATCGGGTAACAAGGGTTTTGGGGTCTAGCAAGAATGGTGAGTTTCTTTTAGAAATGTATAAAGGAAAGAAGGGAGAGATAGTCCTTCATGATCCGAAGAAAAACACAAATGAGCATCTTGGAATTTATACAGATCCAGGGTACACAAGTCTTGAATATTACATGGAAAGTCTAGTTTTACTTGATAAAGCAACTTCATTTGCAGATTCCATGGAGCAAGAAGAGGAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 ko04144 ko04145 ko04962 ko05146 ko05152 map04014 map04144 map04145 map04962 map05146 map05152
Pfam Domains
Protein Families
Ras

Protein Analysis

385

Amino Acids

44.45

Weight (kDa)

5.9

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 9 - 44 2e-06 F-box-like
F-box PF00646 10 - 46 3.4e-08 F-box domain
FBA_1 PF07734 87 - 343 1.4e-27 F-box associated beta propeller domain
FBA_3 PF08268 100 - 346 6.3e-27 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014770)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g31240 FvH4_6g31240
malus_domestica MD09G1212500.v1.1 MD17G1194500.v1.1
prunus_persica Prupe.3G070500_v2.0.a1
pyrus_communis pycom09g12980 pycom17g20030
rosa_chinensis RchiOBHm_Chr2g0137961
rosa_laevigata RLG00000019694
rosa_multiflora Rmu_sc0002170.1_g000053
rosa_roxburghii Rroxscaffold_2G00106620
rosa_rugosa Rorug02G0348300 Rorug02G0348400 Rorug02G0348500
rosa_samantha Rh2AG394800 Rh2CG381400
rosa_wichuraiana Rw2G032290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 772
AccB7I CCANNNNNTGG 1 cut(s) 108
AclWI GGATC 2 cut(s) 1010, 1052
AcsI RAATTY 3 cut(s) 11, 51, 1047
AcuI CTGAAG 3 cut(s) 54, 68, 564
AcyI GRCGYC 1 cut(s) 773
AfaI GTAC 2 cut(s) 272, 1067
AfiI CCNNNNNNNGG 2 cut(s) 108, 322
AflIII ACRYGT 1 cut(s) 780
AgsI TTSAA 3 cut(s) 165, 304, 1079
AjnI CCWGG 1 cut(s) 1060
AluBI AGCT 7 cut(s) 293, 300, 511, 536, 553, 596, 689
AluI AGCT 7 cut(s) 293, 300, 511, 536, 553, 596, 689
Alw21I GWGCWC 1 cut(s) 555
Alw26I GTCTC 3 cut(s) 81, 365, 636
AlwI GGATC 2 cut(s) 1010, 1052
AoxI GGCC 2 cut(s) 184, 538
ApeKI GCWGC 2 cut(s) 508, 593
ApoI RAATTY 3 cut(s) 11, 51, 1047
AseI ATTAAT 1 cut(s) 341
Asp700I GAANNNNTTC 2 cut(s) 54, 690
AspLEI GCGC 1 cut(s) 775
AspS9I GGNCC 2 cut(s) 185, 539
AsuHPI GGTGA 4 cut(s) 166, 892, 907, 974
AsuII TTCGAA 1 cut(s) 406
BaeI ACNNNNGTAYC 2 cut(s) 1057, 1090
BanI GGYRCC 1 cut(s) 772
BanII GRGCYC 2 cut(s) 222, 555
Bbv12I GWGCWC 1 cut(s) 555
BbvI GCAGC 2 cut(s) 520, 605
BccI CCATC 3 cut(s) 77, 176, 422
BciT130I CCWGG 1 cut(s) 1062
BcoDI GTCTC 3 cut(s) 81, 365, 636
BfaI CTAG 2 cut(s) 951, 1097
BfmI CTRYAG 1 cut(s) 591
BfoI RGCGCY 1 cut(s) 776
BisI GCNGC 2 cut(s) 509, 594
BlsI GCNGC 2 cut(s) 510, 595
BmcAI AGTACT 1 cut(s) 272
Bme1390I CCNGG 1 cut(s) 1062
BmgT120I GGNCC 2 cut(s) 185, 539
BmiI GGNNCC 3 cut(s) 328, 395, 774
BmrFI CCNGG 1 cut(s) 1062
BmsI GCATC 2 cut(s) 469, 1048
BplI GAGNNNNNCTC 2 cut(s) 542, 574
Bpu14I TTCGAA 1 cut(s) 406
BsaHI GRCGYC 1 cut(s) 773
BsaI GGTCTC 2 cut(s) 365, 636
BsaJI CCNNGG 2 cut(s) 1061, 1134
BsaXI ACNNNNNCTCC 2 cut(s) 990, 1020
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 322
Bse3DI GCAATG 3 cut(s) 205, 340, 630
BseBI CCWGG 1 cut(s) 1062
BseDI CCNNGG 2 cut(s) 1061, 1134
BseLI CCNNNNNNNGG 2 cut(s) 108, 322
BseMI GCAATG 3 cut(s) 205, 340, 630
BseMII CTCAG 5 cut(s) 50, 214, 366, 647, 759
BseRI GAGGAG 1 cut(s) 338
BseXI GCAGC 2 cut(s) 520, 605
BsgI GTGCAG 1 cut(s) 527
BshFI GGCC 2 cut(s) 186, 540
BshNI GGYRCC 1 cut(s) 772
BsiHKAI GWGCWC 1 cut(s) 555
BsiSI CCGG 1 cut(s) 620
BslFI GGGAC 1 cut(s) 790
BslI CCNNNNNNNGG 2 cut(s) 108, 322
BsmAI GTCTC 3 cut(s) 81, 365, 636
BsmFI GGGAC 1 cut(s) 790
BsnI GGCC 2 cut(s) 186, 540
Bso31I GGTCTC 2 cut(s) 365, 636
Bsp119I TTCGAA 1 cut(s) 406
Bsp1286I GDGCHC 2 cut(s) 222, 555
Bsp143I GATC 3 cut(s) 45, 1015, 1057
Bsp19I CCATGG 1 cut(s) 1134
BspANI GGCC 2 cut(s) 186, 540
BspCNI CTCAG 5 cut(s) 51, 213, 365, 646, 760
BspHI TCATGA 2 cut(s) 736, 1012
BspLI GGNNCC 3 cut(s) 328, 395, 774
BspMAI CTGCAG 1 cut(s) 595
BspPI GGATC 2 cut(s) 1010, 1052
BspT104I TTCGAA 1 cut(s) 406
BspT107I GGYRCC 1 cut(s) 772
BspTNI GGTCTC 2 cut(s) 365, 636
BsrDI GCAATG 3 cut(s) 205, 340, 630
BssECI CCNNGG 2 cut(s) 1061, 1134
BssMI GATC 3 cut(s) 45, 1015, 1057
BssNI GRCGYC 1 cut(s) 773
BssT1I CCWWGG 1 cut(s) 1134
Bst2UI CCWGG 1 cut(s) 1062
Bst4CI ACNGT 2 cut(s) 460, 842
Bst6I CTCTTC 3 cut(s) 426, 720, 1140
BstACI GRCGYC 1 cut(s) 773
BstBI TTCGAA 1 cut(s) 406
BstC8I GCNNGC 1 cut(s) 538
BstDEI CTNAG 8 cut(s) 59, 200, 294, 352, 483, 633, 675, 768
BstDSI CCRYGG 1 cut(s) 1134
BstH2I RGCGCY 1 cut(s) 776
BstHHI GCGC 1 cut(s) 775
BstKTI GATC 3 cut(s) 48, 1018, 1060
BstMAI GTCTC 3 cut(s) 81, 365, 636
BstMBI GATC 3 cut(s) 45, 1015, 1057
BstMWI GCNNNNNNNGC 2 cut(s) 590, 770
BstNI CCWGG 1 cut(s) 1062
BstNSI RCATGY 1 cut(s) 784
BstSCI CCNGG 1 cut(s) 1060
BstSFI CTRYAG 1 cut(s) 591
BstV1I GCAGC 2 cut(s) 520, 605
BstX2I RGATCY 1 cut(s) 1057
BstYI RGATCY 1 cut(s) 1057
BsuRI GGCC 2 cut(s) 186, 540
BtgI CCRYGG 1 cut(s) 1134
Cac8I GCNNGC 1 cut(s) 538
CciI TCATGA 2 cut(s) 736, 1012
CfoI GCGC 1 cut(s) 775
Cfr13I GGNCC 2 cut(s) 185, 539
Csp6I GTAC 2 cut(s) 271, 1066
CviAII CATG 9 cut(s) 7, 21, 608, 737, 781, 885, 1013, 1087, 1135
CviQI GTAC 2 cut(s) 271, 1066
DdeI CTNAG 8 cut(s) 59, 200, 294, 352, 483, 633, 675, 768
DinI GGCGCC 1 cut(s) 774
DpnI GATC 3 cut(s) 47, 1017, 1059
DpnII GATC 3 cut(s) 45, 1015, 1057
Eam1104I CTCTTC 3 cut(s) 426, 720, 1140
EarI CTCTTC 3 cut(s) 426, 720, 1140
Ecl136II GAGCTC 1 cut(s) 553
Eco130I CCWWGG 1 cut(s) 1134
Eco24I GRGCYC 2 cut(s) 222, 555
Eco31I GGTCTC 2 cut(s) 365, 636
Eco53kI GAGCTC 1 cut(s) 553
Eco57I CTGAAG 3 cut(s) 54, 68, 564
EcoICRI GAGCTC 1 cut(s) 553
EcoRI GAATTC 1 cut(s) 11
EcoRII CCWGG 1 cut(s) 1060
EcoT14I CCWWGG 1 cut(s) 1134
EcoT38I GRGCYC 2 cut(s) 222, 555
EgeI GGCGCC 1 cut(s) 774
EheI GGCGCC 1 cut(s) 774
ErhI CCWWGG 1 cut(s) 1134
FaeI CATG 9 cut(s) 10, 24, 611, 740, 784, 888, 1016, 1090, 1138
FaqI GGGAC 1 cut(s) 790
FatI CATG 9 cut(s) 6, 20, 607, 736, 780, 884, 1012, 1086, 1134
Fnu4HI GCNGC 2 cut(s) 509, 594
FriOI GRGCYC 2 cut(s) 222, 555
Fsp4HI GCNGC 2 cut(s) 509, 594
FspBI CTAG 2 cut(s) 951, 1097
GlaI GCGC 1 cut(s) 774
GluI GCNGC 2 cut(s) 509, 594
HaeII RGCGCY 1 cut(s) 776
HaeIII GGCC 2 cut(s) 186, 540
HapII CCGG 1 cut(s) 620
HhaI GCGC 1 cut(s) 775
Hin1I GRCGYC 1 cut(s) 773
Hin1II CATG 9 cut(s) 10, 24, 611, 740, 784, 888, 1016, 1090, 1138
Hin6I GCGC 1 cut(s) 773
HinP1I GCGC 1 cut(s) 773
HindIII AAGCTT 1 cut(s) 687
HinfI GANTC 3 cut(s) 171, 881, 1130
HpaII CCGG 1 cut(s) 620
HphI GGTGA 4 cut(s) 166, 892, 907, 974
Hpy166II GTNNAC 1 cut(s) 1068
Hpy188I TCNGA 7 cut(s) 60, 317, 355, 440, 769, 826, 1020
Hpy188III TCNNGA 4 cut(s) 32, 737, 1013, 1076
Hpy8I GTNNAC 1 cut(s) 1068
HpyAV CCTTC 9 cut(s) 38, 92, 145, 206, 271, 391, 515, 988, 1019
HpyCH4III ACNGT 2 cut(s) 460, 842
HpyCH4V TGCA 5 cut(s) 333, 508, 593, 817, 1127
HpyF10VI GCNNNNNNNGC 2 cut(s) 590, 770
HpyF3I CTNAG 8 cut(s) 59, 200, 294, 352, 483, 633, 675, 768
Hsp92I GRCGYC 1 cut(s) 773
Hsp92II CATG 9 cut(s) 10, 24, 611, 740, 784, 888, 1016, 1090, 1138
HspAI GCGC 1 cut(s) 773
KasI GGCGCC 1 cut(s) 772
Kzo9I GATC 3 cut(s) 45, 1015, 1057
LmnI GCTCC 3 cut(s) 198, 622, 1138
Lsp1109I GCAGC 2 cut(s) 520, 605
LweI GCATC 2 cut(s) 469, 1048
MaeI CTAG 2 cut(s) 951, 1097
MaeIII GTNAC 2 cut(s) 524, 931
MalI GATC 3 cut(s) 47, 1017, 1059
MboI GATC 3 cut(s) 45, 1015, 1057
MboII GAAGA 6 cut(s) 47, 47, 413, 737, 1033, 1157
MflI RGATCY 1 cut(s) 1057
MhlI GDGCHC 2 cut(s) 222, 555
MluCI AATT 7 cut(s) 11, 51, 318, 402, 565, 896, 1047
Mly113I GGCGCC 1 cut(s) 773
MlyI GAGTC 1 cut(s) 890
MmeI TCCRAC 2 cut(s) 463, 824
MroXI GAANNNNTTC 2 cut(s) 54, 690
MseI TTAA 4 cut(s) 123, 341, 645, 744
MspA1I CMGCKG 1 cut(s) 511
MspI CCGG 1 cut(s) 620
MspR9I CCNGG 1 cut(s) 1062
MvaI CCWGG 1 cut(s) 1062
MwoI GCNNNNNNNGC 2 cut(s) 590, 770
NarI GGCGCC 1 cut(s) 773
NcoI CCATGG 1 cut(s) 1134
NdeII GATC 3 cut(s) 45, 1015, 1057
NlaIII CATG 9 cut(s) 10, 24, 611, 740, 784, 888, 1016, 1090, 1138
NlaIV GGNNCC 3 cut(s) 328, 395, 774
NmuCI GTSAC 1 cut(s) 524
NspI RCATGY 1 cut(s) 784
NspV TTCGAA 1 cut(s) 406
PagI TCATGA 2 cut(s) 736, 1012
PciI ACATGT 1 cut(s) 780
PdmI GAANNNNTTC 2 cut(s) 54, 690
PfeI GAWTC 2 cut(s) 171, 1130
PflMI CCANNNNNTGG 1 cut(s) 108
PkrI GCNGC 2 cut(s) 510, 595
PleI GAGTC 1 cut(s) 889
PluTI GGCGCC 1 cut(s) 776
PpsI GAGTC 1 cut(s) 889
PscI ACATGT 1 cut(s) 780
PshBI ATTAAT 1 cut(s) 341
Psp124BI GAGCTC 1 cut(s) 555
Psp6I CCWGG 1 cut(s) 1060
PspGI CCWGG 1 cut(s) 1060
PspN4I GGNNCC 3 cut(s) 328, 395, 774
PspPI GGNCC 2 cut(s) 185, 539
PstI CTGCAG 1 cut(s) 595
PsuI RGATCY 1 cut(s) 1057
PvuII CAGCTG 1 cut(s) 511
RsaI GTAC 2 cut(s) 272, 1067
RsaNI GTAC 2 cut(s) 271, 1066
SacI GAGCTC 1 cut(s) 555
SaqAI TTAA 4 cut(s) 123, 341, 645, 744
SatI GCNGC 2 cut(s) 509, 594
Sau3AI GATC 3 cut(s) 45, 1015, 1057
Sau96I GGNCC 2 cut(s) 185, 539
ScaI AGTACT 1 cut(s) 272
SchI GAGTC 1 cut(s) 890
ScrFI CCNGG 1 cut(s) 1062
SduI GDGCHC 2 cut(s) 222, 555
SfaNI GCATC 2 cut(s) 469, 1048
SfcI CTRYAG 1 cut(s) 591
SfoI GGCGCC 1 cut(s) 774
SfuI TTCGAA 1 cut(s) 406
Sse9I AATT 7 cut(s) 11, 51, 318, 402, 565, 896, 1047
SspDI GGCGCC 1 cut(s) 772
SspI AATATT 2 cut(s) 107, 1082
SspMI CTAG 2 cut(s) 951, 1097
SstI GAGCTC 1 cut(s) 555
StyD4I CCNGG 1 cut(s) 1060
StyI CCWWGG 1 cut(s) 1134
TaaI ACNGT 2 cut(s) 460, 842
TaqI TCGA 2 cut(s) 48, 406
TaqII GACCGA 1 cut(s) 383
TasI AATT 7 cut(s) 11, 51, 318, 402, 565, 896, 1047
TatI WGTACW 1 cut(s) 270
TfiI GAWTC 2 cut(s) 171, 1130
Tru1I TTAA 4 cut(s) 123, 341, 645, 744
Tru9I TTAA 4 cut(s) 123, 341, 645, 744
TseFI GTSAC 1 cut(s) 524
TseI GCWGC 2 cut(s) 508, 593
Tsp45I GTSAC 1 cut(s) 524
TspDTI ATGAA 9 cut(s) 79, 127, 373, 534, 652, 725, 775, 1001, 1110
Van91I CCANNNNNTGG 1 cut(s) 108
VspI ATTAAT 1 cut(s) 341
XapI RAATTY 3 cut(s) 11, 51, 1047
XceI RCATGY 1 cut(s) 784
XmnI GAANNNNTTC 2 cut(s) 54, 690
XspI CTAG 2 cut(s) 951, 1097
ZrmI AGTACT 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.