MD17G1212100.v1.1

post-illumination chlorophyll fluorescence increase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008492
Physical Location & Seq
Forward (+)
25921807 .. 25925886
4080 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1212100.v1.1.491

Sequence Viewer

Length: 849 bp
ATGGCTGCAGCAACAAAATCTTCGATCTTTGCGGCGTCCACACAACATCTTCCTACCATACCGTCCATTTTTGGTAGCAAAGCAAGCCCACCTGTTTCAAACACTCTTGCTAGCAGTTTTCGGGGTTCATCGTTGGCGAGGTGTCCTTCGATAAGGAAGAAAGTTGTGAAGGGCAATGCGAAAGTCAGTGCTGCTGCAACGACTTTTGCACCAACTCCTGTAGAGGAACCCAAAGAGTTCTCACTACCCTCATGGGCTATGTTTGAACTTGGGAGAGCTCCTGTGTATTGGAAAACCATGAACGGTCTTCCTCCTACCGCTGGAGAAAAGCTAAGGATTTTCTACAACCCAGCTGCAAATAAAATTGTCCCAAATGAAGAATATGGGATTGCATTTAATGGAGGTTTTAATCAGCCTATAATGTGTGGCGCTGAGCCAAGGGCAATGCTTAGTATTAGCCGAGGCAAAGCCGATTCCCCAATGTATTCCATCCAAATATGTATTCCCCGGCACGCCCTGAACTTAATCTTTTCGTTTACAAACGGAGTTGATTGGGATGGTCCTTACCGACTACAATTTCAAGTTCCCAACGGTTTGAAGAACAAACCGATTGAATTCTTTAACGAGGGACTAGCAGAAGAGTTGAGTAAAGACGGTGCGTGTGAGAGAGCAATCTTTCCTGATACGTTTGTTGTGGTAACAAGATGCAATATAATTGGGAATTTGACGGTTGAAGGAGGCGATCGTTGCAACCTTAATCTCGTACCAGGATGCACAGATCCCAGTTCACCCTCCTACGACCCGCTTGCCAATGTGGATGATGGATCATGCCCAATTGAGCTAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

283

Amino Acids

30.38

Weight (kDa)

6.74

Isoelectric Point (pI)

41.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ig_PIFI PF25419 86 - 215 4e-70 PIFI-like, immunoglobulin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 32, 318, 803
AclWI GGATC 2 cut(s) 773, 832
AcsI RAATTY 2 cut(s) 614, 721
AcyI GRCGYC 1 cut(s) 35
AfaI GTAC 1 cut(s) 765
AfiI CCNNNNNNNGG 1 cut(s) 320
AgsI TTSAA 6 cut(s) 99, 266, 581, 598, 614, 734
AjnI CCWGG 1 cut(s) 766
AjuI GAANNNNNNNTTGG 2 cut(s) 486, 518
AluBI AGCT 4 cut(s) 278, 331, 353, 841
AluI AGCT 4 cut(s) 278, 331, 353, 841
Alw21I GWGCWC 1 cut(s) 280
AlwI GGATC 2 cut(s) 773, 832
ApeKI GCWGC 5 cut(s) 5, 8, 191, 194, 353
ApoI RAATTY 2 cut(s) 614, 721
AspLEI GCGC 1 cut(s) 431
AspS9I GGNCC 1 cut(s) 560
AsuC2I CCSGG 1 cut(s) 508
AsuHPI GGTGA 1 cut(s) 780
AsuNHI GCTAGC 1 cut(s) 110
AvaII GGWCC 1 cut(s) 560
BaeI ACNNNNGTAYC 2 cut(s) 675, 708
BanII GRGCYC 1 cut(s) 280
BbsI GAAGAC 1 cut(s) 299
Bbv12I GWGCWC 1 cut(s) 280
BbvI GCAGC 4 cut(s) 20, 178, 181, 340
BccI CCATC 3 cut(s) 497, 551, 815
BcgI CGANNNNNNTGC 2 cut(s) 788, 822
BciT130I CCWGG 1 cut(s) 768
BcnI CCSGG 1 cut(s) 508
BfaI CTAG 3 cut(s) 111, 632, 842
BfmI CTRYAG 2 cut(s) 6, 219
BfoI RGCGCY 1 cut(s) 432
BisI GCNGC 6 cut(s) 6, 9, 33, 192, 195, 354
BlpI GCTNAGC 1 cut(s) 432
BlsI GCNGC 6 cut(s) 7, 10, 34, 193, 196, 355
Bme1390I CCNGG 2 cut(s) 508, 768
Bme18I GGWCC 1 cut(s) 560
BmgT120I GGNCC 1 cut(s) 560
BmiI GGNNCC 1 cut(s) 228
BmrFI CCNGG 2 cut(s) 508, 768
BmrI ACTGGG 1 cut(s) 777
BmsI GCATC 2 cut(s) 695, 761
BmtI GCTAGC 1 cut(s) 114
BmuI ACTGGG 1 cut(s) 777
BpiI GAAGAC 1 cut(s) 299
BpmI CTGGAG 1 cut(s) 342
Bpu10I CCTNAGC 1 cut(s) 332
Bpu1102I GCTNAGC 1 cut(s) 432
BpuMI CCSGG 1 cut(s) 508
BsaHI GRCGYC 1 cut(s) 35
BsaJI CCNNGG 3 cut(s) 437, 460, 506
Bsc4I CCNNNNNNNGG 1 cut(s) 320
Bse1I ACTGG 1 cut(s) 783
Bse3DI GCAATG 2 cut(s) 181, 450
BseBI CCWGG 1 cut(s) 768
BseDI CCNNGG 3 cut(s) 437, 460, 506
BseGI GGATG 4 cut(s) 489, 562, 776, 823
BseLI CCNNNNNNNGG 1 cut(s) 320
BseMI GCAATG 2 cut(s) 181, 450
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 1 cut(s) 783
BseXI GCAGC 4 cut(s) 20, 178, 181, 340
BseYI CCCAGC 1 cut(s) 349
Bsh1285I CGRYCG 1 cut(s) 745
BsiEI CGRYCG 1 cut(s) 745
BsiHKAI GWGCWC 1 cut(s) 280
BsiSI CCGG 1 cut(s) 508
BslFI GGGAC 2 cut(s) 353, 642
BslI CCNNNNNNNGG 1 cut(s) 320
BsmFI GGGAC 2 cut(s) 353, 642
Bsp1286I GDGCHC 1 cut(s) 280
Bsp143I GATC 4 cut(s) 24, 742, 778, 824
Bsp1720I GCTNAGC 1 cut(s) 432
BspACI CCGC 3 cut(s) 32, 318, 803
BspCNI CTCAG 1 cut(s) 424
BspLI GGNNCC 1 cut(s) 228
BspMAI CTGCAG 1 cut(s) 10
BspOI GCTAGC 1 cut(s) 114
BspPI GGATC 2 cut(s) 773, 832
BsrDI GCAATG 2 cut(s) 181, 450
BsrI ACTGG 1 cut(s) 783
BssECI CCNNGG 3 cut(s) 437, 460, 506
BssMI GATC 4 cut(s) 24, 742, 778, 824
BssNI GRCGYC 1 cut(s) 35
BssT1I CCWWGG 1 cut(s) 437
Bst2UI CCWGG 1 cut(s) 768
Bst4CI ACNGT 5 cut(s) 63, 305, 593, 656, 730
Bst6I CTCTTC 1 cut(s) 633
BstACI GRCGYC 1 cut(s) 35
BstC8I GCNNGC 4 cut(s) 85, 112, 513, 807
BstDEI CTNAG 3 cut(s) 332, 432, 449
BstF5I GGATG 4 cut(s) 489, 562, 776, 823
BstH2I RGCGCY 1 cut(s) 432
BstHHI GCGC 1 cut(s) 431
BstKTI GATC 4 cut(s) 27, 745, 781, 827
BstMBI GATC 4 cut(s) 24, 742, 778, 824
BstMCI CGRYCG 1 cut(s) 745
BstMWI GCNNNNNNNGC 2 cut(s) 84, 747
BstNI CCWGG 1 cut(s) 768
BstSCI CCNGG 2 cut(s) 506, 766
BstSFI CTRYAG 2 cut(s) 6, 219
BstV1I GCAGC 4 cut(s) 20, 178, 181, 340
BstV2I GAAGAC 1 cut(s) 299
BstX2I RGATCY 1 cut(s) 778
BstYI RGATCY 1 cut(s) 778
BtsCI GGATG 4 cut(s) 489, 562, 776, 823
BtsIMutI CAGTG 1 cut(s) 193
Cac8I GCNNGC 4 cut(s) 85, 112, 513, 807
CfoI GCGC 1 cut(s) 431
Cfr13I GGNCC 1 cut(s) 560
CseI GACGC 1 cut(s) 24
Csp6I GTAC 1 cut(s) 764
CviAII CATG 3 cut(s) 252, 298, 828
CviQI GTAC 1 cut(s) 764
DdeI CTNAG 3 cut(s) 332, 432, 449
DpnI GATC 4 cut(s) 26, 744, 780, 826
DpnII GATC 4 cut(s) 24, 742, 778, 824
Eam1104I CTCTTC 1 cut(s) 633
EarI CTCTTC 1 cut(s) 633
Ecl136II GAGCTC 1 cut(s) 278
Eco130I CCWWGG 1 cut(s) 437
Eco24I GRGCYC 1 cut(s) 280
Eco47I GGWCC 1 cut(s) 560
Eco53kI GAGCTC 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 278
EcoRI GAATTC 1 cut(s) 614
EcoRII CCWGG 1 cut(s) 766
EcoT14I CCWWGG 1 cut(s) 437
EcoT38I GRGCYC 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 437
FaeI CATG 3 cut(s) 255, 301, 831
FaiI YATR 9 cut(s) 59, 253, 260, 299, 384, 419, 499, 713, 829
FaqI GGGAC 2 cut(s) 353, 642
FatI CATG 3 cut(s) 251, 297, 827
FauI CCCGC 1 cut(s) 810
Fnu4HI GCNGC 6 cut(s) 6, 9, 33, 192, 195, 354
FokI GGATG 4 cut(s) 476, 569, 783, 830
FriOI GRGCYC 1 cut(s) 280
Fsp4HI GCNGC 6 cut(s) 6, 9, 33, 192, 195, 354
FspBI CTAG 3 cut(s) 111, 632, 842
GlaI GCGC 1 cut(s) 430
GluI GCNGC 6 cut(s) 6, 9, 33, 192, 195, 354
GsaI CCCAGC 1 cut(s) 353
GsuI CTGGAG 1 cut(s) 342
HaeII RGCGCY 1 cut(s) 432
HapII CCGG 1 cut(s) 508
HgaI GACGC 1 cut(s) 24
HhaI GCGC 1 cut(s) 431
Hin1I GRCGYC 1 cut(s) 35
Hin1II CATG 3 cut(s) 255, 301, 831
Hin6I GCGC 1 cut(s) 429
HinP1I GCGC 1 cut(s) 429
HinfI GANTC 1 cut(s) 473
HpaII CCGG 1 cut(s) 508
HphI GGTGA 1 cut(s) 780
Hpy166II GTNNAC 3 cut(s) 39, 537, 788
Hpy188III TCNNGA 1 cut(s) 680
Hpy8I GTNNAC 3 cut(s) 39, 537, 788
HpyAV CCTTC 3 cut(s) 156, 163, 728
HpyCH4III ACNGT 5 cut(s) 63, 305, 593, 656, 730
HpyCH4IV ACGT 1 cut(s) 686
HpyCH4V TGCA 8 cut(s) 8, 197, 209, 356, 392, 708, 750, 774
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 747
HpyF3I CTNAG 3 cut(s) 332, 432, 449
HpySE526I ACGT 1 cut(s) 686
Hsp92I GRCGYC 1 cut(s) 35
Hsp92II CATG 3 cut(s) 255, 301, 831
HspAI GCGC 1 cut(s) 429
Kzo9I GATC 4 cut(s) 24, 742, 778, 824
LmnI GCTCC 1 cut(s) 283
Lsp1109I GCAGC 4 cut(s) 20, 178, 181, 340
LweI GCATC 2 cut(s) 695, 761
MaeI CTAG 3 cut(s) 111, 632, 842
MaeII ACGT 1 cut(s) 686
MaeIII GTNAC 1 cut(s) 697
MalI GATC 4 cut(s) 26, 744, 780, 826
MboI GATC 4 cut(s) 24, 742, 778, 824
MboII GAAGA 7 cut(s) 12, 41, 169, 299, 389, 610, 650
MfeI CAATTG 1 cut(s) 834
MflI RGATCY 1 cut(s) 778
MhlI GDGCHC 1 cut(s) 280
MluCI AATT 6 cut(s) 363, 575, 614, 714, 721, 834
MnlI CCTC 9 cut(s) 132, 217, 259, 321, 395, 455, 619, 731, 802
MseI TTAA 5 cut(s) 396, 408, 524, 621, 756
MspA1I CMGCKG 2 cut(s) 320, 353
MspI CCGG 1 cut(s) 508
MspR9I CCNGG 2 cut(s) 508, 768
MunI CAATTG 1 cut(s) 834
MvaI CCWGG 1 cut(s) 768
MwoI GCNNNNNNNGC 2 cut(s) 84, 747
NciI CCSGG 1 cut(s) 508
NdeII GATC 4 cut(s) 24, 742, 778, 824
NheI GCTAGC 1 cut(s) 110
NlaIII CATG 3 cut(s) 255, 301, 831
NlaIV GGNNCC 1 cut(s) 228
NmeAIII GCCGAG 1 cut(s) 485
PfeI GAWTC 1 cut(s) 473
PkrI GCNGC 6 cut(s) 7, 10, 34, 193, 196, 355
Ple19I CGATCG 1 cut(s) 745
Psp124BI GAGCTC 1 cut(s) 280
Psp6I CCWGG 1 cut(s) 766
PspFI CCCAGC 1 cut(s) 349
PspGI CCWGG 1 cut(s) 766
PspN4I GGNNCC 1 cut(s) 228
PspPI GGNCC 1 cut(s) 560
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 1 cut(s) 778
PvuI CGATCG 1 cut(s) 745
PvuII CAGCTG 1 cut(s) 353
RsaI GTAC 1 cut(s) 765
RsaNI GTAC 1 cut(s) 764
SacI GAGCTC 1 cut(s) 280
SaqAI TTAA 5 cut(s) 396, 408, 524, 621, 756
SatI GCNGC 6 cut(s) 6, 9, 33, 192, 195, 354
Sau3AI GATC 4 cut(s) 24, 742, 778, 824
Sau96I GGNCC 1 cut(s) 560
ScrFI CCNGG 2 cut(s) 508, 768
SduI GDGCHC 1 cut(s) 280
SetI ASST 9 cut(s) 94, 143, 280, 333, 355, 406, 689, 756, 843
SfaNI GCATC 2 cut(s) 695, 761
SfcI CTRYAG 2 cut(s) 6, 219
SinI GGWCC 1 cut(s) 560
Sse9I AATT 6 cut(s) 363, 575, 614, 714, 721, 834
SsiI CCGC 3 cut(s) 32, 318, 803
SspMI CTAG 3 cut(s) 111, 632, 842
SstI GAGCTC 1 cut(s) 280
StyD4I CCNGG 2 cut(s) 506, 766
StyI CCWWGG 1 cut(s) 437
TaaI ACNGT 5 cut(s) 63, 305, 593, 656, 730
TaiI ACGT 1 cut(s) 689
TaqI TCGA 2 cut(s) 23, 149
TasI AATT 6 cut(s) 363, 575, 614, 714, 721, 834
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 1 cut(s) 473
Tru1I TTAA 5 cut(s) 396, 408, 524, 621, 756
Tru9I TTAA 5 cut(s) 396, 408, 524, 621, 756
TscAI CASTG 1 cut(s) 193
TseI GCWGC 5 cut(s) 5, 8, 191, 194, 353
TspDTI ATGAA 3 cut(s) 117, 314, 390
TspGWI ACGGA 1 cut(s) 558
TspRI CASTG 1 cut(s) 193
VpaK11BI GGWCC 1 cut(s) 560
XapI RAATTY 2 cut(s) 614, 721
XspI CTAG 3 cut(s) 111, 632, 842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.