Rroxscaffold_2G00109270

post-illumination chlorophyll fluorescence increase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008492
Physical Location & Seq
Forward (+)
33416366 .. 33420412
4047 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00109270.1

Sequence Viewer

Length: 732 bp
ATGGCAGCAACAAATGGGTCAATCTTTGCGTCTTCCACTAAACCATTTCAGACCACACCGTCCATTCTCAGCAGCAAAGCAAGTCCAGCTTCAAACACACTTGGTAGCAGTTTTAAGGGTTCTTCATTGCCAAAGTGTCCTTTAAAATGGAAGAAAGTTGTCAAAAGCAGTGGAAAAGTTTGTGCCGCAGCAACAGGTGTTGCAACAACTCCAGTAGAGGAAACCAAAGAGTTAACACTTCCCTCATGGGCCATGTTTGAACTTGGAAGGGCCCCTGTATATTGGAAAACCATGAACGGTCTTCCTCCTACGGCTGGAGAAAAGCTAAGGATTTTCTACAATCCAGCTGCAAAGAATCTTGTTCCAAATGAAGAATACGGGGTTGGCTTCAATGGAGGTTTTAATCAACCTATAATGTGCGGTGGTGAGCCAAGGGCAATGCTTAGAAAAATCGAGGCAAAGCTGATTCCCCAATATATTCCATCCAAATTTGCATTCCGAAGCATGGGACTAGCTGAAGAGCTGAGTAAAGATGGTGCTTGTGAAAGAGCAATCTTTCCTGATTCGAGCATTGTTGTGAATAGATGTACCATCATGGGAAATTTGACTGTTGAAGGAGGTGATCGTTGCAACCTTGATCTCAGACTGGGATGCACAGATCCCAGCTCACCTCTTTATGACCCGAATGCTAATGTAGACGACGGAACATGCCCAATGGATATGGATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

243

Amino Acids

25.97

Weight (kDa)

8.37

Isoelectric Point (pI)

43.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ig_PIFI PF25419 84 - 155 6.1e-36 PIFI-like, immunoglobulin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 58
AccI GTMKAC 1 cut(s) 696
AciI CCGC 2 cut(s) 186, 420
AclWI GGATC 1 cut(s) 653
AcsI RAATTY 2 cut(s) 488, 601
AcuI CTGAAG 1 cut(s) 537
AfaI GTAC 1 cut(s) 589
AfiI CCNNNNNNNGG 2 cut(s) 314, 505
AgsI TTSAA 4 cut(s) 93, 260, 391, 614
AjuI GAANNNNNNNTTGG 4 cut(s) 366, 398, 479, 511
AluBI AGCT 7 cut(s) 89, 325, 347, 463, 515, 523, 666
AluI AGCT 7 cut(s) 89, 325, 347, 463, 515, 523, 666
AlwI GGATC 1 cut(s) 653
AoxI GGCC 2 cut(s) 249, 270
ApaI GGGCCC 1 cut(s) 274
ApeKI GCWGC 4 cut(s) 5, 72, 188, 347
ApoI RAATTY 2 cut(s) 488, 601
AspS9I GGNCC 3 cut(s) 249, 270, 271
AsuHPI GGTGA 3 cut(s) 437, 632, 660
BaeGI GKGCMC 1 cut(s) 274
BanII GRGCYC 1 cut(s) 274
BbsI GAAGAC 2 cut(s) 24, 293
BbvI GCAGC 4 cut(s) 17, 84, 200, 334
BccI CCATC 3 cut(s) 490, 527, 599
BceAI ACGGC 1 cut(s) 327
BfaI CTAG 1 cut(s) 512
BisI GCNGC 5 cut(s) 6, 73, 186, 189, 348
BlsI GCNGC 5 cut(s) 7, 74, 187, 190, 349
BmgT120I GGNCC 3 cut(s) 249, 270, 271
BmiI GGNNCC 2 cut(s) 272, 273
BmrI ACTGGG 1 cut(s) 656
BmsI GCATC 1 cut(s) 641
BmuI ACTGGG 1 cut(s) 656
BpiI GAAGAC 2 cut(s) 24, 293
BpmI CTGGAG 2 cut(s) 195, 336
Bpu10I CCTNAGC 1 cut(s) 326
BsaJI CCNNGG 1 cut(s) 431
Bsc4I CCNNNNNNNGG 2 cut(s) 314, 505
Bse1I ACTGG 2 cut(s) 212, 651
Bse3DI GCAATG 2 cut(s) 125, 444
BseDI CCNNGG 1 cut(s) 431
BseGI GGATG 2 cut(s) 482, 656
BseLI CCNNNNNNNGG 2 cut(s) 314, 505
BseMI GCAATG 2 cut(s) 125, 444
BseMII CTCAG 3 cut(s) 82, 515, 655
BseNI ACTGG 2 cut(s) 212, 651
BseSI GKGCMC 1 cut(s) 274
BseXI GCAGC 4 cut(s) 17, 84, 200, 334
BseYI CCCAGC 1 cut(s) 662
BshFI GGCC 2 cut(s) 251, 272
BslFI GGGAC 1 cut(s) 522
BslI CCNNNNNNNGG 2 cut(s) 314, 505
BsmFI GGGAC 1 cut(s) 522
BsmI GAATGC 2 cut(s) 494, 691
BsnI GGCC 2 cut(s) 251, 272
Bsp120I GGGCCC 1 cut(s) 270
Bsp1286I GDGCHC 1 cut(s) 274
Bsp143I GATC 3 cut(s) 622, 637, 658
BspACI CCGC 2 cut(s) 186, 420
BspANI GGCC 2 cut(s) 251, 272
BspCNI CTCAG 3 cut(s) 81, 516, 654
BspLI GGNNCC 2 cut(s) 272, 273
BspPI GGATC 1 cut(s) 653
BspQI GCTCTTC 1 cut(s) 513
BsrDI GCAATG 2 cut(s) 125, 444
BsrI ACTGG 2 cut(s) 212, 651
BssECI CCNNGG 1 cut(s) 431
BssMI GATC 3 cut(s) 622, 637, 658
BssT1I CCWWGG 1 cut(s) 431
Bst4CI ACNGT 3 cut(s) 60, 299, 610
Bst6I CTCTTC 1 cut(s) 513
BstDEI CTNAG 6 cut(s) 68, 326, 443, 524, 641, 729
BstF5I GGATG 2 cut(s) 482, 656
BstKTI GATC 3 cut(s) 625, 640, 661
BstMBI GATC 3 cut(s) 622, 637, 658
BstMWI GCNNNNNNNGC 1 cut(s) 86
BstNSI RCATGY 1 cut(s) 711
BstSLI GKGCMC 1 cut(s) 274
BstV1I GCAGC 4 cut(s) 17, 84, 200, 334
BstV2I GAAGAC 2 cut(s) 24, 293
BstX2I RGATCY 1 cut(s) 658
BstYI RGATCY 1 cut(s) 658
BsuRI GGCC 2 cut(s) 251, 272
BtsCI GGATG 2 cut(s) 482, 656
BtsI GCAGTG 1 cut(s) 175
BtsIMutI CAGTG 1 cut(s) 175
Cfr13I GGNCC 3 cut(s) 249, 270, 271
CseI GACGC 1 cut(s) 18
Csp6I GTAC 1 cut(s) 588
CspCI CAANNNNNGTGG 2 cut(s) 151, 186
CviAII CATG 6 cut(s) 246, 253, 292, 505, 595, 708
CviQI GTAC 1 cut(s) 588
DdeI CTNAG 6 cut(s) 68, 326, 443, 524, 641, 729
DpnI GATC 3 cut(s) 624, 639, 660
DpnII GATC 3 cut(s) 622, 637, 658
DraI TTTAAA 1 cut(s) 144
DrdI GACNNNNNNGTC 1 cut(s) 58
DseDI GACNNNNNNGTC 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 513
EarI CTCTTC 1 cut(s) 513
Eco130I CCWWGG 1 cut(s) 431
Eco24I GRGCYC 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 537
EcoO109I RGGNCCY 2 cut(s) 270, 271
EcoT14I CCWWGG 1 cut(s) 431
EcoT38I GRGCYC 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 431
FaeI CATG 6 cut(s) 249, 256, 295, 508, 598, 711
FalI AAGNNNNNCTT 2 cut(s) 73, 105
FaqI GGGAC 1 cut(s) 522
FatI CATG 6 cut(s) 245, 252, 291, 504, 594, 707
FblI GTMKAC 1 cut(s) 696
Fnu4HI GCNGC 5 cut(s) 6, 73, 186, 189, 348
FokI GGATG 2 cut(s) 469, 663
FriOI GRGCYC 1 cut(s) 274
Fsp4HI GCNGC 5 cut(s) 6, 73, 186, 189, 348
FspBI CTAG 1 cut(s) 512
GluI GCNGC 5 cut(s) 6, 73, 186, 189, 348
GsaI CCCAGC 1 cut(s) 666
GsuI CTGGAG 2 cut(s) 195, 336
HaeIII GGCC 2 cut(s) 251, 272
HgaI GACGC 1 cut(s) 18
Hin1II CATG 6 cut(s) 249, 256, 295, 508, 598, 711
HincII GTYRAC 1 cut(s) 234
HindII GTYRAC 1 cut(s) 234
HinfI GANTC 4 cut(s) 355, 466, 563, 725
HpaI GTTAAC 1 cut(s) 234
HphI GGTGA 3 cut(s) 437, 632, 660
Hpy166II GTNNAC 2 cut(s) 234, 697
Hpy188I TCNGA 3 cut(s) 51, 500, 644
Hpy188III TCNNGA 1 cut(s) 560
Hpy8I GTNNAC 2 cut(s) 234, 697
Hpy99I CGWCG 1 cut(s) 704
HpyAV CCTTC 2 cut(s) 261, 608
HpyCH4III ACNGT 3 cut(s) 60, 299, 610
HpyCH4V TGCA 5 cut(s) 203, 350, 494, 630, 654
HpyF10VI GCNNNNNNNGC 1 cut(s) 86
HpyF3I CTNAG 6 cut(s) 68, 326, 443, 524, 641, 729
Hsp92II CATG 6 cut(s) 249, 256, 295, 508, 598, 711
KspAI GTTAAC 1 cut(s) 234
Kzo9I GATC 3 cut(s) 622, 637, 658
LguI GCTCTTC 1 cut(s) 513
LpnPI CCDG 9 cut(s) 99, 180, 225, 288, 300, 357, 573, 632, 676
Lsp1109I GCAGC 4 cut(s) 17, 84, 200, 334
LweI GCATC 1 cut(s) 641
MaeI CTAG 1 cut(s) 512
MalI GATC 3 cut(s) 624, 639, 660
MboI GATC 3 cut(s) 622, 637, 658
MboII GAAGA 6 cut(s) 24, 114, 163, 293, 383, 530
MflI RGATCY 1 cut(s) 658
MhlI GDGCHC 1 cut(s) 274
MluCI AATT 2 cut(s) 488, 601
MnlI CCTC 7 cut(s) 211, 253, 315, 389, 448, 611, 681
MseI TTAA 4 cut(s) 114, 143, 233, 402
MslI CAYNNNNRTG 1 cut(s) 575
MspA1I CMGCKG 1 cut(s) 347
Mva1269I GAATGC 2 cut(s) 494, 691
MwoI GCNNNNNNNGC 1 cut(s) 86
NdeII GATC 3 cut(s) 622, 637, 658
NlaIII CATG 6 cut(s) 249, 256, 295, 508, 598, 711
NlaIV GGNNCC 2 cut(s) 272, 273
NspI RCATGY 1 cut(s) 711
PciSI GCTCTTC 1 cut(s) 513
PctI GAATGC 2 cut(s) 494, 691
PfeI GAWTC 4 cut(s) 355, 466, 563, 725
PkrI GCNGC 5 cut(s) 7, 74, 187, 190, 349
PspFI CCCAGC 1 cut(s) 662
PspN4I GGNNCC 2 cut(s) 272, 273
PspOMI GGGCCC 1 cut(s) 270
PspPI GGNCC 3 cut(s) 249, 270, 271
PsuI RGATCY 1 cut(s) 658
PvuII CAGCTG 1 cut(s) 347
RsaI GTAC 1 cut(s) 589
RsaNI GTAC 1 cut(s) 588
RseI CAYNNNNRTG 1 cut(s) 575
SapI GCTCTTC 1 cut(s) 513
SaqAI TTAA 4 cut(s) 114, 143, 233, 402
SatI GCNGC 5 cut(s) 6, 73, 186, 189, 348
Sau3AI GATC 3 cut(s) 622, 637, 658
Sau96I GGNCC 3 cut(s) 249, 270, 271
SduI GDGCHC 1 cut(s) 274
SfaNI GCATC 1 cut(s) 641
SmiMI CAYNNNNRTG 1 cut(s) 575
Sse9I AATT 2 cut(s) 488, 601
SsiI CCGC 2 cut(s) 186, 420
SspMI CTAG 1 cut(s) 512
StyI CCWWGG 1 cut(s) 431
TaaI ACNGT 3 cut(s) 60, 299, 610
TaqI TCGA 2 cut(s) 453, 566
TasI AATT 2 cut(s) 488, 601
TauI GCSGC 1 cut(s) 188
TfiI GAWTC 4 cut(s) 355, 466, 563, 725
Tru1I TTAA 4 cut(s) 114, 143, 233, 402
Tru9I TTAA 4 cut(s) 114, 143, 233, 402
TscAI CASTG 1 cut(s) 175
TseI GCWGC 4 cut(s) 5, 72, 188, 347
TspDTI ATGAA 3 cut(s) 114, 308, 384
TspGWI ACGGA 1 cut(s) 717
TspRI CASTG 1 cut(s) 175
XapI RAATTY 2 cut(s) 488, 601
XceI RCATGY 1 cut(s) 711
XmiI GTMKAC 1 cut(s) 696
XspI CTAG 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.