Rh2AG370300

post-illumination chlorophyll fluorescence increase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0008492
Physical Location & Seq
Forward (+)
54983144 .. 54987316
4173 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG370300.1

Sequence Viewer

Length: 594 bp
ATGTTTGAACTTGGAAGGGCCCCTGTATATTGGAAAACCATGAACGGTCTTCCTCCTACGGCTGGAGAAAAGCTAAGGATTTTCTACAATCCAGCTGCAAAGAATCTTGTTCCAAATGAAGAATACGGGGTTGCCTTCAATGGAGGTTTTAATCAACCTATAATGTGTGGTGGTGAGCCAAGGGCCATGCTTAGAAAAAATCGAGGCAAAGCTGATTCCCCAATATATTCCATCCAAATTTGCATTCCGAAGCATGCTCTGAACTTAATTTTTTCATTTACAAATGGAGATGAATGGGATGGTCCTTACAGATTACAATTTCAAGTTCCCAAGGGTTTCAAAAACAAACCAATTGAATTCTTCAACGAGGGACTAGCCGAAGAGCTGAGTAAAGATGGTGCTTGCGAAAGAGCAATCTTTCCGGATTCGAGCATTGTTGTGAATAGATGTACCATCATGGGAAATTTGACCGTTGAAGGAGGTGATCGTTGCAACCTTGATCTCAGACTGGGATGCACGGATCCCAGCTCACCTCTCTATGACCCGAATGCCAATGTAGACGACGGAACGTGCCCAATGGATCTGGATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

21.79

Weight (kDa)

4.98

Isoelectric Point (pI)

37.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ig_PIFI PF25419 1 - 129 1.6e-73 PIFI-like, immunoglobulin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 558
AccIII TCCGGA 1 cut(s) 421
AclWI GGATC 3 cut(s) 515, 528, 588
AcsI RAATTY 3 cut(s) 237, 356, 463
AfaI GTAC 1 cut(s) 451
AfiI CCNNNNNNNGG 1 cut(s) 62
AgsI TTSAA 7 cut(s) 8, 139, 323, 340, 356, 364, 476
AjuI GAANNNNNNNTTGG 2 cut(s) 228, 260
AluBI AGCT 5 cut(s) 73, 95, 212, 385, 528
AluI AGCT 5 cut(s) 73, 95, 212, 385, 528
AlwI GGATC 3 cut(s) 515, 528, 588
Aor13HI TCCGGA 1 cut(s) 421
AoxI GGCC 2 cut(s) 18, 183
ApaI GGGCCC 1 cut(s) 22
ApeKI GCWGC 1 cut(s) 95
ApoI RAATTY 3 cut(s) 237, 356, 463
AspS9I GGNCC 4 cut(s) 18, 19, 183, 302
AsuHPI GGTGA 3 cut(s) 185, 494, 522
AvaII GGWCC 1 cut(s) 302
BaeGI GKGCMC 2 cut(s) 22, 575
BamHI GGATCC 1 cut(s) 520
BanII GRGCYC 1 cut(s) 22
BbsI GAAGAC 1 cut(s) 41
BbvI GCAGC 1 cut(s) 82
BccI CCATC 4 cut(s) 239, 293, 389, 461
BceAI ACGGC 1 cut(s) 75
BfaI CTAG 1 cut(s) 374
BisI GCNGC 1 cut(s) 96
BlsI GCNGC 1 cut(s) 97
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 4 cut(s) 18, 19, 183, 302
BmiI GGNNCC 3 cut(s) 20, 21, 522
BmrI ACTGGG 1 cut(s) 518
BmsI GCATC 1 cut(s) 503
BmuI ACTGGG 1 cut(s) 518
BpiI GAAGAC 1 cut(s) 41
BpmI CTGGAG 1 cut(s) 84
Bpu10I CCTNAGC 1 cut(s) 74
BsaJI CCNNGG 2 cut(s) 179, 330
BsaWI WCCGGW 1 cut(s) 421
Bsc4I CCNNNNNNNGG 1 cut(s) 62
Bse1I ACTGG 1 cut(s) 513
BseAI TCCGGA 1 cut(s) 421
BseDI CCNNGG 2 cut(s) 179, 330
BseGI GGATG 3 cut(s) 231, 304, 518
BseLI CCNNNNNNNGG 1 cut(s) 62
BseMII CTCAG 2 cut(s) 377, 517
BseNI ACTGG 1 cut(s) 513
BseSI GKGCMC 2 cut(s) 22, 575
BseXI GCAGC 1 cut(s) 82
BseYI CCCAGC 1 cut(s) 524
BshFI GGCC 2 cut(s) 20, 185
BsiSI CCGG 1 cut(s) 422
BslFI GGGAC 1 cut(s) 384
BslI CCNNNNNNNGG 1 cut(s) 62
BsmFI GGGAC 1 cut(s) 384
BsmI GAATGC 2 cut(s) 243, 553
BsnI GGCC 2 cut(s) 20, 185
Bsp120I GGGCCC 1 cut(s) 18
Bsp1286I GDGCHC 2 cut(s) 22, 575
Bsp13I TCCGGA 1 cut(s) 421
Bsp143I GATC 4 cut(s) 484, 499, 520, 580
BspANI GGCC 2 cut(s) 20, 185
BspCNI CTCAG 2 cut(s) 378, 516
BspEI TCCGGA 1 cut(s) 421
BspLI GGNNCC 3 cut(s) 20, 21, 522
BspPI GGATC 3 cut(s) 515, 528, 588
BspQI GCTCTTC 1 cut(s) 375
BsrI ACTGG 1 cut(s) 513
BssECI CCNNGG 2 cut(s) 179, 330
BssMI GATC 4 cut(s) 484, 499, 520, 580
BssT1I CCWWGG 2 cut(s) 179, 330
Bst4CI ACNGT 2 cut(s) 47, 472
Bst6I CTCTTC 1 cut(s) 375
BstC8I GCNNGC 2 cut(s) 255, 403
BstDEI CTNAG 5 cut(s) 74, 191, 386, 503, 591
BstF5I GGATG 3 cut(s) 231, 304, 518
BstKTI GATC 4 cut(s) 487, 502, 523, 583
BstMBI GATC 4 cut(s) 484, 499, 520, 580
BstNSI RCATGY 1 cut(s) 257
BstSLI GKGCMC 2 cut(s) 22, 575
BstV1I GCAGC 1 cut(s) 82
BstV2I GAAGAC 1 cut(s) 41
BstX2I RGATCY 2 cut(s) 520, 580
BstYI RGATCY 2 cut(s) 520, 580
BsuRI GGCC 2 cut(s) 20, 185
BtsCI GGATG 3 cut(s) 231, 304, 518
Cac8I GCNNGC 2 cut(s) 255, 403
Cfr13I GGNCC 4 cut(s) 18, 19, 183, 302
Csp6I GTAC 1 cut(s) 450
CviAII CATG 4 cut(s) 40, 187, 254, 457
CviQI GTAC 1 cut(s) 450
DdeI CTNAG 5 cut(s) 74, 191, 386, 503, 591
DpnI GATC 4 cut(s) 486, 501, 522, 582
DpnII GATC 4 cut(s) 484, 499, 520, 580
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
Eco130I CCWWGG 2 cut(s) 179, 330
Eco24I GRGCYC 1 cut(s) 22
Eco47I GGWCC 1 cut(s) 302
EcoO109I RGGNCCY 2 cut(s) 18, 19
EcoRI GAATTC 1 cut(s) 356
EcoT14I CCWWGG 2 cut(s) 179, 330
EcoT38I GRGCYC 1 cut(s) 22
ErhI CCWWGG 2 cut(s) 179, 330
FaeI CATG 4 cut(s) 43, 190, 257, 460
FaiI YATR 8 cut(s) 28, 41, 161, 188, 226, 255, 458, 540
FaqI GGGAC 1 cut(s) 384
FatI CATG 4 cut(s) 39, 186, 253, 456
FblI GTMKAC 1 cut(s) 558
Fnu4HI GCNGC 1 cut(s) 96
FokI GGATG 3 cut(s) 218, 311, 525
FriOI GRGCYC 1 cut(s) 22
Fsp4HI GCNGC 1 cut(s) 96
FspBI CTAG 1 cut(s) 374
GluI GCNGC 1 cut(s) 96
GsaI CCCAGC 1 cut(s) 528
GsuI CTGGAG 1 cut(s) 84
HaeIII GGCC 2 cut(s) 20, 185
HapII CCGG 1 cut(s) 422
Hin1II CATG 4 cut(s) 43, 190, 257, 460
HinfI GANTC 4 cut(s) 103, 215, 425, 587
HpaII CCGG 1 cut(s) 422
HphI GGTGA 3 cut(s) 185, 494, 522
Hpy166II GTNNAC 1 cut(s) 559
Hpy188I TCNGA 3 cut(s) 249, 261, 506
Hpy188III TCNNGA 2 cut(s) 422, 584
Hpy8I GTNNAC 1 cut(s) 559
Hpy99I CGWCG 1 cut(s) 566
HpyAV CCTTC 3 cut(s) 9, 145, 470
HpyCH4III ACNGT 2 cut(s) 47, 472
HpyCH4IV ACGT 1 cut(s) 569
HpyCH4V TGCA 4 cut(s) 98, 243, 492, 516
HpyF3I CTNAG 5 cut(s) 74, 191, 386, 503, 591
HpySE526I ACGT 1 cut(s) 569
Hsp92II CATG 4 cut(s) 43, 190, 257, 460
Kpn2I TCCGGA 1 cut(s) 421
Kzo9I GATC 4 cut(s) 484, 499, 520, 580
LguI GCTCTTC 1 cut(s) 375
LpnPI CCDG 7 cut(s) 36, 48, 105, 435, 494, 538, 569
Lsp1109I GCAGC 1 cut(s) 82
LweI GCATC 1 cut(s) 503
MaeI CTAG 1 cut(s) 374
MaeII ACGT 1 cut(s) 569
MalI GATC 4 cut(s) 486, 501, 522, 582
MboI GATC 4 cut(s) 484, 499, 520, 580
MboII GAAGA 4 cut(s) 41, 131, 352, 392
MfeI CAATTG 1 cut(s) 351
MflI RGATCY 2 cut(s) 520, 580
MhlI GDGCHC 2 cut(s) 22, 575
MluCI AATT 6 cut(s) 237, 267, 317, 351, 356, 463
MnlI CCTC 6 cut(s) 63, 137, 197, 361, 473, 543
MroI TCCGGA 1 cut(s) 421
MseI TTAA 2 cut(s) 150, 266
MslI CAYNNNNRTG 1 cut(s) 437
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 1 cut(s) 422
MunI CAATTG 1 cut(s) 351
Mva1269I GAATGC 2 cut(s) 243, 553
NdeII GATC 4 cut(s) 484, 499, 520, 580
NlaIII CATG 4 cut(s) 43, 190, 257, 460
NlaIV GGNNCC 3 cut(s) 20, 21, 522
NspI RCATGY 1 cut(s) 257
PaeI GCATGC 1 cut(s) 257
PciSI GCTCTTC 1 cut(s) 375
PctI GAATGC 2 cut(s) 243, 553
PfeI GAWTC 4 cut(s) 103, 215, 425, 587
PkrI GCNGC 1 cut(s) 97
PspFI CCCAGC 1 cut(s) 524
PspN4I GGNNCC 3 cut(s) 20, 21, 522
PspOMI GGGCCC 1 cut(s) 18
PspPI GGNCC 4 cut(s) 18, 19, 183, 302
PsuI RGATCY 2 cut(s) 520, 580
PvuII CAGCTG 1 cut(s) 95
RsaI GTAC 1 cut(s) 451
RsaNI GTAC 1 cut(s) 450
RseI CAYNNNNRTG 1 cut(s) 437
SapI GCTCTTC 1 cut(s) 375
SaqAI TTAA 2 cut(s) 150, 266
SatI GCNGC 1 cut(s) 96
Sau3AI GATC 4 cut(s) 484, 499, 520, 580
Sau96I GGNCC 4 cut(s) 18, 19, 183, 302
SduI GDGCHC 2 cut(s) 22, 575
SfaNI GCATC 1 cut(s) 503
SinI GGWCC 1 cut(s) 302
SmiMI CAYNNNNRTG 1 cut(s) 437
SphI GCATGC 1 cut(s) 257
Sse9I AATT 6 cut(s) 237, 267, 317, 351, 356, 463
SspMI CTAG 1 cut(s) 374
StyI CCWWGG 2 cut(s) 179, 330
TaaI ACNGT 2 cut(s) 47, 472
TaiI ACGT 1 cut(s) 572
TaqI TCGA 2 cut(s) 202, 428
TasI AATT 6 cut(s) 237, 267, 317, 351, 356, 463
TfiI GAWTC 4 cut(s) 103, 215, 425, 587
Tru1I TTAA 2 cut(s) 150, 266
Tru9I TTAA 2 cut(s) 150, 266
TseI GCWGC 1 cut(s) 95
TspDTI ATGAA 4 cut(s) 56, 132, 264, 306
TspGWI ACGGA 2 cut(s) 533, 579
VpaK11BI GGWCC 1 cut(s) 302
XapI RAATTY 3 cut(s) 237, 356, 463
XceI RCATGY 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 558
XspI CTAG 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.