MD17G1228200.v1.1

Trimethylguanosine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007787
Physical Location & Seq
Reverse (-)
27580426 .. 27583854
3429 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1228200.v1.1.491

Sequence Viewer

Length: 807 bp
ATGCAGAAGAAGCTTTCAAGGCGGCGAAGAGCCACTCGTCGTCCTAAGAAATGCAACAAGCTCTTACACCACCCACGTCAGATTTATAGGAGAAGGAAGTTACTTGAACAAGGGTTGAGTCCCCTTGTTGAGAAGTATTGGTTTCAGAGATATGATCTCTTCTCGAGATACGACGAGGGGATCAGAATGGATGAGCAGGGCTGGTACTCTGTCACGCCTGAACAGATTGCAATCGGACACGCCAAGAGGTGCCAAAGAACAACTGTTATCGATTGCTTCGCGGGCGTTGGCGGCAATGCCATTCAGTTTGCTTCACTGGTCTGGTTTTGGATGTTGAATTCTGTCGGGGTTTCATCTTTGATGGACAAGAGTAGGCATTGTCACGTTGTTGCTATTGAAATTGATCCCCTAAAGGTGGATATGGCCATTAACAATGCAAAAATATATGGAGTGGAAGATTACATTGATTTCATTGTTGGAGACTTTTTTGAACTTGCACCATCGTTGAAGGGGGATGTAGTGTTCCTTTCACCACCTTGGGGAGGCCCATCGTACACAAGGGTAAAGAAATTTACACTTGACTTACTCAAGCCAAAAGATGGGCACGCAATATTTCAAGCTGCTCAAGCGATAACGCCCAACATCATTATGTACTTACCGCGGAATGTAGACTTACACCAAGTGGAAGAACTCTGCTGGTTGTCTTCTCCTCCTCTAGACGTTGAGATTGAAGAAAATTATGTGCAGAGCTCTCTAAAGGCTATAACAGTCTACTTCAGTGGTGCATCATCCACTCAATGTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Methyltransf_15

Protein Analysis

269

Amino Acids

30.8

Weight (kDa)

9.05

Isoelectric Point (pI)

50.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_15 PF09445 87 - 260 3.4e-34 RNA cap guanine-N2 methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 249
AccB7I CCANNNNNTGG 1 cut(s) 599
AccI GTMKAC 2 cut(s) 669, 771
AccII CGCG 2 cut(s) 281, 661
AciI CCGC 5 cut(s) 22, 281, 291, 659, 661
AclWI GGATC 2 cut(s) 188, 398
AcoI YGGCCR 1 cut(s) 423
AcsI RAATTY 2 cut(s) 337, 569
AcuI CTGAAG 1 cut(s) 760
AdeI CACNNNGTG 1 cut(s) 682
AfaI GTAC 3 cut(s) 206, 554, 653
AfiI CCNNNNNNNGG 4 cut(s) 415, 539, 542, 599
AgsI TTSAA 8 cut(s) 18, 107, 337, 398, 491, 508, 617, 731
AjiI CACGTC 1 cut(s) 77
AloI GAACNNNNNNTCC 2 cut(s) 506, 538
AluBI AGCT 4 cut(s) 13, 61, 620, 750
AluI AGCT 4 cut(s) 13, 61, 620, 750
Alw21I GWGCWC 1 cut(s) 752
Alw26I GTCTC 1 cut(s) 474
AlwI GGATC 2 cut(s) 188, 398
Ama87I CYCGRG 1 cut(s) 163
AoxI GGCC 2 cut(s) 423, 544
ApeKI GCWGC 1 cut(s) 620
ApoI RAATTY 2 cut(s) 337, 569
AspS9I GGNCC 1 cut(s) 545
AsuHPI GGTGA 1 cut(s) 522
AvaI CYCGRG 1 cut(s) 163
BaeGI GKGCMC 1 cut(s) 606
BalI TGGCCA 1 cut(s) 425
BanI GGYRCC 1 cut(s) 249
BanII GRGCYC 1 cut(s) 752
BbsI GAAGAC 1 cut(s) 696
Bbv12I GWGCWC 1 cut(s) 752
BbvI GCAGC 1 cut(s) 607
BccI CCATC 4 cut(s) 355, 508, 556, 593
BcoDI GTCTC 1 cut(s) 474
BfaI CTAG 1 cut(s) 716
BisI GCNGC 3 cut(s) 23, 292, 621
BlsI GCNGC 3 cut(s) 24, 293, 622
BmeT110I CYCGRG 1 cut(s) 163
BmgBI CACGTC 1 cut(s) 77
BmgT120I GGNCC 1 cut(s) 545
BmiI GGNNCC 1 cut(s) 251
BmsI GCATC 1 cut(s) 794
BpiI GAAGAC 1 cut(s) 696
BpuEI CTTGAG 2 cut(s) 572, 609
Bsa29I ATCGAT 1 cut(s) 270
BsaBI GATNNNNATC 1 cut(s) 230
BsaJI CCNNGG 2 cut(s) 536, 659
Bsc4I CCNNNNNNNGG 4 cut(s) 415, 539, 542, 599
Bse1I ACTGG 1 cut(s) 321
Bse3DI GCAATG 1 cut(s) 301
Bse8I GATNNNNATC 1 cut(s) 230
BseCI ATCGAT 1 cut(s) 270
BseDI CCNNGG 2 cut(s) 536, 659
BseGI GGATG 4 cut(s) 196, 336, 520, 788
BseJI GATNNNNATC 1 cut(s) 230
BseLI CCNNNNNNNGG 4 cut(s) 415, 539, 542, 599
BseMI GCAATG 1 cut(s) 301
BseNI ACTGG 1 cut(s) 321
BseRI GAGGAG 2 cut(s) 699, 702
BseSI GKGCMC 1 cut(s) 606
BseXI GCAGC 1 cut(s) 607
BsgI GTGCAG 1 cut(s) 764
Bsh1236I CGCG 2 cut(s) 281, 661
BshFI GGCC 2 cut(s) 425, 546
BshNI GGYRCC 1 cut(s) 249
BshVI ATCGAT 1 cut(s) 270
BsiHKAI GWGCWC 1 cut(s) 752
BsiHKCI CYCGRG 1 cut(s) 163
BslFI GGGAC 1 cut(s) 105
BslI CCNNNNNNNGG 4 cut(s) 415, 539, 542, 599
BsmAI GTCTC 1 cut(s) 474
BsmFI GGGAC 1 cut(s) 105
BsnI GGCC 2 cut(s) 425, 546
BsoBI CYCGRG 1 cut(s) 163
Bsp1286I GDGCHC 2 cut(s) 606, 752
Bsp143I GATC 3 cut(s) 154, 180, 403
BspACI CCGC 5 cut(s) 22, 281, 291, 659, 661
BspANI GGCC 2 cut(s) 425, 546
BspDI ATCGAT 1 cut(s) 270
BspFNI CGCG 2 cut(s) 281, 661
BspLI GGNNCC 1 cut(s) 251
BspPI GGATC 2 cut(s) 188, 398
BspQI GCTCTTC 1 cut(s) 22
BspT107I GGYRCC 1 cut(s) 249
BsrDI GCAATG 1 cut(s) 301
BsrI ACTGG 1 cut(s) 321
BssECI CCNNGG 2 cut(s) 536, 659
BssMI GATC 3 cut(s) 154, 180, 403
BssT1I CCWWGG 1 cut(s) 536
Bst4CI ACNGT 2 cut(s) 265, 769
Bst6I CTCTTC 2 cut(s) 22, 164
BstC8I GCNNGC 2 cut(s) 283, 606
BstDEI CTNAG 1 cut(s) 45
BstDSI CCRYGG 1 cut(s) 659
BstENI CCTNNNNNAGG 1 cut(s) 540
BstF5I GGATG 4 cut(s) 196, 336, 520, 788
BstFNI CGCG 2 cut(s) 281, 661
BstKTI GATC 3 cut(s) 157, 183, 406
BstMAI GTCTC 1 cut(s) 474
BstMBI GATC 3 cut(s) 154, 180, 403
BstMWI GCNNNNNNNGC 5 cut(s) 10, 19, 282, 291, 626
BstSLI GKGCMC 1 cut(s) 606
BstUI CGCG 2 cut(s) 281, 661
BstV1I GCAGC 1 cut(s) 607
BstV2I GAAGAC 1 cut(s) 696
Bsu15I ATCGAT 1 cut(s) 270
BsuRI GGCC 2 cut(s) 425, 546
BsuTUI ATCGAT 1 cut(s) 270
BtgI CCRYGG 1 cut(s) 659
BtrI CACGTC 1 cut(s) 77
BtsCI GGATG 4 cut(s) 196, 336, 520, 788
BtsIMutI CAGTG 2 cut(s) 314, 784
Cac8I GCNNGC 2 cut(s) 283, 606
Cfr13I GGNCC 1 cut(s) 545
Cfr42I CCGCGG 1 cut(s) 662
ClaI ATCGAT 1 cut(s) 270
Csp6I GTAC 3 cut(s) 205, 553, 652
CviQI GTAC 3 cut(s) 205, 553, 652
DdeI CTNAG 1 cut(s) 45
DpnI GATC 3 cut(s) 156, 182, 405
DpnII GATC 3 cut(s) 154, 180, 403
DraIII CACNNNGTG 1 cut(s) 682
EaeI YGGCCR 1 cut(s) 423
Eam1104I CTCTTC 2 cut(s) 22, 164
EarI CTCTTC 2 cut(s) 22, 164
Ecl136II GAGCTC 1 cut(s) 750
Eco130I CCWWGG 1 cut(s) 536
Eco24I GRGCYC 1 cut(s) 752
Eco53kI GAGCTC 1 cut(s) 750
Eco57I CTGAAG 1 cut(s) 760
Eco88I CYCGRG 1 cut(s) 163
EcoICRI GAGCTC 1 cut(s) 750
EcoNI CCTNNNNNAGG 1 cut(s) 540
EcoRI GAATTC 1 cut(s) 337
EcoT14I CCWWGG 1 cut(s) 536
EcoT38I GRGCYC 1 cut(s) 752
ErhI CCWWGG 1 cut(s) 536
FaqI GGGAC 1 cut(s) 105
FauI CCCGC 1 cut(s) 274
FblI GTMKAC 2 cut(s) 669, 771
Fnu4HI GCNGC 3 cut(s) 23, 292, 621
FokI GGATG 4 cut(s) 203, 343, 527, 775
FriOI GRGCYC 1 cut(s) 752
Fsp4HI GCNGC 3 cut(s) 23, 292, 621
FspBI CTAG 1 cut(s) 716
GluI GCNGC 3 cut(s) 23, 292, 621
HaeIII GGCC 2 cut(s) 425, 546
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 1 cut(s) 118
HphI GGTGA 1 cut(s) 522
Hpy166II GTNNAC 3 cut(s) 555, 670, 772
Hpy188I TCNGA 4 cut(s) 81, 147, 185, 236
Hpy188III TCNNGA 3 cut(s) 163, 165, 716
Hpy8I GTNNAC 3 cut(s) 555, 670, 772
Hpy99I CGWCG 2 cut(s) 42, 176
HpyAV CCTTC 2 cut(s) 87, 502
HpyCH4III ACNGT 2 cut(s) 265, 769
HpyCH4IV ACGT 3 cut(s) 76, 384, 720
HpyCH4V TGCA 7 cut(s) 4, 54, 230, 437, 497, 745, 785
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 19, 282, 291, 626
HpyF3I CTNAG 1 cut(s) 45
HpySE526I ACGT 3 cut(s) 76, 384, 720
KspI CCGCGG 1 cut(s) 662
Kzo9I GATC 3 cut(s) 154, 180, 403
LguI GCTCTTC 1 cut(s) 22
LpnPI CCDG 6 cut(s) 182, 187, 231, 302, 307, 682
Lsp1109I GCAGC 1 cut(s) 607
LweI GCATC 1 cut(s) 794
MaeI CTAG 1 cut(s) 716
MaeII ACGT 3 cut(s) 76, 384, 720
MaeIII GTNAC 3 cut(s) 99, 211, 380
MalI GATC 3 cut(s) 156, 182, 405
MboI GATC 3 cut(s) 154, 180, 403
MboII GAAGA 7 cut(s) 19, 39, 151, 467, 696, 698, 743
MhlI GDGCHC 2 cut(s) 606, 752
MlsI TGGCCA 1 cut(s) 425
MluCI AATT 4 cut(s) 337, 399, 569, 736
MluNI TGGCCA 1 cut(s) 425
MlyI GAGTC 1 cut(s) 127
MmeI TCCRAC 1 cut(s) 457
MnlI CCTC 5 cut(s) 169, 240, 536, 720, 723
Mox20I TGGCCA 1 cut(s) 425
MscI TGGCCA 1 cut(s) 425
MseI TTAA 1 cut(s) 429
MslI CAYNNNNRTG 1 cut(s) 647
Msp20I TGGCCA 1 cut(s) 425
MspA1I CMGCKG 1 cut(s) 661
MvnI CGCG 2 cut(s) 281, 661
MwoI GCNNNNNNNGC 5 cut(s) 10, 19, 282, 291, 626
NdeII GATC 3 cut(s) 154, 180, 403
NlaIV GGNNCC 1 cut(s) 251
NmuCI GTSAC 2 cut(s) 211, 380
PaeR7I CTCGAG 1 cut(s) 163
PciSI GCTCTTC 1 cut(s) 22
PflMI CCANNNNNTGG 1 cut(s) 599
PkrI GCNGC 3 cut(s) 24, 293, 622
PleI GAGTC 1 cut(s) 126
PpsI GAGTC 1 cut(s) 126
Psp124BI GAGCTC 1 cut(s) 752
PspN4I GGNNCC 1 cut(s) 251
PspPI GGNCC 1 cut(s) 545
RsaI GTAC 3 cut(s) 206, 554, 653
RsaNI GTAC 3 cut(s) 205, 553, 652
RseI CAYNNNNRTG 1 cut(s) 647
SacI GAGCTC 1 cut(s) 752
SacII CCGCGG 1 cut(s) 662
SapI GCTCTTC 1 cut(s) 22
SaqAI TTAA 1 cut(s) 429
SatI GCNGC 3 cut(s) 23, 292, 621
Sau3AI GATC 3 cut(s) 154, 180, 403
Sau96I GGNCC 1 cut(s) 545
SchI GAGTC 1 cut(s) 127
SduI GDGCHC 2 cut(s) 606, 752
SfaNI GCATC 1 cut(s) 794
Sfr274I CTCGAG 1 cut(s) 163
Sfr303I CCGCGG 1 cut(s) 662
SgrBI CCGCGG 1 cut(s) 662
SlaI CTCGAG 1 cut(s) 163
SmiMI CAYNNNNRTG 1 cut(s) 647
SmlI CTYRAG 3 cut(s) 163, 587, 624
SmoI CTYRAG 3 cut(s) 163, 587, 624
Sse9I AATT 4 cut(s) 337, 399, 569, 736
SsiI CCGC 5 cut(s) 22, 281, 291, 659, 661
SspI AATATT 1 cut(s) 612
SspMI CTAG 1 cut(s) 716
SstI GAGCTC 1 cut(s) 752
StyI CCWWGG 1 cut(s) 536
TaaI ACNGT 2 cut(s) 265, 769
TaiI ACGT 3 cut(s) 79, 387, 723
TaqI TCGA 2 cut(s) 164, 270
TasI AATT 4 cut(s) 337, 399, 569, 736
TatI WGTACW 1 cut(s) 651
TauI GCSGC 2 cut(s) 25, 294
Tru1I TTAA 1 cut(s) 429
Tru9I TTAA 1 cut(s) 429
TscAI CASTG 2 cut(s) 321, 784
TseFI GTSAC 2 cut(s) 211, 380
TseI GCWGC 1 cut(s) 620
Tsp45I GTSAC 2 cut(s) 211, 380
TspDTI ATGAA 2 cut(s) 342, 460
TspRI CASTG 2 cut(s) 321, 784
Van91I CCANNNNNTGG 1 cut(s) 599
XagI CCTNNNNNAGG 1 cut(s) 540
XapI RAATTY 2 cut(s) 337, 569
XbaI TCTAGA 1 cut(s) 715
XhoI CTCGAG 1 cut(s) 163
XmiI GTMKAC 2 cut(s) 669, 771
XspI CTAG 1 cut(s) 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.