Rorug02G0301900

Trimethylguanosine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007787
Physical Location & Seq
Reverse (-)
33708054 .. 33708808
755 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0301900.1

Sequence Viewer

Length: 300 bp
ATGGGTTTCCGGCTGCCTGGAATTGTTAGCCCCAAGAGAAGTCTTACCCGATCTTCATCGAATGCGAACAAGACAGCATCAAAGACCTTAGATATCCCAAAAGGCTACTTTGCGGTCTATGTTGGAGAGAGCCAGAAGAAGCGATTTGTGATTCCAATATCTTACTTGAACCAACCTTCATTCATGGATTTGCTGAGTCAAGCTGAAGAAGAATTTGGATACAATCATCCCATGGGCGGTATCACAATCCCTTGCAGTGAGGACACTTTCCTTGATCTCACTTCCAGCTTAAGTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Methyltransf_15

Protein Analysis

99

Amino Acids

10.91

Weight (kDa)

7.86

Isoelectric Point (pI)

50.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 15 - 97 3e-24 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 113, 237
AcsI RAATTY 1 cut(s) 212
AcuI CTGAAG 1 cut(s) 225
AfiI CCNNNNNNNGG 1 cut(s) 236
AflII CTTAAG 1 cut(s) 289
AgsI TTSAA 1 cut(s) 169
AjnI CCWGG 1 cut(s) 16
AjuI GAANNNNNNNTTGG 2 cut(s) 198, 230
AluBI AGCT 2 cut(s) 203, 288
AluI AGCT 2 cut(s) 203, 288
ApeKI GCWGC 1 cut(s) 13
ApoI RAATTY 1 cut(s) 212
BciT130I CCWGG 1 cut(s) 18
BciVI GTATCC 1 cut(s) 212
BfrI CTTAAG 1 cut(s) 289
BfuI GTATCC 1 cut(s) 212
BisI GCNGC 1 cut(s) 14
BlsI GCNGC 1 cut(s) 15
Bme1390I CCNGG 1 cut(s) 18
BmrFI CCNGG 1 cut(s) 18
BmsI GCATC 1 cut(s) 86
BsaBI GATNNNNATC 1 cut(s) 55
BsaJI CCNNGG 1 cut(s) 231
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse8I GATNNNNATC 1 cut(s) 55
BseBI CCWGG 1 cut(s) 18
BseDI CCNNGG 1 cut(s) 231
BseGI GGATG 1 cut(s) 226
BseJI GATNNNNATC 1 cut(s) 55
BseLI CCNNNNNNNGG 1 cut(s) 236
BseMII CTCAG 1 cut(s) 185
BsiSI CCGG 1 cut(s) 10
BslI CCNNNNNNNGG 1 cut(s) 236
BsmI GAATGC 1 cut(s) 67
Bsp143I GATC 2 cut(s) 50, 274
Bsp19I CCATGG 1 cut(s) 231
BspACI CCGC 2 cut(s) 113, 237
BspCNI CTCAG 1 cut(s) 186
BspTI CTTAAG 1 cut(s) 289
BssECI CCNNGG 1 cut(s) 231
BssMI GATC 2 cut(s) 50, 274
BssT1I CCWWGG 1 cut(s) 231
Bst2UI CCWGG 1 cut(s) 18
BstAFI CTTAAG 1 cut(s) 289
BstDEI CTNAG 2 cut(s) 88, 194
BstDSI CCRYGG 1 cut(s) 231
BstF5I GGATG 1 cut(s) 226
BstKTI GATC 2 cut(s) 53, 277
BstMBI GATC 2 cut(s) 50, 274
BstNI CCWGG 1 cut(s) 18
BstSCI CCNGG 1 cut(s) 16
BsuI GTATCC 1 cut(s) 212
BtgI CCRYGG 1 cut(s) 231
BtsCI GGATG 1 cut(s) 226
BtsI GCAGTG 1 cut(s) 262
BtsIMutI CAGTG 1 cut(s) 262
CviAII CATG 2 cut(s) 184, 232
CviJI RGCY 6 cut(s) 13, 30, 105, 132, 203, 288
CviKI_1 RGCY 6 cut(s) 13, 30, 105, 132, 203, 288
DdeI CTNAG 2 cut(s) 88, 194
DpnI GATC 2 cut(s) 52, 276
DpnII GATC 2 cut(s) 50, 274
Eco130I CCWWGG 1 cut(s) 231
Eco32I GATATC 1 cut(s) 94
Eco57I CTGAAG 1 cut(s) 225
EcoRII CCWGG 1 cut(s) 16
EcoRV GATATC 1 cut(s) 94
EcoT14I CCWWGG 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 231
FaeI CATG 2 cut(s) 187, 235
FaiI YATR 3 cut(s) 120, 185, 233
FatI CATG 2 cut(s) 183, 231
Fnu4HI GCNGC 1 cut(s) 14
FokI GGATG 1 cut(s) 213
Fsp4HI GCNGC 1 cut(s) 14
GluI GCNGC 1 cut(s) 14
HapII CCGG 1 cut(s) 10
Hin1II CATG 2 cut(s) 187, 235
HinfI GANTC 2 cut(s) 151, 196
HpaII CCGG 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 186
HpyCH4V TGCA 1 cut(s) 255
HpyF3I CTNAG 2 cut(s) 88, 194
Hsp92II CATG 2 cut(s) 187, 235
Kzo9I GATC 2 cut(s) 50, 274
LpnPI CCDG 4 cut(s) 3, 23, 30, 146
LweI GCATC 1 cut(s) 86
MalI GATC 2 cut(s) 52, 276
MboI GATC 2 cut(s) 50, 274
MboII GAAGA 4 cut(s) 45, 148, 218, 221
MluCI AATT 2 cut(s) 21, 212
MlyI GAGTC 1 cut(s) 205
MmeI TCCRAC 1 cut(s) 103
MnlI CCTC 1 cut(s) 253
MseI TTAA 1 cut(s) 290
MspCI CTTAAG 1 cut(s) 289
MspI CCGG 1 cut(s) 10
MspR9I CCNGG 1 cut(s) 18
Mva1269I GAATGC 1 cut(s) 67
MvaI CCWGG 1 cut(s) 18
NcoI CCATGG 1 cut(s) 231
NdeII GATC 2 cut(s) 50, 274
NlaIII CATG 2 cut(s) 187, 235
PctI GAATGC 1 cut(s) 67
PfeI GAWTC 1 cut(s) 151
PkrI GCNGC 1 cut(s) 15
PleI GAGTC 1 cut(s) 204
PpsI GAGTC 1 cut(s) 204
Psp6I CCWGG 1 cut(s) 16
PspGI CCWGG 1 cut(s) 16
SaqAI TTAA 1 cut(s) 290
SatI GCNGC 1 cut(s) 14
Sau3AI GATC 2 cut(s) 50, 274
SchI GAGTC 1 cut(s) 205
ScrFI CCNGG 1 cut(s) 18
SetI ASST 4 cut(s) 89, 178, 205, 290
SfaNI GCATC 1 cut(s) 86
SmlI CTYRAG 1 cut(s) 289
SmoI CTYRAG 1 cut(s) 289
Sse9I AATT 2 cut(s) 21, 212
SsiI CCGC 2 cut(s) 113, 237
StyD4I CCNGG 1 cut(s) 16
StyI CCWWGG 1 cut(s) 231
TaqI TCGA 1 cut(s) 59
TasI AATT 2 cut(s) 21, 212
TfiI GAWTC 1 cut(s) 151
Tru1I TTAA 1 cut(s) 290
Tru9I TTAA 1 cut(s) 290
TscAI CASTG 1 cut(s) 262
TseI GCWGC 1 cut(s) 13
TspDTI ATGAA 3 cut(s) 45, 168, 172
TspRI CASTG 1 cut(s) 262
Vha464I CTTAAG 1 cut(s) 289
XapI RAATTY 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.