Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0004348

Physical Location & Seq

Genomic Coordinates Reverse (-)

28929774 .. 28945592

Gene Structure 15819 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1240200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2493 bp

ATGCATATCCTCCGCCACATCAAATTAAACCGAACACCACCGGATCTTTCTCTCGGGATCTGCGGGACCGAGCAATCTTGGCCGTCCAAATTGATCTTGCGGTTAGGAATAAACTTCGAGTTTTATTTAAACTTCGATTTTATTTTATTTTCCAGCGCTAAACTAAAAACACCAGACAGACAAGAGTCAGTCACAAGTCACAAGTCGCTATTCACAACTGCCGCAATAACTCCTCGAACCGCATTTCGGCTCATCACATCGCCGGAGAAGATGGAGAAGCCTCTGCTCAGCGGTGAAGACAGCGGCCTTCGGCGCCGCGGCGATCCATCCCCCGGCTACAAACGCCGCGCGGATGCCATCACCTCCGGCACTCCTTATCAGAAAGCTGCTGCACTCGTCGATTTGGCGGAAGATGGCATTGGGTTACCAGAGCAGATACTGGATCAATCAAACTTTCAGAGTGCCGCTAAGTACTACTTCCTTTACTTCCGATTCAGCATCCTTTGGGCCCTCCTCTATTTCGCCCTCATACTCCTCAACTTCCTCGAGAAACCTCTGTGGTGCTATAGGTATACCGACACTGTCTATACATGTAGTGACAGAGACTACTTCTATCTTGGCCAATTGCCGTACTTGAATTCTCTGGAGTCTCTTATTTATGAGGGAATAACACTCATTGTACTCGTAGTGCACATTTTCTTTCCAATTTCGTATGAAGGGTTCCTCTATTGGAAAAATCCTCTTACCATATTTAAGGTCACATGCCTACTGATTTTGGTTGCTGATATGCTGGTTTATGCTCTCTATGTGTCTTCGGTGGCCTTTGATTCCCTTCCTTTCAGGATTGCACCATACATCAGAGTTATCTTTTTCATATTGAATTTCAGGGAATTGCGGTCATGCATGCTCATCCTGGCTGGAATGCTTGGCACGTACTTGAACGTCTTGGCTCTAGGGCTTTTATTTCTTCTGTTTTCCAGTTGGTTGGCCTATGTCATGTTTGAAGATACCAAGCAGGGGAAAACAGTATTTACTTCGTATGGTATTACATTGTATAAGATGTTTGTTTTATTCACCACATCCAACAATCCAGATGTCTGGATACCTGCCTACAAGGCTTCACGTTGGTATTCCTTGTTTTTTGTTCTATACGTGCTGCTGGGAGTTTACTTTGTCACTAACTTGATTCTTGCTGTTGTGTACGATAGCTTCAAAAATCAGCTTGTAAAACAAGTTTCTGAGATGGACCAGACAAGGAAAGCTATACTAAAGAAAGCCTTCAATTTATTAGACAAGGATAATGTAGGGTATCTCAATAAGGATGAGTGTATTCAACTATTTGAGGAACTAAATAAGTACAGGACTTTGCCAAAAATGACGAGAGAGGAATTTCAGCTGATATTTGATGAGCTGGATGACAGTGGAGACTTCAAGATCAACTTAGAAGAATTCTTTGATCTTTGCAATGCCATTGCCCTAAAATTCCCGAAGGAGGATACTCCCTCTTTGCTTGAAAAGTTCCCTTCATTCTACCATTCATTGTTCTCCGAAAAGTTGAAGGCCTTCGTACGGAGTCCCATATTTGGATACATAATATCCTTCATACTCATACTCAACCTGGCTACTGTTATTGTAGAAACAACGCTTGATATAGAAAACAGTACTGCTCAAGAGGTATGGCAAGTGGCGGAGTTTGTTTTTGGGTGGGTATATGTACTGGAAATGGCTCTGAAAGTCTATTCTTTTGGATTTGAGAACTATTGGAGGGATGGCCAAAATCGCTTTGATTTTGTGGTGACATGGATAATAGTTATTGGGGAAACTATAACTTTTGTGGCTCCTGAAGGACTAACTTTTCTTTCAAATGGAGAATGGATTCGCTACCTTCTCATTGCAAGAATGTTAAGATTGATTAGGCTCCTGATGCATATCCAGCGCTGCCGGGCATTTGTAGCTACCTTCTTAGCCCTCATACCGAGTTTGATGCCATACCTTGGAACCATATTCTGTGTCTTGTGTATCTATTGTATACTTGGTGTACAGATATTCGGTGGAATGGTGAATGATGGAAATACCTCTTTGGAAGGATCTGAGCTTTATGAGAATGATTACTTACTTTTCAACTTCAATGACTACCCCAATGGGATGGTAACACTTTTCAATTTGCTAGTCATGGGAAACTGGCAAGTATGGATGGAGAGTTATCAGGAACTAACAGGAACTTGGTGGAGCATTATATATTTTGTCAGCTTTTATCTTATAACCGTCTTACTGCTATTGAATTTGGTCGTGGCTTTTGTCCTGGAGGCTTTCTTTGCTGAAATGGATCTTGAGTCATCAGAAAAAAGTGAAGAAAATGGGAAGGAAGAGGAAGGACTAAAAGATCGACGTCGACCTTTGGGCTCCAAAACTCGGAGCCAGAGAATTGATGTTCTTCTTCACCATATGCTGAGTTCAGAGCTGACTGAGACACAACCCTCCAATGAAGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0001508 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005245 GO:0005261 GO:0005262 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008324 GO:0009791 GO:0009845 GO:0009889 GO:0009987 GO:0010119 GO:0010565 GO:0010817 GO:0012505 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019216 GO:0019217 GO:0019222 GO:0019722 GO:0019932 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0031090 GO:0031323 GO:0031326 GO:0032350 GO:0032501 GO:0032502 GO:0034220 GO:0035556 GO:0042304 GO:0042391 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046885 GO:0046890 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0051899 GO:0055085 GO:0062012 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0080090 GO:0080140 GO:0080141 GO:0086010 GO:0090351 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EF-hand_7 Ion_trans

Protein Analysis

831

Amino Acids

96.13

Weight (kDa)

5.3

Isoelectric Point (pI)

40.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	222 - 407	2.8e-16	Ion transport protein
EF-hand_7	PF13499	423 - 487	9.5e-10	EF-hand domain pair
Ion_trans	PF00520	526 - 772	9.2e-44	Ion transport protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004348)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G03560
fragaria_vesca	FvH4_6g26210 FvH4_6g26210 FvH4_6g26210 FvH4_6g26210
malus_domestica	MD09G1247300.v1.1 MD17G1240200.v1.1
prunus_persica	Prupe.3G138600_v2.0.a1 Prupe.3G138600_v2.0.a1 Prupe.3G138600_v2.0.a1
pyrus_communis	pycom09g16460 pycom17g24350
rosa_chinensis	RchiOBHm_Chr2g0126531 RchiOBHm_Chr2g0126551
rosa_laevigata	RLG00000018910 RLG00000018911
rosa_multiflora	Rmu_sc0002369.1_g000031 Rmu_sc0002369.1_g000036 Rmu_sc0007463.1_g000007
rosa_roxburghii	Rroxscaffold_2G00117090 Rroxscaffold_2G00117120
rosa_rugosa	Rorug02G0263600
rosa_samantha	Rh2AG320600 Rh2AG320700 Rh2BG329000 Rh2BG329100 Rh2CG308800 Rh2CG308900 Rh2DG348300 Rh2DG348400
rosa_wichuraiana	Rw2G025970 Rw2G025980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2261
AatII	GACGTC	1 cut(s)	2392
Acc36I	ACCTGC	1 cut(s)	1114
AccB1I	GGYRCC	1 cut(s)	312
AccB7I	CCANNNNNTGG	1 cut(s)	1994
AccI	GTMKAC	3 cut(s)	572, 2029, 2392
AccII	CGCG	3 cut(s)	318, 348, 350
AclWI	GGATC	6 cut(s)	51, 65, 317, 450, 2095, 2334
AcoI	YGGCCR	3 cut(s)	80, 619, 1771
AcsI	RAATTY	6 cut(s)	637, 880, 1388, 1448, 1481, 2281
AcuI	CTGAAG	1 cut(s)	1863
AcyI	GRCGYC	2 cut(s)	313, 2389
AfeI	AGCGCT	2 cut(s)	157, 1937
AfiI	CCNNNNNNNGG	8 cut(s)	246, 332, 1017, 1492, 1569, 1583, 1994, 2412
AflIII	ACRYGT	1 cut(s)	590
AjnI	CCWGG	3 cut(s)	912, 1617, 2301
AjuI	GAANNNNNNNTTGG	2 cut(s)	402, 434
Alw21I	GWGCWC	1 cut(s)	693
Alw26I	GTCTC	4 cut(s)	597, 654, 1419, 2462
Alw44I	GTGCAC	1 cut(s)	689
AlwI	GGATC	6 cut(s)	51, 65, 317, 450, 2095, 2334
AlwNI	CAGNNNCTG	1 cut(s)	439
Ama87I	CYCGRG	2 cut(s)	53, 545
Aor51HI	AGCGCT	2 cut(s)	157, 1937
AoxI	GGCC	8 cut(s)	80, 304, 507, 619, 819, 987, 1560, 1771
ApaI	GGGCCC	1 cut(s)	511
ApaLI	GTGCAC	1 cut(s)	689
ApeKI	GCWGC	4 cut(s)	386, 389, 1156, 1938
ApoI	RAATTY	6 cut(s)	637, 880, 1388, 1448, 1481, 2281
Asp700I	GAANNNNTTC	3 cut(s)	1277, 1562, 1875
AspLEI	GCGC	4 cut(s)	158, 315, 350, 1938
AspS9I	GGNCC	4 cut(s)	66, 507, 508, 1246
AsuC2I	CCSGG	2 cut(s)	333, 1943
AsuHPI	GGTGA	6 cut(s)	305, 352, 1066, 1807, 2071, 2432
AvaI	CYCGRG	2 cut(s)	53, 545
AvaII	GGWCC	2 cut(s)	66, 1246
BaeGI	GKGCMC	2 cut(s)	511, 693
BalI	TGGCCA	2 cut(s)	621, 1773
BanI	GGYRCC	1 cut(s)	312
BanII	GRGCYC	2 cut(s)	511, 2405
BarI	GAAGNNNNNNTAC	2 cut(s)	1193, 1225
BbsI	GAAGAC	2 cut(s)	303, 804
Bbv12I	GWGCWC	1 cut(s)	693
BbvI	GCAGC	4 cut(s)	373, 376, 1143, 1925
BccI	CCATC	9 cut(s)	265, 334, 365, 407, 1237, 1763, 2060, 2140, 2188
BceAI	ACGGC	2 cut(s)	67, 613
BcgI	CGANNNNNNTGC	2 cut(s)	1966, 2000
BciT130I	CCWGG	3 cut(s)	914, 1619, 2303
BciVI	GTATCC	3 cut(s)	1095, 1489, 1580
BcnI	CCSGG	2 cut(s)	333, 1943
BcoDI	GTCTC	4 cut(s)	597, 654, 1419, 2462
BfaI	CTAG	2 cut(s)	953, 2168
BfmI	CTRYAG	1 cut(s)	565
BfoI	RGCGCY	3 cut(s)	159, 316, 1939
BfuAI	ACCTGC	1 cut(s)	1114
BfuI	GTATCC	3 cut(s)	1095, 1489, 1580
BglI	GCCNNNNNGGC	1 cut(s)	1115
BlpI	GCTNAGC	1 cut(s)	287
BmcAI	AGTACT	2 cut(s)	473, 1663
Bme1390I	CCNGG	5 cut(s)	333, 914, 1619, 1943, 2303
Bme18I	GGWCC	2 cut(s)	66, 1246
BmeT110I	CYCGRG	2 cut(s)	53, 545
BmgT120I	GGNCC	4 cut(s)	66, 507, 508, 1246
BmiI	GGNNCC	9 cut(s)	67, 314, 509, 722, 1839, 1919, 1999, 2404, 2417
BmrFI	CCNGG	5 cut(s)	333, 914, 1619, 1943, 2303
BmsI	GCATC	4 cut(s)	343, 507, 1914, 1974
BoxI	GACNNNNGTC	1 cut(s)	184
BpiI	GAAGAC	2 cut(s)	303, 804
BpmI	CTGGAG	2 cut(s)	665, 2324
Bpu1102I	GCTNAGC	1 cut(s)	287
BpuEI	CTTGAG	2 cut(s)	1653, 2351
BpuMI	CCSGG	2 cut(s)	333, 1943
BsaAI	YACGTR	2 cut(s)	933, 1153
BsaBI	GATNNNNATC	1 cut(s)	1928
BsaHI	GRCGYC	2 cut(s)	313, 2389
BsaJI	CCNNGG	3 cut(s)	316, 331, 1993
BsaWI	WCCGGW	1 cut(s)	40
Bsc4I	CCNNNNNNNGG	8 cut(s)	246, 332, 1017, 1492, 1569, 1583, 1994, 2412
Bse1I	ACTGG	4 cut(s)	444, 978, 1722, 2186
Bse3DI	GCAATG	3 cut(s)	1470, 1471, 1890
Bse8I	GATNNNNATC	1 cut(s)	1928
BseBI	CCWGG	3 cut(s)	914, 1619, 2303
BseDI	CCNNGG	3 cut(s)	316, 331, 1993
BseJI	GATNNNNATC	1 cut(s)	1928
BseLI	CCNNNNNNNGG	8 cut(s)	246, 332, 1017, 1492, 1569, 1583, 1994, 2412
BseMI	GCAATG	3 cut(s)	1470, 1471, 1890
BseMII	CTCAG	5 cut(s)	301, 1230, 2082, 2441, 2457
BseNI	ACTGG	4 cut(s)	444, 978, 1722, 2186
BseRI	GAGGAG	3 cut(s)	222, 503, 524
BseSI	GKGCMC	2 cut(s)	511, 693
BseXI	GCAGC	4 cut(s)	373, 376, 1143, 1925
BseYI	CCCAGC	1 cut(s)	1159
BsgI	GTGCAG	1 cut(s)	375
Bsh1236I	CGCG	3 cut(s)	318, 348, 350
BshFI	GGCC	8 cut(s)	82, 306, 509, 621, 821, 989, 1562, 1773
BshNI	GGYRCC	1 cut(s)	312
BsiHKAI	GWGCWC	1 cut(s)	693
BsiHKCI	CYCGRG	2 cut(s)	53, 545
BsiSI	CCGG	5 cut(s)	41, 263, 333, 366, 1942
BsiWI	CGTACG	1 cut(s)	1567
BslFI	GGGAC	2 cut(s)	79, 1560
BslI	CCNNNNNNNGG	8 cut(s)	246, 332, 1017, 1492, 1569, 1583, 1994, 2412
BsmAI	GTCTC	4 cut(s)	597, 654, 1419, 2462
BsmFI	GGGAC	2 cut(s)	79, 1560
BsmI	GAATGC	1 cut(s)	927
BsnI	GGCC	8 cut(s)	82, 306, 509, 621, 821, 989, 1562, 1773
BsoBI	CYCGRG	2 cut(s)	53, 545
Bsp120I	GGGCCC	1 cut(s)	507
Bsp1286I	GDGCHC	3 cut(s)	511, 693, 2405
Bsp1407I	TGTACA	1 cut(s)	2038
Bsp1720I	GCTNAGC	1 cut(s)	287
BspANI	GGCC	8 cut(s)	82, 306, 509, 621, 821, 989, 1562, 1773
BspCNI	CTCAG	5 cut(s)	300, 1231, 2083, 2442, 2458
BspFNI	CGCG	3 cut(s)	318, 348, 350
BspLI	GGNNCC	9 cut(s)	67, 314, 509, 722, 1839, 1919, 1999, 2404, 2417
BspMI	ACCTGC	1 cut(s)	1114
BspPI	GGATC	6 cut(s)	51, 65, 317, 450, 2095, 2334
BspT107I	GGYRCC	1 cut(s)	312
BsrDI	GCAATG	3 cut(s)	1470, 1471, 1890
BsrGI	TGTACA	1 cut(s)	2038
BsrI	ACTGG	4 cut(s)	444, 978, 1722, 2186
BssECI	CCNNGG	3 cut(s)	316, 331, 1993
BssNAI	GTATAC	2 cut(s)	573, 2030
BssNI	GRCGYC	2 cut(s)	313, 2389
BssT1I	CCWWGG	1 cut(s)	1993
Bst1107I	GTATAC	2 cut(s)	573, 2030
Bst2UI	CCWGG	3 cut(s)	914, 1619, 2303
Bst4CI	ACNGT	6 cut(s)	583, 1027, 1421, 1627, 1661, 2266
Bst6I	CTCTTC	1 cut(s)	2361
BstACI	GRCGYC	2 cut(s)	313, 2389
BstAUI	TGTACA	1 cut(s)	2038
BstBAI	YACGTR	2 cut(s)	933, 1153
BstC8I	GCNNGC	1 cut(s)	905
BstDEI	CTNAG	8 cut(s)	287, 468, 1239, 1441, 1963, 2091, 2450, 2466
BstDSI	CCRYGG	1 cut(s)	316
BstEII	GGTNACC	1 cut(s)	423
BstFNI	CGCG	3 cut(s)	318, 348, 350
BstH2I	RGCGCY	3 cut(s)	159, 316, 1939
BstHHI	GCGC	4 cut(s)	158, 315, 350, 1938
BstMAI	GTCTC	4 cut(s)	597, 654, 1419, 2462
BstMWI	GCNNNNNNNGC	9 cut(s)	79, 312, 342, 1115, 1779, 1924, 1933, 1952, 2315
BstNI	CCWGG	3 cut(s)	914, 1619, 2303
BstNSI	RCATGY	3 cut(s)	594, 765, 907
BstPAI	GACNNNNGTC	1 cut(s)	184
BstPI	GGTNACC	1 cut(s)	423
BstSCI	CCNGG	5 cut(s)	331, 912, 1617, 1941, 2301
BstSFI	CTRYAG	1 cut(s)	565
BstSLI	GKGCMC	2 cut(s)	511, 693
BstUI	CGCG	3 cut(s)	318, 348, 350
BstV1I	GCAGC	4 cut(s)	373, 376, 1143, 1925
BstV2I	GAAGAC	2 cut(s)	303, 804
BstX2I	RGATCY	4 cut(s)	43, 57, 2087, 2326
BstXI	CCANNNNNNTGG	3 cut(s)	985, 1098, 2146
BstYI	RGATCY	4 cut(s)	43, 57, 2087, 2326
BstZ17I	GTATAC	2 cut(s)	573, 2030
BsuI	GTATCC	3 cut(s)	1095, 1489, 1580
BsuRI	GGCC	8 cut(s)	82, 306, 509, 621, 821, 989, 1562, 1773
BtgI	CCRYGG	1 cut(s)	316
BtgZI	GCGATG	1 cut(s)	243
BtsIMutI	CAGTG	2 cut(s)	579, 1426
BveI	ACCTGC	1 cut(s)	1114
Cac8I	GCNNGC	1 cut(s)	905
CaiI	CAGNNNCTG	1 cut(s)	439
CfoI	GCGC	4 cut(s)	158, 315, 350, 1938
Cfr13I	GGNCC	4 cut(s)	66, 507, 508, 1246
Cfr42I	CCGCGG	1 cut(s)	319
CviAII	CATG	7 cut(s)	591, 762, 900, 904, 997, 1800, 2173
DdeI	CTNAG	8 cut(s)	287, 468, 1239, 1441, 1963, 2091, 2450, 2466
DinI	GGCGCC	1 cut(s)	314
DraI	TTTAAA	1 cut(s)	129
EaeI	YGGCCR	3 cut(s)	80, 619, 1771
Eam1104I	CTCTTC	1 cut(s)	2361
EarI	CTCTTC	1 cut(s)	2361
EciI	GGCGGA	2 cut(s)	422, 1703
Eco130I	CCWWGG	1 cut(s)	1993
Eco147I	AGGCCT	1 cut(s)	1562
Eco24I	GRGCYC	2 cut(s)	511, 2405
Eco47I	GGWCC	2 cut(s)	66, 1246
Eco47III	AGCGCT	2 cut(s)	157, 1937
Eco57I	CTGAAG	1 cut(s)	1863
Eco88I	CYCGRG	2 cut(s)	53, 545
Eco91I	GGTNACC	1 cut(s)	423
EcoO109I	RGGNCCY	1 cut(s)	508
EcoO65I	GGTNACC	1 cut(s)	423
EcoRI	GAATTC	2 cut(s)	637, 1448
EcoRII	CCWGG	3 cut(s)	912, 1617, 2301
EcoT14I	CCWWGG	1 cut(s)	1993
EcoT22I	ATGCAT	3 cut(s)	6, 905, 1929
EcoT38I	GRGCYC	2 cut(s)	511, 2405
EgeI	GGCGCC	1 cut(s)	314
EheI	GGCGCC	1 cut(s)	314
ErhI	CCWWGG	1 cut(s)	1993
FaeI	CATG	7 cut(s)	594, 765, 903, 907, 1000, 1803, 2176
FalI	AAGNNNNNCTT	6 cut(s)	461, 493, 1262, 1294, 1424, 1456
FaqI	GGGAC	2 cut(s)	79, 1560
FatI	CATG	7 cut(s)	590, 761, 899, 903, 996, 1799, 2172
FauI	CCCGC	1 cut(s)	56
FauNDI	CATATG	1 cut(s)	2445
FblI	GTMKAC	3 cut(s)	572, 2029, 2392
FriOI	GRGCYC	2 cut(s)	511, 2405
FspBI	CTAG	2 cut(s)	953, 2168
GlaI	GCGC	4 cut(s)	157, 314, 349, 1937
GsaI	CCCAGC	1 cut(s)	1163
GsuI	CTGGAG	2 cut(s)	665, 2324
HaeII	RGCGCY	3 cut(s)	159, 316, 1939
HaeIII	GGCC	8 cut(s)	82, 306, 509, 621, 821, 989, 1562, 1773
HapII	CCGG	5 cut(s)	41, 263, 333, 366, 1942
HhaI	GCGC	4 cut(s)	158, 315, 350, 1938
Hin1I	GRCGYC	2 cut(s)	313, 2389
Hin1II	CATG	7 cut(s)	594, 765, 903, 907, 1000, 1803, 2176
Hin6I	GCGC	4 cut(s)	156, 313, 348, 1936
HinP1I	GCGC	4 cut(s)	156, 313, 348, 1936
HincII	GTYRAC	1 cut(s)	2393
HindII	GTYRAC	1 cut(s)	2393
HinfI	GANTC	8 cut(s)	185, 492, 647, 827, 1186, 1573, 1876, 2333
HpaII	CCGG	5 cut(s)	41, 263, 333, 366, 1942
HphI	GGTGA	6 cut(s)	305, 352, 1066, 1807, 2071, 2432
Hpy166II	GTNNAC	7 cut(s)	573, 691, 1168, 1201, 2030, 2039, 2393
Hpy8I	GTNNAC	7 cut(s)	573, 691, 1168, 1201, 2030, 2039, 2393
Hpy99I	CGWCG	3 cut(s)	401, 2391, 2394
HpyCH4III	ACNGT	6 cut(s)	583, 1027, 1421, 1627, 1661, 2266
HpyCH4IV	ACGT	5 cut(s)	932, 942, 1123, 1152, 2389
HpyCH4V	TGCA	8 cut(s)	4, 392, 691, 848, 903, 1464, 1895, 1927
HpyF10VI	GCNNNNNNNGC	9 cut(s)	79, 312, 342, 1115, 1779, 1924, 1933, 1952, 2315
HpyF3I	CTNAG	8 cut(s)	287, 468, 1239, 1441, 1963, 2091, 2450, 2466
HpySE526I	ACGT	5 cut(s)	932, 942, 1123, 1152, 2389
Hsp92I	GRCGYC	2 cut(s)	313, 2389
Hsp92II	CATG	7 cut(s)	594, 765, 903, 907, 1000, 1803, 2176
HspAI	GCGC	4 cut(s)	156, 313, 348, 1936
KasI	GGCGCC	1 cut(s)	312
KspI	CCGCGG	1 cut(s)	319
LmnI	GCTCC	5 cut(s)	1843, 1923, 2229, 2408, 2415
Lsp1109I	GCAGC	4 cut(s)	373, 376, 1143, 1925
LweI	GCATC	4 cut(s)	343, 507, 1914, 1974
MaeI	CTAG	2 cut(s)	953, 2168
MaeII	ACGT	5 cut(s)	932, 942, 1123, 1152, 2389
MaeIII	GTNAC	8 cut(s)	190, 197, 423, 596, 757, 1174, 1795, 2149
MfeI	CAATTG	1 cut(s)	623
MflI	RGATCY	4 cut(s)	43, 57, 2087, 2326
MhlI	GDGCHC	3 cut(s)	511, 693, 2405
MlsI	TGGCCA	2 cut(s)	621, 1773
MluNI	TGGCCA	2 cut(s)	621, 1773
Mly113I	GGCGCC	1 cut(s)	313
MlyI	GAGTC	4 cut(s)	194, 656, 1582, 2342
MmeI	TCCRAC	1 cut(s)	1107
Mox20I	TGGCCA	2 cut(s)	621, 1773
Mph1103I	ATGCAT	3 cut(s)	6, 905, 1929
MroXI	GAANNNNTTC	3 cut(s)	1277, 1562, 1875
MscI	TGGCCA	2 cut(s)	621, 1773
MseI	TTAA	4 cut(s)	26, 128, 753, 1904
Msp20I	TGGCCA	2 cut(s)	621, 1773
MspA1I	CMGCKG	4 cut(s)	291, 303, 318, 1396
MspI	CCGG	5 cut(s)	41, 263, 333, 366, 1942
MspR9I	CCNGG	5 cut(s)	333, 914, 1619, 1943, 2303
MunI	CAATTG	1 cut(s)	623
Mva1269I	GAATGC	1 cut(s)	927
MvaI	CCWGG	3 cut(s)	914, 1619, 2303
MvnI	CGCG	3 cut(s)	318, 348, 350
MwoI	GCNNNNNNNGC	9 cut(s)	79, 312, 342, 1115, 1779, 1924, 1933, 1952, 2315
NarI	GGCGCC	1 cut(s)	313
NciI	CCSGG	2 cut(s)	333, 1943
NdeI	CATATG	1 cut(s)	2445
NlaIII	CATG	7 cut(s)	594, 765, 903, 907, 1000, 1803, 2176
NlaIV	GGNNCC	9 cut(s)	67, 314, 509, 722, 1839, 1919, 1999, 2404, 2417
NmuCI	GTSAC	6 cut(s)	190, 197, 596, 757, 1174, 1795
NsiI	ATGCAT	3 cut(s)	6, 905, 1929
NspI	RCATGY	3 cut(s)	594, 765, 907
PaeI	GCATGC	1 cut(s)	907
PaeR7I	CTCGAG	1 cut(s)	545
PceI	AGGCCT	1 cut(s)	1562
PciI	ACATGT	1 cut(s)	590
PctI	GAATGC	1 cut(s)	927
PdmI	GAANNNNTTC	3 cut(s)	1277, 1562, 1875
PfeI	GAWTC	4 cut(s)	492, 827, 1186, 1876
Pfl23II	CGTACG	1 cut(s)	1567
PflFI	GACNNNGTC	1 cut(s)	581
PflMI	CCANNNNNTGG	1 cut(s)	1994
PfoI	TCCNGGA	1 cut(s)	2301
PleI	GAGTC	4 cut(s)	193, 655, 1581, 2341
PluTI	GGCGCC	1 cut(s)	316
PpsI	GAGTC	4 cut(s)	193, 655, 1581, 2341
Ppu21I	YACGTR	2 cut(s)	933, 1153
PscI	ACATGT	1 cut(s)	590
PshAI	GACNNNNGTC	1 cut(s)	184
PsiI	TTATAA	1 cut(s)	2261
Psp6I	CCWGG	3 cut(s)	912, 1617, 2301
PspEI	GGTNACC	1 cut(s)	423
PspFI	CCCAGC	1 cut(s)	1159
PspGI	CCWGG	3 cut(s)	912, 1617, 2301
PspLI	CGTACG	1 cut(s)	1567
PspN4I	GGNNCC	9 cut(s)	67, 314, 509, 722, 1839, 1919, 1999, 2404, 2417
PspOMI	GGGCCC	1 cut(s)	507
PspPI	GGNCC	4 cut(s)	66, 507, 508, 1246
PsrI	GAACNNNNNNTAC	2 cut(s)	704, 736
PstNI	CAGNNNCTG	1 cut(s)	439
PsuI	RGATCY	4 cut(s)	43, 57, 2087, 2326
PsyI	GACNNNGTC	1 cut(s)	581
PvuII	CAGCTG	1 cut(s)	1396
SacII	CCGCGG	1 cut(s)	319
SalI	GTCGAC	1 cut(s)	2391
SaqAI	TTAA	4 cut(s)	26, 128, 753, 1904
Sau96I	GGNCC	4 cut(s)	66, 507, 508, 1246
ScaI	AGTACT	2 cut(s)	473, 1663
SchI	GAGTC	4 cut(s)	194, 656, 1582, 2342
ScrFI	CCNGG	5 cut(s)	333, 914, 1619, 1943, 2303
SduI	GDGCHC	3 cut(s)	511, 693, 2405
SfaNI	GCATC	4 cut(s)	343, 507, 1914, 1974
SfcI	CTRYAG	1 cut(s)	565
SfoI	GGCGCC	1 cut(s)	314
Sfr274I	CTCGAG	1 cut(s)	545
Sfr303I	CCGCGG	1 cut(s)	319
SgrBI	CCGCGG	1 cut(s)	319
SinI	GGWCC	2 cut(s)	66, 1246
SlaI	CTCGAG	1 cut(s)	545
SmlI	CTYRAG	3 cut(s)	545, 1668, 2330
SmoI	CTYRAG	3 cut(s)	545, 1668, 2330
SphI	GCATGC	1 cut(s)	907
SseBI	AGGCCT	1 cut(s)	1562
SspDI	GGCGCC	1 cut(s)	312
SspMI	CTAG	2 cut(s)	953, 2168
StuI	AGGCCT	1 cut(s)	1562
StyD4I	CCNGG	5 cut(s)	331, 912, 1617, 1941, 2301
StyI	CCWWGG	1 cut(s)	1993
TaaI	ACNGT	6 cut(s)	583, 1027, 1421, 1627, 1661, 2266
TaiI	ACGT	5 cut(s)	935, 945, 1126, 1155, 2392
TaqI	TCGA	7 cut(s)	117, 135, 235, 399, 546, 2386, 2392
TaqII	GACCGA	1 cut(s)	83
TatI	WGTACW	6 cut(s)	471, 679, 1356, 1661, 1714, 2038
TauI	GCSGC	6 cut(s)	224, 306, 318, 321, 348, 467
TfiI	GAWTC	4 cut(s)	492, 827, 1186, 1876
Tru1I	TTAA	4 cut(s)	26, 128, 753, 1904
Tru9I	TTAA	4 cut(s)	26, 128, 753, 1904
TscAI	CASTG	2 cut(s)	586, 1426
TseFI	GTSAC	6 cut(s)	190, 197, 596, 757, 1174, 1795
TseI	GCWGC	4 cut(s)	386, 389, 1156, 1938
Tsp45I	GTSAC	6 cut(s)	190, 197, 596, 757, 1174, 1795
TspDTI	ATGAA	5 cut(s)	729, 862, 1515, 1527, 1591
TspGWI	ACGGA	1 cut(s)	1585
TspRI	CASTG	2 cut(s)	586, 1426
Tth111I	GACNNNGTC	1 cut(s)	581
Van91I	CCANNNNNTGG	1 cut(s)	1994
VneI	GTGCAC	1 cut(s)	689
VpaK11BI	GGWCC	2 cut(s)	66, 1246
XapI	RAATTY	6 cut(s)	637, 880, 1388, 1448, 1481, 2281
XceI	RCATGY	3 cut(s)	594, 765, 907
XhoI	CTCGAG	1 cut(s)	545
XmiI	GTMKAC	3 cut(s)	572, 2029, 2392
XmnI	GAANNNNTTC	3 cut(s)	1277, 1562, 1875
XspI	CTAG	2 cut(s)	953, 2168
ZraI	GACGTC	1 cut(s)	2390
ZrmI	AGTACT	2 cut(s)	473, 1663
Zsp2I	ATGCAT	3 cut(s)	6, 905, 1929

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.