Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0004348

Physical Location & Seq

Genomic Coordinates Forward (+)

45992952 .. 46007106

Gene Structure 14155 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG320700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2238 bp

ATGGAGGACCCATTGCTTAACGGAGAAGGCAGTAGTAGTCCCCGACGCGGCGGCGATTTGTCCAGGGCCAAGAGCTTCAAACGCCGCTCCGATGCCATCACCTTCGGCTCTTCTTATCAGAAAGCAGCCGCTCTCGTCGACTTGGCGGAAGACGGCATTGGCTTACCTGAGCAGATACTTGATCTCTCATCTTTCCATAGCTCTGCAAAATACTACTTCCTTTATATGCGATTCAGCTTCCTCTGGGCCCTACTCTACTTTGCCCTCTTGATTCTCAATTTCCTTGAGAAACCTCTGTGGTGTGCCAGGTATACTGATCATACTTGCAGTGACAGGGATTACTACTATCTCGGCCAATTGCCCTACTTGACTCCTACAGAGTCCCTTATTTATGAGGGCATAACACTGATTGTGCTCTTGGTGCATATTTTCTTTCCAATTTCATATGTGGGGTTCCGTCTCTATTGGAATAGTCCTCTTACTCAATTCAAGGTCATATGCCTGTTGATTTTGGTTGCTGATTTGCTGGCTTATGTTCTCTATTTGTCTCCAGTTGCCTTTGATTCTCTCCCTTTACGGGTTGCTCCATATATCAGAGTCATCTTTTTCATATTGAACTTTAGGGAATTACGATCGTGCATGCTCATCTTGGCTGGAATGTTTGGCACATACTTGAACGTCTTGGGTTTAGGGCTTTTATTTCTTCTGTTTTCCAGTTGGCTAGCTTATGTCATGTTTGAAGATACTGACCAGGGGACAATAGTATTTACTTCATATGGTACCACATTGTATCAGATGTTTGTTTTATTCACCACATCCAACAATCCAGATGTCTGGATACCTGCTTACAAGGCTTCACGTTGGTATTGCTTGTTTTTTGTTCTGTACGTGTTGCTAGGAGTCTATTTTGTCACAAATTTGGTCCTTGCCGTTGTGTATGACAGCTTCAAAAGTCAGCTTGTAAAGCAAGTTTCTGAGATGGACCAGACGAGGAAAACTATACTGAAGAAAGCCTTTCATTTAATAGAAGAAGATAATGCTGGATATATCAATAAGGACCAGTGCATACGTTTGTTTGAGGAACTAAACAAATACAGGACTTTGCCAAAAATATCAAGAGAAGAATTTGACTTGATATTTGATGAGCTGGATGACAGTGGAGACATCAAGATCAACTTAGATGAGTTCACTGACCTCTGTAACGCCATTGCCCTAAGATTCCAGAGGGAGGATACCCCGTCTTGGTTTGAAAAGTTCCCTTCGTGTTACCATTCTCCCTTCTCAGAGAAGTTGAAGGCCTTTATTCGGAGCCCCAAATTTGGATTCATAATATCCTTCATACTCATAATCAATCTATCTGCTGTTATCATTGAAACTACGCTTGACATAGAAAACAATTCTGCTCAGAAGGTATGGCAATCGATGGAGTTTGTCCTTGGGTGGGTATATGTACTGGAAATGGCTCTGAAAGTCTATTCATTTGGATTTGAGAACTATTGGAGGGATGGCCAAAATCGGTTTGATTTTCTGGTCACTTTGATAATAGCTATTGGAGAAACGCTAACTTTCGCCGCTCCAGAAGGACTAACTTTCCTTTCAAATGGAGAATGGATCCGCTACCTTCTCATTGCAAGGATGTTACGATTGATTAGGCTCCTGATGCATGTCCAGCGTTGCCGAGCCTTTATCGCCACATTCTTAACTCTTATTCCAAGTTTGATGCCATACCTTGGGACCATATTTTGTGTCATGTGCATCTATTGCACGCTTGGTGTACAGATCTTCGGCGGGATTGTTAATGCTGGAAGTGCCAATTTGGAAGAAACAGATCTTTATGACAACGACTATCTACTTTTCAACTTCAATGACTATCCAAATGGGATGGTGACACTTTTCAATTTACTAGTCACGGGAAACTGGCAAGATTGGATGCAGAGTTACAAGGTTTTAACGGGAACTTCTTGGAGTCTTCTATATTTTGTCAGTTTCTATCTTATAACCGTCTTACTTCTACTGAATTTGGTTGTGGCTTTTGTCCTGGAGGCGTTCTTTGCTGAAATGGATCTCGAGTCTGCAGAAATTTGTCAAAAACATGAGGAGGAGGTGGAAGGAGTAAAAGACCGACCTCGATCCGTAGGCTCAAAATCACGGAGCCAGAGATGCATAGCTTATGTTATTTTCCGTTATGGTTACGTTAGTGATTTGGTAGAACCAGAAAGAAGAGTGAAGGAAATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0001508 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005245 GO:0005261 GO:0005262 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008324 GO:0009791 GO:0009845 GO:0009889 GO:0009987 GO:0010119 GO:0010565 GO:0010817 GO:0012505 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019216 GO:0019217 GO:0019222 GO:0019722 GO:0019932 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0031090 GO:0031323 GO:0031326 GO:0032350 GO:0032501 GO:0032502 GO:0034220 GO:0035556 GO:0042304 GO:0042391 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046885 GO:0046890 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0051899 GO:0055085 GO:0062012 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0080090 GO:0080140 GO:0080141 GO:0086010 GO:0090351 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EF-hand_7 Ion_trans

Protein Analysis

745

Amino Acids

86.2

Weight (kDa)

5.14

Isoelectric Point (pI)

40.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	132 - 319	2.6e-16	Ion transport protein
EF-hand_7	PF13499	335 - 399	3.8e-07	EF-hand domain pair
Ion_trans	PF00520	440 - 684	3.9e-42	Ion transport protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004348)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G03560
fragaria_vesca	FvH4_6g26210 FvH4_6g26210 FvH4_6g26210 FvH4_6g26210
malus_domestica	MD09G1247300.v1.1 MD17G1240200.v1.1
prunus_persica	Prupe.3G138600_v2.0.a1 Prupe.3G138600_v2.0.a1 Prupe.3G138600_v2.0.a1
pyrus_communis	pycom09g16460 pycom17g24350
rosa_chinensis	RchiOBHm_Chr2g0126531 RchiOBHm_Chr2g0126551
rosa_laevigata	RLG00000018910 RLG00000018911
rosa_multiflora	Rmu_sc0002369.1_g000031 Rmu_sc0002369.1_g000036 Rmu_sc0007463.1_g000007
rosa_roxburghii	Rroxscaffold_2G00117090 Rroxscaffold_2G00117120
rosa_rugosa	Rorug02G0263600
rosa_samantha	Rh2AG320600 Rh2AG320700 Rh2BG329000 Rh2BG329100 Rh2CG308800 Rh2CG308900 Rh2DG348300 Rh2DG348400
rosa_wichuraiana	Rw2G025970 Rw2G025980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1997
Acc36I	ACCTGC	1 cut(s)	850
Acc65I	GGTACC	1 cut(s)	779
AccB1I	GGYRCC	1 cut(s)	779
AccB7I	CCANNNNNTGG	1 cut(s)	1730
AccBSI	CCGCTC	3 cut(s)	87, 131, 1574
AccI	GTMKAC	2 cut(s)	138, 311
AccII	CGCG	1 cut(s)	48
AciI	CCGC	8 cut(s)	48, 51, 85, 129, 146, 1572, 1615, 1788
AclWI	GGATC	4 cut(s)	1606, 1619, 2070, 2124
AcoI	YGGCCR	2 cut(s)	352, 1507
AcsI	RAATTY	5 cut(s)	916, 1124, 1316, 2017, 2079
AcuI	CTGAAG	1 cut(s)	1025
AfaI	GTAC	4 cut(s)	781, 887, 1452, 1776
AfiI	CCNNNNNNNGG	8 cut(s)	47, 577, 1228, 1242, 1305, 1319, 1441, 1730
AflIII	ACRYGT	1 cut(s)	888
AhlI	ACTAGT	1 cut(s)	1903
AjnI	CCWGG	4 cut(s)	62, 305, 750, 2037
AjuI	GAANNNNNNNTTGG	4 cut(s)	141, 173, 1307, 1339
AluBI	AGCT	9 cut(s)	75, 201, 237, 725, 945, 958, 1147, 1547, 2168
AluI	AGCT	9 cut(s)	75, 201, 237, 725, 945, 958, 1147, 1547, 2168
Alw21I	GWGCWC	1 cut(s)	417
Alw26I	GTCTC	3 cut(s)	464, 552, 1155
AlwI	GGATC	4 cut(s)	1606, 1619, 2070, 2124
Ama87I	CYCGRG	1 cut(s)	2066
AoxI	GGCC	5 cut(s)	66, 246, 352, 1296, 1507
ApaI	GGGCCC	1 cut(s)	250
ApeKI	GCWGC	1 cut(s)	125
ApoI	RAATTY	5 cut(s)	916, 1124, 1316, 2017, 2079
ArsI	GACNNNNNNTTYG	2 cut(s)	1121, 1153
Asp718I	GGTACC	1 cut(s)	779
AspS9I	GGNCC	8 cut(s)	7, 66, 246, 247, 922, 982, 1057, 1734
AsuHPI	GGTGA	3 cut(s)	91, 802, 1897
AsuNHI	GCTAGC	1 cut(s)	721
AvaI	CYCGRG	1 cut(s)	2066
AvaII	GGWCC	5 cut(s)	7, 922, 982, 1057, 1734
BaeGI	GKGCMC	1 cut(s)	250
BalI	TGGCCA	1 cut(s)	1509
BamHI	GGATCC	1 cut(s)	1611
BanI	GGYRCC	1 cut(s)	779
BanII	GRGCYC	2 cut(s)	250, 1313
BarI	GAAGNNNNNNTAC	2 cut(s)	929, 961
BbsI	GAAGAC	2 cut(s)	156, 1961
Bbv12I	GWGCWC	1 cut(s)	417
BbvI	GCAGC	1 cut(s)	137
BccI	CCATC	5 cut(s)	104, 973, 1417, 1499, 1876
BceAI	ACGGC	2 cut(s)	169, 914
BciT130I	CCWGG	4 cut(s)	64, 307, 752, 2039
BciVI	GTATCC	2 cut(s)	831, 1225
BclI	TGATCA	1 cut(s)	316
BcoDI	GTCTC	3 cut(s)	464, 552, 1155
BcuI	ACTAGT	1 cut(s)	1903
BfaI	CTAG	3 cut(s)	722, 896, 1904
BfmI	CTRYAG	2 cut(s)	375, 2073
BfuAI	ACCTGC	1 cut(s)	850
BfuI	GTATCC	2 cut(s)	831, 1225
BglII	AGATCT	2 cut(s)	1779, 1828
BisI	GCNGC	6 cut(s)	49, 52, 85, 126, 129, 1572
BlsI	GCNGC	6 cut(s)	50, 53, 86, 127, 130, 1573
Bme1390I	CCNGG	4 cut(s)	64, 307, 752, 2039
Bme18I	GGWCC	5 cut(s)	7, 922, 982, 1057, 1734
BmeT110I	CYCGRG	1 cut(s)	2066
BmgT120I	GGNCC	8 cut(s)	7, 66, 246, 247, 922, 982, 1057, 1734
BmiI	GGNNCC	9 cut(s)	9, 248, 455, 781, 1310, 1613, 1655, 1735, 2153
BmrFI	CCNGG	4 cut(s)	64, 307, 752, 2039
BmsI	GCATC	6 cut(s)	82, 1650, 1710, 1764, 1920, 2150
BmtI	GCTAGC	1 cut(s)	725
BpiI	GAAGAC	2 cut(s)	156, 1961
BpmI	CTGGAG	3 cut(s)	534, 1560, 2060
Bpu10I	CCTNAGC	1 cut(s)	168
BpuEI	CTTGAG	1 cut(s)	305
Bsa29I	ATCGAT	1 cut(s)	1421
BsaAI	YACGTR	1 cut(s)	889
BsaJI	CCNNGG	4 cut(s)	63, 751, 1435, 1729
Bsc4I	CCNNNNNNNGG	8 cut(s)	47, 577, 1228, 1242, 1305, 1319, 1441, 1730
Bse1I	ACTGG	5 cut(s)	551, 714, 1060, 1458, 1922
Bse3DI	GCAATG	3 cut(s)	11, 1206, 1626
BseBI	CCWGG	4 cut(s)	64, 307, 752, 2039
BseCI	ATCGAT	1 cut(s)	1421
BseDI	CCNNGG	4 cut(s)	63, 751, 1435, 1729
BseGI	GGATG	6 cut(s)	815, 1156, 1510, 1641, 1887, 1935
BseLI	CCNNNNNNNGG	8 cut(s)	47, 577, 1228, 1242, 1305, 1319, 1441, 1730
BseMI	GCAATG	3 cut(s)	11, 1206, 1626
BseMII	CTCAG	4 cut(s)	159, 966, 1296, 1418
BseNI	ACTGG	5 cut(s)	551, 714, 1060, 1458, 1922
BseRI	GAGGAG	2 cut(s)	2111, 2114
BseSI	GKGCMC	1 cut(s)	250
BseXI	GCAGC	1 cut(s)	137
Bsh1236I	CGCG	1 cut(s)	48
Bsh1285I	CGRYCG	1 cut(s)	635
BshFI	GGCC	5 cut(s)	68, 248, 354, 1298, 1509
BshNI	GGYRCC	1 cut(s)	779
BshVI	ATCGAT	1 cut(s)	1421
BsiEI	CGRYCG	1 cut(s)	635
BsiHKAI	GWGCWC	1 cut(s)	417
BsiHKCI	CYCGRG	1 cut(s)	2066
BslFI	GGGAC	4 cut(s)	24, 367, 769, 1747
BslI	CCNNNNNNNGG	8 cut(s)	47, 577, 1228, 1242, 1305, 1319, 1441, 1730
BsmAI	GTCTC	3 cut(s)	464, 552, 1155
BsmBI	CGTCTC	1 cut(s)	464
BsmFI	GGGAC	4 cut(s)	24, 367, 769, 1747
BsnI	GGCC	5 cut(s)	68, 248, 354, 1298, 1509
BsoBI	CYCGRG	1 cut(s)	2066
Bsp120I	GGGCCC	1 cut(s)	246
Bsp1286I	GDGCHC	3 cut(s)	250, 417, 1313
Bsp1407I	TGTACA	1 cut(s)	1774
Bsp143I	GATC	9 cut(s)	181, 316, 632, 1170, 1611, 1779, 1828, 2062, 2129
BspACI	CCGC	8 cut(s)	48, 51, 85, 129, 146, 1572, 1615, 1788
BspANI	GGCC	5 cut(s)	68, 248, 354, 1298, 1509
BspCNI	CTCAG	4 cut(s)	160, 967, 1295, 1417
BspDI	ATCGAT	1 cut(s)	1421
BspFNI	CGCG	1 cut(s)	48
BspLI	GGNNCC	9 cut(s)	9, 248, 455, 781, 1310, 1613, 1655, 1735, 2153
BspMAI	CTGCAG	1 cut(s)	2077
BspMI	ACCTGC	1 cut(s)	850
BspOI	GCTAGC	1 cut(s)	725
BspPI	GGATC	4 cut(s)	1606, 1619, 2070, 2124
BspQI	GCTCTTC	1 cut(s)	115
BspT107I	GGYRCC	1 cut(s)	779
BsrBI	CCGCTC	3 cut(s)	87, 131, 1574
BsrDI	GCAATG	3 cut(s)	11, 1206, 1626
BsrGI	TGTACA	1 cut(s)	1774
BsrI	ACTGG	5 cut(s)	551, 714, 1060, 1458, 1922
BssECI	CCNNGG	4 cut(s)	63, 751, 1435, 1729
BssMI	GATC	9 cut(s)	181, 316, 632, 1170, 1611, 1779, 1828, 2062, 2129
BssNAI	GTATAC	1 cut(s)	312
BssT1I	CCWWGG	2 cut(s)	1435, 1729
Bst1107I	GTATAC	1 cut(s)	312
Bst2UI	CCWGG	4 cut(s)	64, 307, 752, 2039
Bst4CI	ACNGT	2 cut(s)	1157, 2002
Bst6I	CTCTTC	2 cut(s)	115, 2215
BstAPI	GCANNNNNTGC	1 cut(s)	1761
BstAUI	TGTACA	1 cut(s)	1774
BstBAI	YACGTR	1 cut(s)	889
BstC8I	GCNNGC	4 cut(s)	528, 641, 723, 1766
BstDEI	CTNAG	6 cut(s)	168, 975, 1177, 1214, 1282, 1404
BstF5I	GGATG	6 cut(s)	815, 1156, 1510, 1641, 1887, 1935
BstFNI	CGCG	1 cut(s)	48
BstKTI	GATC	9 cut(s)	184, 319, 635, 1173, 1614, 1782, 1831, 2065, 2132
BstMAI	GTCTC	3 cut(s)	464, 552, 1155
BstMBI	GATC	9 cut(s)	181, 316, 632, 1170, 1611, 1779, 1828, 2062, 2129
BstMCI	CGRYCG	1 cut(s)	635
BstNI	CCWGG	4 cut(s)	64, 307, 752, 2039
BstNSI	RCATGY	2 cut(s)	643, 1667
BstSCI	CCNGG	4 cut(s)	62, 305, 750, 2037
BstSFI	CTRYAG	2 cut(s)	375, 2073
BstSLI	GKGCMC	1 cut(s)	250
BstUI	CGCG	1 cut(s)	48
BstV1I	GCAGC	1 cut(s)	137
BstV2I	GAAGAC	2 cut(s)	156, 1961
BstX2I	RGATCY	4 cut(s)	1611, 1779, 1828, 2062
BstXI	CCANNNNNNTGG	1 cut(s)	834
BstYI	RGATCY	4 cut(s)	1611, 1779, 1828, 2062
BstZ17I	GTATAC	1 cut(s)	312
Bsu15I	ATCGAT	1 cut(s)	1421
BsuI	GTATCC	2 cut(s)	831, 1225
BsuRI	GGCC	5 cut(s)	68, 248, 354, 1298, 1509
BsuTUI	ATCGAT	1 cut(s)	1421
BtsCI	GGATG	6 cut(s)	815, 1156, 1510, 1641, 1887, 1935
BtsI	GCAGTG	1 cut(s)	334
BtsIMutI	CAGTG	5 cut(s)	334, 404, 1067, 1162, 1188
BveI	ACCTGC	1 cut(s)	850
Cac8I	GCNNGC	4 cut(s)	528, 641, 723, 1766
Cfr13I	GGNCC	8 cut(s)	7, 66, 246, 247, 922, 982, 1057, 1734
ClaI	ATCGAT	1 cut(s)	1421
CseI	GACGC	1 cut(s)	54
Csp6I	GTAC	4 cut(s)	780, 886, 1451, 1775
CviAII	CATG	5 cut(s)	640, 733, 1664, 1750, 2093
CviQI	GTAC	4 cut(s)	780, 886, 1451, 1775
DdeI	CTNAG	6 cut(s)	168, 975, 1177, 1214, 1282, 1404
DpnI	GATC	9 cut(s)	183, 318, 634, 1172, 1613, 1781, 1830, 2064, 2131
DpnII	GATC	9 cut(s)	181, 316, 632, 1170, 1611, 1779, 1828, 2062, 2129
EaeI	YGGCCR	2 cut(s)	352, 1507
Eam1104I	CTCTTC	2 cut(s)	115, 2215
EarI	CTCTTC	2 cut(s)	115, 2215
EciI	GGCGGA	1 cut(s)	161
Eco130I	CCWWGG	2 cut(s)	1435, 1729
Eco147I	AGGCCT	1 cut(s)	1298
Eco24I	GRGCYC	2 cut(s)	250, 1313
Eco47I	GGWCC	5 cut(s)	7, 922, 982, 1057, 1734
Eco57I	CTGAAG	1 cut(s)	1025
Eco88I	CYCGRG	1 cut(s)	2066
EcoO109I	RGGNCCY	2 cut(s)	7, 247
EcoRII	CCWGG	4 cut(s)	62, 305, 750, 2037
EcoT14I	CCWWGG	2 cut(s)	1435, 1729
EcoT22I	ATGCAT	2 cut(s)	1665, 2165
EcoT38I	GRGCYC	2 cut(s)	250, 1313
ErhI	CCWWGG	2 cut(s)	1435, 1729
Esp3I	CGTCTC	1 cut(s)	464
FaeI	CATG	5 cut(s)	643, 736, 1667, 1753, 2096
FalI	AAGNNNNNCTT	4 cut(s)	998, 1030, 1160, 1192
FaqI	GGGAC	4 cut(s)	24, 367, 769, 1747
FatI	CATG	5 cut(s)	639, 732, 1663, 1749, 2092
FauI	CCCGC	1 cut(s)	1781
FauNDI	CATATG	3 cut(s)	445, 497, 775
FbaI	TGATCA	1 cut(s)	316
FblI	GTMKAC	2 cut(s)	138, 311
Fnu4HI	GCNGC	6 cut(s)	49, 52, 85, 126, 129, 1572
FokI	GGATG	6 cut(s)	802, 1163, 1517, 1648, 1894, 1942
FriOI	GRGCYC	2 cut(s)	250, 1313
Fsp4HI	GCNGC	6 cut(s)	49, 52, 85, 126, 129, 1572
FspBI	CTAG	3 cut(s)	722, 896, 1904
GluI	GCNGC	6 cut(s)	49, 52, 85, 126, 129, 1572
GsuI	CTGGAG	3 cut(s)	534, 1560, 2060
HaeIII	GGCC	5 cut(s)	68, 248, 354, 1298, 1509
HgaI	GACGC	1 cut(s)	54
Hin1II	CATG	5 cut(s)	643, 736, 1667, 1753, 2096
HincII	GTYRAC	1 cut(s)	139
HindII	GTYRAC	1 cut(s)	139
HphI	GGTGA	3 cut(s)	91, 802, 1897
Hpy166II	GTNNAC	4 cut(s)	139, 312, 1188, 1775
Hpy188I	TCNGA	9 cut(s)	91, 120, 596, 795, 976, 1285, 1308, 1407, 1467
Hpy188III	TCNNGA	9 cut(s)	268, 827, 835, 1116, 1168, 1222, 1577, 1657, 2066
Hpy8I	GTNNAC	4 cut(s)	139, 312, 1188, 1775
Hpy99I	CGWCG	2 cut(s)	48, 140
HpyCH4III	ACNGT	2 cut(s)	1157, 2002
HpyCH4IV	ACGT	5 cut(s)	678, 859, 888, 1069, 2193
HpyF3I	CTNAG	6 cut(s)	168, 975, 1177, 1214, 1282, 1404
HpySE526I	ACGT	5 cut(s)	678, 859, 888, 1069, 2193
Hsp92II	CATG	5 cut(s)	643, 736, 1667, 1753, 2096
KpnI	GGTACC	1 cut(s)	783
Ksp22I	TGATCA	1 cut(s)	316
Kzo9I	GATC	9 cut(s)	181, 316, 632, 1170, 1611, 1779, 1828, 2062, 2129
LguI	GCTCTTC	1 cut(s)	115
LmnI	GCTCC	6 cut(s)	92, 589, 1308, 1579, 1659, 2151
Lsp1109I	GCAGC	1 cut(s)	137
LweI	GCATC	6 cut(s)	82, 1650, 1710, 1764, 1920, 2150
MaeI	CTAG	3 cut(s)	722, 896, 1904
MaeII	ACGT	5 cut(s)	678, 859, 888, 1069, 2193
MalI	GATC	9 cut(s)	183, 318, 634, 1172, 1613, 1781, 1830, 2064, 2131
MbiI	CCGCTC	3 cut(s)	87, 131, 1574
MboI	GATC	9 cut(s)	181, 316, 632, 1170, 1611, 1779, 1828, 2062, 2129
MfeI	CAATTG	1 cut(s)	356
MflI	RGATCY	4 cut(s)	1611, 1779, 1828, 2062
MhlI	GDGCHC	3 cut(s)	250, 417, 1313
MlsI	TGGCCA	1 cut(s)	1509
MluNI	TGGCCA	1 cut(s)	1509
MlyI	GAGTC	6 cut(s)	364, 389, 606, 909, 1975, 2078
MmeI	TCCRAC	1 cut(s)	843
Mox20I	TGGCCA	1 cut(s)	1509
Mph1103I	ATGCAT	2 cut(s)	1665, 2165
MscI	TGGCCA	1 cut(s)	1509
MseI	TTAA	5 cut(s)	18, 1022, 1700, 1797, 1949
Msp20I	TGGCCA	1 cut(s)	1509
MspR9I	CCNGG	4 cut(s)	64, 307, 752, 2039
MunI	CAATTG	1 cut(s)	356
MvaI	CCWGG	4 cut(s)	64, 307, 752, 2039
MvnI	CGCG	1 cut(s)	48
NdeI	CATATG	3 cut(s)	445, 497, 775
NdeII	GATC	9 cut(s)	181, 316, 632, 1170, 1611, 1779, 1828, 2062, 2129
NheI	GCTAGC	1 cut(s)	721
NlaIII	CATG	5 cut(s)	643, 736, 1667, 1753, 2096
NlaIV	GGNNCC	9 cut(s)	9, 248, 455, 781, 1310, 1613, 1655, 1735, 2153
NmeAIII	GCCGAG	2 cut(s)	330, 1703
NmuCI	GTSAC	5 cut(s)	329, 910, 1531, 1885, 1906
NsiI	ATGCAT	2 cut(s)	1665, 2165
NspI	RCATGY	2 cut(s)	643, 1667
PaeI	GCATGC	1 cut(s)	643
PaeR7I	CTCGAG	1 cut(s)	2066
PceI	AGGCCT	1 cut(s)	1298
PciSI	GCTCTTC	1 cut(s)	115
PfeI	GAWTC	5 cut(s)	231, 271, 563, 1218, 1323
PflMI	CCANNNNNTGG	1 cut(s)	1730
PfoI	TCCNGGA	1 cut(s)	2037
PkrI	GCNGC	6 cut(s)	50, 53, 86, 127, 130, 1573
Ple19I	CGATCG	1 cut(s)	635
PleI	GAGTC	6 cut(s)	364, 388, 605, 908, 1974, 2077
PpsI	GAGTC	6 cut(s)	364, 388, 605, 908, 1974, 2077
Ppu21I	YACGTR	1 cut(s)	889
PpuMI	RGGWCCY	1 cut(s)	7
PsiI	TTATAA	1 cut(s)	1997
Psp5II	RGGWCCY	1 cut(s)	7
Psp6I	CCWGG	4 cut(s)	62, 305, 750, 2037
PspGI	CCWGG	4 cut(s)	62, 305, 750, 2037
PspN4I	GGNNCC	9 cut(s)	9, 248, 455, 781, 1310, 1613, 1655, 1735, 2153
PspOMI	GGGCCC	1 cut(s)	246
PspPI	GGNCC	8 cut(s)	7, 66, 246, 247, 922, 982, 1057, 1734
PspPPI	RGGWCCY	1 cut(s)	7
PstI	CTGCAG	1 cut(s)	2077
PsuI	RGATCY	4 cut(s)	1611, 1779, 1828, 2062
PvuI	CGATCG	1 cut(s)	635
RsaI	GTAC	4 cut(s)	781, 887, 1452, 1776
RsaNI	GTAC	4 cut(s)	780, 886, 1451, 1775
SalI	GTCGAC	1 cut(s)	137
SapI	GCTCTTC	1 cut(s)	115
SaqAI	TTAA	5 cut(s)	18, 1022, 1700, 1797, 1949
SatI	GCNGC	6 cut(s)	49, 52, 85, 126, 129, 1572
Sau3AI	GATC	9 cut(s)	181, 316, 632, 1170, 1611, 1779, 1828, 2062, 2129
Sau96I	GGNCC	8 cut(s)	7, 66, 246, 247, 922, 982, 1057, 1734
SchI	GAGTC	6 cut(s)	364, 389, 606, 909, 1975, 2078
ScrFI	CCNGG	4 cut(s)	64, 307, 752, 2039
SduI	GDGCHC	3 cut(s)	250, 417, 1313
SfaNI	GCATC	6 cut(s)	82, 1650, 1710, 1764, 1920, 2150
SfcI	CTRYAG	2 cut(s)	375, 2073
Sfr274I	CTCGAG	1 cut(s)	2066
SinI	GGWCC	5 cut(s)	7, 922, 982, 1057, 1734
SlaI	CTCGAG	1 cut(s)	2066
SmlI	CTYRAG	2 cut(s)	284, 2066
SmoI	CTYRAG	2 cut(s)	284, 2066
SpeI	ACTAGT	1 cut(s)	1903
SphI	GCATGC	1 cut(s)	643
SseBI	AGGCCT	1 cut(s)	1298
SsiI	CCGC	8 cut(s)	48, 51, 85, 129, 146, 1572, 1615, 1788
SspMI	CTAG	3 cut(s)	722, 896, 1904
StuI	AGGCCT	1 cut(s)	1298
StyD4I	CCNGG	4 cut(s)	62, 305, 750, 2037
StyI	CCWWGG	2 cut(s)	1435, 1729
TaaI	ACNGT	2 cut(s)	1157, 2002
TaiI	ACGT	5 cut(s)	681, 862, 891, 1072, 2196
TaqI	TCGA	4 cut(s)	138, 1421, 2067, 2128
TaqII	GACCGA	1 cut(s)	2136
TatI	WGTACW	2 cut(s)	1450, 1774
TauI	GCSGC	5 cut(s)	51, 54, 87, 131, 1574
TfiI	GAWTC	5 cut(s)	231, 271, 563, 1218, 1323
Tru1I	TTAA	5 cut(s)	18, 1022, 1700, 1797, 1949
Tru9I	TTAA	5 cut(s)	18, 1022, 1700, 1797, 1949
TscAI	CASTG	5 cut(s)	334, 411, 1067, 1162, 1195
TseFI	GTSAC	5 cut(s)	329, 910, 1531, 1885, 1906
TseI	GCWGC	1 cut(s)	125
Tsp45I	GTSAC	5 cut(s)	329, 910, 1531, 1885, 1906
TspDTI	ATGAA	7 cut(s)	432, 598, 762, 1007, 1315, 1327, 1467
TspGWI	ACGGA	5 cut(s)	36, 446, 2122, 2164, 2171
TspRI	CASTG	5 cut(s)	334, 411, 1067, 1162, 1195
Van91I	CCANNNNNTGG	1 cut(s)	1730
VpaK11BI	GGWCC	5 cut(s)	7, 922, 982, 1057, 1734
XapI	RAATTY	5 cut(s)	916, 1124, 1316, 2017, 2079
XceI	RCATGY	2 cut(s)	643, 1667
XhoI	CTCGAG	1 cut(s)	2066
XmiI	GTMKAC	2 cut(s)	138, 311
XspI	CTAG	3 cut(s)	722, 896, 1904
Zsp2I	ATGCAT	2 cut(s)	1665, 2165

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.