Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0004348

Physical Location & Seq

Genomic Coordinates Reverse (-)

44550048 .. 44564409

Gene Structure 14362 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00117090.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2190 bp

ATGGAGGACCCATTGCTTAACGGAGAAGGCAGTAATCCCCGACGCGGCGGCGATTTGTCCAGGGCCAAGAGCTTCAAACGCCGCTCCGATGCCATCACATCCGGCTCTTCTTATCAGAAAGCAGCTGCTCTCGTCGATTTGGCGGAAGACGGCGTTGGCTTACCTGAGCAGATACTTGATCTCTCATCTTTTCAGAGTTCTGCCAAGTACTACTTCCTTTATATGCGATTCAGCTTCCTTTGGGCCCTGCTCTACTTCGCCCTCTTGATTCTCAATTTCCTTGAGAAACCTCTGTGGTGTGCCAGGTATACTGATCATACTTGCAGTGAAAGGGATTACTACTATCTCGGCCAATTGCCCTACTTGACTCCTACAGAGTCCCTTATTTATGAGGGCATAACACTGATTGTACTCCTGGTGCATATTTTCTTTCCAATTTCATATGTGGGGTTCCGTCTCTATTGGAATAGTCCTCTTACTCAATTCAAGGTCATATGCCTGTTGATTTTGGTTGCTGATTTGCTGGCTTATGTTCTCTATTTGTCTCCAGTTGCCTTTGATTCTCTCCCTTTACGGGTTGCTCCATATATCAGAGTCATCTTTTTCATATTGAACTTTAGGGAATTACGATCGTGCATGCTCATCTTGGCTGGAATGTTTGGCACATACTTGAACGTCTTGGGTTTAGGGCTTTTATTTCTTCTGTTTTCCAGTTGGCTAGCTTATGTCATGTTTGAAGATACTGACCAGGGGACAATAGTATTTACTTCATATGGTACCACATTATATCAGATGTTTGTTTTATTCACCACATCCAACAATCCAGATGTCTGGATACCAGCCTACAAGGCTTCACGTTGGTATTGCTTGTTTTTTGTTCTGTACGTGTTGCTAGGAGTCTATTTTGTCACAAATTTGATCCTTGCCGTCGTGTATGACAGTTTCAAAAGTCAGCTTGTAAAGCAAGTTTCTGGGATGGACCAGACGAGGAAAACTATACTGAAGAAAGCCTTTCATTTAATAGAAAAGGATAATGCTGGATATATCAATAAGGACCAGTGCATACGTTTGTTTGTGGAACTGAACAAATACAGGACTTTGCCAAAAATATCAAGAGAAGAATTTGAGCTGATATTTAATGAGCTGGATGACAATTTCAGGCAGCCTATCAACTTAGATGAATTCACTGACCTCTGTAACGCCATTGCCCTAAGATTCCAGAAGGAGGATACTCCATCTTGGTTTGAAAAGTTCCCTTCGTTTTACCATTCTCCGTTCTCAGAGAAGTTGAAGGCCTTTATTCGGAGCCCCAAATTTGGATACATAATATCCTTCATACTCATAATCAATCTATCTGCTGTTATCGTTGAAACTACGCTTGACATAGAAAACAATTCTGCTCAGAAGGTATGGCAATCAATGGAGTTTATCCTTGGGTGGGTATATGTACTGGAAATGGCTCTGAAAGTCTATTCATTTGGATTTGAGAACTATTGGAGAGATGGCCAAAATCGGTTCGATTTTCTGGTCACATTGATAATAGCTATTGGAGAAACGCTAACTTTTGCTGCTCCAGAAGGACTAACTTTCCTTACAAACGGAGAATGGATTCGCTACCTTCTCATTGCAAGGATGTTACGATTGATTCGGCTCCTGATGCATGTCCAGCGTTGCCGAGCCTTTATCGCCACATTCTTAACTCTTATACCAAGTTTGATGCCATACCTTGGCACCATATTTTGTGTCATGAACATCTATTGTACGCTTGGTGTACAGTTCTCATGCCAAGTTTCGATGACATACAATGGGTCTATTTTGTGTTATGTTTATGGTAAGATAGATTATGCAGTTGGTGTACAGATCTTCGGCGGGATTGTTAATGCTGGAAATGCCAATTTGGAAGCAACAGATCTTTATGACAACGAAATACTTGTCAATACCACAGCAATACCACAGCTTTTAAAGAAGGCCTTTAAAGAATGCCTTTCTGTTGTGGCTTTTGTCCTGGAGGCTTTCTTTGCTGAAATGGATCTCGAGTCTGCAGAAATTTGTGAAAAACATGAGGAGGTGGAACTTGTAAAAGGCCGACATCGATCCGTAGGGTCAAAATCACGGAGCCAGAGAGTTGATGCTCTTCTTCATCATATGTTGAGTGCAGAGCTAAATGAAACGCAGTCCTCCAGTGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0001508 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005245 GO:0005261 GO:0005262 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008324 GO:0009791 GO:0009845 GO:0009889 GO:0009987 GO:0010119 GO:0010565 GO:0010817 GO:0012505 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019216 GO:0019217 GO:0019222 GO:0019722 GO:0019932 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0031090 GO:0031323 GO:0031326 GO:0032350 GO:0032501 GO:0032502 GO:0034220 GO:0035556 GO:0042304 GO:0042391 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046885 GO:0046890 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0051899 GO:0055085 GO:0062012 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0080090 GO:0080140 GO:0080141 GO:0086010 GO:0090351 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EF-hand_7 Ion_trans

Protein Analysis

729

Amino Acids

83.74

Weight (kDa)

5.76

Isoelectric Point (pI)

38.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	131 - 318	3.1e-16	Ion transport protein
EF-hand_7	PF13499	334 - 398	2.5e-06	EF-hand domain pair
Ion_trans	PF00520	439 - 594	1e-21	Ion transport protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004348)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G03560
fragaria_vesca	FvH4_6g26210 FvH4_6g26210 FvH4_6g26210 FvH4_6g26210
malus_domestica	MD09G1247300.v1.1 MD17G1240200.v1.1
prunus_persica	Prupe.3G138600_v2.0.a1 Prupe.3G138600_v2.0.a1 Prupe.3G138600_v2.0.a1
pyrus_communis	pycom09g16460 pycom17g24350
rosa_chinensis	RchiOBHm_Chr2g0126531 RchiOBHm_Chr2g0126551
rosa_laevigata	RLG00000018910 RLG00000018911
rosa_multiflora	Rmu_sc0002369.1_g000031 Rmu_sc0002369.1_g000036 Rmu_sc0007463.1_g000007
rosa_roxburghii	Rroxscaffold_2G00117090 Rroxscaffold_2G00117120
rosa_rugosa	Rorug02G0263600
rosa_samantha	Rh2AG320600 Rh2AG320700 Rh2BG329000 Rh2BG329100 Rh2CG308800 Rh2CG308900 Rh2DG348300 Rh2DG348400
rosa_wichuraiana	Rw2G025970 Rw2G025980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	776
AccB1I	GGYRCC	2 cut(s)	776, 1730
AccB7I	CCANNNNNTGG	1 cut(s)	1727
AccBSI	CCGCTC	1 cut(s)	84
AccI	GTMKAC	1 cut(s)	308
AccII	CGCG	1 cut(s)	45
AciI	CCGC	5 cut(s)	45, 48, 82, 143, 1869
AclWI	GGATC	3 cut(s)	913, 2037, 2088
AcoI	YGGCCR	2 cut(s)	349, 1504
AcsI	RAATTY	5 cut(s)	913, 1121, 1181, 1313, 2046
AcuI	CTGAAG	1 cut(s)	1022
AfaI	GTAC	8 cut(s)	209, 411, 778, 884, 1449, 1762, 1773, 1857
AfiI	CCNNNNNNNGG	7 cut(s)	44, 574, 1225, 1302, 1316, 1438, 1727
AflIII	ACRYGT	1 cut(s)	885
AgsI	TTSAA	9 cut(s)	76, 487, 613, 673, 737, 946, 1247, 1291, 1370
AjnI	CCWGG	5 cut(s)	59, 302, 414, 747, 2004
AjuI	GAANNNNNNNTTGG	4 cut(s)	138, 170, 197, 229
Alw26I	GTCTC	2 cut(s)	461, 549
AlwI	GGATC	3 cut(s)	913, 2037, 2088
Ama87I	CYCGRG	1 cut(s)	2033
AoxI	GGCC	7 cut(s)	63, 243, 349, 1293, 1504, 1968, 2083
ApaI	GGGCCC	1 cut(s)	247
ApeKI	GCWGC	4 cut(s)	122, 125, 1162, 1568
ApoI	RAATTY	5 cut(s)	913, 1121, 1181, 1313, 2046
ArsI	GACNNNNNNTTYG	2 cut(s)	1917, 1949
Asp700I	GAANNNNTTC	1 cut(s)	1608
Asp718I	GGTACC	1 cut(s)	776
AspS9I	GGNCC	6 cut(s)	7, 63, 243, 244, 979, 1054
AsuHPI	GGTGA	1 cut(s)	799
AsuNHI	GCTAGC	1 cut(s)	718
AvaI	CYCGRG	1 cut(s)	2033
AvaII	GGWCC	3 cut(s)	7, 979, 1054
BaeGI	GKGCMC	1 cut(s)	247
BalI	TGGCCA	1 cut(s)	1506
BanI	GGYRCC	2 cut(s)	776, 1730
BanII	GRGCYC	2 cut(s)	247, 1310
BbsI	GAAGAC	1 cut(s)	153
BbvI	GCAGC	4 cut(s)	112, 134, 1174, 1555
BccI	CCATC	4 cut(s)	101, 970, 1243, 1496
BceAI	ACGGC	2 cut(s)	166, 911
BciT130I	CCWGG	5 cut(s)	61, 304, 416, 749, 2006
BciVI	GTATCC	3 cut(s)	828, 1222, 1313
BclI	TGATCA	1 cut(s)	313
BcoDI	GTCTC	2 cut(s)	461, 549
BfaI	CTAG	2 cut(s)	719, 893
BfmI	CTRYAG	2 cut(s)	372, 2040
BfuI	GTATCC	3 cut(s)	828, 1222, 1313
BglI	GCCNNNNNGGC	1 cut(s)	848
BglII	AGATCT	2 cut(s)	1860, 1909
BisI	GCNGC	7 cut(s)	46, 49, 82, 123, 126, 1163, 1569
BlsI	GCNGC	7 cut(s)	47, 50, 83, 124, 127, 1164, 1570
BmcAI	AGTACT	1 cut(s)	209
Bme1390I	CCNGG	5 cut(s)	61, 304, 416, 749, 2006
Bme18I	GGWCC	3 cut(s)	7, 979, 1054
BmeT110I	CYCGRG	1 cut(s)	2033
BmgT120I	GGNCC	6 cut(s)	7, 63, 243, 244, 979, 1054
BmiI	GGNNCC	8 cut(s)	9, 245, 452, 778, 1307, 1652, 1732, 2117
BmrFI	CCNGG	5 cut(s)	61, 304, 416, 749, 2006
BmsI	GCATC	4 cut(s)	79, 1647, 1707, 2119
BmtI	GCTAGC	1 cut(s)	722
BpiI	GAAGAC	1 cut(s)	153
BpmI	CTGGAG	4 cut(s)	531, 1557, 2027, 2164
Bpu10I	CCTNAGC	1 cut(s)	165
BpuEI	CTTGAG	1 cut(s)	302
Bsa29I	ATCGAT	1 cut(s)	2092
BsaAI	YACGTR	1 cut(s)	886
BsaJI	CCNNGG	4 cut(s)	60, 748, 1432, 1726
Bsc4I	CCNNNNNNNGG	7 cut(s)	44, 574, 1225, 1302, 1316, 1438, 1727
Bse1I	ACTGG	5 cut(s)	548, 711, 1057, 1455, 2181
Bse3DI	GCAATG	3 cut(s)	11, 1203, 1623
BseBI	CCWGG	5 cut(s)	61, 304, 416, 749, 2006
BseCI	ATCGAT	1 cut(s)	2092
BseDI	CCNNGG	4 cut(s)	60, 748, 1432, 1726
BseGI	GGATG	5 cut(s)	98, 812, 981, 1153, 1638
BseLI	CCNNNNNNNGG	7 cut(s)	44, 574, 1225, 1302, 1316, 1438, 1727
BseMI	GCAATG	3 cut(s)	11, 1203, 1623
BseMII	CTCAG	3 cut(s)	156, 1293, 1415
BseNI	ACTGG	5 cut(s)	548, 711, 1057, 1455, 2181
BseRI	GAGGAG	1 cut(s)	2078
BseSI	GKGCMC	1 cut(s)	247
BseXI	GCAGC	4 cut(s)	112, 134, 1174, 1555
BsgI	GTGCAG	1 cut(s)	2175
Bsh1236I	CGCG	1 cut(s)	45
Bsh1285I	CGRYCG	1 cut(s)	632
BshFI	GGCC	7 cut(s)	65, 245, 351, 1295, 1506, 1970, 2085
BshNI	GGYRCC	2 cut(s)	776, 1730
BshVI	ATCGAT	1 cut(s)	2092
BsiEI	CGRYCG	1 cut(s)	632
BsiHKCI	CYCGRG	1 cut(s)	2033
BsiSI	CCGG	1 cut(s)	102
BslFI	GGGAC	2 cut(s)	364, 766
BslI	CCNNNNNNNGG	7 cut(s)	44, 574, 1225, 1302, 1316, 1438, 1727
BsmAI	GTCTC	2 cut(s)	461, 549
BsmBI	CGTCTC	1 cut(s)	461
BsmFI	GGGAC	2 cut(s)	364, 766
BsmI	GAATGC	1 cut(s)	1985
BsnI	GGCC	7 cut(s)	65, 245, 351, 1295, 1506, 1970, 2085
BsoBI	CYCGRG	1 cut(s)	2033
Bsp120I	GGGCCC	1 cut(s)	243
Bsp1286I	GDGCHC	2 cut(s)	247, 1310
Bsp1407I	TGTACA	2 cut(s)	1771, 1855
Bsp143I	GATC	8 cut(s)	178, 313, 629, 918, 1860, 1909, 2029, 2093
BspACI	CCGC	5 cut(s)	45, 48, 82, 143, 1869
BspANI	GGCC	7 cut(s)	65, 245, 351, 1295, 1506, 1970, 2085
BspCNI	CTCAG	3 cut(s)	157, 1292, 1414
BspDI	ATCGAT	1 cut(s)	2092
BspFNI	CGCG	1 cut(s)	45
BspHI	TCATGA	1 cut(s)	1746
BspLI	GGNNCC	8 cut(s)	9, 245, 452, 778, 1307, 1652, 1732, 2117
BspMAI	CTGCAG	1 cut(s)	2044
BspOI	GCTAGC	1 cut(s)	722
BspPI	GGATC	3 cut(s)	913, 2037, 2088
BspQI	GCTCTTC	2 cut(s)	112, 2139
BspT107I	GGYRCC	2 cut(s)	776, 1730
BsrBI	CCGCTC	1 cut(s)	84
BsrDI	GCAATG	3 cut(s)	11, 1203, 1623
BsrGI	TGTACA	2 cut(s)	1771, 1855
BsrI	ACTGG	5 cut(s)	548, 711, 1057, 1455, 2181
BssECI	CCNNGG	4 cut(s)	60, 748, 1432, 1726
BssMI	GATC	8 cut(s)	178, 313, 629, 918, 1860, 1909, 2029, 2093
BssNAI	GTATAC	1 cut(s)	309
BssT1I	CCWWGG	2 cut(s)	1432, 1726
Bst1107I	GTATAC	1 cut(s)	309
Bst2UI	CCWGG	5 cut(s)	61, 304, 416, 749, 2006
Bst4CI	ACNGT	2 cut(s)	941, 1776
Bst6I	CTCTTC	2 cut(s)	112, 2139
BstAUI	TGTACA	2 cut(s)	1771, 1855
BstBAI	YACGTR	1 cut(s)	886
BstC8I	GCNNGC	3 cut(s)	525, 638, 720
BstDEI	CTNAG	5 cut(s)	165, 1174, 1211, 1279, 1401
BstF5I	GGATG	5 cut(s)	98, 812, 981, 1153, 1638
BstFNI	CGCG	1 cut(s)	45
BstKTI	GATC	8 cut(s)	181, 316, 632, 921, 1863, 1912, 2032, 2096
BstMAI	GTCTC	2 cut(s)	461, 549
BstMBI	GATC	8 cut(s)	178, 313, 629, 918, 1860, 1909, 2029, 2093
BstMCI	CGRYCG	1 cut(s)	632
BstMWI	GCNNNNNNNGC	8 cut(s)	78, 848, 961, 1657, 1666, 1685, 1889, 2018
BstNI	CCWGG	5 cut(s)	61, 304, 416, 749, 2006
BstNSI	RCATGY	2 cut(s)	640, 1664
BstSCI	CCNGG	5 cut(s)	59, 302, 414, 747, 2004
BstSFI	CTRYAG	2 cut(s)	372, 2040
BstSLI	GKGCMC	1 cut(s)	247
BstUI	CGCG	1 cut(s)	45
BstV1I	GCAGC	4 cut(s)	112, 134, 1174, 1555
BstV2I	GAAGAC	1 cut(s)	153
BstX2I	RGATCY	3 cut(s)	1860, 1909, 2029
BstXI	CCANNNNNNTGG	1 cut(s)	831
BstYI	RGATCY	3 cut(s)	1860, 1909, 2029
BstZ17I	GTATAC	1 cut(s)	309
Bsu15I	ATCGAT	1 cut(s)	2092
BsuI	GTATCC	3 cut(s)	828, 1222, 1313
BsuRI	GGCC	7 cut(s)	65, 245, 351, 1295, 1506, 1970, 2085
BsuTUI	ATCGAT	1 cut(s)	2092
BtsCI	GGATG	5 cut(s)	98, 812, 981, 1153, 1638
BtsI	GCAGTG	1 cut(s)	331
BtsIMutI	CAGTG	5 cut(s)	331, 401, 1064, 1185, 2188
Cac8I	GCNNGC	3 cut(s)	525, 638, 720
CciI	TCATGA	1 cut(s)	1746
Cfr13I	GGNCC	6 cut(s)	7, 63, 243, 244, 979, 1054
ClaI	ATCGAT	1 cut(s)	2092
CseI	GACGC	1 cut(s)	51
Csp6I	GTAC	8 cut(s)	208, 410, 777, 883, 1448, 1761, 1772, 1856
CviAII	CATG	6 cut(s)	637, 730, 1661, 1747, 1782, 2060
CviQI	GTAC	8 cut(s)	208, 410, 777, 883, 1448, 1761, 1772, 1856
DdeI	CTNAG	5 cut(s)	165, 1174, 1211, 1279, 1401
DpnI	GATC	8 cut(s)	180, 315, 631, 920, 1862, 1911, 2031, 2095
DpnII	GATC	8 cut(s)	178, 313, 629, 918, 1860, 1909, 2029, 2093
DraI	TTTAAA	2 cut(s)	1962, 1975
EaeI	YGGCCR	2 cut(s)	349, 1504
Eam1104I	CTCTTC	2 cut(s)	112, 2139
EarI	CTCTTC	2 cut(s)	112, 2139
EciI	GGCGGA	1 cut(s)	158
Eco130I	CCWWGG	2 cut(s)	1432, 1726
Eco147I	AGGCCT	2 cut(s)	1295, 1970
Eco24I	GRGCYC	2 cut(s)	247, 1310
Eco47I	GGWCC	3 cut(s)	7, 979, 1054
Eco57I	CTGAAG	1 cut(s)	1022
Eco88I	CYCGRG	1 cut(s)	2033
EcoO109I	RGGNCCY	2 cut(s)	7, 244
EcoRI	GAATTC	1 cut(s)	1181
EcoRII	CCWGG	5 cut(s)	59, 302, 414, 747, 2004
EcoT14I	CCWWGG	2 cut(s)	1432, 1726
EcoT22I	ATGCAT	1 cut(s)	1662
EcoT38I	GRGCYC	2 cut(s)	247, 1310
ErhI	CCWWGG	2 cut(s)	1432, 1726
Esp3I	CGTCTC	1 cut(s)	461
FaeI	CATG	6 cut(s)	640, 733, 1664, 1750, 1785, 2063
FalI	AAGNNNNNCTT	8 cut(s)	197, 229, 995, 1027, 1955, 1987, 1968, 2000
FaqI	GGGAC	2 cut(s)	364, 766
FatI	CATG	6 cut(s)	636, 729, 1660, 1746, 1781, 2059
FauI	CCCGC	1 cut(s)	1862
FauNDI	CATATG	4 cut(s)	442, 494, 772, 2145
FbaI	TGATCA	1 cut(s)	313
FblI	GTMKAC	1 cut(s)	308
Fnu4HI	GCNGC	7 cut(s)	46, 49, 82, 123, 126, 1163, 1569
FokI	GGATG	5 cut(s)	85, 799, 988, 1160, 1645
FriOI	GRGCYC	2 cut(s)	247, 1310
Fsp4HI	GCNGC	7 cut(s)	46, 49, 82, 123, 126, 1163, 1569
FspBI	CTAG	2 cut(s)	719, 893
GluI	GCNGC	7 cut(s)	46, 49, 82, 123, 126, 1163, 1569
GsuI	CTGGAG	4 cut(s)	531, 1557, 2027, 2164
HaeIII	GGCC	7 cut(s)	65, 245, 351, 1295, 1506, 1970, 2085
HapII	CCGG	1 cut(s)	102
HgaI	GACGC	1 cut(s)	51
Hin1II	CATG	6 cut(s)	640, 733, 1664, 1750, 1785, 2063
HpaII	CCGG	1 cut(s)	102
HphI	GGTGA	1 cut(s)	799
Hpy166II	GTNNAC	3 cut(s)	309, 1772, 1856
Hpy188I	TCNGA	9 cut(s)	88, 117, 195, 593, 792, 1282, 1305, 1404, 1464
Hpy188III	TCNNGA	9 cut(s)	265, 824, 832, 1113, 1219, 1574, 1654, 1747, 2033
Hpy8I	GTNNAC	3 cut(s)	309, 1772, 1856
Hpy99I	CGWCG	3 cut(s)	45, 137, 932
HpyAV	CCTTC	9 cut(s)	20, 1216, 1266, 1285, 1342, 1399, 1571, 1628, 1960
HpyCH4III	ACNGT	2 cut(s)	941, 1776
HpyCH4IV	ACGT	4 cut(s)	675, 856, 885, 1066
HpyF10VI	GCNNNNNNNGC	8 cut(s)	78, 848, 961, 1657, 1666, 1685, 1889, 2018
HpyF3I	CTNAG	5 cut(s)	165, 1174, 1211, 1279, 1401
HpySE526I	ACGT	4 cut(s)	675, 856, 885, 1066
Hsp92II	CATG	6 cut(s)	640, 733, 1664, 1750, 1785, 2063
KpnI	GGTACC	1 cut(s)	780
Ksp22I	TGATCA	1 cut(s)	313
Kzo9I	GATC	8 cut(s)	178, 313, 629, 918, 1860, 1909, 2029, 2093
LguI	GCTCTTC	2 cut(s)	112, 2139
LmnI	GCTCC	6 cut(s)	89, 586, 1305, 1576, 1656, 2115
Lsp1109I	GCAGC	4 cut(s)	112, 134, 1174, 1555
LweI	GCATC	4 cut(s)	79, 1647, 1707, 2119
MaeI	CTAG	2 cut(s)	719, 893
MaeII	ACGT	4 cut(s)	675, 856, 885, 1066
MaeIII	GTNAC	4 cut(s)	907, 1196, 1528, 1635
MalI	GATC	8 cut(s)	180, 315, 631, 920, 1862, 1911, 2031, 2095
MbiI	CCGCTC	1 cut(s)	84
MboI	GATC	8 cut(s)	178, 313, 629, 918, 1860, 1909, 2029, 2093
MboII	GAAGA	9 cut(s)	99, 158, 692, 749, 1015, 1130, 1855, 2126, 2129
MfeI	CAATTG	1 cut(s)	353
MflI	RGATCY	3 cut(s)	1860, 1909, 2029
MhlI	GDGCHC	2 cut(s)	247, 1310
MlsI	TGGCCA	1 cut(s)	1506
MluNI	TGGCCA	1 cut(s)	1506
MlyI	GAGTC	5 cut(s)	361, 386, 603, 906, 2045
MmeI	TCCRAC	1 cut(s)	840
Mox20I	TGGCCA	1 cut(s)	1506
Mph1103I	ATGCAT	1 cut(s)	1662
MroXI	GAANNNNTTC	1 cut(s)	1608
MscI	TGGCCA	1 cut(s)	1506
MseI	TTAA	7 cut(s)	18, 1019, 1137, 1697, 1878, 1961, 1974
Msp20I	TGGCCA	1 cut(s)	1506
MspA1I	CMGCKG	1 cut(s)	125
MspI	CCGG	1 cut(s)	102
MspR9I	CCNGG	5 cut(s)	61, 304, 416, 749, 2006
MunI	CAATTG	1 cut(s)	353
Mva1269I	GAATGC	1 cut(s)	1985
MvaI	CCWGG	5 cut(s)	61, 304, 416, 749, 2006
MvnI	CGCG	1 cut(s)	45
MwoI	GCNNNNNNNGC	8 cut(s)	78, 848, 961, 1657, 1666, 1685, 1889, 2018
NdeI	CATATG	4 cut(s)	442, 494, 772, 2145
NdeII	GATC	8 cut(s)	178, 313, 629, 918, 1860, 1909, 2029, 2093
NheI	GCTAGC	1 cut(s)	718
NlaIII	CATG	6 cut(s)	640, 733, 1664, 1750, 1785, 2063
NlaIV	GGNNCC	8 cut(s)	9, 245, 452, 778, 1307, 1652, 1732, 2117
NmeAIII	GCCGAG	2 cut(s)	327, 1700
NmuCI	GTSAC	2 cut(s)	907, 1528
NsiI	ATGCAT	1 cut(s)	1662
NspI	RCATGY	2 cut(s)	640, 1664
PaeI	GCATGC	1 cut(s)	640
PaeR7I	CTCGAG	1 cut(s)	2033
PagI	TCATGA	1 cut(s)	1746
PceI	AGGCCT	2 cut(s)	1295, 1970
PciSI	GCTCTTC	2 cut(s)	112, 2139
PctI	GAATGC	1 cut(s)	1985
PdmI	GAANNNNTTC	1 cut(s)	1608
PfeI	GAWTC	6 cut(s)	228, 268, 560, 1215, 1609, 1645
PflMI	CCANNNNNTGG	1 cut(s)	1727
PfoI	TCCNGGA	1 cut(s)	2004
PkrI	GCNGC	7 cut(s)	47, 50, 83, 124, 127, 1164, 1570
Ple19I	CGATCG	1 cut(s)	632
PleI	GAGTC	5 cut(s)	361, 385, 602, 905, 2044
PpsI	GAGTC	5 cut(s)	361, 385, 602, 905, 2044
Ppu21I	YACGTR	1 cut(s)	886
PpuMI	RGGWCCY	1 cut(s)	7
Psp5II	RGGWCCY	1 cut(s)	7
Psp6I	CCWGG	5 cut(s)	59, 302, 414, 747, 2004
PspGI	CCWGG	5 cut(s)	59, 302, 414, 747, 2004
PspN4I	GGNNCC	8 cut(s)	9, 245, 452, 778, 1307, 1652, 1732, 2117
PspOMI	GGGCCC	1 cut(s)	243
PspPI	GGNCC	6 cut(s)	7, 63, 243, 244, 979, 1054
PspPPI	RGGWCCY	1 cut(s)	7
PstI	CTGCAG	1 cut(s)	2044
PsuI	RGATCY	3 cut(s)	1860, 1909, 2029
PvuI	CGATCG	1 cut(s)	632
PvuII	CAGCTG	1 cut(s)	125
RsaI	GTAC	8 cut(s)	209, 411, 778, 884, 1449, 1762, 1773, 1857
RsaNI	GTAC	8 cut(s)	208, 410, 777, 883, 1448, 1761, 1772, 1856
SapI	GCTCTTC	2 cut(s)	112, 2139
SaqAI	TTAA	7 cut(s)	18, 1019, 1137, 1697, 1878, 1961, 1974
SatI	GCNGC	7 cut(s)	46, 49, 82, 123, 126, 1163, 1569
Sau3AI	GATC	8 cut(s)	178, 313, 629, 918, 1860, 1909, 2029, 2093
Sau96I	GGNCC	6 cut(s)	7, 63, 243, 244, 979, 1054
ScaI	AGTACT	1 cut(s)	209
SchI	GAGTC	5 cut(s)	361, 386, 603, 906, 2045
ScrFI	CCNGG	5 cut(s)	61, 304, 416, 749, 2006
SduI	GDGCHC	2 cut(s)	247, 1310
SfaNI	GCATC	4 cut(s)	79, 1647, 1707, 2119
SfcI	CTRYAG	2 cut(s)	372, 2040
Sfr274I	CTCGAG	1 cut(s)	2033
SinI	GGWCC	3 cut(s)	7, 979, 1054
SlaI	CTCGAG	1 cut(s)	2033
SmlI	CTYRAG	2 cut(s)	281, 2033
SmoI	CTYRAG	2 cut(s)	281, 2033
SphI	GCATGC	1 cut(s)	640
SseBI	AGGCCT	2 cut(s)	1295, 1970
SsiI	CCGC	5 cut(s)	45, 48, 82, 143, 1869
SspMI	CTAG	2 cut(s)	719, 893
StuI	AGGCCT	2 cut(s)	1295, 1970
StyD4I	CCNGG	5 cut(s)	59, 302, 414, 747, 2004
StyI	CCWWGG	2 cut(s)	1432, 1726
TaaI	ACNGT	2 cut(s)	941, 1776
TaiI	ACGT	4 cut(s)	678, 859, 888, 1069
TaqI	TCGA	5 cut(s)	135, 1518, 1793, 2034, 2092
TatI	WGTACW	5 cut(s)	207, 409, 1447, 1771, 1855
TauI	GCSGC	3 cut(s)	48, 51, 84
TfiI	GAWTC	6 cut(s)	228, 268, 560, 1215, 1609, 1645
Tru1I	TTAA	7 cut(s)	18, 1019, 1137, 1697, 1878, 1961, 1974
Tru9I	TTAA	7 cut(s)	18, 1019, 1137, 1697, 1878, 1961, 1974
TscAI	CASTG	5 cut(s)	331, 408, 1064, 1192, 2188
TseFI	GTSAC	2 cut(s)	907, 1528
TseI	GCWGC	4 cut(s)	122, 125, 1162, 1568
Tsp45I	GTSAC	2 cut(s)	907, 1528
TspGWI	ACGGA	6 cut(s)	36, 443, 1263, 1614, 2086, 2128
TspRI	CASTG	5 cut(s)	331, 408, 1064, 1192, 2188
Van91I	CCANNNNNTGG	1 cut(s)	1727
VpaK11BI	GGWCC	3 cut(s)	7, 979, 1054
XapI	RAATTY	5 cut(s)	913, 1121, 1181, 1313, 2046
XceI	RCATGY	2 cut(s)	640, 1664
XhoI	CTCGAG	1 cut(s)	2033
XmiI	GTMKAC	1 cut(s)	308
XmnI	GAANNNNTTC	1 cut(s)	1608
XspI	CTAG	2 cut(s)	719, 893
ZrmI	AGTACT	1 cut(s)	209
Zsp2I	ATGCAT	1 cut(s)	1662

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.