MD17G1242500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0012548
Physical Location & Seq
Forward (+)
29157516 .. 29162646
5131 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1242500.v1.1.491

Sequence Viewer

Length: 678 bp
ATGGGAGGGGTGGCGAGGAGCTGCGTGCAATCATTGCTCAAGTTGGTGAATTGTATGATTGGGATGGTAGGGCTCGCCATGATTATGTACTCGTTGTGGTTGTTTAGCGCGTGGCAGAGAAACATGCCTCACTTCCCCCCACCACTCGACCACTCTCACCACCATCCTACACCTTGGTTCATATACACTTTTCTTGGCCTTGGGATCACTCTGCTTGTGATCACATGCTCAGGTCATATCGCCGCTGACACTGCAAATGGTTGTTGCCTTTATTTGTACATGGTGTTTGTCTTCTTACTCTTCATGCTTGAAGCTGGAGTGACTGCTGACATATTTTTAAACCGTGACTGGGAAGAGGACTTTCCAGATGATCCAACTGGGAGATTTGATCAATTCAAAGAATTTGTAAGGTCGAACTTTGATATCTGCAAATGGATTGGCTTGTCAATAGTTTGTGTACAGGGGCTGTCTTTGTTATTGGCAATGATACTCAAAGCTCTTGGGCCACATCCGTATTATGATAGTGATGATGAGTATATTCCTGAGAGGGTTCCACTCCTAAAGAATGCTGTTCTTCCACCTCCTTATGTCGTTGGTGACCCTGTATATGGTTCCAATGTATATGGTTCCAAAAGTGATTCATGGAACATAAGGATCAATGACAAGACAAACAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

226

Amino Acids

25.45

Weight (kDa)

5.55

Isoelectric Point (pI)

35.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 11 - 117 1.6e-11 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 110
AciI CCGC 1 cut(s) 243
AclWI GGATC 3 cut(s) 212, 365, 662
AcsI RAATTY 1 cut(s) 401
AfaI GTAC 3 cut(s) 89, 278, 459
AfiI CCNNNNNNNGG 2 cut(s) 349, 608
AgsI TTSAA 2 cut(s) 311, 397
AluBI AGCT 3 cut(s) 21, 314, 497
AluI AGCT 3 cut(s) 21, 314, 497
AlwI GGATC 3 cut(s) 212, 365, 662
AlwNI CAGNNNCTG 1 cut(s) 466
AoxI GGCC 2 cut(s) 196, 503
ApeKI GCWGC 1 cut(s) 21
ApoI RAATTY 1 cut(s) 401
AspLEI GCGC 1 cut(s) 110
AspS9I GGNCC 1 cut(s) 503
AsuHPI GGTGA 3 cut(s) 58, 149, 608
BanII GRGCYC 1 cut(s) 75
BbsI GAAGAC 1 cut(s) 283
BbvI GCAGC 1 cut(s) 8
BccI CCATC 2 cut(s) 58, 171
BclI TGATCA 2 cut(s) 219, 388
BisI GCNGC 2 cut(s) 22, 243
BlsI GCNGC 2 cut(s) 23, 244
BmgT120I GGNCC 1 cut(s) 503
BmiI GGNNCC 3 cut(s) 552, 613, 628
BmrI ACTGGG 2 cut(s) 358, 387
BmuI ACTGGG 2 cut(s) 358, 387
BpiI GAAGAC 1 cut(s) 283
BpmI CTGGAG 1 cut(s) 336
Bpu10I CCTNAGC 1 cut(s) 229
BpuEI CTTGAG 1 cut(s) 23
BsaJI CCNNGG 2 cut(s) 173, 199
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 608
Bse1I ACTGG 2 cut(s) 353, 382
Bse3DI GCAATG 2 cut(s) 32, 489
BseDI CCNNGG 2 cut(s) 173, 199
BseGI GGATG 3 cut(s) 69, 163, 508
BseLI CCNNNNNNNGG 2 cut(s) 349, 608
BseMI GCAATG 2 cut(s) 32, 489
BseMII CTCAG 2 cut(s) 243, 534
BseNI ACTGG 2 cut(s) 353, 382
BseRI GAGGAG 1 cut(s) 31
BseXI GCAGC 1 cut(s) 8
Bsh1236I CGCG 1 cut(s) 110
BshFI GGCC 2 cut(s) 198, 505
BslI CCNNNNNNNGG 2 cut(s) 349, 608
BsmI GAATGC 1 cut(s) 571
BsnI GGCC 2 cut(s) 198, 505
Bsp1286I GDGCHC 1 cut(s) 75
Bsp1407I TGTACA 2 cut(s) 276, 457
Bsp143I GATC 5 cut(s) 204, 219, 370, 388, 654
BspACI CCGC 1 cut(s) 243
BspANI GGCC 2 cut(s) 198, 505
BspCNI CTCAG 2 cut(s) 242, 535
BspFNI CGCG 1 cut(s) 110
BspLI GGNNCC 3 cut(s) 552, 613, 628
BspPI GGATC 3 cut(s) 212, 365, 662
BsrDI GCAATG 2 cut(s) 32, 489
BsrGI TGTACA 2 cut(s) 276, 457
BsrI ACTGG 2 cut(s) 353, 382
BssECI CCNNGG 2 cut(s) 173, 199
BssMI GATC 5 cut(s) 204, 219, 370, 388, 654
BssT1I CCWWGG 2 cut(s) 173, 199
Bst4CI ACNGT 1 cut(s) 344
Bst6I CTCTTC 2 cut(s) 305, 348
BstAPI GCANNNNNTGC 1 cut(s) 34
BstAUI TGTACA 2 cut(s) 276, 457
BstC8I GCNNGC 2 cut(s) 26, 75
BstDEI CTNAG 2 cut(s) 229, 543
BstEII GGTNACC 1 cut(s) 596
BstF5I GGATG 3 cut(s) 69, 163, 508
BstFNI CGCG 1 cut(s) 110
BstHHI GCGC 1 cut(s) 110
BstKTI GATC 5 cut(s) 207, 222, 373, 391, 657
BstMBI GATC 5 cut(s) 204, 219, 370, 388, 654
BstMWI GCNNNNNNNGC 2 cut(s) 34, 251
BstNSI RCATGY 2 cut(s) 127, 228
BstPI GGTNACC 1 cut(s) 596
BstUI CGCG 1 cut(s) 110
BstV1I GCAGC 1 cut(s) 8
BstV2I GAAGAC 1 cut(s) 283
BsuRI GGCC 2 cut(s) 198, 505
BtsCI GGATG 3 cut(s) 69, 163, 508
BtsI GCAGTG 1 cut(s) 249
BtsIMutI CAGTG 1 cut(s) 249
Cac8I GCNNGC 2 cut(s) 26, 75
CaiI CAGNNNCTG 1 cut(s) 466
CfoI GCGC 1 cut(s) 110
Cfr13I GGNCC 1 cut(s) 503
Csp6I GTAC 3 cut(s) 88, 277, 458
CviAII CATG 6 cut(s) 79, 124, 225, 280, 304, 642
CviJI RGCY 8 cut(s) 21, 73, 198, 314, 441, 466, 497, 505
CviKI_1 RGCY 8 cut(s) 21, 73, 198, 314, 441, 466, 497, 505
CviQI GTAC 3 cut(s) 88, 277, 458
DdeI CTNAG 2 cut(s) 229, 543
DpnI GATC 5 cut(s) 206, 221, 372, 390, 656
DpnII GATC 5 cut(s) 204, 219, 370, 388, 654
DraI TTTAAA 1 cut(s) 339
Eam1104I CTCTTC 2 cut(s) 305, 348
EarI CTCTTC 2 cut(s) 305, 348
Eco130I CCWWGG 2 cut(s) 173, 199
Eco24I GRGCYC 1 cut(s) 75
Eco32I GATATC 1 cut(s) 424
Eco91I GGTNACC 1 cut(s) 596
EcoO65I GGTNACC 1 cut(s) 596
EcoRV GATATC 1 cut(s) 424
EcoT14I CCWWGG 2 cut(s) 173, 199
EcoT38I GRGCYC 1 cut(s) 75
ErhI CCWWGG 2 cut(s) 173, 199
FaeI CATG 6 cut(s) 82, 127, 228, 283, 307, 645
FatI CATG 6 cut(s) 78, 123, 224, 279, 303, 641
FbaI TGATCA 2 cut(s) 219, 388
Fnu4HI GCNGC 2 cut(s) 22, 243
FokI GGATG 3 cut(s) 76, 150, 495
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 2 cut(s) 22, 243
GlaI GCGC 1 cut(s) 109
GluI GCNGC 2 cut(s) 22, 243
GsuI CTGGAG 1 cut(s) 336
HaeIII GGCC 2 cut(s) 198, 505
HhaI GCGC 1 cut(s) 110
Hin1II CATG 6 cut(s) 82, 127, 228, 283, 307, 645
Hin6I GCGC 1 cut(s) 108
HinP1I GCGC 1 cut(s) 108
HinfI GANTC 1 cut(s) 638
HphI GGTGA 3 cut(s) 58, 149, 608
Hpy166II GTNNAC 1 cut(s) 458
Hpy188III TCNNGA 2 cut(s) 365, 542
Hpy8I GTNNAC 1 cut(s) 458
HpyCH4III ACNGT 1 cut(s) 344
HpyCH4V TGCA 3 cut(s) 28, 254, 429
HpyF10VI GCNNNNNNNGC 2 cut(s) 34, 251
HpyF3I CTNAG 2 cut(s) 229, 543
Hsp92II CATG 6 cut(s) 82, 127, 228, 283, 307, 645
HspAI GCGC 1 cut(s) 108
Ksp22I TGATCA 2 cut(s) 219, 388
Kzo9I GATC 5 cut(s) 204, 219, 370, 388, 654
LmnI GCTCC 1 cut(s) 18
LpnPI CCDG 9 cut(s) 216, 300, 334, 363, 378, 446, 555, 615, 658
Lsp1109I GCAGC 1 cut(s) 8
MaeIII GTNAC 3 cut(s) 319, 344, 596
MalI GATC 5 cut(s) 206, 221, 372, 390, 656
MboI GATC 5 cut(s) 204, 219, 370, 388, 654
MboII GAAGA 4 cut(s) 283, 292, 365, 566
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 3 cut(s) 49, 392, 401
MmeI TCCRAC 1 cut(s) 398
MnlI CCTC 5 cut(s) 9, 138, 349, 540, 591
MseI TTAA 1 cut(s) 338
MslI CAYNNNNRTG 1 cut(s) 83
MspA1I CMGCKG 1 cut(s) 245
Mva1269I GAATGC 1 cut(s) 571
MvnI CGCG 1 cut(s) 110
MwoI GCNNNNNNNGC 2 cut(s) 34, 251
NdeII GATC 5 cut(s) 204, 219, 370, 388, 654
NlaIII CATG 6 cut(s) 82, 127, 228, 283, 307, 645
NlaIV GGNNCC 3 cut(s) 552, 613, 628
NmuCI GTSAC 3 cut(s) 319, 344, 596
NspI RCATGY 2 cut(s) 127, 228
PctI GAATGC 1 cut(s) 571
PfeI GAWTC 1 cut(s) 638
PkrI GCNGC 2 cut(s) 23, 244
PspEI GGTNACC 1 cut(s) 596
PspN4I GGNNCC 3 cut(s) 552, 613, 628
PspPI GGNCC 1 cut(s) 503
PstNI CAGNNNCTG 1 cut(s) 466
RsaI GTAC 3 cut(s) 89, 278, 459
RsaNI GTAC 3 cut(s) 88, 277, 458
RseI CAYNNNNRTG 1 cut(s) 83
SaqAI TTAA 1 cut(s) 338
SatI GCNGC 2 cut(s) 22, 243
Sau3AI GATC 5 cut(s) 204, 219, 370, 388, 654
Sau96I GGNCC 1 cut(s) 503
SduI GDGCHC 1 cut(s) 75
SetI ASST 8 cut(s) 23, 175, 235, 316, 413, 499, 583, 677
SmiMI CAYNNNNRTG 1 cut(s) 83
SmlI CTYRAG 1 cut(s) 38
SmoI CTYRAG 1 cut(s) 38
Sse9I AATT 3 cut(s) 49, 392, 401
SsiI CCGC 1 cut(s) 243
StyI CCWWGG 2 cut(s) 173, 199
TaaI ACNGT 1 cut(s) 344
TaqI TCGA 2 cut(s) 147, 413
TasI AATT 3 cut(s) 49, 392, 401
TatI WGTACW 3 cut(s) 87, 276, 457
TauI GCSGC 1 cut(s) 245
TfiI GAWTC 1 cut(s) 638
Tru1I TTAA 1 cut(s) 338
Tru9I TTAA 1 cut(s) 338
TscAI CASTG 1 cut(s) 256
TseFI GTSAC 3 cut(s) 319, 344, 596
TseI GCWGC 1 cut(s) 21
Tsp45I GTSAC 3 cut(s) 319, 344, 596
TspDTI ATGAA 3 cut(s) 169, 292, 630
TspGWI ACGGA 1 cut(s) 501
TspRI CASTG 1 cut(s) 256
XapI RAATTY 1 cut(s) 401
XceI RCATGY 2 cut(s) 127, 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.